Rw7G036610

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012629
Physical Location & Seq
Forward (+)
55356282 .. 55371235
14954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G036610.1

Sequence Viewer

Length: 10938 bp
ATGACTACTGTAAGATCAAGCTTGCCGTCCAGGATTCGGCAGCTTCTGTCCGCCGACGGTGCTCTCGGGCCGTCTATCAAGCTCGACTCCGAGCCGTCTCCTAAGATCAAAGCCTTTATCAATAAGGTGATACAGTGTCCATTACAAGATATAGCTATTCCTCTTTCCGGTTTCCGCTGGGAGTACAGTAAGGGTAATTTCAACCATTGGAGGCCGCTTTTTCTGCATTTCGATACATATTTCAAGACATACTTGTCTGGTAGAAATGACCTACTCTTGTCCGATAAAATCTTAGAAGATGATACTCCATTCCCAAAACATGCAGTACTACAAATTTTGAGAGTGATGCAAACAATTTTAGAGAATTGCCATAACAAGAGTTCTTTTGATGGCTTAGAGCATTTCAAGCTGCTACTTGCATCAACAGATCCAGAAGTTCTTATAGCAACACTCGAAACACTTTCTGCGCTTGTGAAAATATATCCCTCTAAGATGCATGTCAGTGGAAAGATGATCGGCTGTGGTTCTGTAAACAGCTATCTCCTGTCGCTTGCACAGGGCTGGGGAAGCAAGGAAGAAGGGTTGGGTCTATTTTCATGTGTCATGGCAAACGAGACTGCCCAAGATGACGGGCTGAATTTGTTCCCATCTGATGTAGAAAATGATTCTGACAAATCTCAGCATCGAATAGGTTCTACCCTTTATTTTGAATTGCATGGGAATGCCCAAAGTACAGAGGAGAGCAGTAGCAATGTAAATAGTACTTCCAGTTTGGGGGTTATAAACATGCCAGATCTGCATTTGCAGAAGGAGGATGATTTAAAGCTGATGAAACAGTGCATAGAGGACTACAGGGTACCTCCTGAGATTCGGTTCTCACTCCTGACAAGAATCAGATATGCTCGTGCCTTCCGTTCACCAAGAATCTGCAGGTTGTATAGCAGGATTTGCCTTCTTGCATTTATTGTGCTTGTTCAATCTAGTGATGCACATGAAGAACTTGTATCATTCTTTGCTAATGAGCCAGAATATACTAATGAACTGATTAGAATTGTGCGGTCGGAAGAATCAGTTTCTGGAACCATCAGAACACATGCAATGCTTGCCTTGGGAGCTCAATTAGCTGCTTATTCTGCATCTCATGAGCGGGCACGAATTTTGAGTGGATCTAGTATCAGTTTTGCAGGAGGGAATCGTATGATACTTCTGAATGTACTCCAGAGAGCAGTTTTATCACTTAAAACCTCCAATGATCCAACTTCTCTTGCTTTTGTTGAAGCTCTGCTTCAGTTCTATTTGCTCCATGTGGTTTCATCGTCCACTACAGGGAGTAATATTAGAGGGTCAGGCATGGTTCCAACATTCTTGCCTCTTTTGGAGGATTCTGATCCCTTGCACTTGCATCTAGTCTGTTTTGCTGTGAAAACTCTGCAAAAGCTCATGGATTACAGTAGTTCAGCTGTGTCTCTCTTTAAAGAGTTAGGGGGAGTTGAACTGTTGGCTCAGAGACTACAGATAGAGGTACATAGGGTGATAGGCTTGGTCGGCGATAATGATAATTCAATGGTCATTGGTGAAAGCTCCAGATATAATGAAGACCAGTTGTACTCCCAGAAGAGGCTCATTAAAGCTTCGTTGAAGGCACTTGGATCTGCTTCATATGCTGCTGGCAATTCTACCAGATCTCAGCATTCTCATGACAGTTCTTTACCTGAAACTCTTTCTCTGATCTTTGGGAACGTTGATAAGTTTGGAGGTGACATTTATCACTCTGCTGTGACAGTTCTGAGTGAAACGATACACAAAGACCCAACTTGTTATTCTGCTTTGCATGATATGGGTCTTCCAGATGCTTTCATATCTTCAGTTGTAGCTGGGGTTCTTCCATCTGCAAAGGCACTTCAGTGTGTGCCTAATGGTCTCGGTGCTATATGTCTTAACGCCAAAGGCTTGGAGGCTGTGAAGGAAAAGTTGGCCTTACGATTTCTTGTTGACATCTTCACCAGCAAGAAGTATGTAGTAGCAATGAATGGAGCTATTGTCCATTTGACAAATGCTGTGGAAGAGCTTTTGCGCCATGTATCTTCGCTAAGAAGTATTGGTGTTGATATTATGATTGAAATTGTTGAAAAAATTGCTTCCTTCACTGACAACAACAATGCAGGGCTAGCAGGAAAGGCTAGTGGGAGTGGAGAACAGCCTTTGGTAGGTGCTGTAGATTCGGCTGCAGATGGTATCAGTAATGATCAGTTCATTCAATTATCCATTTTCCATTTGATGGTACTGGTCCACAGAACAATGGAGAATGCTGAAATATGTCGGTTATTTGTTGAAAAGTCTGGAATTGATGCTTTACTGAAGCTTTTGTTACAGCCAACTGTTGTACAATCATCTGATGGAATGTCTATTGCTCTACATAGCACCATGGTTTTCAAAGATTTCACTCAAAATCACTCTGCTGCACTTGCCCGTGCATTTTGCTCTTCCCTTAGGGGCCACCTAAAGAAAGCTTTGTCGGAATTTGGTGCGGCTTCAGGGTCTTTCTTACTGGAACCTAAGATGACTTCAGATGGTGGGATCTTTCCTTCACTGTTTCTCGTTGAGTTCCTGTTGTTTATTGCTGCCTCAAAGGAAAACCGCTGGGTAACTGCATTGCTTACAGAACTTGGAAATGATAGCAAGGATGTTCTGGAAGATATTGGGCGTGTCCATCGGGAAGTTCTATGGCAGATTGCTCTTCTAGAAGATACCAAGTCTGAAATGGAGGATGAAAGTGCTGGTTCTACCACTGAGTCGCAACAGTCAGAAATGAATGCAAGTGATTCTGAAGAGCAAAGGTTCAACTCTTTTAGGCAGTTTCTTGATCCATTGATGAGAAGAAGGCAATCTGGATGGAGCATTGAATCACAATTTCTTGATCTTATTAACCTGTATCGTGATCTCGGTCGCGCCACTAATTCTCAAAGAACACATTCTGATGGTCCGTCAAATCTGCGGATTGGATCTAGTCATCATTCTGGTTCTTCAGATGCTGGCAGAAGGGAGTATGACCAGCAGAGCTCCTACTATACTTCTTGCTGTGACATGGTAAGATCACTTTCTTTTGACATAAGTCATTTGTTTCAAGAGCTGGGAAAGGTCATGTACCAACCTTCTCGTCGACGGGATGATATTGTGAATGTTTCTGCTGCTGCAAAATCCGTGGCTTCTACATTTGCATCTATTGCATTGGATCACATGAGTTTTGGGGGGCATGCAAGTTCTTCTGGATCTGAGGTGTCGATATCAGCAAAATGTCGTTACTTTGGTAAAGTTATTGATTTCATTGATGTCAGTCTACTGGAGAGACCAGATTCATGTAACTCTGTTTTCCTCAATTGCTTGTATGGACATGGAGTTATCCAGTCAATTTTAACGACATTTGAAGCTACCAGTCAATTGCTCTTCACAGTAAATAGGGCTCCTGCATCTCCCATGGAAACTGATGATGGAAACTCAAAGCAGGATGAAAGGGAAGACACTGACCATTCATGGATTTATGGTCCGTTAGCTAGTTATGGAAAACTTATGGACCACCTGGTAACATCGTCTTTCATATTATCTCCTTTTACAAAACAGTTGCTTGCACAGCCCCTTGCAAATGGAACAATTCCTTTTCCACGCGATGCTGAGACCTTTGTAAAGGTCCTCCAATCCATGGTGCTGAAGGCAGTGCTTCCTGTTGGACTCATCCCCAATTTGCACATATACTCTGGGGTTGAAGTAAAGAATGTCAGTAGCAGTTCCAGTGCTCGTATTGCTGGCCCCCCTCCTAATGAGACAACTATATCAACGATTGTAGAGATGGGCTTCTCCAGGTCTAGGGCAGAAGAAGCTTTGAGACAGGTTGGATCCAATAGTGTGGAGTTGGCTATGGAGTGGTTGTGCTCCCATCAAGAGGAAGTTCAAGAAGACGATGAACTTGCTCGTGCACTTGCGATGTCTTTGGGTAATTCAGAGTCTGACACCAAGGAAGCTGGTGCAATGGAAAGTGCGCCGGAAGAAGAAAAGGTTCAACTTCCTCCTGTTGAGGAGTTGTTGTCTACATGCACAAAGCTTCTGCAGATGAAGGAACCTCTAGCTTTTCCAGTGCGAGACCTGCTTGTGATGATATGCTCCCAAAATGACGGTCAATGTAGGCCTAATATCATATCATTCATTGTTGATCGCGTCAAAGAATCTAGTTTGAATTCTGACAGTGGAAACAGTAACTTGCTATCTGCTCTTTTTCATGTTCTTGCTTTAATTCTTCATGAAGATGCAGTTGCAAGAGAAATAGCCTCGAAGAATGGATTTGTGAAAATAGCTTCAGATCTACTTTCGCAATGGGACTCTAGTTCAACTGGCATAGCAAAACGAGAAGTGCCCAAGTGGGTAACTACAGCTTTTCTTGCAATAGATCGGCTGTTGCAGGTGGATCAAAAATTGAATTCTGAAATTGCCGAGCAGTTGAAGAGAGATGCTGTTAGTAGCCAGCAAACATCTATTAGCATTGATGAGGACAAGCAAAATAAGTTGCAGTCTGCATTGGGATTATCTTCAAAGCACATAGAAATTAAGGAGCAGAAGAGGCTTATTGAGATTGCATGTTCATGTATCAGGAATCAGCTTCCTTCTGACACAATGCATGCAGTTCTGCAGCTGTGCTCTACCCTCACCAAAACACATACAGTTGCTGTTAGTTTTCTTGATGCTGGGGGTTTAAGTTTGCTACTCTCTTTGCCAACAAGTAGTCTATTTTCTGGTTTTGACAATATTGCTGCAACCATTATCCGTCATGTTCTTGAGGATCCTCAAACCCTTCAGCAGGCAATGGAATTTGAAATAAGGCACAATCTTGTGGCTGCTGCCAACAGACATTCAAATGGAAGGGTTTCTCCCCGCAATTTTCTAGCGAGTTTAAGTTCTGCCATTTCCAGGGATCCAGTGATATTCATGCGTGCTGCTCAGTCTATTTGTCAAGTGGAGATGGTTGGTGAGAGGCCTTGCATTGTTTTGTTGAAAGATCGTGACAAAGATAAAACCAAAGAGAAAGAAAAAGAGAAGGATAAATCTTTGGACAAGGAGAAAACACATATTTCGGATGGGAAAACTGCTCTGGGTAATACAAATCCAGTACCTTCTGGCAATGCACAAGGCAAGGCTCATGATTCAAACTCAAAGAGTGGTAAAGTTCATCGCAAATATCCCCAGAGTTTTGTGAGTGTGATTGAGCTTCTTCTAGATTCTGTGTATACTTATGTTCCTCCTTCAAAAGACAATCTTGTTGTTGATGTGCTCCATGATACCCCTTCATCGACTGACATGGACATTGATGTTGCTGCTGTCAAAGGAAAAGGAAAAGCTATTGCCTCATCTTCTGAAGATGGTGGAGCTGGTGCCCAAGAAGCTTCTGCATCACTCGCAAAGGTTGTTTTTATTTTGAAGCTTCTGACAGAGATTCTAGCAATGTATGCTTCATCTGCCCATGTTCTACTTCGGAAAGACGCTGAAATTAGTAGTTGCAGGAACCCTAATCCAAAGGGTTCTACTGCTGTATGCACTGGTGGAATATTTCACCATGTTCTTCACAAGTTTATTCCATATTCGCGGAGTGCCAAGAAGGAAAAGAAAACAGATGGTGATTGGAAGCACAAGTTAGCAAGCAGGGCGAGCCAGTTTTTGGTTGCATCATGTGTTCGTTCTTCCGAGGCGAGGAAGAGGATTTTTACAGAGATCAGTTATATTTTCAATGACTTTGTGGACTCATGTAATGGCTTTCGACCACCTGAAAATGACATACAAGCTTTTGCTGATCTGCTTAATGATGTTCTGGCTGCTCGGACTCCTACTGGTTCATACATCTCCGCAGAAGCGTCTGCTACTTTTATAGATGTTGGTCTGGTTGGATCATTGACTCGAACCCTCCAAGTGCTGGACCTGGACCATGCTGACTCGCCTAAAGTTGTTACGGGTCTGATCAAAGCTCTAGAACTGGTAACCAAAGAACATGTCCATTTGGCTGATTCAACTGCTGGTAAGGGCGACAGTTCTACAAAACCTCCTGATCAGTCTGGCAGGACAGATAATGTTGACGGTGGATCCCAGTCCATGGAGACACCATCCCAATCCCATCACAATTCTGCTCCAGCTGAGCGCACTGAGTCTTTTAATGCTGTCCAATCTTATGGTGGTTCTGAGGCTGTCACAGATGATATGGAACATGACCAAGATCTTGAAGGACGTTTTGCTGCTGCTAATGAAGATGAGTATATGCATGAAATTTCAGAGGATACTCGGGGTCTGGAAAACGGCATTGATACAATGGGTATACGGTTTGAAATTCAGCCTCATGAGCAAGAAAATCTTGAGGATGATGATGAGGATGATGAAGATATGTCTGAGGATGATGGAGATGAAGTGGATGATGATGAAGATGAAGATGATGAGGAGCATAATGATCTGGAAGATGAAGTCCATCACTTGCCACATCCTGACACTGATCAAGATGATCATGAGATTGATGACGAAGAGTTTGATGAAGAAGTATTAGAAGAAGATGATGAGGATGAGGAGGATGAGGAGGATGGAGTTATACTGAGGCTGGAGGAAGGCATAAATGGGATTAATGTTTTTGACCATATTGAGGTTTTTGGAAGAGAGCATGGTTTCCCTAATGAAACACTTCATGTGATGCCAGTGGAAGTTTTTGGGTCCAGACGTCAAGGGCGCACTACTTCTATTTACAGTCTTTTGGGCAGAACAGGTGAGAATGCGGCTCCATCAAGGCATCCTCTTTTGGTTGGACCTTTGTCTCTGCATTCGGCCCCTCCTAGGCAACCAGAAAATTCTCGTGATGGCATCATACCAGATAGGAACTCTGAGATCACCTCATCAAGATTGGACAGTATTTTCCGATCATTGAGGAATGGGCGCCATGGACACCGTCTGAACTTGTGGATGGATGATAACCAGCAAGGTGGTGGATCAAATGCCGGTGTAGTACCCCAAGGACTTGAGGAGTTGCTTGTTTCCCAATTGAGGCGACCAACACCCGAAAAGACTTCTGAAGAGAATACGACACCAGTAGATTCTCAGAACAAAGAAGTAGTTGAGTTGCAAGAACCGGATGCAGCTGTAAGGCCTGAAATGCCTGTTGAAAATGATATAAATAATGATAGTGCTAATGTGCCCCCTGCTGATACAATGGTCAACTCTGGCAATGCTGATTTGGTAACACCAACAGCAAGTGAATCTTTACAAGCAATGGACATGTCTAGCACACATCCACAGTCTGTTGAGATGCAGTTTGAACACACTGATGCAGCTGTGCGGGATGTAGAAGCTGTGAGCCAAGAAAGTGGTGGAAGTGGAGCAACTTTAGGGGAGAGCCTTCGTAGCCTTGATGTTGAAATTGGGAGTGCTGATGGTCATGATGATGGTGCAGATAGGCAAGGATCCTCAGATAGAATGCCACTTGGTGATTCTCAGGCGACTCGAGCGAGGAGAACGAACATATCTTTTGGGAATTCTGCATCTGCAAATGCAAGAGATGTTTCACTTCATAGTGTAACTGAGGTTTCAGAAAATTCTAGCCGGGAAGCAGATCAGGATGGTCCAGCAGCAGAACAGCAACTTAATAGTGATGCTGGTTCTGGGGCAATTGATCCTGCTTTCTTGGATGCTCTTCCTGAGGAGTTGCGAGCTGAAGTCCTCTCTGCACAACAGGGTCAAGTAGCTCCACAGTCAGCCTCTGAGCCACAAAATGCTGGTGATATTGATCCAGAATTCCTTGCTGCCCTTCCCCCAGATATCAGGGCTGAAGTTTTAGCTCAACAGCAAGCCCAAAGGGTACATCAGTCTCAGGAACTGGAAGGTCAACCAGTTGAAATGGACACTGTTTCAATAATTGCAACCTTTCCTTCAGATTTGAGAGAAGAGGTTCTGTTAACTTCATCTGATGCTATACTTGCCAATCTTACACCGGCTCTAGTTGCGGAGGCAAACATGTTGCGCGAAAGATTTGCCAATCGTTATAATCGTACCCTATTTGGTATGTATCCTAGAAACCGTAGGGGTGAGGCGTCAAGACCGGGCATTGGATCCAGCCTGGACAGAATCGGTGGAGGCCTTGCCTCACGTAGGCCTATAGGTGCTAAGGTAGTTGAAGCTGAAGGAGCTCCTTTAGTCGATGCAGAAGCTCTGCATGCAATGATTCGAGTGCTTCGAATGTTTCAGCCTCTATATAAAGGTCAGCTGCAGAAGCTTCTGTTGAATTTATGTGCTCATGTTGAAAGCAGAAATTCTGTTGTGAGAATTCTGATGGGCATACTAATGCTTGATACAAGAAAGCCTGGGAATCACTCAACTGATATCGAAACATCGTATCGGCTTTATGGTTGCCAGGGCAATGTGATGTATTCACGCGCTTCAGGTAAAGATGGAGTTCCACCATTGTTATCTCGCCGCATTCTGGAAACTCTGACATATTTGGCTCGGCATCATCCTAACGTGGCAAAGATTTTGCTTCATTTAAGGTTGCCTCATTCTGCTCTTCAAGAAAGAGAGAATAGTGATAGGGCTCGTGGAAAAGCTGTGATGGTTGTGGAGGAGAATGGTCAAAATAATCAGGAAGGATATCTGTCCATAGCCTTGCTTTTGAGCCTCTTGAATCAACCCCTTTATTTGACGAGAAGTATAGCTCATCTTGAACAGCTACTTAACTTATTGGATGTCATCATTGATAATGCTGAAACCAAGTTACCGGAAAAATCTGGGGTTTCTATTACTGAACAACCCTCGGCTCCTCAAGCTTCTGATGCTGAGATGAATACAGAGTCTGGTGGCATGTCCGTTGATGTTGGAACGCAAAGTAAGGTTGTTGATTCCTCCAAACCCTCCACTTCTGGTGCAGATGACGAATGCCATACTGAAAGTGTTCTGCTGAACCTGCCACAAGCAGAACTTCGCCTTCTATGTTCATTGCTTGCACGAGAAGGGCTTTCAGATAATGCTTATACTCTTGTGGCTGAGGTAATGAAGAAGTTGGTGGCAATTGTTCCTACCCATTCCAATCTGTTTATTACCGAGCTAGCAGATGCTGTTAGAAATTTGACTAGATCTGCCATGGATGAGTTGCGCAAGTTTGGTGAAGCAGTGAAAGCGCTCCTAAGTACCAAGTCGTCTGATGGAGCTGCTATTTTAAGAGTTCTGCAGGCATTAAGCTCCCTTGTTGCCTCACTGATTGAGAAAGAGAAGGATCCACAGATTCTTGCAGATAAGGAGCACACTACTCTTTCTCAGGTTTGGGATATCAATGCAGCTTTGGAGCCTTTGTGGCTTGAGCTAAGCAGTTGCATAAGTAAGATAGAAAGCTATTCAGAAACCACCCCTGATTTGTTGACTTCATATAGAGCTTCCACATCTAAACCATCTGGTGTAATTCCTCCCCTGCCTGCTGGGACTCAAAATATTTTGCCATACATAGAATCTTTCTTTGTGGTTTGTGAGAAATTGCATCCTGGGCAGCCAGGCCCTGGTAACGACTTCAGCGTTGCTGCAGTTTCTGATGTTGATGATGCCAGTACATCTGCTAGCCTGCAGAAAACCTCGGGTCCCACTGTGAAAGTGGATGAGAAACATGTTGCTTTTATGAAGTTCTCAGAGAAACATAGGAAGTTGCTAAATGCTTTCATCCGTCAAAATCCTGGTTTGCTAGAAAAGTCTTTCTCGCTGATGCTGAAGGTTCCTCGCTTTATTGATTTTGACAACAAACGTGCTCACTTCAGGTCAAAGATTAAGCATCAACATGATCATCATCACAATCCTCTAAGAATTTCTGTAAGAAGAGCATACATTCTTGAAGATTCATATAACCAGCTTCGCATGCGGTCAGCTCTAGATTTGAAGGGGAGATTGACTGTTCACTTTCAAGGAGAGGAAGGAATTGATGCTGGTGGACTCACGAGGGAATGGTATCAGTTGCTGTCCAGGGTTATCTTTGACAAGGGAGCACTACTATTTACAACAGTGGGCAATGAATCAACTTTCCAACCCAACCCTAACTCTGTTTACCAGACAGAACATCTTTCATATTTCAAGTTTGTTGGGCGAGTGGTTGGGAAAGCACTTTTCGATGGTCAATTATTGGATGTCCATTTTACTCGATCCTTCTACAAGCACATTTTGGGGGCTAAGGTGACTTATCATGATATTGAGGCCATTGATCCTGATTACTTTAAAAACCTCAAGTGGATGCTCGAGAATGATATAAGTGATGTTCTAGACCTTACTTTCAGTATTGATGCAGATGAGGAGAAACTAATATTATATGAAAGAACAGAAGTAACTGACTATGAACTGATTCCTGGAGGGCAAAACATTAAAGTTACAGAAGAAAATAAGCACCAATATGTTGACTTAGTTGCGGAGCATCGATTAACAACTGCTATTCGTCCCCAGATAAATGCGTTTTTGGATGGATTCTCTGAATTAATTCCTAGGGAACTAATATCCATTTTCAATGACAAGGAACTGGAACTATTGATCAGTGGGCTTCCAGATATTGACTTGGATGACATGAGGGCAAATACCGAATATTCTGGATACAGCGCTGGATCTCCTGTGATACAGTGGTTTTGGGAGGTTGTCCAAGCTCTTAGTAAGGAGGATAAAGCTCGACTATTGCAGTTTGTGACTGGCACCTCAAAGGTACCTTTAGAAGGTTTTAGTGCACTTCAGGGGATTTCAGGCTCACAGAAGTTCCAGATACACAAAGCATACGGAAGCCCTGATCACTTGCCATCTGCACACACTTGTTTCAATCAGTTGGATTTACCAGAGTATCCTTCTAAGCAACATCTTGAAGAGAGATTGCTTCTTGCGATTCACGAAGCCAATGAGGGATTTGGTTTTGGATAA

Protein Analysis

3645

Amino Acids

400.62

Weight (kDa)

5.11

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF908 PF06012 107 - 410 1.3e-31 Domain of Unknown Function (DUF908)
DUF913 PF06025 473 - 792 2.4e-56 Domain of Unknown Function (DUF913)
UBA PF00627 1271 - 1308 3.1e-09 UBA/TS-N domain
UBA_7 PF22562 1272 - 1318 3.9e-14 UBA-like domain
UBM PF14377 2561 - 2584 3.3e-07 Ubiquitin binding region
UBM PF14377 2595 - 2623 3e-11 Ubiquitin binding region
UBM PF14377 2632 - 2663 5.8e-07 Ubiquitin binding region
HECT PF00632 3334 - 3644 3.1e-99 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 782, 8055
AarI CACCTGC 1 cut(s) 4441
AasI GACNNNNNNGTC 1 cut(s) 253
AatII GACGTC 1 cut(s) 6742
Acc16I TGCGCA 1 cut(s) 9158
Acc36I ACCTGC 4 cut(s) 921, 4146, 4441, 8976
Acc65I GGTACC 2 cut(s) 856, 10729
AccB1I GGYRCC 5 cut(s) 856, 5417, 6951, 10718, 10729
AccB7I CCANNNNNTGG 6 cut(s) 2278, 3697, 5701, 5953, 6130, 9554
AccBSI CCGCTC 1 cut(s) 1147
AccI GTMKAC 5 cut(s) 3160, 3337, 4082, 5273, 6349
