pycom15g09950

aarF domain-containing protein kinase At4g31390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001871
Physical Location & Seq
Reverse (-)
6479260 .. 6491938
12679 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g09950.23

Sequence Viewer

Length: 4485 bp
ATGGAAGAAGAGAGGATTTGCAGATTCATCCCATTCCCATTTCACTCTCTGAATCTCTCAGCCTCCACTCCCAGAGCAGAATCCATGAATTTTCTCTGCGTAAATTGCGTAACCGCAGCTCCGTTCCCTGCCAATCAGCATCTGAGCTCGCGAGCTTACACTGTGCCGAGCCGTAGAAATGCGCCGTCCAAGGCTCCGGCGTCGTTTGATTACAGGCTCCGAATGCGAGGAGGCAAGAAACCGTTGCGATTCCGAGTTTCTAATGCCGCAATCGAAACGCGGAGGAGCTTGGCGGCGTCTGCGGGCGAAGGGCAATTGACCAAACCTAGCGCTGCCATGGAGCAGCTCGACTTCGAACGCGGCGTTTGCGTGCCGTTTCGCAAGTACAGTCCAGAAACTGTGAGAAATACGGTGTTGAAATCCAGAGGAGCAATTGTGTCGTTACTTTCGAGGGGTGTGGAGATTGTGTGGAGTTTGGGATTGTACTGGTCTACCTTGGCGTATGACTGCTTCGTAGGAAGGGACGAAGAAGTTGTTTCAGATCGTGCTCGGCAGCTTAGAAACCTCCTATGTGATTTGGGGCCTTCTTTCATTAAAGCCGGCCAGGTTCTTGCAAACAGACCTGATATTATCAGAGAAGATTATATGAACGAACTGTGCATTCTTCAAGATGACGTTCCTCCCTTCCCCAATCAGGTTGCCTTCAACATAATAGAAGAAGAAATGGGGCGACCCCTTGAAGCTGTTTTCAGTAAAATTTCTTCTCAGACGATAGCAGCTGCCAGTTTAGGTCAAGTATACCGAGCTACGTTACGTGACTCTGGCAAGGAAGTTGCTATTAAGGTTCAAAGGCCGCAGATAGAACCTATAATATATCGGGATCTTTTTCTGTTCCGAACTCTTGCTTCATTCTTAAATGGCATCAGTCTGCAGAAACTGGGGTGCAATGCTGAGCTGATTGTTGATGAATTTGGTGAGAAGCTTTTGGAGGAGCTTGATTACACTTTGGAAGCCCGTAATATTGAAGATTTTCTTCTGAACTTTGAAGGTGATCCTACTGTCAAAATTCCTCTAGTTTACAAAGAGCTTTGTAGTTCACGTGTTCTAGTGATGGAGTGGATTGATGGCATTCGGTGCACTAACCCACGGGCTGTTAAAGAAGCTGGTATTGATGTGAATGGGTTCTTAACGGTTGGAGTCAGTGCTGCATTGCGGCAATTACTGGAATTTGGGTTGTTCCATGGGGATCCACATCCTGGAAATGTTTTTGCCATGCGTGATGGGCGTATTGCATATGTGGACTTTGGGAATGTTGCTGTGCTTAGTCAGCAAAATAAACAAATTTTGATTGACGCTGTTGTCCATGCTGTAAACGAGGACTATGTTGAGATGGCAAATGATTTCACCAGACTTGGCTTCTTGGCTCCTGGGACCGATGTCTCTCCTATTGTTCCAGCTTTAGAATCGATATGGCAGAACTCTGTTGGAAAAGGACTGTCTGACTTTAATTTTCGAAGCGTTACCGGGAAGTTTAACCAATTAGTCTACAACTATCCCATCCGTATCCCGGAGAGGTTTTCCCTTGTCATCCGTTCTTTATTGACTCAAGAGGGCATCTGTTTTACTTTGCAGCCAGACTTCAAGTTTCTTGAGGTTGCCTATCCATATGTGGCAAAGCGCCTCCTGACAGATCCAAACCCAGCTCTCCGTGAACGCCTCATACAGGTACTTTTTAAAGATGGTGTTTTCCAATGGAAGCGGCTAGAAAACCTTATTGTTCTGGCCAAAGAAAACGTAACCAAGATGAACAGCAATCCTGCATTTCAAGCTGTAGACACGCAACGGTCAAGAGAATTGCAAGTTAGTAGGAAACTGGACCTCACTGACACCATCAAAGATGGAGCTCGCCTTTTCATTTTTGATGAAGGAATTCGTAGAAAGCTTATTCTTGCTTTGACGGAAGATTCAAGGCTTCACATTGAAGAGATAGACATACCGTCAGTCGCAATGGAAGTAGCACGAGATTTCCCAACCGTTATACGAGATCTGATGCTTTCGTGGAGTGATTCGGAAACGAACAAGAAGAAGAAGAAGAAAAGGAAAAAGTACCATCTTCTCCCTTTGCTCCTCACTACTCCTGCTGCACCAGCTTCATTCATGTGGCAGAGAAATAGAGCAATTCATTGCGCAGCTGGAATTCAAACTCTTACGATAGGCTGCGGCGGTGTATGCACTGGGACCATGCCTCATAAAGTAAACTACGGAATTGATTATGATGATGAGTACGATGCTTATGAAGATTATGATTATGATTATGATGATGATGATGATGAGTCTGGAATAGAAAAGAATGTAACAGGCAAAGCAGTTGAGTCAAAGCGGGAAACTGAAAGGCCTAAGGTCTGGAGTTGTTCCATTTGCACGTATGATAATGAAGAGAATATGTCTGCATGTGACATTTGTGGGGTTCTTCGCAACCCTTTGTCTAAAAGCAATAGAGATAATAAAACAGTTGAAGGCAGATGCAAAGATTCTGGAGTATCTATAATGGCCAAGTCTATTTTTGCATCATTGCCGCACCAGAAGCCAAAACAGGCTGTACTATTTCATCAGCAGAAAGATGATTCTATGATAGAAGAGGGCAATAACTTCCACAGGCTTGGGGATTTCAAAGGACCTATTCATGAATTCCATAACACTTTTAATGTTCATAACAAACACCGTATTGATATAGCTCCTTTCAAGTTTGATGTTCCATCCCCAGATGACGTGGTTTCTAATGGATTGTGGTCCTCCAAAACAGGTTCAAAAGCTGGTGTTGTTGACTTGAAATCTTCAAAAGCTTCCTCAAATGTTGCGAAGAAGAGTAGTGTAGTTAGCATACAATCAAGTGCTGAATGGTCAGGTAGCTCATCTACAATGGCACAAAAAAGGGAAATTACTATACAAGAAAATGGTAAATTTTCAGAGAGTTCATCTGCTTCGCAGCCAACAAGCAGACGTGACAGTGGGGTTGAGAGCACTTCTGAAATTAGGGGCAAGTCACAGAGTGTTACCAGTGGCTTGAAAAACATGACTTTGGATGCTCGATCTGGATATTCTAATAGTGTTAGTGTTGGAGAATCTCGTTCACAAGCGGACTATAAACCCGAGAAGTGGATGCTTCCTGACCAAGCTGTAGATACACTGACTCAACTGAATCTTGCAATAGTTGGTCATGTTGATTCTGGAAAATCAACACTCTCAGGTAGGTTGCTTCACCTTTTGGGAAGGATATCTCAGAAAGAAATGCACAAATATGAGAAAGAGGCAAACTTGCAGGGAAAGGGGTCCTTCGCATATGCTTGGGCATTGGATGAGAGTACCGAAGAGAGGGAAAGAGGAATAACTATGACAGTGGCTGTTGCATACTTTGATTCCAGAAAATATCATGTTGTTGTGCTTGACTCACCTGGCCATAAAGATTTTGTTCCTAACATGATATCTGGGGCAACACAAGCTGATGCTGCGATACTTCTCATAGATGCGTCTGTTGGTGCATTCGAAGCTGGTATGGATGTTGGCAAGGGACAGACAAGGGAGCATGCACAACTTATTAGAAGTTTTGGTGTTGATCAAATTATAGTTGCTATTAACAAGATGGATATTGTTGAGTATTCCAAGGATCGATTTGATTTGATCAGACAGACACTTGGAACATTTCTCCGTTCCTGTGGTTTCAGAGATTCTTGGGTGTCTTGGATTCCATTGAGTGCCATGGAAAATCAGAATTTGGTGGCAGCTCCTTCTGATGTCCGTTTATTGTCTTGGTACCGTGGACCTTACCTGTTGGATGCAATTGATTCCCTCAAACCTCCTACAAGAGAGTTTTCAAAACCATTGCTGATGCCTATATGTGATGTCATCAAATCTTCTTCACAAGGACAGGTGTCTGCTTGTGGTAAATTGGAAGCAGGAGCTCTTCGAAGTGGATTCAAGGTTCTGGTTATGCCTTCGGGAGAATCAGGGACTGTACGCTCGTTAGAACGTGACTCTCAGGCTTGTGCAATTGCAAGGGCTGGAGACAATGTGGCTGTTACTCTGCAAGGCATTGATGGAGGTCGTGTGATGGCAGGAGGTGTGCTCTGTCACCCAAGTTTTCCAGTTGCAGTTGCAAAACATTTGGAATTGAAGGTGCTTGTTTTGGATGTTACAACCCCAATTTTAATTGGCTCTCAGTTGGAATTCCACATACACCACGCGAAGGAGGCTGCGAGAGTGGTAAAGATTTCATCATTGCTTGATCCAAAGACTGGAAAAGTGACGAGAAAGGCTCCTCGCTGTCTTACTGCAAAGCAGAGCGCGGTCATAGAGGTAATTCTTCATGCACCGGTTTGTGTTGAAGAGTTCTCCAACTCCAGAGCCCTTGGGAGGGCCTTTTTGAGGGCGTTAGGAAGCACTATTGCTGTCGGTGTTGTAACCCGGATTATAGAGGAGCAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006464 GO:0006468 GO:0006725 GO:0006778 GO:0006787 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0006995 GO:0006996 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009267 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009637 GO:0009639 GO:0009657 GO:0009658 GO:0009668 GO:0009767 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0009987 GO:0009991 GO:0010027 GO:0010033 GO:0010035 GO:0010109 GO:0010114 