Rh6CG460400

aarF domain-containing protein kinase At4g31390

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0001871
Physical Location & Seq
Forward (+)
63447519 .. 63458591
11073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG460400.1

Sequence Viewer

Length: 3942 bp
ATGAATTCCGTTTCTGTTAACTGCACGCGCGCAGCTCCGTTTCCCGCCAAGCATCTGAGCTCCGCCGGAGCTCGTAGTGTTGCCGGTCAAATAAATGGACCGTCGGAAGGTTGGAGTTCGTTTGATTACAGGCCAAGAGTCCGGAGAAGCGGGAGGTTCCGAGTTTCGAATGCCGCGACCGGAACGCGGCGGAGCTTGGCGTCTCCGAGCGACGGCGAGATCGGTTTTGGGAAGCTGAGCAAGTCTAGCGCCGCCATGGAGCAGCTCGACTTCGAGCGCGGCGTTTGCGTGCCGTTTCGCAAGTATACTCCCGAGACTGTGAGGAATAAGGTGTTGGAATCCAATGGGGCAGTTGCATCGCTAATTTCGAGAGGTGTGGAGATAGTGTGGACTTTGGGCTTGTATTGGTCTGCCTTGATGTATGACTGCTTCGTCGGAAGGGATCAAGAAGTTGTTCCAAAGCGTGCTCGACAGCTTAGAAACCTCCTGTGTGATTTGGGGCCTTCTTTCATCAAAGCTGGCCAGGTTCTTGCAAACAGACCTGATATTATCAGAGAAGATTACATGAATGAACTTTGCATTCTCCAAGATGATGTTCCTCCGTTCCCCAATCAGGTTGCCTTTGACATAATAGAAGAGGAGTTGGGTCAACCCCTTGAAGCTGTATTCAGTAAGATTTCGCCACAGACAATAGCAGCTGCCAGTTTGGGTCAAGTTTACCGAGCTACTTTACGTGACACTGGCGAGGATGTAGCTATTAAGGTACAAAGGCCAGAGATAGAACCTATAATTTATAGGGATCTTTTTCTGTTCCGTAATCTTGCTTCATTCTTAAATGGAATCAGTCTACAGAAACTTGGGTGCAATGCTGAGCTCATTGTTGATGAATTCGGTGAGAAGCTTTTGGAGGAGCTTGATTACACTTTGGAAGCCCGCAATATTGAAGATTTTCTTGAGAACTTTAAAGATGATCCTACTGTCAAGATACCTGACGTTTACAAACAGCTTTCTGGTCCACGTGTTCTAGTAATGGAGTGGATCGATGGTGTTCGGTGCACTAACCCACAGGCAATTAGAGAAGCCGGTATTGATGTAAATGGGTTCTTAACTGTTGGAGTCAGTGCTGCGTTGCGCCAATTACTGGAATTTGGATTGTTCCATGGAGATCCACATCCTGGAAATGTTTTTGCTATGCGTGATGGACGTATTGGATATGTGGACTTTGGGAATGTTGCTGTGCTTAGTCAGCAAAACAAACAGATTTTGATCGATGCTGTCGTCCATGCTGTAAATGAGGACTATGTTGAGATGGCAAATGATTTCACCAGACTGGGCTTCTTGGCTCCTGGGACTGATGTCTCTCCTATTGTTCCAGCTTTAGAAGCAATCTGGCAGAATTCTCTTGGAAAAGGACTGTCTGACTTTAATTTTCGAAGTGTTACTGGGAAGTTTAATGAATTAGTTTACAACTATCCCATTCGTATTCCAGAGAGGTTTTCGCTTGTTATCAGATCTTTATTGACTCAAGAGGGTATCTGTTTTACTTTGCAGCCAGATTTCAAATTTCTTGAGGTTGCATATCCATATGTGGCAAAACGCCTCCTAACAGATCCAAACCCGGCTCTGCGTGAACGCCTCATACAAGTACTATTTAAGGATGGTGTTTTCCAATGGAAGCGGCTAGAAAATCTCATTGTTCTTGCAAAAGAAAATGTGTCCATGATGAGCAGCAATCCTGCATTGCAAGCGAAAGAAATGCAAGGGTTAAGAGAGTTGCAAGTTAGCAAGAAACTGGACCTCACTGACACCATCAAAGATGGAGCTCGCCTTTTCATTTTTGACGAAGGAATCCGTAGAAAGCTCATTCTTGCTTTGACCGAAGATTCAAAGCTTCACATAGAAGAGCTCGTGGATGTGTATAGACTGGTTGAAGATCAGATAGACATACCAACGGTTGCTGTGGAAGTAGCACGAGGCAAAGAACCTGAGTTAAAGCAGGAAACTGTAAGGCGTGATGTTTGGCGTTGTTCCATTTGTACATATGATAATGAAGAAGGTTTGACTGCGTGTGACATTTGTGGGGTTCTTCGTAAACCTTTGGGTAGAGTTGGAAGCAATATTGATAAGAAAACAGCTCCTTTCAAGTTTGATGTTCCATCCCCAGATGATGTGGTTTCCAATGGATTGCGATCCTCCAAAGTGGGTTCAAAAGCCAGTGTTAGTGACGTAGAGTCTTCGGGAGTTTCCTCGAAAGCTGCTAAGAAGAATAGTGCAGTTAGGGCACAATCAAGTGCTGAATGGTCAGATAGCTCATCTACTGCAGCACAAAAAGACCAACAAGCTAGATTAGATAAGGGAATTTCATCAGGGAATAGGGCATTTAGCCATCCCCATGGAAGTAGTGGCACCACAACTATACAATCAAATGGTAGTTTGTCGGACAGTTCATCCTTGCAGCCAACAAGCTCTCATATGGAAGAGGGCATTAGTTCTTTAGTGATTGGAGGCAAGCCTGAGAGTCTTACTACTAGCTCTAAGAACATGTCTTCCGATGCTAGATCTGGGCATTCGAAAATTGTTAGTTCTAGCGGATCGCATTCACGAGCAGACTATAAACCTGAAAAATGGATGCTTCCTGACAAAGCTGTTGATACATTGACTCAACTGAATCTTGCAATTGTTGGACATGTTGATTCAGGAAAATCAACACTCTCAGGTAGACTGCTTCACCTTTTGGGACGGGTATCTAAAAAAGACATGCACAAATATGAGAAAGAGGCAAAGTCACAGGGCAAGGGGTCATTTGCATATGCTTGGGCATTGGATGAGAGTGCAGAAGAAAGGGAAAGAGGAATAACTATGAATGTGGCTGTTGCATATTTTGATTCCAGAAAGTATCATGTTGTTGTGCTGGATTCTCCTGGCCATAAAGACTTTGTTCCAAACATGATATCTGGGGCAACACAAGCTGATGCTGCAATACTTCTTGTAGATGCTTCGTGTGGTGCATTCGAAGCTGGTATAAAGGGACAGACTATGGAACACGCACAACTTATCAGAAGCTTTGGTGTTGATCAAATTATAGTTGCGGTTAACAAGATGGATGTTGTTGAATACTCCAAGGATCGATTTGATGAAATCAAACAGACACTTGGAACATTTCTCCATTCCCGTGGCTTCAAAGATTCTTCAGTGTATTGGGTTCCATTGAGTGTCATGGAAAATCAAAATTTGGTATCAGCTCCTTCTGAGGCTCGTTTGTTGTCTTGGTATCGTGGACCTTCCCTGTTGGATTCAATTGATTCCCTTCAACCTCCCACGAGAGATTTTCAAAGGCCACTGCTCATGCCAATATGTGATGTCATTAGATCTACTTCTCTGGGACAGGTGTCTGCTTGTGGTAAATTGGAAGCAGGAGCTCTTCGGAGTGGATCAAAGGTTCTGGTTATGCCTTCAGGAGAATTAGGGACTGTCCGCTCTTTAGAACGGGATTCTCAGGCATGTTCCATTGCAAGAGCTGGTGACAATGTGGGTGTTACTCTTCAAGGCATTGATGGTGGTCATGTGATGGCAGGGGGCGTGCTCTGTCACCCTGGTTTTCCGGTTGCAGTTGCAAAACATTTGGAATTGAAAGTGCTTCTTTTGGATGTCGCCACCCCTATTTTAATTGGCTCTCAGGTGGAATTTCACATACACCACGCCAAGGAGGCTGCAAGAGTTGTAAAAATATTGTCATTAATTGATCCAAAGGGAAAAGTGACGAAAAAGTCTCCTCGCTGTCTACTTGCAAAGCAGAGGGCTGTTATAGAGGTAGTTTTGCAAGGACCAGTTTGTGTGGAAGAGTATACAAGTTCTAAAGCTCTAGGGAGCGTATCTCTGAGGGCATCAGGAAACACTGTGGCCCTCGGTGTTGTAAGCCGGATTATAAAGGAGCAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006464 GO:0006468 GO:0006725 GO:0006778 GO:0006787 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0006995 GO:0006996 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009267 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009628 GO:0009635 GO:0009636 GO:0009637 GO:0009639 GO:0009657 GO:0009658 GO:0009668 GO:0009767 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0009987 GO:0009991 GO:0010027 GO:0010033 GO:0010035 GO:0010109 GO:0010114 GO:0010287 GO:0010380 GO:0015979 GO:0015994 GO:0015996 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019216 GO:0019222 GO:0019439 GO:0019538 GO:0019684 GO:0019747 GO:0022900 GO:0031279 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031537 GO:0031540 GO:0031667 GO:0031668 GO:0031669 GO:0033013 GO:0033015 GO:0033554 GO:0034641 GO:0036211 GO:0042221 GO:0042440 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043562 