Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0011428

Physical Location & Seq

Genomic Coordinates Forward (+)

57252736 .. 57254297

Gene Structure 1562 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G037750.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1005 bp

ATGGACCACAATCCAAACAGAGCCGAAGCCGAGCGGTTGCTCGGAATCGCCGAGAAGCTCCTACAGAACCGAGATCTGAGCAGCTCGCGCGACTTCGCCATTCTGGCGCAAGAAACAGAGCCGCTATTGGACGGCTCCGATCAGATCTTAGCCGTTGTCGACGTCCTCCTAGCCGCCGATAAGCGCGTAAACAACCACCACGACTGGTACTCCATTCTCCAGGTGGATCGCCGATCCGACGACCACGATCTGATCAAGAGATCGTACCGACGGCTGGCGTTGCTCCTCCACCCGGACAAGAACAAATACGCCTTCGCCGAGCACGCCTTCAAGCTCGTCGCCGATGCATGGGCCGTTCTCTCCGACCCGGCCAAGAAGCCGGTCTTCGACACCGAGCTGGGCCTCTACAGCCCAGTCGATCTCACCGCCGCCGCAGCAGCAGCCTCCAATCCGAACAAGCTTCCGGTGCGGCGGGGGCAGAATGCTCGAAACGACGGCGTTTCGGACGAGCAGCAGCAAAATCAACAGAGGTCGAGATTATCGAGCTTCTGGACGGCGTGTCCTTACTGTTATATACTGTATGAGTACCCTAGGGTTTATGAGAATTGCTGTTTGAGGTGTCAGAATTGTAAGAGAGGCTTTGAGGCCGTTTTGGTCCCCAATTTGCCGCCATTGGTGCAAGGCCAGGATGCCTATTACTGCTGCTGGGGCTTCTTCCCCATGGGCTTTGTTGGTGGCAGCTCAGCCAATGGAGGAAAGGGAAAGGCGGCTGCCACCGCGCCGGCCTTCCCCTCTTGGATGCCTCCGATTTTCACAACGCCGCCGCCGCCACAGAATAAAACCCCTGAGACTTCCAGGGCACCCCCTGTGAATGTGCCGCCCCCACCTGCCCCCGTCCCCGCGGCGGCAACGGGCGATGTAATGTTGAATTTTTCGGGTGGGAACTCGAATTCAGGGACAGGGACTAATCCAAAGAAGAGGGGACGGCCGCGGAAGTTAACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001671 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008047 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0019538 GO:0019941 GO:0030163 GO:0030234 GO:0030433 GO:0031984 GO:0032781 GO:0033554 GO:0034976 GO:0036503 GO:0042175 GO:0042221 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043462 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050790 GO:0050896 GO:0051336 GO:0051345 GO:0051603 GO:0051716 GO:0060589 GO:0060590 GO:0065007 GO:0065009 GO:0071704 GO:0098772 GO:0098827 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaJ

Protein Analysis

334

Amino Acids

36.73

Weight (kDa)

8.71

Isoelectric Point (pI)

