Rh6AG446900

aarF domain-containing protein kinase At4g31390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0001871
Physical Location & Seq
Forward (+)
63354328 .. 63367319
12992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG446900.1

Sequence Viewer

Length: 4164 bp
ATGAATTCCGTTTCTGTAAACTGCACGCGCACAGCTCCGTTTCCCGCCAAGCATCTGAGCTCCGCCGGAGCTCGTAGTGTTGCCGGTCAAATAAATGGACCGTCGGAAGGTTGGAGTTCGTTTGATTACAGGCGAAGAGTCCAGAGAAGCGGGAGGTTCCGAGTTTCGAATGCCGCGACCGGAACGCGGCGGAGCTTGGCATCTTCGAGCGACGGCGAGATCGGTTTTGGGAAGCTGAGCAAGTCTAGCGCCGCCATGGAGCAGCTCGACTTCGAGCGCGGCGTTTGCGTGCCGTTTCGCAAGTATACTCCCGAGACTGTGAGGAATAAGGTGTTGGAATCCAATGGGGCAGTTGCATCGCTAATTTCGAGAGGTGTGGAGATAGTGTGGACTTTGGGCTTGTATTGGTCTACCTTGATGTATGACTGCTTCGTCGGAAGGGATCAAGAAGTTGTTCCAAAGCGTGCTCGACAGCTTAGAAACCTCCTGTGTGATTTGGGGCCTTCTTTCATCAAAGCTGGCCAGGTTCTTGCAAACAGACCTGATATTATCAGAGAAGATTACATGAATGAACTTTGCATTCTCCAAGATGATGTTCCTCCGTTCCCCAATCAGGTCGCCTTTGACATAATAGAAGAGGAGTTGGGTCAACCCCTTGAAGCTGTATTCAGTAAGATTTCGCCACAGACAATAGCAGCTGCCAGTTTGGGTCAAGTTTACCGAGCTACTTTACGTGACACTGGCGAGGATGTAGCTATTAAGGTACAAAGGCCAGAGATAGAACCTATAATTTATAGGGATCTTTTTCTGTTCCGTACTCTTGCTTCATTCTTAAATGGAATCAGTCTACAGAAACTTGGGTGCAATGCTGAGCTCATTGTTGATGAATTCGGTGAGAAGCTTTTGGAGGAGCTTGATTACACTTTGGAAGCCCGCAATATTGAAGATTTTCTTGAGAACTTTAAAGATGATCCTACTGTCAAGATACCTGACGTTTACAAACAGCTTTCTGGTCCACGTGTTCTAGTAATGGAGTGGATCGATGGTGTTCGGTGCACTAACCCACAGGCAATTAGAGAAGCCGGTATTGATGTAAATGGGTTCTTAACTGTTGGAGTCAGTGCTGCGTTGCGCCAATTACTGGAATTTGGATTGTTCCATGGAGATCCACATCCTGGAAATGTTTTTGCTATGCGTGATGGACGTATTGGATATGTGGACTTTGGGAATGTTGCTGTGCTTAGTCAGCAAAACAAACAGATTTTGATCGATGCTGTCGTCCATGCTGTAAATGAGGACTATGTTGAGATGGCAAATGATTTCACCAGACTGGGCTTCTTGGCTCCTGGGACTGATGTCTCTCCTATTGTTCCAGCTTTAGAAGCAATCTGGCAGAATTCTCTTGGAAAAGGACTGTCTGACTTTAATTTTCGAAGTGTTACTGGGAAGTTTAATGAATTAGTTTACAACTATCCCATTCGTATTCCAGAGAGGTTTTCTCTTGTTATCAGATCTTTATTGACTCAAGAGGGTATCTGTTTTACTTTGCAGCCAGACTTCAAATTTCTTGAGGTTGCATATCCATATGTGGCAAAACGCCTCCTAACAGATCCAAACCCGGCTCTGCGTGAACGCCTCATACAAGTACTATTTAAGGATGGTGTTTTCCAATGGAAGCGGCTAGAAAATCTCATTGTTCTTGCAAAAGAAAATGTGTCCATGATGAGCAGCAATCCTGCATTGCAAGCGAAAGAAATGCAAGGGTTAAGAGAGTTGCAAGTTAGCAAGAAACTGGACCTCACTGACACCATCAAAGATGGAGCTCGCCTTTTCATTTTTGACGAAGGAATCCGTAGAAAGCTCATTCTTGCTTTGACCGAAGATTCAAAGCTTCACATAGAAGAGCTCGTGGATGTGTATAGACTGGTTGAAGATCAGATAGACATACCAACGGTTGCTGTGGAAGTAGCACGAGGCAAAGAACCTGAGTTAAAGCAGGAAACTGTAAGGCGTGATGTTTGGCGTTGTTCCATTTGTACATATGATAATGAAGAAGGTTTGACTGCGTGTGACATTTGTGGGGTTCTTCGTAAACCTTTGGGTAGAGTTGGAAGCAATATTGATAAGAAAACAGTTGAAGGCAGATGCAAAGATTCTGGAGCATCCGTAATGGCCAAGTGTCTTTTTGCATCGTTGCCGCGCAAGATAACCCAAAAGGCTGTATTATTTCATCAGCAGAAAGATGATTTTCTGATAGAAGAGGGAAATAAGTTCTACAAGCTTGGGGATATCCAAGGACCCATTCATGAATTCCAAAACACTTTTATTATTCATAAGAATGATGATATTAATATAGCTCCTTTCAAGTTTGATGTTCCATCCCCAGATGATGTGGTTTCCAACGGATTGCGATCCTCCAAAGTGGGTTCAAAAGCCAGTGTTAGTGACGTAGAGTCTTCGGGAGTTTCCTCGAAAGCTGCTAAGAAGAATAGTGCAGTTAGGGCACAATCAAGTGCTGAATGGTCAGATAGCTCATCTACTGCAGCACAAAAAGACCAACAAGCTAGATTAGATAAGGGAATTTCATCAGGGAATAGGGCATTTAGCCATCCCCATGGAAGTAGTGGCACCACAACTATACAATCAAATGGTAGTTTGTCGGACAGTTCATCCTTGCAGCCAACAAGCTCTCATATGGAAGAGGGCATTAGTTCTTTAGTGATTGGAGGCAAGCCTGAGAGTCTTACTACTAGCTCTAAGAACATGTCTTCCGATGCTAGATCTGGGCATTCGAAAATTGTTAGTTCTAGAGGATCGCATTCACGAGCAGACTATAAACCTGAAAAATGGATGCTTCCTGACGAAGCTGATGATACATTGACTCAAATGAATCTTGCAATTGTTGGACATGTTGATTCAGGAAAATCAACACTCTCAGGTAGACTGCTTCACCTTTTGGGACGGGTATCTAAAAAAGACATGCACAAATATGAGAAAGAGGCAAAGTCACAGGGCAAGGGGTCGTTTGCATATGCTTGGGCATTGGATGAGAGTGCAGAAGAAAGGGAAAGAGGAATAACTATGAATGTGGCTGTTGCATATTTTGATTCCAGAAAGTATCATGTTGTTGTGCTGGATTCTCCTGGCCATAAAGACTTTGTTCCAAACATGATATCTGGGGCAACACAAGCTGATGCTGCAATACTTCTTGTAGATGCTTCGTGTGGTGCATTTGAAGCTGGTATAAAGGGACAGACTATGGAACATGCACAACTTATCAGAAGCTTTGGTGTTGATCAAATTATAGTTGCGGTTAACAAGATGGATGTTGTTGAATACTCCAAGGATCGATTTGATGAAATTAAACAGACACTTGGAACATTTCTCCATTCCCGTGGCTTCAAAGATTCTTCAGTGTATTGGGTTCCATTGAGTGTCATGGAAAATCAAAATTTGGTATCAGCTCCTTCTGAGGCTCGTTTGTTGTCTTGGTATCGTGGACCTTCCCTGTTGGATTCAATTGATTCCCTTCAACCTCCCACGAGAGATTTTCAAAGGCCACTGCTCATGCCAATATGTGATGTCATTAGATCTACTTCTCTGGGACAGGTGTCTGCTTGTGGTAAATTGGAAGCAGGAGCTCTTCGGAGTGGATCAAAGGTTCTGGTTATGCCTTCAGGAGAATTAGGGACTGTCCGCTCTTTAGAACGGGATTCTCAGGCATGTTCCATTGCAAGAGCTGGTGACAATGTGGGTGTTACTCTTCAAGGCATTGATGGTGGTCATGTGATGGCAGGGGGCGTGCTCTGTCACCCTGGTTTTCCGGTTGCAGTTGCAAAACATTTGGAATTGAAAGTGCTTCTTTTGGATGTCGCCACCCCTATTTTAATTGGCTCTCAGGTGGAATTTCACATACACCACGCCAAGGAGGCTGCAACAGTTGTAAAAATATTGTCATTAATTGACCCAAAGGGAAAAGTGACGAAAAAGTCTCCTCGCTGTCTACTTGCAAAGCAGAGGGCTGTTATAGAGGTAGTTTTGCAAGGACCAGTTTGTGTGGAAGAGTATACAAGTTCCAAAGCTCTAGGGAGCGTATCTCTGAGGGCATCAGGAAACACTGTGGCCCTCGGTGTTGTAAGCCGGATTATAAAGGAGCAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006464 GO:0006468 GO:0006725 GO:0006778 GO:0006787 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0006995 GO:0006996 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009267 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009637 GO:0009639 GO:0009657 GO:0009658 GO:0009668 GO:0009767 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0009987 GO:0009991 GO:0010027 GO:0010033 GO:0010035 GO:0010109 GO:0010114 GO:0010287 GO:0010380 GO:0015979 GO:0015994 GO:0015996 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019216 GO:0019222 GO:0019439 GO:0019538 GO:0019684 GO:0019747 GO:0022900 GO:0031279 