GO:0010287 GO:0010380 GO:0015979 GO:0015994 GO:0015996 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019216 GO:0019222 GO:0019439 GO:0019538 GO:0019684 GO:0019747 GO:0022900 GO:0031279 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031537 GO:0031540 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033015 GO:0033554 GO:0034641 GO:0036211 GO:0042221 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043562 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044270 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046149 GO:0046483 GO:0046700 GO:0046889 GO:0046890 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051186 GO:0051187 GO:0051193 GO:0051194 GO:0051716 GO:0055114 GO:0061024 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080177 GO:0080183 GO:0090056 GO:0140096 GO:1901360 GO:1901361 GO:1901401 GO:1901403 GO:1901463 GO:1901465 GO:1901562 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902171 GO:1902326 GO:1904143
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

1495

Amino Acids

165.75

Weight (kDa)

7.5

Isoelectric Point (pI)

44.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1358
Acc16I TGCGCA 1 cut(s) 2188
Acc65I GGTACC 1 cut(s) 3810
AccB1I GGYRCC 1 cut(s) 3810
AccB7I CCANNNNNTGG 2 cut(s) 1256, 4292
AccI GTMKAC 4 cut(s) 491, 798, 1545, 1833
AccII CGCG 5 cut(s) 151, 280, 360, 4241, 4343
AclWI GGATC 7 cut(s) 888, 1046, 1241, 1254, 1685, 3672, 4277
AcoI YGGCCR 4 cut(s) 601, 1782, 2550, 3454
AcvI CACGTG 1 cut(s) 1100
AcyI GRCGYC 2 cut(s) 200, 296
AdeI CACNNNGTG 1 cut(s) 3050
AfaI GTAC 9 cut(s) 386, 485, 1728, 2108, 2285, 2601, 3364, 3812, 4014
AfeI AGCGCT 1 cut(s) 331
AflIII ACRYGT 1 cut(s) 1099
AgeI ACCGGT 1 cut(s) 4369
AhdI GACNNNNNGTC 1 cut(s) 1996
AjiI CACGTC 2 cut(s) 2770, 3002
AjnI CCWGG 4 cut(s) 603, 1255, 1426, 3451
AloI GAACNNNNNNTCC 2 cut(s) 3963, 3995
Alw21I GWGCWC 7 cut(s) 149, 550, 1139, 1906, 3023, 3961, 4125
Alw26I GTCTC 2 cut(s) 1444, 4056
Alw44I GTGCAC 1 cut(s) 1135
AlwI GGATC 7 cut(s) 888, 1046, 1241, 1254, 1685, 3672, 4277
AlwNI CAGNNNCTG 5 cut(s) 142, 398, 937, 3401, 4010
Ama87I CYCGRG 1 cut(s) 3149
Aor51HI AGCGCT 1 cut(s) 331
AoxI GGCC 8 cut(s) 581, 601, 851, 1782, 2393, 2550, 3454, 4413
ApaLI GTGCAC 1 cut(s) 1135
AsiGI ACCGGT 1 cut(s) 4369
Asp700I GAANNNNTTC 3 cut(s) 1029, 1181, 1929
Asp718I GGTACC 1 cut(s) 3810
AspLEI GCGC 5 cut(s) 184, 332, 1680, 2189, 4343
AspS9I GGNCC 9 cut(s) 581, 1431, 1876, 2238, 2675, 2790, 3330, 3818, 4413
AsuC2I CCSGG 3 cut(s) 1525, 1568, 4462
AsuHPI GGTGA 6 cut(s) 986, 1061, 1396, 3251, 3441, 4121
AsuII TTCGAA 4 cut(s) 354, 1513, 3543, 3964
AvaI CYCGRG 1 cut(s) 3149
AvaII GGWCC 7 cut(s) 1431, 1876, 2238, 2675, 2790, 3330, 3818
AxyI CCTNAGG 1 cut(s) 2397
BaeGI GKGCMC 1 cut(s) 1139
BalI TGGCCA 3 cut(s) 1784, 2552, 3456
BamHI GGATCC 1 cut(s) 1246
BanI GGYRCC 1 cut(s) 3810
BanII GRGCYC 4 cut(s) 149, 1906, 3961, 4405
BarI GAAGNNNNNNTAC 4 cut(s) 494, 526, 745, 777
BauI CACGAG 1 cut(s) 2019
BbrPI CACGTG 1 cut(s) 1100
Bbv12I GWGCWC 7 cut(s) 149, 550, 1139, 1906, 3023, 3961, 4125
BceAI ACGGC 3 cut(s) 156, 169, 358
BcgI CGANNNNNNTGC 2 cut(s) 420, 454
BciT130I CCWGG 4 cut(s) 605, 1257, 1428, 3453
BciVI GTATCC 1 cut(s) 1574
BclI TGATCA 2 cut(s) 3613, 3678
BcnI CCSGG 3 cut(s) 1525, 1568, 4462
BcoDI GTCTC 2 cut(s) 1444, 4056
BfaI CTAG 4 cut(s) 327, 1073, 1106, 1763
BfmI CTRYAG 3 cut(s) 929, 1830, 3177
BfoI RGCGCY 2 cut(s) 333, 1681
BfuI GTATCC 1 cut(s) 1574
BglII AGATCT 1 cut(s) 2044
BlpI GCTNAGC 1 cut(s) 951
Bme1390I CCNGG 7 cut(s) 605, 1257, 1428, 1525, 1568, 3453, 4462
Bme18I GGWCC 7 cut(s) 1431, 1876, 2238, 2675, 2790, 3330, 3818
BmeRI GACNNNNNGTC 1 cut(s) 1996
BmeT110I CYCGRG 1 cut(s) 3149
BmgBI CACGTC 2 cut(s) 2770, 3002
BmgT120I GGNCC 9 cut(s) 581, 1431, 1876, 2238, 2675, 2790, 3330, 3818, 4413
BmrFI CCNGG 7 cut(s) 605, 1257, 1428, 1525, 1568, 3453, 4462
BmrI ACTGGG 2 cut(s) 947, 2244
BmuI ACTGGG 2 cut(s) 947, 2244
BoxI GACNNNNGTC 2 cut(s) 1436, 3928
BplI GAGNNNNNCTC 4 cut(s) 4107, 4139, 4297, 4329
BpmI CTGGAG 4 cut(s) 2425, 2556, 4080, 4381
Bpu1102I GCTNAGC 1 cut(s) 951
Bpu14I TTCGAA 4 cut(s) 354, 1513, 3543, 3964
BpuEI CTTGAG 2 cut(s) 1590, 1670
BpuMI CCSGG 3 cut(s) 1525, 1568, 4462
Bsa29I ATCGAT 2 cut(s) 1466, 3667
BsaAI YACGTR 3 cut(s) 815, 1100, 2424
BsaBI GATNNNNATC 1 cut(s) 1251
BsaHI GRCGYC 2 cut(s) 200, 296
BsaWI WCCGGW 1 cut(s) 4369
Bse118I RCCGGY 2 cut(s) 599, 4369
Bse1I ACTGG 9 cut(s) 491, 783, 942, 1227, 1878, 2239, 3057, 4142, 4297
Bse21I CCTNAGG 1 cut(s) 2397
Bse3DI GCAATG 7 cut(s) 952, 1208, 2013, 2182, 2570, 3878, 4274
Bse8I GATNNNNATC 1 cut(s) 1251
BseBI CCWGG 4 cut(s) 605, 1257, 1428, 3453
BseCI ATCGAT 2 cut(s) 1466, 3667
BseJI GATNNNNATC 1 cut(s) 1251
BseMI GCAATG 7 cut(s) 952, 1208, 2013, 2182, 2570, 3878, 4274
BseMII CTCAG 8 cut(s) 72, 134, 779, 942, 3258, 3293, 4049, 4229
BseNI ACTGG 9 cut(s) 491, 783, 942, 1227, 1878, 2239, 3057, 4142, 4297
BseRI GAGGAG 6 cut(s) 243, 298, 441, 1004, 2117, 4305
BseSI GKGCMC 1 cut(s) 1139
BseYI CCCAGC 1 cut(s) 1699
BsgI GTGCAG 1 cut(s) 2127
Bsh1236I CGCG 5 cut(s) 151, 280, 360, 4241, 4343
BshFI GGCC 8 cut(s) 583, 603, 853, 1784, 2395, 2552, 3456, 4415
BshNI GGYRCC 1 cut(s) 3810
BshTI ACCGGT 1 cut(s) 4369
BshVI ATCGAT 2 cut(s) 1466, 3667
BsiHKAI GWGCWC 7 cut(s) 149, 550, 1139, 1906, 3023, 3961, 4125
