Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0001871

Physical Location & Seq

Genomic Coordinates Forward (+)

48759478 .. 48773007

Gene Structure 13530 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0335600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7416 bp

ATGGGGAGATTTCTTGGTGGGTTTTTGTTGCCTCTGCTGCTGCTTGCAGCTGCTTTCATGCACTGGAGCTTGATCTCTCTTGGTGATCTCATATCTTTTCTTCTTATTCTATTTCATGCACCCAAAATAGGATTTCATTTTGGAAGGAGGCTTTTATTGTTGTGGTTCATCATCGTATTTTCTTTATGTGCTATTTTTTCTCAACTTCTATATCTGGTTCTATGGGCTATCGAGTGGAGTACAGCAGAAGAGTGGTGGGGAAACCTCATTGGACTCTTGATAGCTGAGTCATGGAAATCACCATCTGTGGTATACTTTACGGTCCTTCAACTCTCCATAGTTGCTGTTTCATTAGTTGATTTGTATGGGAGCAGATTTGGTCCTTTTTCATCATTTGATTCGTGTTGGGGGAAATTCTTATCAACTGTTGATCGTTTAGTCTGTTCTCATATAAGGGTTGCTTCTTGCTTGCTGTTGCCTGCTATTCAACTGGTTGTTGGAATCAGCCGTCCCTCATGGGTTTCTCTACCATTTTTTATTGGGAGCTGTGTTGGTCTTGTGGATTGGTCCTTGACAAGCAATTTTCTAGGACTTTTCAGGTGGTGGAGGCCTTTTCACTTGTATGCAGGCTTCAACATTGTCTTGCTTTATCTGTATCAGCTCCCTGTGGAGTTTCCAGATATGTTACAATGGGTAGCTGATTTCATTGGTCTCTTCAAAATAACTTCAGCTTCCCAATGGACCGAAGTTTGTTCAAGCTTCTCTCTTGTACTTTTTTACATAATGTTATCCTGTGTTAAATGTGATCTAGAGGAGATGGATTTTATTTTGTCTATGAAAGAAAATAACTTGACGGAGCATCTTCTTCCCTCAAAGCATTCATTTTTTATTCGTCAATCTAGATCTGGCGTAAGGCATACCAATGTCATGTTGACAGGAGCAGTTTTCCGGACCTTCAGTATCAACTTTTTCACATATGGTTTTCCGGTATCCTTGGTTGCTCTTTCCTTCTGGAGTTTTCATTTTGCAAGTGTTTGTGCTTTTGGGCTACTTGCATATGTTGGCTACATCATATATGCATTCCCTTCCTTATTCCGTTTGCATCGATTGAATGGGCTGCTTCTTGTCTTCATTCTCTTGTGGGCTGTTAGCACATATATCTTCAATGTAGCTTTCACATTCTTGAATTGGAAAATTGGGAAGAACATGGACATATGGGAGATGGTTGGCTTGTGGCATTATCCCATACCTGGATTCTTTTTGCTTGCACAATTTTGTCTTGGAATGTTGGTTGCTTTGGGTAATCTTGTGAATAACTCAGTTTTCCTTTGCTTGTCTGATGAGGATGGGCGATCATCAACTGACAATAGTACTATTGAAGGTGAGGGAGAGACCAAAGTATTGATTGTTGCCACAATAGCTTGGGGACTGCGCAAAAGCTCTCGGGCTATCATGCTGGCGCTAATATTTCTCATTGCCATGAAACCTGGTTTCATCCATGCTGTGTATGTGATATTCTTTTTGATATATCTTTTGAGCCACAACGTTAACAGAAAATTACGCCAGTCTTTGATTCTCCTATGCGAGGTTCACTTTGCACTATTGTATATCATTCGGATCAATCCGATCTCCAGTAGTTTGGAGCAAGAAGGCTCTTTGAGTGCAGAAGTCTTATTACAGTTAGGTCTCCTTGAAAAGGAAAGCTCGTGGGATTTCTTGGAAATAGCTTTGCTTGCTTGTTTCTGTGCAATTCATAACCATGGTTTTGAAATGCTTTTTTCGTTCTCAGCAATCGTGCAACATACACCTAGCCTTCCATTTGGATTTGGCATTTTGAAAGCTGGTCTGAACAAATCAGTATTGTTGTCAGTGTATGCCTCCTCGTCCACTAATTACAGCCATGACAATCCTTCTTATGAGAGAAGAATAGCATTATTCCTCAGTGCAATCGGGCAGAAGTTCCTATCTATGTACCGATCATGTGGAACCTACATTGCCTTTCTGACTATTCTTGTTAGTGTTTACCTGATGAGACCAAATTATATCTCATTTGGGTACATATCTCTTCTCCTCGTTTGGATAATTGGAAGACAACTCGTTGAGAGAACGAAAAAGCGCTTGTGGTTCCCATTAAAAGCATATTCAATTACAGTGTTCATATTTATCTATAGCTTGAGCAGCTTCCCCAGCATTGAGGTTTGGTTGTCCAGGTTGATAGACCTTGATTTCTATCTGGGGTATGACTCAAAAGCTTCGTCTTTGGAAAATGTTTGGGAATCCCTAGCAGTCTTGATTGTGATGCAACTTTACAGCTACGAGAGGAGGCAGAGCAGGTACAACAGGTCAGATGATCCTGATTTGACAGAATTTGGAGTGCTTGGGTTTGTAAAGCGGTTTCTCATTTGGCACAGCAACAAGATCTTGTTTATTGCATTATTCTATGCATCTTTGTCTCCAATTTGTGCGTTTAGCTTTTTATATCTCCTTGGCCTTGTCATCTGTTCAACTTTACCTAAAGCTTCACGAATCCCGTCTAAATCATTCTTGGCTTACACAGGATTTCTAGTAACAGGAGAGTATCTTTTTCAGATGTTGGGTAGCCAGGCTGCAATGTTTCCTGGACAAAAGCACTCTAATATTGCTCTTCTTTTGGGTTTCCGTGAATTTAAGGCTGGCTTCTGGGGTCTAGAATCTGGTTTGAGAGGAAAAGTGCTGGTGATTGCTGCATGCACTCTTCAGTATAATGTTTTCCACTGGTTGGAGAAGATGCCAAGTACTATTCTAAGCAATGGAATGGGAGAGCCTTGTCCATTGTTTGTGTCAGCAGAAGATACAAATATTAGTGCTCCTATCCCTAGTGAGGATAACAGGCCATCAACTCCACTTTCTGTGAAGCGAGAAGCGGCGAGAAGCCATTCATGGCCATTTTTCTCCCCTGGTCTGTTGCAATCACTCAATCCCTCAACCCCAAAAGCAGGAAGTTCTGAGGGTAGTAGTGGTAGCAAATATTCATTTGGGTATAATATTTGGGGAAGCACCAAGGAGAGCCATAAGTGGAATACGAAAAAAATCCTTGCCTTGCGAAAGGAGAGATTTGAAACACAAAAGCTCATCTCAAAAATCTATATGAAATTCTGGATGGAGAATTTGTTCAATCTCTTTGGTCTTGAGATAAACATGATTGCTCTGCTTCTTGCAAGCTTTGCTTTGTTGAATGCCATATCTATGCTATACATTGCATTGCTTGCTGCTTGTATTATTTTGAATCGGCAAATTATACGCAAGTTATGGCCAATATTCGTCTTCCTGTTTGCTTCCATTCTCGTTCTTGAGTATTTTGCCATCTGGAAGAGTGCTTGGCCTCCAAATCATCCTGATGCAACTAATCCACGTTGCTATGATTGCTGGCAAAAGTCAACCATGTATTTCAGTTACTGCAAGTACTGCTGGTTAGGACTTAATGTTGATGATCCTCGAATGCTTATCAGCTATTTTATGGTCTTCATGCTTGCTTGCTTCAAACTTCGTGCTGATCATTTGTCCAGCTTCTCGGGCTTATCAACGTACCGTGAGATGATATCTCAACGTGAAAATACATTTGTATGGAGAGACCTCTCTTTTGAAACTAAAAGCATGTGGACTTTCCTTGATTATGTGAGGCTTTACTGCTATTGCCATCTATTGGATCTTGTGCTTGCCTTGGTTTTGATTACTGGAACCATTGAGTATGACATCCTGCACCTTGGTTATCTCGCTTTTGCACTGGTTTTCTTTCGGGTTAGACTTCAAATACTAAAGAAAAGGAACAAGATGTTCAAGTACTTGCGCATATACAATTTTGCTCTCATTGTTCTATCTCTTGCATATCAATCTCCTTTTGTGGGTTGTTCTGGGAAGTGTGAGACCATATTCGAGATGATTGGCTTTTATAAATATGACTATGGGTTTCAGATGACTGCAAGATCAGCTCTCGTTGAAATTATCATATTTATGCTGGTATCACTTCAGTCATATATGTTTTCCTCTAAAGAGTTTGATTATGTGTCTCGATATCTTGAAGCTGAACAAATTGGTATGATTGTGCACGAACAAGAAAAAAAAGCTGCATGGAAAACTGCACAATTACAACATATTCGTGAATCTGAGGAGAAGAAACGCCAGCGTAACCTGCAAGTGGAGAAGATGAAGTCAGAGATGCTCAACCTACAAATCCGGCTTCACAGCACGAACTCAGTTACTGACTGCGGTGACTCTTCTCCTGTCAGTGAAGGCCTAAGAAGGAGGAGAAGTACTTCTCTTAACTTGAACAATGATGCTTGGACCCCTGATAAAGAAGGTTTTCCCATGAAAAAGGAGCAGATAATTAGAGAGACTTCTAGCAATGAACTGCATGAATCTCCTGCAACAGGGAATCTGGAAAACCTCACAGTTGCGGATTCTATGAAAAATTCAATGCAATCTATTCATTGTGAGATCACCGAAATTGAAGAGGACGTTGCTGATGGTACATTTTCTGATTCAGAGAAGAAAGAGAAGGGTAAAGGGAAATCAATGGATAACCCTCTAATATCAGCAGTTCATCTGATAGGTGATGGTGTTTCCCAGGTACAGTCCATTGGAAATCAAGCAGTTAACAACCTTGTCAGCTTCTTGAATATTGACCAAGAATCCGATATCCATGAGCACTCTGCTGAGGATGGGGTATATGATGAGATGGAGAGTCAGAAAATAAAGTACGCCAGTTTTCATCGTTCATCTTCCCTGCAGTCTGATATGAGTTCTGATGCCACAAGTCTGCAGTTAGGAAGGATCATCCGTCACATATGGTCCCGAATGCGGTCCAATAATGATGTTGTCTGTTATTGTTGCTTTGTCATCGTCTTTTTGTGGAACTTCAGTTTGCTTTCCATGGTGTATCTGGCAGCTTTATTCTTGTATGCACTGTGTGTTAATTCTGGTCCAAGTTACATATTCTGGGTTATCATGCTCATATACACAGAAGTTTATATTTTGCTTCTGTATCTATACCAGATTATCATCCAGCACTATGGTTCACATATTGCTTCAGACCTACTTCGTGAGTTGGGATTTCCTGTGCGTAAAATGTCTTCATTTGTTGTCAGTTCGTTGCCCCTATTTCTTGTGTACTTATTCACCCTTGTCCAGAGCTCTATAACCGCAAAGGATGGCGAGTGGATGTCTTCAACAGACTTCAACTTCTATAGGAGGAACGCTTTGCAGGGAAAAGAGGTTCCTGTCGGTTATAGCTGGAGTGAGAGGGCAAAGGACCCGCTGCACATAATTGTAAATGTCATACAGTTGATATGTAGAAGCTTCTTTAGGTACTGGGTATCACTGACACAGGGAGCAGAGTCCCCTCCTTACTTTGTTCAGGTTTCTATGGATGTGTGCTCCTGGCCAGAGGATGGGATACAGCCAGAAAGGATTGAGTCTGGAGTAAATCAATTGCTTAGAATCATCCATGATGAAAGATGCAAGGCAAAGGATCCTAAGCGATGCCCTTTTGCTAGCAGGGTGCATGTACAAAGCATTGAAAGGAGTCGAGAAAATGCAAATGTGGCCTTGGTCGTTTTTGAGGTAGTATATGCCTCCCCCATAACAGACTGTGGTTCGGTAGAATGGTACAAGTCACTAACTCCAGCAGCTGATGTGGCAAAGGAGATTCTTAAAGCACTGCATGCTGGCTTTGTTGAAGAAATAGGATTCCCATATCCTATACTCTCTGTTATCGGGGGAGGCAAAAGGGACATTGATCTGTTTGCCTATGTATTTGGTGCTGATCTAAGTGTTTTCTTTTTAGTGGCCATATTCTACCAGTCCATTATAAAAAATAAAAGTGACTTTCTTGACGTGTATCAGCTTGAAGATCAATTTCCTAAAGAGTTCGTCTTCATCTTAATGATTATTTTTTTCTTGATTGTCCTTGATCGAATAATCTACCTCTGTTCATTTGCCACTGGAAAAGTGATTTACTACCTTTTTAACCTCATCCTCTTCACGTATTCAGTTACAAAGTATGCTTGGCATATGGAATCTTCCCGCCAACATGCTGGAGGATTAGCACTCCGTGCTATATTTCTTGCAAAATCAATATCTCTGGCACTGCAAGCTATACAACTTAGACATGGAATTCCTCATAAAAGCACTTTGTACCGTCAATTTTTGACCAGTGAAATTTCACGAATTAATTACGTAGGATATCGACTATATCGTGCTCTGCCGTTCCTTTATGAATTGAGATGTGTACTTGACTGGTCATGTACAACCACATCTTTGACCATGTATGATTGGCTGAAACTGGAGGACATACATGCAAGCTTGTACCTCGTCAAATGTGATGCAGTCCTGAATAGAGCAACGCACAAACAAGGAACAAAGCAAACACAAATGACCAAATGTTGCAATGGGATTTGTCTGTTTTTCATATTGATTTGTGTTATTTGGGCTCCAATGCTGATGTATAGCAGTGGTAACCCAACAAACATTGCAAATCCCATTAAAGATGCCAGTGTTCAAGTTGATATCAAGACAATGAGTGGAAGGTTGACCCTGTATCAGAGCACCCTCTGTGAAAAGATTGATTGGGATAACGTCAACTCTGATGTTAATCTTGACCCTCAAGGTTATTTGGAGACATATAACAAGAAAGATGTCCAGTTGATATGCTGTGAAGCTGATGCAAGTGTTCTGTGGCTTGTTCCTGATGTCGTTCAGACCAGATTCATACGGTCCCTCAATTGGGAGAGCAACATGGCTATAACTTTTACTTGGGTACTTACCAGGGAGAGACCCAAGGGCAAGGAAGTTGTGAAATATGAAAATAATCTTGGTGTTGAGGATCTTCCAAAACAATCAGATGTACAAAAAGTCCTTAATGGCTCTATCAACAGCTTTAGGATACACAATGTCTATCCAAGATACTTGCGTGTCCCAGGTTCTGGTGATGTCAGGCCACTGGAACCAGGGGAAACTTTCGTCACTGCGGACCTCGTTATAAATCGTGCTAGTTACCAGTGGTGGTGGTCCTTCAGTGACATCAATTCATCAGATGTCAACGGATGTGGCGGTTTGAGAGGACCCATGGCCATTATTATGTCTGAAGAAACGCCGCCACAGGGTATTCTTGGTGACACACTAAGCAAGTTCAGCATCTGGGGTCTCTACATAACATTTGTTCTTGCTGTTGGCCGCTTTATCAGACTTCAGTGCTCTGACCTAAGAATGAGAATCCCCTATGAAAATCTTCCCTCCTGTGATAGGTTGTTGGCCATTTGTGAGGATATATATGCTGCAAGAGCAGAGGGTGAGCTTGGTATTGAAGAGATCCTTTACTGGACACTGGTGAAGATTTACAGATCACCACACATGCTGCTAGAGTACACGAAACCTGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000288 GO:0000291 GO:0000956 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019222 GO:0019439 GO:0019538 GO:0023052 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0034641 GO:0034645 GO:0034655 GO:0035639 GO:0036094 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043603 GO:0043604 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0090304 GO:0097159 GO:0097367 GO:1901265 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901566 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03015 ko05134 map03015 map05134

