Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0011428

Physical Location & Seq

Genomic Coordinates Reverse (-)

25376069 .. 25378757

Gene Structure 2689 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1196700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 945 bp

ATGGATCCAAACAGGGTGGAAGCCGAGCGATTGCTCGGAATCGCCGAGAAGCTTCTACAGAGCCGGGATCTGAGCAGCTGCCGCGACTTCGCAATTCTAGCTCAGGAGACCGAGCCGCTGCTGGACGGCTCAGATCAGATCTTGGCGGTCGTTGATGTGCTCTTAGCCGCCGACAAACGCGTCAACAACCACCCTGACTGGTACGCTGTTCTCCAGTCCGATCGCCGATCCGATGACTTCGAACTCGTCAAGAAATCTTACCGACGGCTGGCGTTGCTCCTCCACCCGGACAAGAACAAGTACGCCTATGCGGAGCACGCGTTCAAGCTCGTCGCCGATGCATGGGCCGTTCTGTCCGATCCGGCCAAGAAGCAAATTTACGACAACGAGCTGGGGCCGTTCAGCCGGATCGATCTCGGCGGGTCGAATTCGAACAAATTGCCGGTGCGGCGGGACAGCCGGGGCCGAAACGACGCCGGTGTGGGAAACGACGGCCAGCAGCAGCCGAGGTCGAGATTGTCGACTTTCTGGACGACGTGCCCGTACTGTTACGTGCTGTACGAGTACCCTAGGGTTTACGAGAGCTGCTGTTTAAGGTGTCAGAATTGTAAGCGAGGGTTCGAGGCGGTGGTTGTTCCGAATTTGCCGCCAATGGTGCAGGGACAAGACGCTTACTACTGCTGTTGGGGGTTTTTTCCAATGGGATTTGTTGGCGGCAGCGGCGCGAAGGGTAAGTCCACCGCCGCAGCCACCGCCGCCTTTCCCAACTGGATGCCACCTGTCTTTACAACCCCGCAGAGTAAAACTCCGGTGATTGCAACGACAGTGCCTACCTCGGTGCCCGTGGTGCCGAATTCGGGGGGAGTGGTGGATATTTCGGATGGGACCCCGGAATTGAGGCCTAATCCGACTCAGAAGAAGAGAGGACGACCCAAGAAGTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001671 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008047 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0019538 GO:0019941 GO:0030163 GO:0030234 GO:0030433 GO:0031984 GO:0032781 GO:0033554 GO:0034976 GO:0036503 GO:0042175 GO:0042221 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043462 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050790 GO:0050896 GO:0051336 GO:0051345 GO:0051603 GO:0051716 GO:0060589 GO:0060590 GO:0065007 GO:0065009 GO:0071704 GO:0098772 GO:0098827 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaJ

Protein Analysis

315

Amino Acids

34.88

Weight (kDa)

8.76

Isoelectric Point (pI)

