Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003067

Physical Location & Seq

Genomic Coordinates Reverse (-)

22953944 .. 22966102

Gene Structure 12159 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1184300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3300 bp

ATGGCCTTCCCGCCCGCCGACGACCACCACAACCCTAACCCTAACCACCTCCACCTTGAATCACTCCCTCTCATTGACCTCGGCCTCCTCTCTCAATCAGACCTCTACTCTCTCTCCCTCATCTCCTCCTCCTCCTCCCAATCCCACCCCACCCGCCGCTTCGACGATGACGTGTTGATCCCCAAAATCGACCGTTCCGTCTTCAATGAATCCGCCGGCAGCCGCAAGCAGACCTACTCCCGACTCCGCCTCGCTCCTCGTAACTCCCAATTCCCAATCCCAAACCCCAAATCGCAGAAAACGATTCCCCATTTCCACCCCCGAGACTCCGAAACCCTCCAGATTATCGCTCTCTTGAAACAGCTTTTTCCCTCAGAAAAAGCCCCAAACGAAAACGACGACGTACTCGTCCCTGTCCCCGTCCACCTCGCCCAATACGACGCCACGCCCGGCCCATTCGTGGAAAACTTCCCGGCTGGACTCTCCGTCGATATCAGTAAGAAGAGGAAGAGAGGCCGCCCGCGTAAGGATCATAATGCCATTATATCTTACCGGAGCGTCAACGCTGATGTTTCGATGAAGATGCGTGGTGGTGACAATACGCCGCCCGGGATTGTTGTTCAGAGCAGTGAGGGCAGCCGGAAGCGAGGCCGGCCGCGGAAAGATGAGAACAGAGTGGTTTCGGTGGCTAAGCGTGAGAAGAAAGTGACGGTCAAGGAAGGGAAAGTGAAGGTGGAGGAGGGTGAGATGGTGATGGTGAACGGAAATGGAGTTGTTGTGGACTTGGCTGCGGTGGAGAATGCGGACGGTTTGTTTGGGGAGGAGTTGAGAAGGAGGACGGAGGCGATGGAGACGCAGGCGCAGTTGTTAGGGTTTCTGGGAGGGTTGGAGGGGCAGTGGTCGAGCGCTAGGAAGAAGAGGAAGATTGTGCCTGCGGGTGAGCTTGGTGACGTGTTGCCGATGCATTGGAAGATTTTGCTATCTCTCAAGAGGAATGGAGGCCTAGTTCGACTCTTTTGTAAACGATACATAAGCCCTAATGGGCAGCAGTTTGTGTCATGCAAGGAAGTTAATTCGTACTTGATCTCTTATTTTGGAGTTGGAAACAAGTCAATTCCTGGTCATACAGGTGGCAGTATTCGGCTTTCTAATAAAATGGTTTCTGGAGATGATACAAATCTTACCTTTGAAGATGAGAGCAAAGCTAATGAGCTTGCTAGCCGCTCGCTAATGCCTGTTACTTCAGTATCACCTGGTCATGAGAAACAAGAAAACTTCTTTCATACAGAAAATTTACAGGAATTTCACGCAGGAGAAGAAATGGGCATGCCTAAGAAAGTTGACCCGATAAAGACTGGTATTCTGCAGACATCTTTGAAGTTTCATGAGTCGGGTCAAATAGACAGGGAAGCATCTTCTGATGCAGTAGCCAAATATGAATCGAGTGCTGGTTTTCCTTGTGGTAAAGTGAATAATGATATCACAAATTGTTTTGGATCGAACAATAGTGAATCTAATTCATTGTCTTGTGAGGATCAAGCTTTGAAGAATAATAAAATTGGTAAGGATTTGGGATTTTCTGAGCAGGGTAGTGCAGTTATTATGACAAATGATAAGTTGGTGGATGAGCCTACTGGTCCTGGTCTCAGCTTTGGTGAAACAAATATTTTGGACTGCTTTCATGGTGGAATAAATGATGGTTTTAAGGCTTCTGTGAGTTCAGTTGATTTGCCCAAAGTAGACACTGATACTGCTTTAAATGGTACGCGAAAAGAACGGAGGTCTGAGAATATTGATAACAACCAATTAACTAGCACAGAAGAAAGTACAAAGTTTGATGATGTGGATGATCGTGGGAATGGCAGGTTGATATCTGGTATTAGTGAGAATTGCATTGGACCCCGTAATGTTTGTACTAATGTCGTGCAGCAAAGTACTTCTGAAGCCTGCTCGCTTCTTCCTTCAACTATCATACAGTCTCTGCAGAACAGAGGCTCTGAAAGAGGTTTATGTAGTACTACTGTTGAGAAAACATGTGATGTCAGGGATGATTTCAATAGTGAGTCACTCTGCACATCTTATGGGCCCAAGTTTGATAATATTGATCTATCTGGGAATGACGACAAGGCAATCAGTTTAAGCAGCGACCATGCTATGCCGAATGCGGGTGCAACGGAAATCAATGAGCATGTTATAAGTACTGGGAGCTGTTCAAGCGTCCCAGGTTTGGATGGACAAAGTTGTATCGCATTAAGTAATGTAAAGAATCCTAGTTGCATGGTGGAGGAACTCTGGCAGGAAAAGAGTTTTCAAAGCAATGTGTCTGTTCCATTTGTTAAACAGGGCAGGGGTTCTTCTGGAACCGGTATGATTAACCCCTCTGGCTATGCATGCAATGTGAATAGCACATACTCAAGCTCAATGGAAGAATCAAAACATGAGGATTCTAAAAGGTCTTGGAATAAGGAGGTAATGCTTTCTTTTAGCAACAGTTCTGCTGGATCAGTTGCCAATTCTTTGACAAAGACTCCGCATGAAAGGAGTTCTGGAGGACCTTCCCTTGTTCATCCAGGGAACAGTCAAACATTTTTCACTGGAAATAAATTAACTGGCATTTTCAGTAGCTCAGTATTGGATGATAAGCTCATTAGAGGTTCTGTTGATGGTTCAATACATCCAAATGGTGAAGAACAAATCCCTGGGATAGAACATAATATAAACAGGGTTTATGGCAGCATAATGTGGGAGCAGCCTAACACTGAGAGTGCTGAAAAGTCCAGGAACAATGAGCCCCTAAATCATTCCCAACCTTCTGGGGGTGTCATGGCTGAATTTATGTGGAGAAGTGATGGGCAAAATGTTCAGCAGAGTGGACTGGTTGATACACCATTTGAACTCATGCAATCATCTGGCCACTATCCGAACATTGGTCTGTTGTCTGACAAGGGTGCAAACGGAGCTTTAAGTGTAAATGAAAAGCGTGACAACATGTCAGGTTTGGAAGGATTGAAACTGGGTCAGTTTGATTATAATTTCCCAACAGTGCAAGCAACCTCACATACAAAGGAATCAAAAGTGTTGTCATATGATGGAGAGATGGAACAAGGTTTCAGTTCATCAGATTGGCTTGAAAAGGAATCTTTGCCCTCAATGCCAAATATCGCAAGCGGGTATGAGATTAACACCTGTGTCTGGTGCAGAAATGAGTTTCATCATGAGGTTTGTGAACGGACACAACCTGGTTCTGTCGGTTTGATGTGTGCAACCTGCCAGGCCAATTTCTCAGGCAATTTTTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0008327 GO:0036094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AT_hook MBD

