Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0003067

Physical Location & Seq

Genomic Coordinates Reverse (-)

4384152 .. 4391409

Gene Structure 7258 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00221630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3549 bp

ATGGCCACCGTCGACGACCACCACCTCCTCCACCTGGACTCCGTCCCCGTCGTCGACCTCCGCCTCCTCACCCAATCCGAGCTCTACTCCCTCTCCCTCTCCTCCTCCTCCTCCTCCGCATCTCACCGCTTCGACGACGACGTTTTGATCCCCAAAATCGACCGCTCCGTCTTCAACGAATCCGCCGGCAGCCGCAAACAGACCTACTCCCGCCTCCGCCTCGCCCCTCGCAACTCCCAATTCGCCTCCTCCAATTCCAAATCTGCCCTCCCTCCCTCCCACCTCCTCCTCTCCCAGCCGCTCGATCACGAGAGCCGCCAGATTATCTCGCTCCTCAAGCAGCTCTTCCCCTCCGACGATCAAAACGACGACGTCCTCCTCCCCCAAACCACCACCACCACCACCCCCGTGCAGTACAAAATCCCCGCCGCGCCGGCGCCTTCCCTCCCTGCCTCGGCCGGTGCGGTTTCCGGCGGCGTCGATTCCTCGCTCGGGAAACGGAAGCGCGGCCGGCCGCGGAAGGATTCCAACGCCGTGATTGCCAAATCGGTGGCGGCTGTGCTGGTCGGCTCTGCTGCGTTGGCCGGCGGTGCCCGCGAGGCTGAGGATGGCAATGCCGGTGTTTTGATTGAGAAGCGTGACGGTGGAGCTACGGCGGTTGGGACTCTTGTTTTGAATAGCGAAGGACAGCTTGTGCCGAGTAGCGAAGGGAAGAGGAAGCGAGGGCGGCCGCGGAAGGATGAGATTAGGGTTAGGGATGAGAGTACAGTGGCGGCTTCGGTGAGGAAACCGCCGAGAGTGAAGAAGGAGGTGAAGGAGGAGGATGGAGAGATGGCGATGGTGAACGTGAACGACGTCGTTGTGGATTTGGAAGCATTGGCCAATGCGGACAGTGTGTTTGGGGAGGAGTTGAGGAAGAGGACAGAGGGGTTGAAGACTGAGGCGCAGTTGTTAGGGTTTCTGGAAGGGTTGGAGGGGGACTGGTCAAGTGCTAGGAAGAAGAGGAAGATTGTGCAGGCGAGTGAGCTCGGCGACTTGTTGCCAAAGTGGTGGAAGATCATGCTGTCTCTCAAGAGGAATGGAGGTCATAAATGGCTTGTGTGTAGGCGGTTCATAAGCCCTAATGGGTTGGAGTTTGTGTCGTGCAAGGAAGTTTCTTCATACTTGCACTCTTATTGTGGACTTCGAAGCCAGTCGAATTCTGTTCATACCGATGGGAATATTCAGGTTTCTAATAAAATGGTTTTGGGAGATGGAATGCAACATGATACTAATTTTACCTATAAAGATAACAACAATGGTCATGAGCTTCTTTGCTGCTCCCCAATGCCCATTACCTCTATGAACAGCTATGTCAAGAGCATTGAAGCATCAGGAGAAGTAATGGGTATGCGAAAAAAATTGGATCCTGTAAAGAATGGTCGTTTGGGGACATCCTTGAAAATTCAAGAACCAGTTGAGATAGATGGGGAACAAACTTCTGATATGATAGCCAACTATGATTTGAGTTCTGATTTTTCTCTGGGTAAGGTAAATGATATCATGGCAAAATATTTTGGCATATCCAATGATGAATCGAATTCAATGTCCTGTGAGGAGCAAGTGATGATGGCTGACAAAACAATTTGCTTTTCTGATAAATTAAATGATGGTGTCAGGGTTGCCATCAGCTCAGTTGATGTGCCCAAAGTGGAGATAGACAGTGCTTCAAATGACATGGGAAAAGAACATGGAGCTGACAATATTGATAATCACCAATCAACTAGCACTGTAGATGATATGAAAATTGATGATGTGGATAATTGTAGGAATGGCAGGTCAATATCTGGTATTAGTGAGAGTCACATTGGACCTGAGACTGCATATAATGTTGACCAACAAAGTACTTCAGAAGCCTGCTCGCTTGTTCCTTCAATTGTGATGGAGTCTCTTCATGAAAGAGGTTCCGACAGTGGTTTATATAGTACAGCCACTGATGAGAAAACATGTGTTGTCAGTAATAACTTCAATAATGATTCATTCCCCACATTTTATGAGCCCAGATTTGATGATATTGGTCTATCCGGGAGTAATGAGAAGACATTTTGTATTGGCAGTGATCATGCTATGCCAGATGTAGATTCCATGGAGATCAGTGAGCGAATTCGAAGTACTGGAAGCTATTCAAATGTTCCACCTCTGGATGGACATAGTTGTATTATTAATGCAACGAATCCCAGTTGCACAGTGGAAGAACTCTTGCAGGACAAGAGTTTAGCAAGCAACGCATTTAATTCATTTGTTAATAAGCAGACCCCTGGTAATCTAAATAAGGGTGCTATTCATACAGCAGAAGGGCATAAAGTTGAGCATGCCAAAAATTATGACAATAGTGAGTTAAGTGTTAATTTTGGTAGCAGGGCCACTGGAATTAATGCAGATACTACCTCTTGCATTGACCAGGAAAGAAGCTCTAAAGGCTTTTCACTTGCTTCATATGCAAACGAGCATGCATCTGTTTCGGAAAATAGTTTTGTCTGCAATAGCATTTTGGAAGAACTTAAGCCCGATAGAGGTACTTCTGAAACTGATTTGGTTTGTCCGTCGGAATATCAACGAGGATATAATCTGGTAAATCATGCAAACAACACATCCTCAAGGCCAATTGAAGTATTAAATGAGGAGGTTAAAAGATCTTGGAATGATGAGGTGCTGCATACTTTTGGCAGCAGTCGCAGTGGACAAGAAGTGAATGCTATGAGTAGCACTGTGACCTCAATAAATTCTCAAGGACCTTCCCTTGTTATTTCGGAGGATAACCAATCATTTGCTACTGGCTCTGGCATATTTAGTAGCTCAGTATTGGACGATAAGCTGATAAGAGTCCCTGTTAATGGCTTAGCACGCCCAAGTGGTAGTGAGCAAACCCCTGATTTTGTAAATAATCTGAACAGGATTTATAGCACCACATCGTGGGAGCAGCCTAAATCCGAGGTTGCAGAAAATTCAAGGAACAATCAGGCCATCAATGGTTTTTTAAATGATTCCCAACCTACTGCTGATTCCATCTCCGAGCTTAGGTGGAGAAATGTTGGGCAAAATGTCCAGCAGAGTGGATTGGCTGACAATTCTTCTGCACTTATGCCATCATCTGTCAGTTATCCGAACTTTGATCTGCTGTCAGGCAAGGGTGCAAATGGAGCTTTGAGCATAAGTGAAAAGTTTGACAACACTTCATGTTTGGAAGGATTAAAGTCAAGTAGATTAGAGCAGTTTGAGCATGACTTCCTGACTGCTCAAGCAACCTCTCAGTCGAAGGAATCAAATGTGTTGTCATATGATGAGACTCATGAGAGTATAGAGCAAGGTTTCAATTCATCTGGTTGGCCAGAGAAGGAAGCACTGCCTTCGGTGCCAAATATAGAAAGCAGGCGTGAGATTAACAGCTGTGTCTGGTGCAGAAATGAGTTTTATCATGATGTTTATGGAGCCGGAATGCAAACTGGTTCAGTGGGTTTTATGTGTGCAAACTGCCAGGGCAAGTTCTCAGGTCATGTCGGTTTTCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0008327 GO:0036094 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AT_hook MBD

Protein Analysis

1182

Amino Acids

128.53

Weight (kDa)

5.12

Isoelectric Point (pI)

48.96

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003067)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120 FvH4_5g37120
malus_domestica	MD00G1009700.v1.1 MD08G1184300.v1.1 MD15G1370800.v1.1
prunus_persica	Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1 Prupe.1G518500_v2.0.a1
pyrus_communis	pycom08g15800
rosa_chinensis	RchiOBHm_Chr7g0238671 RchiOBHm_Chr7g0243951 RchiOBHm_Chr7g0243961
rosa_laevigata	RLG00000000820
rosa_multiflora	Rmu_sc0006110.1_g000008
rosa_roxburghii	Rroxscaffold_3G00221630
rosa_rugosa	Rorug07G0322800
rosa_samantha	Rh7AG477200 Rh7BG448500 Rh7CG495300 Rh7DG462200
rosa_wichuraiana	Rw7G039830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	167
AatII	GACGTC	2 cut(s)	375, 858
Acc36I	ACCTGC	1 cut(s)	1806
AccB1I	GGYRCC	3 cut(s)	436, 590, 3391
AccB7I	CCANNNNNTGG	1 cut(s)	2952
AccBSI	CCGCTC	2 cut(s)	165, 301
AccI	GTMKAC	2 cut(s)	12, 54
AccII	CGCG	5 cut(s)	431, 507, 517, 597, 733
