Rh6DG490700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013622
Physical Location & Seq
Forward (+)
65077021 .. 65078370
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG490700.1

Sequence Viewer

Length: 1107 bp
ATGGCTTCCACAACAACAAAACCAGTTAAACAACTTGGAGAGCTTCTCCAAGAGCAGCAAGAACCCTTTGTCCTAGATGTTTACCTATTTGAAAGAGGGTGTCTGAGAAAGAGCTTGAGTTCAAGCAATTGCACTAATTCAAGCAAGAGTTTAAAGAGATCATCTAGTTGTGGACCAATCAAGAGCAAAAGGGGTACTCCTAGCTTCTCCAAGATACTGAGATCTGTCTATAACAAGCTTGTTTCCAAAAATGGTGGCTCGAGAACTAAGAGTTCCGGAAAAGAAGAGGGAAAGTTTGATGCCAACACTGAGATTACTCTTCCTAGTGATTGCCTTGGAGATTTGGATGGATTTTCTTCTTCTAGTAGCAGAACACAGTATGAATCATGCTGTGATAGTGACAAAGATGAAGCTTGCGCCACTGTTTTGTTGCAGAATGAAGATGAAGCCTCGCTTGCAGTAGAAGGCACTTCTCAAGTTCCAAATCTCTGCAGTATGAAAGAAGAGACAAAGATAGCACTGCTCAATGGGGAATCATCAAAGAAGAAGAAGACGCAATCTAAGCAACACACCTCAATTTCAGTACTAGAAGATAGACCTTCTCATGCAGTACCCAACAAGGTCATGGAAAACTCCATGTTATCAGCTTCTCTTTGGGAACTACTTTTCCAGCCACCATTGGAGAGGCCAAGAGATACTTTTGGAGTTCCAGAAATGCTAGAGCCGGTAATCAGATCGAATTCTTCTCCACACAAATCGAAAAGGATGTTGCAGCAGACAAGGCAGCTTCTTTTCGATTGTGTGAGAGAGGTGACAGAATCCCATGCAAAGCAAATGAAAGAGGAGCAGAACAGCACAAAGTTTTTGGGGGCGGAAGAACTGGGAAAGCTGATTTCTGAGAAATTAAGGATTTGGGATAGACAGGCTGGGGATGAAACCAACATCGACTTTTTGTTGGATTCAGATATCCTGAGTTCAGCTGAAGAATGGAATTGTAATGAACAACAAGAGAGAGAGATTTGCTGCGAAATTGGGGACGCCATTTTAGAAGAGATCATCAAAGAAACTGTTGCTCTACCGTTTAGTTTAGATCCTGTTTCCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378

Protein Analysis

368

Amino Acids

41.0

Weight (kDa)

5.23

Isoelectric Point (pI)