52.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	66 - 128	2.9e-17	DnaJ domain
Zn_ribbon_20	PF23551	176 - 210	7.2e-07	Zinc beta-ribbon domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011428)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01710 AT2G01710
fragaria_vesca	FvH4_5g35420
malus_domestica	MD08G1196700.v1.1 MD15G1301600.v1.1 MD15G1384400.v1.1
prunus_persica	Prupe.1G531000_v2.0.a1
pyrus_communis	pycom08g16900 pycom15g34460
rosa_chinensis	RchiOBHm_Chr7g0236821
rosa_laevigata	RLG00000001059
rosa_multiflora	Rmu_sc0002867.1_g000022 Rmu_sc0008851.1_g000010
rosa_roxburghii	Rroxscaffold_3G00224800
rosa_rugosa	Rorug07G0299600
rosa_samantha	Rh7AG454800 Rh7BG426000 Rh7CG473400 Rh7DG442700
rosa_wichuraiana	Rw7G037750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	179
AccB1I	GGYRCC	2 cut(s)	838, 847
AccI	GTMKAC	1 cut(s)	521
AccII	CGCG	4 cut(s)	84, 180, 320, 725
AclWI	GGATC	5 cut(s)	12, 75, 222, 353, 416
AcoI	YGGCCR	2 cut(s)	363, 493
AcsI	RAATTY	4 cut(s)	375, 427, 640, 853
AcyI	GRCGYC	1 cut(s)	474
AfaI	GTAC	6 cut(s)	203, 302, 545, 560, 566, 941
AfiI	CCNNNNNNNGG	4 cut(s)	268, 286, 342, 857
AflIII	ACRYGT	2 cut(s)	178, 318
AgsI	TTSAA	1 cut(s)	325
AjiI	CACGTC	1 cut(s)	537
AloI	GAACNNNNNNTCC	2 cut(s)	305, 337
AluBI	AGCT	6 cut(s)	52, 78, 101, 328, 391, 585
AluI	AGCT	6 cut(s)	52, 78, 101, 328, 391, 585
Alw21I	GWGCWC	2 cut(s)	162, 318
Alw26I	GTCTC	1 cut(s)	101
AlwI	GGATC	5 cut(s)	12, 75, 222, 353, 416
AoxI	GGCC	6 cut(s)	345, 363, 395, 463, 493, 899
ApeKI	GCWGC	8 cut(s)	75, 78, 118, 499, 502, 585, 717, 746
ApoI	RAATTY	4 cut(s)	375, 427, 640, 853
ArsI	GACNNNNNNTTYG	2 cut(s)	719, 751
AspA2I	CCTAGG	1 cut(s)	569
AspLEI	GCGC	1 cut(s)	725
AspS9I	GGNCC	4 cut(s)	345, 395, 463, 885
AsuC2I	CCSGG	4 cut(s)	65, 287, 461, 890
AsuHPI	GGTGA	1 cut(s)	823
AsuII	TTCGAA	2 cut(s)	240, 431
AvaII	GGWCC	1 cut(s)	885
AvrII	CCTAGG	1 cut(s)	569
BaeGI	GKGCMC	2 cut(s)	542, 843
BamHI	GGATCC	1 cut(s)	4
BanI	GGYRCC	2 cut(s)	838, 847
BarI	GAAGNNNNNNTAC	2 cut(s)	362, 394
Bbv12I	GWGCWC	2 cut(s)	162, 318
BbvI	GCAGC	8 cut(s)	65, 87, 105, 511, 514, 572, 729, 758
BccI	CCATC	1 cut(s)	875
BceAI	ACGGC	5 cut(s)	142, 281, 332, 382, 508
BcgI	CGANNNNNNTGC	2 cut(s)	421, 455
BcnI	CCSGG	4 cut(s)	65, 287, 461, 890