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031537 GO:0031540 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033015 GO:0033554 GO:0034641 GO:0036211 GO:0042221 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043562 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044270 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046149 GO:0046483 GO:0046700 GO:0046889 GO:0046890 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051186 GO:0051187 GO:0051193 GO:0051194 GO:0051716 GO:0055114 GO:0061024 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080177 GO:0080183 GO:0090056 GO:0140096 GO:1901360 GO:1901361 GO:1901401 GO:1901403 GO:1901463 GO:1901465 GO:1901562 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902171 GO:1902326 GO:1904143
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

1387

Amino Acids

152.3

Weight (kDa)

6.41

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 195 - 439 3e-72 ABC1 atypical kinase-like domain
GTP_EFTU PF00009 963 - 1177 8.4e-48 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 1207 - 1270 9.4e-09 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 1278 - 1382 4.2e-10 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 1282 - 1379 7.4e-24 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4148
AasI GACNNNNNNGTC 2 cut(s) 431, 3988
AccB1I GGYRCC 1 cut(s) 2627
AccB7I CCANNNNNTGG 2 cut(s) 1141, 1175
AccBSI CCGCTC 1 cut(s) 3699
AccI GTMKAC 6 cut(s) 305, 410, 847, 2941, 4003, 4067
AccII CGCG 5 cut(s) 28, 176, 187, 279, 2200
AcoI YGGCCR 3 cut(s) 520, 2172, 3145
AcsI RAATTY 9 cut(s) 4, 887, 1145, 1396, 1562, 2309, 2580, 3451, 3905
AcuI CTGAAG 2 cut(s) 3396, 3660
AcvI CACGTG 1 cut(s) 1019
AfaI GTAC 4 cut(s) 765, 817, 1647, 2038
AfiI CCNNNNNNNGG 6 cut(s) 107, 186, 613, 1141, 1175, 3925
AflIII ACRYGT 3 cut(s) 1018, 2763, 2908
AhdI GACNNNNNGTC 1 cut(s) 2452
AjnI CCWGG 5 cut(s) 522, 1174, 1345, 3142, 3814
AjuI GAANNNNNNNTTGG 6 cut(s) 317, 349, 579, 611, 2286, 2318
AloI GAACNNNNNNTCC 4 cut(s) 2753, 2785, 3645, 3677
Alw21I GWGCWC 9 cut(s) 62, 73, 469, 876, 1058, 1825, 1908, 3643, 3807
Alw26I GTCTC 3 cut(s) 308, 1363, 3996
Alw44I GTGCAC 1 cut(s) 1054
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 7 cut(s) 500, 520, 770, 2172, 3145, 3557, 4122
ApaLI GTGCAC 1 cut(s) 1054
ApoI RAATTY 9 cut(s) 4, 887, 1145, 1396, 1562, 2309, 2580, 3451, 3905
AseI ATTAAT 2 cut(s) 2349, 3959
Asp700I GAANNNNTTC 2 cut(s) 453, 948
AspLEI GCGC 5 cut(s) 30, 251, 279, 1134, 2202
AspS9I GGNCC 8 cut(s) 98, 500, 1013, 1795, 2297, 3500, 4046, 4123
AsuC2I CCSGG 1 cut(s) 1619