64.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 251 - 355 4.1e-07 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013622)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g32690
malus_domestica MD08G1078600.v1.1 MD15G1065400.v1.1
prunus_persica Prupe.1G419500_v2.0.a1
pyrus_communis pycom08g06500 pycom15g06190
rosa_chinensis RchiOBHm_Chr6g0309981
rosa_laevigata RLG00000010519
rosa_multiflora Rmu_sc0000294.1_g000010
rosa_roxburghii Rroxscaffold_7G00158980
rosa_rugosa Rorug06G0376500 Rorug06G0376600
rosa_samantha Rh6AG490300 Rh6BG499800 Rh6CG504600 Rh6CG504700 Rh6DG490700
rosa_wichuraiana Rw6G042680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 275
AciI CCGC 1 cut(s) 872
AclWI GGATC 1 cut(s) 1085
AcsI RAATTY 1 cut(s) 739
AcuI CTGAAG 1 cut(s) 1002
AcyI GRCGYC 1 cut(s) 1038
AfaI GTAC 3 cut(s) 196, 585, 612
AgsI TTSAA 3 cut(s) 92, 123, 141
AloI GAACNNNNNNTCC 4 cut(s) 54, 86, 651, 683
AluBI AGCT 9 cut(s) 43, 114, 204, 238, 413, 647, 787, 889, 980
AluI AGCT 9 cut(s) 43, 114, 204, 238, 413, 647, 787, 889, 980
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 1 cut(s) 1085
Ama87I CYCGRG 1 cut(s) 259
Aor13HI TCCGGA 1 cut(s) 275
AoxI GGCC 1 cut(s) 686
ApeKI GCWGC 4 cut(s) 55, 772, 784, 1023
ApoI RAATTY 1 cut(s) 739
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 823
AvaI CYCGRG 1 cut(s) 259
AvaII GGWCC 1 cut(s) 173
BbsI GAAGAC 1 cut(s) 557
BbvI GCAGC 4 cut(s) 67, 784, 796, 1010
BccI CCATC 1 cut(s) 341
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 7 cut(s) 74, 165, 201, 324, 363, 587, 719
BfmI CTRYAG 1 cut(s) 490
BglII AGATCT 1 cut(s) 221
BisI GCNGC 4 cut(s) 56, 773, 785, 1024
BlsI GCNGC 4 cut(s) 57, 774, 786, 1025
BmcAI AGTACT 1 cut(s) 585
Bme18I GGWCC 1 cut(s) 173
BmeT110I CYCGRG 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 173
BmrI ACTGGG 1 cut(s) 890
BmsI GCATC 1 cut(s) 289
BmuI ACTGGG 1 cut(s) 890
BpiI GAAGAC 1 cut(s) 557
BplI GAGNNNNNCTC 2 cut(s) 30, 62
BpuEI CTTGAG 2 cut(s) 136, 459
BsaHI GRCGYC 1 cut(s) 1038
BsaJI CCNNGG 1 cut(s) 334
BsaWI WCCGGW 1 cut(s) 275
Bse118I RCCGGY 1 cut(s) 724
Bse1I ACTGG 2 cut(s) 23, 885
BseAI TCCGGA 1 cut(s) 275
BseDI CCNNGG 1 cut(s) 334
BseGI GGATG 3 cut(s) 352, 771, 937
BseMII CTCAG 5 cut(s) 95, 209, 300, 888, 962
BseNI ACTGG 2 cut(s) 23, 885
BseRI GAGGAG 1 cut(s) 857
BseXI GCAGC 4 cut(s) 67, 784, 796, 1010
BseYI CCCAGC 1 cut(s) 926
BshFI GGCC 1 cut(s) 688
BsiHKCI CYCGRG 1 cut(s) 259
BsiSI CCGG 2 cut(s) 276, 725
BslFI GGGAC 1 cut(s) 1049
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 1 cut(s) 1049
BsnI GGCC 1 cut(s) 688
BsoBI CYCGRG 1 cut(s) 259
Bsp13I TCCGGA 1 cut(s) 275
Bsp143I GATC 5 cut(s) 158, 221, 734, 1053, 1090
BspACI CCGC 1 cut(s) 872
BspANI GGCC 1 cut(s) 688
BspCNI CTCAG 5 cut(s) 96, 210, 301, 889, 963
BspEI TCCGGA 1 cut(s) 275
BspMAI CTGCAG 1 cut(s) 494
BspPI GGATC 1 cut(s) 1085