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

2471

Amino Acids

282.63

Weight (kDa)

7.97

Isoelectric Point (pI)

45.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PIEZO	PF25288	469 - 598	3.1e-63	Piezo-type mechanosensitive ion channel homolog domain
THU_Piezo1	PF23188	1614 - 1732	2.5e-15	Piezo1-like, transmembrane helical unit
Piezo_THU9_anchor	PF24874	1925 - 2162	2.7e-65	Piezo, THU9 and anchor domain
Piezo_cap	PF12166	2190 - 2470	1.1e-77	Piezo non-specific cation channel, cap domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001871)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31390 AT4G31390 AT4G31390
fragaria_vesca	FvH4_2g36930 FvH4_2g36930 FvH4_2g36930 FvH4_2g36930 FvH4_2g36930 FvH4_2g36930 FvH4_2g36931 FvH4_2g36931 FvH4_2g36931 FvH4_2g36931 FvH4_2g36931 FvH4_2g36931
malus_domestica	MD08G1131400.v1.1 MD15G1109000.v1.1 MD15G1109100.v1.1
prunus_persica	Prupe.1G461300_v2.0.a1 Prupe.1G461300_v2.0.a1 Prupe.1G461300_v2.0.a1
pyrus_communis	pycom08g11010 pycom15g09950
rosa_chinensis	RchiOBHm_Chr6g0304841 RchiOBHm_Chr6g0304851
rosa_laevigata	RLG00000010945
rosa_multiflora	Rmu_sc0010715.1_g000003 Rmu_sc0010715.1_g000004 Rmu_sc0030552.1_g000002 Rmu_sc0030552.1_g000003 Rmu_ssc0000278.1_g000008
rosa_roxburghii	Rroxscaffold_7G00163410 Rroxscaffold_7G00163420
rosa_rugosa	Rorug06G0335300 Rorug06G0335300 Rorug06G0335300 Rorug06G0335400 Rorug06G0335500 Rorug06G0335600 Rorug06G0335600
rosa_samantha	Rh6AG446900 Rh6BG454700 Rh6CG460400 Rh6DG447400
rosa_wichuraiana	Rw6G038890 Rw6G038900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	3930, 5846, 7016
Acc16I	TGCGCA	2 cut(s)	1433, 3827
Acc36I	ACCTGC	2 cut(s)	2322, 4176
AccB7I	CCANNNNNTGG	4 cut(s)	2758, 3682, 5426, 6959
AccI	GTMKAC	1 cut(s)	312
AccIII	TCCGGA	1 cut(s)	948
AclI	AACGTT	1 cut(s)	1545
AclWI	GGATC	9 cut(s)	1625, 2345, 3464, 3693, 4817, 5501, 5514, 6867, 7339
AcoI	YGGCCR	7 cut(s)	2921, 3290, 5417, 5823, 7104, 7207, 7287
AcsI	RAATTY	8 cut(s)	413, 2366, 2663, 3131, 3145, 4447, 6150, 6195
AcuI	CTGAAG	9 cut(s)	711, 940, 2720, 3989, 4879, 5049, 7033, 7140, 7208
AdeI	CACNNNGTG	2 cut(s)	4946, 6089
AfeI	AGCGCT	1 cut(s)	2117
AflIII	ACRYGT	1 cut(s)	5871
AjiI	CACGTC	1 cut(s)	5872
AjuI	GAANNNNNNNTTGG	2 cut(s)	6831, 6863
AloI	GAACNNNNNNTCC	4 cut(s)	3134, 3166, 5235, 5267
Alw21I	GWGCWC	8 cut(s)	2848, 4086, 4686, 5171, 5414, 6238, 6584, 7232
Alw44I	GTGCAC	1 cut(s)	4082
