Rorug06G0376500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013622
Physical Location & Seq
Forward (+)
52294125 .. 52306827
12703 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0376500.1

Sequence Viewer

Length: 3174 bp
ATGTTCCATACAAATCGTACTCTCCACCCAGTTACAGTTCCCGCCATGGCCTCTACTACTACTACTAGTCTCCAAATCCCCACTTTCCTCTCCCTCAAACCACTCCCTCAGCGCCCTACCTTCGCCTTCTCCTCCCGCCCTCCACGCCGTCGTCTCACTCTCTCTCCCGCCGCCTCTCTCAAGGAGAATCTGGGCCGGATTGGAAAGACTTGGAACGACGTGACTAGCTTGAACAGCTGGGTTGTTCGAGACTACTACCGCCTTGTCAATTCTGTCAACTCTTTTGAGCCACAGCTACAGAGGCTCACTCATGACCAGCTTACTGCTAAAACTGCCGAGTTTCGGCGAAGATTGGGACAAGGAGAGACACTTGCGGATATTCAAGCTGAGGCTTTTGCTGTTGTTCGTGAAGCTGCGAAACGGAAGCTTGGTATGCGTCATTTCGATGTTCAGATTATTGGTGGGGCGGTGCTTCATGATGGGTCCATTGCAGAAATGAAGACCGGAGAGGGAAAAACGTTGGTTTCCACATTAGCAGCATATCTTAATGCCCTGACTGGCGAAGGTGTTCATGTGGTAACCGTAAATGATTACCTTGCTCAAAGGGATGCTGAGTGGATGGGTCGTGTTCATCGCTTCCTAGGTCTTTCAGTGGGTCTTATTCAGAGAGGGATGACAGCTGACAAGAGGAGGTCGAACTATAGATGTGACATAACATACACCAATAATTCAGAACTTGGGTTTGACTATCTACGAGACAATCTTGCCGGAAACAGTGGAGAACTTGTTATGAGATCGCCAAAGCCATTTCATTTTGCAATAGTTGATGAAGTTGACTCAGTTCTTATTGATGAAGGAAGAAATCCATTGCTAATTAGTGGCGAGGCTAGTAAAGATGCTGCTCGCTATCCTGTTGCTGCTAAAGTGGCTGAGTTGCTTGTGCGAGGAATTCATTACAATGTAGAACTTAAAGATTACTCAGTTGAGTTGACTGAAGAAGGAATAGCTCTTGCTGAGATGGCTCTTGAAACAAATGACTTATGGGATGAAAATGATCCCTGGGCCAGATTTGTGATGAATGCGTTGAAAGCTAAAGAGTTCTATCGGAAGGATGTGCAATATATTGTCAGAAATGGGAAGGCTCTCATAATCAATGAGCTCACTGGCAGAGTCGAAGATAAGAGAAGGTGGTCTGAGGGTATTCATCAAGCTGTGGAGGGAAAAGAAGGTTTGAAGATTCAGGCTGATTCGGTTGTTATCGCACAAATCACATATCAATCTCTGTTCAAGCTCTACCCAAAGCTATCAGGGATGACTGGCACTGCAAAAACTGAAGAGAAAGAGTTCTTGAAAATGTTCCAAACACCAGTTATTGAAGTACCAACAAATTTGCCAAACATTCGTAATGATTTACCTATTCAAGCTTTTGCGACTGCTCAGGGGAAATGGGAATATGTTCGTCAAGAAGTGGAATACATGTTCAGGCAGGGGCGTCCTGTTTTGGTTGGCACCACCAGTGTTGAGTATTCTGAACACCTGTCTGATCTGTTGAGGGAACATAACATCCCACACAATGTTCTAAATGCACGGCCCAAGTATGCTGCAAGGGAGGCTGAAATTGTTGCCCAAGCTGGACGAAAACATGCCATTACTCTTTCTACTAATATGGCTGGCAGAGGCACTGACATAATCCTAGGGGGGAATCCTAAAATGCTTGCAAAAGAAATCATAGAAGACAGTTTGATTTCATTCCTGACACGAGAAGCTCCTAACATTGACATAGATGGAGAGGCAATTTCACAAAAGGTATTGTCAAAGATTAAGGTCGGTCCATCATCATTAGCTTTGCTGGCTAAGACAGCGTTAATGGCTAAATATGTTGGCAAAAATGAAGGGAAGAGTTGGACATATAAGGAGGCAAAATCCATGATATCAGAGTCTGTGGAAATGAGTCAATCAAGAAATTTGAAAGAGCTAGAGAGGCTTGTTGATGAACAGTCTGAGATATACCCTCTTGGCCCTACAATAGCACTTGCTTATCTGTCAGTTTTGAAGGATTGCGAGGTACACTGTTTCAAAGAAGGGTCTGAAGTGAAAAGACTTGGGGGACTTCATGTGATTGGAACATCTTTGCACGAGTCTCGTAGAATAGATAACCAGCTTCGTGGCAGAGCAGGAAGGCAAGGTGATCCAGGATCAACTCGATTTATGGTGAGCTTGCAGGATGAGATGTTTCAAAAGTTTAATTTTGATACTGAGTGGGCTGTGAGACTTATTTCAAAAATTACTGATGATGAGGATGTACCAATTGAAGGTGATGCCATTGTGAGACAGCTCCTGTCCCTGCAAATTAATGCTGAAAAGTACTTTTTTGGCATAAGAAAGAGCCTGGTTGAGTTTGATGAAGTATTAGAGGTGCAGAGAAAGCATGTTTATGAACTTCGGCAGTCAATTTTAACTGGTGATAATGAAAGTTGTTCCCAGCATGTATTCCAGTACATGCAAGCAGTGGTAGATGAAATCGTATTTAAAAATGTTGACGCCTTCAAGCATCCAAGAAAATGGAGTTTAAATAAGCTTTTGAAGGAGTTTATGGCAATTGCTGGGAAACTATTGGATGATTCATTTGCAGGAATTACTGAAGAAGCTTTGTTAGAATCTCTTGCGCAGTCACTTGAACTGAACTTTAAAGATATTGATGACATTCACCTTCCAAACTTGCCAAAACCTCCAAATGCCTTCAGAGGTATCCGCAAGAAGAACTCTTCATTGAAACGTTGGCTTGCTATATGCTCGGATGATTTGACCAAGAATGGAAGGTACCATGCTACTACTAACCTCCTCCGCAAGTACCTTGGAGACTATTTGATTGCTTCATATTTGGATGTTGTTCAAGACTCTGGTTATGATGATACATATGTGAAGGAAGTTGAAAGAGCAGTTATTGCGAAAACCCTTGATTGTTTCTGGAGGGATCACCTTGTAAACATGAACAGGCTAAGTTCAGCGGTGAATGTAAGGAGTTTTGGGCACAGGAATCCTCTTGAAGAATACAAGATTGATGGTTGTCGATTTTTCATATCTATGCTTAGTGCAACGCGAAGGTTGACTGTAGAGTCACTTTTGCAATATTGGTCCTCCCCAATGGAGTCTCTAGAAATTTTTGTATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378

Protein Analysis

1057

Amino Acids

119.49

Weight (kDa)

6.95

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecA_DEAD PF07517 79 - 449 6.2e-116 SecA DEAD-like domain
SecA_PP_bind PF01043 300 - 406 5.3e-29 SecA preprotein cross-linking domain
P-loop_SecA PF21090 465 - 752 9.4e-66 SecA P-loop domain
SecA_SW PF07516 769 - 1043 1.9e-41 SecA Wing and Scaffold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32690
malus_domestica MD08G1078600.v1.1 MD15G1065400.v1.1
prunus_persica Prupe.1G419500_v2.0.a1
pyrus_communis pycom08g06500 pycom15g06190
rosa_chinensis RchiOBHm_Chr6g0309981
rosa_laevigata RLG00000010519
rosa_multiflora Rmu_sc0000294.1_g000010
rosa_roxburghii Rroxscaffold_7G00158980
rosa_rugosa Rorug06G0376500 Rorug06G0376600
rosa_samantha Rh6AG490300 Rh6BG499800 Rh6CG504600 Rh6CG504700 Rh6DG490700
rosa_wichuraiana Rw6G042680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3115
Acc16I TGCGCA 1 cut(s) 2667
Acc65I GGTACC 1 cut(s) 2820
AccB1I GGYRCC 2 cut(s) 1508, 2820
AccII CGCG 1 cut(s) 3100
AclI AACGTT 2 cut(s) 518, 2776
AclWI GGATC 4 cut(s) 1049, 2183, 2203, 2982
AcsI RAATTY 4 cut(s) 948, 1387, 1963, 3159
AcuI CTGAAG 5 cut(s) 1014, 1353, 2109, 2661, 2725
AcyI GRCGYC 2 cut(s) 1492, 2541
