Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001871

Physical Location & Seq

Genomic Coordinates Reverse (-)

7598219 .. 7604437

Gene Structure 6219 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1109000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2109 bp

ATGTTGCTGATTTTGACCATGCCTCGTAAAGTAAACTACGGAATTGATTATGATGACGGGTACGATGCTTATGAAGATCATGATTATGATTATGATGATGATGATGAGTACGGAATAGAAAAGAATGTAACAGGCAAAGCAGTTGAGTCAAAGCGCGAAACTGAAAGGCCTAAGGTCTGGAGTTGTTCCATTTGCACGTATGATAATGAAGAGAATACGTCTGCATGTGACATTTGTGGGGTTCTTCGCAACCCTTTGTCTAAAAGCAATAGAGATAATAAAACAGCTCCTTTCAAGTTTGATGTTCCATCCCCAGATGACGTGGTTTATAATGGATTGTGGTCCTCCAAAACAGGTTCAAAAGCTGGTGTTATTGACTTGAAATCTTCAAAAGCTTCCCCAAATGTTGCGAAGAAGAGTAGTGCAGTTAGTGCACAATCAAGTGCTGAATGGTCAGGTAGTTCATCTACAATGGCACAAAAAAGGGAACAAGGTAGATTAGATAAGGAAATTCAGTTGGAGAATCAGACAGTTAGTTATCACCATGGAAGTAGTGGCGCGATTACTATACAAGAAAATGGTAAATTTTCAGAGAGTTCATCTGCTTCGCAGCCAACAAGCAGACGTGACAGTGGGGTTGAGAGCACTTCTGAAATTAGGGGCAAGTCACAGAGTGTTACCAGTGGCTTGAAAAACATGACTTTGGATGCTCGATCTGGATATTCTAATAGTGTTAGTGTTAGAGAATCTCGTTCACAAGCGGACTATAAACCTGAGAAGTGGATGCTTCCTGACCAAGCTATAGATACACTGACTCAACTGAATCTTGCAATAGTTGGTCATGTTGATTCTGGAAAATCAACACTCTCTGGTAGGTTGCTTCACCTTTTGGGAAGGATATCTCAGAAAGAAATGCACAAATATGAGAAAGAGGCAAACTTGCAGGGAAAGGGGTCCTTCGCATATGCTTGGGCATTGGATGAGAGTACCGAAGAAAGGGAAAGAGGAATAACTATGACAGTGGCTGTTGCATACTTTGATTCCAGAAAATATCATGTTGTTGTGCTCGACTCTCCTGGCCATAAAGATTTTGTTCCTAACATGATATCTGGGGCAACACAAGCTGATGCTGCAATACTTCTCATAGATGCCTCTGTTGGTGCATTCGAAGCTGGTATGGATGTTGGCAAGGGACAGACAAGGGAGCATGCACAACTTATTAGAAGTTTTGGTGTTGATCAAATTATAGTTGCTATTAACAAGATGGATATTGTTGAGTACTCCAAGGATCGATTTGATTTGATCAGACAGACACTTGGAACATTTCTCCGTTCCTGTGGTTTCAGAGATTCTTCGGTGTCTTGGATTCCATTGAGTGCCATGGAAAATCAAAATTTGGTAGCAGCTCCTTCTGATGTCCGTTTATCGTCTTGGTACCGTGGACCTTACCTGTTGGATGCAATTGATTCCCTCAAACCTCCTACAAGAGAGTTTTCAAAGCCATTGCTGATGCCTATATGTGATGTCATCAAATCTTCTTCACAAGGACAGGTGTCTGCTTGTGGTAAATTGGAAGCAGGAGCTCTTCGAAGTGGATTCAAGGTTCTGGTTATGCCTTCGGGAGAATCAGGGACTGTACGCTTGTTAGAACGTGACTCTCAGGCTTGTGCAATTGCAAGGGCTGGGGACAATGTGGCTGTTACTCTGCAAGGCATTGATGGAGGTCGTGTGATGGCAGGAGGTGTGCTCTGTCACCCTAGTTTTCCGGTTGCAGTTGCAAAACATTTGGAATTGAAGGTGCTTGTTTTGGATGTTACAACCCCAATTTTAATTGGTTCTCAGTTGGAATTCCACATACACCACGCGAAGGAGGCTGCGAGAGTGGTAAAGATTTCATCATTGCTTGATCCAAAGACTGGAAAACTGACAAGAAAGGCTCCTCGCTGTCTTACTGCAAAGCAGAGCGCGGTCGTAGAGGTCAGTCTTCATGCACCGGTTTGTGTTGAAGAGTTCTCCAACTCCAGAGCCCTTGGGAGGGCCTTTTTAAGGGCGTTAGGAAGCACCATTGCTGTCGGTGTTGTAACCCGGATTATAGAGGAGCAGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0000288 GO:0000291 GO:0000956 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019222 GO:0019439 GO:0019538 GO:0023052 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0034641 GO:0034645 GO:0034655 GO:0035639 GO:0036094 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043603 GO:0043604 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0071704 GO:0090304 GO:0097159 GO:0097367 GO:1901265 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901566 GO:1901575 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03015 ko05134 map03015 map05134

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

703

Amino Acids

76.45

Weight (kDa)

6.6

Isoelectric Point (pI)

46.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	274 - 491	3.1e-48	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	521 - 584	9.6e-06	Elongation factor Tu domain 2
GTP_EFTU_D3	PF03143	590 - 697	7.3e-11	Elongation factor Tu C-terminal domain
GTP-eEF1A_C	PF22594	596 - 695	9.1e-27	GTP-eEF1A C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001871)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31390 AT4G31390 AT4G31390
fragaria_vesca	FvH4_2g36930 FvH4_2g36930 FvH4_2g36930 FvH4_2g36930 FvH4_2g36930 FvH4_2g36930 FvH4_2g36931 FvH4_2g36931 FvH4_2g36931 FvH4_2g36931 FvH4_2g36931 FvH4_2g36931
malus_domestica	MD08G1131400.v1.1 MD15G1109000.v1.1 MD15G1109100.v1.1
prunus_persica	Prupe.1G461300_v2.0.a1 Prupe.1G461300_v2.0.a1 Prupe.1G461300_v2.0.a1
pyrus_communis	pycom08g11010 pycom15g09950
rosa_chinensis	RchiOBHm_Chr6g0304841 RchiOBHm_Chr6g0304851
rosa_laevigata	RLG00000010945
rosa_multiflora	Rmu_sc0010715.1_g000003 Rmu_sc0010715.1_g000004 Rmu_sc0030552.1_g000002 Rmu_sc0030552.1_g000003 Rmu_ssc0000278.1_g000008
