MD08G1124000.v1.1

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006199
Physical Location & Seq
Forward (+)
11654816 .. 11655337
522 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1124000.v1.1.491

Sequence Viewer

Length: 522 bp
ATGAACACCACCATGGATTCAAAGAAATCTAACAAGATCAGAGACATTGTCAGGCTCCGACAGATTCTCAAGAAGTGGAGAAAGATTGCTAACTCATCAAATGCAATCTCCGGCACCACCGCCGCCACCACTGCAACTAGCAGCAGCAAGAGCATCAAGTTTTTGAAGAGGACACTTTCTCTGCCTGAAAAAACAACGACTTCATTTGAAGCATCAAACAATGCTGCTGTCCCCAAAGGCTACCTTGCTGTCTGTGTTGGGGAAGAGCTCAAGAGGTTTGTCATCCCAACTGACTACTTGGGTCGGCCGGCTTTCCACATTCTGTTGAGGGAGGCGGAGGAGGAGTTCGGGTTTCAGCAGACCGGCGTTCTGAGGATTCCATGTGAGGTTTCTGTGTTTGAAGGTATTCTGAAGATGGTGGAGGAAGACAAGGACATGTTCTTCAATAATATGGGAGGCTGCTCATCATCTGAGGGCCAGCTCAACTTTTCTCACCACCCAGAAAGCCCAATGTGCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

174

Amino Acids

19.3

Weight (kDa)

