Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0012629

Physical Location & Seq

Genomic Coordinates Reverse (-)

26262180 .. 26268423

Gene Structure 6244 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1203200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3396 bp

ATGACAACCCTAAGATCAAACTTGCCGTCGAGGATTCGGCAACTCCTTTCAGCCGACGGTCCTCTCGGTCCGTCCATCAAACTCGACTCCGAGCCACCTCCTAAGATCAAAGCCTTTATCAATAAAGTGATACAATGTCCGTTACAAGATATAGCAATACCTCTTTCCGGTTTCCATTGGGAGTACAGTAAGGGTAATTTTAACCATTGGAGGCCGCTTTTTCTGCATTTTGATACATATTTCAAGACATACTTGTCTTGTAGAAATGATCTACTGTTGTCGGATAAAATCTTAGAAGATGATAGTCCGTTTCCAAAGCATGCAGTGCTACAAATCTTGAGAGTGATGCAAACAATTTTAGAAAACTGCAATAACAAGAGTTCATTTGATGGCTTAGAGCATTTCAAGCTCCTACTCGCATCAACGGATCCCGAGGTCCTTATAGCTGCATTAGAAACTCTTTCCGCACTTGTGAAAATAAATCCCTCCAAGCTGCATGTCAGTGGGAAGATGATCGGTTGTGGTTCTGTAAACAGCTATCTCCTGTCTCTGGCACAGGGCTGGGGAAGCAAGGAAGAGGGATTGGGTTTATATTCATGTGTTATGGCAAACGAGACTAACCAAGACGACGGGCTCAATTTGTTCCCATCTGATGTAGAAAATGATTCTGACAAGTTGCAGTGCCGAATAGGTTCCACGCTCTATTTTGAATTGCATGGGAATGCCCAAAGTACAGAGGAGAGCTCTAGCAATGTAAATAATTCTTCAAGTTTGGGGGTTATAAACATTCCAGATTTGCATTTGCAAAAGGAGGATGATTTAAAGCTAATGAAACGATATATAGAAGAGCACAGGGTACCTTCTGAACTACGGTTCTCACTGCTGACAAGAATCAGATATGCTCGTGCCTTCCGTTCTCCAAAAATATGTAGGCTGTACAGCAGGATTTGCCTTCTTGCATTTGTTGTGCTCGTTCAGTCTAGTGATGCCAGTGCAGAACTGAATTCCTTCTTTGCTAATGAGCCAGAGTTTACAAATGAATTAATCAGAATTGTGCGGTCCGAAGAATCGGTGTCTGGGACCATCAGAACGCAGGCAATGCTTGCCTTGGGTGCTCAGTTAGCTGCATATTCAGCGTCTCATGAGCGGGCACGAATCTTGAGTGTATCTAGTATCAGTTTTGCAGGGGGAAATCGTATGATACTCCTGAATGTGCTACAGAGAGCTGTTAGATCATTAAAAAACTCCAATGATCCAACTTCTCTTGCTTTTGTTGAAGCACTTCTTCAGTTCTATCTGCTCCATGTGGTTTCATCATCAGCTACAGGGAGTAATATTAGAGGGTCAGGCATGGTTCCAACATTCTTGCCTCTTTTGGAGGATTCTGATCCTTTGCATTTGCATCTAGTCTGTTTTGCTGTCAAAACTCTACAAAAGCTCATGGATTACAGTAGTTCAGCTGTGTCTCTGTTTAAAGAGTCGGGGGGAGTTGAGCTTTTGTCTCAGAGACTACAGATAGAGGTACACAGAGTTATTGGTTTGGCTGGTGATAATGATAACTCAATGGTCATCGGAGAAAGCTCAAGATACAGTGACGACCAGCTGTACTCACAGAAGAGACTCATTAAAGCTTCGTTGAAGGCACTTGGGTCTGCTACATATGCCGCTGGGAATTCCTCCAGAGTGCAACACTCCCATGACAGTTCTTTACCTGCAACTCTCTCTCTGATATTTGGGAATGTGGACAAATTTGGTGGTGACATTTATTACTCTGCAGTGACTGTTTTGAGTGAAACTATACACAAAGACCCAACTTGCTTTTCTGCTTTGCATGAAATGGGTCTGCCAGATGCATTTATATCCTCAGTTGTAGCTGGAATTCTTCCCTCTGCAAAGGCTCTTACATGTGTACCTAACGGTCTTGGTGCTATTTGTCTTAATGCCAAAGGCTTGGAGGCTGTGAAGGAAGGTTCAGCATTACGCTTTCTTGTTGACATCTTCACGAGCAAGAAATATGTTACTGCAATGAATGAAGCTATTGTTCCTTTGGCAAATGCAGTGGAAGAGCTTTTGCGCCATGTATCTTCGCTGCGAAGCACTGGTGTTGATATCATCCTTGAGATAATTGATAAAATTGCTTCCTTCACTGACAGCCACAATACAGGGGCAGCAGGAAAGGCAAATGCGAGTACTGCAATGGAAATGGATTCTGAAGAGAGAGAAAATGAAGGTAGTTGCTGTCTGGTTGATTCTGTAGATTCAGCTGCAGATGGGATCGGTAATGAGCAGTTCATTCAATTATCCCTCTTTCATTTGATGATACTGGTCCACCGAACAATGGAGAATTCCGAAACATGTCGGTTGTTTGTCGAGAAGTCAGGAATTGATGCATTACTTAAGCTTCTTCTCCAACCTGCTATTGCACAATCATCCGATGGGATGTCTATTGCTTTGCACAGTACTATGGTTTTCAAGGGTTTTACTCAACATCACTCTGCTGCCCTTGCCCGTGCATTTTGCTCCTCCCTTAGGGACCACCTAAAGAAAGCTTTGTCTGGTTTTGGGGCGGTTTCAGGATCCTTTTTGCTGGAACCTAGGATGGCATCAGATGGTGGAATTTTTTCTTCTCTGTTTCTTGTTGAGTTCCTTTTGTTTATTGCTGCCTCAAAGGACAACCGCTGGGTAACTGCATTGCTTACGGAATTTGGAAATGGTAGCAAGGATGTTCTTGAAGATATAGGACATGTCCATCGTGAAGTTTTATGGCAGATTGCTCTTTTAGAAGATACAAAATTGGACTTGGAGGATGATAATGCTGGTTCAACCAGTGAGTCACAACAGTCAGAAACAAATACAAGTGAAACTGAAGAGCAAAGGTTCAACTCTTTTAGGCAGTTCCTTGATCCATTGCTCAGAAGGAGGACTTCTGGGTGGAGCATTGAATCCCAGTTTCTGGACCTTATTAGCCTATACCGCGACCTTGGTCGCGCCACTAGTTCTCAACAAAGAACAAATTATGATGGTCATTCAAATCTTCGGATTGGATCCAGTCATCAGTTCCATCATTCTGGGTCTTCTGATGTTGTTGGCCCTCTTAATAAAAAGGAGTATGACCAGCAGAGGTCATATTATACTTCTTGCTGTGACATGGTGAGATCACTTTCTTTTCACATTACCCATCTGTTTCAAGAGCTGGGGAAGGTCATGTCGGTACCTTCTCGTCGACGGGATGATATTGTGAATGTTTCTCCTGCTGCAAAATCTGTGGCTTCTACTTTTGCATCCATTGCATTTGATCACTTGAACTTCGAAGGGCATGCTAATTCTTCTGAATCCAAGGCTTCCATATCAACAAAGTGTCGGTACTTTGGTAAAGTTATTGATTTCATTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

