MD15G1109100.v1.1

aarF domain-containing protein kinase At4g31390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001871
Physical Location & Seq
Reverse (-)
7605806 .. 7611855
6050 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1109100.v1.1.491

Sequence Viewer

Length: 2037 bp
ATGAATTTGCTCTGCGTAAATTGCGTAACCGCGACTCCGTTCCCTGCCAATCAGCATCTGAGCTCGCGAGCTCACACTGTGCCGAGCCGTAGAAATGCGCCGTCCAAGGCTCCGGCGTCGTTTGATTACAGGCTCCGAATGCGAGGAGGCAAGAAACCGTTGCGATTCCGAGTTTCTAATGCCGCAATCGAAACGCGGAGGAGCTTGGCGGCGTCTGCGGGAGAAGGGCAATTGACCAAACCTAGCGCCGCCATGGAGCAGCTCGACTTCGAACGCGGCGTTTGCGTGCCGTTTCGCAAGTACAGTCCAGAAACTGTGAGAAATACGGTGTTGAAATCCAGAGGAGCAATTGTGTCGTTACTTTCGAGGGGCGTGGAGATTGTGTGGAGTTTGGGATTGTACTGGTCTACCTTGGCGTATGACTACTTCGTAGGAAGGGACGAAGAAGTTGTTTCAGATCGTGCTCGGCAGCTTAGAAACCTCCTATGTGATTTGGGGCCTTCTTTCATTAAAGCCGGCCAGGTTCTCGCAAACAGACCTGATATTATCAGAGAAGATTATATGAACGAACTGTGCATTCTTCAAGATGACGTTCCTCCCTTCCCCAATCAGGTTGCCTTCGACATAATAGAAGAAGAAATGGGGCGACCCCTCGAAGCTGTTTTCAGTAAAATTTCTTCTCAGACGATAGCAGCTGCCAGTTTAGGTCAAGTATACCGAGCTACGTTACGTGACTCTGGCAAGGAAGTTGCTATTAAGGTTCAGAGGCCGCAAATAGAACCTATAATTTATCGGGATCTTTTTCTTTTCCGAACTCTTGCTTCATTCTTAAATGGCATCAGTCTGCAGAAACTGGGGTGCAATGCTGAGCTGATTGTTGATGAATTTGGTGAGAAGCTTTTGGAGGAGCTTGATTACACTTTGGAAGCCCGTAATATTGAAGATTTTCTTCTGAACTTTGAAGGTGATCCTACCGTCAAAATTCCTCTAGTTTACAAAGAGCTTTGTAGTTCACGTGTTCTAGTGATGGAGTGGATTGATGGCATTCGGTGCACTAACCCACGGGCTATTAAAGAAGCTGGTATTGATGTGAATGGGTTCTTAACGGTTGGAGTCAGTGCCGCATTGCGGCAATTACTGGAATTTGGGTTGTTCCATGGGGATCCACATCCTGGAAATGTTTTTGCCATGCGTGATGGGCGGATTGCATATGTGGACTTTGGGAATGTTGCTGTGCTTAGTCAGCAAAATAAACAAATTTTGATTGACGCTGTTGTCCATGCTGTAAACGAGGACTATGTTGAGATGGCAAATGATTTCACCAGACTTGGCTTCTTGGCTCCTGGGACCGATGTCTCTCCTATTGTTCCAGCTTTAGAAGCGATATGGCAGAACTCTGTTGGAAAAGGACTGTCTGACTTTAATTTTCGAAGCGTTACCGGGAAGTTTAACCAATTAGTCTACAACTATCCCATCCGTATCCCGGAGAGGTTTTCCCTTGTCATCCGTTCTTTATTGACTCAAGAGGGCATCTGTTTTACTTTGCAGCCAGACTTCAAGTTTCTTGAGGTTGCCTATCCATATGTGGCAAAGCGCCTCCTGACAGATCCAAACCCAGCTCTCCGTGAACGCCTCATACAGGTACTATTTAAAGATGGTGTTTTCCAATGGAAGCGGCTAGAAAACCTTATTGTTCTGGCCAAAGAAAACGTGACCAAGATGAGCAGCAATCCTGCATTCCAAGCTGTAGACACGCAACGGTCAAGAGAATTGCAAGTTAGTAGGAAACTGGACCTCACTGACACCATCAAAGATGGAGCTCGCCTTTTCATTTTTGATGAAGGAATTCGTAGAAAGCTTATTCTTGCTTTGACGGAAGATTCAAGGCTTCACATTGAAGAGCTTGTGGATGTGTATAGACTCGTTGAAGATCAGATAGACATACCGTCAGTTGCAATGGAAGTAGCACGAGATTTCCCCAGCGTTATACGAGATCTGATGCTTTCGTGGACTGATTCGGTGTTATCTGATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006464 