rosa_roxburghii	Rroxscaffold_7G00163410 Rroxscaffold_7G00163420
rosa_rugosa	Rorug06G0335300 Rorug06G0335300 Rorug06G0335300 Rorug06G0335400 Rorug06G0335500 Rorug06G0335600 Rorug06G0335600
rosa_samantha	Rh6AG446900 Rh6BG454700 Rh6CG460400 Rh6DG447400
rosa_wichuraiana	Rw6G038890 Rw6G038900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	330
Acc65I	GGTACC	1 cut(s)	1434
AccB1I	GGYRCC	1 cut(s)	1434
AccB7I	CCANNNNNTGG	1 cut(s)	1916
AccII	CGCG	4 cut(s)	156, 560, 1865, 1967
AciI	CCGC	2 cut(s)	761, 1967
AclWI	GGATC	2 cut(s)	1296, 1901
AcoI	YGGCCR	1 cut(s)	1078
AcsI	RAATTY	4 cut(s)	510, 584, 1393, 1847
AdeI	CACNNNGTG	1 cut(s)	674
AfaI	GTAC	6 cut(s)	62, 110, 988, 1280, 1436, 1638
AfiI	CCNNNNNNNGG	6 cut(s)	996, 1867, 1916, 2034, 2035, 2046
AgeI	ACCGGT	1 cut(s)	1993
AgsI	TTSAA	9 cut(s)	295, 360, 382, 390, 691, 1497, 1600, 1795, 2006
AjiI	CACGTC	2 cut(s)	322, 626
AjnI	CCWGG	1 cut(s)	1075
AloI	GAACNNNNNNTCC	2 cut(s)	1587, 1619
AluBI	AGCT	8 cut(s)	287, 365, 395, 800, 1124, 1172, 1406, 1583
AluI	AGCT	8 cut(s)	287, 365, 395, 800, 1124, 1172, 1406, 1583
Alw21I	GWGCWC	5 cut(s)	436, 647, 1068, 1585, 1749
Alw44I	GTGCAC	1 cut(s)	432
AlwI	GGATC	2 cut(s)	1296, 1901
AlwNI	CAGNNNCTG	2 cut(s)	1025, 1634
AoxI	GGCC	3 cut(s)	167, 1078, 2037
ApaLI	GTGCAC	1 cut(s)	432
ApeKI	GCWGC	4 cut(s)	610, 1130, 1403, 1874
ApoI	RAATTY	4 cut(s)	510, 584, 1393, 1847
AsiGI	ACCGGT	1 cut(s)	1993
Asp718I	GGTACC	1 cut(s)	1434
AspLEI	GCGC	3 cut(s)	156, 560, 1967
AspS9I	GGNCC	4 cut(s)	342, 954, 1442, 2037
AsuC2I	CCSGG	1 cut(s)	2086
AsuHPI	GGTGA	3 cut(s)	533, 875, 1745
AsuII	TTCGAA	2 cut(s)	1167, 1588
AvaII	GGWCC	3 cut(s)	342, 954, 1442
AxyI	CCTNAGG	1 cut(s)	171
BaeGI	GKGCMC	1 cut(s)	436
BalI	TGGCCA	1 cut(s)	1080
BanI	GGYRCC	1 cut(s)	1434
BanII	GRGCYC	2 cut(s)	1585, 2029
BbsI	GAAGAC	1 cut(s)	1976
Bbv12I	GWGCWC	5 cut(s)	436, 647, 1068, 1585, 1749
BbvI	GCAGC	4 cut(s)	622, 1117, 1415, 1861
BccI	CCATC	4 cut(s)	316, 1258, 1712, 1726
BciT130I	CCWGG	1 cut(s)	1077
BclI	TGATCA	2 cut(s)	1237, 1302
BcnI	CCSGG	1 cut(s)	2086
BfaI	CTAG	1 cut(s)	1758
BfmI	CTRYAG	1 cut(s)	801
BisI	GCNGC	4 cut(s)	611, 1131, 1404, 1875
