Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0009047

Physical Location & Seq

Genomic Coordinates Forward (+)

7968389 .. 7970451

Gene Structure 2063 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1094900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGTGCGTGGAACTAATGATGGGATTGGTCGACAGTTTTGCAGCGAGGATGGAGGAGAATGCGGTGGAGGAGGCGGCCTCCGCCGGAATCCAGAGCGTCGAGAAGTTCATTAGCCTGATTTCGGAACACCACCAATCAACGTCGTCGAACTCGGAAGCTGGTACGGAGTATAAAGCCGTGGCGGACATGGCCGTGACCAAGTTCAGAAAGGTCATTTCGTTGTTGGACAAAGGAAGTACCGGCCACGCCCGGTTTCGGAAAGCTCCAGTGACTCTATCTCCGCCTCCCCCGCCGCCGGTGCCTCCGAAGACGATAACCCAAATTCTGAGACCTTCAATTTCAGAACCCCATGATCAAAAAACAGAGCAACCCTCTGTTTTCAGAACAGAACAATCTTCTGCTTTTAAGGTTTATTGTCCGAAGCCGGTGGTTCGCCTGCCGCCGCTGCCGCAAAATCCCCATCTGAAAACCACTCCGGTTGTGCTGACAAACGGTATTGGGTGCTCTGAGAGAAAGATGGATTCTGCTGCTGCCACCACCATCAACTTCTCGCCTTCGGCGCCCATTTCTGTCGCAAATTCGTTCATTTCGTCTTTAACTACTGGGGACCCCGCTGAGCAATCGATCTCTTCTGGGTTTCAATTTACAAATATGTCACAGTCATCTTCCGGTAGGCCGCCTCTGTCTTCGTCATCGTTGAAAAGAAAGTGCAGCTCCGTCGACAATGTTTCTCGTCTCCGGTGCGGATCTACTACCGGCCGCTGCCACTGCTCTAAGAAAAGGAAATCTAAAGTGAAAAGAGTGATTAGGGTTCCTGCAATTAGTATGAAACTGGCTGATATTCCTCCTGATGATTATTCTTGGAGAAAGTACGGTCAAAAACCCATCAAGGGTTCCCCTCATCCAAGAGGGTATTACAAGTGTAGCAGCCAGAGAGGTTGCCTTGCACGCAAGCACGTGGAGCGCGCTGTAGACGATCCCACCATGCTGATCGTGACCTACGAAGGCGATCACGATCACTCCCTCGGCGTCACCGAACCAACGGCCGCCCTCGTCCTCGAATCCTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Plant_zn_clust WRKY

Protein Analysis

357

Amino Acids

38.64

Weight (kDa)

9.41

Isoelectric Point (pI)

