MD17G1161400.v1.1

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006199
Physical Location & Seq
Reverse (-)
15886464 .. 15887039
576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1161400.v1.1.491

Sequence Viewer

Length: 576 bp
ATGGATTCAAAGAAGTCTAACAAGATTAGGGAGATTGTTAGGCTCCAACAGATCCTAAAGAAGTGGAAAAAGCTAGCAAATGCTTCTAAGAACACCACCACAACCAATTCCTCATCAGCTAATACCATAGTGATGAATAATGTCAATAGTACTACTACTAGTACTAGCAGCAATGGCAGCAGCAGAAGCATGAAGTTCTTCAAGAGAACACTCTCCTTCACAGACGTTTCCGCGGCTCAAAATGACATCGTGCCAAAAGGGTTTCTTGCCATGTGTGTTGGGAAGGAGCTGAAGAGATTTATCATCCCAACTGAGTACTTGGGTCACCAAGCTTTTCGGATTCTGCTCCAGGAAGCCGAAGAGGAATTCGGGTTTCAGCAAGCAGGTGTGCTCAAGATTCCATGTGAAGTGTCAGTGTTTGAGAAGATCTTGAAAATGGTTGAAGAGAAAAGGGAGGTGTTTTCCTTGCACAATGAGTTTGGATTCATTAATGCAGAGAAAGACATGATTGGCTTTTGCTCATCTCCATCAGATTGTGAGCTTACACCATCTCATCACCCTCAAATGTGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

192

Amino Acids

21.54

Weight (kDa)

