RLG00000010519

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013622
Physical Location & Seq
Reverse (-)
2339037 .. 2340340
1304 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010519

Sequence Viewer

Length: 1098 bp
ATGGCTTCCACAACAACAAAACCAGTTAAACAACTTGGAGAGCTTCTCCAAGAGCAGCAAGAACCCTTTGTCCTAGATGTTTACCTATTTGAAAGAGGGTGTCTGAGAAAGAGCTTGAGTTCAAGCAATAGCATTAATTCAAGTAAGAGTTTAAAGAGATCCTCTAGTTGTGGACCAATCAAGAGCAAAAAGGATACTCCTAGCTTCTCCAAAATACTGAGATCTGTCTATAACAAGCTTGTTTCGAGAACTAAGAGTTCCGGAAAAGAAGAGGGAAAGTTTGATGCCAACACTGAGATTACTCTTCCTAGTGATTGCCTTGGAGATTTGGATGGATTTTCTTCTTCTAGTAGCAGAACACAGTATGAATCATGCTGTGATAGTGACAAAGATGAAGCTTGCGCCACTGTTTTGTTGCAGAATGAAGATGAAGCCTCGCTTGCAGTAGAAGGCACTTCTCAAGTTCCAAATCTCTGCAATATGAAAAACGAGACAAAGATAGCACTGCTCAATGGGGAATCATCGAAGAAGAAGAAGAAGAAGACGCAGTCTAAGCAACACACCTCAATTTCAGTACTAGAAGATAGACCTTCTCATGCAGTACCCAACAAGGTCATGGAAAACTCCATGTTATCAGCTTCTCTTTGGGAACTACTTTTCCAGCCACCATTGGAGAGGCCAAGAGATACTTTTGGAGTTCCGGAAATGCTAGAGCCGGTAATCAGATCGAATTCTTCTCCACACAAATCGAAAAGGATGTTGCAGCAGACAAGGCAGCTTCTTTTCGATTGTGTGAGAGAGGTGACAGAATCCCATGCAAAGCAAATGAAAGAGGAGCAGAACAGCACAAAGTTTTTGGGGGCAGAAGAACTGGGAAAGCTGATTTCTGAGAAACTAAGGATTTGGGATAGACAGGCTGGGGATGAAACCAGCATCGACTTTTTGTTGGATTCAGATATCCTGAGTTCAGCTGAAGAATGGAACTGTAATGAACAAGAAGAGAAAGAGATTTGTTGCGAAATCGGGGACGCCATTTTAGAAGAGATCATCAAAGAAACTGTTGCTCTACCGTTTAGTTTAGATCCTGTTTCCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378

Protein Analysis

366

Amino Acids

40.79

Weight (kDa)

5.23

Isoelectric Point (pI)

