MD08G1131400.v1.1

aarF domain-containing protein kinase At4g31390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001871
Physical Location & Seq
Reverse (-)
12400254 .. 12406419
6166 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1131400.v1.1.491

Sequence Viewer

Length: 2058 bp
ATGAATTTGCTCTGCGTAAACTGCGTAACCGCAGCTCCGTTTCCCGCCAATCAGCACCTGAGCTCGCGAGCTCACACTGTTCCGAGACGCATCAATGCGCCGTCGCGTGCTCCGGCGTCGTTCGATTACAGGCTTCGGATACGAAGAGGCAGGAAACCCTTGCGATTCCGAGTTTCGAATGCCGCAATCGAAACGCGGAGGAGCTTGGCGGCTTCTACTGGCGAAAGGCAATTGAGCAAACCTAGCGCGGCCATGGAGCAGCTCGACTTCGAACGCGGTGTTTGCGTGCCGTTTCGGAAGTACAGTCCGGAAACTGTGAGGTATACGGTGTTGGAATCCAGAGGAGCAATTGTGTCATTACTTTCGAGGGGTGTGGAAATTGTGTGGAGTTTGGGATTTTACTGGTCTACCTTGGCGTATGACTACGTCATAGGAAGGGATGAAGAAGTTGTTTCAGCTCGTGCTCGGCAGCTTAGAAACCTCCTATGTGATTTGGGGCCTTCTTTCATTAAAGCCGGCCAGGTTCTTGCAAACAGACCTGATATTATCAGAGAAGATTATATGAATGAACTCTGCATTCTTCAAGATGACGTTCCTCCCTTCCCCAATCAGGTTGCCTTCGACATAATAGAAGAAGAAATGGGGAGACCCCTAGAATCTGTTTTCAGTAAAATTTCGTCTCAAACGATAGCAGCTGCCAGTTTAGGTCAAGTATACCGAGCTACATTACGAGATTCTGGCGAGGAAGTTGCTATTAAGGTTCAAAGGCCGCAGATAGAACCTATAATTTATCGGGATCTTTTTCTGTTCCGAACTCTTGCTTCATTCTTAAATGGCATCAGTCTACAGAAACTGGGGTGCAATGCTGAGCTGATTGTTGATGAATTTGGTGAGAAGCTTTTGGAGGAGCTTGATTACACTTTGGAAGCCCGCAATATTGAAGATTTTCTTCTGAACTTTGAAGGTGATCCTACTGTCAAAATTCCTCGGGTTTACAAAGAGCTTTGTAGTTCACGTGTTCTAGTGATGGAGTGGATTGATGGTATTCGGTGCACTAACCCACAGACTATTAAAGAAGCTGGTATTGATGTAAATGGGTTCTTAACGGTTGGAGTCAGTGCTGCATTGCGGCAATTGCTAGAATTTGGGTTGTTCCATGGAGATCCACATCCTGGAAATGTTTTTGCCATGCGTGATGGGCGTATTGCATATGTGGACTTTGGGAATGTTGCTGTGCTTAGTCAGCAAAATAAACAAATTTTAATTGATGCTGTTGTCCATGCTGTAAACGAGGACTATGTTGAGATGGCAAATGATTTCACCAGACTTGGCTTCTTGGCTCCTGGGACCGATGTCTCTCCTATTGTTCCAGCTTTAGAATCAATATGGCAGAACTCTGTTGGAAAAGGACTGTCTGACTTTAATTTTCGAAGCGTTACTGGGAAGTTTAACCAATTAGTCTACAACTATCCCATCCGTATCCCAGAGAGGTTTTCCCTTGTCATCCGTTCTTTGTTGACTCAAGAGGGCATCTGTTTTACTTTGCAGCCAGACTTCAAATTTCTTGAGGCAAGGGCAGATCTTGTTATCGTTGCCTATCCATATGTGGCAAAGCGCCTACTGACAGATCCAAACCCAGCTCTACGTGAACGCCTCATACAGGTATTATTTAAAGATGGTGTTTTCCAATGGAAGCGGCTAGAAAACCTTATTGTTCTTGCCAAAGAAAATGTGACCAAGATGAGCAGCAATCCTGCATTTCAAGCTGTAGACACGCAAGGGTCAAAAAAATTGCAAGTTAGTAGGAAACTGGACCTCACTGACACCATCAAAGATGGAGCTCGCCTTTTCATTTTTGATGAAGGAATTCGTAGAAAGCTTATTCTTGCTTTGACGGAAGATTCAAAGCTTCACATTGAAGAGCTTGTGGATGTGTATAGACTCGTTGAAGATCAGATAGACATACCCTCAGTTGCAGTGGAAGTAGCACGAGATTTCCCAACCGTTGTACGAGATCTGATGCTTTCGTGGAGCGAGTCGGTGTTATCTGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006464 GO:0006468 GO:0006725 GO:0006778 GO:0006787 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0006995 