Protein Analysis

1100

Amino Acids

119.84

Weight (kDa)

5.65

Isoelectric Point (pI)

48.81

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003067)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120
malus_domestica	MD00G1009700.v1.1 MD08G1184300.v1.1 MD15G1370800.v1.1
prunus_persica	Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1
pyrus_communis	pycom08g15800
rosa_chinensis	RchiOBHm_Chr7g0238671 RchiOBHm_Chr7g0243951 RchiOBHm_Chr7g0243961
rosa_laevigata	RLG00000000820
rosa_multiflora	Rmu_sc0006110.1_g000008
rosa_roxburghii	Rroxscaffold_3G00221630
rosa_rugosa	Rorug07G0322800
rosa_samantha	Rh7AG477200 Rh7BG448500 Rh7CG495300 Rh7DG462200
rosa_wichuraiana	Rw7G039830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2195, 3030
AasI	GACNNNNNNGTC	2 cut(s)	197, 407
Acc36I	ACCTGC	2 cut(s)	1854, 3275
AccBSI	CCGCTC	1 cut(s)	1224
AccI	GTMKAC	1 cut(s)	1740
AccII	CGCG	3 cut(s)	523, 658, 1768
AclWI	GGATC	5 cut(s)	172, 537, 1504, 1542, 2509
AcoI	YGGCCR	2 cut(s)	653, 2911
AcsI	RAATTY	3 cut(s)	1291, 1301, 2831
AcuI	CTGAAG	2 cut(s)	1227, 1962
AcyI	GRCGYC	1 cut(s)	441
AfaI	GTAC	8 cut(s)	405, 1079, 1765, 1828, 1915, 1936, 2017, 2200
AfeI	AGCGCT	1 cut(s)	907
AfiI	CCNNNNNNNGG	7 cut(s)	460, 526, 2165, 2227, 2816, 2927, 3192
AflIII	ACRYGT	4 cut(s)	171, 951, 2033, 2988
AgeI	ACCGGT	1 cut(s)	2363
AhdI	GACNNNNNGTC	2 cut(s)	485, 2989
AjiI	CACGTC	2 cut(s)	172, 952
AjnI	CCWGG	9 cut(s)	1117, 1252, 1639, 2221, 2569, 2698, 2777, 3238, 3270
AloI	GAACNNNNNNTCC	2 cut(s)	990, 1022
Alw26I	GTCTC	4 cut(s)	318, 845, 1649, 1983
AlwI	GGATC	5 cut(s)	172, 537, 1504, 1542, 2509
AlwNI	CAGNNNCTG	1 cut(s)	1981
Ama87I	CYCGRG	2 cut(s)	321, 608
Aor51HI	AGCGCT	1 cut(s)	907
ApaI	GGGCCC	1 cut(s)	2088
ApeKI	GCWGC	8 cut(s)	219, 636, 788, 1045, 1927, 2142, 2733, 2749
ApoI	RAATTY	3 cut(s)	1291, 1301, 2831
ArsI	GACNNNNNNTTYG	4 cut(s)	797, 829, 3065, 3097
AsiGI	ACCGGT	1 cut(s)	2363
AspLEI	GCGC	2 cut(s)	862, 908
AspS9I	GGNCC	6 cut(s)	452, 1637, 1898, 2084, 2085, 2552
AsuC2I	CCSGG	4 cut(s)	450, 473, 609, 610
AsuHPI	GGTGA	9 cut(s)	605, 755, 763, 769, 950, 959, 1242, 1667, 2696
AsuNHI	GCTAGC	1 cut(s)	1217
AvaI	CYCGRG	2 cut(s)	321, 608
AvaII	GGWCC	3 cut(s)	1637, 1898, 2552
BaeGI	GKGCMC	1 cut(s)	2088
BaeI	ACNNNNGTAYC	2 cut(s)	1230, 1263
BalI	TGGCCA	1 cut(s)	2913
BanII	GRGCYC	2 cut(s)	2088, 2793
BbsI	GAAGAC	1 cut(s)	193
BbvI	GCAGC	8 cut(s)	231, 648, 775, 1057, 1939, 2154, 2745, 2761
BccI	CCATC	9 cut(s)	742, 748, 841, 1691, 2225, 2657, 2843, 3083, 3091
