MD09G1166200.v1.1

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006199
Physical Location & Seq
Forward (+)
13620973 .. 13621542
570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1166200.v1.1.491

Sequence Viewer

Length: 570 bp
ATGGATTCAAAGAAGTCTAACAAGATTAGGGAGATTGTTAGGCTCCAACAGATCTTAAAGAAGTGGAAAAAGCTAGCAAACGCTCCTAAAAACACCACGACAACCAATTCCTCATCAGCTTATAGCATAATTAATAATGCCAATAGCACTACCACTAGTACCAGCAGCAATGGCAGCAGCAGAAGCATGAAGTTCTTCAAGAGAACACTCTCTTTCTCAGATGTTTCCAAGACTCAAAATGTCATCGTGCCAAAAGGGTTTCTTGCAATGTGTGTTGGGAAGGAGCTGAAGAGATTCGTCGTCCCAACTGAGTACTTGGGTCACCAAGCTTTTAGGATTCTGCTCCAGGAAGCCGAAGAGGAATTCGGGTTTCAGCGAGAAGGTGTGCTCAAGATTCCATGTGAAGTGTCAGCGTTTGAGAAGATCTTGAAGATTGTTGAAGAGAAAAGGGAGGTCTTTTCCTTGCACAATGAGTTTGGATTCATTAATGCAGAGAAAGACATGATTGGTTTTTGCTCGTCTCCATCAGATTGTGAGATTACACCTCATCACCCTCAAATGTGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

190

Amino Acids

21.42

Weight (kDa)