BlsI	GCNGC	4 cut(s)	612, 1132, 1405, 1876
BmcAI	AGTACT	1 cut(s)	1280
Bme1390I	CCNGG	2 cut(s)	1077, 2086
Bme18I	GGWCC	3 cut(s)	342, 954, 1442
BmgBI	CACGTC	2 cut(s)	322, 626
BmgT120I	GGNCC	4 cut(s)	342, 954, 1442, 2037
BmiI	GGNNCC	3 cut(s)	955, 1436, 1938
BmrFI	CCNGG	2 cut(s)	1077, 2086
BmsI	GCATC	7 cut(s)	55, 697, 774, 1117, 1138, 1447, 1500
BoxI	GACNNNNGTC	1 cut(s)	1552
BpiI	GAAGAC	1 cut(s)	1976
BplI	GAGNNNNNCTC	2 cut(s)	1731, 1763
BpmI	CTGGAG	2 cut(s)	199, 2005
Bpu14I	TTCGAA	2 cut(s)	1167, 1588
BpuMI	CCSGG	1 cut(s)	2086
Bsa29I	ATCGAT	1 cut(s)	1291
BsaAI	YACGTR	1 cut(s)	198
BsaJI	CCNNGG	5 cut(s)	544, 1284, 1380, 1438, 2029
BsaWI	WCCGGW	2 cut(s)	1765, 1993
BsaXI	ACNNNNNCTCC	3 cut(s)	1713, 1743, 2090
Bsc4I	CCNNNNNNNGG	6 cut(s)	996, 1867, 1916, 2034, 2035, 2046
Bse118I	RCCGGY	1 cut(s)	1993
Bse1I	ACTGG	2 cut(s)	681, 1921
Bse21I	CCTNAGG	1 cut(s)	171
Bse3DI	GCAATG	3 cut(s)	1502, 1898, 2064
BseBI	CCWGG	1 cut(s)	1077
BseCI	ATCGAT	1 cut(s)	1291
BseDI	CCNNGG	5 cut(s)	544, 1284, 1380, 1438, 2029
BseGI	GGATG	7 cut(s)	308, 712, 789, 985, 1186, 1462, 1816
BseLI	CCNNNNNNNGG	6 cut(s)	996, 1867, 1916, 2034, 2035, 2046
BseMI	GCAATG	3 cut(s)	1502, 1898, 2064
BseMII	CTCAG	4 cut(s)	765, 917, 1673, 1853
BseNI	ACTGG	2 cut(s)	681, 1921
BseRI	GAGGAG	1 cut(s)	1929
BseSI	GKGCMC	1 cut(s)	436
BseXI	GCAGC	4 cut(s)	622, 1117, 1415, 1861
BseYI	CCCAGC	1 cut(s)	1682
BsgI	GTGCAG	1 cut(s)	444
Bsh1236I	CGCG	4 cut(s)	156, 560, 1865, 1967
Bsh1285I	CGRYCG	1 cut(s)	1971
BshFI	GGCC	3 cut(s)	169, 1080, 2039
BshNI	GGYRCC	1 cut(s)	1434
BshTI	ACCGGT	1 cut(s)	1993
BshVI	ATCGAT	1 cut(s)	1291
BsiEI	CGRYCG	1 cut(s)	1971
BsiHKAI	GWGCWC	5 cut(s)	436, 647, 1068, 1585, 1749
BsiSI	CCGG	3 cut(s)	1766, 1994, 2086
BslFI	GGGAC	3 cut(s)	1206, 1645, 1700
BslI	CCNNNNNNNGG	6 cut(s)	996, 1867, 1916, 2034, 2035, 2046
BsmFI	GGGAC	3 cut(s)	1206, 1645, 1700
BsmI	GAATGC	1 cut(s)	1163
BsnI	GGCC	3 cut(s)	169, 1080, 2039
Bsp119I	TTCGAA	2 cut(s)	1167, 1588
Bsp1286I	GDGCHC	6 cut(s)	436, 647, 1068, 1585, 1749, 2029
Bsp143I	GATC	6 cut(s)	76, 713, 1237, 1288, 1302, 1906
Bsp19I	CCATGG	2 cut(s)	544, 1380
