Rroxscaffold_3G00226700

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012629
Physical Location & Seq
Reverse (-)
10329756 .. 10342309
12554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00226700.1

Sequence Viewer

Length: 9750 bp
ATGCCAGATCTGCATTTGCAGAAGGAGGATGATTTAAAGCTGATGAAACAGTGCATAGAGGACTACAGGGTACCTCCTGAGATTCGGTTTTCACTCCTGACAAGAATCAGATATGCTCGTGCCTTCCGTTCACCAAGAATCTGCAGGTTGTACAGCAGGATTTGCCTTCTTGCATTTATTGTGCTTGTTCAATCTAGTGATGCACATGAAGAACTTGTATCATTCTTTGCTAATGAGCCAGAATATACTAATGAACTGATTAGAATTGTGCGGTCGGAAGAATCAGTTTCTGGAACCATCAGAACACATGCAATGCTTGCCTTGGGAGCTCAATTAGCTGCTTATTCTGCATCTCATGAGCGGGCACGAATTTTGAGTGGATCTAGTATCAGTTTTGCAGGAGGGAATCGTATGATACTTCTGAATGTACTCCAGAGAGCAGTTTTATCACTTAAAACCTCCAATGATCCAACTTCTCTTGCTTTTGTTGAAGCTCTGCTTCAGTTCTATTTGCTCCATGTGGTTTCATCGTCCACTACAGGGAGTAATATTAGAGGGTCAGGCATGGTTCCAACATTCTTGCCTCTTTTGGAGGATTCTGACCCCTTGCACTTGCATCTAGTCTGTTTTGCTGTGAAAACTCTGCAAAAGCTCATGGATTACAGTAGTTCAGCTGTGTCTCTCTTTAAAGAGTTAGGGGAATATAATGAAGACCAGTTGTACTCCCAGAAGCGGCTCATTAAAGCTTCGTTGAAGGCACTTGGGTCTGCTTCATATGCTGCTGGCAATTCTACCAGATCTCAGCATCCTCATGACAGTTCTTTACCTGAAACTCTTTCTCTGATCTTTGGGAACGTTGATAAGTTTGGAGGTGACATTTATCACTCTGCTGTGACAGTTCTGAGTGAAACGATACACAAAGACCCAACTTGTTATTCTGCTTTGCACGATATGGGTCTTCCAGATGCTTTCATATCTTCAGTTGTAGCTGGGGTTCTTCCATCTGCAAAGGCACTTCAGTGTGTGCCTAATGGTCTCGGTGCTATATGTCTTAATGCCAAAGGCTTGGAGACTGTGAAGGAAAAGTTGGCCTTACGATTTCTTGTTGACATCTTCACCAGCAAGAAGTATGTAGTAGCAATGAATGGAGCTATTGTCCATTTGACAAATGCTGTGGAAGAGCTTTTGCGCCATGTGTCTTCGCTAAGAAGTATTGGTGTTGATATTATGATTGAAATTGTTGAAAAAATTGCTTCCTTCACTGACAACAACAATGCAGGGCTAGCAGGAAAGGCTAGTGGGAGTGGGAGTACTGCTATGGAAATGGATTCTGAAGACAAGGAACAGCCTTTGGTAGGTGCTGTAGATTCGGCTGCAGATGGTATCAGTAATGATCAGTTCATTCAATTATCCATTTTCCATTTGATGGTACTGGTCCACAGAACAATGGAGAATGCTGAAATATGTCGGTTATTTGTTGAAAAGTCTGGAATTGATGCTTTACTGAAGCTTTTGTTACAGCCAACTGTTGTACAATCATCTGATGGAATGTCTATTGCTCTACATAGCACCATGGTTTTCAAAGATTTCACTCAAAATCACTCTGCTGCACTTGCCCGTGCATTTTGCTCTTCCCTTAGGGGCCACCTAAAGAAAGCTTTGTTGGAATTTGGTGCGGCTTCAGGGTCTTTCTTACTGGAACCTAAGATGACTTCAGATGGTGGGATCTTTCCTTCACTGTTTCTCATTGAGTTCCTTTTGTTTATCGCTGCCTCAAAGGAAAACCGCTGGGTAACTGCATTGCTTACAGAACTTGGAAATGATAGCAAGGATGTTTTGGAAGATATTGGGCGTGTCCATCAGGAAGTTCTATGGCAGATTGCTCTTCTAGAAGATACCAAGTCTGAAATGGAGGATGAAAGTGCTGGTTCTACCACTGAGTCGCAACAGTCAGAAATGAATGCAAGTGATTCTGAAGAGCAAAGGTTCAACTCTTTTAGGCAGTTTCTTGATCCATTGATGAGAAGAAGGCAATCTGGATGGAGCATTGAATCGCAATTTCTTGATCTTATTAACCTGTATCGTGATCTCGGTCGCGCCACTAGTTCTCAAAGAACACATTCTGATGGTCCGTCAAATCTGCGGATTGGATCTAGTCATCATTCTGGTTCTTCAGATGCTGGTAGAAGGGAGTTTGACCAGCAGAGCTCCTACTATACTTCTTGCTGTGACATGGTAAGATCACTTTCTTTTGACATAAGTCATTTGTTTCAAGAGCTGGGAAAGGTCATGTACCAACCTTCTCGTCGACGGGATGATATTGTGAATGTTTCCGCTGCTGCAAAATCCGTGGCTTCTACATTTGCATCTATTGCATTTGATCACATGAGTTTTGAGGGGCATGCAAGTTCTTCTGGATCTGAGGTGTCTATATCAGCAAAATGTCGTTACTTTGGTAAAGTTATTGATTTCATTGATGTCAGTCTACTGGAGAGACCAGATTCATGTAACTCTGTTTTCCTCAATTGCTTGTATGGGCATGGAGTTATCCAGTCAGTTTTAACGACATTTGAAGCTACCAGTCAATTGCTCTTCACAGTAAATAGGGCTCCTGCATCTCCCATGGAAACTGATGATGGGAACTCAAAGCAGGATGAAAGGGAAGATACTGACCATTCATGGATTTATGGTCCGTTAGCTAGTTATGGAAAACTTATGGATCACCTGGTAACATCGTCTTTCATATTATCTCCTTTTACAAAACAGTTGCTTGCACAGCCCCTTGCAAATGGAACAATTCCTTTTCCACGTGATGCTGAGACCTTTGTAAAGGTCCTCCAATCCATGGTGCTGAAGGCAGTGCTTCCTGTCTGGACTCATCCCCAATTTGTCGACTGTAGTTATGATTTTATTACTACAGTTATTTCTATAATCAGGCACATATACTCTGGGGTTGAAGTAAAGAATGTCAGTAGCAGTTCCAGTGCCCGTATTGCTGGTCCCCCTCCTAATGAGACAACTATATCAACGATTGTAGAGATGGGCTTCTCCAGGTCTAGGGCAGAAGAAGCTTTGAGACAGGTTGGATCCAATAGTGTGGAGTTGGCTATGGAGTGGTTGTGCTCCCATCAAGAGGAAGTTCAAGAAGACGATGAACTTGCTCGTGCACTTGCCATGTCTTTGGGTAATTCAGAGTCTGACACTAAGGAAGCTGGTGCAATGGAAAGTGCGCCGGAAGAAGAAAAGGTTCAACTTCCTCCTGTTGAGGAGTTGTTGTCTACATGCACAAAGCTTCTGCAGATGAAGGAACCTCTAGCTTTTCCAGTGCGAGACCTGCTTGTGATGATATGCTCCCAAAATGACGGTCAATGTAGGCCTAATATCATATCATTCATTGTTGATCGTGTCAAAGAATCTAGTTTGAATTCTGACAGTGGAAACAGTAACTTGCTATCCGCTCTTTTTCATGTTCTTGCTTTAATTCTTCACGAAGATGCAGTTGCAAGAGAAATAGCCTCGAAGAATGGATTTGTGAAAATAGCTTCAGATCTACTTTCGCAATGGGACTCTAGTTCAACTGGCATAGCAAAACGAGAAGTGCCCAAGTGGGTAACTACAGCTTTTCTTGCAATAGATCGGCTGTTGCAGGTGGATCAAAAATTGAATTCTGAAATTGCTGAGCAGTTGAAGAGAGATGCTGTTAGTAGCCAGCAAACATCTATTAGCATTGATGAGGACAAGCAAAATAAGTTGCAGTCTGCATTGGGATTATCTTCGAAGCACATAGAAATTAAGGAGCAGAAGAGGCTTATTGAGATTGCTTGTTCATGTATCAGGAATCAGCTTCCTTCTGAAACAATGCATGCAGTTCTGCAGCTGTGCTCTACCCTCACCAAAACACATACAGTTGCTGTTAGTTTTCTTGATGCTGGGGGTTTAAGTTTGCTACTCTCTTTGCCAACAAGTAGTTTATTTTCTGGTTTTGACAATATTGCTGCAACCATTATCCGTCATGTTCTTGAGGATCCTCAAACCCTTCAGCAGGCAATGGAATTTGAAATAAGGCACAATCTTGTGGCTGCTGCCAACAGACATTCAAATGGAAGGGTTTCTCCCCGCAATTTTCTAGCAAGTTTAAGTTCTGCCATTTCCAGGGATCCAGTGATATTCATGCGTGCTGCTCAGTCTATTTGTCAAGTGGAGATGGTTGGTGAGAGGCCTTGCATTGTTTTGTTGAAAGATCGTGACAAAGATAAAACCAAAGAGAAAGAAAAAGAGAAGGATAAATCTTTGGACAAGGAGAAAACACATACTTCGGATGGGAAAACTGCTCTGGGTAATACAAATCCAGTACCTTCTGGCAATGCACAAGGCAAGGCTCATGATTCAAACTCAAAGAGTGGTAAAGTTCATCGCAAATATCCCCAGAGTTTTGTGAGTGTGATTGAGCTTCTTCTAGATTCTGTGTATACTTATGTTCCTCCTTCAAAAGACAATCTTGTTGTTGATGTGCTCCATGATACCCCGTCATCGACTGACATGGACATTGATGTTGCTGCTGTCAAAGGAAAAGGAAAAGCTATTGCCTCATCTTCTGAAGATGGTGGAGCTGGTGCCCAAGAAGCTTCTGCATCACTTGCAAAGGTGGTTTTTATTTTGAAGCTTCTGACAGAGATTCTAGCAATGTATGCTTCATCTGCCCATGTTCTACTTCGGAAAGACGCTGAAATTAGTAGTTGCAGGAACCCTAATCCAAAGGGTTCTGCTGTATGCACTGGTGGAATATTTCACCATGTTCTTCACAAGTTTATTCCATATTCGCGGAGTGCCAAGAAGGAAAAGAAAACAGATGGTGATTGGAAGCACAAGTTAGCAAGCAGGGCGAGCCAGTTTTTGGTTGCATCATGTGTTCGTTCTTCCGAGGCGAGGAAAAGGATTTTTACAGAGATCAGTTATATTTTCAATGACTTTGTGGACTCATGTAATGGCTTTCGACCACCTGAAAATGACATACAAGCTTTTGCTGATCTGCTTAATGATGTTCTGGCTGCTCGGACTCCTACTGGTTCATACATCTCCGCAGAAGCGTCTGCTACTTTTATAGATGTTGGTCTAGTTGGATCATTGACTCGAACCCTCCAAGTGCTGGACCTGGACCATGCTGACTCACCTAAAGTTGTTACAGGTCTGATCAAAGCTCTAGAACTGGTAACCAAAGAACATGTCCATTTGGCTGATTCAACTGCTGGTAAGGGCGACAGTTCTACAAAACCTCCTGATCAGTCTGGCGGGACAGATAATGTTGGCGGTGGATCCCAGTCCATGGAGACACCATCCCAATCCCATCACAATTCTGCTCCAGCTGAGCGCACTGAGTCTTTTAATGCTGTCCAATCTTATGGTGGTTCTGAGGCTGTCACAGATGATATGGAACATGACCAAGATCTTGAAGGACGTTTTGCTGCTGCTAATGAAGATGAGTATATGCATGAAATTTCAGAGGATACTCGGGGTTTGGAAAACGGCATTGATACAATGGTGGAAGTTTTTGGGTCTAGACGTCAAGGGCGCACTACTTCTATTTACAGTCTTTTGGGCAGAACAGGTGAGAATGCGGCTCCATCAAGGCATCCTCTTTTGGTTGGACCTTTGTCTCTGCATTCGGCCCCTCCTAGGCAACCAGAAAATTCTCGTGATGGCATCATACCAGATAGGAACTCTGAGATCACCTCATCAAGATTGGACAGTATTTTCCGATCATTGAGGAATGGGCGCCATGGACACCGTCTGAACTTGTGGATGGATGATAACCAGCAAGGTGGTGGATCAAATGCCGGTGTAGTACCCCAAGGACTTGAGGAGTTGCTTGTTTCCCAATTGAGGCGACCAACACCCGAAAAGACTTCTGAAGGGAATACAACACCAGTAGATTCTCAGAACAAAGAAGTAGTTGAGTTGCAAGAACCGGATGCAGGTGTAAGGCCTGAAATGCCTGTTGAAAATGATATAAATAATGATAGTGCTAATGTGCCCCCTGCTGATACAATGGTCAACTCTGGCAATGCTGATTTGGTAACACCAACAGCAAGTGAGTCTTTACAAGCAATGGACATGTCTAGCACACATCCACAGTCTGTTGAGATGCAGTTTGAACACACTGATGCAGCTGTGCGGGATGTAGAAGCTGTGAGCCAAGAAAGTGGTGGAAGTGGAGCAACTTTAGGGGAGAGCCTTCGTAGCCTTGATGTTGAAATTGGGAGTGCTGATGGTCATGATGATGGTGCAGATAGGCAAGGGTCCTCAGATAGAATGCCACTTGGTGATTCTCAGGCGACTCGAGCGAGGAGAACGAACATATCTTTTGGGAATTCTGCATCTGCAAATGCAAGAGATGTTTCACTTCATAGTGTAACTGAGGTTTCAGAAAATTCTAGCCGGGAAGCAGATCAGGATGGTCCAGCAGCAGAACAGCAACTTAATAGTGATGCTGGTTCTGGGGCAATTGATCCTGCTTTCTTGGATGCTCTTCCTGAGGAGTTGCGAGCTGAAGTCCTCTCTGCACAACAGGGTCAAGTAGCTCCACAGTCAGCCTCTGAGCCACAAAATGCTGGTGATATTGATCCAGAATTCCTTGCTGCCCTTCCCCCGGATATCAGGGCTGAAGTTTTAGCTCAACAGCAAGCCCAAAGGGTACATCAGTCTCAGGAACTGGAAGGTCAACCAGTTGAAATGGACACTGTTTCAATAATTGCAACCTTTCCTTCAGAATTGAGAGAAGAGGTTCTGTTAACTTCATCTGATGCTATACTTGCCAATCTTACACCGGCTCTAGTTGCGGAGGCAAACATGTTGCGCGAAAGATTTGCCAATCGTTATAATCGTACCCTATTTGGTATGTATCCTAGAAACCGTAGGGGTGAGGCGTCAAGACCTGGCATTGGATCCAGCCTGGACAGAATTGGTGGAGGCCTTGCCTCACGTAGGCCTATAGGTGCTAAGGTAGTTGAAGCTGAAGGAGCTCCTTTAGTCGATGCGGAAGCTCTGCATGCAATGATTCGAGTGCTTCGAATGTTTCAGCCTCTATATAAAGGTCAGCTGCAGAAGCTTCTGTTGAATTTATGTGCTCATGTTGAAAGCAGAAATTCTGTTGTGAGAATTCTGATGGGCATACTAATGCTTGATACAAGAAAGCCTGGAAATCACTCAACTGATATCGAAACATCGTATCGGCTTTATGGTTGCCAGGGCAATGTGATGTATTCACGCGCCTCAGGAGTTCCACCATTGTTATCTCGCCGCATTCTGGAAACTCTGACATATTTGGCTCGGCATCATCCTAACGTGGCAAAGATTTTGCTTCATTTAAGGCTGCCTCATTCTGCTCTTCAAGAAAGAGAGAATAGTGATAGGGCTCGTGGAAAAGCTGTGATGGTTGTGGAGGAGAATGGTCAAAATAATCAGGAAGGATATCTGTCCATAGCCTTGCTTTTGAGCCTCTTGAATCAACCCCTTTATTTGACGAGAAGTATAGCTCATCTTGAACAGCTACTTAACTTATTGGATGTCATCATTGATAATGCTGAAACCAAGTTACCGGAAAAATCTGGGGTTTCTATTACTGAACAACCCTCGGCTCCTCAAGCTTCTGATGCTGAGATGAATACAGAGTCTGGTGGCACGTCCGTTGATGTTGGAACGCAAAGTAAGGTTGTTGATTCCTCCAAACCCTCCACTTCTGGTGCAGATGACGAATGCCATACTGAAAGTGTTCTGCTGAACCTGCCACAAGCAGAACTTCGCCTTCTATGTTCATTGCTTGCACGAGAAGGGCTTTCAGATAATGCTTATACTCTTGTGGCTGAGGTAATGAAGAAGTTGGTGGCAATTGTTCCTACCCATTCCAATCTGTTTATTACCGAGCTAGCAGATGCTGTAAGAAATTTGACTAGATCTGCCATGGATGAGTTGCGCAAGTTTGGTGAAGCAGTGAAAGCGCTCCTAAGTACCAAGTCGTCTGATGGAGCTGCTATTTTAAGAGTTCTGCAGGCTTTAAGCTCCCTTGTTGCCTCACTGATTGAGAAAGAGAAGGATCCACAGATTCTTGCAGATAAGGAGCACACTACTCTTTCTCAGGTTTGGGATATCAATGCAGCTTTGGAACCTTTGTGGCTTGAGCTAAGCAGTTGCATAAGTAAGATAGAAAGCTATTCAGAAACCACCCCTGATTTGTTGACTTCATATAGAGCTTCCACATCTAAACCATCTGGTGTAATTCCTCCCCTGCCTGCTGGGACTCAAAATATTTTGCCATACATAGAATCTTTCTTTGTGGTTTGTGAGAAATTGCATCCTGGGCAGCCAGGCCCTGGTAATGACTTCAGTGTTGCTGCAGTTTCTGAGGTTGATGATGCCAGTACATCTGCTAGCCTGCAGAAAACCTCGGTTCCCACTGTGAAAGTGGATGAGAAACATGTTGCTTTTATGAAGTTCTCAGAGAAACATAGGAAGTTGCTAAATGCTTTCATCCGTCAAAATCCTGGTTTGCTAGAAAAGTCTTTCTCGCTGATGCTGAAGGTTCCTCGCTTTATTGATTTTGACAACAAACGTGCTCACTTCAGGTCAAAGATTAAGCATCAACATGATCATCATCACAATCCTCTAAGAATTTCTGTAAGAAGAGCATACATTCTTGAAGATTCATATAACCAGCTTCGCATGCGGTCAGCTCTAGATTTGAAGGGGAGATTGACTGTTCACTTTCAAGGAGAGGAAGGAATTGATGCTGGTGGACTCACGAGGGAATGGTATCAGTTGCTGTCCAGGGTTATCTTTGACAAGGGAGCACTACTGTTTACAACAGTGGGCAATGAATCAACTTTCCAACCCAACCCTAACTCTGTTTACCAGACAGAACATCTTTCATATTTCAAGTTTGTTGGGCGAGTGGTTGGGAAAGCACTTTTCGATGGTCAATTATTGGATGTCCATTTTACTCGATCGTTCTACAAGCACATTTTGGGGGCTAAGGTGACTTATCATGATATTGAGGCCATTGATCCTGATTACTTTAAAAACCTCAAGTGGATGCTCGAGAATGATATAAGTGATGTTCTAGACCTTACTTTCAGTATTGATGCAGATGAGGAGAAACTAATATTATATGAAAGAACAGAAGTAACTGACTATGAACTGATTCCTGGAGGGCAAAACATTAAAGTTACAGAAGAAAATAAGCACCAATATGTTGACTTAGTTGCGGAGCATCGATTAACAACTGCTATTCGTCCCCAGATAAATGCGTTTTTGGATGGATTCTCTGAATTAATTCCTAGGGACCTAATATCCATTTTCAATGACAAGGAACTGGAACTATTGATCAGTGGGCTTCCAGATATTGACTTGGATGACATGAGGGCAAATACCGAATATTCTGGATACAGCTCTGGATCTCCTGTGATACAGTGGTTTTGGGAGGTTGTCCAAGCTCTTAGTAAGGAGGATAAAGCTCGACTATTGCAGTTTGTGACTGGCACCTCGAAGGTACCTTTAGAAGGTTTTAGTGCACTTCAGGGAATTTCAGGCTCACAGAAGTTCCAGATACACAAAGCATACGGAAGCCCTGATCACTTGCCATCTGCACACACTTGTTTCAATCAGTTGGATTTACCAGAGTATCCTTCTAAGCAACATCTTGAAGAGAGATTGCTTCTTGCGATTCACGAAGCCAACGAGGGATTTGGTTTTGGTTAA

Protein Analysis

3249

Amino Acids

356.3

Weight (kDa)

5.4

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF908 PF06012 3 - 148 5.3e-15 Domain of Unknown Function (DUF908)
DUF913 PF06025 236 - 508 2.7e-51 Domain of Unknown Function (DUF913)
UBA PF00627 1003 - 1040 2.8e-09 UBA/TS-N domain
UBA_7 PF22562 1004 - 1050 3.5e-14 UBA-like domain
UBM PF14377 2168 - 2191 2.9e-07 Ubiquitin binding region
UBM PF14377 2202 - 2230 2.6e-11 Ubiquitin binding region
UBM PF14377 2239 - 2270 8.9e-07 Ubiquitin binding region
HECT PF00632 2938 - 3248 2.4e-99 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 6876
AarI CACCTGC 2 cut(s) 3637, 5963
AatII GACGTC 1 cut(s) 5563
Acc16I TGCGCA 1 cut(s) 7970
Acc36I ACCTGC 5 cut(s) 135, 3342, 3637, 5963, 7788
Acc65I GGTACC 2 cut(s) 70, 9541
AccB1I GGYRCC 5 cut(s) 70, 4613, 5772, 9530, 9541
AccB7I CCANNNNNTGG 6 cut(s) 1426, 2845, 4894, 5146, 5323, 8366
AccBSI CCGCTC 2 cut(s) 361, 3458
AccI GTMKAC 5 cut(s) 2308, 2485, 2892, 3278, 4469
AccII CGCG 4 cut(s) 2097, 4822, 6855, 7266
AclI AACGTT 1 cut(s) 855
AcvI CACGTG 1 cut(s) 2810
AcyI GRCGYC 3 cut(s) 5560, 5773, 6923
AdeI CACNNNGTG 1 cut(s) 6320
AfeI AGCGCT 1 cut(s) 7995
AflIII ACRYGT 4 cut(s) 5221, 6111, 6846, 8470
AhlI ACTAGT 1 cut(s) 2102
AjiI CACGTC 1 cut(s) 7680
AjuI GAANNNNNNNTTGG 6 cut(s) 919, 951, 1070, 1102, 1646, 1678
AleI CACNNNNGTG 1 cut(s) 1018
Alw44I GTGCAC 2 cut(s) 3165, 9561
Ama87I CYCGRG 3 cut(s) 5508, 6336, 9089
Aor51HI AGCGCT 1 cut(s) 7995
ApaLI GTGCAC 2 cut(s) 3165, 9561
ArsI GACNNNNNNTTYG 4 cut(s) 3349, 3381, 5208, 5240
AseI ATTAAT 1 cut(s) 9323