GO:0006468 GO:0006725 GO:0006778 GO:0006787 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0006995 GO:0006996 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009267 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009637 GO:0009639 GO:0009657 GO:0009658 GO:0009668 GO:0009767 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0009987 GO:0009991 GO:0010027 GO:0010033 GO:0010035 GO:0010109 GO:0010114 GO:0010287 GO:0010380 GO:0015979 GO:0015994 GO:0015996 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019216 GO:0019222 GO:0019439 GO:0019538 GO:0019684 GO:0019747 GO:0022900 GO:0031279 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031537 GO:0031540 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033015 GO:0033554 GO:0034641 GO:0036211 GO:0042221 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043562 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044270 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046149 GO:0046483 GO:0046700 GO:0046889 GO:0046890 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051186 GO:0051187 GO:0051193 GO:0051194 GO:0051716 GO:0055114 GO:0061024 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080177 GO:0080183 GO:0090056 GO:0140096 GO:1901360 GO:1901361 GO:1901401 GO:1901403 GO:1901463 GO:1901465 GO:1901562 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902171 GO:1902326 GO:1904143
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

679

Amino Acids

76.44

Weight (kDa)

5.82

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 194 - 438 2.9e-71 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1274
AccB7I CCANNNNNTGG 1 cut(s) 1172
AccI GTMKAC 4 cut(s) 407, 714, 1461, 1749
AccII CGCG 4 cut(s) 32, 67, 196, 276
AclWI GGATC 5 cut(s) 804, 962, 1157, 1170, 1601
AcoI YGGCCR 2 cut(s) 517, 1698
AcsI RAATTY 7 cut(s) 4, 672, 884, 981, 1142, 1257, 1845
AcvI CACGTG 1 cut(s) 1016
AcyI GRCGYC 2 cut(s) 116, 212
AdeI CACNNNGTG 1 cut(s) 79
AfaI GTAC 3 cut(s) 302, 401, 1644