GO:0006996 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009267 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009637 GO:0009639 GO:0009657 GO:0009658 GO:0009668 GO:0009767 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0009987 GO:0009991 GO:0010027 GO:0010033 GO:0010035 GO:0010109 GO:0010114 GO:0010287 GO:0010380 GO:0015979 GO:0015994 GO:0015996 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019216 GO:0019222 GO:0019439 GO:0019538 GO:0019684 GO:0019747 GO:0022900 GO:0031279 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031537 GO:0031540 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033015 GO:0033554 GO:0034641 GO:0036211 GO:0042221 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043562 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044270 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046149 GO:0046483 GO:0046700 GO:0046889 GO:0046890 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051186 GO:0051187 GO:0051193 GO:0051194 GO:0051716 GO:0055114 GO:0061024 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080177 GO:0080183 GO:0090056 GO:0140096 GO:1901360 GO:1901361 GO:1901401 GO:1901403 GO:1901463 GO:1901465 GO:1901562 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902171 GO:1902326 GO:1904143
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

686

Amino Acids

77.32

Weight (kDa)

5.83

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 194 - 438 4.2e-72 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1172
AccI GTMKAC 6 cut(s) 323, 407, 714, 844, 1461, 1770
AccII CGCG 5 cut(s) 67, 106, 196, 248, 276
AccIII TCCGGA 1 cut(s) 307
AclWI GGATC 4 cut(s) 804, 962, 1157, 1622
AcoI YGGCCR 2 cut(s) 249, 517
AcsI RAATTY 8 cut(s) 4, 672, 884, 981, 1142, 1257, 1559, 1866
AcvI CACGTG 1 cut(s) 1016
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 2 cut(s) 302, 2010
AfiI CCNNNNNNNGG 3 cut(s) 610, 1172, 1660
AflIII ACRYGT 1 cut(s) 1015
AgsI TTSAA 9 cut(s) 584, 764, 941, 962, 1558, 1763, 1905, 1919, 1949
AjnI CCWGG 3 cut(s) 519, 1171, 1342
Alw21I GWGCWC 6 cut(s) 65, 73, 112, 466, 1055, 1843
Alw26I GTCTC 4 cut(s) 79, 640, 684, 1360
Alw44I GTGCAC 1 cut(s) 1051
AlwI GGATC 4 cut(s) 804, 962, 1157, 1622
AlwNI CAGNNNCTG 2 cut(s) 58, 853
Ama87I CYCGRG 1 cut(s) 987
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 4 cut(s) 249, 497, 517, 767
ApaLI GTGCAC 1 cut(s) 1051
ApeKI GCWGC 8 cut(s) 32, 259, 469, 692, 695, 1121, 1546, 1746
ApoI RAATTY 8 cut(s) 4, 672, 884, 981, 1142, 1257, 1559, 1866