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044270 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045828 GO:0045834 GO:0046149 GO:0046483 GO:0046700 GO:0046889 GO:0046890 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051186 GO:0051187 GO:0051193 GO:0051194 GO:0051716 GO:0055114 GO:0061024 GO:0065007 GO:0065009 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0080177 GO:0080183 GO:0090056 GO:0140096 GO:1901360 GO:1901361 GO:1901401 GO:1901403 GO:1901463 GO:1901465 GO:1901562 GO:1901564 GO:1901565 GO:1901575 GO:1901700 GO:1902171 GO:1902326 GO:1904143
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

1313

Amino Acids

143.75

Weight (kDa)

6.59

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 195 - 439 5.2e-72 ABC1 atypical kinase-like domain
GTP_EFTU PF00009 889 - 1103 9.6e-48 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 1133 - 1196 8.8e-09 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 1204 - 1308 4.1e-10 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 1208 - 1305 9.4e-24 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3926
AasI GACNNNNNNGTC 2 cut(s) 431, 3766
AccB1I GGYRCC 1 cut(s) 2405
AccB7I CCANNNNNTGG 2 cut(s) 1141, 1175
AccBSI CCGCTC 1 cut(s) 3477
AccI GTMKAC 5 cut(s) 305, 847, 2719, 3781, 3845
AccII CGCG 5 cut(s) 28, 30, 176, 187, 279
AccIII TCCGGA 1 cut(s) 141
AcoI YGGCCR 2 cut(s) 520, 2923
AcsI RAATTY 8 cut(s) 4, 887, 1145, 1396, 1562, 2358, 3229, 3683
AcuI CTGAAG 2 cut(s) 3174, 3438
AcvI CACGTG 1 cut(s) 1019
AcyI GRCGYC 1 cut(s) 200
AfaI GTAC 3 cut(s) 765, 1647, 2038
AfiI CCNNNNNNNGG 7 cut(s) 107, 186, 212, 613, 1141, 1175, 3703
AflIII ACRYGT 3 cut(s) 1018, 2541, 2686
AhdI GACNNNNNGTC 1 cut(s) 2230
AjnI CCWGG 5 cut(s) 522, 1174, 1345, 2920, 3592
AjuI GAANNNNNNNTTGG 4 cut(s) 317, 349, 579, 611
AloI GAACNNNNNNTCC 4 cut(s) 2531, 2563, 3423, 3455
Alw21I GWGCWC 9 cut(s) 62, 73, 469, 876, 1058, 1825, 1908, 3421, 3585
Alw26I GTCTC 4 cut(s) 207, 308, 1363, 3774
Alw44I GTGCAC 1 cut(s) 1054
Ama87I CYCGRG 1 cut(s) 311
Aor13HI TCCGGA 1 cut(s) 141
AoxI GGCC 7 cut(s) 131, 500, 520, 770, 2923, 3335, 3900
ApaLI GTGCAC 1 cut(s) 1054
ApoI RAATTY 8 cut(s) 4, 887, 1145, 1396, 1562, 2358, 3229, 3683
AseI ATTAAT 1 cut(s) 3737
Asp700I GAANNNNTTC 2 cut(s) 453, 948
AspLEI GCGC 5 cut(s) 30, 32, 251, 279, 1134
AspS9I GGNCC 7 cut(s) 98, 500, 1013, 1795, 3278, 3824, 3901
AsuC2I CCSGG 1 cut(s) 1619
AsuHPI GGTGA 5 cut(s) 905, 1315, 2720, 3533, 3581
AsuII TTCGAA 4 cut(s) 167, 1432, 2570, 3012
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 5 cut(s) 98, 1013, 1795, 3278, 3824
BaeGI GKGCMC 2 cut(s) 1058, 2284
BalI TGGCCA 2 cut(s) 522, 2925