DUF4414 DUF908 DUF913 HECT UBA

Protein Analysis

1132

Amino Acids

124.44

Weight (kDa)

5.93

Isoelectric Point (pI)

44.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF908	PF06012	107 - 167	1.4e-11	Domain of Unknown Function (DUF908)
DUF908	PF06012	241 - 410	9.7e-22	Domain of Unknown Function (DUF908)
DUF913	PF06025	473 - 806	5.9e-63	Domain of Unknown Function (DUF913)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012629)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g34290 FvH4_5g34290
malus_domestica	MD08G1203000.v1.1 MD08G1203200.v1.1 MD15G1388600.v1.1
prunus_persica	Prupe.1G535000_v2.0.a1
pyrus_communis	pycom08g17530 pycom15g34850
rosa_chinensis	RchiOBHm_Chr7g0234861
rosa_laevigata	RLG00000001209
rosa_multiflora	Rmu_sc0000039.1_g000001
rosa_roxburghii	Rroxscaffold_3G00226700
rosa_rugosa	Rorug07G0285700 Rorug07G0285800
rosa_samantha	Rh7AG440400 Rh7BG413200 Rh7CG462100 Rh7DG430100
rosa_wichuraiana	Rw7G036610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	782
AasI	GACNNNNNNGTC	1 cut(s)	253
Acc36I	ACCTGC	2 cut(s)	1720, 2422
Acc65I	GGTACC	2 cut(s)	856, 3211
AccB1I	GGYRCC	2 cut(s)	856, 3211
AccB7I	CCANNNNNTGG	1 cut(s)	2953
AccBSI	CCGCTC	1 cut(s)	1147
AccI	GTMKAC	1 cut(s)	3223
AccII	CGCG	2 cut(s)	2976, 2988
AciI	CCGC	8 cut(s)	215, 465, 1057, 1147, 1665, 2567, 2677, 2974
AcsI	RAATTY	7 cut(s)	1003, 1672, 1748, 1878, 2344, 2616, 2702
AcuI	CTGAAG	3 cut(s)	1271, 2232, 2886
AfiI	CCNNNNNNNGG	5 cut(s)	167, 550, 2338, 2529, 2953
AflII	CTTAAG	1 cut(s)	2396
AflIII	ACRYGT	3 cut(s)	1904, 2354, 2743
AhlI	ACTAGT	1 cut(s)	2993
AjuI	GAANNNNNNNTTGG	2 cut(s)	1804, 1836
Alw21I	GWGCWC	4 cut(s)	746, 852, 972, 1117
Alw26I	GTCTC	7 cut(s)	552, 608, 1143, 1470, 1501, 1506, 1612
AlwNI	CAGNNNCTG	2 cut(s)	1781, 2953
Ama87I	CYCGRG	1 cut(s)	431
AoxI	GGCC	2 cut(s)	212, 3088
ApeKI	GCWGC	9 cut(s)	446, 493, 1124, 2089, 2168, 2264, 2498, 2660, 3254
ApoI	RAATTY	7 cut(s)	1003, 1672, 1748, 1878, 2344, 2616, 2702
ArsI	GACNNNNNNTTYG	2 cut(s)	11, 43
AseI	ATTAAT	1 cut(s)	1043
Asp700I	GAANNNNTTC	4 cut(s)	691, 1007, 1281, 2620
Asp718I	GGTACC	2 cut(s)	856, 3211
AspA2I	CCTAGG	1 cut(s)	2594
AspLEI	GCGC	2 cut(s)	2076, 2990
AspS9I	GGNCC	9 cut(s)	59, 68, 436, 1059, 1080, 2326, 2533, 2956, 3089
AsuHPI	GGTGA	3 cut(s)	1559, 1769, 3163
AsuII	TTCGAA	1 cut(s)	3309