BcgI	CGANNNNNNTGC	2 cut(s)	565, 599
BciT130I	CCWGG	9 cut(s)	1119, 1254, 1641, 2223, 2571, 2700, 2779, 3240, 3272
BcnI	CCSGG	4 cut(s)	450, 473, 609, 610
BcoDI	GTCTC	4 cut(s)	318, 845, 1649, 1983
BfaI	CTAG	5 cut(s)	909, 1004, 1218, 1812, 2271
BfmI	CTRYAG	2 cut(s)	1364, 1982
BfoI	RGCGCY	1 cut(s)	909
BfuAI	ACCTGC	2 cut(s)	1854, 3275
BlpI	GCTNAGC	1 cut(s)	690
BmcAI	AGTACT	3 cut(s)	1936, 2017, 2200
Bme18I	GGWCC	3 cut(s)	1637, 1898, 2552
BmeRI	GACNNNNNGTC	2 cut(s)	485, 2989
BmeT110I	CYCGRG	2 cut(s)	321, 608
BmgBI	CACGTC	2 cut(s)	172, 952
BmgT120I	GGNCC	6 cut(s)	452, 1637, 1898, 2084, 2085, 2552
BmiI	GGNNCC	3 cut(s)	1900, 2086, 2362
BmrI	ACTGGG	2 cut(s)	2211, 3023
BmsI	GCATC	4 cut(s)	573, 951, 1411, 1421
BmtI	GCTAGC	1 cut(s)	1221
BmuI	ACTGGG	2 cut(s)	2211, 3023
BpiI	GAAGAC	1 cut(s)	193
BpmI	CTGGAG	3 cut(s)	323, 1185, 2568
Bpu1102I	GCTNAGC	1 cut(s)	690
BpuEI	CTTGAG	2 cut(s)	971, 2398
BpuMI	CCSGG	4 cut(s)	450, 473, 609, 610
BsaBI	GATNNNNATC	1 cut(s)	1176
BsaHI	GRCGYC	1 cut(s)	441
BsaI	GGTCTC	1 cut(s)	1649
BsaJI	CCNNGG	7 cut(s)	79, 608, 656, 2221, 2570, 2698, 2699
BsaWI	WCCGGW	2 cut(s)	552, 2363
Bsc4I	CCNNNNNNNGG	7 cut(s)	460, 526, 2165, 2227, 2816, 2927, 3192
Bse118I	RCCGGY	3 cut(s)	215, 651, 2363
Bse1I	ACTGG	7 cut(s)	1360, 1639, 2206, 2599, 2614, 2880, 3018
Bse3DI	GCAATG	2 cut(s)	2323, 2401
Bse8I	GATNNNNATC	1 cut(s)	1176
BseBI	CCWGG	9 cut(s)	1119, 1254, 1641, 2223, 2571, 2700, 2779, 3240, 3272
BseDI	CCNNGG	7 cut(s)	79, 608, 656, 2221, 2570, 2698, 2699
BseGI	GGATG	7 cut(s)	1630, 1852, 2053, 2236, 2566, 2641, 2674
BseJI	GATNNNNATC	1 cut(s)	1176
BseLI	CCNNNNNNNGG	7 cut(s)	460, 526, 2165, 2227, 2816, 2927, 3192
BseMI	GCAATG	2 cut(s)	2323, 2401
BseMII	CTCAG	7 cut(s)	387, 1572, 1660, 1776, 2640, 2751, 3297
BseNI	ACTGG	7 cut(s)	1360, 1639, 2206, 2599, 2614, 2880, 3018
BseRI	GAGGAG	8 cut(s)	77, 115, 118, 121, 124, 246, 752, 836
BseSI	GKGCMC	1 cut(s)	2088
BseX3I	CGGCCG	1 cut(s)	653
BseXI	GCAGC	8 cut(s)	231, 648, 775, 1057, 1939, 2154, 2745, 2761
BsgI	GTGCAG	4 cut(s)	1614, 1946, 2056, 3217
Bsh1236I	CGCG	3 cut(s)	523, 658, 1768
Bsh1285I	CGRYCG	2 cut(s)	193, 656
BshTI	ACCGGT	1 cut(s)	2363
BsiEI	CGRYCG	2 cut(s)	193, 656
BsiHKCI	CYCGRG	2 cut(s)	321, 608
BsiSI	CCGG	8 cut(s)	216, 450, 473, 553, 609, 640, 652, 2364
BslFI	GGGAC	3 cut(s)	395, 401, 2204
BslI	CCNNNNNNNGG	7 cut(s)	460, 526, 2165, 2227, 2816, 2927, 3192
BsmAI	GTCTC	4 cut(s)	318, 845, 1649, 1983
BsmBI	CGTCTC	1 cut(s)	845
BsmFI	GGGAC	3 cut(s)	395, 401, 2204
BsmI	GAATGC	2 cut(s)	805, 2167