BspACI	CCGC	2 cut(s)	761, 1967
BspANI	GGCC	3 cut(s)	169, 1080, 2039
BspCNI	CTCAG	4 cut(s)	766, 916, 1672, 1852
BspDI	ATCGAT	1 cut(s)	1291
BspFNI	CGCG	4 cut(s)	156, 560, 1865, 1967
BspHI	TCATGA	1 cut(s)	79
BspLI	GGNNCC	3 cut(s)	955, 1436, 1938
BspPI	GGATC	2 cut(s)	1296, 1901
BspQI	GCTCTTC	1 cut(s)	1590
BspT104I	TTCGAA	2 cut(s)	1167, 1588
BspT107I	GGYRCC	1 cut(s)	1434
BsrDI	GCAATG	3 cut(s)	1502, 1898, 2064
BsrFI	RCCGGY	1 cut(s)	1993
BsrI	ACTGG	2 cut(s)	681, 1921
BssAI	RCCGGY	1 cut(s)	1993
BssECI	CCNNGG	5 cut(s)	544, 1284, 1380, 1438, 2029
BssMI	GATC	6 cut(s)	76, 713, 1237, 1288, 1302, 1906
BssT1I	CCWWGG	4 cut(s)	544, 1284, 1380, 2029
Bst2UI	CCWGG	1 cut(s)	1077
Bst4CI	ACNGT	5 cut(s)	532, 632, 1021, 1439, 1636
Bst6I	CTCTTC	4 cut(s)	204, 410, 1590, 2001
BstAPI	GCANNNNNTGC	1 cut(s)	431
BstBAI	YACGTR	1 cut(s)	198
BstBI	TTCGAA	2 cut(s)	1167, 1588
BstC8I	GCNNGC	1 cut(s)	1209
BstDEI	CTNAG	5 cut(s)	171, 774, 903, 1659, 1839
BstDSI	CCRYGG	3 cut(s)	544, 1380, 1438
BstENI	CCTNNNNNAGG	1 cut(s)	2044
BstF5I	GGATG	7 cut(s)	308, 712, 789, 985, 1186, 1462, 1816
BstFNI	CGCG	4 cut(s)	156, 560, 1865, 1967
BstHHI	GCGC	3 cut(s)	156, 560, 1967
BstKTI	GATC	6 cut(s)	79, 716, 1240, 1291, 1305, 1909
BstMBI	GATC	6 cut(s)	76, 713, 1237, 1288, 1302, 1906
BstMCI	CGRYCG	1 cut(s)	1971
BstMWI	GCNNNNNNNGC	5 cut(s)	431, 1121, 1130, 1169, 1871
BstNI	CCWGG	1 cut(s)	1077
BstNSI	RCATGY	2 cut(s)	228, 1211
BstPAI	GACNNNNGTC	1 cut(s)	1552
BstSCI	CCNGG	2 cut(s)	1075, 2084
BstSFI	CTRYAG	1 cut(s)	801
BstSLI	GKGCMC	1 cut(s)	436
BstUI	CGCG	4 cut(s)	156, 560, 1865, 1967
BstV1I	GCAGC	4 cut(s)	622, 1117, 1415, 1861
BstV2I	GAAGAC	1 cut(s)	1976
Bsu15I	ATCGAT	1 cut(s)	1291
Bsu36I	CCTNAGG	1 cut(s)	171
BsuRI	GGCC	3 cut(s)	169, 1080, 2039
BsuTUI	ATCGAT	1 cut(s)	1291
BtgI	CCRYGG	3 cut(s)	544, 1380, 1438
BtrI	CACGTC	2 cut(s)	322, 626
BtsCI	GGATG	7 cut(s)	308, 712, 789, 985, 1186, 1462, 1816
BtsIMutI	CAGTG	4 cut(s)	637, 688, 809, 1026
Cac8I	GCNNGC	1 cut(s)	1209
CaiI	CAGNNNCTG	2 cut(s)	1025, 1634
CciI	TCATGA	1 cut(s)	79
CfoI	GCGC	3 cut(s)	156, 560, 1967
Cfr10I	RCCGGY	1 cut(s)	1993
Cfr13I	GGNCC	4 cut(s)	342, 954, 1442, 2037
