Rroxscaffold_7G00162790

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006199
Physical Location & Seq
Forward (+)
5192960 .. 5193442
483 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00162790.1

Sequence Viewer

Length: 483 bp
ATGGATTCAAAGAAGTCTAACAAGATCAGGGAGATTGTGAGGCTTCAACAGATTCTCAAAAAGTGGAGAAAGATCGCCAACTCGCCAAAAGCGAGCTCGAATACCATCAGCAGCAGCAACAAGAGCATCAAGTTTCTGAAAAGAACACTTTCTCTATCAGACAAAACAGGCAGTACCTGTGAAGCTTCTAACAATGCTCATGTCCCCAAAGGCTACCTTGCTGTTTGTGTTGGAGAAGAGCTCAAGAGATTCATCATTCCAACAGATTATCTTGGCCGTCCTGCTTTTCAGTTTCTGCTGAGAGAAGCTGAGGAGGAGTTCGGGTTTCAACAGACCGGCGTTCTGAGGATTCCATGTGAGGTTTCTGTGTTCGAAGAGCTTCTGAAGATGGTGGAGGAAGATAGGGATACATTCTTCATGCAAGGATGCTATTCAGAAAGCCAGCTCAACTTTTACCACCCAGAAAGCCCAATGTGCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

160

Amino Acids

18.37

Weight (kDa)