Asp700I GAANNNNTTC 7 cut(s) 2119, 3246, 4108, 6780, 7767, 9192, 9324
Asp718I GGTACC 2 cut(s) 70, 9541
AspA2I CCTAGG 2 cut(s) 5672, 9329
AsuC2I CCSGG 2 cut(s) 6437, 6647
AsuII TTCGAA 2 cut(s) 3776, 7066
AsuNHI GCTAGC 3 cut(s) 1282, 7921, 8423
AvaI CYCGRG 3 cut(s) 5508, 6336, 9089
AvrII CCTAGG 2 cut(s) 5672, 9329
AxyI CCTNAGG 3 cut(s) 1637, 6531, 7270
BaeGI GKGCMC 7 cut(s) 367, 2989, 3169, 3603, 4618, 6033, 9565
BamHI GGATCC 6 cut(s) 3086, 4024, 4156, 5312, 6941, 8089
BanI GGYRCC 5 cut(s) 70, 4613, 5772, 9530, 9541
BanII GRGCYC 5 cut(s) 331, 2210, 2611, 7021, 7414
BauI CACGAG 6 cut(s) 117, 3162, 5691, 7413, 7821, 8794
BbrPI CACGTG 1 cut(s) 2810
BbsI GAAGAC 5 cut(s) 717, 950, 1191, 1341, 3153
BbvCI CCTCAGC 1 cut(s) 7860
BceAI ACGGC 1 cut(s) 5539
BcgI CGANNNNNNTGC 4 cut(s) 3140, 3174, 6845, 6879
BciVI GTATCC 4 cut(s) 5497, 6909, 9428, 9684
BclI TGATCA 7 cut(s) 1393, 2380, 5190, 5278, 8641, 9375, 9622
BcnI CCSGG 2 cut(s) 6437, 6647
BcuI ACTAGT 1 cut(s) 2102
BfoI RGCGCY 2 cut(s) 5776, 7997
BfuAI ACCTGC 5 cut(s) 135, 3342, 3637, 5963, 7788
BfuI GTATCC 4 cut(s) 5497, 6909, 9428, 9684
BglII AGATCT 5 cut(s) 7, 797, 3547, 5443, 7949
BlnI CCTAGG 2 cut(s) 5672, 9329
BlpI GCTNAGC 3 cut(s) 3678, 5364, 8177
BmcAI AGTACT 1 cut(s) 1312
BmeT110I CYCGRG 3 cut(s) 5508, 6336, 9089
BmgBI CACGTC 1 cut(s) 7680
BmrI ACTGGG 1 cut(s) 5311
BmtI GCTAGC 3 cut(s) 1286, 7925, 8427
BmuI ACTGGG 1 cut(s) 5311
BoxI GACNNNNGTC 2 cut(s) 2259, 5650
BpiI GAAGAC 5 cut(s) 717, 950, 1191, 1341, 3153
BplI GAGNNNNNCTC 2 cut(s) 5714, 5746
BpmI CTGGAG 5 cut(s) 416, 2510, 3034, 5343, 9219
Bpu10I CCTNAGC 3 cut(s) 6996, 7860, 9024
Bpu1102I GCTNAGC 3 cut(s) 3678, 5364, 8177
Bpu14I TTCGAA 2 cut(s) 3776, 7066
BpuEI CTTGAG 5 cut(s) 4040, 5876, 7623, 8192, 9062
BpuMI CCSGG 2 cut(s) 6437, 6647
Bsa29I ATCGAT 1 cut(s) 9265
BsaAI YACGTR 2 cut(s) 2810, 6980
BsaBI GATNNNNATC 2 cut(s) 6618, 8646
BsaHI GRCGYC 3 cut(s) 5560, 5773, 6923
BsaI GGTCTC 4 cut(s) 1040, 2488, 2813, 3324
BsaWI WCCGGW 2 cut(s) 5965, 7594
Bse118I RCCGGY 2 cut(s) 5834, 6823
Bse21I CCTNAGG 3 cut(s) 1637, 6531, 7270
Bse8I GATNNNNATC 2 cut(s) 6618, 8646
BseCI ATCGAT 1 cut(s) 9265
BseJI GATNNNNATC 2 cut(s) 6618, 8646
BseRI GAGGAG 7 cut(s) 3281, 5873, 6358, 6548, 7454, 7626, 9158
BseSI GKGCMC 7 cut(s) 367, 2989, 3169, 3603, 4618, 6033, 9565
BseYI CCCAGC 5 cut(s) 989, 1788, 2278, 3929, 8288
BsgI GTGCAG 5 cut(s) 1593, 6303, 6543, 7761, 9621
Bsh1236I CGCG 4 cut(s) 2097, 4822, 6855, 7266
Bsh1285I CGRYCG 3 cut(s) 275, 2095, 8999
BshNI GGYRCC 5 cut(s) 70, 4613, 5772, 9530, 9541
BshVI ATCGAT 1 cut(s) 9265
BsiEI CGRYCG 3 cut(s) 275, 2095, 8999
BsiHKCI CYCGRG 3 cut(s) 5508, 6336, 9089
BsiSI CCGG 7 cut(s) 3233, 5835, 5966, 6436, 6647, 6824, 7595
BslFI GGGAC 6 cut(s) 2985, 3578, 5305, 8305, 9270, 9347
BsmFI GGGAC 6 cut(s) 2985, 3578, 5305, 8305, 9270, 9347
BsmI GAATGC 7 cut(s) 1459, 1966, 5617, 5659, 6315, 7299, 7757
Bso31I GGTCTC 4 cut(s) 1040, 2488, 2813, 3324
BsoBI CYCGRG 3 cut(s) 5508, 6336, 9089