ClaI	ATCGAT	1 cut(s)	1291
Csp6I	GTAC	6 cut(s)	61, 109, 987, 1279, 1435, 1637
CspAI	ACCGGT	1 cut(s)	1993
CviQI	GTAC	6 cut(s)	61, 109, 987, 1279, 1435, 1637
DdeI	CTNAG	5 cut(s)	171, 774, 903, 1659, 1839
DpnI	GATC	6 cut(s)	78, 715, 1239, 1290, 1304, 1908
DpnII	GATC	6 cut(s)	76, 713, 1237, 1288, 1302, 1906
DraIII	CACNNNGTG	1 cut(s)	674
EaeI	YGGCCR	1 cut(s)	1078
Eam1104I	CTCTTC	4 cut(s)	204, 410, 1590, 2001
EarI	CTCTTC	4 cut(s)	204, 410, 1590, 2001
Ecl136II	GAGCTC	1 cut(s)	1583
Eco130I	CCWWGG	4 cut(s)	544, 1284, 1380, 2029
Eco147I	AGGCCT	1 cut(s)	169
Eco24I	GRGCYC	2 cut(s)	1585, 2029
Eco32I	GATATC	2 cut(s)	900, 1107
Eco47I	GGWCC	3 cut(s)	342, 954, 1442
Eco53kI	GAGCTC	1 cut(s)	1583
Eco81I	CCTNAGG	1 cut(s)	171
EcoICRI	GAGCTC	1 cut(s)	1583
EcoNI	CCTNNNNNAGG	1 cut(s)	2044
EcoO109I	RGGNCCY	2 cut(s)	954, 2037
EcoRI	GAATTC	1 cut(s)	1847
EcoRII	CCWGG	1 cut(s)	1075
EcoRV	GATATC	2 cut(s)	900, 1107
EcoT14I	CCWWGG	4 cut(s)	544, 1284, 1380, 2029
EcoT38I	GRGCYC	2 cut(s)	1585, 2029
ErhI	CCWWGG	4 cut(s)	544, 1284, 1380, 2029
FalI	AAGNNNNNCTT	2 cut(s)	941, 973
FaqI	GGGAC	3 cut(s)	1206, 1645, 1700
FauNDI	CATATG	1 cut(s)	964
FbaI	TGATCA	2 cut(s)	1237, 1302
Fnu4HI	GCNGC	4 cut(s)	611, 1131, 1404, 1875
FokI	GGATG	7 cut(s)	295, 719, 796, 992, 1193, 1469, 1823
FriOI	GRGCYC	2 cut(s)	1585, 2029
Fsp4HI	GCNGC	4 cut(s)	611, 1131, 1404, 1875
FspBI	CTAG	1 cut(s)	1758
GlaI	GCGC	3 cut(s)	155, 559, 1966
GluI	GCNGC	4 cut(s)	611, 1131, 1404, 1875
GsaI	CCCAGC	1 cut(s)	1686
GsuI	CTGGAG	2 cut(s)	199, 2005
HaeIII	GGCC	3 cut(s)	169, 1080, 2039
HapII	CCGG	3 cut(s)	1766, 1994, 2086
HhaI	GCGC	3 cut(s)	156, 560, 1967
Hin6I	GCGC	3 cut(s)	154, 558, 1965
HinP1I	GCGC	3 cut(s)	154, 558, 1965
HindIII	AAGCTT	1 cut(s)	393
HpaII	CCGG	3 cut(s)	1766, 1994, 2086
HphI	GGTGA	3 cut(s)	533, 875, 1745
Hpy166II	GTNNAC	4 cut(s)	34, 434, 755, 1442
Hpy188I	TCNGA	7 cut(s)	528, 592, 652, 906, 1307, 1346, 1414
Hpy188III	TCNNGA	8 cut(s)	80, 178, 717, 791, 852, 1044, 1620, 2022
Hpy8I	GTNNAC	4 cut(s)	34, 434, 755, 1442
HpyAV	CCTTC	6 cut(s)	888, 967, 1419, 1626, 1789, 1861
HpyCH4III	ACNGT	5 cut(s)	532, 632, 1021, 1439, 1636
