Rorug07G0285700

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0012629
Physical Location & Seq
Forward (+)
27765924 .. 27770241
4318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0285700.1

Sequence Viewer

Length: 2808 bp
ATGGATTTGATCTGGGTTTGTCCCTTGGTGTTTCTTTATCTTGGGGTGTTCTCATTTGGGTCTAGTAATAATCTTTCTTCAAGACCAGCTGCTGTCAACATTGGAGCTCTTTTCACGTATGACTCTACGATTGGTAAGGTTGCCAAGATTGCTATTGAGGAAGCGGTAAAAGATGTCAACTCCAATTTCAGCATTCTCCATGGGACCAAACTTGTTGCGAAATTTCAGAATACCAATTGCAGTGGGTTTCTTGGAATGGTTGAAGCTTTGCAATTCATGGAGACTGATATCGTTGCCATTATAGGCCCACAATCTTCTGTGGTTGCCCATATTGTATCCCACATTGCAAATGAACTCCAAGTACCACTATTGTCATTTGCAGCCACAGACCCAACTCTCTCAGCCCTTCAGTTCCCATATTTTATTAGGACAACACAAAGTGATTTGTATCAAATGACTGCAGTTGCTCAAATTGTTGATCATTATGGTTGGAGAGAGGTTATTGCCATTTTTGTAGATGATGATTATGGGCGTAATGGCATATCAGCTTTAGATGATAAACTTGCTGAAAGGCGCTGTAAAATTTCCTACAAGTTGGCAATTCCCCCAGGACCGGCAGCTAATCGGAGTGCCATCATGGATCTGCTTATTAAGGTTGCCTTATTGGAATCTCGCGTTATAGTTCTTCATGTGAATCCTGATTCAGGTTTCATGGTTCTTGCTGTGGCACAGTATCTTAAAATGATGGGTGATGGTTTTGTATGGATAGCTACAGATTGGCTTTCATCTGTTTTAGATTCTGCTTTTCCTCTTCCTTCTGAAATCATGGATACACTGCAAGGAGTTCTTGTTTTGCGGCTACATACACCAGATTCAGATAGAAAGAGGGCCTTCTTTTCTAAGTGGAACAAGCTAACCAGTGGTTCTTTGGGACTGCATACTTATGGGCTTCATGCTTATGATTCTGTTTGGCTGTTAGCTCATGCTATTGATGCGTTTTTCAACCAGGGTGGGGTTATTTCGTTCTCTAATGATTCTAGGATAAAAGCTGTAGAACAAGGTGATAGTCTTCACCTTGACGCAATGAGCATTTTTGATGATGGACCACTCCTGTTGAAGAACATATTGCAAAGTAACCTTGTTGGTTTGACAGGTCCCATTAAGTTTGATTCAGAAAGGGCTCTTGCTCTTCCAGCATATGATATTATTAATGTGGTTGGAACTGGGTTTCGACGAATTGGTTACTGGTCCAACTATTCTGGGTTATCAACTGTACCTCCTGAGACTCTCTATTCAAAGCCACCTAATCGTTCAAGTGCAAATCAACAGTTATATAGTGTTATCTGGCCAGGGGAGACATTATTGAAGCCCCGTGGCTGGGTTTTCCCAAACAATGGGATACAATTGAGAATTGGTGTACCTATTCGGGTCAGTTACCGGGAGTTTGTAATGCCAGTTCAGGGAACTGATACTTTCAAGGGTTTCTGCATAGATGTATTCAATGCCGCTGTAAACTTGTTGCCATATGCTGTTCCATACAAGTTTATCCCTTTTGGAGATGGTCATAAAAACCCAAACTACACAGAGCTTGTGACTTCTATCACAACGGGGGTCTTTGATGCTGCAATTGGCGACATTGCCATTGTTACAGATCGAACGAAGATGGTGGATTTTACACAGCCATATGCTGCATCTGGACTTGTTGTAGTGGCTCCCTTTAAAAAATTGAACTCTGGTGCTTGGACCTTCCTTCGACCATTTACTGCACACATGTGGATAGTCACTGCTGCTTCCTTTTTTGTTATTGGTATAGTTGTATGGATTTTGGAGCATAGGATAAATGATGAATTCAGGGGTCCACCGAAGAAGCAACTTATAACCATCTTGTGGTTTAGCCTCTCAACTCTATTCTTTGCCCATAGAGAGAACACTGTGAGCACCCTTGGTCGAGTAGTGCTACTTATATGGCTGTTTGTGGTTTTGATAATCAACTCTAGTTACACTGCTAGTCTGACATCAATCCTCACAGTGCAGCAGCTATCTTCTCCTATCAAAGGAATGGAAAGCTTGAAAAACAGTGGCGAGCCAATCGGGTATCAAGTGGGTTCTTTTGCTGAGCATTATTTGAGTGAGGAGCTCGGCATATCCAAGTCCAGGCTCATTCCCCTTGCCTCTCCTCTAGCCTATGCTCAGGCACTTCAGCTTGGTCCTAAAAAGGGAGGTGTTGCTGCCGTGGTTGATGAACGCCCGTATGTTGAACTATTCCTCTCAGCACAGTGCAAATTTCGGATTCTAGGTCAAGAGTTCACCAAAGGCGGCTGGGGTTTTGCATTTCCACGAGACTCTCCTTTAGCTGTTGACATATCAACTGCGATTCTACAATTATCGGAGAATGGTGATCTGCAACGGATCCATGATAAGTGGTTGGTGCAAACCACTTGCAGTTTTGAGAGCACTGAAATTGAATCAGACCAGCTTCAACTCAGAAGCTTTTGGGGCCTCTTTGTCATATGTGGAATTGCTTGCTTCATTGCTCTCCTTGTATATTTTTTGCAAATTGCGAACAAGTTACGCCGTGCTGACCCCCCGCAGAGTGTTTCAACTAGTCCTGGTGTCTCACAATCTGGGCGAGTCCGGCGATTTTTATCATTAATTGACAAGAAGGCTGACCAGTCCGGTAGTGGAAGTAAAAAAAGGAGAGTTATTAGATCACTATCCGACAATGATAAGGATGGCAAGTTTGGTTGGACTCCCAAGAAGAAACAAGCAGAAATGACTGATATGGGTGACATCAGGTCTAGCAGTTAA

Protein Analysis

935

Amino Acids

103.21

Weight (kDa)

8.19

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 43 - 333 1.1e-11 Periplasmic binding protein
ANF_receptor PF01094 49 - 407 2.6e-93 Receptor family ligand binding region
SBP_bac_3 PF00497 477 - 809 2.4e-11 Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd PF10613 483 - 571 1.9e-09 Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan PF00060 589 - 842 3.1e-39 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1877
AccB7I CCANNNNNTGG 2 cut(s) 1394, 1887
AccII CGCG 1 cut(s) 675
AciI CCGC 5 cut(s) 164, 856, 1506, 2316, 2588
AclWI GGATC 3 cut(s) 648, 2404, 2417
AcoI YGGCCR 1 cut(s) 1346
AcsI RAATTY 4 cut(s) 221, 582, 1847, 2282
AcuI CTGAAG 2 cut(s) 392, 2183
AdeI CACNNNGTG 1 cut(s) 440
AfaI GTAC 3 cut(s) 363, 1275, 1419
AflIII ACRYGT 1 cut(s) 1770
AhdI GACNNNNNGTC 1 cut(s) 2794
AhlI ACTAGT 1 cut(s) 2603
AjnI CCWGG 5 cut(s) 607, 1005, 1348, 2153, 2608
AleI CACNNNNGTG 1 cut(s) 1771
Alw21I GWGCWC 4 cut(s) 109, 1940, 2139, 2456
Alw26I GTCTC 5 cut(s) 275, 1277, 1349, 2334, 2620
AlwI GGATC 3 cut(s) 648, 2404, 2417
AlwNI CAGNNNCTG 1 cut(s) 92
AoxI GGCC 4 cut(s) 304, 888, 1346, 2497
ApeKI GCWGC 9 cut(s) 89, 380, 617, 1622, 1688, 1787, 2032, 2035, 2228
ApoI RAATTY 4 cut(s) 221, 582, 1847, 2282
AseI ATTAAT 2 cut(s) 1209, 2651
AspLEI GCGC 1 cut(s) 576
AsuC2I CCSGG 1 cut(s) 1439
AsuHPI GGTGA 6 cut(s) 761, 1064, 1073, 2299, 2408, 2798
AvaII GGWCC 8 cut(s) 204, 611, 1103, 1154, 1248, 1743, 1856, 2207
BalI TGGCCA 1 cut(s) 1348