Asp700I GAANNNNTTC 2 cut(s) 945, 1866
AspLEI GCGC 3 cut(s) 100, 248, 1617
AspS9I GGNCC 3 cut(s) 497, 1347, 1813
AsuHPI GGTGA 3 cut(s) 902, 977, 1312
AsuII TTCGAA 3 cut(s) 176, 270, 1429
AvaI CYCGRG 1 cut(s) 987
AvaII GGWCC 2 cut(s) 1347, 1813
BaeGI GKGCMC 1 cut(s) 1055
BanII GRGCYC 3 cut(s) 65, 73, 1843
BauI CACGAG 2 cut(s) 459, 1989
BbrPI CACGTG 1 cut(s) 1016
Bbv12I GWGCWC 6 cut(s) 65, 73, 112, 466, 1055, 1843
BbvI GCAGC 8 cut(s) 44, 271, 481, 682, 704, 1108, 1558, 1758
BccI CCATC 8 cut(s) 1021, 1034, 1190, 1300, 1481, 1670, 1829, 1835
BceAI ACGGC 2 cut(s) 85, 274
BcgI CGANNNNNNTGC 2 cut(s) 731, 765
BciT130I CCWGG 3 cut(s) 521, 1173, 1344
BciVI GTATCC 2 cut(s) 132, 1490
BcoDI GTCTC 4 cut(s) 79, 640, 684, 1360
BfaI CTAG 5 cut(s) 243, 653, 1022, 1139, 1700
BfmI CTRYAG 2 cut(s) 845, 1767
BfoI RGCGCY 1 cut(s) 1618
BfuI GTATCC 2 cut(s) 132, 1490
BglII AGATCT 2 cut(s) 1579, 2014
BlpI GCTNAGC 1 cut(s) 867
Bme1390I CCNGG 3 cut(s) 521, 1173, 1344
Bme18I GGWCC 2 cut(s) 1347, 1813
BmeT110I CYCGRG 1 cut(s) 987
BmgT120I GGNCC 3 cut(s) 497, 1347, 1813
BmiI GGNNCC 3 cut(s) 498, 1341, 1348
BmrFI CCNGG 3 cut(s) 521, 1173, 1344
BmrI ACTGGG 2 cut(s) 863, 1449
BmsI GCATC 5 cut(s) 99, 846, 1258, 1539, 2010
BmuI ACTGGG 2 cut(s) 863, 1449
BoxI GACNNNNGTC 1 cut(s) 1352
Bpu10I CCTNAGC 1 cut(s) 59
Bpu1102I GCTNAGC 1 cut(s) 867
Bpu14I TTCGAA 3 cut(s) 176, 270, 1429
BpuEI CTTGAG 2 cut(s) 1506, 1586
BsaAI YACGTR 2 cut(s) 1016, 1646
BsaBI GATNNNNATC 1 cut(s) 1167
BsaHI GRCGYC 1 cut(s) 116
BsaI GGTCTC 1 cut(s) 640
BsaJI CCNNGG 5 cut(s) 252, 411, 986, 1156, 1343
BsaWI WCCGGW 1 cut(s) 307
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 336, 366
Bsc4I CCNNNNNNNGG 3 cut(s) 610, 1172, 1660
Bse118I RCCGGY 1 cut(s) 515
Bse1I ACTGG 6 cut(s) 223, 407, 699, 858, 1444, 1815
Bse3DI GCAATG 2 cut(s) 868, 1124
Bse8I GATNNNNATC 1 cut(s) 1167
BseAI TCCGGA 1 cut(s) 307
BseBI CCWGG 3 cut(s) 521, 1173, 1344
BseDI CCNNGG 5 cut(s) 252, 411, 986, 1156, 1343
BseGI GGATG 5 cut(s) 445, 1168, 1473, 1503, 1936
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 3 cut(s) 610, 1172, 1660
BseMI GCAATG 2 cut(s) 868, 1124
BseMII CTCAG 3 cut(s) 50, 858, 1983
BseNI ACTGG 6 cut(s) 223, 407, 699, 858, 1444, 1815
BseRI GAGGAG 3 cut(s) 214, 357, 920
BseSI GKGCMC 1 cut(s) 1055
BseXI GCAGC 8 cut(s) 44, 271, 481, 682, 704, 1108, 1558, 1758
BseYI CCCAGC 1 cut(s) 1636
Bsh1236I CGCG 5 cut(s) 67, 106, 196, 248, 276
BshFI GGCC 4 cut(s) 251, 499, 519, 769
BsiHKAI GWGCWC 6 cut(s) 65, 73, 112, 466, 1055, 1843
BsiHKCI CYCGRG 1 cut(s) 987
BsiSI CCGG 3 cut(s) 113, 308, 516
BslFI GGGAC 1 cut(s) 1360
BslI CCNNNNNNNGG 3 cut(s) 610, 1172, 1660
BsmAI GTCTC 4 cut(s) 79, 640, 684, 1360
BsmBI CGTCTC 2 cut(s) 79, 684
BsmFI GGGAC 1 cut(s) 1360
BsmI GAATGC 2 cut(s) 184, 576
BsnI GGCC 4 cut(s) 251, 499, 519, 769
Bso31I GGTCTC 1 cut(s) 640
BsoBI CYCGRG 1 cut(s) 987
Bsp119I TTCGAA 3 cut(s) 176, 270, 1429
Bsp1286I GDGCHC 6 cut(s) 65, 73, 112, 466, 1055, 1843
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 7 cut(s) 796, 967, 1162, 1579, 1627, 1951, 2014
Bsp1720I GCTNAGC 1 cut(s) 867
Bsp19I CCATGG 2 cut(s) 252, 1156
Bsp68I TCGCGA 1 cut(s) 67
BspANI GGCC 4 cut(s) 251, 499, 519, 769
BspCNI CTCAG 3 cut(s) 51, 859, 1982
BspEI TCCGGA 1 cut(s) 307
BspFNI CGCG 5 cut(s) 67, 106, 196, 248, 276
BspLI GGNNCC 3 cut(s) 498, 1341, 1348
BspPI GGATC 4 cut(s) 804, 962, 1157, 1622
BspQI GCTCTTC 1 cut(s) 1914
BspT104I TTCGAA 3 cut(s) 176, 270, 1429
BspTNI GGTCTC 1 cut(s) 640
BsrDI GCAATG 2 cut(s) 868, 1124
BsrFI RCCGGY 1 cut(s) 515
BsrI ACTGG 6 cut(s) 223, 407, 699, 858, 1444, 1815
BssAI RCCGGY 1 cut(s) 515
BssECI CCNNGG 5 cut(s) 252, 411, 986, 1156, 1343
BssMI GATC 7 cut(s) 796, 967, 1162, 1579, 1627, 1951, 2014
BssNAI GTATAC 2 cut(s) 324, 715
BssNI GRCGYC 1 cut(s) 116
BssSI CACGAG 2 cut(s) 459, 1989
BssT1I CCWWGG 3 cut(s) 252, 411, 1156
Bst1107I GTATAC 2 cut(s) 324, 715
Bst2BI CACGAG 2 cut(s) 459, 1989
Bst2UI CCWGG 3 cut(s) 521, 1173, 1344
Bst4CI ACNGT 8 cut(s) 79, 305, 316, 328, 976, 1108, 1413, 2005
Bst6I CTCTTC 2 cut(s) 139, 1914
BstACI GRCGYC 1 cut(s) 116
BstBAI YACGTR 2 cut(s) 1016, 1646
BstBI TTCGAA 3 cut(s) 176, 270, 1429
BstC8I GCNNGC 7 cut(s) 65, 69, 108, 287, 517, 931, 1843
BstDEI CTNAG 5 cut(s) 59, 473, 867, 1238, 1969
BstDSI CCRYGG 2 cut(s) 252, 1156
BstENI CCTNNNNNAGG 1 cut(s) 1658
BstF5I GGATG 5 cut(s) 445, 1168, 1473, 1503, 1936
BstFNI CGCG 5 cut(s) 67, 106, 196, 248, 276
BstH2I RGCGCY 1 cut(s) 1618
BstHHI GCGC 3 cut(s) 100, 248, 1617
BstKTI GATC 7 cut(s) 799, 970, 1165, 1582, 1630, 1954, 2017
BstMAI GTCTC 4 cut(s) 79, 640, 684, 1360
BstMBI GATC 7 cut(s) 796, 967, 1162, 1579, 1627, 1951, 2014
BstMWI GCNNNNNNNGC 7 cut(s) 21, 243, 282, 1135, 1198, 1243, 1763
BstNI CCWGG 3 cut(s) 521, 1173, 1344
BstPAI GACNNNNGTC 1 cut(s) 1352
BstSCI CCNGG 3 cut(s) 519, 1171, 1342
BstSFI CTRYAG 2 cut(s) 845, 1767
BstSLI GKGCMC 1 cut(s) 1055
BstUI CGCG 5 cut(s) 67, 106, 196, 248, 276
BstV1I GCAGC 8 cut(s) 44, 271, 481, 682, 704, 1108, 1558, 1758
BstX2I RGATCY 5 cut(s) 796, 1162, 1579, 1627, 2014
