MD08G1149300.v1.1

40S ribosomal protein S30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003860
Physical Location & Seq
Forward (+)
15133691 .. 15133882
192 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1149300.v1.1.491

Sequence Viewer

Length: 192 bp
ATGGGTAAGGTTCACGGATCGCTCGCACGTGCCGGGAACGTTAGGGGTCAGACTCCAAAGGTGGCGAAGCAAGACAAGAAGAAGAAGCCCAGAGGCCGCGCCCACAAGAGGCAGCAGTACAACAGGAGATTCGTCACAGCCGTTGTTGGATTCGGAAGAAGACGGGGTACGTGCTGCACAGCCAGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S30

Protein Analysis

64

Amino Acids

7.05

Weight (kDa)

12.0

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S30 PF04758 3 - 57 4.2e-28 Ribosomal protein S30
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 99
AciI CCGC 1 cut(s) 97
AclI AACGTT 1 cut(s) 39
AclWI GGATC 1 cut(s) 25
AcvI CACGTG 1 cut(s) 29
AfaI GTAC 2 cut(s) 119, 169
AfiI CCNNNNNNNGG 1 cut(s) 108
AlwI GGATC 1 cut(s) 25
AoxI GGCC 1 cut(s) 94
ApeKI GCWGC 2 cut(s) 112, 174
AspLEI GCGC 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 34
BarI GAAGNNNNNNTAC 2 cut(s) 151, 183
BbrPI CACGTG 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 124, 161
BceAI ACGGC 1 cut(s) 125
BcnI CCSGG 1 cut(s) 34
BglII AGATCT 1 cut(s) 185
BisI GCNGC 3 cut(s) 97, 113, 175
BlsI GCNGC 3 cut(s) 98, 114, 176
Bme1390I CCNGG 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 34
BpiI GAAGAC 1 cut(s) 166
BpuMI CCSGG 1 cut(s) 34
BsaAI YACGTR 2 cut(s) 29, 171
Bsc4I CCNNNNNNNGG 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 108
BseXI GCAGC 2 cut(s) 124, 161
BsgI GTGCAG 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 96
BsiSI CCGG 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 108
BsnI GGCC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 17, 185
BspACI CCGC 1 cut(s) 97
BspANI GGCC 1 cut(s) 96
BspFNI CGCG 1 cut(s) 99
BspPI GGATC 1 cut(s) 25
BssMI GATC 2 cut(s) 17, 185
BstBAI YACGTR 2 cut(s) 29, 171
BstC8I GCNNGC 1 cut(s) 24
BstFNI CGCG 1 cut(s) 99
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 2 cut(s) 20, 188
BstMBI GATC 2 cut(s) 17, 185
BstSCI CCNGG 1 cut(s) 32
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 124, 161
BstV2I GAAGAC 1 cut(s) 166
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BsuRI GGCC 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 24
CfoI GCGC 1 cut(s) 101
Csp6I GTAC 2 cut(s) 118, 168
CviJI RGCY 4 cut(s) 88, 96, 140, 182
CviKI_1 RGCY 4 cut(s) 88, 96, 140, 182
CviQI GTAC 2 cut(s) 118, 168
DpnI GATC 2 cut(s) 19, 187
DpnII GATC 2 cut(s) 17, 185
Eco72I CACGTG 1 cut(s) 29
Fnu4HI GCNGC 3 cut(s) 97, 113, 175
Fsp4HI GCNGC 3 cut(s) 97, 113, 175
GlaI GCGC 1 cut(s) 100
GluI GCNGC 3 cut(s) 97, 113, 175
HaeIII GGCC 1 cut(s) 96
HapII CCGG 1 cut(s) 33
HhaI GCGC 1 cut(s) 101
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HinfI GANTC 3 cut(s) 52, 129, 150
HpaII CCGG 1 cut(s) 33
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 2 cut(s) 51, 155
Hpy188III TCNNGA 1 cut(s) 189
Hpy8I GTNNAC 1 cut(s) 13
HpyCH4IV ACGT 3 cut(s) 28, 39, 170
HpyCH4V TGCA 1 cut(s) 177
HpySE526I ACGT 3 cut(s) 28, 39, 170
HspAI GCGC 1 cut(s) 99
Kzo9I GATC 2 cut(s) 17, 185
LpnPI CCDG 3 cut(s) 46, 103, 109
Lsp1109I GCAGC 2 cut(s) 124, 161
MaeII ACGT 3 cut(s) 28, 39, 170
MaeIII GTNAC 1 cut(s) 133
MalI GATC 2 cut(s) 19, 187
MboI GATC 2 cut(s) 17, 185
MboII GAAGA 4 cut(s) 91, 94, 168, 171
MflI RGATCY 1 cut(s) 185
MlyI GAGTC 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 2 cut(s) 86, 102
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 34
MvnI CGCG 1 cut(s) 99
NciI CCSGG 1 cut(s) 34
NdeII GATC 2 cut(s) 17, 185
NmuCI GTSAC 1 cut(s) 133
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PfeI GAWTC 2 cut(s) 129, 150
PkrI GCNGC 3 cut(s) 98, 114, 176
PleI GAGTC 1 cut(s) 46
PmaCI CACGTG 1 cut(s) 29
PmlI CACGTG 1 cut(s) 29
PpsI GAGTC 1 cut(s) 46
Ppu21I YACGTR 2 cut(s) 29, 171
Psp1406I AACGTT 1 cut(s) 39
PspCI CACGTG 1 cut(s) 29
PsuI RGATCY 1 cut(s) 185
RsaI GTAC 2 cut(s) 119, 169
RsaNI GTAC 2 cut(s) 118, 168
SatI GCNGC 3 cut(s) 97, 113, 175
Sau3AI GATC 2 cut(s) 17, 185
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 1 cut(s) 34
SetI ASST 5 cut(s) 12, 31, 42, 63, 173
SsiI CCGC 1 cut(s) 97
StyD4I CCNGG 1 cut(s) 32
TaiI ACGT 3 cut(s) 31, 42, 173
TatI WGTACW 1 cut(s) 117
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 2 cut(s) 129, 150
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 2 cut(s) 112, 174
Tsp45I GTSAC 1 cut(s) 133
TspGWI ACGGA 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.