BamHI GGATCC 1 cut(s) 2409
BanII GRGCYC 3 cut(s) 109, 1183, 2139
BauI CACGAG 1 cut(s) 2337
BbsI GAAGAC 1 cut(s) 1061
Bbv12I GWGCWC 4 cut(s) 109, 1940, 2139, 2456
BbvI GCAGC 9 cut(s) 76, 392, 629, 1609, 1675, 1774, 2044, 2047, 2215
BccI CCATC 8 cut(s) 641, 739, 746, 1094, 1553, 1657, 1889, 2726
BceAI ACGGC 2 cut(s) 2216, 2559
BciT130I CCWGG 5 cut(s) 609, 1007, 1350, 2155, 2610
BciVI GTATCC 3 cut(s) 346, 823, 1392
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 1 cut(s) 1439
BcoDI GTCTC 5 cut(s) 275, 1277, 1349, 2334, 2620
BcuI ACTAGT 1 cut(s) 2603
BfaI CTAG 8 cut(s) 63, 1038, 1995, 2007, 2180, 2294, 2604, 2799
BfmI CTRYAG 3 cut(s) 459, 771, 1050
BfoI RGCGCY 1 cut(s) 577
BfuI GTATCC 3 cut(s) 346, 823, 1392
BlpI GCTNAGC 1 cut(s) 2115
Bme1390I CCNGG 6 cut(s) 609, 1007, 1350, 1439, 2155, 2610
Bme18I GGWCC 8 cut(s) 204, 611, 1103, 1154, 1248, 1743, 1856, 2207
BmeRI GACNNNNNGTC 1 cut(s) 2794
BmiI GGNNCC 6 cut(s) 205, 1156, 1713, 1857, 2411, 2498
BmrFI CCNGG 6 cut(s) 609, 1007, 1350, 1439, 2155, 2610
BmrI ACTGGG 1 cut(s) 1233
BmsI GCATC 3 cut(s) 982, 1609, 1700
BmuI ACTGGG 1 cut(s) 1233
BpiI GAAGAC 1 cut(s) 1061
Bpu10I CCTNAGC 1 cut(s) 2190
Bpu1102I GCTNAGC 1 cut(s) 2115
BpuMI CCSGG 1 cut(s) 1439
BsaAI YACGTR 1 cut(s) 117
BsaBI GATNNNNATC 3 cut(s) 447, 2644, 2713
BsaJI CCNNGG 8 cut(s) 24, 199, 607, 1006, 1349, 1372, 1942, 2232
BsaWI WCCGGW 1 cut(s) 2675
BsaXI ACNNNNNCTCC 4 cut(s) 834, 864, 1261, 1291
Bse118I RCCGGY 1 cut(s) 613
Bse1I ACTGG 5 cut(s) 918, 1228, 1250, 1454, 2671
Bse3DI GCAATG 4 cut(s) 342, 1089, 1635, 2529
Bse8I GATNNNNATC 3 cut(s) 447, 2644, 2713
BseBI CCWGG 5 cut(s) 609, 1007, 1350, 2155, 2610
BseDI CCNNGG 8 cut(s) 24, 199, 607, 1006, 1349, 1372, 1942, 2232
BseGI GGATG 1 cut(s) 2737
BseJI GATNNNNATC 3 cut(s) 447, 2644, 2713
BseMI GCAATG 4 cut(s) 342, 1089, 1635, 2529
BseMII CTCAG 6 cut(s) 414, 1272, 2106, 2204, 2283, 2497
BseNI ACTGG 5 cut(s) 918, 1228, 1250, 1454, 2671
BseRI GAGGAG 2 cut(s) 2147, 2166
BseXI GCAGC 9 cut(s) 76, 392, 629, 1609, 1675, 1774, 2044, 2047, 2215
BseYI CCCAGC 2 cut(s) 1377, 2319
BsgI GTGCAG 2 cut(s) 1749, 2051
Bsh1236I CGCG 1 cut(s) 675
BshFI GGCC 4 cut(s) 306, 890, 1348, 2499
BsiHKAI GWGCWC 4 cut(s) 109, 1940, 2139, 2456
BsiSI CCGG 4 cut(s) 614, 1438, 2635, 2676