AsuHPI GGTGA 5 cut(s) 905, 1315, 2942, 3755, 3803
AsuII TTCGAA 3 cut(s) 167, 1432, 2792
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 6 cut(s) 98, 1013, 1795, 2297, 3500, 4046
BaeGI GKGCMC 2 cut(s) 1058, 2506
BalI TGGCCA 3 cut(s) 522, 2174, 3147
BanI GGYRCC 1 cut(s) 2627
BanII GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3643
BarI GAAGNNNNNNTAC 4 cut(s) 413, 445, 808, 840
BauI CACGAG 4 cut(s) 1907, 1971, 2823, 3541
BbrPI CACGTG 1 cut(s) 1019
BbsI GAAGAC 2 cut(s) 2448, 2760
Bbv12I GWGCWC 9 cut(s) 62, 73, 469, 876, 1058, 1825, 1908, 3643, 3807
BceAI ACGGC 2 cut(s) 229, 277
BcgI CGANNNNNNTGC 4 cut(s) 339, 373, 1738, 1772
BciT130I CCWGG 5 cut(s) 524, 1176, 1347, 3144, 3816
BclI TGATCA 1 cut(s) 3295
BcnI CCSGG 1 cut(s) 1619
BcoDI GTCTC 3 cut(s) 308, 1363, 3996
BfaI CTAG 8 cut(s) 246, 1025, 1682, 2565, 2751, 2778, 2808, 4085
BfmI CTRYAG 2 cut(s) 848, 2541
BfoI RGCGCY 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 3929
BglII AGATCT 3 cut(s) 1511, 2780, 3590
BlpI GCTNAGC 2 cut(s) 236, 870
BmcAI AGTACT 1 cut(s) 1647
Bme1390I CCNGG 6 cut(s) 524, 1176, 1347, 1619, 3144, 3816
Bme18I GGWCC 6 cut(s) 98, 1013, 1795, 2297, 3500, 4046
BmeRI GACNNNNNGTC 1 cut(s) 2452
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 8 cut(s) 98, 500, 1013, 1795, 2297, 3500, 4046, 4123
BmiI GGNNCC 6 cut(s) 158, 501, 1344, 2299, 2629, 3426
BmrFI CCNGG 6 cut(s) 524, 1176, 1347, 1619, 3144, 3816
BmrI ACTGGG 2 cut(s) 1340, 1452
BmuI ACTGGG 2 cut(s) 1340, 1452
BoxI GACNNNNGTC 2 cut(s) 1355, 3610
BpiI GAAGAC 2 cut(s) 2448, 2760
BplI GAGNNNNNCTC 4 cut(s) 1483, 1515, 4081, 4113
BpmI CTGGAG 1 cut(s) 2178
Bpu1102I GCTNAGC 2 cut(s) 236, 870
Bpu14I TTCGAA 3 cut(s) 167, 1432, 2792
BpuEI CTTGAG 3 cut(s) 974, 1509, 1589
BpuMI CCSGG 1 cut(s) 1619
Bsa29I ATCGAT 3 cut(s) 1041, 1269, 3349
BsaAI YACGTR 2 cut(s) 734, 1019
BsaBI GATNNNNATC 3 cut(s) 1170, 1265, 2410
BsaWI WCCGGW 2 cut(s) 179, 3823
BsaXI ACNNNNNCTCC 5 cut(s) 371, 401, 2151, 2181, 4145
Bsc4I CCNNNNNNNGG 6 cut(s) 107, 186, 613, 1141, 1175, 3925
Bse118I RCCGGY 2 cut(s) 83, 1082
Bse1I ACTGG 9 cut(s) 702, 745, 1146, 1335, 1447, 1797, 1929, 2436, 4049
Bse3DI GCAATG 3 cut(s) 871, 1739, 3729
Bse8I GATNNNNATC 3 cut(s) 1170, 1265, 2410
BseBI CCWGG 5 cut(s) 524, 1176, 1347, 3144, 3816
BseCI ATCGAT 3 cut(s) 1041, 1269, 3349
BseJI GATNNNNATC 3 cut(s) 1170, 1265, 2410
BseLI CCNNNNNNNGG 6 cut(s) 107, 186, 613, 1141, 1175, 3925
BseMI GCAATG 3 cut(s) 871, 1739, 3729
BseNI ACTGG 9 cut(s) 702, 745, 1146, 1335, 1447, 1797, 1929, 2436, 4049
BseRI GAGGAG 3 cut(s) 653, 923, 3984
BseSI GKGCMC 2 cut(s) 1058, 2506