HpyCH4IV	ACGT	5 cut(s)	197, 218, 321, 625, 1651
HpyF10VI	GCNNNNNNNGC	5 cut(s)	431, 1121, 1130, 1169, 1871
HpyF3I	CTNAG	5 cut(s)	171, 774, 903, 1659, 1839
HpySE526I	ACGT	5 cut(s)	197, 218, 321, 625, 1651
HspAI	GCGC	3 cut(s)	154, 558, 1965
KpnI	GGTACC	1 cut(s)	1438
Ksp22I	TGATCA	2 cut(s)	1237, 1302
Kzo9I	GATC	6 cut(s)	76, 713, 1237, 1288, 1302, 1906
LguI	GCTCTTC	1 cut(s)	1590
LmnI	GCTCC	6 cut(s)	292, 1204, 1411, 1580, 1942, 2098
Lsp1109I	GCAGC	4 cut(s)	622, 1117, 1415, 1861
LweI	GCATC	7 cut(s)	55, 697, 774, 1117, 1138, 1447, 1500
MaeI	CTAG	1 cut(s)	1758
MaeII	ACGT	5 cut(s)	197, 218, 321, 625, 1651
MalI	GATC	6 cut(s)	78, 715, 1239, 1290, 1304, 1908
MboI	GATC	6 cut(s)	76, 713, 1237, 1288, 1302, 1906
MfeI	CAATTG	2 cut(s)	1461, 1671
MhlI	GDGCHC	6 cut(s)	436, 647, 1068, 1585, 1749, 2029
MlsI	TGGCCA	1 cut(s)	1080
MluNI	TGGCCA	1 cut(s)	1080
MlyI	GAGTC	4 cut(s)	155, 808, 1064, 1649
MmeI	TCCRAC	4 cut(s)	498, 1434, 1824, 2040
Mox20I	TGGCCA	1 cut(s)	1080
MscI	TGGCCA	1 cut(s)	1080
MseI	TTAA	3 cut(s)	1257, 1829, 2045
MslI	CAYNNNNRTG	2 cut(s)	84, 921
Msp20I	TGGCCA	1 cut(s)	1080
MspI	CCGG	3 cut(s)	1766, 1994, 2086
MspR9I	CCNGG	2 cut(s)	1077, 2086
MunI	CAATTG	2 cut(s)	1461, 1671
Mva1269I	GAATGC	1 cut(s)	1163
MvaI	CCWGG	1 cut(s)	1077
MvnI	CGCG	4 cut(s)	156, 560, 1865, 1967
MwoI	GCNNNNNNNGC	5 cut(s)	431, 1121, 1130, 1169, 1871
NciI	CCSGG	1 cut(s)	2086
NcoI	CCATGG	2 cut(s)	544, 1380
NdeI	CATATG	1 cut(s)	964
NdeII	GATC	6 cut(s)	76, 713, 1237, 1288, 1302, 1906
NlaIV	GGNNCC	3 cut(s)	955, 1436, 1938
NmuCI	GTSAC	5 cut(s)	227, 626, 666, 1652, 1751
NspI	RCATGY	2 cut(s)	228, 1211
NspV	TTCGAA	2 cut(s)	1167, 1588
PaeI	GCATGC	1 cut(s)	1211
PagI	TCATGA	1 cut(s)	79
PceI	AGGCCT	1 cut(s)	169
PciSI	GCTCTTC	1 cut(s)	1590
PctI	GAATGC	1 cut(s)	1163
PflMI	CCANNNNNTGG	1 cut(s)	1916
PinAI	ACCGGT	1 cut(s)	1993
PkrI	GCNGC	4 cut(s)	612, 1132, 1405, 1876
PleI	GAGTC	4 cut(s)	154, 808, 1064, 1649
PpsI	GAGTC	4 cut(s)	154, 808, 1064, 1649
Ppu21I	YACGTR	1 cut(s)	198
PpuMI	RGGWCCY	1 cut(s)	954
PshAI	GACNNNNGTC	1 cut(s)	1552
PsiI	TTATAA	1 cut(s)	330
Psp124BI	GAGCTC	1 cut(s)	1585