BslFI GGGAC 4 cut(s) 6, 217, 945, 1140
BsmAI GTCTC 5 cut(s) 275, 1277, 1349, 2334, 2620
BsmFI GGGAC 4 cut(s) 6, 217, 945, 1140
BsmI GAATGC 1 cut(s) 192
BsnI GGCC 4 cut(s) 306, 890, 1348, 2499
Bsp1286I GDGCHC 5 cut(s) 109, 1183, 1940, 2139, 2456
Bsp143I GATC 7 cut(s) 9, 478, 640, 1651, 2398, 2409, 2708
Bsp1720I GCTNAGC 1 cut(s) 2115
Bsp19I CCATGG 1 cut(s) 199
BspACI CCGC 5 cut(s) 164, 856, 1506, 2316, 2588
BspANI GGCC 4 cut(s) 306, 890, 1348, 2499
BspCNI CTCAG 6 cut(s) 413, 1273, 2107, 2203, 2282, 2496
BspFNI CGCG 1 cut(s) 675
BspLI GGNNCC 6 cut(s) 205, 1156, 1713, 1857, 2411, 2498
BspMAI CTGCAG 1 cut(s) 463
BspPI GGATC 3 cut(s) 648, 2404, 2417
BspQI GCTCTTC 1 cut(s) 1194
BsrDI GCAATG 4 cut(s) 342, 1089, 1635, 2529
BsrFI RCCGGY 1 cut(s) 613
BsrI ACTGG 5 cut(s) 918, 1228, 1250, 1454, 2671
BssAI RCCGGY 1 cut(s) 613
BssECI CCNNGG 8 cut(s) 24, 199, 607, 1006, 1349, 1372, 1942, 2232
BssMI GATC 7 cut(s) 9, 478, 640, 1651, 2398, 2409, 2708
BssSI CACGAG 1 cut(s) 2337
BssT1I CCWWGG 3 cut(s) 24, 199, 1942
Bst2BI CACGAG 1 cut(s) 2337
Bst2UI CCWGG 5 cut(s) 609, 1007, 1350, 2155, 2610
Bst4CI ACNGT 7 cut(s) 732, 1273, 1329, 1933, 2029, 2078, 2277
Bst6I CTCTTC 2 cut(s) 816, 1194
BstBAI YACGTR 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 2084, 2524
BstDEI CTNAG 7 cut(s) 400, 900, 1281, 2115, 2190, 2269, 2483
BstDSI CCRYGG 3 cut(s) 199, 1372, 2232
BstENI CCTNNNNNAGG 2 cut(s) 702, 2052
BstF5I GGATG 1 cut(s) 2737
BstFNI CGCG 1 cut(s) 675
BstH2I RGCGCY 1 cut(s) 577
BstHHI GCGC 1 cut(s) 576
BstKTI GATC 7 cut(s) 12, 481, 643, 1654, 2401, 2412, 2711
BstMAI GTCTC 5 cut(s) 275, 1277, 1349, 2334, 2620
BstMBI GATC 7 cut(s) 9, 478, 640, 1651, 2398, 2409, 2708
BstMWI GCNNNNNNNGC 5 cut(s) 149, 992, 1193, 2496, 2635
BstNI CCWGG 5 cut(s) 609, 1007, 1350, 2155, 2610
BstNSI RCATGY 1 cut(s) 1774
BstSCI CCNGG 6 cut(s) 607, 1005, 1348, 1437, 2153, 2608
BstSFI CTRYAG 3 cut(s) 459, 771, 1050
BstUI CGCG 1 cut(s) 675
BstV1I GCAGC 9 cut(s) 76, 392, 629, 1609, 1675, 1774, 2044, 2047, 2215
BstV2I GAAGAC 1 cut(s) 1061
BstX2I RGATCY 2 cut(s) 640, 2409
BstYI RGATCY 2 cut(s) 640, 2409
BsuI GTATCC 3 cut(s) 346, 823, 1392
BsuRI GGCC 4 cut(s) 306, 890, 1348, 2499
BtgI CCRYGG 3 cut(s) 199, 1372, 2232
BtsCI GGATG 1 cut(s) 2737
BtsI GCAGTG 4 cut(s) 247, 833, 1782, 2001
Cac8I GCNNGC 2 cut(s) 2084, 2524