AccII CGCG 6 cut(s) 2949, 3663, 4209, 5629, 8034, 8445
AclI AACGTT 1 cut(s) 1740
AcyI GRCGYC 3 cut(s) 6739, 6952, 8102
AdeI CACNNNGTG 1 cut(s) 7499
AfeI AGCGCT 2 cut(s) 9183, 10630
AflIII ACRYGT 4 cut(s) 6028, 7290, 8025, 9658
AjuI GAANNNNNNNTTGG 4 cut(s) 1804, 1836, 1955, 1987
AleI CACNNNNGTG 1 cut(s) 1903
Alw44I GTGCAC 2 cut(s) 3969, 10749
Ama87I CYCGRG 5 cut(s) 65, 6315, 7515, 9628, 10277
Aor51HI AGCGCT 2 cut(s) 9183, 10630
ApaLI GTGCAC 2 cut(s) 3969, 10749
ArsI GACNNNNNNTTYG 6 cut(s) 4153, 4185, 6015, 6047, 6536, 6568
AseI ATTAAT 2 cut(s) 6645, 10511
Asp718I GGTACC 2 cut(s) 856, 10729
AspA2I CCTAGG 2 cut(s) 6851, 10517
AsuC2I CCSGG 2 cut(s) 7616, 8112
AsuII TTCGAA 1 cut(s) 8245
AsuNHI GCTAGC 3 cut(s) 2167, 9109, 9611
AvaI CYCGRG 5 cut(s) 65, 6315, 7515, 9628, 10277
AvrII CCTAGG 2 cut(s) 6851, 10517
AxyI CCTNAGG 2 cut(s) 2489, 7710
BaeGI GKGCMC 6 cut(s) 1153, 3973, 4407, 5422, 7212, 10753
BaeI ACNNNNGTAYC 8 cut(s) 7212, 7245, 7862, 7895, 10637, 10670, 10844, 10877
BamHI GGATCC 7 cut(s) 3890, 4828, 4960, 6119, 7475, 8120, 9277
BanI GGYRCC 5 cut(s) 856, 5417, 6951, 10718, 10729
BanII GRGCYC 5 cut(s) 1117, 3062, 3463, 8200, 8602
BauI CACGAG 6 cut(s) 903, 3966, 6870, 8601, 9009, 9982
BbsI GAAGAC 4 cut(s) 1602, 1835, 3522, 3957
BbvCI CCTCAGC 1 cut(s) 9048
BceAI ACGGC 4 cut(s) 10, 55, 79, 6346
BcgI CGANNNNNNTGC 4 cut(s) 3944, 3978, 8024, 8058
BciVI GTATCC 4 cut(s) 6304, 8088, 10616, 10872
BclI TGATCA 8 cut(s) 2245, 5997, 6085, 6520, 6529, 9829, 10563, 10810
BcnI CCSGG 2 cut(s) 7616, 8112
BfoI RGCGCY 3 cut(s) 6955, 9185, 10632
BfuAI ACCTGC 4 cut(s) 921, 4146, 4441, 8976
BfuI GTATCC 4 cut(s) 6304, 8088, 10616, 10872
BglI GCCNNNNNGGC 1 cut(s) 9355
BglII AGATCT 5 cut(s) 793, 1682, 4351, 6250, 9137
BlnI CCTAGG 2 cut(s) 6851, 10517
BlpI GCTNAGC 2 cut(s) 6171, 9365
BmcAI AGTACT 2 cut(s) 327, 763
BmeT110I CYCGRG 5 cut(s) 65, 6315, 7515, 9628, 10277
BmrI ACTGGG 1 cut(s) 6118
BmtI GCTAGC 3 cut(s) 2171, 9113, 9615
BmuI ACTGGG 1 cut(s) 6118
BoxI GACNNNNGTC 2 cut(s) 3111, 6829
BpiI GAAGAC 4 cut(s) 1602, 1835, 3522, 3957
BplI GAGNNNNNCTC 2 cut(s) 6893, 6925
BpmI CTGGAG 7 cut(s) 1202, 1567, 3362, 3838, 6150, 6644, 10407
Bpu10I CCTNAGC 3 cut(s) 8175, 9048, 10212
Bpu1102I GCTNAGC 2 cut(s) 6171, 9365
Bpu14I TTCGAA 1 cut(s) 8245
BpuEI CTTGAG 6 cut(s) 4844, 6407, 7055, 8811, 9380, 10250
BpuMI CCSGG 2 cut(s) 7616, 8112
Bsa29I ATCGAT 1 cut(s) 10453
BsaAI YACGTR 1 cut(s) 8159
BsaBI GATNNNNATC 3 cut(s) 1386, 7797, 9834
BsaHI GRCGYC 3 cut(s) 6739, 6952, 8102
BsaI GGTCTC 4 cut(s) 1925, 3340, 3665, 4128
BsaWI WCCGGW 3 cut(s) 167, 7144, 8782
Bse118I RCCGGY 2 cut(s) 7013, 8002
Bse21I CCTNAGG 2 cut(s) 2489, 7710
Bse8I GATNNNNATC 3 cut(s) 1386, 7797, 9834
BseCI ATCGAT 1 cut(s) 10453
BseJI GATNNNNATC 3 cut(s) 1386, 7797, 9834
BseSI GKGCMC 6 cut(s) 1153, 3973, 4407, 5422, 7212, 10753
BseYI CCCAGC 7 cut(s) 177, 561, 1874, 2640, 3130, 4733, 9476
BsgI GTGCAG 5 cut(s) 2445, 7482, 7722, 8949, 10809
Bsh1236I CGCG 6 cut(s) 2949, 3663, 4209, 5629, 8034, 8445
Bsh1285I CGRYCG 2 cut(s) 1061, 2947