BsrFI RCCGGY 1 cut(s) 724
BsrI ACTGG 2 cut(s) 23, 885
BssAI RCCGGY 1 cut(s) 724
BssECI CCNNGG 1 cut(s) 334
BssMI GATC 5 cut(s) 158, 221, 734, 1053, 1090
BssNI GRCGYC 1 cut(s) 1038
BssT1I CCWWGG 1 cut(s) 334
Bst4CI ACNGT 4 cut(s) 378, 424, 1069, 1080
Bst6I CTCTTC 4 cut(s) 279, 324, 498, 1044
BstACI GRCGYC 1 cut(s) 1038
BstC8I GCNNGC 2 cut(s) 415, 456
BstDEI CTNAG 7 cut(s) 104, 218, 267, 309, 561, 897, 971
BstF5I GGATG 3 cut(s) 352, 771, 937
BstHHI GCGC 1 cut(s) 419
BstKTI GATC 5 cut(s) 161, 224, 737, 1056, 1093
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 5 cut(s) 158, 221, 734, 1053, 1090
BstMWI GCNNNNNNNGC 3 cut(s) 455, 562, 781
BstSFI CTRYAG 1 cut(s) 490
BstV1I GCAGC 4 cut(s) 67, 784, 796, 1010
BstV2I GAAGAC 1 cut(s) 557
BstX2I RGATCY 2 cut(s) 221, 1090
BstYI RGATCY 2 cut(s) 221, 1090
BsuRI GGCC 1 cut(s) 688
BtsCI GGATG 3 cut(s) 352, 771, 937
BtsI GCAGTG 1 cut(s) 518
BtsIMutI CAGTG 3 cut(s) 306, 420, 518
Cac8I GCNNGC 2 cut(s) 415, 456
CfoI GCGC 1 cut(s) 419
Cfr10I RCCGGY 1 cut(s) 724
Cfr13I GGNCC 1 cut(s) 173
CseI GACGC 2 cut(s) 562, 1046
Csp6I GTAC 3 cut(s) 195, 584, 611
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 5 cut(s) 387, 605, 625, 637, 824
CviQI GTAC 3 cut(s) 195, 584, 611
DdeI CTNAG 7 cut(s) 104, 218, 267, 309, 561, 897, 971
DpnI GATC 5 cut(s) 160, 223, 736, 1055, 1092
DpnII GATC 5 cut(s) 158, 221, 734, 1053, 1090
DraI TTTAAA 1 cut(s) 153
Eam1104I CTCTTC 4 cut(s) 279, 324, 498, 1044
EarI CTCTTC 4 cut(s) 279, 324, 498, 1044
EciI GGCGGA 1 cut(s) 887
Eco130I CCWWGG 1 cut(s) 334
Eco32I GATATC 1 cut(s) 967
Eco47I GGWCC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 1002
Eco88I CYCGRG 1 cut(s) 259
EcoRI GAATTC 1 cut(s) 739
EcoRV GATATC 1 cut(s) 967
EcoT14I CCWWGG 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 334
FaeI CATG 5 cut(s) 390, 608, 628, 640, 827
FaiI YATR 8 cut(s) 231, 381, 388, 497, 606, 626, 638, 825
FalI AAGNNNNNCTT 4 cut(s) 438, 470, 682, 714
FaqI GGGAC 1 cut(s) 1049
FatI CATG 5 cut(s) 386, 604, 624, 636, 823
Fnu4HI GCNGC 4 cut(s) 56, 773, 785, 1024
FokI GGATG 3 cut(s) 359, 778, 944
Fsp4HI GCNGC 4 cut(s) 56, 773, 785, 1024
FspBI CTAG 7 cut(s) 74, 165, 201, 324, 363, 587, 719
GlaI GCGC 1 cut(s) 418
GluI GCNGC 4 cut(s) 56, 773, 785, 1024
GsaI CCCAGC 1 cut(s) 930
HaeIII GGCC 1 cut(s) 688
HapII CCGG 2 cut(s) 276, 725
HgaI GACGC 2 cut(s) 562, 1046
HhaI GCGC 1 cut(s) 419
Hin1I GRCGYC 1 cut(s) 1038
Hin1II CATG 5 cut(s) 390, 608, 628, 640, 827
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HindIII AAGCTT 2 cut(s) 236, 411
HinfI GANTC 4 cut(s) 383, 533, 818, 959
HpaII CCGG 2 cut(s) 276, 725
HphI GGTGA 1 cut(s) 823
Hpy166II GTNNAC 2 cut(s) 82, 173
Hpy188I TCNGA 5 cut(s) 105, 734, 898, 964, 1106
Hpy188III TCNNGA 5 cut(s) 181, 261, 276, 710, 970
Hpy8I GTNNAC 2 cut(s) 82, 173
HpyAV CCTTC 2 cut(s) 458, 609