AfiI CCNNNNNNNGG 5 cut(s) 610, 1128, 1172, 1483, 1639
AflIII ACRYGT 1 cut(s) 1015
AgsI TTSAA 8 cut(s) 334, 584, 941, 962, 1558, 1884, 1898, 1928
AhdI GACNNNNNGTC 1 cut(s) 1945
AjnI CCWGG 3 cut(s) 519, 1171, 1342
Alw21I GWGCWC 5 cut(s) 65, 73, 466, 1055, 1822
Alw26I GTCTC 1 cut(s) 1360
Alw44I GTGCAC 1 cut(s) 1051
AlwI GGATC 5 cut(s) 804, 962, 1157, 1170, 1601
AlwNI CAGNNNCTG 3 cut(s) 58, 314, 853
AoxI GGCC 4 cut(s) 497, 517, 767, 1698
ApaLI GTGCAC 1 cut(s) 1051
ApeKI GCWGC 6 cut(s) 259, 469, 692, 695, 1546, 1725
ApoI RAATTY 7 cut(s) 4, 672, 884, 981, 1142, 1257, 1845
Asp700I GAANNNNTTC 3 cut(s) 945, 1097, 1845
AspLEI GCGC 3 cut(s) 100, 248, 1596
AspS9I GGNCC 3 cut(s) 497, 1347, 1792
AsuC2I CCSGG 2 cut(s) 1441, 1484
AsuHPI GGTGA 3 cut(s) 902, 977, 1312
AsuII TTCGAA 2 cut(s) 270, 1429
AvaII GGWCC 2 cut(s) 1347, 1792
BaeGI GKGCMC 1 cut(s) 1055
BalI TGGCCA 1 cut(s) 1700
BamHI GGATCC 1 cut(s) 1162
BanII GRGCYC 3 cut(s) 65, 73, 1822
BarI GAAGNNNNNNTAC 4 cut(s) 410, 442, 661, 693
BauI CACGAG 1 cut(s) 1968
BbrPI CACGTG 1 cut(s) 1016
Bbv12I GWGCWC 5 cut(s) 65, 73, 466, 1055, 1822
BbvI GCAGC 6 cut(s) 271, 481, 682, 704, 1558, 1737
BccI CCATC 8 cut(s) 1021, 1034, 1190, 1300, 1481, 1649, 1808, 1814
BceAI ACGGC 3 cut(s) 72, 85, 274
BcgI CGANNNNNNTGC 2 cut(s) 336, 370
BciT130I CCWGG 3 cut(s) 521, 1173, 1344
BciVI GTATCC 1 cut(s) 1490
BcnI CCSGG 2 cut(s) 1441, 1484
BcoDI GTCTC 1 cut(s) 1360
BfaI CTAG 4 cut(s) 243, 989, 1022, 1679
BfmI CTRYAG 2 cut(s) 845, 1746
BfoI RGCGCY 2 cut(s) 249, 1597
BfuI GTATCC 1 cut(s) 1490
BglII AGATCT 1 cut(s) 1993
BlpI GCTNAGC 1 cut(s) 867
Bme1390I CCNGG 5 cut(s) 521, 1173, 1344, 1441, 1484
Bme18I GGWCC 2 cut(s) 1347, 1792
BmeRI GACNNNNNGTC 1 cut(s) 1945
BmgT120I GGNCC 3 cut(s) 497, 1347, 1792
BmiI GGNNCC 6 cut(s) 111, 134, 498, 1164, 1341, 1348
BmrFI CCNGG 5 cut(s) 521, 1173, 1344, 1441, 1484
BmrI ACTGGG 1 cut(s) 863
BmsI GCATC 4 cut(s) 64, 846, 1539, 1989
BmuI ACTGGG 1 cut(s) 863
BoxI GACNNNNGTC 1 cut(s) 1352
Bpu1102I GCTNAGC 1 cut(s) 867
Bpu14I TTCGAA 2 cut(s) 270, 1429
BpuEI CTTGAG 2 cut(s) 1506, 1586
BpuMI CCSGG 2 cut(s) 1441, 1484
BsaAI YACGTR 2 cut(s) 731, 1016
BsaBI GATNNNNATC 1 cut(s) 1167
BsaHI GRCGYC 2 cut(s) 116, 212