AvaI	CYCGRG	1 cut(s)	431
AvaII	GGWCC	8 cut(s)	59, 68, 436, 1059, 1080, 2326, 2533, 2956
AvrII	CCTAGG	1 cut(s)	2594
AxyI	CCTNAGG	1 cut(s)	2528
BaeGI	GKGCMC	1 cut(s)	1153
BaeI	ACNNNNGTAYC	4 cut(s)	1893, 1926, 3355, 3388
BamHI	GGATCC	3 cut(s)	427, 2576, 3044
BanI	GGYRCC	2 cut(s)	856, 3211
BanII	GRGCYC	2 cut(s)	636, 746
BauI	CACGAG	2 cut(s)	903, 2002
BbsI	GAAGAC	1 cut(s)	3066
Bbv12I	GWGCWC	4 cut(s)	746, 852, 972, 1117
BbvI	GCAGC	9 cut(s)	433, 480, 1111, 2076, 2180, 2251, 2485, 2647, 3241
BceAI	ACGGC	1 cut(s)	10
BcgI	CGANNNNNNTGC	2 cut(s)	3299, 3333
BclI	TGATCA	1 cut(s)	3295
BcoDI	GTCTC	7 cut(s)	552, 608, 1143, 1470, 1501, 1506, 1612
BcuI	ACTAGT	1 cut(s)	2993
BfaI	CTAG	6 cut(s)	747, 981, 1170, 1406, 2595, 2994
BfmI	CTRYAG	6 cut(s)	1217, 1323, 1511, 1773, 2253, 2265
BfrI	CTTAAG	1 cut(s)	2396
BfuAI	ACCTGC	2 cut(s)	1720, 2422
BlnI	CCTAGG	1 cut(s)	2594
BmcAI	AGTACT	2 cut(s)	2191, 2461
Bme18I	GGWCC	8 cut(s)	59, 68, 436, 1059, 1080, 2326, 2533, 2956
BmeT110I	CYCGRG	1 cut(s)	431
BmgT120I	GGNCC	9 cut(s)	59, 68, 436, 1059, 1080, 2326, 2533, 2956, 3089
BmrI	ACTGGG	1 cut(s)	2941
BmsI	GCATC	8 cut(s)	336, 428, 976, 1411, 1840, 2377, 2612, 3290
BmuI	ACTGGG	1 cut(s)	2941
BoxI	GACNNNNGTC	1 cut(s)	2982
BpiI	GAAGAC	1 cut(s)	3066
BplI	GAGNNNNNCTC	2 cut(s)	728, 760
BpmI	CTGGAG	1 cut(s)	1663
Bpu14I	TTCGAA	1 cut(s)	3309
BpuEI	CTTGAG	4 cut(s)	358, 1180, 1567, 2138
BsaBI	GATNNNNATC	1 cut(s)	1386
BsaJI	CCNNGG	5 cut(s)	432, 1107, 2594, 2980, 3336
BsaWI	WCCGGW	1 cut(s)	167
Bsc4I	CCNNNNNNNGG	5 cut(s)	167, 550, 2338, 2529, 2953
Bse1I	ACTGG	6 cut(s)	990, 2104, 2328, 2826, 2947, 3048
Bse21I	CCTNAGG	1 cut(s)	2528
Bse3DI	GCAATG	7 cut(s)	757, 1104, 2031, 2202, 2690, 2906, 3285
Bse8I	GATNNNNATC	1 cut(s)	1386
BseDI	CCNNGG	5 cut(s)	432, 1107, 2594, 2980, 3336
BseGI	GGATG	9 cut(s)	820, 2112, 2429, 2445, 2604, 2728, 2812, 3235, 3281
BseJI	GATNNNNATC	1 cut(s)	1386
BseLI	CCNNNNNNNGG	5 cut(s)	167, 550, 2338, 2529, 2953
BseMI	GCAATG	7 cut(s)	757, 1104, 2031, 2202, 2690, 2906, 3285
BseMII	CTCAG	4 cut(s)	1130, 1517, 1878, 2926
BseNI	ACTGG	6 cut(s)	990, 2104, 2328, 2826, 2947, 3048
BseRI	GAGGAG	2 cut(s)	752, 2512
BseSI	GKGCMC	1 cut(s)	1153
BseXI	GCAGC	9 cut(s)	433, 480, 1111, 2076, 2180, 2251, 2485, 2647, 3241
BseYI	CCCAGC	4 cut(s)	561, 1667, 2679, 3193
BsgI	GTGCAG	1 cut(s)	1014