AfaI GTAC 8 cut(s) 19, 1380, 2067, 2304, 2366, 2498, 2822, 2852
AfiI CCNNNNNNNGG 2 cut(s) 342, 2312
AflIII ACRYGT 1 cut(s) 1476
AhlI ACTAGT 1 cut(s) 65
AjiI CACGTC 1 cut(s) 220
AjnI CCWGG 3 cut(s) 1058, 2191, 2388
AloI GAACNNNNNNTCC 4 cut(s) 1463, 1495, 1548, 1580
Alw21I GWGCWC 1 cut(s) 1161
AlwI GGATC 4 cut(s) 1049, 2183, 2203, 2982
AlwNI CAGNNNCTG 2 cut(s) 1940, 2338
AoxI GGCC 5 cut(s) 48, 193, 1062, 1589, 2017
ApeKI GCWGC 5 cut(s) 413, 536, 899, 917, 1601
ApoI RAATTY 4 cut(s) 948, 1387, 1963, 3159
AseI ATTAAT 1 cut(s) 2352
Asp700I GAANNNNTTC 4 cut(s) 567, 1343, 1355, 1455
Asp718I GGTACC 1 cut(s) 2820
AspA2I CCTAGG 2 cut(s) 640, 1693
AspLEI GCGC 2 cut(s) 114, 2668
AspS9I GGNCC 7 cut(s) 193, 483, 1062, 1590, 1829, 2018, 3135
AsuHPI GGTGA 7 cut(s) 2198, 2224, 2327, 2474, 2699, 2969, 3022
AvaII GGWCC 3 cut(s) 483, 1829, 3135
AvrII CCTAGG 2 cut(s) 640, 1693
BaeGI GKGCMC 1 cut(s) 3033
BaeI ACNNNNGTAYC 2 cut(s) 2904, 2937
BanI GGYRCC 2 cut(s) 1508, 2820
BanII GRGCYC 1 cut(s) 1161
BauI CACGAG 2 cut(s) 1758, 2135
BbsI GAAGAC 2 cut(s) 506, 1740
Bbv12I GWGCWC 1 cut(s) 1161
BbvCI CCTCAGC 2 cut(s) 108, 387
BbvI GCAGC 5 cut(s) 400, 548, 886, 904, 1588
BccI CCATC 6 cut(s) 473, 613, 1012, 1778, 1840, 3056
BceAI ACGGC 2 cut(s) 132, 1604
BcgI CGANNNNNNTGC 2 cut(s) 2123, 2157
BciT130I CCWGG 3 cut(s) 1060, 2193, 2390
BciVI GTATCC 1 cut(s) 2759
BcuI ACTAGT 1 cut(s) 65
BfaI CTAG 7 cut(s) 66, 225, 641, 888, 1694, 1976, 3155
BfmI CTRYAG 3 cut(s) 296, 700, 3111
BfoI RGCGCY 1 cut(s) 115
BfuI GTATCC 1 cut(s) 2759
BisI GCNGC 6 cut(s) 171, 414, 537, 900, 918, 1602
BlnI CCTAGG 2 cut(s) 640, 1693
BlsI GCNGC 6 cut(s) 172, 415, 538, 901, 919, 1603
BmcAI AGTACT 1 cut(s) 2366
Bme1390I CCNGG 3 cut(s) 1060, 2193, 2390
Bme18I GGWCC 3 cut(s) 483, 1829, 3135
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 7 cut(s) 193, 483, 1062, 1590, 1829, 2018, 3135
BmiI GGNNCC 3 cut(s) 484, 1510, 2822
BmrFI CCNGG 3 cut(s) 1060, 2193, 2390
BmrI ACTGGG 1 cut(s) 23
BmsI GCATC 4 cut(s) 598, 886, 2308, 2560
BmuI ACTGGG 1 cut(s) 23
BpiI GAAGAC 2 cut(s) 506, 1740
BplI GAGNNNNNCTC 2 cut(s) 292, 324
BpmI CTGGAG 1 cut(s) 2989
Bpu10I CCTNAGC 3 cut(s) 108, 387, 1437
BpuEI CTTGAG 1 cut(s) 164
BsaHI GRCGYC 2 cut(s) 1492, 2541
BsaJI CCNNGG 6 cut(s) 45, 640, 1058, 1059, 1693, 2854
BsaWI WCCGGW 1 cut(s) 503
BsaXI ACNNNNNCTCC 4 cut(s) 148, 178, 771, 801
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 2312