Bso31I	GGTCTC	1 cut(s)	1649
BsoBI	CYCGRG	2 cut(s)	321, 608
Bsp120I	GGGCCC	1 cut(s)	2084
Bsp1286I	GDGCHC	2 cut(s)	2088, 2793
Bsp143I	GATC	8 cut(s)	177, 529, 1083, 1496, 1534, 1849, 2104, 2501
Bsp1720I	GCTNAGC	1 cut(s)	690
BspCNI	CTCAG	7 cut(s)	386, 1573, 1659, 1777, 2639, 2752, 3296
BspFNI	CGCG	3 cut(s)	523, 658, 1768
BspHI	TCATGA	3 cut(s)	1258, 1384, 3214
BspLI	GGNNCC	3 cut(s)	1900, 2086, 2362
BspMAI	CTGCAG	2 cut(s)	1368, 1986
BspMI	ACCTGC	2 cut(s)	1854, 3275
BspOI	GCTAGC	1 cut(s)	1221
BspPI	GGATC	5 cut(s)	172, 537, 1504, 1542, 2509
BspTNI	GGTCTC	1 cut(s)	1649
BsrBI	CCGCTC	1 cut(s)	1224
BsrDI	GCAATG	2 cut(s)	2323, 2401
BsrFI	RCCGGY	3 cut(s)	215, 651, 2363
BsrI	ACTGG	7 cut(s)	1360, 1639, 2206, 2599, 2614, 2880, 3018
BssAI	RCCGGY	3 cut(s)	215, 651, 2363
BssECI	CCNNGG	7 cut(s)	79, 608, 656, 2221, 2570, 2698, 2699
BssMI	GATC	8 cut(s)	177, 529, 1083, 1496, 1534, 1849, 2104, 2501
BssNI	GRCGYC	1 cut(s)	441
Bst2UI	CCWGG	9 cut(s)	1119, 1254, 1641, 2223, 2571, 2700, 2779, 3240, 3272
Bst4CI	ACNGT	8 cut(s)	194, 712, 809, 1977, 2023, 2492, 2579, 3043
Bst6I	CTCTTC	3 cut(s)	497, 503, 911
BstACI	GRCGYC	1 cut(s)	441
BstDEI	CTNAG	9 cut(s)	373, 690, 1332, 1581, 1646, 1785, 2626, 2760, 3283
BstDSI	CCRYGG	1 cut(s)	656
BstF5I	GGATG	7 cut(s)	1630, 1852, 2053, 2236, 2566, 2641, 2674
BstFNI	CGCG	3 cut(s)	523, 658, 1768
BstH2I	RGCGCY	1 cut(s)	909
BstHHI	GCGC	2 cut(s)	862, 908
BstKTI	GATC	8 cut(s)	180, 532, 1086, 1499, 1537, 1852, 2107, 2504
BstMAI	GTCTC	4 cut(s)	318, 845, 1649, 1983
BstMBI	GATC	8 cut(s)	177, 529, 1083, 1496, 1534, 1849, 2104, 2501
BstMCI	CGRYCG	2 cut(s)	193, 656
BstMWI	GCNNNNNNNGC	6 cut(s)	633, 652, 2214, 2391, 2957, 3151
BstNI	CCWGG	9 cut(s)	1119, 1254, 1641, 2223, 2571, 2700, 2779, 3240, 3272
BstNSI	RCATGY	5 cut(s)	1330, 2037, 2192, 2394, 2992
BstSFI	CTRYAG	2 cut(s)	1364, 1982
BstSLI	GKGCMC	1 cut(s)	2088
BstUI	CGCG	3 cut(s)	523, 658, 1768
BstV1I	GCAGC	8 cut(s)	231, 648, 775, 1057, 1939, 2154, 2745, 2761
BstV2I	GAAGAC	1 cut(s)	193
BstXI	CCANNNNNNTGG	1 cut(s)	2813
BstZI	CGGCCG	1 cut(s)	653
BtgI	CCRYGG	1 cut(s)	656
BtgZI	GCGATG	1 cut(s)	860
BtrI	CACGTC	2 cut(s)	172, 952
BtsCI	GGATG	7 cut(s)	1630, 1852, 2053, 2236, 2566, 2641, 2674
BtsI	GCAGTG	2 cut(s)	634, 902
BtsIMutI	CAGTG	6 cut(s)	634, 902, 1743, 2592, 2757, 3048
BveI	ACCTGC	2 cut(s)	1854, 3275
CaiI	CAGNNNCTG	1 cut(s)	1981
CciI	TCATGA	3 cut(s)	1258, 1384, 3214
CfoI	GCGC	2 cut(s)	862, 908
Cfr10I	RCCGGY	3 cut(s)	215, 651, 2363
Cfr13I	GGNCC	6 cut(s)	452, 1637, 1898, 2084, 2085, 2552