50.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Plant_zn_clust	PF10533	234 - 280	1.4e-16	Plant zinc cluster domain
WRKY	PF03106	284 - 340	4.9e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009047)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G24240 AT4G24240
fragaria_vesca	FvH4_2g33920 FvH4_2g33920 FvH4_2g33920 FvH4_2g33920
malus_domestica	MD08G1094900.v1.1 MD15G1078200.v1.1
prunus_persica	Prupe.1G431100_v2.0.a1 Prupe.1G431100_v2.0.a1
pyrus_communis	pycom08g07690 pycom15g07300
rosa_chinensis	RchiOBHm_Chr6g0308491
rosa_laevigata	RLG00000010638
rosa_roxburghii	Rroxscaffold_7G00160080
rosa_rugosa	Rorug06G0367200
rosa_samantha	Rh5DG522200 Rh6AG477100 Rh6BG487300 Rh6CG492300 Rh6DG478400
rosa_wichuraiana	Rw6G041610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	298, 559
AccI	GTMKAC	3 cut(s)	30, 720, 972
AccII	CGCG	1 cut(s)	966
AclWI	GGATC	2 cut(s)	754, 971
AcoI	YGGCCR	4 cut(s)	189, 241, 757, 1044
AcsI	RAATTY	2 cut(s)	321, 577
AcvI	CACGTG	1 cut(s)	958
AcyI	GRCGYC	2 cut(s)	560, 1029
AfaI	GTAC	3 cut(s)	163, 238, 872
AfiI	CCNNNNNNNGG	4 cut(s)	121, 255, 295, 890
AgsI	TTSAA	3 cut(s)	336, 641, 700
AluBI	AGCT	3 cut(s)	158, 263, 714
AluI	AGCT	3 cut(s)	158, 263, 714
Alw21I	GWGCWC	1 cut(s)	506
Alw26I	GTCTC	2 cut(s)	322, 740
AlwI	GGATC	2 cut(s)	754, 971
AoxI	GGCC	6 cut(s)	75, 189, 241, 674, 757, 1044
ApeKI	GCWGC	7 cut(s)	41, 445, 527, 530, 711, 762, 927
ApoI	RAATTY	2 cut(s)	321, 577
AspLEI	GCGC	3 cut(s)	562, 966, 968
AspS9I	GGNCC	1 cut(s)	607
AsuC2I	CCSGG	1 cut(s)	250
AsuHPI	GGTGA	1 cut(s)	1024
AvaII	GGWCC	1 cut(s)	607
BanI	GGYRCC	2 cut(s)	298, 559
BbrPI	CACGTG	1 cut(s)	958
BbsI	GAAGAC	2 cut(s)	314, 678
Bbv12I	GWGCWC	1 cut(s)	506
BbvI	GCAGC	7 cut(s)	53, 432, 514, 517, 723, 749, 939
BccI	CCATC	6 cut(s)	13, 43, 468, 511, 548, 893
BceAI	ACGGC	3 cut(s)	161, 176, 1059
BcgI	CGANNNNNNTGC	4 cut(s)	20, 54, 700, 734
BclI	TGATCA	1 cut(s)	352
BcnI	CCSGG	1 cut(s)	250
BcoDI	GTCTC	2 cut(s)	322, 740
BfmI	CTRYAG	1 cut(s)	969
BfoI	RGCGCY	1 cut(s)	563
BlpI	GCTNAGC	1 cut(s)	615
Bme1390I	CCNGG	1 cut(s)	250
Bme18I	GGWCC	1 cut(s)	607
BmgT120I	GGNCC	1 cut(s)	607
BmiI	GGNNCC	6 cut(s)	300, 561, 608, 609, 813, 895
BmrFI	CCNGG	1 cut(s)	250
BmrI	ACTGGG	1 cut(s)	612
BmuI	ACTGGG	1 cut(s)	612
BpiI	GAAGAC	2 cut(s)	314, 678
BplI	GAGNNNNNCTC	4 cut(s)	62, 94, 356, 388
BpmI	CTGGAG	1 cut(s)	249
Bpu1102I	GCTNAGC	1 cut(s)	615
BpuMI	CCSGG	1 cut(s)	250
Bsa29I	ATCGAT	1 cut(s)	623
BsaAI	YACGTR	1 cut(s)	958
BsaBI	GATNNNNATC	1 cut(s)	1014
BsaHI	GRCGYC	2 cut(s)	560, 1029
BsaI	GGTCTC	1 cut(s)	322
BsaJI	CCNNGG	2 cut(s)	177, 1024
BsaWI	WCCGGW	3 cut(s)	475, 668, 738
BsaXI	ACNNNNNCTCC	4 cut(s)	262, 292, 1004, 1034