9.1

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 19 - 140 7e-27 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 113
AciI CCGC 3 cut(s) 120, 123, 335
AcoI YGGCCR 1 cut(s) 305
AcuI CTGAAG 1 cut(s) 431
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 5 cut(s) 21, 166, 209, 401, 445
AloI GAACNNNNNNTCC 2 cut(s) 329, 361
AluBI AGCT 2 cut(s) 268, 481
AluI AGCT 2 cut(s) 268, 481
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 36
AoxI GGCC 2 cut(s) 305, 475
ApeKI GCWGC 4 cut(s) 141, 144, 224, 459
Asp700I GAANNNNTTC 1 cut(s) 405
AspS9I GGNCC 1 cut(s) 475
AsuHPI GGTGA 1 cut(s) 485
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 270
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 4 cut(s) 153, 156, 211, 446
BccI CCATC 1 cut(s) 409
BcoDI GTCTC 1 cut(s) 36
BfaI CTAG 1 cut(s) 138
BisI GCNGC 5 cut(s) 123, 142, 145, 225, 460
BlsI GCNGC 5 cut(s) 124, 143, 146, 226, 461
BmgT120I GGNCC 1 cut(s) 475
BmiI GGNNCC 2 cut(s) 56, 115
BmsI GCATC 2 cut(s) 162, 221
BpiI GAAGAC 1 cut(s) 432
BpuEI CTTGAG 2 cut(s) 53, 254
BsaJI CCNNGG 1 cut(s) 12
BsaXI ACNNNNNCTCC 4 cut(s) 329, 335, 359, 365
Bse118I RCCGGY 2 cut(s) 307, 362
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 1 cut(s) 282
BseMII CTCAG 2 cut(s) 362, 462
BseRI GAGGAG 2 cut(s) 353, 356
BseX3I CGGCCG 1 cut(s) 305
BseXI GCAGC 4 cut(s) 153, 156, 211, 446
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 2 cut(s) 307, 477
BshNI GGYRCC 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 3 cut(s) 111, 308, 363
BslFI GGGAC 1 cut(s) 215
BsmAI GTCTC 1 cut(s) 36
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 2 cut(s) 307, 477
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 1 cut(s) 36
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 3 cut(s) 120, 123, 335
BspANI GGCC 2 cut(s) 307, 477
BspCNI CTCAG 2 cut(s) 363, 463
BspLI GGNNCC 2 cut(s) 56, 115
BspQI GCTCTTC 1 cut(s) 258
BspT107I GGYRCC 1 cut(s) 113
BsrFI RCCGGY 2 cut(s) 307, 362
BssAI RCCGGY 2 cut(s) 307, 362
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 12
Bst6I CTCTTC 2 cut(s) 161, 258
BstC8I GCNNGC 2 cut(s) 309, 479
BstDEI CTNAG 2 cut(s) 371, 471
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 1 cut(s) 282
BstKTI GATC 1 cut(s) 39
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 1 cut(s) 36
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 3 cut(s) 131, 150, 513
BstNSI RCATGY 1 cut(s) 439
BstV1I GCAGC 4 cut(s) 153, 156, 211, 446
BstV2I GAAGAC 1 cut(s) 432
BstZI CGGCCG 1 cut(s) 305
BsuRI GGCC 2 cut(s) 307, 477
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 1 cut(s) 282
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 129
Cac8I GCNNGC 2 cut(s) 309, 479
Cfr10I RCCGGY 2 cut(s) 307, 362
Cfr13I GGNCC 1 cut(s) 475
CviAII CATG 3 cut(s) 13, 381, 436
CviJI RGCY 9 cut(s) 55, 240, 268, 307, 311, 459, 477, 481, 507
CviKI_1 RGCY 9 cut(s) 55, 240, 268, 307, 311, 459, 477, 481, 507
DdeI CTNAG 2 cut(s) 371, 471
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
EaeI YGGCCR 1 cut(s) 305
EagI CGGCCG 1 cut(s) 305
Eam1104I CTCTTC 2 cut(s) 161, 258
EarI CTCTTC 2 cut(s) 161, 258
EciI GGCGGA 1 cut(s) 350
Ecl136II GAGCTC 1 cut(s) 268
EclXI CGGCCG 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 270
Eco52I CGGCCG 1 cut(s) 305
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 431
EcoICRI GAGCTC 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 3 cut(s) 16, 384, 439
FaiI YATR 4 cut(s) 14, 382, 437, 452
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 1 cut(s) 215
FatI CATG 3 cut(s) 12, 380, 435
Fnu4HI GCNGC 5 cut(s) 123, 142, 145, 225, 460
FokI GGATG 1 cut(s) 269
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 5 cut(s) 123, 142, 145, 225, 460
FspBI CTAG 1 cut(s) 138
GluI GCNGC 5 cut(s) 123, 142, 145, 225, 460
HaeIII GGCC 2 cut(s) 307, 477
HapII CCGG 3 cut(s) 111, 308, 363
Hin1II CATG 3 cut(s) 16, 384, 439
HinfI GANTC 3 cut(s) 17, 64, 376
HpaII CCGG 3 cut(s) 111, 308, 363
HphI GGTGA 1 cut(s) 485
Hpy188I TCNGA 5 cut(s) 41, 59, 372, 411, 472
Hpy188III TCNNGA 2 cut(s) 70, 271
HpyAV CCTTC 1 cut(s) 395
HpyCH4V TGCA 3 cut(s) 104, 134, 516
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 150, 513
HpyF3I CTNAG 2 cut(s) 371, 471
Hsp92II CATG 3 cut(s) 16, 384, 439
KroI GCCGGC 1 cut(s) 307
KroNI GCCGGC 1 cut(s) 309
Kzo9I GATC 1 cut(s) 36
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 7 cut(s) 37, 124, 198, 321, 376, 491, 513
Lsp1109I GCAGC 4 cut(s) 153, 156, 211, 446
LweI GCATC 2 cut(s) 162, 221
MaeI CTAG 1 cut(s) 138
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MboII GAAGA 5 cut(s) 178, 275, 424, 433, 437
MhlI GDGCHC 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 82
MroNI GCCGGC 1 cut(s) 307
MroXI GAANNNNTTC 1 cut(s) 405
MslI CAYNNNNRTG 1 cut(s) 11
MspI CCGG 3 cut(s) 111, 308, 363
MwoI GCNNNNNNNGC 3 cut(s) 131, 150, 513
NaeI GCCGGC 1 cut(s) 309
NcoI CCATGG 1 cut(s) 12
NdeII GATC 1 cut(s) 36
NgoMIV GCCGGC 1 cut(s) 307
NlaIII CATG 3 cut(s) 16, 384, 439
NlaIV GGNNCC 2 cut(s) 56, 115
NspI RCATGY 1 cut(s) 439
PciI ACATGT 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 258
PdiI GCCGGC 1 cut(s) 309
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 3 cut(s) 17, 64, 376
PflFI GACNNNGTC 1 cut(s) 47
PkrI GCNGC 5 cut(s) 124, 143, 146, 226, 461
PscI ACATGT 1 cut(s) 435
Psp124BI GAGCTC 1 cut(s) 270
PspN4I GGNNCC 2 cut(s) 56, 115
PspPI GGNCC 1 cut(s) 475
PsyI GACNNNGTC 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 11
SacI GAGCTC 1 cut(s) 270
SapI GCTCTTC 1 cut(s) 258
SatI GCNGC 5 cut(s) 123, 142, 145, 225, 460
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 1 cut(s) 475
SduI GDGCHC 1 cut(s) 270
SetI ASST 6 cut(s) 246, 270, 278, 390, 406, 483
SfaNI GCATC 2 cut(s) 162, 221
SmiMI CAYNNNNRTG 1 cut(s) 11
SmlI CTYRAG 2 cut(s) 68, 269
SmoI CTYRAG 2 cut(s) 68, 269
SsiI CCGC 3 cut(s) 120, 123, 335
SspMI CTAG 1 cut(s) 138
SstI GAGCTC 1 cut(s) 270
StyI CCWWGG 1 cut(s) 12
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 3 cut(s) 17, 64, 376
TscAI CASTG 1 cut(s) 136
TseI GCWGC 4 cut(s) 141, 144, 224, 459
TspDTI ATGAA 2 cut(s) 17, 192
TspRI CASTG 1 cut(s) 136
Tth111I GACNNNGTC 1 cut(s) 47
XceI RCATGY 1 cut(s) 439
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.