AclWI	GGATC	3 cut(s)	142, 1400, 1413
AcoI	YGGCCR	8 cut(s)	3, 456, 508, 512, 582, 728, 879, 3365
AcsI	RAATTY	6 cut(s)	1198, 1443, 1579, 2142, 2761, 2983
AcuI	CTGAAG	1 cut(s)	1872
AcyI	GRCGYC	4 cut(s)	372, 437, 477, 855
AdeI	CACNNNGTG	1 cut(s)	2952
AfaI	GTAC	6 cut(s)	416, 766, 1885, 1966, 2152, 2557
AfiI	CCNNNNNNNGG	8 cut(s)	34, 454, 492, 2183, 2552, 2873, 2952, 3057
AflII	CTTAAG	1 cut(s)	2540
AflIII	ACRYGT	1 cut(s)	1985
AjnI	CCWGG	4 cut(s)	33, 2296, 2439, 3513
AjuI	GAANNNNNNNTTGG	2 cut(s)	1409, 1441
AleI	CACNNNNGTG	1 cut(s)	407
Alw21I	GWGCWC	2 cut(s)	84, 1029
Alw26I	GTCTC	4 cut(s)	1071, 1850, 1932, 3317
AlwI	GGATC	3 cut(s)	142, 1400, 1413
AlwNI	CAGNNNCTG	1 cut(s)	1973
Ama87I	CYCGRG	1 cut(s)	491
ApeKI	GCWGC	7 cut(s)	189, 340, 575, 1317, 2692, 2706, 2959
ApoI	RAATTY	6 cut(s)	1198, 1443, 1579, 2142, 2761, 2983
ArsI	GACNNNNNNTTYG	8 cut(s)	655, 687, 881, 913, 1571, 1603, 3296, 3328
AseI	ATTAAT	2 cut(s)	2202, 2412
Asp700I	GAANNNNTTC	1 cut(s)	2161
AspLEI	GCGC	4 cut(s)	433, 439, 507, 946
AspS9I	GGNCC	3 cut(s)	1850, 2400, 2771
AsuC2I	CCSGG	1 cut(s)	2065
AsuHPI	GGTGA	6 cut(s)	61, 116, 793, 823, 853, 1745
AsuII	TTCGAA	2 cut(s)	1186, 2146
AvaI	CYCGRG	1 cut(s)	491
AvaII	GGWCC	2 cut(s)	1850, 2771
BaeGI	GKGCMC	2 cut(s)	595, 1686
BalI	TGGCCA	3 cut(s)	5, 881, 3367
BamHI	GGATCC	1 cut(s)	1405
BanI	GGYRCC	3 cut(s)	436, 590, 3391
BanII	GRGCYC	3 cut(s)	84, 1029, 2040
BauI	CACGAG	1 cut(s)	308
BbsI	GAAGAC	3 cut(s)	163, 941, 2084
Bbv12I	GWGCWC	2 cut(s)	84, 1029
BbvCI	CCTCAGC	1 cut(s)	603
BbvI	GCAGC	7 cut(s)	201, 352, 562, 1304, 2679, 2718, 2971
BceAI	ACGGC	2 cut(s)	518, 669
BcgI	CGANNNNNNTGC	4 cut(s)	2481, 2515, 3369, 3403
BciT130I	CCWGG	4 cut(s)	35, 2298, 2441, 3515
BclI	TGATCA	1 cut(s)	2098
BcnI	CCSGG	1 cut(s)	2065
BcoDI	GTCTC	4 cut(s)	1071, 1850, 1932, 3317
BfaI	CTAG	2 cut(s)	993, 1764
BfmI	CTRYAG	2 cut(s)	1770, 3545
BfoI	RGCGCY	1 cut(s)	440
BfrI	CTTAAG	1 cut(s)	2540
BfuAI	ACCTGC	1 cut(s)	1806
BglI	GCCNNNNNGGC	2 cut(s)	434, 599
BglII	AGATCT	1 cut(s)	2672
BlpI	GCTNAGC	1 cut(s)	2878
BmcAI	AGTACT	2 cut(s)	1885, 2152
Bme1390I	CCNGG	5 cut(s)	35, 2065, 2298, 2441, 3515
Bme18I	GGWCC	2 cut(s)	1850, 2771
BmeT110I	CYCGRG	1 cut(s)	491
BmgT120I	GGNCC	3 cut(s)	1850, 2400, 2771
BmiI	GGNNCC	6 cut(s)	438, 592, 1407, 1945, 3393, 3469
BmrFI	CCNGG	5 cut(s)	35, 2065, 2298, 2441, 3515
BmrI	ACTGGG	1 cut(s)	2211
BmsI	GCATC	3 cut(s)	128, 1379, 2501
BmuI	ACTGGG	1 cut(s)	2211
BpiI	GAAGAC	3 cut(s)	163, 941, 2084
BplI	GAGNNNNNCTC	2 cut(s)	71, 103
Bpu10I	CCTNAGC	2 cut(s)	603, 3056
Bpu1102I	GCTNAGC	1 cut(s)	2878