53.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	68 - 130	5.9e-18	DnaJ domain
Zn_ribbon_20	PF23551	183 - 217	3.5e-08	Zinc beta-ribbon domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011428)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01710 AT2G01710
fragaria_vesca	FvH4_5g35420
malus_domestica	MD08G1196700.v1.1 MD15G1301600.v1.1 MD15G1384400.v1.1
prunus_persica	Prupe.1G531000_v2.0.a1
pyrus_communis	pycom08g16900 pycom15g34460
rosa_chinensis	RchiOBHm_Chr7g0236821
rosa_laevigata	RLG00000001059
rosa_multiflora	Rmu_sc0002867.1_g000022 Rmu_sc0008851.1_g000010
rosa_roxburghii	Rroxscaffold_3G00224800
rosa_rugosa	Rorug07G0299600
rosa_samantha	Rh7AG454800 Rh7BG426000 Rh7CG473400 Rh7DG442700
rosa_wichuraiana	Rw7G037750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	895
AatII	GACGTC	1 cut(s)	165
Acc36I	ACCTGC	1 cut(s)	895
AccB1I	GGYRCC	1 cut(s)	859
AccBSI	CCGCTC	1 cut(s)	34
AccI	GTMKAC	1 cut(s)	159
AccII	CGCG	6 cut(s)	88, 90, 186, 779, 902, 991
AclWI	GGATC	2 cut(s)	228, 234
AcoI	YGGCCR	2 cut(s)	369, 986
AcsI	RAATTY	2 cut(s)	928, 949
AcyI	GRCGYC	1 cut(s)	162
AfaI	GTAC	3 cut(s)	209, 266, 587
AfiI	CCNNNNNNNGG	4 cut(s)	274, 292, 348, 904
AgsI	TTSAA	2 cut(s)	331, 928
AhdI	GACNNNNNGTC	1 cut(s)	558
AjnI	CCWGG	3 cut(s)	219, 684, 854
AluBI	AGCT	7 cut(s)	58, 84, 334, 397, 460, 546, 741
AluI	AGCT	7 cut(s)	58, 84, 334, 397, 460, 546, 741
Alw21I	GWGCWC	1 cut(s)	324
Alw26I	GTCTC	1 cut(s)	842
AlwI	GGATC	2 cut(s)	228, 234
AoxI	GGCC	7 cut(s)	351, 369, 400, 645, 682, 783, 986
ApeKI	GCWGC	9 cut(s)	81, 434, 437, 440, 511, 514, 702, 738, 770
ApoI	RAATTY	2 cut(s)	928, 949
AspA2I	CCTAGG	1 cut(s)	590
AspLEI	GCGC	4 cut(s)	90, 109, 186, 781
AspS9I	GGNCC	4 cut(s)	4, 351, 400, 655
AsuC2I	CCSGG	2 cut(s)	293, 368
AsuHPI	GGTGA	1 cut(s)	415
AvaII	GGWCC	2 cut(s)	4, 655
AvrII	CCTAGG	1 cut(s)	590
BaeGI	GKGCMC	1 cut(s)	862
BanI	GGYRCC	1 cut(s)	859
BbsI	GAAGAC	1 cut(s)	376
Bbv12I	GWGCWC	1 cut(s)	324
BbvI	GCAGC	9 cut(s)	93, 446, 449, 452, 523, 526, 689, 750, 757
BceAI	ACGGC	8 cut(s)	137, 148, 287, 338, 511, 570, 632, 1001
BcgI	CGANNNNNNTGC	2 cut(s)	467, 501
BciT130I	CCWGG	3 cut(s)	221, 686, 856
BclI	TGATCA	1 cut(s)	252
BcnI	CCSGG	2 cut(s)	293, 368