BshNI GGYRCC 5 cut(s) 856, 5417, 6951, 10718, 10729
BshVI ATCGAT 1 cut(s) 10453
BsiEI CGRYCG 2 cut(s) 1061, 2947
BsiHKCI CYCGRG 5 cut(s) 65, 6315, 7515, 9628, 10277
BsiSI CCGG 8 cut(s) 168, 4037, 7014, 7145, 7615, 8003, 8111, 8783
BslFI GGGAC 4 cut(s) 4382, 9493, 9618, 10458
BsmBI CGTCTC 1 cut(s) 102
BsmFI GGGAC 4 cut(s) 4382, 9493, 9618, 10458
BsmI GAATGC 9 cut(s) 727, 1690, 2311, 2818, 6796, 6838, 7494, 8487, 8945
Bso31I GGTCTC 4 cut(s) 1925, 3340, 3665, 4128
BsoBI CYCGRG 5 cut(s) 65, 6315, 7515, 9628, 10277
Bsp119I TTCGAA 1 cut(s) 8245
Bsp1407I TGTACA 1 cut(s) 2383
Bsp1720I GCTNAGC 2 cut(s) 6171, 9365
Bsp19I CCATGG 6 cut(s) 2424, 3474, 3696, 6129, 6955, 9144
BspDI ATCGAT 1 cut(s) 10453
BspFNI CGCG 6 cut(s) 2949, 3663, 4209, 5629, 8034, 8445
BspHI TCATGA 8 cut(s) 1141, 1696, 4291, 5185, 6370, 6532, 7450, 10225
BspMAI CTGCAG 8 cut(s) 932, 2230, 4104, 4680, 8280, 9234, 9580, 9621
BspMI ACCTGC 4 cut(s) 921, 4146, 4441, 8976
BspOI GCTAGC 3 cut(s) 2171, 9113, 9615
BspQI GCTCTTC 8 cut(s) 2058, 2488, 2742, 2823, 3449, 7710, 8577, 9859
BspT104I TTCGAA 1 cut(s) 8245
BspT107I GGYRCC 5 cut(s) 856, 5417, 6951, 10718, 10729
BspTNI GGTCTC 4 cut(s) 1925, 3340, 3665, 4128
BsrBI CCGCTC 1 cut(s) 1147
BsrFI RCCGGY 2 cut(s) 7013, 8002
BsrGI TGTACA 1 cut(s) 2383
BssAI RCCGGY 2 cut(s) 7013, 8002
BssNAI GTATAC 2 cut(s) 5274, 6350
BssNI GRCGYC 3 cut(s) 6739, 6952, 8102
BssSI CACGAG 6 cut(s) 903, 3966, 6870, 8601, 9009, 9982
Bst1107I GTATAC 2 cut(s) 5274, 6350
Bst2BI CACGAG 6 cut(s) 903, 3966, 6870, 8601, 9009, 9982
BstACI GRCGYC 3 cut(s) 6739, 6952, 8102
BstAPI GCANNNNNTGC 4 cut(s) 948, 1103, 3224, 5492
BstAUI TGTACA 1 cut(s) 2383
BstBAI YACGTR 1 cut(s) 8159
BstBI TTCGAA 1 cut(s) 8245
BstDSI CCRYGG 7 cut(s) 2424, 3201, 3474, 3696, 6129, 6955, 9144
BstEII GGTNACC 1 cut(s) 6016
BstENI CCTNNNNNAGG 4 cut(s) 2205, 4844, 8158, 10737
BstFNI CGCG 6 cut(s) 2949, 3663, 4209, 5629, 8034, 8445
BstH2I RGCGCY 3 cut(s) 6955, 9185, 10632
BstMCI CGRYCG 2 cut(s) 1061, 2947
BstPAI GACNNNNGTC 2 cut(s) 3111, 6829
BstPI GGTNACC 1 cut(s) 6016
BstSLI GKGCMC 6 cut(s) 1153, 3973, 4407, 5422, 7212, 10753
BstUI CGCG 6 cut(s) 2949, 3663, 4209, 5629, 8034, 8445
BstV2I GAAGAC 4 cut(s) 1602, 1835, 3522, 3957
BstXI CCANNNNNNTGG 5 cut(s) 1951, 3901, 6206, 6998, 7379
BstZ17I GTATAC 2 cut(s) 5274, 6350
Bsu15I ATCGAT 1 cut(s) 10453
Bsu36I CCTNAGG 2 cut(s) 2489, 7710
BsuI GTATCC 4 cut(s) 6304, 8088, 10616, 10872
BsuTUI ATCGAT 1 cut(s) 10453
BtgI CCRYGG 7 cut(s) 2424, 3201, 3474, 3696, 6129, 6955, 9144
BtgZI GCGATG 3 cut(s) 3678, 3992, 5201
BtsI GCAGTG 2 cut(s) 3717, 9180
BveI ACCTGC 4 cut(s) 921, 4146, 4441, 8976
CciI TCATGA 8 cut(s) 1141, 1696, 4291, 5185, 6370, 6532, 7450, 10225
Cfr10I RCCGGY 2 cut(s) 7013, 8002
ClaI ATCGAT 1 cut(s) 10453
CseI GACGC 4 cut(s) 4198, 5534, 5883, 8091
CsiI ACCWGGT 1 cut(s) 3576
CspCI CAANNNNNGTGG 4 cut(s) 2040, 2075, 3183, 3218
DinI GGCGCC 1 cut(s) 6953
DraI TTTAAA 3 cut(s) 822, 1474, 10258
DraIII CACNNNGTG 1 cut(s) 7499
DrdI GACNNNNNNGTC 1 cut(s) 253
DseDI GACNNNNNNGTC 1 cut(s) 253
EciI GGCGGA 1 cut(s) 40
Ecl136II GAGCTC 3 cut(s) 1115, 3060, 8198