BcoDI	GTCTC	1 cut(s)	101
BfaI	CTAG	2 cut(s)	98, 570
BfmI	CTRYAG	1 cut(s)	56
BglI	GCCNNNNNGGC	1 cut(s)	448
BglII	AGATCT	1 cut(s)	138
BlnI	CCTAGG	1 cut(s)	569
BmcAI	AGTACT	1 cut(s)	941
Bme1390I	CCNGG	4 cut(s)	65, 287, 461, 890
Bme18I	GGWCC	1 cut(s)	885
BmgBI	CACGTC	1 cut(s)	537
BmgT120I	GGNCC	4 cut(s)	345, 395, 463, 885
BmiI	GGNNCC	7 cut(s)	6, 396, 464, 840, 849, 886, 887
BmrFI	CCNGG	4 cut(s)	65, 287, 461, 890
BmsI	GCATC	2 cut(s)	328, 762
BplI	GAGNNNNNCTC	2 cut(s)	790, 822
BpmI	CTGGAG	1 cut(s)	197
Bpu10I	CCTNAGC	1 cut(s)	102
Bpu14I	TTCGAA	2 cut(s)	240, 431
BpuMI	CCSGG	4 cut(s)	65, 287, 461, 890
Bsa29I	ATCGAT	1 cut(s)	411
BsaAI	YACGTR	1 cut(s)	553
BsaHI	GRCGYC	1 cut(s)	474
BsaI	GGTCTC	1 cut(s)	101
BsaJI	CCNNGG	6 cut(s)	460, 506, 569, 834, 843, 888
BsaWI	WCCGGW	1 cut(s)	808
BsaXI	ACNNNNNCTCC	2 cut(s)	305, 335
Bsc4I	CCNNNNNNNGG	4 cut(s)	268, 286, 342, 857
Bse118I	RCCGGY	2 cut(s)	442, 476
Bse1I	ACTGG	3 cut(s)	203, 214, 773
BseCI	ATCGAT	1 cut(s)	411
BseDI	CCNNGG	6 cut(s)	460, 506, 569, 834, 843, 888
BseGI	GGATG	2 cut(s)	777, 886
BseLI	CCNNNNNNNGG	4 cut(s)	268, 286, 342, 857
BseMII	CTCAG	4 cut(s)	62, 116, 144, 926
BseNI	ACTGG	3 cut(s)	203, 214, 773
BseRI	GAGGAG	1 cut(s)	269
BseSI	GKGCMC	2 cut(s)	542, 843
BseXI	GCAGC	8 cut(s)	65, 87, 105, 511, 514, 572, 729, 758
BseYI	CCCAGC	1 cut(s)	391
BsgI	GTGCAG	1 cut(s)	677
Bsh1236I	CGCG	4 cut(s)	84, 180, 320, 725
Bsh1285I	CGRYCG	2 cut(s)	150, 223
BshFI	GGCC	6 cut(s)	347, 365, 397, 465, 495, 901
BshNI	GGYRCC	2 cut(s)	838, 847
BshVI	ATCGAT	1 cut(s)	411
BsiEI	CGRYCG	2 cut(s)	150, 223
BsiHKAI	GWGCWC	2 cut(s)	162, 318
BsiSI	CCGG	9 cut(s)	64, 287, 362, 406, 443, 460, 477, 809, 890
BslFI	GGGAC	3 cut(s)	467, 675, 898
BslI	CCNNNNNNNGG	4 cut(s)	268, 286, 342, 857
BsmAI	GTCTC	1 cut(s)	101
BsmFI	GGGAC	3 cut(s)	467, 675, 898
BsnI	GGCC	6 cut(s)	347, 365, 397, 465, 495, 901
Bso31I	GGTCTC	1 cut(s)	101
Bsp119I	TTCGAA	2 cut(s)	240, 431
Bsp1286I	GDGCHC	4 cut(s)	162, 318, 542, 843
Bsp143I	GATC	9 cut(s)	4, 67, 133, 138, 220, 227, 358, 408, 412
BspANI	GGCC	6 cut(s)	347, 365, 397, 465, 495, 901
BspCNI	CTCAG	4 cut(s)	63, 115, 143, 925
BspDI	ATCGAT	1 cut(s)	411
BspFNI	CGCG	4 cut(s)	84, 180, 320, 725
BspLI	GGNNCC	7 cut(s)	6, 396, 464, 840, 849, 886, 887