BcoDI	GTCTC	1 cut(s)	842
BfaI	CTAG	2 cut(s)	170, 591
BfmI	CTRYAG	2 cut(s)	62, 406
BfuAI	ACCTGC	1 cut(s)	895
BglI	GCCNNNNNGGC	1 cut(s)	104
BglII	AGATCT	2 cut(s)	73, 144
BlnI	CCTAGG	1 cut(s)	590
BlpI	GCTNAGC	1 cut(s)	742
Bme1390I	CCNGG	5 cut(s)	221, 293, 368, 686, 856
Bme18I	GGWCC	2 cut(s)	4, 655
BmeRI	GACNNNNNGTC	1 cut(s)	558
BmgT120I	GGNCC	4 cut(s)	4, 351, 400, 655
BmiI	GGNNCC	3 cut(s)	136, 657, 861
BmrFI	CCNGG	5 cut(s)	221, 293, 368, 686, 856
BmrI	ACTGGG	1 cut(s)	407
BmsI	GCATC	3 cut(s)	334, 679, 789
BmuI	ACTGGG	1 cut(s)	407
BpiI	GAAGAC	1 cut(s)	376
BpmI	CTGGAG	1 cut(s)	203
Bpu1102I	GCTNAGC	1 cut(s)	742
BpuMI	CCSGG	2 cut(s)	293, 368
BsaHI	GRCGYC	1 cut(s)	162
BsaJI	CCNNGG	5 cut(s)	590, 720, 855, 900, 989
BsaWI	WCCGGW	1 cut(s)	463
BsaXI	ACNNNNNCTCC	2 cut(s)	194, 224
Bsc4I	CCNNNNNNNGG	4 cut(s)	274, 292, 348, 904
Bse118I	RCCGGY	2 cut(s)	379, 781
Bse1I	ACTGG	2 cut(s)	209, 413
BseBI	CCWGG	3 cut(s)	221, 686, 856
BseDI	CCNNGG	5 cut(s)	590, 720, 855, 900, 989
BseGI	GGATG	2 cut(s)	694, 804
BseLI	CCNNNNNNNGG	4 cut(s)	274, 292, 348, 904
BseMII	CTCAG	3 cut(s)	68, 756, 837
BseNI	ACTGG	2 cut(s)	209, 413
BseRI	GAGGAG	1 cut(s)	275
BseSI	GKGCMC	1 cut(s)	862
BseX3I	CGGCCG	1 cut(s)	986
BseXI	GCAGC	9 cut(s)	93, 446, 449, 452, 523, 526, 689, 750, 757
BseYI	CCCAGC	2 cut(s)	397, 705
Bsh1236I	CGCG	6 cut(s)	88, 90, 186, 779, 902, 991
Bsh1285I	CGRYCG	1 cut(s)	989
BshFI	GGCC	7 cut(s)	353, 371, 402, 647, 684, 785, 988
BshNI	GGYRCC	1 cut(s)	859
BsiEI	CGRYCG	1 cut(s)	989
BsiHKAI	GWGCWC	1 cut(s)	324
BsiSI	CCGG	5 cut(s)	293, 368, 380, 464, 782
BslFI	GGGAC	5 cut(s)	641, 881, 970, 976, 996
BslI	CCNNNNNNNGG	4 cut(s)	274, 292, 348, 904
BsmAI	GTCTC	1 cut(s)	842
BsmFI	GGGAC	5 cut(s)	641, 881, 970, 976, 996
BsmI	GAATGC	1 cut(s)	487
BsnI	GGCC	7 cut(s)	353, 371, 402, 647, 684, 785, 988
Bsp1286I	GDGCHC	2 cut(s)	324, 862
Bsp143I	GATC	9 cut(s)	73, 139, 144, 226, 233, 247, 252, 260, 418
Bsp1720I	GCTNAGC	1 cut(s)	742
Bsp19I	CCATGG	1 cut(s)	720
BspANI	GGCC	7 cut(s)	353, 371, 402, 647, 684, 785, 988
BspCNI	CTCAG	3 cut(s)	69, 755, 838
BspFNI	CGCG	6 cut(s)	88, 90, 186, 779, 902, 991
BspLI	GGNNCC	3 cut(s)	136, 657, 861
BspMI	ACCTGC	1 cut(s)	895
BspPI	GGATC	2 cut(s)	228, 234