Bse1I ACTGG 8 cut(s) 29, 562, 1168, 1321, 1367, 1515, 2464, 2494
Bse3DI GCAATG 2 cut(s) 486, 866
BseBI CCWGG 3 cut(s) 1060, 2193, 2390
BseDI CCNNGG 6 cut(s) 45, 640, 1058, 1059, 1693, 2854
BseLI CCNNNNNNNGG 2 cut(s) 342, 2312
BseMI GCAATG 2 cut(s) 486, 866
BseNI ACTGG 8 cut(s) 29, 562, 1168, 1321, 1367, 1515, 2464, 2494
BseRI GAGGAG 3 cut(s) 121, 703, 2831
BseSI GKGCMC 1 cut(s) 3033
BseXI GCAGC 5 cut(s) 400, 548, 886, 904, 1588
BseYI CCCAGC 3 cut(s) 237, 2481, 2603
BsgI GTGCAG 1 cut(s) 2438
Bsh1236I CGCG 1 cut(s) 3100
BshFI GGCC 5 cut(s) 50, 195, 1064, 1591, 2019
BshNI GGYRCC 2 cut(s) 1508, 2820
BsiHKAI GWGCWC 1 cut(s) 1161
BsiSI CCGG 3 cut(s) 196, 504, 768
BslFI GGGAC 3 cut(s) 369, 2121, 2326
BslI CCNNNNNNNGG 2 cut(s) 342, 2312
BsmBI CGTCTC 1 cut(s) 158
BsmFI GGGAC 3 cut(s) 369, 2121, 2326
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 5 cut(s) 50, 195, 1064, 1591, 2019
Bsp1286I GDGCHC 2 cut(s) 1161, 3033
Bsp143I GATC 6 cut(s) 794, 1054, 1543, 2188, 2195, 2974
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 5 cut(s) 50, 195, 1064, 1591, 2019
BspFNI CGCG 1 cut(s) 3100
BspHI TCATGA 3 cut(s) 310, 475, 3170
BspLI GGNNCC 3 cut(s) 484, 1510, 2822
BspPI GGATC 4 cut(s) 1049, 2183, 2203, 2982
BspT107I GGYRCC 2 cut(s) 1508, 2820
BsrDI GCAATG 2 cut(s) 486, 866
BsrI ACTGG 8 cut(s) 29, 562, 1168, 1321, 1367, 1515, 2464, 2494
BssECI CCNNGG 6 cut(s) 45, 640, 1058, 1059, 1693, 2854
BssMI GATC 6 cut(s) 794, 1054, 1543, 2188, 2195, 2974
BssNI GRCGYC 2 cut(s) 1492, 2541
BssSI CACGAG 2 cut(s) 1758, 2135
BssT1I CCWWGG 4 cut(s) 45, 640, 1693, 2854
Bst2BI CACGAG 2 cut(s) 1758, 2135
Bst2UI CCWGG 3 cut(s) 1060, 2193, 2390
Bst4CI ACNGT 7 cut(s) 37, 583, 776, 1739, 1998, 2072, 3112
Bst6I CTCTTC 3 cut(s) 1329, 1892, 2770
BstACI GRCGYC 2 cut(s) 1492, 2541
BstAPI GCANNNNNTGC 1 cut(s) 2945
BstC8I GCNNGC 7 cut(s) 904, 1672, 1716, 1851, 2219, 2505, 2784
BstDSI CCRYGG 1 cut(s) 45
BstEII GGTNACC 1 cut(s) 577
BstFNI CGCG 1 cut(s) 3100
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 2 cut(s) 114, 2668
BstKTI GATC 6 cut(s) 797, 1057, 1546, 2191, 2198, 2977
BstMBI GATC 6 cut(s) 794, 1054, 1543, 2188, 2195, 2974
BstNI CCWGG 3 cut(s) 1060, 2193, 2390
BstNSI RCATGY 5 cut(s) 1480, 1646, 2432, 2489, 2503
BstPI GGTNACC 1 cut(s) 577
BstSCI CCNGG 3 cut(s) 1058, 2191, 2388
BstSFI CTRYAG 3 cut(s) 296, 700, 3111