Psp5II	RGGWCCY	1 cut(s)	954
Psp6I	CCWGG	1 cut(s)	1075
PspFI	CCCAGC	1 cut(s)	1682
PspGI	CCWGG	1 cut(s)	1075
PspN4I	GGNNCC	3 cut(s)	955, 1436, 1938
PspPI	GGNCC	4 cut(s)	342, 954, 1442, 2037
PspPPI	RGGWCCY	1 cut(s)	954
PstNI	CAGNNNCTG	2 cut(s)	1025, 1634
RsaI	GTAC	6 cut(s)	62, 110, 988, 1280, 1436, 1638
RsaNI	GTAC	6 cut(s)	61, 109, 987, 1279, 1435, 1637
RseI	CAYNNNNRTG	2 cut(s)	84, 921
SacI	GAGCTC	1 cut(s)	1585
SapI	GCTCTTC	1 cut(s)	1590
SaqAI	TTAA	3 cut(s)	1257, 1829, 2045
SatI	GCNGC	4 cut(s)	611, 1131, 1404, 1875
Sau3AI	GATC	6 cut(s)	76, 713, 1237, 1288, 1302, 1906
Sau96I	GGNCC	4 cut(s)	342, 954, 1442, 2037
ScaI	AGTACT	1 cut(s)	1280
SchI	GAGTC	4 cut(s)	155, 808, 1064, 1649
ScrFI	CCNGG	2 cut(s)	1077, 2086
SduI	GDGCHC	6 cut(s)	436, 647, 1068, 1585, 1749, 2029
SfaNI	GCATC	7 cut(s)	55, 697, 774, 1117, 1138, 1447, 1500
SfcI	CTRYAG	1 cut(s)	801
SfuI	TTCGAA	2 cut(s)	1167, 1588
SinI	GGWCC	3 cut(s)	342, 954, 1442
SmiMI	CAYNNNNRTG	2 cut(s)	84, 921
SphI	GCATGC	1 cut(s)	1211
SseBI	AGGCCT	1 cut(s)	169
SsiI	CCGC	2 cut(s)	761, 1967
SspMI	CTAG	1 cut(s)	1758
SstI	GAGCTC	1 cut(s)	1585
StuI	AGGCCT	1 cut(s)	169
StyD4I	CCNGG	2 cut(s)	1075, 2084
StyI	CCWWGG	4 cut(s)	544, 1284, 1380, 2029
TaaI	ACNGT	5 cut(s)	532, 632, 1021, 1439, 1636
TaiI	ACGT	5 cut(s)	200, 221, 324, 628, 1654
TaqI	TCGA	5 cut(s)	712, 1068, 1167, 1291, 1588
TatI	WGTACW	1 cut(s)	1278
Tru1I	TTAA	3 cut(s)	1257, 1829, 2045
Tru9I	TTAA	3 cut(s)	1257, 1829, 2045
TscAI	CASTG	4 cut(s)	637, 688, 816, 1026
TseFI	GTSAC	5 cut(s)	227, 626, 666, 1652, 1751
TseI	GCWGC	4 cut(s)	610, 1130, 1403, 1874
Tsp45I	GTSAC	5 cut(s)	227, 626, 666, 1652, 1751
TspDTI	ATGAA	6 cut(s)	87, 222, 453, 588, 1884, 1976
TspGWI	ACGGA	4 cut(s)	54, 126, 1319, 1409
TspRI	CASTG	4 cut(s)	637, 688, 816, 1026
Van91I	CCANNNNNTGG	1 cut(s)	1916
VneI	GTGCAC	1 cut(s)	432
VpaK11BI	GGWCC	3 cut(s)	342, 954, 1442
XagI	CCTNNNNNAGG	1 cut(s)	2044
XapI	RAATTY	4 cut(s)	510, 584, 1393, 1847
XceI	RCATGY	2 cut(s)	228, 1211
XcmI	CCANNNNNNNNNTGG	1 cut(s)	551
XspI	CTAG	1 cut(s)	1758
ZrmI	AGTACT	1 cut(s)	1280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.