62.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 248 - 352 3.2e-08 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32690
malus_domestica MD08G1078600.v1.1 MD15G1065400.v1.1
prunus_persica Prupe.1G419500_v2.0.a1
pyrus_communis pycom08g06500 pycom15g06190
rosa_chinensis RchiOBHm_Chr6g0309981
rosa_laevigata RLG00000010519
rosa_multiflora Rmu_sc0000294.1_g000010
rosa_roxburghii Rroxscaffold_7G00158980
rosa_rugosa Rorug06G0376500 Rorug06G0376600
rosa_samantha Rh6AG490300 Rh6BG499800 Rh6CG504600 Rh6CG504700 Rh6DG490700
rosa_wichuraiana Rw6G042680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 260, 700
AclWI GGATC 2 cut(s) 153, 1076
AcsI RAATTY 1 cut(s) 730
AcuI CTGAAG 1 cut(s) 993
AcyI GRCGYC 1 cut(s) 1029
AfaI GTAC 2 cut(s) 576, 603
AgsI TTSAA 3 cut(s) 92, 123, 141
AloI GAACNNNNNNTCC 4 cut(s) 54, 86, 642, 674
AluBI AGCT 9 cut(s) 43, 114, 204, 238, 398, 638, 778, 880, 971
AluI AGCT 9 cut(s) 43, 114, 204, 238, 398, 638, 778, 880, 971
Alw26I GTCTC 1 cut(s) 485
AlwI GGATC 2 cut(s) 153, 1076
Aor13HI TCCGGA 2 cut(s) 260, 700
AoxI GGCC 1 cut(s) 677
ApeKI GCWGC 3 cut(s) 55, 763, 775
ApoI RAATTY 1 cut(s) 730
AseI ATTAAT 1 cut(s) 135
AspLEI GCGC 1 cut(s) 404
AspS9I GGNCC 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 814
AvaII GGWCC 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 548
BbvI GCAGC 3 cut(s) 67, 775, 787
BccI CCATC 1 cut(s) 326
BciVI GTATCC 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 485
BfaI CTAG 7 cut(s) 74, 165, 201, 309, 348, 578, 710
BfuI GTATCC 1 cut(s) 187
BglII AGATCT 1 cut(s) 221
BisI GCNGC 3 cut(s) 56, 764, 776
BlsI GCNGC 3 cut(s) 57, 765, 777
BmcAI AGTACT 1 cut(s) 576
Bme18I GGWCC 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 173
BmrI ACTGGG 1 cut(s) 881
BmsI GCATC 2 cut(s) 274, 942
BmuI ACTGGG 1 cut(s) 881
BpiI GAAGAC 1 cut(s) 548
BplI GAGNNNNNCTC 2 cut(s) 30, 62
BpuEI CTTGAG 2 cut(s) 136, 444
BsaHI GRCGYC 1 cut(s) 1029
BsaJI CCNNGG 1 cut(s) 319
BsaWI WCCGGW 2 cut(s) 260, 700
Bse118I RCCGGY 1 cut(s) 715
Bse1I ACTGG 2 cut(s) 23, 876
BseAI TCCGGA 2 cut(s) 260, 700
BseDI CCNNGG 1 cut(s) 319
BseGI GGATG 3 cut(s) 337, 762, 928
BseMII CTCAG 5 cut(s) 95, 209, 285, 879, 953
BseNI ACTGG 2 cut(s) 23, 876
BseRI GAGGAG 1 cut(s) 848
BseXI GCAGC 3 cut(s) 67, 775, 787
BseYI CCCAGC 1 cut(s) 917
BshFI GGCC 1 cut(s) 679
BsiSI CCGG 3 cut(s) 261, 701, 716
BslFI GGGAC 1 cut(s) 1040
BsmAI GTCTC 1 cut(s) 485
BsmFI GGGAC 1 cut(s) 1040
BsnI GGCC 1 cut(s) 679
Bsp13I TCCGGA 2 cut(s) 260, 700
Bsp143I GATC 5 cut(s) 158, 221, 725, 1044, 1081
BspANI GGCC 1 cut(s) 679
BspCNI CTCAG 5 cut(s) 96, 210, 286, 880, 954
BspEI TCCGGA 2 cut(s) 260, 700
BspPI GGATC 2 cut(s) 153, 1076