BstSLI GKGCMC 1 cut(s) 3033
BstUI CGCG 1 cut(s) 3100
BstV1I GCAGC 5 cut(s) 400, 548, 886, 904, 1588
BstV2I GAAGAC 2 cut(s) 506, 1740
BstXI CCANNNNNNTGG 2 cut(s) 2165, 2562
BsuI GTATCC 1 cut(s) 2759
BsuRI GGCC 5 cut(s) 50, 195, 1064, 1591, 2019
BtgI CCRYGG 1 cut(s) 45
BtgZI GCGATG 1 cut(s) 617
BtrI CACGTC 1 cut(s) 220
BtsI GCAGTG 2 cut(s) 1320, 2514
BtsIMutI CAGTG 8 cut(s) 657, 781, 1161, 1320, 1522, 1680, 2068, 2514
Cac8I GCNNGC 7 cut(s) 904, 1672, 1716, 1851, 2219, 2505, 2784
CaiI CAGNNNCTG 2 cut(s) 1940, 2338
CciI TCATGA 3 cut(s) 310, 475, 3170
CfoI GCGC 2 cut(s) 114, 2668
Cfr13I GGNCC 7 cut(s) 193, 483, 1062, 1590, 1829, 2018, 3135
CseI GACGC 3 cut(s) 425, 1481, 2549
Csp6I GTAC 8 cut(s) 18, 1379, 2066, 2303, 2365, 2497, 2821, 2851
CspCI CAANNNNNGTGG 2 cut(s) 1501, 1536
CviQI GTAC 8 cut(s) 18, 1379, 2066, 2303, 2365, 2497, 2821, 2851
DpnI GATC 6 cut(s) 796, 1056, 1545, 2190, 2197, 2976
DpnII GATC 6 cut(s) 794, 1054, 1543, 2188, 2195, 2974
DraI TTTAAA 3 cut(s) 2530, 2571, 2689
DrdI GACNNNNNNGTC 1 cut(s) 3115
DseDI GACNNNNNNGTC 1 cut(s) 3115
Eam1104I CTCTTC 3 cut(s) 1329, 1892, 2770
EarI CTCTTC 3 cut(s) 1329, 1892, 2770
Ecl136II GAGCTC 1 cut(s) 1159
Eco130I CCWWGG 4 cut(s) 45, 640, 1693, 2854
Eco24I GRGCYC 1 cut(s) 1161
Eco32I GATATC 1 cut(s) 1932
Eco47I GGWCC 3 cut(s) 483, 1829, 3135
Eco53kI GAGCTC 1 cut(s) 1159
Eco57I CTGAAG 5 cut(s) 1014, 1353, 2109, 2661, 2725
Eco91I GGTNACC 1 cut(s) 577
EcoICRI GAGCTC 1 cut(s) 1159
EcoO65I GGTNACC 1 cut(s) 577
EcoRI GAATTC 1 cut(s) 948
EcoRII CCWGG 3 cut(s) 1058, 2191, 2388
EcoRV GATATC 1 cut(s) 1932
EcoT14I CCWWGG 4 cut(s) 45, 640, 1693, 2854
EcoT38I GRGCYC 1 cut(s) 1161
ErhI CCWWGG 4 cut(s) 45, 640, 1693, 2854
Esp3I CGTCTC 1 cut(s) 158
FaqI GGGAC 3 cut(s) 369, 2121, 2326
FauI CCCGC 3 cut(s) 49, 143, 175
FauNDI CATATG 1 cut(s) 2917
Fnu4HI GCNGC 6 cut(s) 171, 414, 537, 900, 918, 1602
FriOI GRGCYC 1 cut(s) 1161
Fsp4HI GCNGC 6 cut(s) 171, 414, 537, 900, 918, 1602
FspBI CTAG 7 cut(s) 66, 225, 641, 888, 1694, 1976, 3155
FspI TGCGCA 1 cut(s) 2667
GlaI GCGC 2 cut(s) 113, 2667
GluI GCNGC 6 cut(s) 171, 414, 537, 900, 918, 1602
GsaI CCCAGC 3 cut(s) 241, 2485, 2607
GsuI CTGGAG 1 cut(s) 2989
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 5 cut(s) 50, 195, 1064, 1591, 2019
HapII CCGG 3 cut(s) 196, 504, 768
HgaI GACGC 3 cut(s) 425, 1481, 2549
HhaI GCGC 2 cut(s) 114, 2668
Hin1I GRCGYC 2 cut(s) 1492, 2541