AlwI	GGATC	9 cut(s)	1625, 2345, 3464, 3693, 4817, 5501, 5514, 6867, 7339
AlwNI	CAGNNNCTG	5 cut(s)	3435, 4238, 5666, 6959, 7173
Ama87I	CYCGRG	2 cut(s)	1443, 3550
Aor13HI	TCCGGA	1 cut(s)	948
Aor51HI	AGCGCT	1 cut(s)	2117
ApaLI	GTGCAC	1 cut(s)	4082
ApoI	RAATTY	8 cut(s)	413, 2366, 2663, 3131, 3145, 4447, 6150, 6195
ArsI	GACNNNNNNTTYG	4 cut(s)	2240, 2272, 3602, 3634
AseI	ATTAAT	1 cut(s)	6207
Asp700I	GAANNNNTTC	5 cut(s)	2914, 3149, 4291, 4338, 7263
AspLEI	GCGC	4 cut(s)	1434, 1462, 2118, 3828
AsuNHI	GCTAGC	1 cut(s)	5528
AvaI	CYCGRG	2 cut(s)	1443, 3550
BaeGI	GKGCMC	1 cut(s)	4086
BaeI	ACNNNNGTAYC	2 cut(s)	6931, 6964
BalI	TGGCCA	6 cut(s)	2923, 3292, 5419, 5825, 7106, 7289
BamHI	GGATCC	1 cut(s)	5506
BanII	GRGCYC	2 cut(s)	5171, 6469
BarI	GAAGNNNNNNTAC	6 cut(s)	614, 646, 1659, 1691, 5092, 5124
BauI	CACGAG	1 cut(s)	1705
BbsI	GAAGAC	7 cut(s)	1120, 2095, 3295, 3493, 5100, 5193, 5902
Bbv12I	GWGCWC	8 cut(s)	2848, 4086, 4686, 5171, 5414, 6238, 6584, 7232
BbvCI	CCTCAGC	1 cut(s)	4692
BceAI	ACGGC	2 cut(s)	492, 6226
BciVI	GTATCC	3 cut(s)	1000, 5424, 6912
BclI	TGATCA	1 cut(s)	3532
BfmI	CTRYAG	4 cut(s)	2167, 4763, 4796, 5221
BfoI	RGCGCY	2 cut(s)	1463, 2119
BfuAI	ACCTGC	2 cut(s)	2322, 4176
BfuI	GTATCC	3 cut(s)	1000, 5424, 6912
BglII	AGATCT	2 cut(s)	902, 2418
BmcAI	AGTACT	5 cut(s)	1372, 2776, 3443, 3821, 4291
BmeT110I	CYCGRG	2 cut(s)	1443, 3550
BmgBI	CACGTC	1 cut(s)	5872
BmrI	ACTGGG	1 cut(s)	5356
BmtI	GCTAGC	1 cut(s)	5532
BmuI	ACTGGG	1 cut(s)	5356
BpiI	GAAGAC	7 cut(s)	1120, 2095, 3295, 3493, 5100, 5193, 5902
BpmI	CTGGAG	8 cut(s)	85, 1033, 1615, 5290, 5475, 5643, 6093, 6341
Bpu10I	CCTNAGC	2 cut(s)	4692, 5511
BpuEI	CTTGAG	4 cut(s)	2194, 3188, 3350, 6624
Bsa29I	ATCGAT	1 cut(s)	1105
BsaAI	YACGTR	2 cut(s)	6021, 6214
BsaBI	GATNNNNATC	3 cut(s)	2229, 5036, 6627
BsaI	GGTCTC	8 cut(s)	716, 1385, 1691, 2026, 3603, 3896, 6802, 7184
BsaWI	WCCGGW	2 cut(s)	948, 985
BsaXI	ACNNNNNCTCC	4 cut(s)	3134, 3164, 4273, 4303
Bse3DI	GCAATG	9 cut(s)	1473, 1992, 2616, 2794, 3234, 3239, 4387, 6430, 6504
Bse8I	GATNNNNATC	3 cut(s)	2229, 5036, 6627
BseAI	TCCGGA	1 cut(s)	948
BseCI	ATCGAT	1 cut(s)	1105
BseJI	GATNNNNATC	3 cut(s)	2229, 5036, 6627
BseMI	GCAATG	9 cut(s)	1473, 1992, 2616, 2794, 3234, 3239, 4387, 6430, 6504
BseMII	CTCAG	8 cut(s)	276, 1332, 1800, 1954, 2976, 4134, 4245, 4683
BseRI	GAGGAG	6 cut(s)	827, 1870, 2060, 2335, 4160, 4297
BseSI	GKGCMC	1 cut(s)	4086
BseYI	CCCAGC	1 cut(s)	2186
BsgI	GTGCAG	4 cut(s)	1683, 3722, 4101, 5279
BshVI	ATCGAT	1 cut(s)	1105
BsiHKAI	GWGCWC	8 cut(s)	2848, 4086, 4686, 5171, 5414, 6238, 6584, 7232
BsiHKCI	CYCGRG	2 cut(s)	1443, 3550
BsiSI	CCGG	3 cut(s)	949, 986, 4213
BslFI	GGGAC	7 cut(s)	495, 1440, 4813, 5359, 5780, 6736, 6935