BstYI RGATCY 5 cut(s) 796, 1162, 1579, 1627, 2014
BstZ17I GTATAC 2 cut(s) 324, 715
BsuI GTATCC 2 cut(s) 132, 1490
BsuRI GGCC 4 cut(s) 251, 499, 519, 769
BtgI CCRYGG 2 cut(s) 252, 1156
BtsCI GGATG 5 cut(s) 445, 1168, 1473, 1503, 1936
BtsI GCAGTG 1 cut(s) 1983
BtsIMutI CAGTG 4 cut(s) 75, 1123, 1818, 1983
BtuMI TCGCGA 1 cut(s) 67
Cac8I GCNNGC 7 cut(s) 65, 69, 108, 287, 517, 931, 1843
CaiI CAGNNNCTG 2 cut(s) 58, 853
CfoI GCGC 3 cut(s) 100, 248, 1617
Cfr10I RCCGGY 1 cut(s) 515
Cfr13I GGNCC 3 cut(s) 497, 1347, 1813
CseI GACGC 2 cut(s) 96, 105
Csp6I GTAC 2 cut(s) 301, 2009
CviAII CATG 4 cut(s) 253, 1157, 1189, 1280
CviQI GTAC 2 cut(s) 301, 2009
DdeI CTNAG 5 cut(s) 59, 473, 867, 1238, 1969
DpnI GATC 7 cut(s) 798, 969, 1164, 1581, 1629, 1953, 2016
DpnII GATC 7 cut(s) 796, 967, 1162, 1579, 1627, 1951, 2014
DraI TTTAAA 1 cut(s) 1672
EaeI YGGCCR 2 cut(s) 249, 517
Eam1104I CTCTTC 2 cut(s) 139, 1914
EarI CTCTTC 2 cut(s) 139, 1914
Ecl136II GAGCTC 3 cut(s) 63, 71, 1841
Eco130I CCWWGG 3 cut(s) 252, 411, 1156
Eco24I GRGCYC 3 cut(s) 65, 73, 1843
Eco31I GGTCTC 1 cut(s) 640
Eco47I GGWCC 2 cut(s) 1347, 1813
Eco53kI GAGCTC 3 cut(s) 63, 71, 1841
Eco72I CACGTG 1 cut(s) 1016
Eco88I CYCGRG 1 cut(s) 987
EcoICRI GAGCTC 3 cut(s) 63, 71, 1841
EcoNI CCTNNNNNAGG 1 cut(s) 1658
EcoO109I RGGNCCY 1 cut(s) 497
EcoRI GAATTC 1 cut(s) 1866
EcoRII CCWGG 3 cut(s) 519, 1171, 1342
EcoT14I CCWWGG 3 cut(s) 252, 411, 1156
EcoT38I GRGCYC 3 cut(s) 65, 73, 1843
ErhI CCWWGG 3 cut(s) 252, 411, 1156
Esp3I CGTCTC 2 cut(s) 79, 684
FaeI CATG 4 cut(s) 256, 1160, 1192, 1283
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 1 cut(s) 1360
FatI CATG 4 cut(s) 252, 1156, 1188, 1279
FauI CCCGC 2 cut(s) 52, 938
FauNDI CATATG 2 cut(s) 1210, 1603
FblI GTMKAC 6 cut(s) 323, 407, 714, 844, 1461, 1770
FokI GGATG 5 cut(s) 452, 1155, 1460, 1490, 1943
FriOI GRGCYC 3 cut(s) 65, 73, 1843
FspBI CTAG 5 cut(s) 243, 653, 1022, 1139, 1700
GlaI GCGC 3 cut(s) 99, 247, 1616
GsaI CCCAGC 1 cut(s) 1640
HaeII RGCGCY 1 cut(s) 1618
HaeIII GGCC 4 cut(s) 251, 499, 519, 769
HapII CCGG 3 cut(s) 113, 308, 516
HgaI GACGC 2 cut(s) 96, 105
HhaI GCGC 3 cut(s) 100, 248, 1617
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 4 cut(s) 256, 1160, 1192, 1283
Hin6I GCGC 3 cut(s) 98, 246, 1615
HinP1I GCGC 3 cut(s) 98, 246, 1615
HincII GTYRAC 1 cut(s) 1518
HindII GTYRAC 1 cut(s) 1518
HindIII AAGCTT 3 cut(s) 896, 1877, 1907
HpaII CCGG 3 cut(s) 113, 308, 516
HphI GGTGA 3 cut(s) 902, 977, 1312
Hpy188III TCNNGA 7 cut(s) 66, 308, 339, 584, 794, 1523, 1565
