MD15G1102700.v1.1

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006199
Physical Location & Seq
Forward (+)
7267646 .. 7268164
519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1102700.v1.1.491

Sequence Viewer

Length: 519 bp
ATGAACAACACCATGGATTCAAAGACATCAAACAAGATCAGAGACATTGTCAGGCTCCAACAGATTCTCAAAAAGTGGAGAAAGATTGCTAACTCATCAAAAGCAATATCCGCCGCCACCACTACCACCTCCACCAGCAAGAGCATCAAGTTTCTGAAGAGGACACTTTCTCTGTCTGAAAAAACAAACACTTCATGTGAAGCCTCAAACAATGCTGCTGTCCCAAAAGGCTACCTTGCTGTCTGTGTTGGGGAAGAGCTCAAGAGGTTTGTCATCCCAACCGACTACTTGGGTGGGCCAGCTTTCCATATTCTGTTGAGAGAGGCGGAGGAGGAGTTTGGGTTTCAGCAGACCGGCGTTTTGAGGATTCCATGTGAGGTTTCTGTGTTTGAAGGTATTCTGAAGATGGTGGAGGAAGACAAGGACTTGTTCTTCAATAATATGGTGGGAGATTGCTCAACAGCATCAGATGGCCAGCTCAACTTTTCTCACCACCCAGAAAACCCAATGTGCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

173

Amino Acids

19.11

Weight (kDa)

7.62

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 18 - 137 2.3e-27 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 111, 114, 326
AcoI YGGCCR 1 cut(s) 472
AcuI CTGAAG 2 cut(s) 176, 422
AgsI TTSAA 3 cut(s) 21, 392, 436
AluBI AGCT 3 cut(s) 259, 302, 478
AluI AGCT 3 cut(s) 259, 302, 478
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 36
AoxI GGCC 2 cut(s) 296, 472
ApeKI GCWGC 1 cut(s) 215
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
Asp700I GAANNNNTTC 1 cut(s) 396
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 482
BalI TGGCCA 1 cut(s) 474
BanII GRGCYC 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 202
BccI CCATC 2 cut(s) 400, 464
BcoDI GTCTC 1 cut(s) 36
BisI GCNGC 2 cut(s) 114, 216
BlsI GCNGC 2 cut(s) 115, 217
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 1 cut(s) 56
BmsI GCATC 2 cut(s) 153, 473
BpiI GAAGAC 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 12
BsaXI ACNNNNNCTCC 4 cut(s) 320, 326, 350, 356
Bse118I RCCGGY 1 cut(s) 353
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 1 cut(s) 273
BseRI GAGGAG 2 cut(s) 344, 347
BseXI GCAGC 1 cut(s) 202
BshFI GGCC 2 cut(s) 298, 474
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 1 cut(s) 354
BslFI GGGAC 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 36
BsmFI GGGAC 1 cut(s) 206
BsnI GGCC 2 cut(s) 298, 474
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 1 cut(s) 36
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 3 cut(s) 111, 114, 326
BspANI GGCC 2 cut(s) 298, 474
BspLI GGNNCC 1 cut(s) 56
BspQI GCTCTTC 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 353
BssAI RCCGGY 1 cut(s) 353
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 12
Bst6I CTCTTC 2 cut(s) 152, 249
BstC8I GCNNGC 2 cut(s) 300, 476
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 1 cut(s) 273
BstKTI GATC 1 cut(s) 39
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 1 cut(s) 36
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstV1I GCAGC 1 cut(s) 202
BstV2I GAAGAC 1 cut(s) 423
BsuRI GGCC 2 cut(s) 298, 474
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 1 cut(s) 273
Cac8I GCNNGC 2 cut(s) 300, 476
Cfr10I RCCGGY 1 cut(s) 353
Cfr13I GGNCC 1 cut(s) 296
CviAII CATG 3 cut(s) 13, 195, 372
CviJI RGCY 8 cut(s) 55, 203, 231, 259, 298, 302, 474, 478
CviKI_1 RGCY 8 cut(s) 55, 203, 231, 259, 298, 302, 474, 478
DpnI GATC 1 cut(s) 38
DpnII GATC 1 cut(s) 36
EaeI YGGCCR 1 cut(s) 472
Eam1104I CTCTTC 2 cut(s) 152, 249
EarI CTCTTC 2 cut(s) 152, 249
EciI GGCGGA 2 cut(s) 100, 341
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 261
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 2 cut(s) 176, 422
EcoICRI GAGCTC 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 3 cut(s) 16, 198, 375
FaiI YATR 7 cut(s) 14, 196, 309, 373, 443, 515, 517
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FaqI GGGAC 1 cut(s) 206
FatI CATG 3 cut(s) 12, 194, 371
Fnu4HI GCNGC 2 cut(s) 114, 216
FokI GGATG 1 cut(s) 260
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 2 cut(s) 114, 216
GluI GCNGC 2 cut(s) 114, 216
HaeIII GGCC 2 cut(s) 298, 474
HapII CCGG 1 cut(s) 354
Hin1II CATG 3 cut(s) 16, 198, 375
HinfI GANTC 3 cut(s) 17, 64, 367
HpaII CCGG 1 cut(s) 354
HphI GGTGA 1 cut(s) 482
Hpy188I TCNGA 5 cut(s) 41, 156, 178, 402, 469
Hpy188III TCNNGA 1 cut(s) 262
HpyAV CCTTC 1 cut(s) 386
HpyCH4V TGCA 1 cut(s) 513
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
Hsp92II CATG 3 cut(s) 16, 198, 375
Kzo9I GATC 1 cut(s) 36
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 6 cut(s) 37, 148, 312, 367, 488, 510
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 2 cut(s) 153, 473
MalI GATC 1 cut(s) 38
MboI GATC 1 cut(s) 36
MboII GAAGA 5 cut(s) 169, 266, 415, 424, 428
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 1 cut(s) 474
MluNI TGGCCA 1 cut(s) 474
MmeI TCCRAC 1 cut(s) 82
Mox20I TGGCCA 1 cut(s) 474
MroXI GAANNNNTTC 1 cut(s) 396
MscI TGGCCA 1 cut(s) 474
Msp20I TGGCCA 1 cut(s) 474
MspI CCGG 1 cut(s) 354
MwoI GCNNNNNNNGC 1 cut(s) 110
NcoI CCATGG 1 cut(s) 12
NdeII GATC 1 cut(s) 36
NlaIII CATG 3 cut(s) 16, 198, 375
NlaIV GGNNCC 1 cut(s) 56
PciSI GCTCTTC 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 3 cut(s) 17, 64, 367
PflFI GACNNNGTC 1 cut(s) 47
PkrI GCNGC 2 cut(s) 115, 217
Psp124BI GAGCTC 1 cut(s) 261
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 296
PsyI GACNNNGTC 1 cut(s) 47
SacI GAGCTC 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 249
SatI GCNGC 2 cut(s) 114, 216
Sau3AI GATC 1 cut(s) 36
Sau96I GGNCC 1 cut(s) 296
SduI GDGCHC 1 cut(s) 261
SetI ASST 8 cut(s) 131, 237, 261, 269, 304, 381, 397, 480
SfaNI GCATC 2 cut(s) 153, 473
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
SsiI CCGC 3 cut(s) 111, 114, 326
SstI GAGCTC 1 cut(s) 261
StyI CCWWGG 1 cut(s) 12
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 3 cut(s) 17, 64, 367
TseI GCWGC 1 cut(s) 215
TspDTI ATGAA 2 cut(s) 17, 183
Tth111I GACNNNGTC 1 cut(s) 47
XmnI GAANNNNTTC 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.