CaiI CAGNNNCTG 1 cut(s) 92
CfoI GCGC 1 cut(s) 576
Cfr10I RCCGGY 1 cut(s) 613
CseI GACGC 1 cut(s) 1088
Csp6I GTAC 3 cut(s) 362, 1274, 1418
CspCI CAANNNNNGTGG 6 cut(s) 223, 258, 991, 1026, 1095, 1130
CviQI GTAC 3 cut(s) 362, 1274, 1418
DdeI CTNAG 7 cut(s) 400, 900, 1281, 2115, 2190, 2269, 2483
DpnI GATC 7 cut(s) 11, 480, 642, 1653, 2400, 2411, 2710
DpnII GATC 7 cut(s) 9, 478, 640, 1651, 2398, 2409, 2708
DraI TTTAAA 1 cut(s) 1720
DraIII CACNNNGTG 1 cut(s) 440
DriI GACNNNNNGTC 1 cut(s) 2794
EaeI YGGCCR 1 cut(s) 1346
Eam1104I CTCTTC 2 cut(s) 816, 1194
Eam1105I GACNNNNNGTC 1 cut(s) 2794
EarI CTCTTC 2 cut(s) 816, 1194
Ecl136II GAGCTC 2 cut(s) 107, 2137
Eco130I CCWWGG 3 cut(s) 24, 199, 1942
Eco24I GRGCYC 3 cut(s) 109, 1183, 2139
Eco32I GATATC 1 cut(s) 289
Eco47I GGWCC 8 cut(s) 204, 611, 1103, 1154, 1248, 1743, 1856, 2207
Eco53kI GAGCTC 2 cut(s) 107, 2137
Eco57I CTGAAG 2 cut(s) 392, 2183
EcoICRI GAGCTC 2 cut(s) 107, 2137
EcoNI CCTNNNNNAGG 2 cut(s) 702, 2052
EcoO109I RGGNCCY 3 cut(s) 888, 1154, 2497
EcoRI GAATTC 1 cut(s) 1847
EcoRII CCWGG 5 cut(s) 607, 1005, 1348, 2153, 2608
EcoRV GATATC 1 cut(s) 289
EcoT14I CCWWGG 3 cut(s) 24, 199, 1942
EcoT38I GRGCYC 3 cut(s) 109, 1183, 2139
ErhI CCWWGG 3 cut(s) 24, 199, 1942
FalI AAGNNNNNCTT 6 cut(s) 644, 676, 831, 863, 875, 907
FaqI GGGAC 4 cut(s) 6, 217, 945, 1140
FauI CCCGC 1 cut(s) 2595
FauNDI CATATG 4 cut(s) 1198, 1525, 1684, 2510
FbaI TGATCA 1 cut(s) 478
FokI GGATG 1 cut(s) 2744
FriOI GRGCYC 3 cut(s) 109, 1183, 2139
FspBI CTAG 8 cut(s) 63, 1038, 1995, 2007, 2180, 2294, 2604, 2799
GlaI GCGC 1 cut(s) 575
GsaI CCCAGC 2 cut(s) 1381, 2323
HaeII RGCGCY 1 cut(s) 577
HaeIII GGCC 4 cut(s) 306, 890, 1348, 2499
HapII CCGG 4 cut(s) 614, 1438, 2635, 2676
HgaI GACGC 1 cut(s) 1088
HhaI GCGC 1 cut(s) 576
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HincII GTYRAC 3 cut(s) 97, 178, 2359
HindII GTYRAC 3 cut(s) 97, 178, 2359
HindIII AAGCTT 3 cut(s) 264, 2065, 2488
HpaII CCGG 4 cut(s) 614, 1438, 2635, 2676
HphI GGTGA 6 cut(s) 761, 1064, 1073, 2299, 2408, 2798
Hpy166II GTNNAC 7 cut(s) 97, 178, 1418, 1513, 1859, 2307, 2359
Hpy188III TCNNGA 5 cut(s) 81, 698, 1280, 1695, 2300
Hpy8I GTNNAC 7 cut(s) 97, 178, 1418, 1513, 1859, 2307, 2359
Hpy99I CGWCG 1 cut(s) 1236
HpyAV CCTTC 6 cut(s) 416, 825, 901, 1756, 1760, 2656