Hpy99I CGWCG 2 cut(s) 106, 121
HpyAV CCTTC 6 cut(s) 429, 510, 610, 628, 956, 1856
HpyCH4III ACNGT 8 cut(s) 79, 305, 316, 328, 976, 1108, 1413, 2005
HpyCH4IV ACGT 4 cut(s) 426, 591, 1015, 1645
HpyF10VI GCNNNNNNNGC 7 cut(s) 21, 243, 282, 1135, 1198, 1243, 1763
HpyF3I CTNAG 5 cut(s) 59, 473, 867, 1238, 1969
HpySE526I ACGT 4 cut(s) 426, 591, 1015, 1645
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 4 cut(s) 256, 1160, 1192, 1283
HspAI GCGC 3 cut(s) 98, 246, 1615
Kpn2I TCCGGA 1 cut(s) 307
KroI GCCGGC 1 cut(s) 515
KroNI GCCGGC 1 cut(s) 517
Kzo9I GATC 7 cut(s) 796, 967, 1162, 1579, 1627, 1951, 2014
LguI GCTCTTC 1 cut(s) 1914
LmnI GCTCC 9 cut(s) 40, 115, 201, 256, 344, 907, 1345, 1838, 2031
Lsp1109I GCAGC 8 cut(s) 44, 271, 481, 682, 704, 1108, 1558, 1758
LweI GCATC 5 cut(s) 99, 846, 1258, 1539, 2010
MaeI CTAG 5 cut(s) 243, 653, 1022, 1139, 1700
MaeII ACGT 4 cut(s) 426, 591, 1015, 1645
MaeIII GTNAC 3 cut(s) 25, 1435, 1732
MalI GATC 7 cut(s) 798, 969, 1164, 1581, 1629, 1953, 2016
MboI GATC 7 cut(s) 796, 967, 1162, 1579, 1627, 1951, 2014
MfeI CAATTG 3 cut(s) 230, 348, 1133
MflI RGATCY 5 cut(s) 796, 1162, 1579, 1627, 2014
MhlI GDGCHC 6 cut(s) 65, 73, 112, 466, 1055, 1843
MlyI GAGTC 4 cut(s) 1122, 1513, 1935, 2045
MmeI TCCRAC 3 cut(s) 312, 1090, 1381
MroI TCCGGA 1 cut(s) 307
MroNI GCCGGC 1 cut(s) 515
MroXI GAANNNNTTC 2 cut(s) 945, 1866
MseI TTAA 9 cut(s) 510, 756, 830, 1071, 1103, 1262, 1422, 1449, 1671
MspA1I CMGCKG 1 cut(s) 695
MspI CCGG 3 cut(s) 113, 308, 516
MspR9I CCNGG 3 cut(s) 521, 1173, 1344
MunI CAATTG 3 cut(s) 230, 348, 1133
Mva1269I GAATGC 2 cut(s) 184, 576
MvaI CCWGG 3 cut(s) 521, 1173, 1344
MvnI CGCG 5 cut(s) 67, 106, 196, 248, 276
MwoI GCNNNNNNNGC 7 cut(s) 21, 243, 282, 1135, 1198, 1243, 1763
NaeI GCCGGC 1 cut(s) 517
NcoI CCATGG 2 cut(s) 252, 1156
NdeI CATATG 2 cut(s) 1210, 1603
NdeII GATC 7 cut(s) 796, 967, 1162, 1579, 1627, 1951, 2014
NgoMIV GCCGGC 1 cut(s) 515
NlaIII CATG 4 cut(s) 256, 1160, 1192, 1283
NlaIV GGNNCC 3 cut(s) 498, 1341, 1348
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 1732
NruI TCGCGA 1 cut(s) 67
NspV TTCGAA 3 cut(s) 176, 270, 1429
PciSI GCTCTTC 1 cut(s) 1914
PcsI WCGNNNNNNNCGW 1 cut(s) 683
PctI GAATGC 2 cut(s) 184, 576
PdiI GCCGGC 1 cut(s) 517
PdmI GAANNNNTTC 2 cut(s) 945, 1866
PfeI GAWTC 6 cut(s) 165, 335, 656, 734, 1379, 1901
PflFI GACNNNGTC 1 cut(s) 425
PflMI CCANNNNNTGG 1 cut(s) 1172
PfoI TCCNGGA 1 cut(s) 1171
PleI GAGTC 4 cut(s) 1121, 1513, 1935, 2044
PmaCI CACGTG 1 cut(s) 1016
PmlI CACGTG 1 cut(s) 1016
PpsI GAGTC 4 cut(s) 1121, 1513, 1935, 2044