Bsp119I TTCGAA 2 cut(s) 3776, 7066
Bsp1407I TGTACA 2 cut(s) 150, 1531
Bsp1720I GCTNAGC 3 cut(s) 3678, 5364, 8177
Bsp19I CCATGG 6 cut(s) 1572, 2622, 2844, 5322, 5776, 7956
BspDI ATCGAT 1 cut(s) 9265
BspFNI CGCG 4 cut(s) 2097, 4822, 6855, 7266
BspHI TCATGA 5 cut(s) 355, 811, 4381, 6271, 9037
BspMAI CTGCAG 8 cut(s) 146, 1378, 3300, 3876, 7101, 8046, 8392, 8433
BspMI ACCTGC 5 cut(s) 135, 3342, 3637, 5963, 7788
BspOI GCTAGC 3 cut(s) 1286, 7925, 8427
BspQI GCTCTTC 8 cut(s) 1173, 1636, 1890, 1971, 2597, 6531, 7389, 8671
BspT104I TTCGAA 2 cut(s) 3776, 7066
BspT107I GGYRCC 5 cut(s) 70, 4613, 5772, 9530, 9541
BspTNI GGTCTC 4 cut(s) 1040, 2488, 2813, 3324
BsrBI CCGCTC 2 cut(s) 361, 3458
BsrFI RCCGGY 2 cut(s) 5834, 6823
BsrGI TGTACA 2 cut(s) 150, 1531
BssAI RCCGGY 2 cut(s) 5834, 6823
BssNAI GTATAC 1 cut(s) 4470
BssNI GRCGYC 3 cut(s) 5560, 5773, 6923
BssSI CACGAG 6 cut(s) 117, 3162, 5691, 7413, 7821, 8794
Bst1107I GTATAC 1 cut(s) 4470
Bst2BI CACGAG 6 cut(s) 117, 3162, 5691, 7413, 7821, 8794
BstACI GRCGYC 3 cut(s) 5560, 5773, 6923
BstAPI GCANNNNNTGC 5 cut(s) 162, 317, 2372, 4637, 4688
BstAUI TGTACA 2 cut(s) 150, 1531
BstBAI YACGTR 2 cut(s) 2810, 6980
BstBI TTCGAA 2 cut(s) 3776, 7066
BstDSI CCRYGG 7 cut(s) 1572, 2349, 2622, 2844, 5322, 5776, 7956
BstEII GGTNACC 1 cut(s) 5209
BstENI CCTNNNNNAGG 4 cut(s) 1353, 4040, 6979, 9549
BstFNI CGCG 4 cut(s) 2097, 4822, 6855, 7266
BstH2I RGCGCY 2 cut(s) 5776, 7997
BstMCI CGRYCG 3 cut(s) 275, 2095, 8999
BstPAI GACNNNNGTC 2 cut(s) 2259, 5650
BstPI GGTNACC 1 cut(s) 5209
BstSLI GKGCMC 7 cut(s) 367, 2989, 3169, 3603, 4618, 6033, 9565
BstUI CGCG 4 cut(s) 2097, 4822, 6855, 7266
BstV2I GAAGAC 5 cut(s) 717, 950, 1191, 1341, 3153
BstXI CCANNNNNNTGG 6 cut(s) 1066, 3097, 3181, 5399, 5819, 6200
BstZ17I GTATAC 1 cut(s) 4470
Bsu15I ATCGAT 1 cut(s) 9265
Bsu36I CCTNAGG 3 cut(s) 1637, 6531, 7270
BsuI GTATCC 4 cut(s) 5497, 6909, 9428, 9684
BsuTUI ATCGAT 1 cut(s) 9265
BtgI CCRYGG 7 cut(s) 1572, 2349, 2622, 2844, 5322, 5776, 7956
BtgZI GCGATG 1 cut(s) 4397
BtrI CACGTC 1 cut(s) 7680
BtsI GCAGTG 2 cut(s) 2865, 7992
BveI ACCTGC 5 cut(s) 135, 3342, 3637, 5963, 7788
CciI TCATGA 5 cut(s) 355, 811, 4381, 6271, 9037
Cfr10I RCCGGY 2 cut(s) 5834, 6823
ClaI ATCGAT 1 cut(s) 9265
CseI GACGC 3 cut(s) 4730, 5076, 6912
CsiI ACCWGGT 1 cut(s) 2724
CspCI CAANNNNNGTGG 4 cut(s) 1155, 1190, 2331, 2366
DinI GGCGCC 1 cut(s) 5774
DraI TTTAAA 3 cut(s) 36, 688, 9070
DraIII CACNNNGTG 1 cut(s) 6320
Ecl136II GAGCTC 3 cut(s) 329, 2208, 7019
Eco147I AGGCCT 5 cut(s) 3376, 4219, 5983, 6969, 6985
Eco24I GRGCYC 5 cut(s) 331, 2210, 2611, 7021, 7414
Eco31I GGTCTC 4 cut(s) 1040, 2488, 2813, 3324
Eco32I GATATC 4 cut(s) 6652, 7213, 7469, 8143
Eco47III AGCGCT 1 cut(s) 7995
Eco53kI GAGCTC 3 cut(s) 329, 2208, 7019
Eco72I CACGTG 1 cut(s) 2810
Eco81I CCTNAGG 3 cut(s) 1637, 6531, 7270
Eco88I CYCGRG 3 cut(s) 5508, 6336, 9089
Eco91I GGTNACC 1 cut(s) 5209
EcoICRI GAGCTC 3 cut(s) 329, 2208, 7019