Bpu14I	TTCGAA	2 cut(s)	1186, 2146
BpuEI	CTTGAG	5 cut(s)	320, 1055, 2620, 2751, 3261
BpuMI	CCSGG	1 cut(s)	2065
BsaHI	GRCGYC	4 cut(s)	372, 437, 477, 855
BsaJI	CCNNGG	7 cut(s)	453, 515, 731, 2124, 2296, 2970, 3514
BsaXI	ACNNNNNCTCC	6 cut(s)	23, 53, 77, 107, 1124, 1154
Bsc4I	CCNNNNNNNGG	8 cut(s)	34, 454, 492, 2183, 2552, 2873, 2952, 3057
Bse118I	RCCGGY	6 cut(s)	185, 433, 458, 510, 584, 617
Bse1I	ACTGG	8 cut(s)	986, 1192, 1454, 2158, 2217, 2410, 2818, 3487
Bse3DI	GCAATG	1 cut(s)	619
BseBI	CCWGG	4 cut(s)	35, 2298, 2441, 3515
BseDI	CCNNGG	7 cut(s)	453, 515, 731, 2124, 2296, 2970, 3514
BseGI	GGATG	7 cut(s)	613, 745, 763, 829, 1433, 2188, 2630
BseLI	CCNNNNNNNGG	8 cut(s)	34, 454, 492, 2183, 2552, 2873, 2952, 3057
BseMI	GCAATG	1 cut(s)	619
BseMII	CTCAG	7 cut(s)	594, 930, 1686, 1845, 2850, 3302, 3540
BseNI	ACTGG	8 cut(s)	986, 1192, 1454, 2158, 2217, 2410, 2818, 3487
BseSI	GKGCMC	2 cut(s)	595, 1686
BseX3I	CGGCCG	4 cut(s)	456, 508, 512, 728
BseXI	GCAGC	7 cut(s)	201, 352, 562, 1304, 2679, 2718, 2971
BseYI	CCCAGC	1 cut(s)	294
BsgI	GTGCAG	4 cut(s)	431, 1034, 3099, 3457
Bsh1236I	CGCG	5 cut(s)	431, 507, 517, 597, 733
Bsh1285I	CGRYCG	5 cut(s)	163, 459, 511, 515, 731
BshNI	GGYRCC	3 cut(s)	436, 590, 3391
BsiEI	CGRYCG	5 cut(s)	163, 459, 511, 515, 731
BsiHKAI	GWGCWC	2 cut(s)	84, 1029
BsiHKCI	CYCGRG	1 cut(s)	491
BsiSI	CCGG	9 cut(s)	186, 434, 459, 471, 511, 585, 618, 2064, 3471
BslFI	GGGAC	5 cut(s)	29, 676, 992, 1444, 2849
BslI	CCNNNNNNNGG	8 cut(s)	34, 454, 492, 2183, 2552, 2873, 2952, 3057
BsmAI	GTCTC	4 cut(s)	1071, 1850, 1932, 3317
BsmFI	GGGAC	5 cut(s)	29, 676, 992, 1444, 2849
BsmI	GAATGC	3 cut(s)	1263, 2737, 3480
BsoBI	CYCGRG	1 cut(s)	491
Bsp119I	TTCGAA	2 cut(s)	1186, 2146
Bsp1286I	GDGCHC	5 cut(s)	84, 595, 1029, 1686, 2040
Bsp143I	GATC	9 cut(s)	147, 304, 358, 1056, 1405, 2098, 2130, 2672, 3151
Bsp1720I	GCTNAGC	1 cut(s)	2878
Bsp19I	CCATGG	1 cut(s)	2124
BspCNI	CTCAG	7 cut(s)	595, 931, 1685, 1846, 2849, 3301, 3539
BspFNI	CGCG	5 cut(s)	431, 507, 517, 597, 733
BspHI	TCATGA	4 cut(s)	1303, 1933, 3328, 3454
BspLI	GGNNCC	6 cut(s)	438, 592, 1407, 1945, 3393, 3469
BspMI	ACCTGC	1 cut(s)	1806
BspPI	GGATC	3 cut(s)	142, 1400, 1413
BspQI	GCTCTTC	1 cut(s)	350
BspT104I	TTCGAA	2 cut(s)	1186, 2146
BspT107I	GGYRCC	3 cut(s)	436, 590, 3391
BspTI	CTTAAG	1 cut(s)	2540
BsrBI	CCGCTC	2 cut(s)	165, 301
BsrDI	GCAATG	1 cut(s)	619
BsrFI	RCCGGY	6 cut(s)	185, 433, 458, 510, 584, 617
BsrI	ACTGG	8 cut(s)	986, 1192, 1454, 2158, 2217, 2410, 2818, 3487
BssAI	RCCGGY	6 cut(s)	185, 433, 458, 510, 584, 617