Bsh1236I	CGCG	2 cut(s)	2976, 2988
BshFI	GGCC	2 cut(s)	214, 3090
BshNI	GGYRCC	2 cut(s)	856, 3211
BsiHKAI	GWGCWC	4 cut(s)	746, 852, 972, 1117
BsiHKCI	CYCGRG	1 cut(s)	431
BsiSI	CCGG	1 cut(s)	168
BslFI	GGGAC	2 cut(s)	1093, 2546
BslI	CCNNNNNNNGG	5 cut(s)	167, 550, 2338, 2529, 2953
BsmAI	GTCTC	7 cut(s)	552, 608, 1143, 1470, 1501, 1506, 1612
BsmBI	CGTCTC	1 cut(s)	1143
BsmFI	GGGAC	2 cut(s)	1093, 2546
BsmI	GAATGC	1 cut(s)	727
BsnI	GGCC	2 cut(s)	214, 3090
BsoBI	CYCGRG	1 cut(s)	431
Bsp119I	TTCGAA	1 cut(s)	3309
Bsp1286I	GDGCHC	6 cut(s)	636, 746, 852, 972, 1117, 1153
Bsp1407I	TGTACA	1 cut(s)	936
BspACI	CCGC	8 cut(s)	215, 465, 1057, 1147, 1665, 2567, 2677, 2974
BspANI	GGCC	2 cut(s)	214, 3090
BspCNI	CTCAG	4 cut(s)	1129, 1516, 1877, 2925
BspFNI	CGCG	2 cut(s)	2976, 2988
BspHI	TCATGA	1 cut(s)	1141
BspMAI	CTGCAG	2 cut(s)	1777, 2269
BspMI	ACCTGC	2 cut(s)	1720, 2422
BspQI	GCTCTTC	3 cut(s)	840, 2058, 2862
BspT104I	TTCGAA	1 cut(s)	3309
BspT107I	GGYRCC	2 cut(s)	856, 3211
BspTI	CTTAAG	1 cut(s)	2396
BsrBI	CCGCTC	1 cut(s)	1147
BsrDI	GCAATG	7 cut(s)	757, 1104, 2031, 2202, 2690, 2906, 3285
BsrGI	TGTACA	1 cut(s)	936
BsrI	ACTGG	6 cut(s)	990, 2104, 2328, 2826, 2947, 3048
BssECI	CCNNGG	5 cut(s)	432, 1107, 2594, 2980, 3336
BssSI	CACGAG	2 cut(s)	903, 2002
BssT1I	CCWWGG	4 cut(s)	1107, 2594, 2980, 3336
Bst2BI	CACGAG	2 cut(s)	903, 2002
Bst6I	CTCTTC	6 cut(s)	570, 840, 1610, 2058, 2208, 2862
BstAFI	CTTAAG	1 cut(s)	2396
BstAPI	GCANNNNNTGC	5 cut(s)	325, 948, 1099, 1103, 3287
BstAUI	TGTACA	1 cut(s)	936
BstBI	TTCGAA	1 cut(s)	3309
BstC8I	GCNNGC	5 cut(s)	321, 1095, 1104, 1149, 3318
BstDEI	CTNAG	9 cut(s)	11, 102, 292, 394, 1116, 1503, 1864, 2528, 2912
BstENI	CCTNNNNNAGG	1 cut(s)	2527
BstF5I	GGATG	9 cut(s)	820, 2112, 2429, 2445, 2604, 2728, 2812, 3235, 3281
BstFNI	CGCG	2 cut(s)	2976, 2988
BstHHI	GCGC	2 cut(s)	2076, 2990
BstMAI	GTCTC	7 cut(s)	552, 608, 1143, 1470, 1501, 1506, 1612
BstNSI	RCATGY	6 cut(s)	323, 500, 1908, 2358, 2747, 3320
BstPAI	GACNNNNGTC	1 cut(s)	2982
BstSFI	CTRYAG	6 cut(s)	1217, 1323, 1511, 1773, 2253, 2265
BstSLI	GKGCMC	1 cut(s)	1153
BstUI	CGCG	2 cut(s)	2976, 2988
BstV1I	GCAGC	9 cut(s)	433, 480, 1111, 2076, 2180, 2251, 2485, 2647, 3241
BstV2I	GAAGAC	1 cut(s)	3066
BstX2I	RGATCY	3 cut(s)	427, 2576, 3044
BstXI	CCANNNNNNTGG	2 cut(s)	1951, 3068