BsgI GTGCAG 3 cut(s) 7, 2514, 3075
Bsh1236I CGCG 5 cut(s) 28, 176, 187, 279, 2200
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 7 cut(s) 502, 522, 772, 2174, 3147, 3559, 4124
BshNI GGYRCC 1 cut(s) 2627
BshVI ATCGAT 3 cut(s) 1041, 1269, 3349
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 9 cut(s) 62, 73, 469, 876, 1058, 1825, 1908, 3643, 3807
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 7 cut(s) 66, 84, 180, 1083, 1619, 3824, 4141
BslFI GGGAC 5 cut(s) 1363, 2973, 3264, 3618, 3703
BslI CCNNNNNNNGG 6 cut(s) 107, 186, 613, 1141, 1175, 3925
BsmAI GTCTC 3 cut(s) 308, 1363, 3996
BsmFI GGGAC 5 cut(s) 1363, 2973, 3264, 3618, 3703
BsmI GAATGC 4 cut(s) 175, 579, 2788, 2818
BsnI GGCC 7 cut(s) 502, 522, 772, 2174, 3147, 3559, 4124
BsoBI CYCGRG 1 cut(s) 311
Bsp119I TTCGAA 3 cut(s) 167, 1432, 2792
Bsp1407I TGTACA 1 cut(s) 2036
Bsp1720I GCTNAGC 2 cut(s) 236, 870
Bsp19I CCATGG 3 cut(s) 255, 1159, 2614
BspANI GGCC 7 cut(s) 502, 522, 772, 2174, 3147, 3559, 4124
BspDI ATCGAT 3 cut(s) 1041, 1269, 3349
BspFNI CGCG 5 cut(s) 28, 176, 187, 279, 2200
BspHI TCATGA 1 cut(s) 2305
BspLI GGNNCC 6 cut(s) 158, 501, 1344, 2299, 2629, 3426
BspMAI CTGCAG 1 cut(s) 2545
BspQI GCTCTTC 2 cut(s) 1896, 3648
BspT104I TTCGAA 3 cut(s) 167, 1432, 2792
BspT107I GGYRCC 1 cut(s) 2627
BsrBI CCGCTC 1 cut(s) 3699
BsrDI GCAATG 3 cut(s) 871, 1739, 3729
BsrFI RCCGGY 2 cut(s) 83, 1082
BsrGI TGTACA 1 cut(s) 2036
BsrI ACTGG 9 cut(s) 702, 745, 1146, 1335, 1447, 1797, 1929, 2436, 4049
BssAI RCCGGY 2 cut(s) 83, 1082
BssNAI GTATAC 2 cut(s) 306, 4068
BssSI CACGAG 4 cut(s) 1907, 1971, 2823, 3541
BssT1I CCWWGG 6 cut(s) 255, 1159, 2293, 2614, 3342, 3924
Bst1107I GTATAC 2 cut(s) 306, 4068
Bst2BI CACGAG 4 cut(s) 1907, 1971, 2823, 3541
Bst2UI CCWGG 5 cut(s) 524, 1176, 1347, 3144, 3816
Bst6I CTCTTC 8 cut(s) 130, 630, 1896, 2253, 2694, 3648, 3768, 4056
BstAUI TGTACA 1 cut(s) 2036
BstBAI YACGTR 2 cut(s) 734, 1019
BstBI TTCGAA 3 cut(s) 167, 1432, 2792
BstC8I GCNNGC 9 cut(s) 26, 290, 465, 520, 934, 1746, 1825, 2732, 3803
BstDSI CCRYGG 4 cut(s) 255, 1159, 2614, 3394
BstFNI CGCG 5 cut(s) 28, 176, 187, 279, 2200
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 5 cut(s) 30, 251, 279, 1134, 2202
BstMAI GTCTC 3 cut(s) 308, 1363, 3996
BstMCI CGRYCG 1 cut(s) 180
BstNI CCWGG 5 cut(s) 524, 1176, 1347, 3144, 3816
BstNSI RCATGY 5 cut(s) 2767, 2912, 2983, 3269, 3726
BstPAI GACNNNNGTC 2 cut(s) 1355, 3610
BstSCI CCNGG 6 cut(s) 522, 1174, 1345, 1617, 3142, 3814
BstSFI CTRYAG 2 cut(s) 848, 2541
BstSLI GKGCMC 2 cut(s) 1058, 2506
BstUI CGCG 5 cut(s) 28, 176, 187, 279, 2200
BstV2I GAAGAC 2 cut(s) 2448, 2760