8.8

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 14 - 131 1.5e-28 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 274
AcuI CTGAAG 1 cut(s) 404
AfaI GTAC 1 cut(s) 175
AgsI TTSAA 3 cut(s) 9, 47, 329
AluBI AGCT 6 cut(s) 96, 185, 241, 308, 379, 445
AluI AGCT 6 cut(s) 96, 185, 241, 308, 379, 445
Alw21I GWGCWC 2 cut(s) 98, 243
AlwNI CAGNNNCTG 2 cut(s) 177, 295
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 2 cut(s) 111, 114
Asp700I GAANNNNTTC 2 cut(s) 148, 378
AsuII TTCGAA 1 cut(s) 372
BanII GRGCYC 2 cut(s) 98, 243
Bbv12I GWGCWC 2 cut(s) 98, 243
BbvCI CCTCAGC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 123, 126
BccI CCATC 2 cut(s) 113, 382
BceAI ACGGC 1 cut(s) 261
BciVI GTATCC 1 cut(s) 400
BfuI GTATCC 1 cut(s) 400
BisI GCNGC 2 cut(s) 112, 115
BlsI GCNGC 2 cut(s) 113, 116
BmsI GCATC 2 cut(s) 135, 416
BplI GAGNNNNNCTC 2 cut(s) 225, 257
Bpu10I CCTNAGC 1 cut(s) 309
Bpu14I TTCGAA 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bse118I RCCGGY 1 cut(s) 335
BseGI GGATG 1 cut(s) 431
BseMII CTCAG 3 cut(s) 290, 300, 335
BseRI GAGGAG 2 cut(s) 326, 329
BseXI GCAGC 2 cut(s) 123, 126
BshFI GGCC 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 98, 243
BsiSI CCGG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 188
BsmFI GGGAC 1 cut(s) 188
BsnI GGCC 1 cut(s) 276
Bsp119I TTCGAA 1 cut(s) 372
Bsp1286I GDGCHC 2 cut(s) 98, 243
Bsp143I GATC 2 cut(s) 24, 72
BspANI GGCC 1 cut(s) 276
BspCNI CTCAG 3 cut(s) 291, 301, 336
BspQI GCTCTTC 2 cut(s) 231, 369
BspT104I TTCGAA 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 335
BssAI RCCGGY 1 cut(s) 335
BssMI GATC 2 cut(s) 24, 72
Bst6I CTCTTC 2 cut(s) 231, 369
BstBI TTCGAA 1 cut(s) 372
BstC8I GCNNGC 2 cut(s) 94, 443
BstDEI CTNAG 3 cut(s) 299, 309, 344
BstF5I GGATG 1 cut(s) 431
BstKTI GATC 2 cut(s) 27, 75
BstMBI GATC 2 cut(s) 24, 72
BstMWI GCNNNNNNNGC 2 cut(s) 123, 474
BstV1I GCAGC 2 cut(s) 123, 126
BsuI GTATCC 1 cut(s) 400
BsuRI GGCC 1 cut(s) 276
BtsCI GGATG 1 cut(s) 431
Cac8I GCNNGC 2 cut(s) 94, 443
CaiI CAGNNNCTG 2 cut(s) 177, 295
Cfr10I RCCGGY 1 cut(s) 335
Csp6I GTAC 1 cut(s) 174
CviAII CATG 3 cut(s) 200, 354, 418
CviQI GTAC 1 cut(s) 174
DdeI CTNAG 3 cut(s) 299, 309, 344
DpnI GATC 2 cut(s) 26, 74
DpnII GATC 2 cut(s) 24, 72
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 2 cut(s) 231, 369
EarI CTCTTC 2 cut(s) 231, 369
Ecl136II GAGCTC 2 cut(s) 96, 241
Eco24I GRGCYC 2 cut(s) 98, 243
Eco53kI GAGCTC 2 cut(s) 96, 241
Eco57I CTGAAG 1 cut(s) 404
EcoICRI GAGCTC 2 cut(s) 96, 241
EcoT38I GRGCYC 2 cut(s) 98, 243
FaeI CATG 3 cut(s) 203, 357, 421
FaiI YATR 3 cut(s) 201, 355, 419
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 1 cut(s) 188
FatI CATG 3 cut(s) 199, 353, 417
Fnu4HI GCNGC 2 cut(s) 112, 115
FokI GGATG 1 cut(s) 438
FriOI GRGCYC 2 cut(s) 98, 243
Fsp4HI GCNGC 2 cut(s) 112, 115
GluI GCNGC 2 cut(s) 112, 115
HaeIII GGCC 1 cut(s) 276
HapII CCGG 1 cut(s) 336
Hin1II CATG 3 cut(s) 203, 357, 421
HindIII AAGCTT 1 cut(s) 183
HinfI GANTC 4 cut(s) 5, 52, 249, 349
HpaII CCGG 1 cut(s) 336
Hpy188I TCNGA 5 cut(s) 138, 160, 345, 384, 436
Hpy188III TCNNGA 1 cut(s) 244
HpyCH4V TGCA 2 cut(s) 421, 477
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 474
HpyF3I CTNAG 3 cut(s) 299, 309, 344
Hsp92II CATG 3 cut(s) 203, 357, 421
Kzo9I GATC 2 cut(s) 24, 72
LguI GCTCTTC 2 cut(s) 231, 369
LpnPI CCDG 7 cut(s) 13, 153, 190, 294, 349, 455, 474
Lsp1109I GCAGC 2 cut(s) 123, 126
LweI GCATC 2 cut(s) 135, 416
MalI GATC 2 cut(s) 26, 74
MboI GATC 2 cut(s) 24, 72
MboII GAAGA 5 cut(s) 248, 386, 397, 406, 410
MhlI GDGCHC 2 cut(s) 98, 243
MmeI TCCRAC 2 cut(s) 211, 284
MnlI CCTC 6 cut(s) 33, 304, 307, 339, 352, 388
MroXI GAANNNNTTC 2 cut(s) 148, 378
MspI CCGG 1 cut(s) 336
MwoI GCNNNNNNNGC 2 cut(s) 123, 474
NdeII GATC 2 cut(s) 24, 72
NlaIII CATG 3 cut(s) 203, 357, 421
NspV TTCGAA 1 cut(s) 372
PciSI GCTCTTC 2 cut(s) 231, 369
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PdmI GAANNNNTTC 2 cut(s) 148, 378
PfeI GAWTC 4 cut(s) 5, 52, 249, 349
PkrI GCNGC 2 cut(s) 113, 116
Psp124BI GAGCTC 2 cut(s) 98, 243
PstNI CAGNNNCTG 2 cut(s) 177, 295
RsaI GTAC 1 cut(s) 175
RsaNI GTAC 1 cut(s) 174
SacI GAGCTC 2 cut(s) 98, 243
SapI GCTCTTC 2 cut(s) 231, 369
SatI GCNGC 2 cut(s) 112, 115
Sau3AI GATC 2 cut(s) 24, 72
SduI GDGCHC 2 cut(s) 98, 243
SetI ASST 9 cut(s) 98, 179, 187, 219, 243, 310, 363, 381, 447
SfaNI GCATC 2 cut(s) 135, 416
SfuI TTCGAA 1 cut(s) 372
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
SstI GAGCTC 2 cut(s) 98, 243
TaqI TCGA 2 cut(s) 98, 372
TfiI GAWTC 4 cut(s) 5, 52, 249, 349
TseI GCWGC 2 cut(s) 111, 114
TspDTI ATGAA 2 cut(s) 241, 406
XmnI GAANNNNTTC 2 cut(s) 148, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.