BssECI	CCNNGG	7 cut(s)	453, 515, 731, 2124, 2296, 2970, 3514
BssMI	GATC	9 cut(s)	147, 304, 358, 1056, 1405, 2098, 2130, 2672, 3151
BssNI	GRCGYC	4 cut(s)	372, 437, 477, 855
BssSI	CACGAG	1 cut(s)	308
BssT1I	CCWWGG	1 cut(s)	2124
Bst2BI	CACGAG	1 cut(s)	308
Bst2UI	CCWGG	4 cut(s)	35, 2298, 2441, 3515
Bst4CI	ACNGT	9 cut(s)	10, 644, 769, 893, 1703, 1771, 1952, 2227, 2749
Bst6I	CTCTTC	5 cut(s)	350, 707, 911, 995, 1935
BstACI	GRCGYC	4 cut(s)	372, 437, 477, 855
BstAFI	CTTAAG	1 cut(s)	2540
BstBI	TTCGAA	2 cut(s)	1186, 2146
BstDEI	CTNAG	9 cut(s)	603, 939, 1672, 1854, 2836, 2878, 3056, 3288, 3526
BstDSI	CCRYGG	3 cut(s)	515, 731, 2124
BstF5I	GGATG	7 cut(s)	613, 745, 763, 829, 1433, 2188, 2630
BstFNI	CGCG	5 cut(s)	431, 507, 517, 597, 733
BstH2I	RGCGCY	1 cut(s)	440
BstHHI	GCGC	4 cut(s)	433, 439, 507, 946
BstKTI	GATC	9 cut(s)	150, 307, 361, 1059, 1408, 2101, 2133, 2675, 3154
BstMAI	GTCTC	4 cut(s)	1071, 1850, 1932, 3317
BstMBI	GATC	9 cut(s)	147, 304, 358, 1056, 1405, 2098, 2130, 2672, 3151
BstMCI	CGRYCG	5 cut(s)	163, 459, 511, 515, 731
BstNI	CCWGG	4 cut(s)	35, 2298, 2441, 3515
BstNSI	RCATGY	3 cut(s)	1989, 2354, 2492
BstSCI	CCNGG	5 cut(s)	33, 2063, 2296, 2439, 3513
BstSFI	CTRYAG	2 cut(s)	1770, 3545
BstSLI	GKGCMC	2 cut(s)	595, 1686
BstUI	CGCG	5 cut(s)	431, 507, 517, 597, 733
BstV1I	GCAGC	7 cut(s)	201, 352, 562, 1304, 2679, 2718, 2971
BstV2I	GAAGAC	3 cut(s)	163, 941, 2084
BstX2I	RGATCY	2 cut(s)	1405, 2672
BstXI	CCANNNNNNTGG	3 cut(s)	550, 1050, 3092
BstYI	RGATCY	2 cut(s)	1405, 2672
BstZI	CGGCCG	4 cut(s)	456, 508, 512, 728
BtgI	CCRYGG	3 cut(s)	515, 731, 2124
BtgZI	GCGATG	1 cut(s)	851
BtsCI	GGATG	7 cut(s)	613, 745, 763, 829, 1433, 2188, 2630
BtsI	GCAGTG	3 cut(s)	2101, 2722, 3380
BveI	ACCTGC	1 cut(s)	1806
CaiI	CAGNNNCTG	1 cut(s)	1973
CciI	TCATGA	4 cut(s)	1303, 1933, 3328, 3454
CciNI	GCGGCCGC	1 cut(s)	728
CfoI	GCGC	4 cut(s)	433, 439, 507, 946
Cfr10I	RCCGGY	6 cut(s)	185, 433, 458, 510, 584, 617
Cfr13I	GGNCC	3 cut(s)	1850, 2400, 2771
Cfr42I	CCGCGG	2 cut(s)	518, 734
CseI	GACGC	1 cut(s)	466
Csp6I	GTAC	6 cut(s)	415, 765, 1884, 1965, 2151, 2556
CviQI	GTAC	6 cut(s)	415, 765, 1884, 1965, 2151, 2556
DdeI	CTNAG	9 cut(s)	603, 939, 1672, 1854, 2836, 2878, 3056, 3288, 3526
DinI	GGCGCC	1 cut(s)	438
DpnI	GATC	9 cut(s)	149, 306, 360, 1058, 1407, 2100, 2132, 2674, 3153
DpnII	GATC	9 cut(s)	147, 304, 358, 1056, 1405, 2098, 2130, 2672, 3151
DraI	TTTAAA	1 cut(s)	3018
DraIII	CACNNNGTG	1 cut(s)	2952
DrdI	GACNNNNNNGTC	1 cut(s)	167
DseDI	GACNNNNNNGTC	1 cut(s)	167