EcoNI CCTNNNNNAGG 4 cut(s) 1353, 4040, 6979, 9549
EcoO109I RGGNCCY 4 cut(s) 2833, 6297, 8363, 9334
EcoO65I GGTNACC 1 cut(s) 5209
EcoRI GAATTC 5 cut(s) 3424, 3664, 6367, 6626, 7155
EcoRV GATATC 4 cut(s) 6652, 7213, 7469, 8143
EcoT22I ATGCAT 2 cut(s) 3864, 5490
EcoT38I GRGCYC 5 cut(s) 331, 2210, 2611, 7021, 7414
EgeI GGCGCC 1 cut(s) 5774
EheI GGCGCC 1 cut(s) 5774
FaqI GGGAC 6 cut(s) 2985, 3578, 5305, 8305, 9270, 9347
FauI CCCGC 4 cut(s) 354, 4124, 5282, 6165
FauNDI CATATG 1 cut(s) 775
FbaI TGATCA 7 cut(s) 1393, 2380, 5190, 5278, 8641, 9375, 9622
FblI GTMKAC 5 cut(s) 2308, 2485, 2892, 3278, 4469
FriOI GRGCYC 5 cut(s) 331, 2210, 2611, 7021, 7414
FspI TGCGCA 1 cut(s) 7970
GsaI CCCAGC 5 cut(s) 993, 1792, 2282, 3933, 8292
GsuI CTGGAG 5 cut(s) 416, 2510, 3034, 5343, 9219
HaeII RGCGCY 2 cut(s) 5776, 7997
HapII CCGG 7 cut(s) 3233, 5835, 5966, 6436, 6647, 6824, 7595
HgaI GACGC 3 cut(s) 4730, 5076, 6912
Hin1I GRCGYC 3 cut(s) 5560, 5773, 6923
HincII GTYRAC 8 cut(s) 1108, 2309, 2893, 6052, 6719, 6789, 8232, 9247
HindII GTYRAC 8 cut(s) 1108, 2309, 2893, 6052, 6719, 6789, 8232, 9247
HpaI GTTAAC 1 cut(s) 6789
HpaII CCGG 7 cut(s) 3233, 5835, 5966, 6436, 6647, 6824, 7595
Hpy99I CGWCG 2 cut(s) 2310, 2313
HpyCH4IV ACGT 8 cut(s) 855, 2809, 5455, 5560, 6979, 7341, 7679, 8605
HpySE526I ACGT 8 cut(s) 855, 2809, 5455, 5560, 6979, 7341, 7679, 8605
Hsp92I GRCGYC 3 cut(s) 5560, 5773, 6923
KasI GGCGCC 1 cut(s) 5772
KpnI GGTACC 2 cut(s) 74, 9545
Ksp22I TGATCA 7 cut(s) 1393, 2380, 5190, 5278, 8641, 9375, 9622
KspAI GTTAAC 1 cut(s) 6789
LguI GCTCTTC 8 cut(s) 1173, 1636, 1890, 1971, 2597, 6531, 7389, 8671
MabI ACCWGGT 1 cut(s) 2724
MaeII ACGT 8 cut(s) 855, 2809, 5455, 5560, 6979, 7341, 7679, 8605
MbiI CCGCTC 2 cut(s) 361, 3458
MfeI CAATTG 5 cut(s) 2524, 2585, 5876, 6501, 7884
Mly113I GGCGCC 1 cut(s) 5773
Mph1103I ATGCAT 2 cut(s) 3864, 5490
MroXI GAANNNNTTC 7 cut(s) 2119, 3246, 4108, 6780, 7767, 9192, 9324
MspA1I CMGCKG 7 cut(s) 674, 1788, 2336, 3877, 5363, 6167, 7096
MspI CCGG 7 cut(s) 3233, 5835, 5966, 6436, 6647, 6824, 7595
MunI CAATTG 5 cut(s) 2524, 2585, 5876, 6501, 7884
Mva1269I GAATGC 7 cut(s) 1459, 1966, 5617, 5659, 6315, 7299, 7757
MvnI CGCG 4 cut(s) 2097, 4822, 6855, 7266
NarI GGCGCC 1 cut(s) 5773
NciI CCSGG 2 cut(s) 6437, 6647
NcoI CCATGG 6 cut(s) 1572, 2622, 2844, 5322, 5776, 7956
NdeI CATATG 1 cut(s) 775
NheI GCTAGC 3 cut(s) 1282, 7921, 8423
NmeAIII GCCGAG 2 cut(s) 7306, 7610
NmuCI GTSAC 7 cut(s) 872, 892, 2228, 4244, 5416, 9028, 9523
NsbI TGCGCA 1 cut(s) 7970
NsiI ATGCAT 2 cut(s) 3864, 5490
NspV TTCGAA 2 cut(s) 3776, 7066
OliI CACNNNNGTG 1 cut(s) 1018
PaeI GCATGC 4 cut(s) 2405, 3866, 7049, 8719
PaeR7I CTCGAG 2 cut(s) 6336, 9089
PagI TCATGA 5 cut(s) 355, 811, 4381, 6271, 9037
PaqCI CACCTGC 2 cut(s) 3637, 5963
PceI AGGCCT 5 cut(s) 3376, 4219, 5983, 6969, 6985
PciI ACATGT 4 cut(s) 5221, 6111, 6846, 8470
PciSI GCTCTTC 8 cut(s) 1173, 1636, 1890, 1971, 2597, 6531, 7389, 8671
