pycom08g06500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013622
Physical Location & Seq
Reverse (-)
5070020 .. 5070709
690 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g06500.2

Sequence Viewer

Length: 555 bp
ATGGCTTCCACCCCCAAGCCGGTAAAACTTGCAGAGCTCCTCCAAGAGCAGCAAGAGCCCTTTGTCCTAGAGGTTTACCTATTTGAAAGGAGTTACCTGAGAAAAACCTCGAGTACAAATAGCAGTTCGAGGATTACAAGTAGAACTTCTAGCAAGAAACTCACCAGATCATCCAGTTGGGGTAATCTCAGCTGCCATAAAGTATTGAGATCTGTGTGTAACAAGCTTGGTTCAAGACATAGTGAGACAACAAGCACTAAGGGTTGTGAAAACCGAGAGGGGAAGCCCAATGGTGGCGCCGAGACGCGGAGAAGTAGCCGAGAGAGCGGGGGGACGGATACATTTTCATCTGCCAGCAGTAGGACACAGTATGATTCGTGCTCCGAGGGCGAAAAAGAATCAAAAGAAGAAGCTCCCGTTTCGTTGCGAAATAAGCATGATGCTTCCTCACTTGCTGCAGACGCTACTCAAATTTCGGAATCCTGTAATATGAAAGAGAGAAAGGATGTTACAGATAGAATGGCTCAACGGACAATCATCGAAGATTCACCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378

Protein Analysis

185

Amino Acids

20.34

Weight (kDa)

8.99

Isoelectric Point (pI)

