MD08G1136300.v1.1

psbP domain-containing protein 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008432
Physical Location & Seq
Reverse (-)
12971418 .. 12974161
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1136300.v1.1.491

Sequence Viewer

Length: 873 bp
ATGGTTCTTCTCTCTCCTTCTCTCTCCCTCTTCCCTTCCAACCATGTTCTTCCTCCAAATATCCTTCCCAGAGAGCAAGGTAGGATTGTGATGCAGCAGCTGAACAAATGGCAAATAAGTTCAAGTGGGAACTCAAAACCCAGTTTACAAGATGGCTTTTGCAGGAGAGAGTTACTGCTGTTTGGCATTTCTGCTCCAGTTTTACTAGTCTTACCAGCTTCAGGGTCCCTTGCAGAGGAAGACTTGAAAATGGCTTCATTTGTTGATGACATAAATTCATATTCTTATCTATACCCTACGGAGTTGCCATCCAAGAAGGCCCTTTTCAAGTGGGTGGAATCGAGAAAGCCAGAGCGTTATTCATCAGCTGCACCATTATCTCCTGATGCGCGCCTGCGCATTGTTTCTGAGCGTGTTGACATTGTTGATAATCTCATCATTTCTATTTCGATAGGTCCCCCGAATTCAAAGATTATAAAATCACAAGACAAGAGTACATGGACTGCGAAAGATGTTGCTGATTCTGTTTTGGCTGACAAGTCTGCATTGCGTGTCACCTCAACTCAGCGGCAGGCTGAGAGTCAAGTCCTGGATGCACACACTGGTGAAATTGATGGTCAGCCATACTGGTACTATGAGTACCTTGTTCGCAAAGCACCCACAAAAACTGCTGACGAATCTACCAATTATAGACATTATGTGGCCTCAACAACTGAACGAGATGGTTACCTGTACTCTATTAATGCTTCAACCCTCAATAAGCAGTGGAACATAGTGGGGCCTATCTTACAAAAAACAGTAGCTTCATTTCGCCTTCTCCCTCCCACAGAAAACTACGTTCCTCCATTCAAGGATCCGTGGAGATTCTGGTGA

Protein Analysis

291

Amino Acids

32.69

Weight (kDa)

8.76

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbP PF01789 84 - 271 4.1e-14 PsbP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 476
Acc16I TGCGCA 1 cut(s) 398
AccII CGCG 1 cut(s) 391
AciI CCGC 1 cut(s) 568
AclWI GGATC 2 cut(s) 848, 861
AcsI RAATTY 2 cut(s) 274, 463
AcuI CTGAAG 1 cut(s) 204
AfaI GTAC 4 cut(s) 496, 632, 641, 734
AfiI CCNNNNNNNGG 2 cut(s) 221, 235
AgsI TTSAA 6 cut(s) 123, 247, 328, 468, 750, 850
AhlI ACTAGT 1 cut(s) 205
AjnI CCWGG 1 cut(s) 588
AleI CACNNNNGTG 1 cut(s) 603
AluBI AGCT 4 cut(s) 100, 218, 368, 803
AluI AGCT 4 cut(s) 100, 218, 368, 803
AlwI GGATC 2 cut(s) 848, 861
AlwNI CAGNNNCTG 1 cut(s) 100
AoxI GGCC 3 cut(s) 318, 702, 779
ApeKI GCWGC 3 cut(s) 94, 97, 368
ApoI RAATTY 2 cut(s) 274, 463
AseI ATTAAT 1 cut(s) 741
AspLEI GCGC 3 cut(s) 391, 393, 399
AspS9I GGNCC 4 cut(s) 225, 319, 455, 779
AsuHPI GGTGA 2 cut(s) 547, 617
AvaII GGWCC 2 cut(s) 225, 455
BamHI GGATCC 1 cut(s) 853
BbsI GAAGAC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 106, 109, 355
BccI CCATC 4 cut(s) 146, 316, 608, 716
BciT130I CCWGG 1 cut(s) 590
BcuI ACTAGT 1 cut(s) 205
BfaI CTAG 1 cut(s) 206
BisI GCNGC 4 cut(s) 95, 98, 369, 569
BlsI GCNGC 4 cut(s) 96, 99, 370, 570
Bme1390I CCNGG 1 cut(s) 590
Bme18I GGWCC 2 cut(s) 225, 455
BmgT120I GGNCC 4 cut(s) 225, 319, 455, 779
BmiI GGNNCC 5 cut(s) 226, 227, 457, 780, 855
BmrFI CCNGG 1 cut(s) 590
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 3 cut(s) 81, 376, 583
BmuI ACTGGG 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 246
BpmI CTGGAG 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 857
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 235
Bse1I ACTGG 4 cut(s) 141, 197, 607, 632
Bse3DI GCAATG 1 cut(s) 545
BseBI CCWGG 1 cut(s) 590
BseDI CCNNGG 1 cut(s) 857
BseGI GGATG 2 cut(s) 308, 598
BseLI CCNNNNNNNGG 2 cut(s) 221, 235
BseMI GCAATG 1 cut(s) 545
BseMII CTCAG 3 cut(s) 399, 567, 578
BseNI ACTGG 4 cut(s) 141, 197, 607, 632
BsePI GCGCGC 1 cut(s) 389
BseXI GCAGC 3 cut(s) 106, 109, 355
BsgI GTGCAG 1 cut(s) 354
Bsh1236I CGCG 1 cut(s) 391
BshFI GGCC 3 cut(s) 320, 704, 781
BslFI GGGAC 2 cut(s) 211, 441
BslI CCNNNNNNNGG 2 cut(s) 221, 235
BsmFI GGGAC 2 cut(s) 211, 441
BsnI GGCC 3 cut(s) 320, 704, 781
Bsp143I GATC 1 cut(s) 853
BspACI CCGC 1 cut(s) 568
BspANI GGCC 3 cut(s) 320, 704, 781
BspCNI CTCAG 3 cut(s) 400, 568, 577
BspFNI CGCG 1 cut(s) 391
BspLI GGNNCC 5 cut(s) 226, 227, 457, 780, 855
BspPI GGATC 2 cut(s) 848, 861
BsrDI GCAATG 1 cut(s) 545
BsrI ACTGG 4 cut(s) 141, 197, 607, 632
BssECI CCNNGG 1 cut(s) 857
BssHII GCGCGC 1 cut(s) 389
BssMI GATC 1 cut(s) 853
Bst2UI CCWGG 1 cut(s) 590
Bst4CI ACNGT 1 cut(s) 799
Bst6I CTCTTC 1 cut(s) 35
BstC8I GCNNGC 3 cut(s) 391, 395, 573
BstDEI CTNAG 3 cut(s) 408, 564, 576
BstDSI CCRYGG 1 cut(s) 857
BstEII GGTNACC 1 cut(s) 725
BstENI CCTNNNNNAGG 1 cut(s) 233
BstF5I GGATG 2 cut(s) 308, 598
BstFNI CGCG 1 cut(s) 391
BstHHI GCGC 3 cut(s) 391, 393, 399
BstKTI GATC 1 cut(s) 856
BstMBI GATC 1 cut(s) 853
BstNI CCWGG 1 cut(s) 590
BstPI GGTNACC 1 cut(s) 725
BstSCI CCNGG 1 cut(s) 588
BstUI CGCG 1 cut(s) 391
BstV1I GCAGC 3 cut(s) 106, 109, 355
BstV2I GAAGAC 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
BsuRI GGCC 3 cut(s) 320, 704, 781
BtgI CCRYGG 1 cut(s) 857
BtsCI GGATG 2 cut(s) 308, 598
BtsI GCAGTG 1 cut(s) 770
BtsIMutI CAGTG 2 cut(s) 600, 770
Cac8I GCNNGC 3 cut(s) 391, 395, 573
CaiI CAGNNNCTG 1 cut(s) 100
CfoI GCGC 3 cut(s) 391, 393, 399
Cfr13I GGNCC 4 cut(s) 225, 319, 455, 779
Csp6I GTAC 4 cut(s) 495, 631, 640, 733
CviAII CATG 2 cut(s) 44, 498
CviQI GTAC 4 cut(s) 495, 631, 640, 733
DdeI CTNAG 3 cut(s) 408, 564, 576
DpnI GATC 1 cut(s) 855
DpnII GATC 1 cut(s) 853
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco47I GGWCC 2 cut(s) 225, 455
Eco57I CTGAAG 1 cut(s) 204
Eco91I GGTNACC 1 cut(s) 725