BstYI	RGATCY	3 cut(s)	427, 2576, 3044
Bsu36I	CCTNAGG	1 cut(s)	2528
BsuRI	GGCC	2 cut(s)	214, 3090
BtsCI	GGATG	9 cut(s)	820, 2112, 2429, 2445, 2604, 2728, 2812, 3235, 3281
BtsI	GCAGTG	5 cut(s)	330, 686, 878, 1782, 2064
BveI	ACCTGC	2 cut(s)	1720, 2422
Cac8I	GCNNGC	5 cut(s)	321, 1095, 1104, 1149, 3318
CaiI	CAGNNNCTG	2 cut(s)	1781, 2953
CciI	TCATGA	1 cut(s)	1141
CfoI	GCGC	2 cut(s)	2076, 2990
Cfr13I	GGNCC	9 cut(s)	59, 68, 436, 1059, 1080, 2326, 2533, 2956, 3089
CpoI	CGGWCCG	2 cut(s)	68, 1059
CseI	GACGC	1 cut(s)	1125
CspCI	CAANNNNNGTGG	6 cut(s)	1735, 1770, 2040, 2075, 3246, 3281
CspI	CGGWCCG	2 cut(s)	68, 1059
DdeI	CTNAG	9 cut(s)	11, 102, 292, 394, 1116, 1503, 1864, 2528, 2912
DraI	TTTAAA	2 cut(s)	822, 1474
DrdI	GACNNNNNNGTC	1 cut(s)	253
DseDI	GACNNNNNNGTC	1 cut(s)	253
Eam1104I	CTCTTC	6 cut(s)	570, 840, 1610, 2058, 2208, 2862
EarI	CTCTTC	6 cut(s)	570, 840, 1610, 2058, 2208, 2862
Ecl136II	GAGCTC	1 cut(s)	744
Eco130I	CCWWGG	4 cut(s)	1107, 2594, 2980, 3336
Eco24I	GRGCYC	2 cut(s)	636, 746
Eco32I	GATATC	1 cut(s)	2110
Eco47I	GGWCC	8 cut(s)	59, 68, 436, 1059, 1080, 2326, 2533, 2956
Eco53kI	GAGCTC	1 cut(s)	744
Eco57I	CTGAAG	3 cut(s)	1271, 2232, 2886
Eco81I	CCTNAGG	1 cut(s)	2528
Eco88I	CYCGRG	1 cut(s)	431
EcoICRI	GAGCTC	1 cut(s)	744
EcoNI	CCTNNNNNAGG	1 cut(s)	2527
EcoO109I	RGGNCCY	1 cut(s)	436
EcoRI	GAATTC	4 cut(s)	1003, 1672, 1878, 2344
EcoRV	GATATC	1 cut(s)	2110
EcoT14I	CCWWGG	4 cut(s)	1107, 2594, 2980, 3336
EcoT22I	ATGCAT	2 cut(s)	1855, 2392
EcoT38I	GRGCYC	2 cut(s)	636, 746
ErhI	CCWWGG	4 cut(s)	1107, 2594, 2980, 3336
Esp3I	CGTCTC	1 cut(s)	1143
FalI	AAGNNNNNCTT	6 cut(s)	236, 268, 1269, 1301, 2908, 2940
FaqI	GGGAC	2 cut(s)	1093, 2546
FauI	CCCGC	1 cut(s)	1140
FauNDI	CATATG	1 cut(s)	1660
FbaI	TGATCA	1 cut(s)	3295
FblI	GTMKAC	1 cut(s)	3223
FokI	GGATG	9 cut(s)	827, 2099, 2416, 2452, 2611, 2735, 2819, 3242, 3268
FriOI	GRGCYC	2 cut(s)	636, 746
FspBI	CTAG	6 cut(s)	747, 981, 1170, 1406, 2595, 2994
GlaI	GCGC	2 cut(s)	2075, 2989
GsaI	CCCAGC	4 cut(s)	565, 1671, 2683, 3197
GsuI	CTGGAG	1 cut(s)	1663
HaeIII	GGCC	2 cut(s)	214, 3090
HapII	CCGG	1 cut(s)	168
HgaI	GACGC	1 cut(s)	1125
HhaI	GCGC	2 cut(s)	2076, 2990
Hin6I	GCGC	2 cut(s)	2074, 2988
HinP1I	GCGC	2 cut(s)	2074, 2988
HincII	GTYRAC	2 cut(s)	1993, 3224
HindII	GTYRAC	2 cut(s)	1993, 3224
HindIII	AAGCTT	3 cut(s)	1629, 2399, 2547