Bsc4I	CCNNNNNNNGG	4 cut(s)	121, 255, 295, 890
Bse118I	RCCGGY	4 cut(s)	239, 295, 424, 755
Bse1I	ACTGG	3 cut(s)	266, 607, 837
Bse8I	GATNNNNATC	1 cut(s)	1014
BseCI	ATCGAT	1 cut(s)	623
BseDI	CCNNGG	2 cut(s)	177, 1024
BseGI	GGATG	2 cut(s)	54, 901
BseJI	GATNNNNATC	1 cut(s)	1014
BseLI	CCNNNNNNNGG	4 cut(s)	121, 255, 295, 890
BseMII	CTCAG	3 cut(s)	317, 498, 606
BseNI	ACTGG	3 cut(s)	266, 607, 837
BsePI	GCGCGC	1 cut(s)	964
BseRI	GAGGAG	2 cut(s)	68, 83
BseX3I	CGGCCG	2 cut(s)	757, 1044
BseXI	GCAGC	7 cut(s)	53, 432, 514, 517, 723, 749, 939
BsgI	GTGCAG	1 cut(s)	730
Bsh1236I	CGCG	1 cut(s)	966
Bsh1285I	CGRYCG	2 cut(s)	760, 1047
BshFI	GGCC	6 cut(s)	77, 191, 243, 676, 759, 1046
BshNI	GGYRCC	2 cut(s)	298, 559
BshVI	ATCGAT	1 cut(s)	623
BsiEI	CGRYCG	2 cut(s)	760, 1047
BsiHKAI	GWGCWC	1 cut(s)	506
BsiSI	CCGG	9 cut(s)	84, 240, 250, 296, 425, 476, 669, 739, 756
BslFI	GGGAC	1 cut(s)	620
BslI	CCNNNNNNNGG	4 cut(s)	121, 255, 295, 890
BsmAI	GTCTC	2 cut(s)	322, 740
BsmBI	CGTCTC	1 cut(s)	740
BsmFI	GGGAC	1 cut(s)	620
BsmI	GAATGC	1 cut(s)	64
BsnI	GGCC	6 cut(s)	77, 191, 243, 676, 759, 1046
Bso31I	GGTCTC	1 cut(s)	322
Bsp1286I	GDGCHC	1 cut(s)	506
Bsp143I	GATC	7 cut(s)	352, 624, 746, 976, 990, 1009, 1015
Bsp1720I	GCTNAGC	1 cut(s)	615
BspANI	GGCC	6 cut(s)	77, 191, 243, 676, 759, 1046
BspCNI	CTCAG	3 cut(s)	318, 499, 607
BspDI	ATCGAT	1 cut(s)	623
BspFNI	CGCG	1 cut(s)	966
BspLI	GGNNCC	6 cut(s)	300, 561, 608, 609, 813, 895
BspPI	GGATC	2 cut(s)	754, 971
BspT107I	GGYRCC	2 cut(s)	298, 559
BspTNI	GGTCTC	1 cut(s)	322
BsrFI	RCCGGY	4 cut(s)	239, 295, 424, 755
BsrI	ACTGG	3 cut(s)	266, 607, 837
BssAI	RCCGGY	4 cut(s)	239, 295, 424, 755
BssECI	CCNNGG	2 cut(s)	177, 1024
BssHII	GCGCGC	1 cut(s)	964
BssMI	GATC	7 cut(s)	352, 624, 746, 976, 990, 1009, 1015
BssNI	GRCGYC	2 cut(s)	560, 1029
Bst4CI	ACNGT	4 cut(s)	35, 494, 660, 875
Bst6I	CTCTTC	1 cut(s)	634
BstACI	GRCGYC	2 cut(s)	560, 1029
BstBAI	YACGTR	1 cut(s)	958
BstC8I	GCNNGC	4 cut(s)	437, 949, 953, 966
BstDEI	CTNAG	4 cut(s)	326, 507, 615, 774
BstDSI	CCRYGG	1 cut(s)	177
BstF5I	GGATG	2 cut(s)	54, 901
BstFNI	CGCG	1 cut(s)	966
BstH2I	RGCGCY	1 cut(s)	563
BstHHI	GCGC	3 cut(s)	562, 966, 968
BstKTI	GATC	7 cut(s)	355, 627, 749, 979, 993, 1012, 1018
BstMAI	GTCTC	2 cut(s)	322, 740
BstMBI	GATC	7 cut(s)	352, 624, 746, 976, 990, 1009, 1015
BstMCI	CGRYCG	2 cut(s)	760, 1047
BstSCI	CCNGG	1 cut(s)	248
BstSFI	CTRYAG	1 cut(s)	969
BstUI	CGCG	1 cut(s)	966
BstV1I	GCAGC	7 cut(s)	53, 432, 514, 517, 723, 749, 939
BstV2I	GAAGAC	2 cut(s)	314, 678
BstX2I	RGATCY	1 cut(s)	746
BstYI	RGATCY	1 cut(s)	746
BstZI	CGGCCG	2 cut(s)	757, 1044
Bsu15I	ATCGAT	1 cut(s)	623
BsuRI	GGCC	6 cut(s)	77, 191, 243, 676, 759, 1046
BsuTUI	ATCGAT	1 cut(s)	623
BtgI	CCRYGG	1 cut(s)	177