BsaJI CCNNGG 6 cut(s) 105, 252, 411, 1061, 1156, 1343
BsaXI ACNNNNNCTCC 6 cut(s) 19, 49, 336, 366, 368, 398
Bsc4I CCNNNNNNNGG 5 cut(s) 610, 1128, 1172, 1483, 1639
Bse118I RCCGGY 1 cut(s) 515
Bse1I ACTGG 5 cut(s) 407, 699, 858, 1143, 1794
Bse3DI GCAATG 3 cut(s) 868, 1124, 1962
Bse8I GATNNNNATC 1 cut(s) 1167
BseBI CCWGG 3 cut(s) 521, 1173, 1344
BseDI CCNNGG 6 cut(s) 105, 252, 411, 1061, 1156, 1343
BseGI GGATG 4 cut(s) 1168, 1473, 1503, 1915
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 5 cut(s) 610, 1128, 1172, 1483, 1639
BseMI GCAATG 3 cut(s) 868, 1124, 1962
BseMII CTCAG 3 cut(s) 50, 695, 858
BseNI ACTGG 5 cut(s) 407, 699, 858, 1143, 1794
BseRI GAGGAG 4 cut(s) 159, 214, 357, 920
BseSI GKGCMC 1 cut(s) 1055
BseXI GCAGC 6 cut(s) 271, 481, 682, 704, 1558, 1737
BseYI CCCAGC 2 cut(s) 1615, 1979
Bsh1236I CGCG 4 cut(s) 32, 67, 196, 276
BshFI GGCC 4 cut(s) 499, 519, 769, 1700
BsiHKAI GWGCWC 5 cut(s) 65, 73, 466, 1055, 1822
BsiSI CCGG 4 cut(s) 113, 516, 1440, 1484
BslFI GGGAC 2 cut(s) 452, 1360
BslI CCNNNNNNNGG 5 cut(s) 610, 1128, 1172, 1483, 1639
BsmAI GTCTC 1 cut(s) 1360
BsmFI GGGAC 2 cut(s) 452, 1360
BsmI GAATGC 4 cut(s) 144, 576, 1044, 1736
BsnI GGCC 4 cut(s) 499, 519, 769, 1700
Bsp119I TTCGAA 2 cut(s) 270, 1429
Bsp1286I GDGCHC 5 cut(s) 65, 73, 466, 1055, 1822
Bsp143I GATC 7 cut(s) 457, 796, 967, 1162, 1606, 1930, 1993
Bsp1720I GCTNAGC 1 cut(s) 867
Bsp19I CCATGG 2 cut(s) 252, 1156
Bsp68I TCGCGA 1 cut(s) 67
BspANI GGCC 4 cut(s) 499, 519, 769, 1700
BspCNI CTCAG 3 cut(s) 51, 694, 859
BspFNI CGCG 4 cut(s) 32, 67, 196, 276
BspLI GGNNCC 6 cut(s) 111, 134, 498, 1164, 1341, 1348
BspMAI CTGCAG 1 cut(s) 849
BspPI GGATC 5 cut(s) 804, 962, 1157, 1170, 1601
BspQI GCTCTTC 1 cut(s) 1893
BspT104I TTCGAA 2 cut(s) 270, 1429
BsrDI GCAATG 3 cut(s) 868, 1124, 1962
BsrFI RCCGGY 1 cut(s) 515
BsrI ACTGG 5 cut(s) 407, 699, 858, 1143, 1794
BssAI RCCGGY 1 cut(s) 515
BssECI CCNNGG 6 cut(s) 105, 252, 411, 1061, 1156, 1343
BssMI GATC 7 cut(s) 457, 796, 967, 1162, 1606, 1930, 1993
BssNAI GTATAC 1 cut(s) 715
BssNI GRCGYC 2 cut(s) 116, 212
BssSI CACGAG 1 cut(s) 1968
BssT1I CCWWGG 4 cut(s) 105, 252, 411, 1156
Bst1107I GTATAC 1 cut(s) 715
Bst2BI CACGAG 1 cut(s) 1968