BspT107I	GGYRCC	1 cut(s)	859
BsrBI	CCGCTC	1 cut(s)	34
BsrFI	RCCGGY	2 cut(s)	379, 781
BsrI	ACTGG	2 cut(s)	209, 413
BssAI	RCCGGY	2 cut(s)	379, 781
BssECI	CCNNGG	5 cut(s)	590, 720, 855, 900, 989
BssMI	GATC	9 cut(s)	73, 139, 144, 226, 233, 247, 252, 260, 418
BssNI	GRCGYC	1 cut(s)	162
BssT1I	CCWWGG	2 cut(s)	590, 720
Bst2UI	CCWGG	3 cut(s)	221, 686, 856
Bst4CI	ACNGT	2 cut(s)	569, 579
Bst6I	CTCTTC	1 cut(s)	971
BstACI	GRCGYC	1 cut(s)	162
BstC8I	GCNNGC	4 cut(s)	86, 276, 324, 783
BstDEI	CTNAG	5 cut(s)	77, 148, 742, 846, 1002
BstDSI	CCRYGG	3 cut(s)	720, 900, 989
BstF5I	GGATG	2 cut(s)	694, 804
BstFNI	CGCG	6 cut(s)	88, 90, 186, 779, 902, 991
BstHHI	GCGC	4 cut(s)	90, 109, 186, 781
BstKTI	GATC	9 cut(s)	76, 142, 147, 229, 236, 250, 255, 263, 421
BstMAI	GTCTC	1 cut(s)	842
BstMBI	GATC	9 cut(s)	73, 139, 144, 226, 233, 247, 252, 260, 418
BstMCI	CGRYCG	1 cut(s)	989
BstNI	CCWGG	3 cut(s)	221, 686, 856
BstSCI	CCNGG	5 cut(s)	219, 291, 366, 684, 854
BstSFI	CTRYAG	2 cut(s)	62, 406
BstSLI	GKGCMC	1 cut(s)	862
BstUI	CGCG	6 cut(s)	88, 90, 186, 779, 902, 991
BstV1I	GCAGC	9 cut(s)	93, 446, 449, 452, 523, 526, 689, 750, 757
BstV2I	GAAGAC	1 cut(s)	376
BstX2I	RGATCY	2 cut(s)	73, 144
BstYI	RGATCY	2 cut(s)	73, 144
BstZI	CGGCCG	1 cut(s)	986
BsuRI	GGCC	7 cut(s)	353, 371, 402, 647, 684, 785, 988
BtgI	CCRYGG	3 cut(s)	720, 900, 989
BtgZI	GCGATG	1 cut(s)	930
BtsCI	GGATG	2 cut(s)	694, 804
BveI	ACCTGC	1 cut(s)	895
Cac8I	GCNNGC	4 cut(s)	86, 276, 324, 783
CfoI	GCGC	4 cut(s)	90, 109, 186, 781
Cfr10I	RCCGGY	2 cut(s)	379, 781
Cfr13I	GGNCC	4 cut(s)	4, 351, 400, 655
Cfr42I	CCGCGG	2 cut(s)	903, 992
Csp6I	GTAC	3 cut(s)	208, 265, 586
CviAII	CATG	2 cut(s)	348, 721
CviQI	GTAC	3 cut(s)	208, 265, 586
DdeI	CTNAG	5 cut(s)	77, 148, 742, 846, 1002
DpnI	GATC	9 cut(s)	75, 141, 146, 228, 235, 249, 254, 262, 420
DpnII	GATC	9 cut(s)	73, 139, 144, 226, 233, 247, 252, 260, 418
DriI	GACNNNNNGTC	1 cut(s)	558
EaeI	YGGCCR	2 cut(s)	369, 986
EagI	CGGCCG	1 cut(s)	986
Eam1104I	CTCTTC	1 cut(s)	971
Eam1105I	GACNNNNNGTC	1 cut(s)	558
EarI	CTCTTC	1 cut(s)	971
EclXI	CGGCCG	1 cut(s)	986
Eco130I	CCWWGG	2 cut(s)	590, 720
Eco47I	GGWCC	2 cut(s)	4, 655
Eco52I	CGGCCG	1 cut(s)	986
EcoRI	GAATTC	1 cut(s)	949