BsiHKCI CYCGRG 1 cut(s) 3149
BsiSI CCGG 6 cut(s) 197, 600, 1524, 1568, 4370, 4462
BslFI GGGAC 5 cut(s) 536, 1444, 2251, 3582, 4021
BsmAI GTCTC 2 cut(s) 1444, 4056
BsmFI GGGAC 5 cut(s) 536, 1444, 2251, 3582, 4021
BsmI GAATGC 4 cut(s) 228, 660, 1128, 3539
BsnI GGCC 8 cut(s) 583, 603, 853, 1784, 2395, 2552, 3456, 4415
BsoBI CYCGRG 1 cut(s) 3149
Bsp119I TTCGAA 4 cut(s) 354, 1513, 3543, 3964
Bsp1286I GDGCHC 8 cut(s) 149, 550, 1139, 1906, 3023, 3961, 4125, 4405
Bsp1720I GCTNAGC 1 cut(s) 951
Bsp19I CCATGG 3 cut(s) 336, 1240, 3756
Bsp68I TCGCGA 1 cut(s) 151
BspANI GGCC 8 cut(s) 583, 603, 853, 1784, 2395, 2552, 3456, 4415
BspCNI CTCAG 8 cut(s) 71, 135, 778, 943, 3257, 3292, 4048, 4228
BspDI ATCGAT 2 cut(s) 1466, 3667
BspFNI CGCG 5 cut(s) 151, 280, 360, 4241, 4343
BspHI TCATGA 1 cut(s) 2683
BspMAI CTGCAG 1 cut(s) 933
BspPI GGATC 7 cut(s) 888, 1046, 1241, 1254, 1685, 3672, 4277
BspQI GCTCTTC 1 cut(s) 3966
BspT104I TTCGAA 4 cut(s) 354, 1513, 3543, 3964
BspT107I GGYRCC 1 cut(s) 3810
BsrDI GCAATG 7 cut(s) 952, 1208, 2013, 2182, 2570, 3878, 4274
BsrFI RCCGGY 2 cut(s) 599, 4369
BsrI ACTGG 9 cut(s) 491, 783, 942, 1227, 1878, 2239, 3057, 4142, 4297
BssAI RCCGGY 2 cut(s) 599, 4369
BssNAI GTATAC 1 cut(s) 799
BssNI GRCGYC 2 cut(s) 200, 296
BssSI CACGAG 1 cut(s) 2019
BssT1I CCWWGG 7 cut(s) 189, 336, 495, 1240, 3660, 3756, 4405
Bst1107I GTATAC 1 cut(s) 799
Bst2BI CACGAG 1 cut(s) 2019
Bst2UI CCWGG 4 cut(s) 605, 1257, 1428, 3453
Bst6I CTCTTC 8 cut(s) 3, 1977, 2430, 2631, 2858, 3363, 3966, 4377
BstACI GRCGYC 2 cut(s) 200, 296
BstAPI GCANNNNNTGC 1 cut(s) 1134
BstBAI YACGTR 3 cut(s) 815, 1100, 2424
BstBI TTCGAA 4 cut(s) 354, 1513, 3543, 3964
BstC8I GCNNGC 7 cut(s) 149, 153, 304, 371, 601, 1906, 3585
BstDSI CCRYGG 5 cut(s) 336, 1145, 1240, 3756, 3814
BstENI CCTNNNNNAGG 2 cut(s) 1721, 4420
BstFNI CGCG 5 cut(s) 151, 280, 360, 4241, 4343
BstH2I RGCGCY 2 cut(s) 333, 1681
BstHHI GCGC 5 cut(s) 184, 332, 1680, 2189, 4343
BstMAI GTCTC 2 cut(s) 1444, 4056
BstNI CCWGG 4 cut(s) 605, 1257, 1428, 3453
BstNSI RCATGY 2 cut(s) 2454, 3587
BstPAI GACNNNNGTC 2 cut(s) 1436, 3928
BstSCI CCNGG 7 cut(s) 603, 1255, 1426, 1523, 1566, 3451, 4460
BstSFI CTRYAG 3 cut(s) 929, 1830, 3177
BstSLI GKGCMC 1 cut(s) 1139
BstUI CGCG 5 cut(s) 151, 280, 360, 4241, 4343
BstX2I RGATCY 4 cut(s) 880, 1246, 1690, 2044
BstXI CCANNNNNNTGG 1 cut(s) 2660
BstYI RGATCY 4 cut(s) 880, 1246, 1690, 2044
BstZ17I GTATAC 1 cut(s) 799
Bsu15I ATCGAT 2 cut(s) 1466, 3667
Bsu36I CCTNAGG 1 cut(s) 2397
BsuI GTATCC 1 cut(s) 1574
BsuRI GGCC 8 cut(s) 583, 603, 853, 1784, 2395, 2552, 3456, 4415
BsuTUI ATCGAT 2 cut(s) 1466, 3667