PcsI WCGNNNNNNNCGW 4 cut(s) 124, 6877, 7063, 9726
PctI GAATGC 7 cut(s) 1459, 1966, 5617, 5659, 6315, 7299, 7757
PdmI GAANNNNTTC 7 cut(s) 2119, 3246, 4108, 6780, 7767, 9192, 9324
PflFI GACNNNGTC 1 cut(s) 5784
PflMI CCANNNNNTGG 6 cut(s) 1426, 2845, 4894, 5146, 5323, 8366
PfoI TCCNGGA 1 cut(s) 9196
Ple19I CGATCG 1 cut(s) 8999
PluTI GGCGCC 1 cut(s) 5776
PmaCI CACGTG 1 cut(s) 2810
PmlI CACGTG 1 cut(s) 2810
Ppu21I YACGTR 2 cut(s) 2810, 6980
PpuMI RGGWCCY 3 cut(s) 2833, 6297, 9334
PscI ACATGT 4 cut(s) 5221, 6111, 6846, 8470
PshAI GACNNNNGTC 2 cut(s) 2259, 5650
PshBI ATTAAT 1 cut(s) 9323
PsiI TTATAA 1 cut(s) 6876
Psp124BI GAGCTC 3 cut(s) 331, 2210, 7021
Psp1406I AACGTT 1 cut(s) 855
Psp5II RGGWCCY 3 cut(s) 2833, 6297, 9334
PspCI CACGTG 1 cut(s) 2810
PspEI GGTNACC 1 cut(s) 5209
PspFI CCCAGC 5 cut(s) 989, 1788, 2278, 3929, 8288
PspPPI RGGWCCY 3 cut(s) 2833, 6297, 9334
PspXI VCTCGAGB 1 cut(s) 6336
PsrI GAACNNNNNNTAC 2 cut(s) 978, 1010
PstI CTGCAG 8 cut(s) 146, 1378, 3300, 3876, 7101, 8046, 8392, 8433
PsyI GACNNNGTC 1 cut(s) 5784
PvuI CGATCG 1 cut(s) 8999
PvuII CAGCTG 5 cut(s) 674, 3877, 5363, 6167, 7096
SacI GAGCTC 3 cut(s) 331, 2210, 7021
SalI GTCGAC 2 cut(s) 2307, 2891
SapI GCTCTTC 8 cut(s) 1173, 1636, 1890, 1971, 2597, 6531, 7389, 8671
ScaI AGTACT 1 cut(s) 1312
SexAI ACCWGGT 1 cut(s) 2724
SfoI GGCGCC 1 cut(s) 5774
Sfr274I CTCGAG 2 cut(s) 6336, 9089
SfuI TTCGAA 2 cut(s) 3776, 7066
SgrDI CGTCGACG 1 cut(s) 2307
SlaI CTCGAG 2 cut(s) 6336, 9089
SmlI CTYRAG 7 cut(s) 4019, 5855, 6336, 7638, 8171, 9077, 9089
SmoI CTYRAG 7 cut(s) 4019, 5855, 6336, 7638, 8171, 9077, 9089
SpeI ACTAGT 1 cut(s) 2102
SphI GCATGC 4 cut(s) 2405, 3866, 7049, 8719
SseBI AGGCCT 5 cut(s) 3376, 4219, 5983, 6969, 6985
SspDI GGCGCC 1 cut(s) 5772
SspI AATATT 6 cut(s) 550, 3991, 4785, 8302, 9156, 9428
SstI GAGCTC 3 cut(s) 331, 2210, 7021
StuI AGGCCT 5 cut(s) 3376, 4219, 5983, 6969, 6985
TaiI ACGT 8 cut(s) 858, 2812, 5458, 5563, 6982, 7344, 7682, 8608
TaqII GACCGA 1 cut(s) 2081
TatI WGTACW 6 cut(s) 150, 427, 720, 1310, 1531, 8414
TauI GCSGC 4 cut(s) 736, 1679, 5618, 7299
TseFI GTSAC 7 cut(s) 872, 892, 2228, 4244, 5416, 9028, 9523
Tsp45I GTSAC 7 cut(s) 872, 892, 2228, 4244, 5416, 9028, 9523
TspGWI ACGGA 8 cut(s) 116, 2121, 2338, 2682, 3998, 7672, 8516, 9627
Tth111I GACNNNGTC 1 cut(s) 5784
Van91I CCANNNNNTGG 6 cut(s) 1426, 2845, 4894, 5146, 5323, 8366
VneI GTGCAC 2 cut(s) 3165, 9561
VspI ATTAAT 1 cut(s) 9323
XagI CCTNNNNNAGG 4 cut(s) 1353, 4040, 6979, 9549
XbaI TCTAGA 6 cut(s) 1888, 4456, 5200, 5555, 8728, 9112
XcmI CCANNNNNNNNNTGG 5 cut(s) 1906, 5399, 5819, 6200, 8455
XhoI CTCGAG 2 cut(s) 6336, 9089
XmaJI CCTAGG 2 cut(s) 5672, 9329
XmiI GTMKAC 5 cut(s) 2308, 2485, 2892, 3278, 4469
XmnI GAANNNNTTC 7 cut(s) 2119, 3246, 4108, 6780, 7767, 9192, 9324
ZraI GACGTC 1 cut(s) 5561
ZrmI AGTACT 1 cut(s) 1312
Zsp2I ATGCAT 2 cut(s) 3864, 5490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.