BanI GGYRCC 1 cut(s) 2405
BanII GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3421
BarI GAAGNNNNNNTAC 4 cut(s) 413, 445, 808, 840
BauI CACGAG 4 cut(s) 1907, 1971, 2601, 3319
BbrPI CACGTG 1 cut(s) 1019
BbsI GAAGAC 2 cut(s) 2226, 2538
Bbv12I GWGCWC 9 cut(s) 62, 73, 469, 876, 1058, 1825, 1908, 3421, 3585
BceAI ACGGC 2 cut(s) 229, 277
BcgI CGANNNNNNTGC 4 cut(s) 339, 373, 1738, 1772
BciT130I CCWGG 5 cut(s) 524, 1176, 1347, 2922, 3594
BclI TGATCA 1 cut(s) 3073
BcnI CCSGG 1 cut(s) 1619
BcoDI GTCTC 4 cut(s) 207, 308, 1363, 3774
BfaI CTAG 8 cut(s) 246, 1025, 1682, 2343, 2529, 2556, 2586, 3863
BfmI CTRYAG 2 cut(s) 848, 2319
BfoI RGCGCY 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 3707
BglII AGATCT 3 cut(s) 1511, 2558, 3368
BlpI GCTNAGC 2 cut(s) 236, 870
BmcAI AGTACT 1 cut(s) 1647
Bme1390I CCNGG 6 cut(s) 524, 1176, 1347, 1619, 2922, 3594
Bme18I GGWCC 5 cut(s) 98, 1013, 1795, 3278, 3824
BmeRI GACNNNNNGTC 1 cut(s) 2230
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 7 cut(s) 98, 500, 1013, 1795, 3278, 3824, 3901
BmiI GGNNCC 5 cut(s) 158, 501, 1344, 2407, 3204
BmrFI CCNGG 6 cut(s) 524, 1176, 1347, 1619, 2922, 3594
BmrI ACTGGG 2 cut(s) 1340, 1452
BmsI GCATC 8 cut(s) 61, 365, 1261, 2542, 2619, 2962, 2983, 3893
BmuI ACTGGG 2 cut(s) 1340, 1452
BoxI GACNNNNGTC 2 cut(s) 1355, 3388
BpiI GAAGAC 2 cut(s) 2226, 2538
BplI GAGNNNNNCTC 2 cut(s) 3859, 3891
Bpu1102I GCTNAGC 2 cut(s) 236, 870
Bpu14I TTCGAA 4 cut(s) 167, 1432, 2570, 3012
BpuEI CTTGAG 3 cut(s) 974, 1509, 1589
BpuMI CCSGG 1 cut(s) 1619
Bsa29I ATCGAT 3 cut(s) 1041, 1269, 3127
BsaAI YACGTR 2 cut(s) 734, 1019
BsaBI GATNNNNATC 3 cut(s) 1170, 1265, 2188
BsaHI GRCGYC 1 cut(s) 200
BsaJI CCNNGG 9 cut(s) 255, 1159, 1346, 2392, 3120, 3172, 3592, 3702, 3904
BsaWI WCCGGW 3 cut(s) 141, 179, 3601
BsaXI ACNNNNNCTCC 3 cut(s) 371, 401, 3923
Bsc4I CCNNNNNNNGG 7 cut(s) 107, 186, 212, 613, 1141, 1175, 3703
Bse118I RCCGGY 2 cut(s) 83, 1082
Bse1I ACTGG 9 cut(s) 702, 745, 1146, 1335, 1447, 1797, 1929, 2214, 3827
Bse3DI GCAATG 3 cut(s) 871, 1739, 3507
Bse8I GATNNNNATC 3 cut(s) 1170, 1265, 2188
BseAI TCCGGA 1 cut(s) 141
BseBI CCWGG 5 cut(s) 524, 1176, 1347, 2922, 3594
BseCI ATCGAT 3 cut(s) 1041, 1269, 3127
BseDI CCNNGG 9 cut(s) 255, 1159, 1346, 2392, 3120, 3172, 3592, 3702, 3904
BseJI GATNNNNATC 3 cut(s) 1170, 1265, 2188
BseLI CCNNNNNNNGG 7 cut(s) 107, 186, 212, 613, 1141, 1175, 3703
BseMI GCAATG 3 cut(s) 871, 1739, 3507
BseNI ACTGG 9 cut(s) 702, 745, 1146, 1335, 1447, 1797, 1929, 2214, 3827
BsePI GCGCGC 1 cut(s) 28
BseRI GAGGAG 3 cut(s) 653, 923, 3762
BseSI GKGCMC 2 cut(s) 1058, 2284
BsgI GTGCAG 3 cut(s) 7, 2292, 2853