BtsCI	GGATG	2 cut(s)	54, 901
BtsI	GCAGTG	1 cut(s)	766
BtsIMutI	CAGTG	2 cut(s)	273, 766
Cac8I	GCNNGC	4 cut(s)	437, 949, 953, 966
CfoI	GCGC	3 cut(s)	562, 966, 968
Cfr10I	RCCGGY	4 cut(s)	239, 295, 424, 755
Cfr13I	GGNCC	1 cut(s)	607
ClaI	ATCGAT	1 cut(s)	623
CseI	GACGC	2 cut(s)	85, 1018
Csp6I	GTAC	3 cut(s)	162, 237, 871
CspCI	CAANNNNNGTGG	2 cut(s)	460, 495
CviAII	CATG	3 cut(s)	187, 350, 985
CviQI	GTAC	3 cut(s)	162, 237, 871
DdeI	CTNAG	4 cut(s)	326, 507, 615, 774
DinI	GGCGCC	1 cut(s)	561
DpnI	GATC	7 cut(s)	354, 626, 748, 978, 992, 1011, 1017
DpnII	GATC	7 cut(s)	352, 624, 746, 976, 990, 1009, 1015
EaeI	YGGCCR	4 cut(s)	189, 241, 757, 1044
EagI	CGGCCG	2 cut(s)	757, 1044
Eam1104I	CTCTTC	1 cut(s)	634
EarI	CTCTTC	1 cut(s)	634
EciI	GGCGGA	3 cut(s)	70, 197, 270
EclXI	CGGCCG	2 cut(s)	757, 1044
Eco31I	GGTCTC	1 cut(s)	322
Eco47I	GGWCC	1 cut(s)	607
Eco52I	CGGCCG	2 cut(s)	757, 1044
Eco72I	CACGTG	1 cut(s)	958
EcoO109I	RGGNCCY	1 cut(s)	607
EgeI	GGCGCC	1 cut(s)	561
EheI	GGCGCC	1 cut(s)	561
Esp3I	CGTCTC	1 cut(s)	740
FaeI	CATG	3 cut(s)	190, 353, 988
FaiI	YATR	6 cut(s)	171, 188, 351, 653, 827, 986
FaqI	GGGAC	1 cut(s)	620
FatI	CATG	3 cut(s)	186, 349, 984
FauI	CCCGC	2 cut(s)	297, 619
FbaI	TGATCA	1 cut(s)	352
FblI	GTMKAC	3 cut(s)	30, 720, 972
FokI	GGATG	2 cut(s)	61, 888
GlaI	GCGC	3 cut(s)	561, 965, 967
GsuI	CTGGAG	1 cut(s)	249
HaeII	RGCGCY	1 cut(s)	563
HaeIII	GGCC	6 cut(s)	77, 191, 243, 676, 759, 1046
HapII	CCGG	9 cut(s)	84, 240, 250, 296, 425, 476, 669, 739, 756
HgaI	GACGC	2 cut(s)	85, 1018
HhaI	GCGC	3 cut(s)	562, 966, 968
Hin1I	GRCGYC	2 cut(s)	560, 1029
Hin1II	CATG	3 cut(s)	190, 353, 988
Hin6I	GCGC	3 cut(s)	560, 964, 966
HinP1I	GCGC	3 cut(s)	560, 964, 966
HincII	GTYRAC	2 cut(s)	31, 721
HindII	GTYRAC	2 cut(s)	31, 721
HinfI	GANTC	4 cut(s)	87, 271, 521, 1061
HpaII	CCGG	9 cut(s)	84, 240, 250, 296, 425, 476, 669, 739, 756
HphI	GGTGA	1 cut(s)	1024
Hpy166II	GTNNAC	3 cut(s)	31, 721, 973
Hpy188III	TCNNGA	5 cut(s)	91, 100, 848, 994, 1013
Hpy8I	GTNNAC	3 cut(s)	31, 721, 973
Hpy99I	CGWCG	4 cut(s)	101, 145, 148, 722
HpyAV	CCTTC	3 cut(s)	342, 564, 998
HpyCH4III	ACNGT	4 cut(s)	35, 494, 660, 875
HpyCH4IV	ACGT	2 cut(s)	140, 957
HpyCH4V	TGCA	4 cut(s)	41, 711, 818, 947
HpyF3I	CTNAG	4 cut(s)	326, 507, 615, 774
HpySE526I	ACGT	2 cut(s)	140, 957
Hsp92I	GRCGYC	2 cut(s)	560, 1029
Hsp92II	CATG	3 cut(s)	190, 353, 988
HspAI	GCGC	3 cut(s)	560, 964, 966
KasI	GGCGCC	1 cut(s)	559
KflI	GGGWCCC	1 cut(s)	607
Ksp22I	TGATCA	1 cut(s)	352
Kzo9I	GATC	7 cut(s)	352, 624, 746, 976, 990, 1009, 1015
LmnI	GCTCC	3 cut(s)	268, 719, 961
Lsp1109I	GCAGC	7 cut(s)	53, 432, 514, 517, 723, 749, 939
MaeII	ACGT	2 cut(s)	140, 957
MaeIII	GTNAC	5 cut(s)	193, 268, 654, 994, 1030