Bst2UI CCWGG 3 cut(s) 521, 1173, 1344
Bst6I CTCTTC 1 cut(s) 1893
BstACI GRCGYC 2 cut(s) 116, 212
BstAPI GCANNNNNTGC 1 cut(s) 1050
BstBAI YACGTR 2 cut(s) 731, 1016
BstBI TTCGAA 2 cut(s) 270, 1429
BstC8I GCNNGC 5 cut(s) 65, 69, 287, 517, 1822
BstDEI CTNAG 5 cut(s) 59, 473, 681, 867, 1238
BstDSI CCRYGG 3 cut(s) 252, 1061, 1156
BstENI CCTNNNNNAGG 1 cut(s) 1637
BstF5I GGATG 4 cut(s) 1168, 1473, 1503, 1915
BstFNI CGCG 4 cut(s) 32, 67, 196, 276
BstH2I RGCGCY 2 cut(s) 249, 1597
BstHHI GCGC 3 cut(s) 100, 248, 1596
BstKTI GATC 7 cut(s) 460, 799, 970, 1165, 1609, 1933, 1996
BstMAI GTCTC 1 cut(s) 1360
BstMBI GATC 7 cut(s) 457, 796, 967, 1162, 1606, 1930, 1993
BstMWI GCNNNNNNNGC 9 cut(s) 21, 139, 215, 282, 1050, 1198, 1243, 1379, 1742
BstNI CCWGG 3 cut(s) 521, 1173, 1344
BstPAI GACNNNNGTC 1 cut(s) 1352
BstSCI CCNGG 5 cut(s) 519, 1171, 1342, 1439, 1482
BstSFI CTRYAG 2 cut(s) 845, 1746
BstSLI GKGCMC 1 cut(s) 1055
BstUI CGCG 4 cut(s) 32, 67, 196, 276
BstV1I GCAGC 6 cut(s) 271, 481, 682, 704, 1558, 1737
BstX2I RGATCY 4 cut(s) 796, 1162, 1606, 1993
BstYI RGATCY 4 cut(s) 796, 1162, 1606, 1993
BstZ17I GTATAC 1 cut(s) 715
BsuI GTATCC 1 cut(s) 1490
BsuRI GGCC 4 cut(s) 499, 519, 769, 1700
BtgI CCRYGG 3 cut(s) 252, 1061, 1156
BtsCI GGATG 4 cut(s) 1168, 1473, 1503, 1915
BtsIMutI CAGTG 3 cut(s) 75, 1123, 1797
BtuMI TCGCGA 1 cut(s) 67
Cac8I GCNNGC 5 cut(s) 65, 69, 287, 517, 1822
CaiI CAGNNNCTG 3 cut(s) 58, 314, 853
CfoI GCGC 3 cut(s) 100, 248, 1596
Cfr10I RCCGGY 1 cut(s) 515
Cfr13I GGNCC 3 cut(s) 497, 1347, 1792
CseI GACGC 3 cut(s) 105, 201, 1277
Csp6I GTAC 3 cut(s) 301, 400, 1643
CviAII CATG 4 cut(s) 253, 1157, 1189, 1280
CviQI GTAC 3 cut(s) 301, 400, 1643
DdeI CTNAG 5 cut(s) 59, 473, 681, 867, 1238
DpnI GATC 7 cut(s) 459, 798, 969, 1164, 1608, 1932, 1995
DpnII GATC 7 cut(s) 457, 796, 967, 1162, 1606, 1930, 1993
DraI TTTAAA 1 cut(s) 1651
DraIII CACNNNGTG 1 cut(s) 79
DrdI GACNNNNNNGTC 1 cut(s) 1274
DriI GACNNNNNGTC 1 cut(s) 1945
DseDI GACNNNNNNGTC 1 cut(s) 1274
EaeI YGGCCR 2 cut(s) 517, 1698
Eam1104I CTCTTC 1 cut(s) 1893
Eam1105I GACNNNNNGTC 1 cut(s) 1945
EarI CTCTTC 1 cut(s) 1893
EciI GGCGGA 1 cut(s) 1216
Ecl136II GAGCTC 3 cut(s) 63, 71, 1820
Eco130I CCWWGG 4 cut(s) 105, 252, 411, 1156