EaeI	YGGCCR	8 cut(s)	3, 456, 508, 512, 582, 728, 879, 3365
EagI	CGGCCG	4 cut(s)	456, 508, 512, 728
Eam1104I	CTCTTC	5 cut(s)	350, 707, 911, 995, 1935
EarI	CTCTTC	5 cut(s)	350, 707, 911, 995, 1935
EciI	GGCGGA	3 cut(s)	50, 172, 206
Ecl136II	GAGCTC	2 cut(s)	82, 1027
EclXI	CGGCCG	4 cut(s)	456, 508, 512, 728
Eco130I	CCWWGG	1 cut(s)	2124
Eco24I	GRGCYC	3 cut(s)	84, 1029, 2040
Eco32I	GATATC	1 cut(s)	1540
Eco47I	GGWCC	2 cut(s)	1850, 2771
Eco52I	CGGCCG	4 cut(s)	456, 508, 512, 728
Eco53kI	GAGCTC	2 cut(s)	82, 1027
Eco57I	CTGAAG	1 cut(s)	1872
Eco88I	CYCGRG	1 cut(s)	491
EcoICRI	GAGCTC	2 cut(s)	82, 1027
EcoO109I	RGGNCCY	1 cut(s)	2771
EcoRI	GAATTC	3 cut(s)	1198, 1579, 2142
EcoRII	CCWGG	4 cut(s)	33, 2296, 2439, 3513
EcoRV	GATATC	1 cut(s)	1540
EcoT14I	CCWWGG	1 cut(s)	2124
EcoT22I	ATGCAT	1 cut(s)	2494
EcoT38I	GRGCYC	3 cut(s)	84, 1029, 2040
EgeI	GGCGCC	1 cut(s)	438
EheI	GGCGCC	1 cut(s)	438
ErhI	CCWWGG	1 cut(s)	2124
FalI	AAGNNNNNCTT	4 cut(s)	329, 361, 675, 707
FaqI	GGGAC	5 cut(s)	29, 676, 992, 1444, 2849
FauI	CCCGC	3 cut(s)	218, 433, 602
FauNDI	CATATG	2 cut(s)	2476, 3316
FbaI	TGATCA	1 cut(s)	2098
FblI	GTMKAC	2 cut(s)	12, 54
FokI	GGATG	7 cut(s)	620, 752, 770, 836, 1420, 2195, 2617
FriOI	GRGCYC	3 cut(s)	84, 1029, 2040
FseI	GGCCGGCC	1 cut(s)	514
FspBI	CTAG	2 cut(s)	993, 1764
GlaI	GCGC	4 cut(s)	432, 438, 506, 945
GsaI	CCCAGC	1 cut(s)	298
HaeII	RGCGCY	1 cut(s)	440
HapII	CCGG	9 cut(s)	186, 434, 459, 471, 511, 585, 618, 2064, 3471
HgaI	GACGC	1 cut(s)	466
HhaI	GCGC	4 cut(s)	433, 439, 507, 946
Hin1I	GRCGYC	4 cut(s)	372, 437, 477, 855
Hin6I	GCGC	4 cut(s)	431, 437, 505, 944
HinP1I	GCGC	4 cut(s)	431, 437, 505, 944
HincII	GTYRAC	3 cut(s)	13, 55, 1873
HindII	GTYRAC	3 cut(s)	13, 55, 1873
HpaII	CCGG	9 cut(s)	186, 434, 459, 471, 511, 585, 618, 2064, 3471
HphI	GGTGA	6 cut(s)	61, 116, 793, 823, 853, 1745
Hpy166II	GTNNAC	7 cut(s)	13, 55, 844, 850, 1181, 1873, 2720
Hpy8I	GTNNAC	7 cut(s)	13, 55, 844, 850, 1181, 1873, 2720
HpyCH4III	ACNGT	9 cut(s)	10, 644, 769, 893, 1703, 1771, 1952, 2227, 2749
HpyCH4IV	ACGT	4 cut(s)	141, 372, 846, 855
HpyF3I	CTNAG	9 cut(s)	603, 939, 1672, 1854, 2836, 2878, 3056, 3288, 3526
HpySE526I	ACGT	4 cut(s)	141, 372, 846, 855
Hsp92I	GRCGYC	4 cut(s)	372, 437, 477, 855
HspAI	GCGC	4 cut(s)	431, 437, 505, 944
KasI	GGCGCC	1 cut(s)	436
KroI	GCCGGC	4 cut(s)	185, 433, 510, 584
KroNI	GCCGGC	4 cut(s)	187, 435, 512, 586
Ksp22I	TGATCA	1 cut(s)	2098
KspI	CCGCGG	2 cut(s)	518, 734
Kzo9I	GATC	9 cut(s)	147, 304, 358, 1056, 1405, 2098, 2130, 2672, 3151
LguI	GCTCTTC	1 cut(s)	350