BtgI CCRYGG 5 cut(s) 336, 1145, 1240, 3756, 3814
BtrI CACGTC 2 cut(s) 2770, 3002
BtsIMutI CAGTG 8 cut(s) 159, 1207, 1881, 2232, 3013, 3064, 3185, 3402
BtuMI TCGCGA 1 cut(s) 151
Cac8I GCNNGC 7 cut(s) 149, 153, 304, 371, 601, 1906, 3585
CaiI CAGNNNCTG 5 cut(s) 142, 398, 937, 3401, 4010
CciI TCATGA 1 cut(s) 2683
CfoI GCGC 5 cut(s) 184, 332, 1680, 2189, 4343
Cfr10I RCCGGY 2 cut(s) 599, 4369
Cfr13I GGNCC 9 cut(s) 581, 1431, 1876, 2238, 2675, 2790, 3330, 3818, 4413
ClaI ATCGAT 2 cut(s) 1466, 3667
CseI GACGC 4 cut(s) 189, 285, 1361, 3516
Csp6I GTAC 9 cut(s) 385, 484, 1727, 2107, 2284, 2600, 3363, 3811, 4013
CspAI ACCGGT 1 cut(s) 4369
CviQI GTAC 9 cut(s) 385, 484, 1727, 2107, 2284, 2600, 3363, 3811, 4013
DraI TTTAAA 1 cut(s) 1735
DraIII CACNNNGTG 1 cut(s) 3050
DrdI GACNNNNNNGTC 1 cut(s) 1358
DriI GACNNNNNGTC 1 cut(s) 1996
DseDI GACNNNNNNGTC 1 cut(s) 1358
EaeI YGGCCR 4 cut(s) 601, 1782, 2550, 3454
Eam1104I CTCTTC 8 cut(s) 3, 1977, 2430, 2631, 2858, 3363, 3966, 4377
Eam1105I GACNNNNNGTC 1 cut(s) 1996
EarI CTCTTC 8 cut(s) 3, 1977, 2430, 2631, 2858, 3363, 3966, 4377
Ecl136II GAGCTC 3 cut(s) 147, 1904, 3959
Eco130I CCWWGG 7 cut(s) 189, 336, 495, 1240, 3660, 3756, 4405
Eco147I AGGCCT 1 cut(s) 2395
Eco24I GRGCYC 4 cut(s) 149, 1906, 3961, 4405
Eco32I GATATC 2 cut(s) 3276, 3483
Eco47I GGWCC 7 cut(s) 1431, 1876, 2238, 2675, 2790, 3330, 3818
Eco47III AGCGCT 1 cut(s) 331
Eco53kI GAGCTC 3 cut(s) 147, 1904, 3959
Eco72I CACGTG 1 cut(s) 1100
Eco81I CCTNAGG 1 cut(s) 2397
Eco88I CYCGRG 1 cut(s) 3149
EcoICRI GAGCTC 3 cut(s) 147, 1904, 3959
EcoNI CCTNNNNNAGG 2 cut(s) 1721, 4420
EcoO109I RGGNCCY 4 cut(s) 581, 2675, 3330, 4413
EcoRI GAATTC 4 cut(s) 1929, 2196, 2687, 4223
EcoRII CCWGG 4 cut(s) 603, 1255, 1426, 3451
EcoRV GATATC 2 cut(s) 3276, 3483
EcoT14I CCWWGG 7 cut(s) 189, 336, 495, 1240, 3660, 3756, 4405
EcoT38I GRGCYC 4 cut(s) 149, 1906, 3961, 4405
ErhI CCWWGG 7 cut(s) 189, 336, 495, 1240, 3660, 3756, 4405
FalI AAGNNNNNCTT 4 cut(s) 1017, 1049, 3317, 3349
FaqI GGGAC 5 cut(s) 536, 1444, 2251, 3582, 4021
FauI CCCGC 2 cut(s) 295, 2373
FauNDI CATATG 3 cut(s) 1294, 1666, 3340
FbaI TGATCA 2 cut(s) 3613, 3678
FblI GTMKAC 4 cut(s) 491, 798, 1545, 1833
FriOI GRGCYC 4 cut(s) 149, 1906, 3961, 4405
FspBI CTAG 4 cut(s) 327, 1073, 1106, 1763
FspI TGCGCA 1 cut(s) 2188
GlaI GCGC 5 cut(s) 183, 331, 1679, 2188, 4342
GsaI CCCAGC 1 cut(s) 1703
GsuI CTGGAG 4 cut(s) 2425, 2556, 4080, 4381
HaeII RGCGCY 2 cut(s) 333, 1681
HaeIII GGCC 8 cut(s) 583, 603, 853, 1784, 2395, 2552, 3456, 4415
HapII CCGG 6 cut(s) 197, 600, 1524, 1568, 4370, 4462
HgaI GACGC 4 cut(s) 189, 285, 1361, 3516
HhaI GCGC 5 cut(s) 184, 332, 1680, 2189, 4343