EcoRII	CCWGG	3 cut(s)	219, 684, 854
EcoT14I	CCWWGG	2 cut(s)	590, 720
EcoT22I	ATGCAT	1 cut(s)	349
ErhI	CCWWGG	2 cut(s)	590, 720
FaeI	CATG	2 cut(s)	351, 724
FaiI	YATR	6 cut(s)	349, 573, 575, 582, 600, 722
FalI	AAGNNNNNCTT	4 cut(s)	368, 400, 623, 655
FaqI	GGGAC	5 cut(s)	641, 881, 970, 976, 996
FatI	CATG	2 cut(s)	347, 720
FauI	CCCGC	2 cut(s)	465, 907
FbaI	TGATCA	1 cut(s)	252
FblI	GTMKAC	1 cut(s)	159
FokI	GGATG	2 cut(s)	701, 811
FspBI	CTAG	2 cut(s)	170, 591
GlaI	GCGC	4 cut(s)	89, 108, 185, 780
GsaI	CCCAGC	2 cut(s)	401, 709
GsuI	CTGGAG	1 cut(s)	203
HaeIII	GGCC	7 cut(s)	353, 371, 402, 647, 684, 785, 988
HapII	CCGG	5 cut(s)	293, 368, 380, 464, 782
HhaI	GCGC	4 cut(s)	90, 109, 186, 781
Hin1I	GRCGYC	1 cut(s)	162
Hin1II	CATG	2 cut(s)	351, 724
Hin6I	GCGC	4 cut(s)	88, 107, 184, 779
HinP1I	GCGC	4 cut(s)	88, 107, 184, 779
HincII	GTYRAC	2 cut(s)	160, 999
HindII	GTYRAC	2 cut(s)	160, 999
HindIII	AAGCTT	1 cut(s)	458
HinfI	GANTC	1 cut(s)	45
HpaI	GTTAAC	1 cut(s)	999
HpaII	CCGG	5 cut(s)	293, 368, 380, 464, 782
HphI	GGTGA	1 cut(s)	415
Hpy166II	GTNNAC	3 cut(s)	160, 190, 999
Hpy188III	TCNNGA	3 cut(s)	256, 534, 550
Hpy8I	GTNNAC	3 cut(s)	160, 190, 999
Hpy99I	CGWCG	5 cut(s)	164, 242, 273, 341, 497
HpyAV	CCTTC	3 cut(s)	322, 337, 796
HpyCH4III	ACNGT	2 cut(s)	569, 579
HpyCH4IV	ACGT	1 cut(s)	162
HpyCH4V	TGCA	2 cut(s)	347, 679
HpyF3I	CTNAG	5 cut(s)	77, 148, 742, 846, 1002
HpySE526I	ACGT	1 cut(s)	162
Hsp92I	GRCGYC	1 cut(s)	162
Hsp92II	CATG	2 cut(s)	351, 724
HspAI	GCGC	4 cut(s)	88, 107, 184, 779
KroI	GCCGGC	1 cut(s)	781
KroNI	GCCGGC	1 cut(s)	783
Ksp22I	TGATCA	1 cut(s)	252
KspAI	GTTAAC	1 cut(s)	999
KspI	CCGCGG	2 cut(s)	903, 992
Kzo9I	GATC	9 cut(s)	73, 139, 144, 226, 233, 247, 252, 260, 418
LmnI	GCTCC	3 cut(s)	63, 140, 288
Lsp1109I	GCAGC	9 cut(s)	93, 446, 449, 452, 523, 526, 689, 750, 757
LweI	GCATC	3 cut(s)	334, 679, 789
MaeI	CTAG	2 cut(s)	170, 591
MaeII	ACGT	1 cut(s)	162
MalI	GATC	9 cut(s)	75, 141, 146, 228, 235, 249, 254, 262, 420
MbiI	CCGCTC	1 cut(s)	34
MboI	GATC	9 cut(s)	73, 139, 144, 226, 233, 247, 252, 260, 418
MboII	GAAGA	3 cut(s)	376, 706, 988
MflI	RGATCY	2 cut(s)	73, 144
MhlI	GDGCHC	2 cut(s)	324, 862
MluCI	AATT	5 cut(s)	604, 625, 661, 928, 949