9.32

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 40 - 145 4.3e-24 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 362
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 2 cut(s) 160, 314
AgsI TTSAA 4 cut(s) 9, 199, 430, 440
AhlI ACTAGT 1 cut(s) 155
AjnI CCWGG 1 cut(s) 345
AluBI AGCT 4 cut(s) 73, 119, 286, 329
AluI AGCT 4 cut(s) 73, 119, 286, 329
Alw21I GWGCWC 1 cut(s) 390
Alw26I GTCTC 1 cut(s) 525
ApeKI GCWGC 3 cut(s) 165, 174, 177
ApoI RAATTY 1 cut(s) 362
AseI ATTAAT 2 cut(s) 132, 486
Asp700I GAANNNNTTC 2 cut(s) 194, 293
AsuHPI GGTGA 2 cut(s) 314, 542
AsuNHI GCTAGC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 3 cut(s) 177, 186, 189
BccI CCATC 1 cut(s) 532
BciT130I CCWGG 1 cut(s) 347
BcoDI GTCTC 1 cut(s) 525
BcuI ACTAGT 1 cut(s) 155
BfaI CTAG 2 cut(s) 74, 156
BglII AGATCT 2 cut(s) 51, 423
BisI GCNGC 3 cut(s) 166, 175, 178
BlsI GCNGC 3 cut(s) 167, 176, 179
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 1 cut(s) 347
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 1 cut(s) 347
BmtI GCTAGC 1 cut(s) 77
BpmI CTGGAG 1 cut(s) 329
BpuEI CTTGAG 1 cut(s) 374
Bse3DI GCAATG 2 cut(s) 175, 273
BseBI CCWGG 1 cut(s) 347
BseMI GCAATG 2 cut(s) 175, 273
BseMII CTCAG 2 cut(s) 231, 300
BseXI GCAGC 3 cut(s) 177, 186, 189
BsiHKAI GWGCWC 1 cut(s) 390
BslFI GGGAC 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 525
BsmBI CGTCTC 1 cut(s) 525
BsmFI GGGAC 1 cut(s) 287
Bsp1286I GDGCHC 1 cut(s) 390
Bsp143I GATC 2 cut(s) 51, 423
BspCNI CTCAG 2 cut(s) 230, 301
BspLI GGNNCC 1 cut(s) 44
BspOI GCTAGC 1 cut(s) 77
BsrDI GCAATG 2 cut(s) 175, 273
BssMI GATC 2 cut(s) 51, 423
Bst2UI CCWGG 1 cut(s) 347
Bst6I CTCTTC 3 cut(s) 284, 351, 435
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 2 cut(s) 217, 309
BstEII GGTNACC 1 cut(s) 320
BstKTI GATC 2 cut(s) 54, 426
BstMAI GTCTC 1 cut(s) 525
BstMBI GATC 2 cut(s) 51, 423
BstMWI GCNNNNNNNGC 3 cut(s) 171, 174, 183
BstNI CCWGG 1 cut(s) 347
BstPI GGTNACC 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 345
BstV1I GCAGC 3 cut(s) 177, 186, 189
BstX2I RGATCY 2 cut(s) 51, 423
BstYI RGATCY 2 cut(s) 51, 423
Cac8I GCNNGC 1 cut(s) 75
Csp6I GTAC 2 cut(s) 159, 313
CviAII CATG 3 cut(s) 187, 399, 502
CviJI RGCY 6 cut(s) 43, 73, 119, 286, 329, 353
CviKI_1 RGCY 6 cut(s) 43, 73, 119, 286, 329, 353
CviQI GTAC 2 cut(s) 159, 313
DdeI CTNAG 2 cut(s) 217, 309
DpnI GATC 2 cut(s) 53, 425
DpnII GATC 2 cut(s) 51, 423
Eam1104I CTCTTC 3 cut(s) 284, 351, 435
EarI CTCTTC 3 cut(s) 284, 351, 435
Eco57I CTGAAG 1 cut(s) 308
Eco91I GGTNACC 1 cut(s) 320
EcoO65I GGTNACC 1 cut(s) 320
EcoRI GAATTC 1 cut(s) 362
EcoRII CCWGG 1 cut(s) 345
Esp3I CGTCTC 1 cut(s) 525
FaeI CATG 3 cut(s) 190, 402, 505
FaiI YATR 5 cut(s) 123, 128, 188, 400, 503
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FaqI GGGAC 1 cut(s) 287
FatI CATG 3 cut(s) 186, 398, 501
Fnu4HI GCNGC 3 cut(s) 166, 175, 178
Fsp4HI GCNGC 3 cut(s) 166, 175, 178
FspBI CTAG 2 cut(s) 74, 156
GluI GCNGC 3 cut(s) 166, 175, 178
GsuI CTGGAG 1 cut(s) 329
Hin1II CATG 3 cut(s) 190, 402, 505
HindIII AAGCTT 1 cut(s) 327
HinfI GANTC 6 cut(s) 5, 232, 294, 337, 394, 480
HphI GGTGA 2 cut(s) 314, 542
Hpy188I TCNGA 2 cut(s) 220, 529
Hpy188III TCNNGA 3 cut(s) 199, 391, 427
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 2 cut(s) 274, 374
HpyCH4V TGCA 4 cut(s) 266, 466, 491, 564
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 174, 183
HpyF3I CTNAG 2 cut(s) 217, 309
Hsp92II CATG 3 cut(s) 190, 402, 505
Kzo9I GATC 2 cut(s) 51, 423
LmnI GCTCC 4 cut(s) 48, 88, 283, 348
LpnPI CCDG 3 cut(s) 175, 332, 359
Lsp1109I GCAGC 3 cut(s) 177, 186, 189
MaeI CTAG 2 cut(s) 74, 156
MaeIII GTNAC 1 cut(s) 320
MalI GATC 2 cut(s) 53, 425
MboI GATC 2 cut(s) 51, 423
MboII GAAGA 6 cut(s) 187, 301, 368, 433, 442, 452
MflI RGATCY 2 cut(s) 51, 423
MhlI GDGCHC 1 cut(s) 390
MluCI AATT 3 cut(s) 106, 129, 362
MlyI GAGTC 1 cut(s) 226
MmeI TCCRAC 1 cut(s) 70
MnlI CCTC 5 cut(s) 121, 352, 445, 555, 564
MroXI GAANNNNTTC 2 cut(s) 194, 293
MseI TTAA 3 cut(s) 56, 132, 486
MspR9I CCNGG 1 cut(s) 347
MvaI CCWGG 1 cut(s) 347
MwoI GCNNNNNNNGC 3 cut(s) 171, 174, 183
NdeII GATC 2 cut(s) 51, 423
NheI GCTAGC 1 cut(s) 73
NlaIII CATG 3 cut(s) 190, 402, 505
NlaIV GGNNCC 1 cut(s) 44
NmuCI GTSAC 1 cut(s) 320
PdmI GAANNNNTTC 2 cut(s) 194, 293
PfeI GAWTC 5 cut(s) 5, 294, 337, 394, 480
PfoI TCCNGGA 1 cut(s) 345
PkrI GCNGC 3 cut(s) 167, 176, 179
PleI GAGTC 1 cut(s) 226
PpsI GAGTC 1 cut(s) 226
PshBI ATTAAT 2 cut(s) 132, 486
Psp6I CCWGG 1 cut(s) 345
PspEI GGTNACC 1 cut(s) 320
PspGI CCWGG 1 cut(s) 345
PspN4I GGNNCC 1 cut(s) 44
PsuI RGATCY 2 cut(s) 51, 423
RsaI GTAC 2 cut(s) 160, 314
RsaNI GTAC 2 cut(s) 159, 313
SaqAI TTAA 3 cut(s) 56, 132, 486
SatI GCNGC 3 cut(s) 166, 175, 178
Sau3AI GATC 2 cut(s) 51, 423
ScaI AGTACT 1 cut(s) 314
SchI GAGTC 1 cut(s) 226
ScrFI CCNGG 1 cut(s) 347
SduI GDGCHC 1 cut(s) 390
SetI ASST 7 cut(s) 75, 121, 288, 331, 385, 456, 547
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
SpeI ACTAGT 1 cut(s) 155
Sse9I AATT 3 cut(s) 106, 129, 362
SspMI CTAG 2 cut(s) 74, 156
StyD4I CCNGG 1 cut(s) 345
TasI AATT 3 cut(s) 106, 129, 362
TatI WGTACW 1 cut(s) 312
TfiI GAWTC 5 cut(s) 5, 294, 337, 394, 480
Tru1I TTAA 3 cut(s) 56, 132, 486
Tru9I TTAA 3 cut(s) 56, 132, 486
TseFI GTSAC 1 cut(s) 320
TseI GCWGC 3 cut(s) 165, 174, 177
Tsp45I GTSAC 1 cut(s) 320
TspDTI ATGAA 2 cut(s) 203, 472
VspI ATTAAT 2 cut(s) 132, 486
XapI RAATTY 1 cut(s) 362
XmnI GAANNNNTTC 2 cut(s) 194, 293
XspI CTAG 2 cut(s) 74, 156
ZrmI AGTACT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.