Hin6I GCGC 2 cut(s) 112, 2666
HinP1I GCGC 2 cut(s) 112, 2666
HincII GTYRAC 5 cut(s) 277, 835, 990, 2539, 3108
HindII GTYRAC 5 cut(s) 277, 835, 990, 2539, 3108
HindIII AAGCTT 4 cut(s) 425, 1422, 2576, 2646
HpaII CCGG 3 cut(s) 196, 504, 768
HphI GGTGA 7 cut(s) 2198, 2224, 2327, 2474, 2699, 2969, 3022
Hpy166II GTNNAC 7 cut(s) 277, 835, 990, 2068, 2539, 2986, 3108
Hpy8I GTNNAC 7 cut(s) 277, 835, 990, 2068, 2539, 2986, 3108
Hpy99I CGWCG 2 cut(s) 153, 221
HpyCH4III ACNGT 7 cut(s) 37, 583, 776, 1739, 1998, 2072, 3112
HpyCH4IV ACGT 3 cut(s) 219, 518, 2776
HpySE526I ACGT 3 cut(s) 219, 518, 2776
Hsp92I GRCGYC 2 cut(s) 1492, 2541
HspAI GCGC 2 cut(s) 112, 2666
KpnI GGTACC 1 cut(s) 2824
Kzo9I GATC 6 cut(s) 794, 1054, 1543, 2188, 2195, 2974
LmnI GCTCC 2 cut(s) 1771, 2340
Lsp1109I GCAGC 5 cut(s) 400, 548, 886, 904, 1588
LweI GCATC 4 cut(s) 598, 886, 2308, 2560
MaeI CTAG 7 cut(s) 66, 225, 641, 888, 1694, 1976, 3155
MaeII ACGT 3 cut(s) 219, 518, 2776
MaeIII GTNAC 6 cut(s) 31, 220, 577, 707, 2670, 3117
MalI GATC 6 cut(s) 796, 1056, 1545, 2190, 2197, 2976
MboI GATC 6 cut(s) 794, 1054, 1543, 2188, 2195, 2974
MfeI CAATTG 2 cut(s) 2307, 2598
MhlI GDGCHC 2 cut(s) 1161, 3033
MlyI GAGTC 8 cut(s) 830, 1179, 1946, 1960, 2147, 2891, 3125, 3158
MmeI TCCRAC 1 cut(s) 1883
MroXI GAANNNNTTC 4 cut(s) 567, 1343, 1355, 1455
MslI CAYNNNNRTG 4 cut(s) 444, 957, 2433, 3083
MspA1I CMGCKG 3 cut(s) 237, 680, 3008
MspI CCGG 3 cut(s) 196, 504, 768
MspR9I CCNGG 3 cut(s) 1060, 2193, 2390
MunI CAATTG 2 cut(s) 2307, 2598
Mva1269I GAATGC 1 cut(s) 1084
MvaI CCWGG 3 cut(s) 1060, 2193, 2390
MvnI CGCG 1 cut(s) 3100
NcoI CCATGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 2917
NdeII GATC 6 cut(s) 794, 1054, 1543, 2188, 2195, 2974
NlaIV GGNNCC 3 cut(s) 484, 1510, 2822
NmeAIII GCCGAG 1 cut(s) 361
NmuCI GTSAC 4 cut(s) 220, 707, 2670, 3117
NsbI TGCGCA 1 cut(s) 2667
NspI RCATGY 5 cut(s) 1480, 1646, 2432, 2489, 2503
PagI TCATGA 3 cut(s) 310, 475, 3170
PasI CCCWGGG 1 cut(s) 1059
PciI ACATGT 1 cut(s) 1476
PctI GAATGC 1 cut(s) 1084
PdmI GAANNNNTTC 4 cut(s) 567, 1343, 1355, 1455
PfeI GAWTC 7 cut(s) 187, 1237, 1247, 1702, 2621, 2657, 3037
PfoI TCCNGGA 1 cut(s) 2191
PkrI GCNGC 6 cut(s) 172, 415, 538, 901, 919, 1603
PleI GAGTC 8 cut(s) 830, 1178, 1945, 1959, 2146, 2891, 3124, 3157
PpsI GAGTC 8 cut(s) 830, 1178, 1945, 1959, 2146, 2891, 3124, 3157
PscI ACATGT 1 cut(s) 1476