Eco147I AGGCCT 5 cut(s) 4180, 5023, 7162, 8148, 8164
Eco24I GRGCYC 5 cut(s) 1117, 3062, 3463, 8200, 8602
Eco31I GGTCTC 4 cut(s) 1925, 3340, 3665, 4128
Eco32I GATATC 5 cut(s) 3285, 7831, 8392, 8657, 9331
Eco47III AGCGCT 2 cut(s) 9183, 10630
Eco53kI GAGCTC 3 cut(s) 1115, 3060, 8198
Eco81I CCTNAGG 2 cut(s) 2489, 7710
Eco88I CYCGRG 5 cut(s) 65, 6315, 7515, 9628, 10277
Eco91I GGTNACC 1 cut(s) 6016
EcoICRI GAGCTC 3 cut(s) 1115, 3060, 8198
EcoNI CCTNNNNNAGG 4 cut(s) 2205, 4844, 8158, 10737
EcoO109I RGGNCCY 3 cut(s) 3685, 9551, 9632
EcoO65I GGTNACC 1 cut(s) 6016
EcoRI GAATTC 5 cut(s) 4228, 4468, 7546, 7805, 8334
EcoRV GATATC 5 cut(s) 3285, 7831, 8392, 8657, 9331
EcoT22I ATGCAT 3 cut(s) 498, 4668, 6297
EcoT38I GRGCYC 5 cut(s) 1117, 3062, 3463, 8200, 8602
EgeI GGCGCC 1 cut(s) 6953
EheI GGCGCC 1 cut(s) 6953
Esp3I CGTCTC 1 cut(s) 102
FaqI GGGAC 4 cut(s) 4382, 9493, 9618, 10458
FauI CCCGC 3 cut(s) 1140, 4928, 7344
FauNDI CATATG 1 cut(s) 1660
FbaI TGATCA 8 cut(s) 2245, 5997, 6085, 6520, 6529, 9829, 10563, 10810
FblI GTMKAC 5 cut(s) 3160, 3337, 4082, 5273, 6349
FriOI GRGCYC 5 cut(s) 1117, 3062, 3463, 8200, 8602
FspI TGCGCA 1 cut(s) 9158
GsaI CCCAGC 7 cut(s) 181, 565, 1878, 2644, 3134, 4737, 9480
GsuI CTGGAG 7 cut(s) 1202, 1567, 3362, 3838, 6150, 6644, 10407
HaeII RGCGCY 3 cut(s) 6955, 9185, 10632
HapII CCGG 8 cut(s) 168, 4037, 7014, 7145, 7615, 8003, 8111, 8783
HgaI GACGC 4 cut(s) 4198, 5534, 5883, 8091
Hin1I GRCGYC 3 cut(s) 6739, 6952, 8102
HincII GTYRAC 8 cut(s) 1993, 3161, 6112, 7231, 7898, 7968, 9420, 10435
HindII GTYRAC 8 cut(s) 1993, 3161, 6112, 7231, 7898, 7968, 9420, 10435
HpaI GTTAAC 1 cut(s) 7968
HpaII CCGG 8 cut(s) 168, 4037, 7014, 7145, 7615, 8003, 8111, 8783
Hpy99I CGWCG 3 cut(s) 59, 3162, 3165
HpyCH4IV ACGT 6 cut(s) 1740, 6262, 6739, 8158, 8529, 9793
HpySE526I ACGT 6 cut(s) 1740, 6262, 6739, 8158, 8529, 9793
Hsp92I GRCGYC 3 cut(s) 6739, 6952, 8102
KasI GGCGCC 1 cut(s) 6951
KflI GGGWCCC 1 cut(s) 9632
KpnI GGTACC 2 cut(s) 860, 10733
Ksp22I TGATCA 8 cut(s) 2245, 5997, 6085, 6520, 6529, 9829, 10563, 10810
KspAI GTTAAC 1 cut(s) 7968
LguI GCTCTTC 8 cut(s) 2058, 2488, 2742, 2823, 3449, 7710, 8577, 9859
MabI ACCWGGT 1 cut(s) 3576
MaeII ACGT 6 cut(s) 1740, 6262, 6739, 8158, 8529, 9793
MbiI CCGCTC 1 cut(s) 1147
MfeI CAATTG 5 cut(s) 3376, 3437, 7055, 7680, 9072
Mly113I GGCGCC 1 cut(s) 6952
Mph1103I ATGCAT 3 cut(s) 498, 4668, 6297
MspA1I CMGCKG 8 cut(s) 177, 1460, 2640, 4681, 6170, 7154, 7346, 8275
MspI CCGG 8 cut(s) 168, 4037, 7014, 7145, 7615, 8003, 8111, 8783
MunI CAATTG 5 cut(s) 3376, 3437, 7055, 7680, 9072
Mva1269I GAATGC 9 cut(s) 727, 1690, 2311, 2818, 6796, 6838, 7494, 8487, 8945
MvnI CGCG 6 cut(s) 2949, 3663, 4209, 5629, 8034, 8445
NarI GGCGCC 1 cut(s) 6952
NciI CCSGG 2 cut(s) 7616, 8112
NcoI CCATGG 6 cut(s) 2424, 3474, 3696, 6129, 6955, 9144
NdeI CATATG 1 cut(s) 1660
NheI GCTAGC 3 cut(s) 2167, 9109, 9611
NmeAIII GCCGAG 3 cut(s) 4507, 8494, 8798
NmuCI GTSAC 7 cut(s) 1757, 1777, 3080, 5048, 6223, 10216, 10711
NsbI TGCGCA 1 cut(s) 9158
NsiI ATGCAT 3 cut(s) 498, 4668, 6297