MmeI	TCCRAC	2 cut(s)	261, 387
Mph1103I	ATGCAT	1 cut(s)	349
MroNI	GCCGGC	1 cut(s)	781
MseI	TTAA	1 cut(s)	998
MspA1I	CMGCKG	2 cut(s)	902, 991
MspI	CCGG	5 cut(s)	293, 368, 380, 464, 782
MspR9I	CCNGG	5 cut(s)	221, 293, 368, 686, 856
Mva1269I	GAATGC	1 cut(s)	487
MvaI	CCWGG	3 cut(s)	221, 686, 856
MvnI	CGCG	6 cut(s)	88, 90, 186, 779, 902, 991
NaeI	GCCGGC	1 cut(s)	783
NciI	CCSGG	2 cut(s)	293, 368
NcoI	CCATGG	1 cut(s)	720
NdeII	GATC	9 cut(s)	73, 139, 144, 226, 233, 247, 252, 260, 418
NgoMIV	GCCGGC	1 cut(s)	781
NlaIII	CATG	2 cut(s)	351, 724
NlaIV	GGNNCC	3 cut(s)	136, 657, 861
NmeAIII	GCCGAG	3 cut(s)	55, 76, 343
NsiI	ATGCAT	1 cut(s)	349
PaqCI	CACCTGC	1 cut(s)	895
PcsI	WCGNNNNNNNCGW	3 cut(s)	48, 315, 539
PctI	GAATGC	1 cut(s)	487
PdiI	GCCGGC	1 cut(s)	783
PfeI	GAWTC	1 cut(s)	45
Psp6I	CCWGG	3 cut(s)	219, 684, 854
PspFI	CCCAGC	2 cut(s)	397, 705
PspGI	CCWGG	3 cut(s)	219, 684, 854
PspN4I	GGNNCC	3 cut(s)	136, 657, 861
PspPI	GGNCC	4 cut(s)	4, 351, 400, 655
PsuI	RGATCY	2 cut(s)	73, 144
RsaI	GTAC	3 cut(s)	209, 266, 587
RsaNI	GTAC	3 cut(s)	208, 265, 586
SacII	CCGCGG	2 cut(s)	903, 992
SalI	GTCGAC	1 cut(s)	158
SaqAI	TTAA	1 cut(s)	998
Sau3AI	GATC	9 cut(s)	73, 139, 144, 226, 233, 247, 252, 260, 418
Sau96I	GGNCC	4 cut(s)	4, 351, 400, 655
ScrFI	CCNGG	5 cut(s)	221, 293, 368, 686, 856
SduI	GDGCHC	2 cut(s)	324, 862
SfaNI	GCATC	3 cut(s)	334, 679, 789
SfcI	CTRYAG	2 cut(s)	62, 406
Sfr303I	CCGCGG	2 cut(s)	903, 992
SgrBI	CCGCGG	2 cut(s)	903, 992
SinI	GGWCC	2 cut(s)	4, 655
Sse9I	AATT	5 cut(s)	604, 625, 661, 928, 949
SspMI	CTAG	2 cut(s)	170, 591
StyD4I	CCNGG	5 cut(s)	219, 291, 366, 684, 854
StyI	CCWWGG	2 cut(s)	590, 720
TaaI	ACNGT	2 cut(s)	569, 579
TaiI	ACGT	1 cut(s)	165
TaqI	TCGA	7 cut(s)	159, 387, 417, 487, 533, 542, 947
TasI	AATT	5 cut(s)	604, 625, 661, 928, 949
TfiI	GAWTC	1 cut(s)	45
Tru1I	TTAA	1 cut(s)	998
Tru9I	TTAA	1 cut(s)	998
TseI	GCWGC	9 cut(s)	81, 434, 437, 440, 511, 514, 702, 738, 770
VpaK11BI	GGWCC	2 cut(s)	4, 655
XapI	RAATTY	2 cut(s)	928, 949
XcmI	CCANNNNNNNNNTGG	1 cut(s)	220
XmaJI	CCTAGG	1 cut(s)	590
XmiI	GTMKAC	1 cut(s)	159
XspI	CTAG	2 cut(s)	170, 591
ZraI	GACGTC	1 cut(s)	163
Zsp2I	ATGCAT	1 cut(s)	349

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.