BspPI	GGATC	5 cut(s)	12, 75, 222, 353, 416
BspT104I	TTCGAA	2 cut(s)	240, 431
BspT107I	GGYRCC	2 cut(s)	838, 847
BspTNI	GGTCTC	1 cut(s)	101
BsrFI	RCCGGY	2 cut(s)	442, 476
BsrI	ACTGG	3 cut(s)	203, 214, 773
BssAI	RCCGGY	2 cut(s)	442, 476
BssECI	CCNNGG	6 cut(s)	460, 506, 569, 834, 843, 888
BssMI	GATC	9 cut(s)	4, 67, 133, 138, 220, 227, 358, 408, 412
BssNI	GRCGYC	1 cut(s)	474
BssT1I	CCWWGG	1 cut(s)	569
Bst4CI	ACNGT	2 cut(s)	548, 826
Bst6I	CTCTTC	1 cut(s)	914
BstACI	GRCGYC	1 cut(s)	474
BstBAI	YACGTR	1 cut(s)	553
BstBI	TTCGAA	2 cut(s)	240, 431
BstC8I	GCNNGC	3 cut(s)	270, 318, 497
BstDEI	CTNAG	5 cut(s)	71, 102, 130, 163, 912
BstDSI	CCRYGG	1 cut(s)	843
BstF5I	GGATG	2 cut(s)	777, 886
BstFNI	CGCG	4 cut(s)	84, 180, 320, 725
BstHHI	GCGC	1 cut(s)	725
BstKTI	GATC	9 cut(s)	7, 70, 136, 141, 223, 230, 361, 411, 415
BstMAI	GTCTC	1 cut(s)	101
BstMBI	GATC	9 cut(s)	4, 67, 133, 138, 220, 227, 358, 408, 412
BstMCI	CGRYCG	2 cut(s)	150, 223
BstSCI	CCNGG	4 cut(s)	63, 285, 459, 888
BstSFI	CTRYAG	1 cut(s)	56
BstSLI	GKGCMC	2 cut(s)	542, 843
BstUI	CGCG	4 cut(s)	84, 180, 320, 725
BstV1I	GCAGC	8 cut(s)	65, 87, 105, 511, 514, 572, 729, 758
BstX2I	RGATCY	3 cut(s)	4, 67, 138
BstYI	RGATCY	3 cut(s)	4, 67, 138
Bsu15I	ATCGAT	1 cut(s)	411
BsuRI	GGCC	6 cut(s)	347, 365, 397, 465, 495, 901
BsuTUI	ATCGAT	1 cut(s)	411
BtgI	CCRYGG	1 cut(s)	843
BtrI	CACGTC	1 cut(s)	537
BtsCI	GGATG	2 cut(s)	777, 886
BtsIMutI	CAGTG	1 cut(s)	831
Cac8I	GCNNGC	3 cut(s)	270, 318, 497
CfoI	GCGC	1 cut(s)	725
Cfr10I	RCCGGY	2 cut(s)	442, 476
Cfr13I	GGNCC	4 cut(s)	345, 395, 463, 885
ClaI	ATCGAT	1 cut(s)	411
CseI	GACGC	3 cut(s)	169, 482, 677
Csp6I	GTAC	6 cut(s)	202, 301, 544, 559, 565, 940
CspCI	CAANNNNNGTGG	1 cut(s)	32
CviAII	CATG	1 cut(s)	342
CviQI	GTAC	6 cut(s)	202, 301, 544, 559, 565, 940
DdeI	CTNAG	5 cut(s)	71, 102, 130, 163, 912
DpnI	GATC	9 cut(s)	6, 69, 135, 140, 222, 229, 360, 410, 414
DpnII	GATC	9 cut(s)	4, 67, 133, 138, 220, 227, 358, 408, 412
DrdI	GACNNNNNNGTC	1 cut(s)	179
DseDI	GACNNNNNNGTC	1 cut(s)	179
EaeI	YGGCCR	2 cut(s)	363, 493
Eam1104I	CTCTTC	1 cut(s)	914
EarI	CTCTTC	1 cut(s)	914
Eco130I	CCWWGG	1 cut(s)	569
Eco147I	AGGCCT	1 cut(s)	901
Eco31I	GGTCTC	1 cut(s)	101
Eco47I	GGWCC	1 cut(s)	885
EcoO109I	RGGNCCY	1 cut(s)	885
EcoRI	GAATTC	2 cut(s)	427, 853