Eco24I GRGCYC 3 cut(s) 65, 73, 1822
Eco47I GGWCC 2 cut(s) 1347, 1792
Eco53kI GAGCTC 3 cut(s) 63, 71, 1820
Eco72I CACGTG 1 cut(s) 1016
EcoICRI GAGCTC 3 cut(s) 63, 71, 1820
EcoNI CCTNNNNNAGG 1 cut(s) 1637
EcoO109I RGGNCCY 1 cut(s) 497
EcoRI GAATTC 1 cut(s) 1845
EcoRII CCWGG 3 cut(s) 519, 1171, 1342
EcoT14I CCWWGG 4 cut(s) 105, 252, 411, 1156
EcoT38I GRGCYC 3 cut(s) 65, 73, 1822
ErhI CCWWGG 4 cut(s) 105, 252, 411, 1156
FaeI CATG 4 cut(s) 256, 1160, 1192, 1283
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 2 cut(s) 452, 1360
FatI CATG 4 cut(s) 252, 1156, 1188, 1279
FauI CCCGC 1 cut(s) 211
FauNDI CATATG 2 cut(s) 1210, 1582
FblI GTMKAC 4 cut(s) 407, 714, 1461, 1749
FokI GGATG 4 cut(s) 1155, 1460, 1490, 1922
FriOI GRGCYC 3 cut(s) 65, 73, 1822
FspBI CTAG 4 cut(s) 243, 989, 1022, 1679
GlaI GCGC 3 cut(s) 99, 247, 1595
GsaI CCCAGC 2 cut(s) 1619, 1983
HaeII RGCGCY 2 cut(s) 249, 1597
HaeIII GGCC 4 cut(s) 499, 519, 769, 1700
HapII CCGG 4 cut(s) 113, 516, 1440, 1484
HgaI GACGC 3 cut(s) 105, 201, 1277
HhaI GCGC 3 cut(s) 100, 248, 1596
Hin1I GRCGYC 2 cut(s) 116, 212
Hin1II CATG 4 cut(s) 256, 1160, 1192, 1283
Hin6I GCGC 3 cut(s) 98, 246, 1594
HinP1I GCGC 3 cut(s) 98, 246, 1594
HindIII AAGCTT 2 cut(s) 896, 1856
HinfI GANTC 8 cut(s) 34, 165, 734, 1113, 1519, 1880, 1920, 2015
HpaII CCGG 4 cut(s) 113, 516, 1440, 1484
HphI GGTGA 3 cut(s) 902, 977, 1312
Hpy188III TCNNGA 9 cut(s) 66, 308, 339, 584, 794, 1523, 1565, 1600, 1764
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 7 cut(s) 218, 429, 510, 610, 628, 956, 1835
HpyCH4IV ACGT 5 cut(s) 591, 725, 730, 1015, 1710
HpyCH4V TGCA 9 cut(s) 576, 847, 861, 1053, 1208, 1546, 1736, 1774, 1955
HpyF10VI GCNNNNNNNGC 9 cut(s) 21, 139, 215, 282, 1050, 1198, 1243, 1379, 1742
HpyF3I CTNAG 5 cut(s) 59, 473, 681, 867, 1238
HpySE526I ACGT 5 cut(s) 591, 725, 730, 1015, 1710
Hsp92I GRCGYC 2 cut(s) 116, 212
Hsp92II CATG 4 cut(s) 256, 1160, 1192, 1283
HspAI GCGC 3 cut(s) 98, 246, 1594
KroI GCCGGC 1 cut(s) 515
KroNI GCCGGC 1 cut(s) 517
Kzo9I GATC 7 cut(s) 457, 796, 967, 1162, 1606, 1930, 1993
LguI GCTCTTC 1 cut(s) 1893
LmnI GCTCC 8 cut(s) 115, 138, 201, 256, 344, 907, 1345, 1817