LmnI	GCTCC	9 cut(s)	170, 336, 647, 1325, 1597, 1733, 2956, 3179, 3467
Lsp1109I	GCAGC	7 cut(s)	201, 352, 562, 1304, 2679, 2718, 2971
LweI	GCATC	3 cut(s)	128, 1379, 2501
MaeI	CTAG	2 cut(s)	993, 1764
MaeII	ACGT	4 cut(s)	141, 372, 846, 855
MaeIII	GTNAC	3 cut(s)	638, 1841, 2749
MalI	GATC	9 cut(s)	149, 306, 360, 1058, 1407, 2100, 2132, 2674, 3153
MbiI	CCGCTC	2 cut(s)	165, 301
MboI	GATC	9 cut(s)	147, 304, 358, 1056, 1405, 2098, 2130, 2672, 3151
MfeI	CAATTG	2 cut(s)	1914, 2643
MflI	RGATCY	2 cut(s)	1405, 2672
MhlI	GDGCHC	5 cut(s)	84, 595, 1029, 1686, 2040
MlsI	TGGCCA	3 cut(s)	5, 881, 3367
MluNI	TGGCCA	3 cut(s)	5, 881, 3367
Mly113I	GGCGCC	1 cut(s)	437
MlyI	GAGTC	6 cut(s)	32, 658, 1849, 1934, 2871, 3319
MmeI	TCCRAC	6 cut(s)	378, 552, 951, 1110, 1971, 2565
Mox20I	TGGCCA	3 cut(s)	5, 881, 3367
Mph1103I	ATGCAT	1 cut(s)	2494
MreI	CGCCGGCG	1 cut(s)	433
MroNI	GCCGGC	4 cut(s)	185, 433, 510, 584
MroXI	GAANNNNTTC	1 cut(s)	2161
MscI	TGGCCA	3 cut(s)	5, 881, 3367
MslI	CAYNNNNRTG	2 cut(s)	407, 1212
Msp20I	TGGCCA	3 cut(s)	5, 881, 3367
MspA1I	CMGCKG	3 cut(s)	517, 733, 3426
MspCI	CTTAAG	1 cut(s)	2540
MspI	CCGG	9 cut(s)	186, 434, 459, 471, 511, 585, 618, 2064, 3471
MspR9I	CCNGG	5 cut(s)	35, 2065, 2298, 2441, 3515
MunI	CAATTG	2 cut(s)	1914, 2643
Mva1269I	GAATGC	3 cut(s)	1263, 2737, 3480
MvaI	CCWGG	4 cut(s)	35, 2298, 2441, 3515
MvnI	CGCG	5 cut(s)	431, 507, 517, 597, 733
NaeI	GCCGGC	4 cut(s)	187, 435, 512, 586
NarI	GGCGCC	1 cut(s)	437
NciI	CCSGG	1 cut(s)	2065
NcoI	CCATGG	1 cut(s)	2124
NdeI	CATATG	2 cut(s)	2476, 3316
NdeII	GATC	9 cut(s)	147, 304, 358, 1056, 1405, 2098, 2130, 2672, 3151
NgoMIV	GCCGGC	4 cut(s)	185, 433, 510, 584
NlaIV	GGNNCC	6 cut(s)	438, 592, 1407, 1945, 3393, 3469
NmeAIII	GCCGAG	4 cut(s)	434, 723, 819, 1008
NmuCI	GTSAC	3 cut(s)	638, 1841, 2749
NotI	GCGGCCGC	1 cut(s)	728
NsiI	ATGCAT	1 cut(s)	2494
NspI	RCATGY	3 cut(s)	1989, 2354, 2492
NspV	TTCGAA	2 cut(s)	1186, 2146
OliI	CACNNNNGTG	1 cut(s)	407
PaeI	GCATGC	2 cut(s)	2354, 2492
PagI	TCATGA	4 cut(s)	1303, 1933, 3328, 3454
PciI	ACATGT	1 cut(s)	1985
PciSI	GCTCTTC	1 cut(s)	350
PcsI	WCGNNNNNNNCGW	4 cut(s)	138, 165, 363, 852
PctI	GAATGC	3 cut(s)	1263, 2737, 3480
PdiI	GCCGGC	4 cut(s)	187, 435, 512, 586
PdmI	GAANNNNTTC	1 cut(s)	2161
PflFI	GACNNNGTC	2 cut(s)	41, 371
PflMI	CCANNNNNTGG	1 cut(s)	2952
PfoI	TCCNGGA	1 cut(s)	2063
PleI	GAGTC	6 cut(s)	32, 658, 1848, 1933, 2870, 3319
PluTI	GGCGCC	1 cut(s)	440
PpsI	GAGTC	6 cut(s)	32, 658, 1848, 1933, 2870, 3319
PpuMI	RGGWCCY	1 cut(s)	2771
PscI	ACATGT	1 cut(s)	1985
PshBI	ATTAAT	2 cut(s)	2202, 2412
Psp124BI	GAGCTC	2 cut(s)	84, 1029