Cfr42I	CCGCGG	1 cut(s)	659
Cfr9I	CCCGGG	1 cut(s)	608
CseI	GACGC	4 cut(s)	449, 547, 862, 2206
CsiI	ACCWGGT	2 cut(s)	1252, 3238
Csp6I	GTAC	8 cut(s)	404, 1078, 1764, 1827, 1914, 1935, 2016, 2199
CspAI	ACCGGT	1 cut(s)	2363
CviQI	GTAC	8 cut(s)	404, 1078, 1764, 1827, 1914, 1935, 2016, 2199
DdeI	CTNAG	9 cut(s)	373, 690, 1332, 1581, 1646, 1785, 2626, 2760, 3283
DpnI	GATC	8 cut(s)	179, 531, 1085, 1498, 1536, 1851, 2106, 2503
DpnII	GATC	8 cut(s)	177, 529, 1083, 1496, 1534, 1849, 2104, 2501
DraI	TTTAAA	1 cut(s)	1758
DrdI	GACNNNNNNGTC	2 cut(s)	197, 407
DriI	GACNNNNNGTC	2 cut(s)	485, 2989
DseDI	GACNNNNNNGTC	2 cut(s)	197, 407
EaeI	YGGCCR	2 cut(s)	653, 2911
EagI	CGGCCG	1 cut(s)	653
Eam1104I	CTCTTC	3 cut(s)	497, 503, 911
Eam1105I	GACNNNNNGTC	2 cut(s)	485, 2989
EarI	CTCTTC	3 cut(s)	497, 503, 911
EciI	GGCGGA	2 cut(s)	202, 236
EclXI	CGGCCG	1 cut(s)	653
Eco147I	AGGCCT	1 cut(s)	1002
Eco24I	GRGCYC	2 cut(s)	2088, 2793
Eco31I	GGTCTC	1 cut(s)	1649
Eco32I	GATATC	3 cut(s)	493, 1480, 1872
Eco47I	GGWCC	3 cut(s)	1637, 1898, 2552
Eco47III	AGCGCT	1 cut(s)	907
Eco52I	CGGCCG	1 cut(s)	653
Eco57I	CTGAAG	2 cut(s)	1227, 1962
Eco88I	CYCGRG	2 cut(s)	321, 608
EcoO109I	RGGNCCY	1 cut(s)	2552
EcoRII	CCWGG	9 cut(s)	1117, 1252, 1639, 2221, 2569, 2698, 2777, 3238, 3270
EcoRV	GATATC	3 cut(s)	493, 1480, 1872
EcoT22I	ATGCAT	2 cut(s)	966, 2392
EcoT38I	GRGCYC	2 cut(s)	2088, 2793
Esp3I	CGTCTC	1 cut(s)	845
FaqI	GGGAC	3 cut(s)	395, 401, 2204
FauI	CCCGC	7 cut(s)	18, 22, 161, 528, 928, 2158, 3161
FauNDI	CATATG	1 cut(s)	3085
FblI	GTMKAC	1 cut(s)	1740
FokI	GGATG	7 cut(s)	1637, 1859, 2060, 2243, 2553, 2648, 2661
FriOI	GRGCYC	2 cut(s)	2088, 2793
FseI	GGCCGGCC	1 cut(s)	655
FspBI	CTAG	5 cut(s)	909, 1004, 1218, 1812, 2271
GlaI	GCGC	2 cut(s)	861, 907
GsuI	CTGGAG	3 cut(s)	323, 1185, 2568
HaeII	RGCGCY	1 cut(s)	909
HapII	CCGG	8 cut(s)	216, 450, 473, 553, 609, 640, 652, 2364
HgaI	GACGC	4 cut(s)	449, 547, 862, 2206
HhaI	GCGC	2 cut(s)	862, 908
Hin1I	GRCGYC	1 cut(s)	441
Hin6I	GCGC	2 cut(s)	860, 906
HinP1I	GCGC	2 cut(s)	860, 906
HincII	GTYRAC	2 cut(s)	562, 1342
HindII	GTYRAC	2 cut(s)	562, 1342
HindIII	AAGCTT	1 cut(s)	1539
HpaII	CCGG	8 cut(s)	216, 450, 473, 553, 609, 640, 652, 2364
HphI	GGTGA	9 cut(s)	605, 755, 763, 769, 950, 959, 1242, 1667, 2696
Hpy166II	GTNNAC	9 cut(s)	424, 562, 760, 781, 1022, 1342, 1741, 2873, 3227
Hpy8I	GTNNAC	9 cut(s)	424, 562, 760, 781, 1022, 1342, 1741, 2873, 3227
Hpy99I	CGWCG	6 cut(s)	23, 167, 401, 404, 443, 491
HpyAV	CCTTC	8 cut(s)	16, 713, 724, 825, 1971, 2565, 2820, 2996