HpyCH4III ACNGT 7 cut(s) 732, 1273, 1329, 1933, 2029, 2078, 2277
HpyCH4IV ACGT 1 cut(s) 116
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 992, 1193, 2496, 2635
HpyF3I CTNAG 7 cut(s) 400, 900, 1281, 2115, 2190, 2269, 2483
HpySE526I ACGT 1 cut(s) 116
HspAI GCGC 1 cut(s) 574
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 7 cut(s) 9, 478, 640, 1651, 2398, 2409, 2708
LguI GCTCTTC 1 cut(s) 1194
LmnI GCTCC 4 cut(s) 104, 1717, 1828, 2134
Lsp1109I GCAGC 9 cut(s) 76, 392, 629, 1609, 1675, 1774, 2044, 2047, 2215
LweI GCATC 3 cut(s) 982, 1609, 1700
MaeI CTAG 8 cut(s) 63, 1038, 1995, 2007, 2180, 2294, 2604, 2799
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 9 cut(s) 1133, 1241, 1433, 1591, 1645, 1780, 1997, 2568, 2786
MalI GATC 7 cut(s) 11, 480, 642, 1653, 2400, 2411, 2710
MboI GATC 7 cut(s) 9, 478, 640, 1651, 2398, 2409, 2708
MfeI CAATTG 3 cut(s) 235, 1403, 1626
MflI RGATCY 2 cut(s) 640, 2409
MhlI GDGCHC 5 cut(s) 109, 1183, 1940, 2139, 2456
MlsI TGGCCA 1 cut(s) 1348
MluNI TGGCCA 1 cut(s) 1348
MlyI GAGTC 5 cut(s) 116, 1279, 2336, 2640, 2743
MmeI TCCRAC 5 cut(s) 470, 1198, 1275, 2726, 2742
Mox20I TGGCCA 1 cut(s) 1348
MscI TGGCCA 1 cut(s) 1348
MseI TTAA 7 cut(s) 651, 738, 1161, 1209, 1719, 2651, 2806
MslI CAYNNNNRTG 3 cut(s) 942, 957, 1771
Msp20I TGGCCA 1 cut(s) 1348
MspA1I CMGCKG 2 cut(s) 89, 1508
MspI CCGG 4 cut(s) 614, 1438, 2635, 2676
MspR9I CCNGG 6 cut(s) 609, 1007, 1350, 1439, 2155, 2610
MunI CAATTG 3 cut(s) 235, 1403, 1626
Mva1269I GAATGC 1 cut(s) 192
MvaI CCWGG 5 cut(s) 609, 1007, 1350, 2155, 2610
MvnI CGCG 1 cut(s) 675
MwoI GCNNNNNNNGC 5 cut(s) 149, 992, 1193, 2496, 2635
NciI CCSGG 1 cut(s) 1439
NcoI CCATGG 1 cut(s) 199
NdeI CATATG 4 cut(s) 1198, 1525, 1684, 2510
NdeII GATC 7 cut(s) 9, 478, 640, 1651, 2398, 2409, 2708
NlaIV GGNNCC 6 cut(s) 205, 1156, 1713, 1857, 2411, 2498
NmeAIII GCCGAG 1 cut(s) 2118
NmuCI GTSAC 3 cut(s) 1591, 1780, 2786
NspI RCATGY 1 cut(s) 1774
OliI CACNNNNGTG 1 cut(s) 1771
PciI ACATGT 1 cut(s) 1770
PciSI GCTCTTC 1 cut(s) 1194
PctI GAATGC 1 cut(s) 192
PflMI CCANNNNNTGG 2 cut(s) 1394, 1887
PleI GAGTC 5 cut(s) 116, 1279, 2336, 2639, 2743
PpsI GAGTC 5 cut(s) 116, 1279, 2336, 2639, 2743
Ppu21I YACGTR 1 cut(s) 117
PpuMI RGGWCCY 1 cut(s) 1154
PscI ACATGT 1 cut(s) 1770
PshBI ATTAAT 2 cut(s) 1209, 2651