Hin1I GRCGYC 2 cut(s) 200, 296
Hin6I GCGC 5 cut(s) 182, 330, 1678, 2187, 4341
HinP1I GCGC 5 cut(s) 182, 330, 1678, 2187, 4341
HincII GTYRAC 1 cut(s) 2824
HindII GTYRAC 1 cut(s) 2824
HindIII AAGCTT 3 cut(s) 980, 1940, 2841
HpaII CCGG 6 cut(s) 197, 600, 1524, 1568, 4370, 4462
HphI GGTGA 6 cut(s) 986, 1061, 1396, 3251, 3441, 4121
Hpy99I CGWCG 1 cut(s) 205
HpyCH4IV ACGT 9 cut(s) 675, 809, 814, 1099, 1794, 2423, 2769, 3001, 4027
HpySE526I ACGT 9 cut(s) 675, 809, 814, 1099, 1794, 2423, 2769, 3001, 4027
Hsp92I GRCGYC 2 cut(s) 200, 296
HspAI GCGC 5 cut(s) 182, 330, 1678, 2187, 4341
KpnI GGTACC 1 cut(s) 3814
KroI GCCGGC 1 cut(s) 599
KroNI GCCGGC 1 cut(s) 601
Ksp22I TGATCA 2 cut(s) 3613, 3678
LguI GCTCTTC 1 cut(s) 3966
MaeI CTAG 4 cut(s) 327, 1073, 1106, 1763
MaeII ACGT 9 cut(s) 675, 809, 814, 1099, 1794, 2423, 2769, 3001, 4027
MfeI CAATTG 4 cut(s) 314, 432, 3837, 4047
MflI RGATCY 4 cut(s) 880, 1246, 1690, 2044
MhlI GDGCHC 8 cut(s) 149, 550, 1139, 1906, 3023, 3961, 4125, 4405
MlsI TGGCCA 3 cut(s) 1784, 2552, 3456
MluNI TGGCCA 3 cut(s) 1784, 2552, 3456
MlyI GAGTC 8 cut(s) 812, 1206, 1597, 2342, 2381, 3184, 3440, 4025
MmeI TCCRAC 6 cut(s) 1174, 1465, 3097, 3810, 4200, 4416
Mox20I TGGCCA 3 cut(s) 1784, 2552, 3456
MroNI GCCGGC 1 cut(s) 599
MroXI GAANNNNTTC 3 cut(s) 1029, 1181, 1929
MscI TGGCCA 3 cut(s) 1784, 2552, 3456
MslI CAYNNNNRTG 2 cut(s) 2158, 3297
Msp20I TGGCCA 3 cut(s) 1784, 2552, 3456
MspA1I CMGCKG 2 cut(s) 779, 2192
MspI CCGG 6 cut(s) 197, 600, 1524, 1568, 4370, 4462
MspR9I CCNGG 7 cut(s) 605, 1257, 1428, 1525, 1568, 3453, 4462
MunI CAATTG 4 cut(s) 314, 432, 3837, 4047
Mva1269I GAATGC 4 cut(s) 228, 660, 1128, 3539
MvaI CCWGG 4 cut(s) 605, 1257, 1428, 3453
MvnI CGCG 5 cut(s) 151, 280, 360, 4241, 4343
NaeI GCCGGC 1 cut(s) 601
NciI CCSGG 3 cut(s) 1525, 1568, 4462
NcoI CCATGG 3 cut(s) 336, 1240, 3756
NdeI CATATG 3 cut(s) 1294, 1666, 3340
NgoMIV GCCGGC 1 cut(s) 599
NmeAIII GCCGAG 2 cut(s) 192, 529
NmuCI GTSAC 7 cut(s) 815, 2453, 3002, 3042, 4028, 4127, 4300
NruI TCGCGA 1 cut(s) 151
NsbI TGCGCA 1 cut(s) 2188
NspI RCATGY 2 cut(s) 2454, 3587
NspV TTCGAA 4 cut(s) 354, 1513, 3543, 3964
PaeI GCATGC 1 cut(s) 3587
PagI TCATGA 1 cut(s) 2683
PceI AGGCCT 1 cut(s) 2395
PciSI GCTCTTC 1 cut(s) 3966
PcsI WCGNNNNNNNCGW 2 cut(s) 360, 446
PctI GAATGC 4 cut(s) 228, 660, 1128, 3539
PdiI GCCGGC 1 cut(s) 601
PdmI GAANNNNTTC 3 cut(s) 1029, 1181, 1929
PflMI CCANNNNNTGG 2 cut(s) 1256, 4292
PfoI TCCNGGA 2 cut(s) 1255, 1566
PinAI ACCGGT 1 cut(s) 4369
PleI GAGTC 8 cut(s) 812, 1205, 1597, 2341, 2380, 3184, 3440, 4025
PmaCI CACGTG 1 cut(s) 1100
PmlI CACGTG 1 cut(s) 1100
PpsI GAGTC 8 cut(s) 812, 1205, 1597, 2341, 2380, 3184, 3440, 4025