BstX2I RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2780, 3590
BstXI CCANNNNNNTGG 2 cut(s) 2615, 3395
BstYI RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2780, 3590
BstZ17I GTATAC 2 cut(s) 306, 4068
Bsu15I ATCGAT 3 cut(s) 1041, 1269, 3349
BsuRI GGCC 7 cut(s) 502, 522, 772, 2174, 3147, 3559, 4124
BsuTUI ATCGAT 3 cut(s) 1041, 1269, 3349
BtgI CCRYGG 4 cut(s) 255, 1159, 2614, 3394
BtgZI GCGATG 1 cut(s) 342
BtsI GCAGTG 1 cut(s) 3560
BtsIMutI CAGTG 7 cut(s) 738, 1126, 1800, 2443, 3420, 3560, 4116
Cac8I GCNNGC 9 cut(s) 26, 290, 465, 520, 934, 1746, 1825, 2732, 3803
CciI TCATGA 1 cut(s) 2305
CfoI GCGC 5 cut(s) 30, 251, 279, 1134, 2202
Cfr10I RCCGGY 2 cut(s) 83, 1082
Cfr13I GGNCC 8 cut(s) 98, 500, 1013, 1795, 2297, 3500, 4046, 4123
ClaI ATCGAT 3 cut(s) 1041, 1269, 3349
Csp6I GTAC 4 cut(s) 764, 816, 1646, 2037
CviQI GTAC 4 cut(s) 764, 816, 1646, 2037
DraI TTTAAA 1 cut(s) 964
DrdI GACNNNNNNGTC 2 cut(s) 431, 3988
DriI GACNNNNNGTC 1 cut(s) 2452
DseDI GACNNNNNNGTC 2 cut(s) 431, 3988
EaeI YGGCCR 3 cut(s) 520, 2172, 3145
Eam1104I CTCTTC 8 cut(s) 130, 630, 1896, 2253, 2694, 3648, 3768, 4056
Eam1105I GACNNNNNGTC 1 cut(s) 2452
EarI CTCTTC 8 cut(s) 130, 630, 1896, 2253, 2694, 3648, 3768, 4056
EciI GGCGGA 2 cut(s) 52, 205
Ecl136II GAGCTC 6 cut(s) 60, 71, 874, 1823, 1906, 3641
Eco130I CCWWGG 6 cut(s) 255, 1159, 2293, 2614, 3342, 3924
Eco24I GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3643
Eco32I GATATC 2 cut(s) 2290, 3174
Eco47I GGWCC 6 cut(s) 98, 1013, 1795, 2297, 3500, 4046
Eco53kI GAGCTC 6 cut(s) 60, 71, 874, 1823, 1906, 3641
Eco57I CTGAAG 2 cut(s) 3396, 3660
Eco72I CACGTG 1 cut(s) 1019
Eco88I CYCGRG 1 cut(s) 311
EcoICRI GAGCTC 6 cut(s) 60, 71, 874, 1823, 1906, 3641
EcoO109I RGGNCCY 2 cut(s) 500, 2297
EcoRI GAATTC 4 cut(s) 4, 887, 1396, 2309
EcoRII CCWGG 5 cut(s) 522, 1174, 1345, 3142, 3814
EcoRV GATATC 2 cut(s) 2290, 3174
EcoT14I CCWWGG 6 cut(s) 255, 1159, 2293, 2614, 3342, 3924
EcoT38I GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3643
ErhI CCWWGG 6 cut(s) 255, 1159, 2293, 2614, 3342, 3924
FalI AAGNNNNNCTT 4 cut(s) 936, 968, 3846, 3878
FaqI GGGAC 5 cut(s) 1363, 2973, 3264, 3618, 3703
FauI CCCGC 3 cut(s) 52, 143, 941
FauNDI CATATG 4 cut(s) 1585, 2041, 2694, 3031
FbaI TGATCA 1 cut(s) 3295
FblI GTMKAC 6 cut(s) 305, 410, 847, 2941, 4003, 4067
FriOI GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3643
FspBI CTAG 8 cut(s) 246, 1025, 1682, 2565, 2751, 2778, 2808, 4085
GlaI GCGC 5 cut(s) 29, 250, 278, 1133, 2201
GsuI CTGGAG 1 cut(s) 2178