BsrFI RCCGGY 1 cut(s) 715
BsrI ACTGG 2 cut(s) 23, 876
BssAI RCCGGY 1 cut(s) 715
BssECI CCNNGG 1 cut(s) 319
BssMI GATC 5 cut(s) 158, 221, 725, 1044, 1081
BssNI GRCGYC 1 cut(s) 1029
BssT1I CCWWGG 1 cut(s) 319
Bst4CI ACNGT 5 cut(s) 363, 409, 986, 1060, 1071
Bst6I CTCTTC 4 cut(s) 264, 309, 993, 1035
BstACI GRCGYC 1 cut(s) 1029
BstC8I GCNNGC 2 cut(s) 400, 441
BstDEI CTNAG 8 cut(s) 104, 218, 252, 294, 552, 888, 896, 962
BstF5I GGATG 3 cut(s) 337, 762, 928
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 5 cut(s) 161, 224, 728, 1047, 1084
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 5 cut(s) 158, 221, 725, 1044, 1081
BstMWI GCNNNNNNNGC 3 cut(s) 440, 553, 772
BstV1I GCAGC 3 cut(s) 67, 775, 787
BstV2I GAAGAC 1 cut(s) 548
BstX2I RGATCY 3 cut(s) 158, 221, 1081
BstYI RGATCY 3 cut(s) 158, 221, 1081
BsuI GTATCC 1 cut(s) 187
BsuRI GGCC 1 cut(s) 679
BtsCI GGATG 3 cut(s) 337, 762, 928
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 3 cut(s) 291, 405, 503
Cac8I GCNNGC 2 cut(s) 400, 441
CfoI GCGC 1 cut(s) 404
Cfr10I RCCGGY 1 cut(s) 715
Cfr13I GGNCC 1 cut(s) 173
CseI GACGC 2 cut(s) 553, 1037
Csp6I GTAC 2 cut(s) 575, 602
CviAII CATG 5 cut(s) 372, 596, 616, 628, 815
CviQI GTAC 2 cut(s) 575, 602
DdeI CTNAG 8 cut(s) 104, 218, 252, 294, 552, 888, 896, 962
DpnI GATC 5 cut(s) 160, 223, 727, 1046, 1083
DpnII GATC 5 cut(s) 158, 221, 725, 1044, 1081
DraI TTTAAA 1 cut(s) 153
Eam1104I CTCTTC 4 cut(s) 264, 309, 993, 1035
EarI CTCTTC 4 cut(s) 264, 309, 993, 1035
Eco130I CCWWGG 1 cut(s) 319
Eco32I GATATC 1 cut(s) 958
Eco47I GGWCC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 993
EcoRI GAATTC 1 cut(s) 730
EcoRV GATATC 1 cut(s) 958
EcoT14I CCWWGG 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 5 cut(s) 375, 599, 619, 631, 818
FaiI YATR 8 cut(s) 231, 366, 373, 482, 597, 617, 629, 816
FalI AAGNNNNNCTT 4 cut(s) 423, 455, 673, 705
FaqI GGGAC 1 cut(s) 1040
FatI CATG 5 cut(s) 371, 595, 615, 627, 814
Fnu4HI GCNGC 3 cut(s) 56, 764, 776
FokI GGATG 3 cut(s) 344, 769, 935
Fsp4HI GCNGC 3 cut(s) 56, 764, 776
FspBI CTAG 7 cut(s) 74, 165, 201, 309, 348, 578, 710
GlaI GCGC 1 cut(s) 403
GluI GCNGC 3 cut(s) 56, 764, 776
GsaI CCCAGC 1 cut(s) 921
HaeIII GGCC 1 cut(s) 679
HapII CCGG 3 cut(s) 261, 701, 716
HgaI GACGC 2 cut(s) 553, 1037
HhaI GCGC 1 cut(s) 404
Hin1I GRCGYC 1 cut(s) 1029
Hin1II CATG 5 cut(s) 375, 599, 619, 631, 818
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HindIII AAGCTT 2 cut(s) 236, 396
HinfI GANTC 4 cut(s) 368, 518, 809, 950
HpaII CCGG 3 cut(s) 261, 701, 716
HphI GGTGA 1 cut(s) 814
Hpy166II GTNNAC 2 cut(s) 82, 173
Hpy188I TCNGA 5 cut(s) 105, 725, 889, 955, 1097
Hpy188III TCNNGA 5 cut(s) 181, 246, 261, 701, 961