MalI	GATC	7 cut(s)	354, 626, 748, 978, 992, 1011, 1017
MboI	GATC	7 cut(s)	352, 624, 746, 976, 990, 1009, 1015
MboII	GAAGA	5 cut(s)	319, 387, 621, 657, 678
MflI	RGATCY	1 cut(s)	746
MhlI	GDGCHC	1 cut(s)	506
MluCI	AATT	5 cut(s)	321, 336, 577, 641, 819
Mly113I	GGCGCC	1 cut(s)	560
MlyI	GAGTC	1 cut(s)	265
MmeI	TCCRAC	1 cut(s)	204
MseI	TTAA	3 cut(s)	405, 596, 1069
MslI	CAYNNNNRTG	1 cut(s)	191
MspA1I	CMGCKG	3 cut(s)	445, 614, 762
MspI	CCGG	9 cut(s)	84, 240, 250, 296, 425, 476, 669, 739, 756
MspR9I	CCNGG	1 cut(s)	250
Mva1269I	GAATGC	1 cut(s)	64
MvnI	CGCG	1 cut(s)	966
NarI	GGCGCC	1 cut(s)	560
NciI	CCSGG	1 cut(s)	250
NdeII	GATC	7 cut(s)	352, 624, 746, 976, 990, 1009, 1015
NlaIII	CATG	3 cut(s)	190, 353, 988
NlaIV	GGNNCC	6 cut(s)	300, 561, 608, 609, 813, 895
NmeAIII	GCCGAG	1 cut(s)	1005
NmuCI	GTSAC	5 cut(s)	193, 268, 654, 994, 1030
PauI	GCGCGC	1 cut(s)	964
PcsI	WCGNNNNNNNCGW	3 cut(s)	587, 999, 1032
PctI	GAATGC	1 cut(s)	64
PfeI	GAWTC	3 cut(s)	87, 521, 1061
PleI	GAGTC	1 cut(s)	265
PluTI	GGCGCC	1 cut(s)	563
PmaCI	CACGTG	1 cut(s)	958
PmlI	CACGTG	1 cut(s)	958
PpsI	GAGTC	1 cut(s)	265
Ppu21I	YACGTR	1 cut(s)	958
PpuMI	RGGWCCY	1 cut(s)	607
Psp5II	RGGWCCY	1 cut(s)	607
PspCI	CACGTG	1 cut(s)	958
PspN4I	GGNNCC	6 cut(s)	300, 561, 608, 609, 813, 895
PspPI	GGNCC	1 cut(s)	607
PspPPI	RGGWCCY	1 cut(s)	607
PsuI	RGATCY	1 cut(s)	746
PteI	GCGCGC	1 cut(s)	964
RsaI	GTAC	3 cut(s)	163, 238, 872
RsaNI	GTAC	3 cut(s)	162, 237, 871
RseI	CAYNNNNRTG	1 cut(s)	191
SalI	GTCGAC	2 cut(s)	29, 719
SaqAI	TTAA	3 cut(s)	405, 596, 1069
Sau3AI	GATC	7 cut(s)	352, 624, 746, 976, 990, 1009, 1015
Sau96I	GGNCC	1 cut(s)	607
SchI	GAGTC	1 cut(s)	265
ScrFI	CCNGG	1 cut(s)	250
SduI	GDGCHC	1 cut(s)	506
SfcI	CTRYAG	1 cut(s)	969
SfoI	GGCGCC	1 cut(s)	561
SgrAI	CRCCGGYG	1 cut(s)	295
SinI	GGWCC	1 cut(s)	607
SmiMI	CAYNNNNRTG	1 cut(s)	191
Sse9I	AATT	5 cut(s)	321, 336, 577, 641, 819
SspDI	GGCGCC	1 cut(s)	559
StyD4I	CCNGG	1 cut(s)	248
TaaI	ACNGT	4 cut(s)	35, 494, 660, 875
TaiI	ACGT	2 cut(s)	143, 960
TaqI	TCGA	6 cut(s)	30, 99, 146, 623, 720, 1059
TasI	AATT	5 cut(s)	321, 336, 577, 641, 819
TauI	GCSGC	8 cut(s)	77, 295, 442, 445, 451, 679, 762, 1049
TfiI	GAWTC	3 cut(s)	87, 521, 1061
Tru1I	TTAA	3 cut(s)	405, 596, 1069
Tru9I	TTAA	3 cut(s)	405, 596, 1069
TscAI	CASTG	2 cut(s)	273, 773
TseFI	GTSAC	5 cut(s)	193, 268, 654, 994, 1030
TseI	GCWGC	7 cut(s)	41, 445, 527, 530, 711, 762, 927
Tsp45I	GTSAC	5 cut(s)	193, 268, 654, 994, 1030
TspDTI	ATGAA	3 cut(s)	97, 574, 842
TspGWI	ACGGA	2 cut(s)	179, 706
TspRI	CASTG	2 cut(s)	273, 773
VpaK11BI	GGWCC	1 cut(s)	607
XapI	RAATTY	2 cut(s)	321, 577
XmiI	GTMKAC	3 cut(s)	30, 720, 972

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.