EcoT14I	CCWWGG	1 cut(s)	569
EcoT22I	ATGCAT	1 cut(s)	343
ErhI	CCWWGG	1 cut(s)	569
FaeI	CATG	1 cut(s)	345
FaiI	YATR	2 cut(s)	309, 343
FaqI	GGGAC	3 cut(s)	467, 675, 898
FatI	CATG	1 cut(s)	341
FauI	CCCGC	3 cut(s)	413, 444, 801
FblI	GTMKAC	1 cut(s)	521
FokI	GGATG	2 cut(s)	784, 893
FspBI	CTAG	2 cut(s)	98, 570
GlaI	GCGC	1 cut(s)	724
GsaI	CCCAGC	1 cut(s)	395
GsuI	CTGGAG	1 cut(s)	197
HaeIII	GGCC	6 cut(s)	347, 365, 397, 465, 495, 901
HapII	CCGG	9 cut(s)	64, 287, 362, 406, 443, 460, 477, 809, 890
HgaI	GACGC	3 cut(s)	169, 482, 677
HhaI	GCGC	1 cut(s)	725
Hin1I	GRCGYC	1 cut(s)	474
Hin1II	CATG	1 cut(s)	345
Hin6I	GCGC	1 cut(s)	723
HinP1I	GCGC	1 cut(s)	723
HincII	GTYRAC	2 cut(s)	184, 522
HindII	GTYRAC	2 cut(s)	184, 522
HindIII	AAGCTT	1 cut(s)	50
HinfI	GANTC	2 cut(s)	39, 910
HpaII	CCGG	9 cut(s)	64, 287, 362, 406, 443, 460, 477, 809, 890
HphI	GGTGA	1 cut(s)	823
Hpy166II	GTNNAC	4 cut(s)	184, 522, 577, 738
Hpy188III	TCNNGA	4 cut(s)	104, 250, 513, 529
Hpy8I	GTNNAC	4 cut(s)	184, 522, 577, 738
Hpy99I	CGWCG	5 cut(s)	267, 335, 476, 494, 538
HpyAV	CCTTC	1 cut(s)	721
HpyCH4III	ACNGT	2 cut(s)	548, 826
HpyCH4IV	ACGT	2 cut(s)	536, 552
HpyCH4V	TGCA	3 cut(s)	341, 658, 818
HpyF3I	CTNAG	5 cut(s)	71, 102, 130, 163, 912
HpySE526I	ACGT	2 cut(s)	536, 552
Hsp92I	GRCGYC	1 cut(s)	474
Hsp92II	CATG	1 cut(s)	345
HspAI	GCGC	1 cut(s)	723
KflI	GGGWCCC	1 cut(s)	885
Kzo9I	GATC	9 cut(s)	4, 67, 133, 138, 220, 227, 358, 408, 412
LmnI	GCTCC	2 cut(s)	282, 313
Lsp1109I	GCAGC	8 cut(s)	65, 87, 105, 511, 514, 572, 729, 758
LweI	GCATC	2 cut(s)	328, 762
MaeI	CTAG	2 cut(s)	98, 570
MaeII	ACGT	2 cut(s)	536, 552
MaeIII	GTNAC	1 cut(s)	548
MalI	GATC	9 cut(s)	6, 69, 135, 140, 222, 229, 360, 410, 414
MboI	GATC	9 cut(s)	4, 67, 133, 138, 220, 227, 358, 408, 412
MboII	GAAGA	2 cut(s)	928, 931
MflI	RGATCY	3 cut(s)	4, 67, 138
MhlI	GDGCHC	4 cut(s)	162, 318, 542, 843
MluCI	AATT	8 cut(s)	93, 375, 427, 437, 604, 640, 853, 893
MluI	ACGCGT	2 cut(s)	178, 318
MlyI	GAGTC	1 cut(s)	904
MmeI	TCCRAC	1 cut(s)	932
MnlI	CCTC	7 cut(s)	290, 501, 608, 616, 844, 891, 917
Mph1103I	ATGCAT	1 cut(s)	343
MseI	TTAA	1 cut(s)	593
MspA1I	CMGCKG	3 cut(s)	78, 118, 720
MspI	CCGG	9 cut(s)	64, 287, 362, 406, 443, 460, 477, 809, 890
MspR9I	CCNGG	4 cut(s)	65, 287, 461, 890