PsiI TTATAA 1 cut(s) 1877
Psp124BI GAGCTC 2 cut(s) 109, 2139
Psp5II RGGWCCY 1 cut(s) 1154
Psp6I CCWGG 5 cut(s) 607, 1005, 1348, 2153, 2608
PspFI CCCAGC 2 cut(s) 1377, 2319
PspGI CCWGG 5 cut(s) 607, 1005, 1348, 2153, 2608
PspN4I GGNNCC 6 cut(s) 205, 1156, 1713, 1857, 2411, 2498
PspPPI RGGWCCY 1 cut(s) 1154
PsrI GAACNNNNNNTAC 4 cut(s) 345, 377, 1440, 1472
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 2 cut(s) 640, 2409
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 3 cut(s) 363, 1275, 1419
RsaNI GTAC 3 cut(s) 362, 1274, 1418
RseI CAYNNNNRTG 3 cut(s) 942, 957, 1771
SacI GAGCTC 2 cut(s) 109, 2139
SapI GCTCTTC 1 cut(s) 1194
SaqAI TTAA 7 cut(s) 651, 738, 1161, 1209, 1719, 2651, 2806
Sau3AI GATC 7 cut(s) 9, 478, 640, 1651, 2398, 2409, 2708
SchI GAGTC 5 cut(s) 116, 1279, 2336, 2640, 2743
ScrFI CCNGG 6 cut(s) 609, 1007, 1350, 1439, 2155, 2610
SduI GDGCHC 5 cut(s) 109, 1183, 1940, 2139, 2456
SfaNI GCATC 3 cut(s) 982, 1609, 1700
SfcI CTRYAG 3 cut(s) 459, 771, 1050
SinI GGWCC 8 cut(s) 204, 611, 1103, 1154, 1248, 1743, 1856, 2207
SmiMI CAYNNNNRTG 3 cut(s) 942, 957, 1771
SpeI ACTAGT 1 cut(s) 2603
SsiI CCGC 5 cut(s) 164, 856, 1506, 2316, 2588
SspMI CTAG 8 cut(s) 63, 1038, 1995, 2007, 2180, 2294, 2604, 2799
SstI GAGCTC 2 cut(s) 109, 2139
StyD4I CCNGG 6 cut(s) 607, 1005, 1348, 1437, 2153, 2608
StyI CCWWGG 3 cut(s) 24, 199, 1942
TaaI ACNGT 7 cut(s) 732, 1273, 1329, 1933, 2029, 2078, 2277
TaiI ACGT 1 cut(s) 119
TaqI TCGA 4 cut(s) 1231, 1654, 1753, 1948
TauI GCSGC 3 cut(s) 859, 1508, 2319
Tru1I TTAA 7 cut(s) 651, 738, 1161, 1209, 1719, 2651, 2806
Tru9I TTAA 7 cut(s) 651, 738, 1161, 1209, 1719, 2651, 2806
TseFI GTSAC 3 cut(s) 1591, 1780, 2786
TseI GCWGC 9 cut(s) 89, 380, 617, 1622, 1688, 1787, 2032, 2035, 2228
Tsp45I GTSAC 3 cut(s) 1591, 1780, 2786
TspDTI ATGAA 9 cut(s) 265, 366, 677, 700, 774, 941, 1860, 2256, 2518
TspGWI ACGGA 1 cut(s) 2422
Van91I CCANNNNNTGG 2 cut(s) 1394, 1887
VpaK11BI GGWCC 8 cut(s) 204, 611, 1103, 1154, 1248, 1743, 1856, 2207
VspI ATTAAT 2 cut(s) 1209, 2651
XagI CCTNNNNNAGG 2 cut(s) 702, 2052
XapI RAATTY 4 cut(s) 221, 582, 1847, 2282
XceI RCATGY 1 cut(s) 1774
XcmI CCANNNNNNNNNTGG 2 cut(s) 925, 2678
XspI CTAG 8 cut(s) 63, 1038, 1995, 2007, 2180, 2294, 2604, 2799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.