Lsp1109I GCAGC 6 cut(s) 271, 481, 682, 704, 1558, 1737
LweI GCATC 4 cut(s) 64, 846, 1539, 1989
MaeI CTAG 4 cut(s) 243, 989, 1022, 1679
MaeII ACGT 5 cut(s) 591, 725, 730, 1015, 1710
MaeIII GTNAC 6 cut(s) 25, 357, 726, 731, 1435, 1711
MalI GATC 7 cut(s) 459, 798, 969, 1164, 1608, 1932, 1995
MboI GATC 7 cut(s) 457, 796, 967, 1162, 1606, 1930, 1993
MfeI CAATTG 2 cut(s) 230, 348
MflI RGATCY 4 cut(s) 796, 1162, 1606, 1993
MhlI GDGCHC 5 cut(s) 65, 73, 466, 1055, 1822
MlsI TGGCCA 1 cut(s) 1700
MluNI TGGCCA 1 cut(s) 1700
MlyI GAGTC 5 cut(s) 28, 728, 1122, 1513, 1914
MmeI TCCRAC 2 cut(s) 1090, 1381
Mox20I TGGCCA 1 cut(s) 1700
MroNI GCCGGC 1 cut(s) 515
MroXI GAANNNNTTC 3 cut(s) 945, 1097, 1845
MscI TGGCCA 1 cut(s) 1700
MseI TTAA 8 cut(s) 510, 756, 830, 1071, 1103, 1422, 1449, 1650
Msp20I TGGCCA 1 cut(s) 1700
MspA1I CMGCKG 1 cut(s) 695
MspI CCGG 4 cut(s) 113, 516, 1440, 1484
MspR9I CCNGG 5 cut(s) 521, 1173, 1344, 1441, 1484
MunI CAATTG 2 cut(s) 230, 348
Mva1269I GAATGC 4 cut(s) 144, 576, 1044, 1736
MvaI CCWGG 3 cut(s) 521, 1173, 1344
MvnI CGCG 4 cut(s) 32, 67, 196, 276
MwoI GCNNNNNNNGC 9 cut(s) 21, 139, 215, 282, 1050, 1198, 1243, 1379, 1742
NaeI GCCGGC 1 cut(s) 517
NciI CCSGG 2 cut(s) 1441, 1484
NcoI CCATGG 2 cut(s) 252, 1156
NdeI CATATG 2 cut(s) 1210, 1582
NdeII GATC 7 cut(s) 457, 796, 967, 1162, 1606, 1930, 1993
NgoMIV GCCGGC 1 cut(s) 515
NlaIII CATG 4 cut(s) 256, 1160, 1192, 1283
NlaIV GGNNCC 6 cut(s) 111, 134, 498, 1164, 1341, 1348
NmeAIII GCCGAG 2 cut(s) 108, 445
NmuCI GTSAC 2 cut(s) 731, 1711
NruI TCGCGA 1 cut(s) 67
NspV TTCGAA 2 cut(s) 270, 1429
PciSI GCTCTTC 1 cut(s) 1893
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 362
PctI GAATGC 4 cut(s) 144, 576, 1044, 1736
PdiI GCCGGC 1 cut(s) 517
PdmI GAANNNNTTC 3 cut(s) 945, 1097, 1845
PfeI GAWTC 3 cut(s) 165, 1880, 2015
PflMI CCANNNNNTGG 1 cut(s) 1172
PfoI TCCNGGA 2 cut(s) 1171, 1482
PleI GAGTC 5 cut(s) 28, 728, 1121, 1513, 1914
PmaCI CACGTG 1 cut(s) 1016
PmlI CACGTG 1 cut(s) 1016
PpsI GAGTC 5 cut(s) 28, 728, 1121, 1513, 1914
Ppu21I YACGTR 2 cut(s) 731, 1016
PshAI GACNNNNGTC 1 cut(s) 1352
Psp124BI GAGCTC 3 cut(s) 65, 73, 1822
Psp6I CCWGG 3 cut(s) 519, 1171, 1342
PspCI CACGTG 1 cut(s) 1016
PspFI CCCAGC 2 cut(s) 1615, 1979