Bsh1236I CGCG 5 cut(s) 28, 30, 176, 187, 279
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 7 cut(s) 133, 502, 522, 772, 2925, 3337, 3902
BshNI GGYRCC 1 cut(s) 2405
BshVI ATCGAT 3 cut(s) 1041, 1269, 3127
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 9 cut(s) 62, 73, 469, 876, 1058, 1825, 1908, 3421, 3585
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 8 cut(s) 66, 84, 142, 180, 1083, 1619, 3602, 3919
BslFI GGGAC 5 cut(s) 1363, 2751, 3042, 3396, 3481
BslI CCNNNNNNNGG 7 cut(s) 107, 186, 212, 613, 1141, 1175, 3703
BsmAI GTCTC 4 cut(s) 207, 308, 1363, 3774
BsmBI CGTCTC 1 cut(s) 207
BsmFI GGGAC 5 cut(s) 1363, 2751, 3042, 3396, 3481
BsmI GAATGC 5 cut(s) 175, 579, 2566, 2596, 3008
BsnI GGCC 7 cut(s) 133, 502, 522, 772, 2925, 3337, 3902
BsoBI CYCGRG 1 cut(s) 311
Bsp119I TTCGAA 4 cut(s) 167, 1432, 2570, 3012
Bsp13I TCCGGA 1 cut(s) 141
Bsp1407I TGTACA 1 cut(s) 2036
Bsp1720I GCTNAGC 2 cut(s) 236, 870
Bsp19I CCATGG 3 cut(s) 255, 1159, 2392
BspANI GGCC 7 cut(s) 133, 502, 522, 772, 2925, 3337, 3902
BspDI ATCGAT 3 cut(s) 1041, 1269, 3127
BspEI TCCGGA 1 cut(s) 141
BspFNI CGCG 5 cut(s) 28, 30, 176, 187, 279
BspLI GGNNCC 5 cut(s) 158, 501, 1344, 2407, 3204
BspMAI CTGCAG 1 cut(s) 2323
BspQI GCTCTTC 2 cut(s) 1896, 3426
BspT104I TTCGAA 4 cut(s) 167, 1432, 2570, 3012
BspT107I GGYRCC 1 cut(s) 2405
BsrBI CCGCTC 1 cut(s) 3477
BsrDI GCAATG 3 cut(s) 871, 1739, 3507
BsrFI RCCGGY 2 cut(s) 83, 1082
BsrGI TGTACA 1 cut(s) 2036
BsrI ACTGG 9 cut(s) 702, 745, 1146, 1335, 1447, 1797, 1929, 2214, 3827
BssAI RCCGGY 2 cut(s) 83, 1082
BssECI CCNNGG 9 cut(s) 255, 1159, 1346, 2392, 3120, 3172, 3592, 3702, 3904
BssHII GCGCGC 1 cut(s) 28
BssNAI GTATAC 2 cut(s) 306, 3846
BssNI GRCGYC 1 cut(s) 200
BssSI CACGAG 4 cut(s) 1907, 1971, 2601, 3319
BssT1I CCWWGG 5 cut(s) 255, 1159, 2392, 3120, 3702
Bst1107I GTATAC 2 cut(s) 306, 3846
Bst2BI CACGAG 4 cut(s) 1907, 1971, 2601, 3319
Bst2UI CCWGG 5 cut(s) 524, 1176, 1347, 2922, 3594
Bst6I CTCTTC 6 cut(s) 630, 1896, 2472, 3426, 3546, 3834
BstACI GRCGYC 1 cut(s) 200
BstAUI TGTACA 1 cut(s) 2036
BstBAI YACGTR 2 cut(s) 734, 1019
BstBI TTCGAA 4 cut(s) 167, 1432, 2570, 3012
BstDSI CCRYGG 4 cut(s) 255, 1159, 2392, 3172
BstFNI CGCG 5 cut(s) 28, 30, 176, 187, 279
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 5 cut(s) 30, 32, 251, 279, 1134
BstMAI GTCTC 4 cut(s) 207, 308, 1363, 3774
BstMCI CGRYCG 1 cut(s) 180
BstNI CCWGG 5 cut(s) 524, 1176, 1347, 2922, 3594
BstNSI RCATGY 4 cut(s) 2545, 2690, 2761, 3504
BstPAI GACNNNNGTC 2 cut(s) 1355, 3388
BstSCI CCNGG 6 cut(s) 522, 1174, 1345, 1617, 2920, 3592
BstSFI CTRYAG 2 cut(s) 848, 2319