Ppu21I YACGTR 2 cut(s) 1016, 1646
PshAI GACNNNNGTC 1 cut(s) 1352
Psp124BI GAGCTC 3 cut(s) 65, 73, 1843
Psp6I CCWGG 3 cut(s) 519, 1171, 1342
PspCI CACGTG 1 cut(s) 1016
PspFI CCCAGC 1 cut(s) 1636
PspGI CCWGG 3 cut(s) 519, 1171, 1342
PspN4I GGNNCC 3 cut(s) 498, 1341, 1348
PspPI GGNCC 3 cut(s) 497, 1347, 1813
PsrI GAACNNNNNNTAC 2 cut(s) 1641, 1673
PstNI CAGNNNCTG 2 cut(s) 58, 853
PsuI RGATCY 5 cut(s) 796, 1162, 1579, 1627, 2014
PsyI GACNNNGTC 1 cut(s) 425
PvuII CAGCTG 1 cut(s) 695
RruI TCGCGA 1 cut(s) 67
RsaI GTAC 2 cut(s) 302, 2010
RsaNI GTAC 2 cut(s) 301, 2009
SacI GAGCTC 3 cut(s) 65, 73, 1843
SapI GCTCTTC 1 cut(s) 1914
SaqAI TTAA 9 cut(s) 510, 756, 830, 1071, 1103, 1262, 1422, 1449, 1671
Sau3AI GATC 7 cut(s) 796, 967, 1162, 1579, 1627, 1951, 2014
Sau96I GGNCC 3 cut(s) 497, 1347, 1813
SchI GAGTC 4 cut(s) 1122, 1513, 1935, 2045
ScrFI CCNGG 3 cut(s) 521, 1173, 1344
SduI GDGCHC 6 cut(s) 65, 73, 112, 466, 1055, 1843
SfaNI GCATC 5 cut(s) 99, 846, 1258, 1539, 2010
SfcI CTRYAG 2 cut(s) 845, 1767
SfuI TTCGAA 3 cut(s) 176, 270, 1429
SinI GGWCC 2 cut(s) 1347, 1813
SmlI CTYRAG 2 cut(s) 1521, 1565
SmoI CTYRAG 2 cut(s) 1521, 1565
SspI AATATT 1 cut(s) 937
SspMI CTAG 5 cut(s) 243, 653, 1022, 1139, 1700
SstI GAGCTC 3 cut(s) 65, 73, 1843
StyD4I CCNGG 3 cut(s) 519, 1171, 1342
StyI CCWWGG 3 cut(s) 252, 411, 1156
TaaI ACNGT 8 cut(s) 79, 305, 316, 328, 976, 1108, 1413, 2005
TaiI ACGT 4 cut(s) 429, 594, 1018, 1648
TaqI TCGA 8 cut(s) 123, 176, 189, 264, 270, 365, 621, 1429
TaqII GACCGA 1 cut(s) 1364
TatI WGTACW 1 cut(s) 300
TauI GCSGC 6 cut(s) 185, 212, 251, 772, 1132, 1699
TfiI GAWTC 6 cut(s) 165, 335, 656, 734, 1379, 1901
Tru1I TTAA 9 cut(s) 510, 756, 830, 1071, 1103, 1262, 1422, 1449, 1671
Tru9I TTAA 9 cut(s) 510, 756, 830, 1071, 1103, 1262, 1422, 1449, 1671
TscAI CASTG 4 cut(s) 82, 1123, 1825, 1983
TseFI GTSAC 1 cut(s) 1732
TseI GCWGC 8 cut(s) 32, 259, 469, 692, 695, 1121, 1546, 1746
Tsp45I GTSAC 1 cut(s) 1732
TspDTI ATGAA 9 cut(s) 17, 456, 496, 578, 582, 813, 897, 1840, 1875
TspGWI ACGGA 4 cut(s) 27, 1466, 1496, 1910
TspRI CASTG 4 cut(s) 82, 1123, 1825, 1983
Tth111I GACNNNGTC 1 cut(s) 425
Van91I CCANNNNNTGG 1 cut(s) 1172
VneI GTGCAC 1 cut(s) 1051
VpaK11BI GGWCC 2 cut(s) 1347, 1813
XagI CCTNNNNNAGG 1 cut(s) 1658
XapI RAATTY 8 cut(s) 4, 672, 884, 981, 1142, 1257, 1559, 1866
XmiI GTMKAC 6 cut(s) 323, 407, 714, 844, 1461, 1770
XmnI GAANNNNTTC 2 cut(s) 945, 1866
XspI CTAG 5 cut(s) 243, 653, 1022, 1139, 1700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.