BsmFI	GGGAC	7 cut(s)	495, 1440, 4813, 5359, 5780, 6736, 6935
BsmI	GAATGC	5 cut(s)	877, 1079, 3220, 3483, 4839
Bso31I	GGTCTC	8 cut(s)	716, 1385, 1691, 2026, 3603, 3896, 6802, 7184
BsoBI	CYCGRG	2 cut(s)	1443, 3550
Bsp1286I	GDGCHC	9 cut(s)	2848, 4086, 4686, 5171, 5414, 6238, 6469, 6584, 7232
Bsp13I	TCCGGA	1 cut(s)	948
Bsp1407I	TGTACA	3 cut(s)	5542, 6281, 6880
Bsp19I	CCATGG	3 cut(s)	1759, 4908, 7101
BspCNI	CTCAG	8 cut(s)	277, 1331, 1799, 1953, 2977, 4135, 4244, 4684
BspDI	ATCGAT	1 cut(s)	1105
BspEI	TCCGGA	1 cut(s)	948
BspMAI	CTGCAG	2 cut(s)	4767, 4800
BspMI	ACCTGC	2 cut(s)	2322, 4176
BspOI	GCTAGC	1 cut(s)	5532
BspPI	GGATC	9 cut(s)	1625, 2345, 3464, 3693, 4817, 5501, 5514, 6867, 7339
BspQI	GCTCTTC	1 cut(s)	2649
BspTNI	GGTCTC	8 cut(s)	716, 1385, 1691, 2026, 3603, 3896, 6802, 7184
BsrDI	GCAATG	9 cut(s)	1473, 1992, 2616, 2794, 3234, 3239, 4387, 6430, 6504
BsrGI	TGTACA	3 cut(s)	5542, 6281, 6880
BssNAI	GTATAC	1 cut(s)	313
BssSI	CACGAG	1 cut(s)	1705
Bst1107I	GTATAC	1 cut(s)	313
Bst2BI	CACGAG	1 cut(s)	1705
BstAPI	GCANNNNNTGC	5 cut(s)	3203, 3245, 3444, 5699, 6089
BstAUI	TGTACA	3 cut(s)	5542, 6281, 6880
BstBAI	YACGTR	2 cut(s)	6021, 6214
BstDSI	CCRYGG	3 cut(s)	1759, 4908, 7101
BstEII	GGTNACC	1 cut(s)	6491
BstENI	CCTNNNNNAGG	4 cut(s)	1583, 1694, 4404, 7276
BstH2I	RGCGCY	2 cut(s)	1463, 2119
BstHHI	GCGC	4 cut(s)	1434, 1462, 2118, 3828
BstNSI	RCATGY	7 cut(s)	2730, 3637, 5543, 5702, 6071, 6335, 7390
BstPI	GGTNACC	1 cut(s)	6491
BstSFI	CTRYAG	4 cut(s)	2167, 4763, 4796, 5221
BstSLI	GKGCMC	1 cut(s)	4086
BstSNI	TACGTA	1 cut(s)	6214
BstV2I	GAAGAC	7 cut(s)	1120, 2095, 3295, 3493, 5100, 5193, 5902
BstX2I	RGATCY	6 cut(s)	902, 2418, 3685, 5506, 6859, 7344
BstXI	CCANNNNNNTGG	3 cut(s)	1639, 5048, 6071
BstYI	RGATCY	6 cut(s)	902, 2418, 3685, 5506, 6859, 7344
BstZ17I	GTATAC	1 cut(s)	313
Bsu15I	ATCGAT	1 cut(s)	1105
BsuI	GTATCC	3 cut(s)	1000, 5424, 6912
BsuTUI	ATCGAT	1 cut(s)	1105
BtgI	CCRYGG	3 cut(s)	1759, 4908, 7101
BtgZI	GCGATG	1 cut(s)	5530
BtrI	CACGTC	1 cut(s)	5872
BtsI	GCAGTG	4 cut(s)	5693, 6122, 6493, 6999
BveI	ACCTGC	2 cut(s)	2322, 4176
CaiI	CAGNNNCTG	5 cut(s)	3435, 4238, 5666, 6959, 7173
CfoI	GCGC	4 cut(s)	1434, 1462, 2118, 3828
ClaI	ATCGAT	1 cut(s)	1105
CsiI	ACCWGGT	1 cut(s)	1486
CspCI	CAANNNNNGTGG	2 cut(s)	7063, 7098
DraIII	CACNNNGTG	2 cut(s)	4946, 6089
EaeI	YGGCCR	7 cut(s)	2921, 3290, 5417, 5823, 7104, 7207, 7287
Ecl136II	GAGCTC	1 cut(s)	5169
Eco105I	TACGTA	1 cut(s)	6214
Eco147I	AGGCCT	2 cut(s)	610, 4272
Eco24I	GRGCYC	2 cut(s)	5171, 6469
Eco31I	GGTCTC	8 cut(s)	716, 1385, 1691, 2026, 3603, 3896, 6802, 7184
Eco32I	GATATC	5 cut(s)	3579, 4052, 4675, 6221, 6544
Eco47III	AGCGCT	1 cut(s)	2117
Eco53kI	GAGCTC	1 cut(s)	5169
Eco57I	CTGAAG	9 cut(s)	711, 940, 2720, 3989, 4879, 5049, 7033, 7140, 7208