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 7 cut(s) 502, 522, 772, 2174, 3147, 3559, 4124
HapII CCGG 7 cut(s) 66, 84, 180, 1083, 1619, 3824, 4141
HhaI GCGC 5 cut(s) 30, 251, 279, 1134, 2202
Hin6I GCGC 5 cut(s) 28, 249, 277, 1132, 2200
HinP1I GCGC 5 cut(s) 28, 249, 277, 1132, 2200
HincII GTYRAC 2 cut(s) 650, 3316
HindII GTYRAC 2 cut(s) 650, 3316
HindIII AAGCTT 4 cut(s) 899, 1889, 2279, 3283
HpaI GTTAAC 1 cut(s) 3316
HpaII CCGG 7 cut(s) 66, 84, 180, 1083, 1619, 3824, 4141
HphI GGTGA 5 cut(s) 905, 1315, 2942, 3755, 3803
Hpy99I CGWCG 3 cut(s) 106, 215, 437
HpyCH4IV ACGT 5 cut(s) 733, 993, 1018, 1204, 2448
HpySE526I ACGT 5 cut(s) 733, 993, 1018, 1204, 2448
HspAI GCGC 5 cut(s) 28, 249, 277, 1132, 2200
Ksp22I TGATCA 1 cut(s) 3295
KspAI GTTAAC 1 cut(s) 3316
LguI GCTCTTC 2 cut(s) 1896, 3648
MaeI CTAG 8 cut(s) 246, 1025, 1682, 2565, 2751, 2778, 2808, 4085
MaeII ACGT 5 cut(s) 733, 993, 1018, 1204, 2448
MaeIII GTNAC 9 cut(s) 734, 1438, 2069, 2444, 3006, 3743, 3757, 3809, 3979
MbiI CCGCTC 1 cut(s) 3699
MfeI CAATTG 2 cut(s) 2898, 3519
MflI RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2780, 3590
MlsI TGGCCA 3 cut(s) 522, 2174, 3147
MluNI TGGCCA 3 cut(s) 522, 2174, 3147
MlyI GAGTC 6 cut(s) 147, 1125, 1516, 2462, 2749, 2875
Mox20I TGGCCA 3 cut(s) 522, 2174, 3147
MroXI GAANNNNTTC 2 cut(s) 453, 948
MscI TGGCCA 3 cut(s) 522, 2174, 3147
MslI CAYNNNNRTG 4 cut(s) 2337, 2613, 2988, 3393
Msp20I TGGCCA 3 cut(s) 522, 2174, 3147
MspA1I CMGCKG 1 cut(s) 698
MspI CCGG 7 cut(s) 66, 84, 180, 1083, 1619, 3824, 4141
MspR9I CCNGG 6 cut(s) 524, 1176, 1347, 1619, 3144, 3816
MunI CAATTG 2 cut(s) 2898, 3519
Mva1269I GAATGC 4 cut(s) 175, 579, 2788, 2818
MvaI CCWGG 5 cut(s) 524, 1176, 1347, 3144, 3816
MvnI CGCG 5 cut(s) 28, 176, 187, 279, 2200
NciI CCSGG 1 cut(s) 1619
NcoI CCATGG 3 cut(s) 255, 1159, 2614
NdeI CATATG 4 cut(s) 1585, 2041, 2694, 3031
NlaIV GGNNCC 6 cut(s) 158, 501, 1344, 2299, 2629, 3426
NmuCI GTSAC 7 cut(s) 734, 2069, 2444, 3006, 3743, 3809, 3979
NspI RCATGY 5 cut(s) 2767, 2912, 2983, 3269, 3726
NspV TTCGAA 3 cut(s) 167, 1432, 2792
PagI TCATGA 1 cut(s) 2305
PciI ACATGT 2 cut(s) 2763, 2908
PciSI GCTCTTC 2 cut(s) 1896, 3648
PcsI WCGNNNNNNNCGW 4 cut(s) 173, 279, 365, 1275
PctI GAATGC 4 cut(s) 175, 579, 2788, 2818
PdmI GAANNNNTTC 2 cut(s) 453, 948
PflMI CCANNNNNTGG 2 cut(s) 1141, 1175
PfoI TCCNGGA 1 cut(s) 1174
PleI GAGTC 6 cut(s) 146, 1124, 1516, 2461, 2748, 2875
PmaCI CACGTG 1 cut(s) 1019
PmlI CACGTG 1 cut(s) 1019
PpsI GAGTC 6 cut(s) 146, 1124, 1516, 2461, 2748, 2875
Ppu21I YACGTR 2 cut(s) 734, 1019
PpuMI RGGWCCY 1 cut(s) 2297
PscI ACATGT 2 cut(s) 2763, 2908