HpyCH4III	ACNGT	8 cut(s)	194, 712, 809, 1977, 2023, 2492, 2579, 3043
HpyCH4IV	ACGT	3 cut(s)	171, 402, 951
HpyF10VI	GCNNNNNNNGC	6 cut(s)	633, 652, 2214, 2391, 2957, 3151
HpyF3I	CTNAG	9 cut(s)	373, 690, 1332, 1581, 1646, 1785, 2626, 2760, 3283
HpySE526I	ACGT	3 cut(s)	171, 402, 951
Hsp92I	GRCGYC	1 cut(s)	441
HspAI	GCGC	2 cut(s)	860, 906
KroI	GCCGGC	2 cut(s)	215, 651
KroNI	GCCGGC	2 cut(s)	217, 653
KspI	CCGCGG	1 cut(s)	659
Kzo9I	GATC	8 cut(s)	177, 529, 1083, 1496, 1534, 1849, 2104, 2501
LmnI	GCTCC	5 cut(s)	259, 555, 2205, 2746, 2957
Lsp1109I	GCAGC	8 cut(s)	231, 648, 775, 1057, 1939, 2154, 2745, 2761
LweI	GCATC	4 cut(s)	573, 951, 1411, 1421
MabI	ACCWGGT	2 cut(s)	1252, 3238
MaeI	CTAG	5 cut(s)	909, 1004, 1218, 1812, 2271
MaeII	ACGT	3 cut(s)	171, 402, 951
MaeIII	GTNAC	7 cut(s)	260, 593, 706, 947, 1237, 2064, 2981
MalI	GATC	8 cut(s)	179, 531, 1085, 1498, 1536, 1851, 2106, 2503
MbiI	CCGCTC	1 cut(s)	1224
MboI	GATC	8 cut(s)	177, 529, 1083, 1496, 1534, 1849, 2104, 2501
MhlI	GDGCHC	2 cut(s)	2088, 2793
MlsI	TGGCCA	1 cut(s)	2913
MluNI	TGGCCA	1 cut(s)	2913
MlyI	GAGTC	7 cut(s)	237, 320, 474, 1005, 1397, 2072, 2521
MmeI	TCCRAC	2 cut(s)	867, 1081
Mox20I	TGGCCA	1 cut(s)	2913
Mph1103I	ATGCAT	2 cut(s)	966, 2392
MroNI	GCCGGC	2 cut(s)	215, 651
MscI	TGGCCA	1 cut(s)	2913
MslI	CAYNNNNRTG	2 cut(s)	1128, 2679
Msp20I	TGGCCA	1 cut(s)	2913
MspA1I	CMGCKG	1 cut(s)	658
MspI	CCGG	8 cut(s)	216, 450, 473, 553, 609, 640, 652, 2364
Mva1269I	GAATGC	2 cut(s)	805, 2167
MvaI	CCWGG	9 cut(s)	1119, 1254, 1641, 2223, 2571, 2700, 2779, 3240, 3272
MvnI	CGCG	3 cut(s)	523, 658, 1768
MwoI	GCNNNNNNNGC	6 cut(s)	633, 652, 2214, 2391, 2957, 3151
NaeI	GCCGGC	2 cut(s)	217, 653
NciI	CCSGG	4 cut(s)	450, 473, 609, 610
NdeI	CATATG	1 cut(s)	3085
NdeII	GATC	8 cut(s)	177, 529, 1083, 1496, 1534, 1849, 2104, 2501
NgoMIV	GCCGGC	2 cut(s)	215, 651
NheI	GCTAGC	1 cut(s)	1217
NlaIV	GGNNCC	3 cut(s)	1900, 2086, 2362
NmeAIII	GCCGAG	1 cut(s)	60
NmuCI	GTSAC	5 cut(s)	593, 706, 947, 2064, 2981
NsiI	ATGCAT	2 cut(s)	966, 2392
NspI	RCATGY	5 cut(s)	1330, 2037, 2192, 2394, 2992
PaeI	GCATGC	2 cut(s)	1330, 2394
PagI	TCATGA	3 cut(s)	1258, 1384, 3214
PasI	CCCWGGG	1 cut(s)	2699
PceI	AGGCCT	1 cut(s)	1002
PciI	ACATGT	2 cut(s)	2033, 2988
PcsI	WCGNNNNNNNCGW	6 cut(s)	168, 195, 299, 396, 405, 435
PctI	GAATGC	2 cut(s)	805, 2167
PdiI	GCCGGC	2 cut(s)	217, 653
PfoI	TCCNGGA	1 cut(s)	2777
PinAI	ACCGGT	1 cut(s)	2363
PleI	GAGTC	7 cut(s)	237, 320, 474, 1005, 1396, 2071, 2521