74.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32690
malus_domestica MD08G1078600.v1.1 MD15G1065400.v1.1
prunus_persica Prupe.1G419500_v2.0.a1
pyrus_communis pycom08g06500 pycom15g06190
rosa_chinensis RchiOBHm_Chr6g0309981
rosa_laevigata RLG00000010519
rosa_multiflora Rmu_sc0000294.1_g000010
rosa_roxburghii Rroxscaffold_7G00158980
rosa_rugosa Rorug06G0376500 Rorug06G0376600
rosa_samantha Rh6AG490300 Rh6BG499800 Rh6CG504600 Rh6CG504700 Rh6DG490700
rosa_wichuraiana Rw6G042680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 296
AccBSI CCGCTC 1 cut(s) 327
AccII CGCG 1 cut(s) 307
AciI CCGC 2 cut(s) 307, 327
AcsI RAATTY 1 cut(s) 471
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 4 cut(s) 19, 293, 306, 360
AgsI TTSAA 2 cut(s) 86, 234
AluBI AGCT 4 cut(s) 37, 192, 226, 413
AluI AGCT 4 cut(s) 37, 192, 226, 413
Alw21I GWGCWC 2 cut(s) 39, 383
Alw26I GTCTC 2 cut(s) 239, 296
Ama87I CYCGRG 1 cut(s) 109
ApeKI GCWGC 3 cut(s) 49, 192, 455
ApoI RAATTY 1 cut(s) 471
AspLEI GCGC 1 cut(s) 299
AsuHPI GGTGA 2 cut(s) 154, 540
AvaI CYCGRG 1 cut(s) 109
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 2 cut(s) 39, 60
Bbv12I GWGCWC 2 cut(s) 39, 383
BbvI GCAGC 3 cut(s) 61, 179, 442
BciVI GTATCC 1 cut(s) 331
BcoDI GTCTC 2 cut(s) 239, 296
BfaI CTAG 2 cut(s) 68, 150
BfmI CTRYAG 1 cut(s) 456
BfoI RGCGCY 1 cut(s) 300
BfuI GTATCC 1 cut(s) 331
BglII AGATCT 1 cut(s) 209
BisI GCNGC 3 cut(s) 50, 193, 456
BlsI GCNGC 3 cut(s) 51, 194, 457
BmeT110I CYCGRG 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 298
BmsI GCATC 1 cut(s) 430
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 384
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 293, 306, 360
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 384
BseGI GGATG 2 cut(s) 170, 511
BseLI CCNNNNNNNGG 4 cut(s) 19, 293, 306, 360
BseMII CTCAG 2 cut(s) 89, 202
BseNI ACTGG 1 cut(s) 174
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 3 cut(s) 61, 179, 442
Bsh1236I CGCG 1 cut(s) 307
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 2 cut(s) 39, 383
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 4 cut(s) 19, 293, 306, 360
BsmAI GTCTC 2 cut(s) 239, 296
BsmBI CGTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 346
BsoBI CYCGRG 1 cut(s) 109
Bsp1286I GDGCHC 3 cut(s) 39, 60, 383
Bsp143I GATC 2 cut(s) 167, 209
BspACI CCGC 2 cut(s) 307, 327
BspCNI CTCAG 2 cut(s) 90, 201
BspFNI CGCG 1 cut(s) 307
BspLI GGNNCC 1 cut(s) 298
BspMAI CTGCAG 1 cut(s) 460
BspT107I GGYRCC 1 cut(s) 296
BsrBI CCGCTC 1 cut(s) 327
BsrFI RCCGGY 1 cut(s) 19
BsrI ACTGG 1 cut(s) 174
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 2 cut(s) 167, 209
BssNI GRCGYC 1 cut(s) 297
Bst4CI ACNGT 2 cut(s) 369, 552
BstACI GRCGYC 1 cut(s) 297
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 3 cut(s) 98, 188, 258
BstF5I GGATG 2 cut(s) 170, 511
BstFNI CGCG 1 cut(s) 307
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 2 cut(s) 170, 212
BstMAI GTCTC 2 cut(s) 239, 296
BstMBI GATC 2 cut(s) 167, 209
BstMWI GCNNNNNNNGC 5 cut(s) 55, 324, 387, 433, 461
BstSFI CTRYAG 1 cut(s) 456
BstUI CGCG 1 cut(s) 307
BstV1I GCAGC 3 cut(s) 61, 179, 442
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
BsuI GTATCC 1 cut(s) 331
BtsCI GGATG 2 cut(s) 170, 511
Cac8I GCNNGC 1 cut(s) 355
CfoI GCGC 1 cut(s) 299
Cfr10I RCCGGY 1 cut(s) 19
CseI GACGC 2 cut(s) 313, 470
Csp6I GTAC 1 cut(s) 114
CviAII CATG 1 cut(s) 437
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 3 cut(s) 98, 188, 258