Psp5II	RGGWCCY	1 cut(s)	2771
Psp6I	CCWGG	4 cut(s)	33, 2296, 2439, 3513
PspFI	CCCAGC	1 cut(s)	294
PspGI	CCWGG	4 cut(s)	33, 2296, 2439, 3513
PspN4I	GGNNCC	6 cut(s)	438, 592, 1407, 1945, 3393, 3469
PspPI	GGNCC	3 cut(s)	1850, 2400, 2771
PspPPI	RGGWCCY	1 cut(s)	2771
PstNI	CAGNNNCTG	1 cut(s)	1973
PsuI	RGATCY	2 cut(s)	1405, 2672
PsyI	GACNNNGTC	2 cut(s)	41, 371
PvuII	CAGCTG	1 cut(s)	3426
RigI	GGCCGGCC	1 cut(s)	514
RsaI	GTAC	6 cut(s)	416, 766, 1885, 1966, 2152, 2557
RsaNI	GTAC	6 cut(s)	415, 765, 1884, 1965, 2151, 2556
RseI	CAYNNNNRTG	2 cut(s)	407, 1212
SacI	GAGCTC	2 cut(s)	84, 1029
SacII	CCGCGG	2 cut(s)	518, 734
SalI	GTCGAC	2 cut(s)	11, 53
SapI	GCTCTTC	1 cut(s)	350
Sau3AI	GATC	9 cut(s)	147, 304, 358, 1056, 1405, 2098, 2130, 2672, 3151
Sau96I	GGNCC	3 cut(s)	1850, 2400, 2771
ScaI	AGTACT	2 cut(s)	1885, 2152
SchI	GAGTC	6 cut(s)	32, 658, 1849, 1934, 2871, 3319
ScrFI	CCNGG	5 cut(s)	35, 2065, 2298, 2441, 3515
SduI	GDGCHC	5 cut(s)	84, 595, 1029, 1686, 2040
SfaNI	GCATC	3 cut(s)	128, 1379, 2501
SfcI	CTRYAG	2 cut(s)	1770, 3545
SfoI	GGCGCC	1 cut(s)	438
Sfr303I	CCGCGG	2 cut(s)	518, 734
SfuI	TTCGAA	2 cut(s)	1186, 2146
SgrAI	CRCCGGYG	1 cut(s)	433
SgrBI	CCGCGG	2 cut(s)	518, 734
SgrDI	CGTCGACG	1 cut(s)	11
SinI	GGWCC	2 cut(s)	1850, 2771
SmiMI	CAYNNNNRTG	2 cut(s)	407, 1212
SmlI	CTYRAG	6 cut(s)	335, 1070, 2540, 2635, 2766, 3276
SmoI	CTYRAG	6 cut(s)	335, 1070, 2540, 2635, 2766, 3276
SphI	GCATGC	2 cut(s)	2354, 2492
SspDI	GGCGCC	1 cut(s)	436
SspI	AATATT	3 cut(s)	1222, 1553, 1744
SspMI	CTAG	2 cut(s)	993, 1764
SstI	GAGCTC	2 cut(s)	84, 1029
StyD4I	CCNGG	5 cut(s)	33, 2063, 2296, 2439, 3513
StyI	CCWWGG	1 cut(s)	2124
TaaI	ACNGT	9 cut(s)	10, 644, 769, 893, 1703, 1771, 1952, 2227, 2749
TaiI	ACGT	4 cut(s)	144, 375, 849, 858
TatI	WGTACW	5 cut(s)	414, 764, 1883, 1964, 2150
TseFI	GTSAC	3 cut(s)	638, 1841, 2749
TseI	GCWGC	7 cut(s)	189, 340, 575, 1317, 2692, 2706, 2959
Tsp45I	GTSAC	3 cut(s)	638, 1841, 2749
TspGWI	ACGGA	4 cut(s)	31, 157, 514, 2571
Tth111I	GACNNNGTC	2 cut(s)	41, 371
Van91I	CCANNNNNTGG	1 cut(s)	2952
Vha464I	CTTAAG	1 cut(s)	2540
VpaK11BI	GGWCC	2 cut(s)	1850, 2771
VspI	ATTAAT	2 cut(s)	2202, 2412
XapI	RAATTY	6 cut(s)	1198, 1443, 1579, 2142, 2761, 2983
XceI	RCATGY	3 cut(s)	1989, 2354, 2492
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2224
XmiI	GTMKAC	2 cut(s)	12, 54
XmnI	GAANNNNTTC	1 cut(s)	2161
XspI	CTAG	2 cut(s)	993, 1764
ZraI	GACGTC	2 cut(s)	373, 856
ZrmI	AGTACT	2 cut(s)	1885, 2152
Zsp2I	ATGCAT	1 cut(s)	2494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.