PspGI CCWGG 3 cut(s) 519, 1171, 1342
PspN4I GGNNCC 6 cut(s) 111, 134, 498, 1164, 1341, 1348
PspPI GGNCC 3 cut(s) 497, 1347, 1792
PsrI GAACNNNNNNTAC 2 cut(s) 1620, 1652
PstI CTGCAG 1 cut(s) 849
PstNI CAGNNNCTG 3 cut(s) 58, 314, 853
PsuI RGATCY 4 cut(s) 796, 1162, 1606, 1993
PvuII CAGCTG 1 cut(s) 695
RruI TCGCGA 1 cut(s) 67
RsaI GTAC 3 cut(s) 302, 401, 1644
RsaNI GTAC 3 cut(s) 301, 400, 1643
SacI GAGCTC 3 cut(s) 65, 73, 1822
SapI GCTCTTC 1 cut(s) 1893
SaqAI TTAA 8 cut(s) 510, 756, 830, 1071, 1103, 1422, 1449, 1650
Sau3AI GATC 7 cut(s) 457, 796, 967, 1162, 1606, 1930, 1993
Sau96I GGNCC 3 cut(s) 497, 1347, 1792
SchI GAGTC 5 cut(s) 28, 728, 1122, 1513, 1914
ScrFI CCNGG 5 cut(s) 521, 1173, 1344, 1441, 1484
SduI GDGCHC 5 cut(s) 65, 73, 466, 1055, 1822
SfaNI GCATC 4 cut(s) 64, 846, 1539, 1989
SfcI CTRYAG 2 cut(s) 845, 1746
SfuI TTCGAA 2 cut(s) 270, 1429
SinI GGWCC 2 cut(s) 1347, 1792
SmlI CTYRAG 2 cut(s) 1521, 1565
SmoI CTYRAG 2 cut(s) 1521, 1565
SspI AATATT 1 cut(s) 937
SspMI CTAG 4 cut(s) 243, 989, 1022, 1679
SstI GAGCTC 3 cut(s) 65, 73, 1822
StyD4I CCNGG 5 cut(s) 519, 1171, 1342, 1439, 1482
StyI CCWWGG 4 cut(s) 105, 252, 411, 1156
TaiI ACGT 5 cut(s) 594, 728, 733, 1018, 1713
TaqI TCGA 7 cut(s) 189, 264, 270, 365, 621, 654, 1429
TaqII GACCGA 1 cut(s) 1364
TatI WGTACW 2 cut(s) 300, 399
TauI GCSGC 8 cut(s) 185, 212, 251, 279, 772, 1124, 1132, 1678
TfiI GAWTC 3 cut(s) 165, 1880, 2015
Tru1I TTAA 8 cut(s) 510, 756, 830, 1071, 1103, 1422, 1449, 1650
Tru9I TTAA 8 cut(s) 510, 756, 830, 1071, 1103, 1422, 1449, 1650
TscAI CASTG 3 cut(s) 82, 1123, 1804
TseFI GTSAC 2 cut(s) 731, 1711
TseI GCWGC 6 cut(s) 259, 469, 692, 695, 1546, 1725
Tsp45I GTSAC 2 cut(s) 731, 1711
TspDTI ATGAA 7 cut(s) 17, 496, 578, 813, 897, 1819, 1854
TspGWI ACGGA 5 cut(s) 27, 1466, 1496, 1613, 1889
TspRI CASTG 3 cut(s) 82, 1123, 1804
Van91I CCANNNNNTGG 1 cut(s) 1172
VneI GTGCAC 1 cut(s) 1051
VpaK11BI GGWCC 2 cut(s) 1347, 1792
XagI CCTNNNNNAGG 1 cut(s) 1637
XapI RAATTY 7 cut(s) 4, 672, 884, 981, 1142, 1257, 1845
XmiI GTMKAC 4 cut(s) 407, 714, 1461, 1749
XmnI GAANNNNTTC 3 cut(s) 945, 1097, 1845
XspI CTAG 4 cut(s) 243, 989, 1022, 1679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.