DinI GGCGCC 1 cut(s) 298
DpnI GATC 2 cut(s) 169, 211
DpnII GATC 2 cut(s) 167, 209
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 2 cut(s) 39, 60
Eco53kI GAGCTC 1 cut(s) 37
Eco88I CYCGRG 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 37
EcoT38I GRGCYC 2 cut(s) 39, 60
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
Esp3I CGTCTC 1 cut(s) 296
FaeI CATG 1 cut(s) 440
FaiI YATR 5 cut(s) 198, 240, 372, 438, 491
FalI AAGNNNNNCTT 2 cut(s) 130, 162
FaqI GGGAC 1 cut(s) 346
FatI CATG 1 cut(s) 436
FauI CCCGC 1 cut(s) 320
Fnu4HI GCNGC 3 cut(s) 50, 193, 456
FokI GGATG 2 cut(s) 157, 518
FriOI GRGCYC 2 cut(s) 39, 60
Fsp4HI GCNGC 3 cut(s) 50, 193, 456
FspBI CTAG 2 cut(s) 68, 150
GlaI GCGC 1 cut(s) 298
GluI GCNGC 3 cut(s) 50, 193, 456
HaeII RGCGCY 1 cut(s) 300
HapII CCGG 1 cut(s) 20
HgaI GACGC 2 cut(s) 313, 470
HhaI GCGC 1 cut(s) 299
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 1 cut(s) 440
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 4 cut(s) 374, 398, 479, 545
HpaII CCGG 1 cut(s) 20
HphI GGTGA 2 cut(s) 154, 540
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 385, 478
Hpy188III TCNNGA 1 cut(s) 234
Hpy8I GTNNAC 1 cut(s) 76
HpyCH4III ACNGT 2 cut(s) 369, 552
HpyCH4V TGCA 2 cut(s) 32, 458
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 324, 387, 433, 461
HpyF3I CTNAG 3 cut(s) 98, 188, 258
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 1 cut(s) 440
HspAI GCGC 1 cut(s) 297
KasI GGCGCC 1 cut(s) 296
Kzo9I GATC 2 cut(s) 167, 209
LmnI GCTCC 3 cut(s) 42, 386, 418
LpnPI CCDG 6 cut(s) 33, 110, 178, 187, 367, 496
Lsp1109I GCAGC 3 cut(s) 61, 179, 442
LweI GCATC 1 cut(s) 430
MaeI CTAG 2 cut(s) 68, 150
MaeIII GTNAC 3 cut(s) 92, 218, 508
MalI GATC 2 cut(s) 169, 211
MbiI CCGCTC 1 cut(s) 327
MboI GATC 2 cut(s) 167, 209
MboII GAAGA 2 cut(s) 419, 554
MflI RGATCY 1 cut(s) 209
MhlI GDGCHC 3 cut(s) 39, 60, 383
MluCI AATT 1 cut(s) 471
Mly113I GGCGCC 1 cut(s) 297
MnlI CCTC 7 cut(s) 50, 64, 118, 123, 271, 379, 457
MspA1I CMGCKG 1 cut(s) 192
MspI CCGG 1 cut(s) 20
MvnI CGCG 1 cut(s) 307
MwoI GCNNNNNNNGC 5 cut(s) 55, 324, 387, 433, 461
NarI GGCGCC 1 cut(s) 297
NdeII GATC 2 cut(s) 167, 209
NlaIII CATG 1 cut(s) 440
NlaIV GGNNCC 1 cut(s) 298
NmeAIII GCCGAG 2 cut(s) 325, 344
PaeR7I CTCGAG 1 cut(s) 109
PfeI GAWTC 4 cut(s) 374, 398, 479, 545
PkrI GCNGC 3 cut(s) 51, 194, 457
PluTI GGCGCC 1 cut(s) 300
Psp124BI GAGCTC 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 298
PspXI VCTCGAGB 1 cut(s) 109
PstI CTGCAG 1 cut(s) 460
PsuI RGATCY 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 192
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
SacI GAGCTC 1 cut(s) 39
SatI GCNGC 3 cut(s) 50, 193, 456
Sau3AI GATC 2 cut(s) 167, 209
SduI GDGCHC 3 cut(s) 39, 60, 383
SetI ASST 8 cut(s) 39, 75, 81, 99, 110, 194, 228, 415
SfaNI GCATC 1 cut(s) 430
SfcI CTRYAG 1 cut(s) 456
SfoI GGCGCC 1 cut(s) 298
Sfr274I CTCGAG 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 109
SmoI CTYRAG 1 cut(s) 109
Sse9I AATT 1 cut(s) 471
SsiI CCGC 2 cut(s) 307, 327
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 2 cut(s) 68, 150
SstI GAGCTC 1 cut(s) 39
TaaI ACNGT 2 cut(s) 369, 552
TaqI TCGA 3 cut(s) 110, 128, 540
TasI AATT 1 cut(s) 471
TatI WGTACW 1 cut(s) 113
TfiI GAWTC 4 cut(s) 374, 398, 479, 545
TseI GCWGC 3 cut(s) 49, 192, 455
TspDTI ATGAA 2 cut(s) 336, 506
TspGWI ACGGA 2 cut(s) 350, 544
XapI RAATTY 1 cut(s) 471
XhoI CTCGAG 1 cut(s) 109
XspI CTAG 2 cut(s) 68, 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.