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoO109I RGGNCCY 4 cut(s) 225, 319, 455, 779
EcoO65I GGTNACC 1 cut(s) 725
EcoRI GAATTC 1 cut(s) 463
EcoRII CCWGG 1 cut(s) 588
FaeI CATG 2 cut(s) 47, 501
FaqI GGGAC 2 cut(s) 211, 441
FatI CATG 2 cut(s) 43, 497
Fnu4HI GCNGC 4 cut(s) 95, 98, 369, 569
FokI GGATG 2 cut(s) 295, 605
Fsp4HI GCNGC 4 cut(s) 95, 98, 369, 569
FspBI CTAG 1 cut(s) 206
FspI TGCGCA 1 cut(s) 398
GlaI GCGC 3 cut(s) 390, 392, 398
GluI GCNGC 4 cut(s) 95, 98, 369, 569
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 3 cut(s) 320, 704, 781
HhaI GCGC 3 cut(s) 391, 393, 399
Hin1II CATG 2 cut(s) 47, 501
Hin6I GCGC 3 cut(s) 389, 391, 397
HinP1I GCGC 3 cut(s) 389, 391, 397
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 5 cut(s) 338, 521, 580, 677, 864
HphI GGTGA 2 cut(s) 547, 617
Hpy166II GTNNAC 2 cut(s) 146, 418
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 2 cut(s) 342, 383
Hpy8I GTNNAC 2 cut(s) 146, 418
HpyAV CCTTC 5 cut(s) 27, 45, 74, 310, 824
HpyCH4III ACNGT 1 cut(s) 799
HpyCH4IV ACGT 1 cut(s) 837
HpyCH4V TGCA 6 cut(s) 94, 162, 233, 371, 545, 596
HpyF3I CTNAG 3 cut(s) 408, 564, 576
HpySE526I ACGT 1 cut(s) 837
Hsp92II CATG 2 cut(s) 47, 501
HspAI GCGC 3 cut(s) 389, 391, 397
KflI GGGWCCC 1 cut(s) 225
Kzo9I GATC 1 cut(s) 853
LmnI GCTCC 1 cut(s) 199
Lsp1109I GCAGC 3 cut(s) 106, 109, 355
LweI GCATC 3 cut(s) 81, 376, 583
MaeI CTAG 1 cut(s) 206
MaeII ACGT 1 cut(s) 837
MaeIII GTNAC 3 cut(s) 171, 553, 725
MalI GATC 1 cut(s) 855
MboI GATC 1 cut(s) 853
MboII GAAGA 3 cut(s) 22, 41, 251
MflI RGATCY 1 cut(s) 853
MluCI AATT 4 cut(s) 274, 463, 609, 685
MlyI GAGTC 1 cut(s) 589
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 8 cut(s) 38, 63, 229, 568, 715, 764, 831, 852
MseI TTAA 1 cut(s) 741
MslI CAYNNNNRTG 1 cut(s) 603
MspA1I CMGCKG 3 cut(s) 100, 368, 568
MspR9I CCNGG 1 cut(s) 590
MvaI CCWGG 1 cut(s) 590
MvnI CGCG 1 cut(s) 391
NdeII GATC 1 cut(s) 853
NlaIII CATG 2 cut(s) 47, 501
NlaIV GGNNCC 5 cut(s) 226, 227, 457, 780, 855
NmuCI GTSAC 1 cut(s) 553
NsbI TGCGCA 1 cut(s) 398
OliI CACNNNNGTG 1 cut(s) 603
PauI GCGCGC 1 cut(s) 389
PfeI GAWTC 4 cut(s) 338, 521, 677, 864
PfoI TCCNGGA 1 cut(s) 588
PkrI GCNGC 4 cut(s) 96, 99, 370, 570
PleI GAGTC 1 cut(s) 588
PpsI GAGTC 1 cut(s) 588
PpuMI RGGWCCY 2 cut(s) 225, 455
PshBI ATTAAT 1 cut(s) 741
PsiI TTATAA 1 cut(s) 476
Psp5II RGGWCCY 2 cut(s) 225, 455
Psp6I CCWGG 1 cut(s) 588
PspEI GGTNACC 1 cut(s) 725
PspGI CCWGG 1 cut(s) 588
PspN4I GGNNCC 5 cut(s) 226, 227, 457, 780, 855
PspPI GGNCC 4 cut(s) 225, 319, 455, 779
PspPPI RGGWCCY 2 cut(s) 225, 455
PstNI CAGNNNCTG 1 cut(s) 100
PsuI RGATCY 1 cut(s) 853
PteI GCGCGC 1 cut(s) 389
PvuII CAGCTG 2 cut(s) 100, 368
RsaI GTAC 4 cut(s) 496, 632, 641, 734
RsaNI GTAC 4 cut(s) 495, 631, 640, 733
RseI CAYNNNNRTG 1 cut(s) 603
SaqAI TTAA 1 cut(s) 741
SatI GCNGC 4 cut(s) 95, 98, 369, 569
Sau3AI GATC 1 cut(s) 853
Sau96I GGNCC 4 cut(s) 225, 319, 455, 779
SchI GAGTC 1 cut(s) 589
ScrFI CCNGG 1 cut(s) 590
SfaNI GCATC 3 cut(s) 81, 376, 583
SinI GGWCC 2 cut(s) 225, 455
SmiMI CAYNNNNRTG 1 cut(s) 603
SpeI ACTAGT 1 cut(s) 205
Sse9I AATT 4 cut(s) 274, 463, 609, 685
SsiI CCGC 1 cut(s) 568
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 1 cut(s) 588
TaaI ACNGT 1 cut(s) 799
TaiI ACGT 1 cut(s) 840
TaqI TCGA 2 cut(s) 341, 449
TasI AATT 4 cut(s) 274, 463, 609, 685
TatI WGTACW 2 cut(s) 494, 732
TauI GCSGC 1 cut(s) 571
TfiI GAWTC 4 cut(s) 338, 521, 677, 864
Tru1I TTAA 1 cut(s) 741
Tru9I TTAA 1 cut(s) 741
TscAI CASTG 2 cut(s) 607, 770
TseFI GTSAC 1 cut(s) 553
TseI GCWGC 3 cut(s) 94, 97, 368
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 4 cut(s) 246, 267, 351, 795
TspGWI ACGGA 2 cut(s) 314, 846
TspRI CASTG 2 cut(s) 607, 770
VpaK11BI GGWCC 2 cut(s) 225, 455
VspI ATTAAT 1 cut(s) 741
XagI CCTNNNNNAGG 1 cut(s) 233
XapI RAATTY 2 cut(s) 274, 463
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.