PshBI ATTAAT 1 cut(s) 2352
Psp124BI GAGCTC 1 cut(s) 1161
Psp1406I AACGTT 2 cut(s) 518, 2776
Psp6I CCWGG 3 cut(s) 1058, 2191, 2388
PspEI GGTNACC 1 cut(s) 577
PspFI CCCAGC 3 cut(s) 237, 2481, 2603
PspGI CCWGG 3 cut(s) 1058, 2191, 2388
PspN4I GGNNCC 3 cut(s) 484, 1510, 2822
PspPI GGNCC 7 cut(s) 193, 483, 1062, 1590, 1829, 2018, 3135
PstNI CAGNNNCTG 2 cut(s) 1940, 2338
PvuII CAGCTG 2 cut(s) 237, 680
RsaI GTAC 8 cut(s) 19, 1380, 2067, 2304, 2366, 2498, 2822, 2852
RsaNI GTAC 8 cut(s) 18, 1379, 2066, 2303, 2365, 2497, 2821, 2851
RseI CAYNNNNRTG 4 cut(s) 444, 957, 2433, 3083
SacI GAGCTC 1 cut(s) 1161
SatI GCNGC 6 cut(s) 171, 414, 537, 900, 918, 1602
Sau3AI GATC 6 cut(s) 794, 1054, 1543, 2188, 2195, 2974
Sau96I GGNCC 7 cut(s) 193, 483, 1062, 1590, 1829, 2018, 3135
ScaI AGTACT 1 cut(s) 2366
SchI GAGTC 8 cut(s) 830, 1179, 1946, 1960, 2147, 2891, 3125, 3158
ScrFI CCNGG 3 cut(s) 1060, 2193, 2390
SduI GDGCHC 2 cut(s) 1161, 3033
SfaNI GCATC 4 cut(s) 598, 886, 2308, 2560
SfcI CTRYAG 3 cut(s) 296, 700, 3111
SinI GGWCC 3 cut(s) 483, 1829, 3135
SmiMI CAYNNNNRTG 4 cut(s) 444, 957, 2433, 3083
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SpeI ACTAGT 1 cut(s) 65
SspI AATATT 1 cut(s) 3131
SspMI CTAG 7 cut(s) 66, 225, 641, 888, 1694, 1976, 3155
SstI GAGCTC 1 cut(s) 1161
StyD4I CCNGG 3 cut(s) 1058, 2191, 2388
StyI CCWWGG 4 cut(s) 45, 640, 1693, 2854
TaaI ACNGT 7 cut(s) 37, 583, 776, 1739, 1998, 2072, 3112
TaiI ACGT 3 cut(s) 222, 521, 2779
TaqI TCGA 6 cut(s) 247, 444, 695, 1173, 2203, 3070
TaqII GACCGA 1 cut(s) 1817
TatI WGTACW 2 cut(s) 2364, 2496
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 7 cut(s) 187, 1237, 1247, 1702, 2621, 2657, 3037
TscAI CASTG 8 cut(s) 657, 781, 1168, 1327, 1522, 1687, 2075, 2514
TseFI GTSAC 4 cut(s) 220, 707, 2670, 3117
TseI GCWGC 5 cut(s) 413, 536, 899, 917, 1601
Tsp45I GTSAC 4 cut(s) 220, 707, 2670, 3117
TspGWI ACGGA 1 cut(s) 436
TspRI CASTG 8 cut(s) 657, 781, 1168, 1327, 1522, 1687, 2075, 2514
VpaK11BI GGWCC 3 cut(s) 483, 1829, 3135
VspI ATTAAT 1 cut(s) 2352
XapI RAATTY 4 cut(s) 948, 1387, 1963, 3159
XbaI TCTAGA 1 cut(s) 3154
XceI RCATGY 5 cut(s) 1480, 1646, 2432, 2489, 2503
XmaJI CCTAGG 2 cut(s) 640, 1693
XmnI GAANNNNTTC 4 cut(s) 567, 1343, 1355, 1455
XspI CTAG 7 cut(s) 66, 225, 641, 888, 1694, 1976, 3155
ZrmI AGTACT 1 cut(s) 2366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.