BstSLI GKGCMC 2 cut(s) 1058, 2284
BstUI CGCG 5 cut(s) 28, 30, 176, 187, 279
BstV2I GAAGAC 2 cut(s) 2226, 2538
BstX2I RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2558, 3368
BstXI CCANNNNNNTGG 2 cut(s) 2393, 3173
BstYI RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2558, 3368
BstZ17I GTATAC 2 cut(s) 306, 3846
Bsu15I ATCGAT 3 cut(s) 1041, 1269, 3127
BsuRI GGCC 7 cut(s) 133, 502, 522, 772, 2925, 3337, 3902
BsuTUI ATCGAT 3 cut(s) 1041, 1269, 3127
BtgI CCRYGG 4 cut(s) 255, 1159, 2392, 3172
BtgZI GCGATG 1 cut(s) 342
BtsI GCAGTG 1 cut(s) 3338
BtsIMutI CAGTG 7 cut(s) 738, 1126, 1800, 2221, 3198, 3338, 3894
CfoI GCGC 5 cut(s) 30, 32, 251, 279, 1134
Cfr10I RCCGGY 2 cut(s) 83, 1082
Cfr13I GGNCC 7 cut(s) 98, 500, 1013, 1795, 3278, 3824, 3901
ClaI ATCGAT 3 cut(s) 1041, 1269, 3127
CseI GACGC 1 cut(s) 189
Csp6I GTAC 3 cut(s) 764, 1646, 2037
CviQI GTAC 3 cut(s) 764, 1646, 2037
DraI TTTAAA 1 cut(s) 964
DrdI GACNNNNNNGTC 2 cut(s) 431, 3766
DriI GACNNNNNGTC 1 cut(s) 2230
DseDI GACNNNNNNGTC 2 cut(s) 431, 3766
EaeI YGGCCR 2 cut(s) 520, 2923
Eam1104I CTCTTC 6 cut(s) 630, 1896, 2472, 3426, 3546, 3834
Eam1105I GACNNNNNGTC 1 cut(s) 2230
EarI CTCTTC 6 cut(s) 630, 1896, 2472, 3426, 3546, 3834
EciI GGCGGA 2 cut(s) 52, 205
Ecl136II GAGCTC 6 cut(s) 60, 71, 874, 1823, 1906, 3419
Eco130I CCWWGG 5 cut(s) 255, 1159, 2392, 3120, 3702
Eco24I GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3421
Eco32I GATATC 1 cut(s) 2952
Eco47I GGWCC 5 cut(s) 98, 1013, 1795, 3278, 3824
Eco53kI GAGCTC 6 cut(s) 60, 71, 874, 1823, 1906, 3419
Eco57I CTGAAG 2 cut(s) 3174, 3438
Eco72I CACGTG 1 cut(s) 1019
Eco88I CYCGRG 1 cut(s) 311
EcoICRI GAGCTC 6 cut(s) 60, 71, 874, 1823, 1906, 3419
EcoO109I RGGNCCY 1 cut(s) 500
EcoRI GAATTC 3 cut(s) 4, 887, 1396
EcoRII CCWGG 5 cut(s) 522, 1174, 1345, 2920, 3592
EcoRV GATATC 1 cut(s) 2952
EcoT14I CCWWGG 5 cut(s) 255, 1159, 2392, 3120, 3702
EcoT38I GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3421
ErhI CCWWGG 5 cut(s) 255, 1159, 2392, 3120, 3702
Esp3I CGTCTC 1 cut(s) 207
FalI AAGNNNNNCTT 4 cut(s) 936, 968, 3624, 3656
FaqI GGGAC 5 cut(s) 1363, 2751, 3042, 3396, 3481
FauI CCCGC 3 cut(s) 52, 143, 941
FauNDI CATATG 4 cut(s) 1585, 2041, 2472, 2809
FbaI TGATCA 1 cut(s) 3073
FblI GTMKAC 5 cut(s) 305, 847, 2719, 3781, 3845
FriOI GRGCYC 6 cut(s) 62, 73, 876, 1825, 1908, 3421
FspBI CTAG 8 cut(s) 246, 1025, 1682, 2343, 2529, 2556, 2586, 3863
GlaI GCGC 5 cut(s) 29, 31, 250, 278, 1133
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 7 cut(s) 133, 502, 522, 772, 2925, 3337, 3902
HapII CCGG 8 cut(s) 66, 84, 142, 180, 1083, 1619, 3602, 3919
HgaI GACGC 1 cut(s) 189