Eco88I	CYCGRG	2 cut(s)	1443, 3550
Eco91I	GGTNACC	1 cut(s)	6491
EcoICRI	GAGCTC	1 cut(s)	5169
EcoNI	CCTNNNNNAGG	4 cut(s)	1583, 1694, 4404, 7276
EcoO109I	RGGNCCY	2 cut(s)	5287, 7097
EcoO65I	GGTNACC	1 cut(s)	6491
EcoRI	GAATTC	1 cut(s)	6150
EcoRV	GATATC	5 cut(s)	3579, 4052, 4675, 6221, 6544
EcoT22I	ATGCAT	2 cut(s)	1081, 2446
EcoT38I	GRGCYC	2 cut(s)	5171, 6469
FalI	AAGNNNNNCTT	8 cut(s)	445, 477, 2660, 2692, 3190, 3222, 7332, 7364
FaqI	GGGAC	7 cut(s)	495, 1440, 4813, 5359, 5780, 6736, 6935
FauI	CCCGC	2 cut(s)	5298, 6068
FauNDI	CATATG	5 cut(s)	976, 1057, 1214, 4823, 6048
FbaI	TGATCA	1 cut(s)	3532
FblI	GTMKAC	1 cut(s)	312
FriOI	GRGCYC	2 cut(s)	5171, 6469
FspI	TGCGCA	2 cut(s)	1433, 3827
GlaI	GCGC	4 cut(s)	1433, 1461, 2117, 3827
GsaI	CCCAGC	1 cut(s)	2190
GsuI	CTGGAG	8 cut(s)	85, 1033, 1615, 5290, 5475, 5643, 6093, 6341
HaeII	RGCGCY	2 cut(s)	1463, 2119
HapII	CCGG	3 cut(s)	949, 986, 4213
HhaI	GCGC	4 cut(s)	1434, 1462, 2118, 3828
Hin6I	GCGC	4 cut(s)	1432, 1460, 2116, 3826
HinP1I	GCGC	4 cut(s)	1432, 1460, 2116, 3826
HincII	GTYRAC	7 cut(s)	933, 1549, 3417, 4633, 6567, 6616, 7075
HindII	GTYRAC	7 cut(s)	933, 1549, 3417, 4633, 6567, 6616, 7075
HindIII	AAGCTT	6 cut(s)	757, 2250, 2517, 3199, 5332, 6337
HpaI	GTTAAC	2 cut(s)	1549, 4633
HpaII	CCGG	3 cut(s)	949, 986, 4213
HpyCH4IV	ACGT	9 cut(s)	1545, 3391, 3563, 3586, 4494, 5871, 6020, 6213, 6612
HpySE526I	ACGT	9 cut(s)	1545, 3391, 3563, 3586, 4494, 5871, 6020, 6213, 6612
HspAI	GCGC	4 cut(s)	1432, 1460, 2116, 3826
Kpn2I	TCCGGA	1 cut(s)	948
Ksp22I	TGATCA	1 cut(s)	3532
KspAI	GTTAAC	2 cut(s)	1549, 4633
LguI	GCTCTTC	1 cut(s)	2649
MabI	ACCWGGT	1 cut(s)	1486
MaeII	ACGT	9 cut(s)	1545, 3391, 3563, 3586, 4494, 5871, 6020, 6213, 6612
MfeI	CAATTG	2 cut(s)	5465, 6757
MflI	RGATCY	6 cut(s)	902, 2418, 3685, 5506, 6859, 7344
MhlI	GDGCHC	9 cut(s)	2848, 4086, 4686, 5171, 5414, 6238, 6469, 6584, 7232
MlsI	TGGCCA	6 cut(s)	2923, 3292, 5419, 5825, 7106, 7289
MluNI	TGGCCA	6 cut(s)	2923, 3292, 5419, 5825, 7106, 7289
MlyI	GAGTC	8 cut(s)	267, 296, 2237, 4244, 4729, 5381, 5459, 5569
MmeI	TCCRAC	2 cut(s)	478, 2739
Mox20I	TGGCCA	6 cut(s)	2923, 3292, 5419, 5825, 7106, 7289
Mph1103I	ATGCAT	2 cut(s)	1081, 2446
MroI	TCCGGA	1 cut(s)	948
MroXI	GAANNNNTTC	5 cut(s)	2914, 3149, 4291, 4338, 7263
MscI	TGGCCA	6 cut(s)	2923, 3292, 5419, 5825, 7106, 7289
Msp20I	TGGCCA	6 cut(s)	2923, 3292, 5419, 5825, 7106, 7289
MspA1I	CMGCKG	3 cut(s)	50, 5293, 5666
MspI	CCGG	3 cut(s)	949, 986, 4213
MunI	CAATTG	2 cut(s)	5465, 6757
Mva1269I	GAATGC	5 cut(s)	877, 1079, 3220, 3483, 4839
NcoI	CCATGG	3 cut(s)	1759, 4908, 7101
NdeI	CATATG	5 cut(s)	976, 1057, 1214, 4823, 6048
NheI	GCTAGC	1 cut(s)	5528