Ppu21I YACGTR 3 cut(s) 815, 1100, 2424
PpuMI RGGWCCY 2 cut(s) 2675, 3330
PshAI GACNNNNGTC 2 cut(s) 1436, 3928
Psp124BI GAGCTC 3 cut(s) 149, 1906, 3961
Psp5II RGGWCCY 2 cut(s) 2675, 3330
Psp6I CCWGG 4 cut(s) 603, 1255, 1426, 3451
PspCI CACGTG 1 cut(s) 1100
PspFI CCCAGC 1 cut(s) 1699
PspGI CCWGG 4 cut(s) 603, 1255, 1426, 3451
PspPI GGNCC 9 cut(s) 581, 1431, 1876, 2238, 2675, 2790, 3330, 3818, 4413
PspPPI RGGWCCY 2 cut(s) 2675, 3330
PsrI GAACNNNNNNTAC 2 cut(s) 1704, 1736
PstI CTGCAG 1 cut(s) 933
PstNI CAGNNNCTG 5 cut(s) 142, 398, 937, 3401, 4010
PsuI RGATCY 4 cut(s) 880, 1246, 1690, 2044
PvuII CAGCTG 2 cut(s) 779, 2192
RruI TCGCGA 1 cut(s) 151
RsaI GTAC 9 cut(s) 386, 485, 1728, 2108, 2285, 2601, 3364, 3812, 4014
RsaNI GTAC 9 cut(s) 385, 484, 1727, 2107, 2284, 2600, 3363, 3811, 4013
RseI CAYNNNNRTG 2 cut(s) 2158, 3297
SacI GAGCTC 3 cut(s) 149, 1906, 3961
SapI GCTCTTC 1 cut(s) 3966
Sau96I GGNCC 9 cut(s) 581, 1431, 1876, 2238, 2675, 2790, 3330, 3818, 4413
SchI GAGTC 8 cut(s) 812, 1206, 1597, 2342, 2381, 3184, 3440, 4025
ScrFI CCNGG 7 cut(s) 605, 1257, 1428, 1525, 1568, 3453, 4462
SduI GDGCHC 8 cut(s) 149, 550, 1139, 1906, 3023, 3961, 4125, 4405
SfcI CTRYAG 3 cut(s) 929, 1830, 3177
SfuI TTCGAA 4 cut(s) 354, 1513, 3543, 3964
SinI GGWCC 7 cut(s) 1431, 1876, 2238, 2675, 2790, 3330, 3818
SmiMI CAYNNNNRTG 2 cut(s) 2158, 3297
SmlI CTYRAG 2 cut(s) 1605, 1649
SmoI CTYRAG 2 cut(s) 1605, 1649
SphI GCATGC 1 cut(s) 3587
SseBI AGGCCT 1 cut(s) 2395
SspI AATATT 1 cut(s) 1021
SspMI CTAG 4 cut(s) 327, 1073, 1106, 1763
SstI GAGCTC 3 cut(s) 149, 1906, 3961
StuI AGGCCT 1 cut(s) 2395
StyD4I CCNGG 7 cut(s) 603, 1255, 1426, 1523, 1566, 3451, 4460
StyI CCWWGG 7 cut(s) 189, 336, 495, 1240, 3660, 3756, 4405
TaiI ACGT 9 cut(s) 678, 812, 817, 1102, 1797, 2426, 2772, 3004, 4030
TaqII GACCGA 1 cut(s) 1448
TatI WGTACW 3 cut(s) 384, 483, 2599
TauI GCSGC 8 cut(s) 269, 296, 363, 856, 1216, 1762, 2223, 2578
TscAI CASTG 8 cut(s) 166, 1207, 1888, 2239, 3013, 3064, 3192, 3402
TseFI GTSAC 7 cut(s) 815, 2453, 3002, 3042, 4028, 4127, 4300
Tsp45I GTSAC 7 cut(s) 815, 2453, 3002, 3042, 4028, 4127, 4300
TspGWI ACGGA 8 cut(s) 111, 1550, 1580, 1697, 1973, 2277, 3695, 3785
TspRI CASTG 8 cut(s) 166, 1207, 1888, 2239, 3013, 3064, 3192, 3402
Van91I CCANNNNNTGG 2 cut(s) 1256, 4292
VneI GTGCAC 1 cut(s) 1135
VpaK11BI GGWCC 7 cut(s) 1431, 1876, 2238, 2675, 2790, 3330, 3818
XagI CCTNNNNNAGG 2 cut(s) 1721, 4420
XceI RCATGY 2 cut(s) 2454, 3587
XmiI GTMKAC 4 cut(s) 491, 798, 1545, 1833
XmnI GAANNNNTTC 3 cut(s) 1029, 1181, 1929
XspI CTAG 4 cut(s) 327, 1073, 1106, 1763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.