HpaII	CCGG	1 cut(s)	168
HphI	GGTGA	3 cut(s)	1559, 1769, 3163
Hpy166II	GTNNAC	8 cut(s)	532, 1032, 1525, 1744, 1910, 1993, 2329, 3224
Hpy8I	GTNNAC	8 cut(s)	532, 1032, 1525, 1744, 1910, 1993, 2329, 3224
Hpy99I	CGWCG	5 cut(s)	31, 59, 632, 3225, 3228
HpyF3I	CTNAG	9 cut(s)	11, 102, 292, 394, 1116, 1503, 1864, 2528, 2912
HspAI	GCGC	2 cut(s)	2074, 2988
KpnI	GGTACC	2 cut(s)	860, 3215
Ksp22I	TGATCA	1 cut(s)	3295
LguI	GCTCTTC	3 cut(s)	840, 2058, 2862
LmnI	GCTCC	4 cut(s)	414, 1305, 2525, 2934
Lsp1109I	GCAGC	9 cut(s)	433, 480, 1111, 2076, 2180, 2251, 2485, 2647, 3241
LweI	GCATC	8 cut(s)	336, 428, 976, 1411, 1840, 2377, 2612, 3290
MaeI	CTAG	6 cut(s)	747, 981, 1170, 1406, 2595, 2994
MaeIII	GTNAC	8 cut(s)	141, 1592, 1757, 1777, 2017, 2683, 2832, 3143
MbiI	CCGCTC	1 cut(s)	1147
MflI	RGATCY	3 cut(s)	427, 2576, 3044
MhlI	GDGCHC	6 cut(s)	636, 746, 852, 972, 1117, 1153
MlyI	GAGTC	4 cut(s)	80, 1487, 1614, 2840
MmeI	TCCRAC	4 cut(s)	261, 1280, 1382, 2434
Mph1103I	ATGCAT	2 cut(s)	1855, 2392
MroXI	GAANNNNTTC	4 cut(s)	691, 1007, 1281, 2620
MseI	TTAA	9 cut(s)	201, 821, 1043, 1238, 1473, 1626, 1938, 2397, 3096
MslI	CAYNNNNRTG	3 cut(s)	501, 720, 1695
MspA1I	CMGCKG	5 cut(s)	1460, 1603, 1667, 2264, 2679
MspCI	CTTAAG	1 cut(s)	2396
MspI	CCGG	1 cut(s)	168
Mva1269I	GAATGC	1 cut(s)	727
MvnI	CGCG	2 cut(s)	2976, 2988
NdeI	CATATG	1 cut(s)	1660
NmuCI	GTSAC	5 cut(s)	1592, 1757, 1777, 2832, 3143
NsiI	ATGCAT	2 cut(s)	1855, 2392
NspI	RCATGY	6 cut(s)	323, 500, 1908, 2358, 2747, 3320
NspV	TTCGAA	1 cut(s)	3309
PaeI	GCATGC	2 cut(s)	323, 3320
PagI	TCATGA	1 cut(s)	1141
PciI	ACATGT	3 cut(s)	1904, 2354, 2743
PciSI	GCTCTTC	3 cut(s)	840, 2058, 2862
PcsI	WCGNNNNNNNCGW	1 cut(s)	910
PctI	GAATGC	1 cut(s)	727
PdmI	GAANNNNTTC	4 cut(s)	691, 1007, 1281, 2620
PflMI	CCANNNNNTGG	1 cut(s)	2953
PleI	GAGTC	4 cut(s)	80, 1486, 1614, 2839
PpsI	GAGTC	4 cut(s)	80, 1486, 1614, 2839
PpuMI	RGGWCCY	1 cut(s)	436
PscI	ACATGT	3 cut(s)	1904, 2354, 2743
PshAI	GACNNNNGTC	1 cut(s)	2982
PshBI	ATTAAT	1 cut(s)	1043
PsiI	TTATAA	1 cut(s)	782
Psp124BI	GAGCTC	1 cut(s)	746
Psp5II	RGGWCCY	1 cut(s)	436
PspFI	CCCAGC	4 cut(s)	561, 1667, 2679, 3193
PspPI	GGNCC	9 cut(s)	59, 68, 436, 1059, 1080, 2326, 2533, 2956, 3089
PspPPI	RGGWCCY	1 cut(s)	436
PstI	CTGCAG	2 cut(s)	1777, 2269
PstNI	CAGNNNCTG	2 cut(s)	1781, 2953