NmuCI	GTSAC	7 cut(s)	4247, 4817, 5649, 5858, 6997, 7052, 7148
NsbI	TGCGCA	2 cut(s)	1433, 3827
NsiI	ATGCAT	2 cut(s)	1081, 2446
NspI	RCATGY	7 cut(s)	2730, 3637, 5543, 5702, 6071, 6335, 7390
PaeI	GCATGC	2 cut(s)	2730, 5702
PceI	AGGCCT	2 cut(s)	610, 4272
PciSI	GCTCTTC	1 cut(s)	2649
PcsI	WCGNNNNNNNCGW	1 cut(s)	6229
PctI	GAATGC	5 cut(s)	877, 1079, 3220, 3483, 4839
PdmI	GAANNNNTTC	5 cut(s)	2914, 3149, 4291, 4338, 7263
PflMI	CCANNNNNTGG	4 cut(s)	2758, 3682, 5426, 6959
PfoI	TCCNGGA	1 cut(s)	2617
PleI	GAGTC	8 cut(s)	267, 295, 2237, 4244, 4728, 5380, 5458, 5568
PpsI	GAGTC	8 cut(s)	267, 295, 2237, 4244, 4728, 5380, 5458, 5568
Ppu21I	YACGTR	2 cut(s)	6021, 6214
PpuMI	RGGWCCY	2 cut(s)	5287, 7097
PshBI	ATTAAT	1 cut(s)	6207
PsiI	TTATAA	3 cut(s)	3930, 5846, 7016
Psp124BI	GAGCTC	1 cut(s)	5171
Psp1406I	AACGTT	1 cut(s)	1545
Psp5II	RGGWCCY	2 cut(s)	5287, 7097
PspEI	GGTNACC	1 cut(s)	6491
PspFI	CCCAGC	1 cut(s)	2186
PspPPI	RGGWCCY	2 cut(s)	5287, 7097
PstI	CTGCAG	2 cut(s)	4767, 4800
PstNI	CAGNNNCTG	5 cut(s)	3435, 4238, 5666, 6959, 7173
PsuI	RGATCY	6 cut(s)	902, 2418, 3685, 5506, 6859, 7344
PvuII	CAGCTG	2 cut(s)	50, 5666
SacI	GAGCTC	1 cut(s)	5171
SapI	GCTCTTC	1 cut(s)	2649
ScaI	AGTACT	5 cut(s)	1372, 2776, 3443, 3821, 4291
SchI	GAGTC	8 cut(s)	267, 296, 2237, 4244, 4729, 5381, 5459, 5569
SduI	GDGCHC	9 cut(s)	2848, 4086, 4686, 5171, 5414, 6238, 6469, 6584, 7232
SexAI	ACCWGGT	1 cut(s)	1486
SfcI	CTRYAG	4 cut(s)	2167, 4763, 4796, 5221
SmlI	CTYRAG	4 cut(s)	2173, 3167, 3329, 6639
SmoI	CTYRAG	4 cut(s)	2173, 3167, 3329, 6639
SnaBI	TACGTA	1 cut(s)	6214
SphI	GCATGC	2 cut(s)	2730, 5702
SseBI	AGGCCT	2 cut(s)	610, 4272
SspI	AATATT	7 cut(s)	1467, 2638, 2839, 3008, 3025, 3297, 4657
SstI	GAGCTC	1 cut(s)	5171
StuI	AGGCCT	2 cut(s)	610, 4272
TaiI	ACGT	9 cut(s)	1548, 3394, 3566, 3589, 4497, 5874, 6023, 6216, 6615
TaqI	TCGA	8 cut(s)	231, 1105, 3475, 3912, 4048, 5563, 5950, 6223
TaqII	GACCGA	1 cut(s)	758
TauI	GCSGC	3 cut(s)	2906, 7132, 7212
TseFI	GTSAC	7 cut(s)	4247, 4817, 5649, 5858, 6997, 7052, 7148
Tsp45I	GTSAC	7 cut(s)	4247, 4817, 5649, 5858, 6997, 7052, 7148
TspGWI	ACGGA	6 cut(s)	869, 1085, 2648, 4805, 6077, 7092
Van91I	CCANNNNNTGG	4 cut(s)	2758, 3682, 5426, 6959
VneI	GTGCAC	1 cut(s)	4082
VspI	ATTAAT	1 cut(s)	6207
XagI	CCTNNNNNAGG	4 cut(s)	1583, 1694, 4404, 7276
XapI	RAATTY	8 cut(s)	413, 2366, 2663, 3131, 3145, 4447, 6150, 6195
XbaI	TCTAGA	3 cut(s)	808, 899, 2686
XceI	RCATGY	7 cut(s)	2730, 3637, 5543, 5702, 6071, 6335, 7390
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4915
XmiI	GTMKAC	1 cut(s)	312
XmnI	GAANNNNTTC	5 cut(s)	2914, 3149, 4291, 4338, 7263
ZrmI	AGTACT	5 cut(s)	1372, 2776, 3443, 3821, 4291
Zsp2I	ATGCAT	2 cut(s)	1081, 2446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.