HpyCH4III ACNGT 4 cut(s) 378, 424, 1069, 1080
HpyCH4V TGCA 7 cut(s) 132, 433, 458, 492, 608, 772, 827
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 562, 781
HpyF3I CTNAG 7 cut(s) 104, 218, 267, 309, 561, 897, 971
Hsp92I GRCGYC 1 cut(s) 1038
Hsp92II CATG 5 cut(s) 390, 608, 628, 640, 827
HspAI GCGC 1 cut(s) 417
Kpn2I TCCGGA 1 cut(s) 275
Kzo9I GATC 5 cut(s) 158, 221, 734, 1053, 1090
LmnI GCTCC 1 cut(s) 844
LpnPI CCDG 9 cut(s) 36, 289, 683, 723, 738, 866, 908, 912, 983
Lsp1109I GCAGC 4 cut(s) 67, 784, 796, 1010
LweI GCATC 1 cut(s) 289
MaeI CTAG 7 cut(s) 74, 165, 201, 324, 363, 587, 719
MaeIII GTNAC 2 cut(s) 398, 811
MalI GATC 5 cut(s) 160, 223, 736, 1055, 1092
MboI GATC 5 cut(s) 158, 221, 734, 1053, 1090
MfeI CAATTG 1 cut(s) 127
MflI RGATCY 2 cut(s) 221, 1090
MluCI AATT 7 cut(s) 127, 136, 576, 739, 902, 991, 1029
MmeI TCCRAC 1 cut(s) 936
MnlI CCTC 7 cut(s) 89, 280, 460, 583, 678, 802, 835
MroI TCCGGA 1 cut(s) 275
MseI TTAA 3 cut(s) 27, 152, 905
MspA1I CMGCKG 1 cut(s) 980
MspI CCGG 2 cut(s) 276, 725
MunI CAATTG 1 cut(s) 127
MwoI GCNNNNNNNGC 3 cut(s) 455, 562, 781
NdeII GATC 5 cut(s) 158, 221, 734, 1053, 1090
NlaIII CATG 5 cut(s) 390, 608, 628, 640, 827
NmuCI GTSAC 2 cut(s) 398, 811
PaeR7I CTCGAG 1 cut(s) 259
PfeI GAWTC 4 cut(s) 383, 533, 818, 959
PkrI GCNGC 4 cut(s) 57, 774, 786, 1025
PspFI CCCAGC 1 cut(s) 926
PspPI GGNCC 1 cut(s) 173
PstI CTGCAG 1 cut(s) 494
PsuI RGATCY 2 cut(s) 221, 1090
PvuII CAGCTG 1 cut(s) 980
RsaI GTAC 3 cut(s) 196, 585, 612
RsaNI GTAC 3 cut(s) 195, 584, 611
SaqAI TTAA 3 cut(s) 27, 152, 905
SatI GCNGC 4 cut(s) 56, 773, 785, 1024
Sau3AI GATC 5 cut(s) 158, 221, 734, 1053, 1090
Sau96I GGNCC 1 cut(s) 173
ScaI AGTACT 1 cut(s) 585
SfaNI GCATC 1 cut(s) 289
SfcI CTRYAG 1 cut(s) 490
Sfr274I CTCGAG 1 cut(s) 259
SinI GGWCC 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 259
SmlI CTYRAG 3 cut(s) 115, 259, 474
SmoI CTYRAG 3 cut(s) 115, 259, 474
Sse9I AATT 7 cut(s) 127, 136, 576, 739, 902, 991, 1029
SsiI CCGC 1 cut(s) 872
SspMI CTAG 7 cut(s) 74, 165, 201, 324, 363, 587, 719
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 4 cut(s) 378, 424, 1069, 1080
TaqI TCGA 5 cut(s) 260, 737, 758, 795, 945
TasI AATT 7 cut(s) 127, 136, 576, 739, 902, 991, 1029
TatI WGTACW 1 cut(s) 583
TfiI GAWTC 4 cut(s) 383, 533, 818, 959
Tru1I TTAA 3 cut(s) 27, 152, 905
Tru9I TTAA 3 cut(s) 27, 152, 905
TscAI CASTG 3 cut(s) 313, 427, 525
TseFI GTSAC 2 cut(s) 398, 811
TseI GCWGC 4 cut(s) 55, 772, 784, 1023
Tsp45I GTSAC 2 cut(s) 398, 811
TspDTI ATGAA 8 cut(s) 396, 423, 453, 459, 512, 851, 948, 1014
TspRI CASTG 3 cut(s) 313, 427, 525
VpaK11BI GGWCC 1 cut(s) 173
XapI RAATTY 1 cut(s) 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 622
XhoI CTCGAG 1 cut(s) 259
XspI CTAG 7 cut(s) 74, 165, 201, 324, 363, 587, 719
ZrmI AGTACT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.