PpsI	GAGTC	7 cut(s)	237, 320, 474, 1005, 1396, 2071, 2521
PpuMI	RGGWCCY	1 cut(s)	2552
PscI	ACATGT	2 cut(s)	2033, 2988
PsiI	TTATAA	2 cut(s)	2195, 3030
Psp5II	RGGWCCY	1 cut(s)	2552
Psp6I	CCWGG	9 cut(s)	1117, 1252, 1639, 2221, 2569, 2698, 2777, 3238, 3270
PspGI	CCWGG	9 cut(s)	1117, 1252, 1639, 2221, 2569, 2698, 2777, 3238, 3270
PspN4I	GGNNCC	3 cut(s)	1900, 2086, 2362
PspOMI	GGGCCC	1 cut(s)	2084
PspPI	GGNCC	6 cut(s)	452, 1637, 1898, 2084, 2085, 2552
PspPPI	RGGWCCY	1 cut(s)	2552
PstI	CTGCAG	2 cut(s)	1368, 1986
PstNI	CAGNNNCTG	1 cut(s)	1981
RigI	GGCCGGCC	1 cut(s)	655
RsaI	GTAC	8 cut(s)	405, 1079, 1765, 1828, 1915, 1936, 2017, 2200
RsaNI	GTAC	8 cut(s)	404, 1078, 1764, 1827, 1914, 1935, 2016, 2199
RseI	CAYNNNNRTG	2 cut(s)	1128, 2679
SacII	CCGCGG	1 cut(s)	659
Sau3AI	GATC	8 cut(s)	177, 529, 1083, 1496, 1534, 1849, 2104, 2501
Sau96I	GGNCC	6 cut(s)	452, 1637, 1898, 2084, 2085, 2552
ScaI	AGTACT	3 cut(s)	1936, 2017, 2200
SchI	GAGTC	7 cut(s)	237, 320, 474, 1005, 1397, 2072, 2521
SduI	GDGCHC	2 cut(s)	2088, 2793
SexAI	ACCWGGT	2 cut(s)	1252, 3238
SfaNI	GCATC	4 cut(s)	573, 951, 1411, 1421
SfcI	CTRYAG	2 cut(s)	1364, 1982
Sfr303I	CCGCGG	1 cut(s)	659
SgrBI	CCGCGG	1 cut(s)	659
SinI	GGWCC	3 cut(s)	1637, 1898, 2552
SmaI	CCCGGG	1 cut(s)	610
SmiMI	CAYNNNNRTG	2 cut(s)	1128, 2679
SmlI	CTYRAG	2 cut(s)	986, 2413
SmoI	CTYRAG	2 cut(s)	986, 2413
SphI	GCATGC	2 cut(s)	1330, 2394
SseBI	AGGCCT	1 cut(s)	1002
SspI	AATATT	3 cut(s)	1666, 1792, 2101
SspMI	CTAG	5 cut(s)	909, 1004, 1218, 1812, 2271
StuI	AGGCCT	1 cut(s)	1002
TaaI	ACNGT	8 cut(s)	194, 712, 809, 1977, 2023, 2492, 2579, 3043
TaiI	ACGT	3 cut(s)	174, 405, 954
TaqI	TCGA	8 cut(s)	162, 189, 489, 575, 902, 1009, 1442, 1499
TatI	WGTACW	5 cut(s)	1826, 1913, 1934, 2015, 2198
TauI	GCSGC	6 cut(s)	159, 225, 519, 607, 658, 1224
TscAI	CASTG	6 cut(s)	634, 902, 1750, 2599, 2764, 3048
TseFI	GTSAC	5 cut(s)	593, 706, 947, 2064, 2981
TseI	GCWGC	8 cut(s)	219, 636, 788, 1045, 1927, 2142, 2733, 2749
Tsp45I	GTSAC	5 cut(s)	593, 706, 947, 2064, 2981
TspGWI	ACGGA	8 cut(s)	187, 475, 777, 854, 1792, 2189, 2970, 3244
TspMI	CCCGGG	1 cut(s)	608
TspRI	CASTG	6 cut(s)	634, 902, 1750, 2599, 2764, 3048
VpaK11BI	GGWCC	3 cut(s)	1637, 1898, 2552
XapI	RAATTY	3 cut(s)	1291, 1301, 2831
XceI	RCATGY	5 cut(s)	1330, 2037, 2192, 2394, 2992
XmaI	CCCGGG	1 cut(s)	608
XmiI	GTMKAC	1 cut(s)	1740
XspI	CTAG	5 cut(s)	909, 1004, 1218, 1812, 2271
ZrmI	AGTACT	3 cut(s)	1936, 2017, 2200
Zsp2I	ATGCAT	2 cut(s)	966, 2392

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.