HhaI GCGC 5 cut(s) 30, 32, 251, 279, 1134
Hin1I GRCGYC 1 cut(s) 200
Hin6I GCGC 5 cut(s) 28, 30, 249, 277, 1132
HinP1I GCGC 5 cut(s) 28, 30, 249, 277, 1132
HincII GTYRAC 3 cut(s) 19, 650, 3094
HindII GTYRAC 3 cut(s) 19, 650, 3094
HindIII AAGCTT 3 cut(s) 899, 1889, 3061
HpaI GTTAAC 2 cut(s) 19, 3094
HpaII CCGG 8 cut(s) 66, 84, 142, 180, 1083, 1619, 3602, 3919
HphI GGTGA 5 cut(s) 905, 1315, 2720, 3533, 3581
Hpy99I CGWCG 3 cut(s) 106, 215, 437
HpyAV CCTTC 9 cut(s) 101, 432, 513, 1838, 2048, 3255, 3291, 3317, 3462
HpyCH4IV ACGT 5 cut(s) 733, 993, 1018, 1204, 2226
HpySE526I ACGT 5 cut(s) 733, 993, 1018, 1204, 2226
Hsp92I GRCGYC 1 cut(s) 200
HspAI GCGC 5 cut(s) 28, 30, 249, 277, 1132
Kpn2I TCCGGA 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 3073
KspAI GTTAAC 2 cut(s) 19, 3094
LguI GCTCTTC 2 cut(s) 1896, 3426
LweI GCATC 8 cut(s) 61, 365, 1261, 2542, 2619, 2962, 2983, 3893
MaeI CTAG 8 cut(s) 246, 1025, 1682, 2343, 2529, 2556, 2586, 3863
MaeII ACGT 5 cut(s) 733, 993, 1018, 1204, 2226
MaeIII GTNAC 9 cut(s) 734, 1438, 2069, 2222, 2784, 3521, 3535, 3587, 3757
MbiI CCGCTC 1 cut(s) 3477
MfeI CAATTG 2 cut(s) 2676, 3297
MflI RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2558, 3368
MlsI TGGCCA 2 cut(s) 522, 2925
MluNI TGGCCA 2 cut(s) 522, 2925
MlyI GAGTC 6 cut(s) 147, 1125, 1516, 2240, 2527, 2653
MmeI TCCRAC 9 cut(s) 84, 92, 315, 415, 1093, 2089, 2418, 2662, 3270
Mox20I TGGCCA 2 cut(s) 522, 2925
MroI TCCGGA 1 cut(s) 141
MroXI GAANNNNTTC 2 cut(s) 453, 948
MscI TGGCCA 2 cut(s) 522, 2925
MslI CAYNNNNRTG 3 cut(s) 2391, 2766, 3171
Msp20I TGGCCA 2 cut(s) 522, 2925
MspA1I CMGCKG 1 cut(s) 698
MspI CCGG 8 cut(s) 66, 84, 142, 180, 1083, 1619, 3602, 3919
MspR9I CCNGG 6 cut(s) 524, 1176, 1347, 1619, 2922, 3594
MunI CAATTG 2 cut(s) 2676, 3297
Mva1269I GAATGC 5 cut(s) 175, 579, 2566, 2596, 3008
MvaI CCWGG 5 cut(s) 524, 1176, 1347, 2922, 3594
MvnI CGCG 5 cut(s) 28, 30, 176, 187, 279
NciI CCSGG 1 cut(s) 1619
NcoI CCATGG 3 cut(s) 255, 1159, 2392
NdeI CATATG 4 cut(s) 1585, 2041, 2472, 2809
NlaIV GGNNCC 5 cut(s) 158, 501, 1344, 2407, 3204
NmuCI GTSAC 7 cut(s) 734, 2069, 2222, 2784, 3521, 3587, 3757
NspI RCATGY 4 cut(s) 2545, 2690, 2761, 3504
NspV TTCGAA 4 cut(s) 167, 1432, 2570, 3012
PauI GCGCGC 1 cut(s) 28
PciI ACATGT 2 cut(s) 2541, 2686
PciSI GCTCTTC 2 cut(s) 1896, 3426
PcsI WCGNNNNNNNCGW 4 cut(s) 173, 279, 365, 1275
PctI GAATGC 5 cut(s) 175, 579, 2566, 2596, 3008
PdmI GAANNNNTTC 2 cut(s) 453, 948
PflMI CCANNNNNTGG 2 cut(s) 1141, 1175
PfoI TCCNGGA 1 cut(s) 1174
PleI GAGTC 6 cut(s) 146, 1124, 1516, 2239, 2526, 2653
PmaCI CACGTG 1 cut(s) 1019
PmlI CACGTG 1 cut(s) 1019