PsuI	RGATCY	3 cut(s)	427, 2576, 3044
PvuII	CAGCTG	3 cut(s)	1460, 1603, 2264
RseI	CAYNNNNRTG	3 cut(s)	501, 720, 1695
Rsr2I	CGGWCCG	2 cut(s)	68, 1059
RsrII	CGGWCCG	2 cut(s)	68, 1059
SacI	GAGCTC	1 cut(s)	746
SalI	GTCGAC	1 cut(s)	3222
SapI	GCTCTTC	3 cut(s)	840, 2058, 2862
SaqAI	TTAA	9 cut(s)	201, 821, 1043, 1238, 1473, 1626, 1938, 2397, 3096
Sau96I	GGNCC	9 cut(s)	59, 68, 436, 1059, 1080, 2326, 2533, 2956, 3089
ScaI	AGTACT	2 cut(s)	2191, 2461
SchI	GAGTC	4 cut(s)	80, 1487, 1614, 2840
SduI	GDGCHC	6 cut(s)	636, 746, 852, 972, 1117, 1153
SfaNI	GCATC	8 cut(s)	336, 428, 976, 1411, 1840, 2377, 2612, 3290
SfcI	CTRYAG	6 cut(s)	1217, 1323, 1511, 1773, 2253, 2265
SfuI	TTCGAA	1 cut(s)	3309
SgrDI	CGTCGACG	1 cut(s)	3222
SinI	GGWCC	8 cut(s)	59, 68, 436, 1059, 1080, 2326, 2533, 2956
SmiMI	CAYNNNNRTG	3 cut(s)	501, 720, 1695
SmlI	CTYRAG	5 cut(s)	337, 1159, 1582, 2117, 2396
SmoI	CTYRAG	5 cut(s)	337, 1159, 1582, 2117, 2396
SpeI	ACTAGT	1 cut(s)	2993
SphI	GCATGC	2 cut(s)	323, 3320
SsiI	CCGC	8 cut(s)	215, 465, 1057, 1147, 1665, 2567, 2677, 2974
SspI	AATATT	1 cut(s)	1336
SspMI	CTAG	6 cut(s)	747, 981, 1170, 1406, 2595, 2994
SstI	GAGCTC	1 cut(s)	746
StyI	CCWWGG	4 cut(s)	1107, 2594, 2980, 3336
TaqI	TCGA	5 cut(s)	29, 84, 2370, 3223, 3309
TaqII	GACCGA	1 cut(s)	56
TatI	WGTACW	6 cut(s)	183, 731, 936, 1605, 2189, 2459
TauI	GCSGC	2 cut(s)	217, 1667
Tru1I	TTAA	9 cut(s)	201, 821, 1043, 1238, 1473, 1626, 1938, 2397, 3096
Tru9I	TTAA	9 cut(s)	201, 821, 1043, 1238, 1473, 1626, 1938, 2397, 3096
TseFI	GTSAC	5 cut(s)	1592, 1757, 1777, 2832, 3143
TseI	GCWGC	9 cut(s)	446, 493, 1124, 2089, 2168, 2264, 2498, 2660, 3254
Tsp45I	GTSAC	5 cut(s)	1592, 1757, 1777, 2832, 3143
TspGWI	ACGGA	6 cut(s)	60, 129, 297, 440, 902, 2714
Van91I	CCANNNNNTGG	1 cut(s)	2953
Vha464I	CTTAAG	1 cut(s)	2396
VpaK11BI	GGWCC	8 cut(s)	59, 68, 436, 1059, 1080, 2326, 2533, 2956
VspI	ATTAAT	1 cut(s)	1043
XagI	CCTNNNNNAGG	1 cut(s)	2527
XapI	RAATTY	7 cut(s)	1003, 1672, 1748, 1878, 2344, 2616, 2702
XceI	RCATGY	6 cut(s)	323, 500, 1908, 2358, 2747, 3320
XmaJI	CCTAGG	1 cut(s)	2594
XmiI	GTMKAC	1 cut(s)	3223
XmnI	GAANNNNTTC	4 cut(s)	691, 1007, 1281, 2620
XspI	CTAG	6 cut(s)	747, 981, 1170, 1406, 2595, 2994
ZrmI	AGTACT	2 cut(s)	2191, 2461
Zsp2I	ATGCAT	2 cut(s)	1855, 2392

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.