9.11

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 39 - 147 4.5e-25 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 374
Acc36I ACCTGC 1 cut(s) 374
AccII CGCG 1 cut(s) 233
AciI CCGC 2 cut(s) 231, 233
AclWI GGATC 1 cut(s) 46
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 1 cut(s) 311
AfaI GTAC 3 cut(s) 151, 163, 317
AgsI TTSAA 4 cut(s) 9, 202, 433, 443
AhlI ACTAGT 1 cut(s) 158
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 5 cut(s) 73, 119, 289, 332, 541
AluI AGCT 5 cut(s) 73, 119, 289, 332, 541
Alw21I GWGCWC 1 cut(s) 393
AlwI GGATC 1 cut(s) 46
ApeKI GCWGC 3 cut(s) 168, 177, 180
ApoI RAATTY 1 cut(s) 365
AseI ATTAAT 1 cut(s) 489
Asp700I GAANNNNTTC 1 cut(s) 197
AsuHPI GGTGA 2 cut(s) 317, 548
AsuNHI GCTAGC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 3 cut(s) 180, 189, 192
BccI CCATC 2 cut(s) 535, 556
BciT130I CCWGG 1 cut(s) 350
BcuI ACTAGT 1 cut(s) 158
BfaI CTAG 3 cut(s) 74, 159, 165
BfuAI ACCTGC 1 cut(s) 374
BglII AGATCT 1 cut(s) 426
BisI GCNGC 4 cut(s) 169, 178, 181, 234
BlsI GCNGC 4 cut(s) 170, 179, 182, 235
BmcAI AGTACT 3 cut(s) 151, 163, 317
Bme1390I CCNGG 1 cut(s) 350
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 1 cut(s) 350
BmtI GCTAGC 1 cut(s) 77
BpmI CTGGAG 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 231
Bse3DI GCAATG 1 cut(s) 178
BseBI CCWGG 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 1 cut(s) 303
BseMI GCAATG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 303
BseXI GCAGC 3 cut(s) 180, 189, 192
Bsh1236I CGCG 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 393
Bsp143I GATC 2 cut(s) 51, 426
BspACI CCGC 2 cut(s) 231, 233
BspCNI CTCAG 1 cut(s) 304
BspFNI CGCG 1 cut(s) 233
BspLI GGNNCC 1 cut(s) 44
BspMI ACCTGC 1 cut(s) 374
BspOI GCTAGC 1 cut(s) 77
BspPI GGATC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 178
BssECI CCNNGG 1 cut(s) 231
BssMI GATC 2 cut(s) 51, 426
Bst2UI CCWGG 1 cut(s) 350
Bst6I CTCTTC 3 cut(s) 287, 354, 438
BstC8I GCNNGC 2 cut(s) 75, 381
BstDEI CTNAG 2 cut(s) 87, 312
BstDSI CCRYGG 1 cut(s) 231
BstEII GGTNACC 1 cut(s) 323
BstF5I GGATG 1 cut(s) 303
BstFNI CGCG 1 cut(s) 233
BstKTI GATC 2 cut(s) 54, 429
BstMBI GATC 2 cut(s) 51, 426
BstMWI GCNNNNNNNGC 3 cut(s) 174, 177, 186
BstNI CCWGG 1 cut(s) 350
BstPI GGTNACC 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 348
BstUI CGCG 1 cut(s) 233
BstV1I GCAGC 3 cut(s) 180, 189, 192
BstX2I RGATCY 2 cut(s) 51, 426
BstYI RGATCY 2 cut(s) 51, 426
BtgI CCRYGG 1 cut(s) 231
BtsCI GGATG 1 cut(s) 303
BtsIMutI CAGTG 1 cut(s) 420
BveI ACCTGC 1 cut(s) 374
Cac8I GCNNGC 2 cut(s) 75, 381
Cfr42I CCGCGG 1 cut(s) 234
Csp6I GTAC 3 cut(s) 150, 162, 316
CviAII CATG 4 cut(s) 190, 271, 402, 505
CviJI RGCY 9 cut(s) 43, 73, 119, 236, 289, 332, 356, 513, 541
CviKI_1 RGCY 9 cut(s) 43, 73, 119, 236, 289, 332, 356, 513, 541
CviQI GTAC 3 cut(s) 150, 162, 316
DdeI CTNAG 2 cut(s) 87, 312
DpnI GATC 2 cut(s) 53, 428
DpnII GATC 2 cut(s) 51, 426
Eam1104I CTCTTC 3 cut(s) 287, 354, 438
EarI CTCTTC 3 cut(s) 287, 354, 438
Eco57I CTGAAG 1 cut(s) 311
Eco91I GGTNACC 1 cut(s) 323
EcoO65I GGTNACC 1 cut(s) 323
EcoRI GAATTC 1 cut(s) 365
EcoRII CCWGG 1 cut(s) 348
FaeI CATG 4 cut(s) 193, 274, 405, 508
FaiI YATR 5 cut(s) 128, 191, 272, 403, 506
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FatI CATG 4 cut(s) 189, 270, 401, 504
Fnu4HI GCNGC 4 cut(s) 169, 178, 181, 234
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 4 cut(s) 169, 178, 181, 234
FspBI CTAG 3 cut(s) 74, 159, 165
GluI GCNGC 4 cut(s) 169, 178, 181, 234
GsuI CTGGAG 1 cut(s) 332
Hin1II CATG 4 cut(s) 193, 274, 405, 508
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 4 cut(s) 5, 340, 397, 483
HphI GGTGA 2 cut(s) 317, 548
Hpy188I TCNGA 2 cut(s) 339, 532
Hpy188III TCNNGA 3 cut(s) 202, 394, 430
HpyAV CCTTC 2 cut(s) 226, 277
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 469, 494, 570
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 177, 186
HpyF3I CTNAG 2 cut(s) 87, 312
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 4 cut(s) 193, 274, 405, 508
KspI CCGCGG 1 cut(s) 234
Kzo9I GATC 2 cut(s) 51, 426
LmnI GCTCC 3 cut(s) 48, 286, 351
LpnPI CCDG 3 cut(s) 335, 362, 369
Lsp1109I GCAGC 3 cut(s) 180, 189, 192
MaeI CTAG 3 cut(s) 74, 159, 165
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 323
MalI GATC 2 cut(s) 53, 428
MboI GATC 2 cut(s) 51, 426
MboII GAAGA 5 cut(s) 190, 304, 371, 436, 455
MflI RGATCY 2 cut(s) 51, 426
MhlI GDGCHC 1 cut(s) 393
MluCI AATT 2 cut(s) 106, 365
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 4 cut(s) 121, 355, 448, 570
MroXI GAANNNNTTC 1 cut(s) 197
MseI TTAA 1 cut(s) 489
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 233
MspR9I CCNGG 1 cut(s) 350
MvaI CCWGG 1 cut(s) 350
MvnI CGCG 1 cut(s) 233
MwoI GCNNNNNNNGC 3 cut(s) 174, 177, 186
NdeII GATC 2 cut(s) 51, 426
NheI GCTAGC 1 cut(s) 73
NlaIII CATG 4 cut(s) 193, 274, 405, 508
NlaIV GGNNCC 1 cut(s) 44
NmuCI GTSAC 1 cut(s) 323
PaqCI CACCTGC 1 cut(s) 374
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 4 cut(s) 5, 340, 397, 483
PfoI TCCNGGA 1 cut(s) 348
PkrI GCNGC 4 cut(s) 170, 179, 182, 235
PshBI ATTAAT 1 cut(s) 489
Psp6I CCWGG 1 cut(s) 348
PspEI GGTNACC 1 cut(s) 323
PspGI CCWGG 1 cut(s) 348
PspN4I GGNNCC 1 cut(s) 44
PsuI RGATCY 2 cut(s) 51, 426
RsaI GTAC 3 cut(s) 151, 163, 317
RsaNI GTAC 3 cut(s) 150, 162, 316
RseI CAYNNNNRTG 1 cut(s) 131
SacII CCGCGG 1 cut(s) 234
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 4 cut(s) 169, 178, 181, 234
Sau3AI GATC 2 cut(s) 51, 426
ScaI AGTACT 3 cut(s) 151, 163, 317
ScrFI CCNGG 1 cut(s) 350
SduI GDGCHC 1 cut(s) 393
SetI ASST 8 cut(s) 75, 121, 228, 291, 334, 388, 459, 543
Sfr303I CCGCGG 1 cut(s) 234
SgrBI CCGCGG 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
SpeI ACTAGT 1 cut(s) 158
Sse9I AATT 2 cut(s) 106, 365
SsiI CCGC 2 cut(s) 231, 233
SspMI CTAG 3 cut(s) 74, 159, 165
StyD4I CCNGG 1 cut(s) 348
TaiI ACGT 1 cut(s) 228
TasI AATT 2 cut(s) 106, 365
TatI WGTACW 3 cut(s) 149, 161, 315
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 4 cut(s) 5, 340, 397, 483
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 1 cut(s) 420
TseFI GTSAC 1 cut(s) 323
TseI GCWGC 3 cut(s) 168, 177, 180
Tsp45I GTSAC 1 cut(s) 323
TspDTI ATGAA 3 cut(s) 149, 206, 475
TspRI CASTG 1 cut(s) 420
VspI ATTAAT 1 cut(s) 489
XapI RAATTY 1 cut(s) 365
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 3 cut(s) 74, 159, 165
ZrmI AGTACT 3 cut(s) 151, 163, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.