PshAI GACNNNNGTC 2 cut(s) 1355, 3610
PshBI ATTAAT 2 cut(s) 2349, 3959
PsiI TTATAA 1 cut(s) 4148
Psp124BI GAGCTC 6 cut(s) 62, 73, 876, 1825, 1908, 3643
Psp5II RGGWCCY 1 cut(s) 2297
Psp6I CCWGG 5 cut(s) 522, 1174, 1345, 3142, 3814
PspCI CACGTG 1 cut(s) 1019
PspGI CCWGG 5 cut(s) 522, 1174, 1345, 3142, 3814
PspN4I GGNNCC 6 cut(s) 158, 501, 1344, 2299, 2629, 3426
PspPI GGNCC 8 cut(s) 98, 500, 1013, 1795, 2297, 3500, 4046, 4123
PspPPI RGGWCCY 1 cut(s) 2297
PsrI GAACNNNNNNTAC 2 cut(s) 1623, 1655
PstI CTGCAG 1 cut(s) 2545
PsuI RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2780, 3590
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 4 cut(s) 765, 817, 1647, 2038
RsaNI GTAC 4 cut(s) 764, 816, 1646, 2037
RseI CAYNNNNRTG 4 cut(s) 2337, 2613, 2988, 3393
SacI GAGCTC 6 cut(s) 62, 73, 876, 1825, 1908, 3643
SapI GCTCTTC 2 cut(s) 1896, 3648
Sau96I GGNCC 8 cut(s) 98, 500, 1013, 1795, 2297, 3500, 4046, 4123
ScaI AGTACT 1 cut(s) 1647
SchI GAGTC 6 cut(s) 147, 1125, 1516, 2462, 2749, 2875
ScrFI CCNGG 6 cut(s) 524, 1176, 1347, 1619, 3144, 3816
SfcI CTRYAG 2 cut(s) 848, 2541
SfuI TTCGAA 3 cut(s) 167, 1432, 2792
SinI GGWCC 6 cut(s) 98, 1013, 1795, 2297, 3500, 4046
SmiMI CAYNNNNRTG 4 cut(s) 2337, 2613, 2988, 3393
SmlI CTYRAG 3 cut(s) 953, 1524, 1568
SmoI CTYRAG 3 cut(s) 953, 1524, 1568
SspI AATATT 3 cut(s) 940, 2119, 3951
SspMI CTAG 8 cut(s) 246, 1025, 1682, 2565, 2751, 2778, 2808, 4085
SstI GAGCTC 6 cut(s) 62, 73, 876, 1825, 1908, 3643
StyD4I CCNGG 6 cut(s) 522, 1174, 1345, 1617, 3142, 3814
StyI CCWWGG 6 cut(s) 255, 1159, 2293, 2614, 3342, 3924
TaiI ACGT 5 cut(s) 736, 996, 1021, 1207, 2451
TaqII GACCGA 1 cut(s) 1892
TatI WGTACW 2 cut(s) 1645, 2036
TauI GCSGC 6 cut(s) 176, 190, 254, 282, 1681, 2200
TscAI CASTG 7 cut(s) 745, 1126, 1807, 2443, 3420, 3567, 4123
TseFI GTSAC 7 cut(s) 734, 2069, 2444, 3006, 3743, 3809, 3979
Tsp45I GTSAC 7 cut(s) 734, 2069, 2444, 3006, 3743, 3809, 3979
TspGWI ACGGA 6 cut(s) 27, 591, 803, 1841, 2155, 2418
TspRI CASTG 7 cut(s) 745, 1126, 1807, 2443, 3420, 3567, 4123
Van91I CCANNNNNTGG 2 cut(s) 1141, 1175
VneI GTGCAC 1 cut(s) 1054
VpaK11BI GGWCC 6 cut(s) 98, 1013, 1795, 2297, 3500, 4046
VspI ATTAAT 2 cut(s) 2349, 3959
XapI RAATTY 9 cut(s) 4, 887, 1145, 1396, 1562, 2309, 2580, 3451, 3905
XbaI TCTAGA 1 cut(s) 2807
XceI RCATGY 5 cut(s) 2767, 2912, 2983, 3269, 3726
XcmI CCANNNNNNNNNTGG 1 cut(s) 2621
XmiI GTMKAC 6 cut(s) 305, 410, 847, 2941, 4003, 4067
XmnI GAANNNNTTC 2 cut(s) 453, 948
XspI CTAG 8 cut(s) 246, 1025, 1682, 2565, 2751, 2778, 2808, 4085
ZrmI AGTACT 1 cut(s) 1647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.