PpsI GAGTC 6 cut(s) 146, 1124, 1516, 2239, 2526, 2653
Ppu21I YACGTR 2 cut(s) 734, 1019
PscI ACATGT 2 cut(s) 2541, 2686
PshAI GACNNNNGTC 2 cut(s) 1355, 3388
PshBI ATTAAT 1 cut(s) 3737
PsiI TTATAA 1 cut(s) 3926
Psp124BI GAGCTC 6 cut(s) 62, 73, 876, 1825, 1908, 3421
Psp6I CCWGG 5 cut(s) 522, 1174, 1345, 2920, 3592
PspCI CACGTG 1 cut(s) 1019
PspGI CCWGG 5 cut(s) 522, 1174, 1345, 2920, 3592
PspN4I GGNNCC 5 cut(s) 158, 501, 1344, 2407, 3204
PspPI GGNCC 7 cut(s) 98, 500, 1013, 1795, 3278, 3824, 3901
PsrI GAACNNNNNNTAC 2 cut(s) 1623, 1655
PstI CTGCAG 1 cut(s) 2323
PsuI RGATCY 6 cut(s) 799, 1165, 1511, 1609, 2558, 3368
PteI GCGCGC 1 cut(s) 28
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 3 cut(s) 765, 1647, 2038
RsaNI GTAC 3 cut(s) 764, 1646, 2037
RseI CAYNNNNRTG 3 cut(s) 2391, 2766, 3171
SacI GAGCTC 6 cut(s) 62, 73, 876, 1825, 1908, 3421
SapI GCTCTTC 2 cut(s) 1896, 3426
Sau96I GGNCC 7 cut(s) 98, 500, 1013, 1795, 3278, 3824, 3901
ScaI AGTACT 1 cut(s) 1647
SchI GAGTC 6 cut(s) 147, 1125, 1516, 2240, 2527, 2653
ScrFI CCNGG 6 cut(s) 524, 1176, 1347, 1619, 2922, 3594
SfaNI GCATC 8 cut(s) 61, 365, 1261, 2542, 2619, 2962, 2983, 3893
SfcI CTRYAG 2 cut(s) 848, 2319
SfuI TTCGAA 4 cut(s) 167, 1432, 2570, 3012
SinI GGWCC 5 cut(s) 98, 1013, 1795, 3278, 3824
SmiMI CAYNNNNRTG 3 cut(s) 2391, 2766, 3171
SmlI CTYRAG 3 cut(s) 953, 1524, 1568
SmoI CTYRAG 3 cut(s) 953, 1524, 1568
SspI AATATT 3 cut(s) 940, 2119, 3729
SspMI CTAG 8 cut(s) 246, 1025, 1682, 2343, 2529, 2556, 2586, 3863
SstI GAGCTC 6 cut(s) 62, 73, 876, 1825, 1908, 3421
StyD4I CCNGG 6 cut(s) 522, 1174, 1345, 1617, 2920, 3592
StyI CCWWGG 5 cut(s) 255, 1159, 2392, 3120, 3702
TaiI ACGT 5 cut(s) 736, 996, 1021, 1207, 2229
TaqII GACCGA 1 cut(s) 1892
TatI WGTACW 2 cut(s) 1645, 2036
TauI GCSGC 5 cut(s) 176, 190, 254, 282, 1681
TscAI CASTG 7 cut(s) 745, 1126, 1807, 2221, 3198, 3345, 3901
TseFI GTSAC 7 cut(s) 734, 2069, 2222, 2784, 3521, 3587, 3757
Tsp45I GTSAC 7 cut(s) 734, 2069, 2222, 2784, 3521, 3587, 3757
TspGWI ACGGA 4 cut(s) 27, 591, 803, 1841
TspRI CASTG 7 cut(s) 745, 1126, 1807, 2221, 3198, 3345, 3901
Van91I CCANNNNNTGG 2 cut(s) 1141, 1175
VneI GTGCAC 1 cut(s) 1054
VpaK11BI GGWCC 5 cut(s) 98, 1013, 1795, 3278, 3824
VspI ATTAAT 1 cut(s) 3737
XapI RAATTY 8 cut(s) 4, 887, 1145, 1396, 1562, 2358, 3229, 3683
XceI RCATGY 4 cut(s) 2545, 2690, 2761, 3504
XcmI CCANNNNNNNNNTGG 1 cut(s) 2399
XmiI GTMKAC 5 cut(s) 305, 847, 2719, 3781, 3845
XmnI GAANNNNTTC 2 cut(s) 453, 948
XspI CTAG 8 cut(s) 246, 1025, 1682, 2343, 2529, 2556, 2586, 3863
ZrmI AGTACT 1 cut(s) 1647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.