NspV TTCGAA 1 cut(s) 8245
OliI CACNNNNGTG 1 cut(s) 1903
PaeI GCATGC 4 cut(s) 3257, 4670, 8228, 9907
PaeR7I CTCGAG 2 cut(s) 7515, 10277
PagI TCATGA 8 cut(s) 1141, 1696, 4291, 5185, 6370, 6532, 7450, 10225
PaqCI CACCTGC 1 cut(s) 4441
PceI AGGCCT 5 cut(s) 4180, 5023, 7162, 8148, 8164
PciI ACATGT 4 cut(s) 6028, 7290, 8025, 9658
PciSI GCTCTTC 8 cut(s) 2058, 2488, 2742, 2823, 3449, 7710, 8577, 9859
PcsI WCGNNNNNNNCGW 4 cut(s) 910, 8056, 8242, 9567
PctI GAATGC 9 cut(s) 727, 1690, 2311, 2818, 6796, 6838, 7494, 8487, 8945
PflFI GACNNNGTC 1 cut(s) 6963
PflMI CCANNNNNTGG 6 cut(s) 2278, 3697, 5701, 5953, 6130, 9554
PfoI TCCNGGA 2 cut(s) 29, 10384
PluTI GGCGCC 1 cut(s) 6955
Ppu21I YACGTR 1 cut(s) 8159
PpuMI RGGWCCY 2 cut(s) 3685, 9632
PscI ACATGT 4 cut(s) 6028, 7290, 8025, 9658
PshAI GACNNNNGTC 2 cut(s) 3111, 6829
PshBI ATTAAT 2 cut(s) 6645, 10511
PsiI TTATAA 2 cut(s) 782, 8055
Psp124BI GAGCTC 3 cut(s) 1117, 3062, 8200
Psp1406I AACGTT 1 cut(s) 1740
Psp5II RGGWCCY 2 cut(s) 3685, 9632
PspEI GGTNACC 1 cut(s) 6016
PspFI CCCAGC 7 cut(s) 177, 561, 1874, 2640, 3130, 4733, 9476
PspPPI RGGWCCY 2 cut(s) 3685, 9632
PspXI VCTCGAGB 1 cut(s) 7515
PsrI GAACNNNNNNTAC 2 cut(s) 1863, 1895
PstI CTGCAG 8 cut(s) 932, 2230, 4104, 4680, 8280, 9234, 9580, 9621
PsyI GACNNNGTC 1 cut(s) 6963
PvuII CAGCTG 6 cut(s) 1460, 4681, 6170, 7154, 7346, 8275
SacI GAGCTC 3 cut(s) 1117, 3062, 8200
SalI GTCGAC 1 cut(s) 3159
SapI GCTCTTC 8 cut(s) 2058, 2488, 2742, 2823, 3449, 7710, 8577, 9859
ScaI AGTACT 2 cut(s) 327, 763
SexAI ACCWGGT 1 cut(s) 3576
SfoI GGCGCC 1 cut(s) 6953
Sfr274I CTCGAG 2 cut(s) 7515, 10277
SfuI TTCGAA 1 cut(s) 8245
SgrDI CGTCGACG 1 cut(s) 3159
SlaI CTCGAG 2 cut(s) 7515, 10277
SmlI CTYRAG 8 cut(s) 4823, 6386, 7034, 7515, 8826, 9359, 10265, 10277
SmoI CTYRAG 8 cut(s) 4823, 6386, 7034, 7515, 8826, 9359, 10265, 10277
SphI GCATGC 4 cut(s) 3257, 4670, 8228, 9907
SseBI AGGCCT 5 cut(s) 4180, 5023, 7162, 8148, 8164
SspDI GGCGCC 1 cut(s) 6951
SspI AATATT 6 cut(s) 1336, 4795, 5592, 9490, 10344, 10616
SstI GAGCTC 3 cut(s) 1117, 3062, 8200
StuI AGGCCT 5 cut(s) 4180, 5023, 7162, 8148, 8164
TaiI ACGT 6 cut(s) 1743, 6265, 6742, 8161, 8532, 9796
TaqII GACCGA 1 cut(s) 2933
TatI WGTACW 8 cut(s) 183, 325, 731, 761, 1213, 1605, 2383, 9602
TauI GCSGC 4 cut(s) 217, 2531, 6797, 8487
TseFI GTSAC 7 cut(s) 1757, 1777, 3080, 5048, 6223, 10216, 10711
Tsp45I GTSAC 7 cut(s) 1757, 1777, 3080, 5048, 6223, 10216, 10711
TspGWI ACGGA 8 cut(s) 902, 2973, 3190, 3534, 4802, 8860, 9704, 10815
Tth111I GACNNNGTC 1 cut(s) 6963
Van91I CCANNNNNTGG 6 cut(s) 2278, 3697, 5701, 5953, 6130, 9554
VneI GTGCAC 2 cut(s) 3969, 10749
VspI ATTAAT 2 cut(s) 6645, 10511
XagI CCTNNNNNAGG 4 cut(s) 2205, 4844, 8158, 10737
XbaI TCTAGA 5 cut(s) 2740, 5260, 6007, 9916, 10300
XcmI CCANNNNNNNNNTGG 5 cut(s) 2758, 6206, 6998, 7379, 9643
XhoI CTCGAG 2 cut(s) 7515, 10277
XmaJI CCTAGG 2 cut(s) 6851, 10517
XmiI GTMKAC 5 cut(s) 3160, 3337, 4082, 5273, 6349
ZraI GACGTC 1 cut(s) 6740
ZrmI AGTACT 2 cut(s) 327, 763
Zsp2I ATGCAT 3 cut(s) 498, 4668, 6297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.