MvnI	CGCG	4 cut(s)	84, 180, 320, 725
NciI	CCSGG	4 cut(s)	65, 287, 461, 890
NdeII	GATC	9 cut(s)	4, 67, 133, 138, 220, 227, 358, 408, 412
NlaIII	CATG	1 cut(s)	345
NlaIV	GGNNCC	7 cut(s)	6, 396, 464, 840, 849, 886, 887
NmeAIII	GCCGAG	4 cut(s)	49, 70, 396, 531
NsiI	ATGCAT	1 cut(s)	343
NspV	TTCGAA	2 cut(s)	240, 431
PceI	AGGCCT	1 cut(s)	901
PcsI	WCGNNNNNNNCGW	4 cut(s)	42, 518, 539, 558
PfeI	GAWTC	1 cut(s)	39
Ple19I	CGATCG	1 cut(s)	223
PleI	GAGTC	1 cut(s)	904
PpsI	GAGTC	1 cut(s)	904
Ppu21I	YACGTR	1 cut(s)	553
PpuMI	RGGWCCY	1 cut(s)	885
Psp5II	RGGWCCY	1 cut(s)	885
PspFI	CCCAGC	1 cut(s)	391
PspN4I	GGNNCC	7 cut(s)	6, 396, 464, 840, 849, 886, 887
PspPI	GGNCC	4 cut(s)	345, 395, 463, 885
PspPPI	RGGWCCY	1 cut(s)	885
PsuI	RGATCY	3 cut(s)	4, 67, 138
PvuI	CGATCG	1 cut(s)	223
PvuII	CAGCTG	1 cut(s)	78
RsaI	GTAC	6 cut(s)	203, 302, 545, 560, 566, 941
RsaNI	GTAC	6 cut(s)	202, 301, 544, 559, 565, 940
SalI	GTCGAC	1 cut(s)	520
SaqAI	TTAA	1 cut(s)	593
Sau3AI	GATC	9 cut(s)	4, 67, 133, 138, 220, 227, 358, 408, 412
Sau96I	GGNCC	4 cut(s)	345, 395, 463, 885
ScaI	AGTACT	1 cut(s)	941
SchI	GAGTC	1 cut(s)	904
ScrFI	CCNGG	4 cut(s)	65, 287, 461, 890
SduI	GDGCHC	4 cut(s)	162, 318, 542, 843
SfaNI	GCATC	2 cut(s)	328, 762
SfcI	CTRYAG	1 cut(s)	56
SfuI	TTCGAA	2 cut(s)	240, 431
SgrAI	CRCCGGYG	1 cut(s)	476
SinI	GGWCC	1 cut(s)	885
Sse9I	AATT	8 cut(s)	93, 375, 427, 437, 604, 640, 853, 893
SseBI	AGGCCT	1 cut(s)	901
SspMI	CTAG	2 cut(s)	98, 570
StuI	AGGCCT	1 cut(s)	901
StyD4I	CCNGG	4 cut(s)	63, 285, 459, 888
StyI	CCWWGG	1 cut(s)	569
TaaI	ACNGT	2 cut(s)	548, 826
TaiI	ACGT	2 cut(s)	539, 555
TaqI	TCGA	7 cut(s)	240, 411, 425, 431, 512, 521, 621
TaqII	GACCGA	1 cut(s)	125
TasI	AATT	8 cut(s)	93, 375, 427, 437, 604, 640, 853, 893
TatI	WGTACW	1 cut(s)	939
TauI	GCSGC	9 cut(s)	84, 118, 170, 451, 649, 717, 723, 746, 758
TfiI	GAWTC	1 cut(s)	39
Tru1I	TTAA	1 cut(s)	593
Tru9I	TTAA	1 cut(s)	593
TscAI	CASTG	1 cut(s)	831
TseI	GCWGC	8 cut(s)	75, 78, 118, 499, 502, 585, 717, 746
TspRI	CASTG	1 cut(s)	831
VpaK11BI	GGWCC	1 cut(s)	885
XapI	RAATTY	4 cut(s)	375, 427, 640, 853
XmaJI	CCTAGG	1 cut(s)	569
XmiI	GTMKAC	1 cut(s)	521
XspI	CTAG	2 cut(s)	98, 570
ZrmI	AGTACT	1 cut(s)	941
Zsp2I	ATGCAT	1 cut(s)	343

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.