Hpy8I GTNNAC 2 cut(s) 82, 173
HpyAV CCTTC 2 cut(s) 443, 600
HpyCH4III ACNGT 5 cut(s) 363, 409, 986, 1060, 1071
HpyCH4V TGCA 6 cut(s) 418, 443, 477, 599, 763, 818
HpyF10VI GCNNNNNNNGC 3 cut(s) 440, 553, 772
HpyF3I CTNAG 8 cut(s) 104, 218, 252, 294, 552, 888, 896, 962
Hsp92I GRCGYC 1 cut(s) 1029
Hsp92II CATG 5 cut(s) 375, 599, 619, 631, 818
HspAI GCGC 1 cut(s) 402
Kpn2I TCCGGA 2 cut(s) 260, 700
Kzo9I GATC 5 cut(s) 158, 221, 725, 1044, 1081
LmnI GCTCC 1 cut(s) 835
Lsp1109I GCAGC 3 cut(s) 67, 775, 787
LweI GCATC 2 cut(s) 274, 942
MaeI CTAG 7 cut(s) 74, 165, 201, 309, 348, 578, 710
MaeIII GTNAC 2 cut(s) 383, 802
MalI GATC 5 cut(s) 160, 223, 727, 1046, 1083
MboI GATC 5 cut(s) 158, 221, 725, 1044, 1081
MflI RGATCY 3 cut(s) 158, 221, 1081
MluCI AATT 3 cut(s) 136, 567, 730
MmeI TCCRAC 1 cut(s) 927
MnlI CCTC 8 cut(s) 89, 172, 265, 445, 574, 669, 793, 826
MroI TCCGGA 2 cut(s) 260, 700
MseI TTAA 3 cut(s) 27, 135, 152
MspA1I CMGCKG 1 cut(s) 971
MspI CCGG 3 cut(s) 261, 701, 716
MwoI GCNNNNNNNGC 3 cut(s) 440, 553, 772
NdeII GATC 5 cut(s) 158, 221, 725, 1044, 1081
NlaIII CATG 5 cut(s) 375, 599, 619, 631, 818
NmuCI GTSAC 2 cut(s) 383, 802
PfeI GAWTC 4 cut(s) 368, 518, 809, 950
PflFI GACNNNGTC 1 cut(s) 547
PkrI GCNGC 3 cut(s) 57, 765, 777
PshBI ATTAAT 1 cut(s) 135
PspFI CCCAGC 1 cut(s) 917
PspPI GGNCC 1 cut(s) 173
PsuI RGATCY 3 cut(s) 158, 221, 1081
PsyI GACNNNGTC 1 cut(s) 547
PvuII CAGCTG 1 cut(s) 971
RsaI GTAC 2 cut(s) 576, 603
RsaNI GTAC 2 cut(s) 575, 602
SaqAI TTAA 3 cut(s) 27, 135, 152
SatI GCNGC 3 cut(s) 56, 764, 776
Sau3AI GATC 5 cut(s) 158, 221, 725, 1044, 1081
Sau96I GGNCC 1 cut(s) 173
ScaI AGTACT 1 cut(s) 576
SfaNI GCATC 2 cut(s) 274, 942
SinI GGWCC 1 cut(s) 173
SmlI CTYRAG 2 cut(s) 115, 459
SmoI CTYRAG 2 cut(s) 115, 459
Sse9I AATT 3 cut(s) 136, 567, 730
SspMI CTAG 7 cut(s) 74, 165, 201, 309, 348, 578, 710
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 5 cut(s) 363, 409, 986, 1060, 1071
TaqI TCGA 6 cut(s) 245, 524, 728, 749, 786, 936
TasI AATT 3 cut(s) 136, 567, 730
TatI WGTACW 1 cut(s) 574
TfiI GAWTC 4 cut(s) 368, 518, 809, 950
Tru1I TTAA 3 cut(s) 27, 135, 152
Tru9I TTAA 3 cut(s) 27, 135, 152
TscAI CASTG 3 cut(s) 298, 412, 510
TseFI GTSAC 2 cut(s) 383, 802
TseI GCWGC 3 cut(s) 55, 763, 775
Tsp45I GTSAC 2 cut(s) 383, 802
TspDTI ATGAA 8 cut(s) 381, 408, 438, 444, 497, 842, 939, 1005
TspRI CASTG 3 cut(s) 298, 412, 510
Tth111I GACNNNGTC 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 173
VspI ATTAAT 1 cut(s) 135
XapI RAATTY 1 cut(s) 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XspI CTAG 7 cut(s) 74, 165, 201, 309, 348, 578, 710
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.