Rh7CG462100

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0012629
Physical Location & Seq
Forward (+)
61704131 .. 61719541
15411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG462100.1

Sequence Viewer

Length: 10506 bp
ATGACTACTGTAAGATCAAGCTTGCCGTCCAGGATTCGGCAGCTTCTGTCCGCCGACGGTGCTCTCGGGCCGTCTATCAAACTTGACTCCGAGCCGTCTCCTAAGATCAAAGCCTTTATCAATAAGGTGATACAGTGTCCATTACAAGATATAGCTATTCCTCTTTCCGGTTTCCGCTGGGAGTACAGTAAGGGTAATTTCAACCATTGGAGGCCGCTTTTTCTGCATTTCGATACATATTTCAAGACATACTTGTCTGGTAGAAATGACCTACTCTTGTCCGATAAAATCTTAGAAGATGATACTCCATTCCCAAAACATGCAGTACTACAAATTTTGAGAGTGATGCAAACAATTTTAGAGAATTGCCATAACAAGAGTTCTTTTGATGGCTTAGAGCATTTCAAGCTGCTACTTGCATCAACAGATCCAGAAGTTCTTATAGCAACACTTGAAACACTTTCTGCGCTTGTGAAAATATATCCCTCTAAGATGCATGTCAGTGGAAAGATGATCGGCTGTGGTTCTGTAAACAGCTATCTCCTGTCGCTTGCACAGGGCTGGGGAAGCAAGGAAGAAGGGTTGGGTCTATTTTCATGTGTCATGGCAAACGAGACTGCCCAAGATGACGGGCTGAATTTGTTCCCATCTGATGTAGAAAATGATTCTGACAAATCTCAGCATCGAATAGGTTCTACCCTTTATTTTGAATTGCATGGGAATGCCCAAAGTACAGAGGAGAGCAGTAGCAATGTAAATAGTACTTCCAGTTTGGGGGTTATAAACATGCCAGATCTGCATTTGCAGAAGGAGGATGATTTAAAGCTGATGAAACAGTGCATAGAGGACTACAGGGTACCTCCTGAGATTCGGTTCTCACTCCTGACAAGAATCAGATATGCTCGTGCCTTCCGTTCACCAAGAATCTGCAGGTTGTACAGCAGGATTTGCCTTCTTGCATTTATTGTGCTTGTTCAATCTAGTGATGCACATGAAGAACTTGTATCATTCTTTGCTAATGAGCCAGAATATACTAATGAACTGATTAGAATTGTGCGGTCGGAAGAATCAGTTTCTGGAACCATCAGAACACATGCAATGCTTGCCTTGGGAGCTCAATTAGCTGCTTATTCTGCATCTCATGAGCGGGCACGAATTTTGAGTGGATCTAGTATCAGTTTTGCAGGAGGGAATCGTATGATACTTCTGAATGTACTCCAGAGAGCAGTTTTATCACTTAAAACCTCCAATGATCCAACTTCTCTTGCTTTTGTTGAAGCTCTGCTTCAGTTCTATTTGCTCCATGTGGTTTCATCATCCACTACAGGGAGTAATATTAGAGGGTCAGGCATGGTTCCAACATTCTTGCCTCTTTTGGAGGATTCTGATCCCTTGCACTTGCATCTAGTCTGTTTTGCTGTGAAAACTCTGCAAAAGCTCATGGATTACAGTAGTTCAGCTGTGTCTCTCTTTAAAGAGTTAGGGGGAGTTGAACTGTTGGCTCAGAGACTACAGATAGAGGTACATAGGGTGATAGGCTTGGTCGGCGATAATGATAATTCAATGGTCATTGGTGAAAGCTCCAGATATAATGAAGACCAGTTGTACTCCCAGAAGAGGCTCATTAAAGCTTCGTTGAAGGCACTTGGGTCTGCTTCATATGCTGCTGGCAATTCTACCAGATCTCAGCATCCTCATGACAGTTCTTTACCTGAAACTCTTTCTCTGATCTTTGGGAACGTTGATAAGTTTGGAGGTGACATTTATCACTCTGCTGTGACAGTTCTGAGTGAAACGATACACAAAGACCCAACTTGTTATTCTGCTTTGCACGATATGGGTCTTCCAGATGCTTTCATATCTTCAGTTGTAGCTGGGGTTCTTCCATCTGCAAAGGCACTTCAGTGTGTGCCTAATGGTCTCGGTGCTATATGTCTTAACGCCAAAGGCTTGGAGGCTGTGAAGGAAAAGTTGGCCTTACGATTTCTTGTTGACATCTTCACCAGCAAGAAGTATGTAGTAGCAATGAATGGAGCTATTGTCCATTTGACAAATGCTGTGGAAGAGCTTTTGCGCCATGTATCTTCGCTAAGAAGTATTGGTGTTGATATTATGATTGAAATTGTTGAAAAAATTGCTTCCTTCACTGACAACAACAATGCAGGGCTAGCAGGAAAGGCTAGTGGGAGTGGGAGTACTGCTATGGAAATGGATTCTGAAGACAAGGAACAGCCTTTGGTAGGTGCTGTAGAATCGGCTGCAGATGGTATCAGTAATGATCAGTTCATTCAATTATCCATTTTCCATTTGATGGTACTGGTCCACAGAACAATGGAGAATGCTGAAATATGTCGGTTATTTGTTGAAAAGTCTGGAATTGATGCTTTACTGAAGCTTTTGTTACAGCCAACTGTTGTACAATCATCTGATGGAATGTCTATTGCTCTACATAGCACCATGGTTTTCAAAGATTTCACTCAAAATCACTCTGCTGCACTTGCCCGTGCATTTTGCTCTTCCCTTAGGGGCCACCTAAAGAAAGCTTTGTCGGAATTTGGTGCGGCTTCAGGGTCTTTCTTACTGGAACCTAAGATGACTTCAGATGGTGGGATCTTTCCTTCACTGTTTCTCGTTGAGTTCCTGTTGTTTATTGCTGCCTCAAAGGAAAACCGCTGGGTAACTGCATTGCTTACAGAACTTGGAAATGATAGCAAGGATGTTCTGGAAGATATTGGGCGTGTCCATCGGGAAGTTCTATGGCAGATTGCTCTTCTAGAAGATACCAAGTCTGAAATGGAGGATGAAAGTGCTGGTTCTACCACTGAGTCGCAACAGTCAGAAATGAATGCAAGTGATTCTGAAGAGCAAAGGTTCAACTCTTTTAGGCAGTTTCTTGATCCATTGATGAGAAGAAGGCAATCTGGATGGAGCATTGAATCGCAATTTCTTGATCTTATTAACCTGTATCGTGATCTCGGTCGCGCCACTAGTTCTCAAAGAACACATTCTGATGGTCCGTCAAATCTGCGGATTGGATCTAGTCATCATTCTGGTTCTTCAGATGCTGGTAGAAGGGAGTATGACCAGCAGAGCTCCTACTATACTTCTTGCTGTGACATGGTAAGATCACTTTCTTTTGACATAAGTCATTTGTTTCAAGAGCTGGGAAAGGTCATGTACCAACCTTCTCGTCGACGGGATGATATTGTGAATGTTTCCGCTGCTGCAAAATCCGTGGCTTCTACATTTGCATCTATTGCATTGGATCACATGAGTTTTGGGGGGCATGCAAGTTCTTCTGGATCTGAGGTGTCTATATCAGCAAAATGTCGTTACTTTGGTAAAGTTATTGATTTCATTGATGTCAGTCTACTGGAGAGACCAGATTCATGTAACTCTGTTTTCCTCAATTGCTTGTATGGACATGGAGTTATCCAGTCAGTTTTAACGACATTTGAAGCTACCAGTCAATTGCTCTTCACAGTAAATAGGGCTCCTGCATCTCCCATGGAAACTGATGATGGAAACTCAAAGCAGGATGAAAGGGAAGACACTGACCATTCATGGATTTATGGTCCGTTAGCTAGTTATGGAAAACTTATGGACCACCTGGTAACATCGTCTTTCATATTATCTCCTTTTACAAAACAGTTGCTTGCACAGCCCCTTGCAAATGGAACAATTCCTTTTCCACGCGATGCTGAGACCTTTGTAAAGGTCCTACAATCCATGGTGCTGAAGGCAGTGCTTCCTGTCTGGACTCATCCCCAATTTGTCGACTGTAGTTATGATTTTATTACTACAGTTATTTCTATAATCAGGCACATATACTCTGGGGTTGAAGTAAAGAATGTCAGTAGCAGTTCCAGTGCTCGTATTGCTGGTTCCCCTCCTAATGAGACAACTATATCAACGATTGTAGAGATGGGCTTCTCCAGGTCTAGGGCAGAAGAAGCTTTGAGACAGGTTGGATCCAATAGTGTGGAGTTGGCTATGGAGTGGTTGTGCTCCCATCAAGAGGAAGTTCAAGAAGACGATGAACTTGCTCGTGCACTTGCCATGTCTTTGGGTAATTCAGAGTCTGACACTAAGGAAGCTGGTGCAATGGAAAGTGCGCCGGAAGAAGAAAAGGTTCAACTTCCTCCTGTTGAGGAGTTGTTGTCTACATGCACAAAGCTTCTGCAGATGAAGGAACCTCTAGCTTTTCCAGTGCGAGACCTGCTTGTGATGATATGCTCCCAAAATGATGGTCAATGTAGGCCTAATATCATATCATTCATTGTTGATCGCGTCAAAGAATCTAGTTTGAATTCTGACAGTGGAAACAGTAACTTGCTATCTGCTCTTTTTCATGTTCTTGCTTTAATTCTTCATGAAGATGCAGTTGCAAGAGAAATAGCCTCGAAGAATGGATTTGTGAAAATAGCTTCAGATCTACTTTCGCAATGGGACTCTAGTTCAACTGGCATAGCAAAACGAGAAGTGCCCAAGTGGGTAACTACAGCTTTTCTTGCAATAGATCGGCTGTTGCAGGTGGATCAAAAATTGAATTCTGAAATTGCCGAGCAGTTGAAGAGAGATGCTGTTAGTAGCCAGCAAACATCTATTAGCATTGATGAGGACAAGCAAAATAAGTTGCAGTCTGCATTGGGATTATCTTCGAAGCACATAGAAATTAAGGAGCAGAAGAGGCTTATTGAGATTGCTTGTTCATGTATCAGGAATCAGCTTCCTTCTGACACAATGCATGCAGTTCTGCAGCTGTGCTCTACCCTCACCAAAACACATACAGTTGCTGTTAGTTTTCTTGATGCTGGGGGTTTAAGTTTGCTACTCTCTTTGCCAACAAGTAGTCTATTTTCTGGTTTTGACAATATTGCTGCAACCATTATCCGTCATGTTCTTGAGGATCCTCAAACCCTTCAGCAGGCAATGGAATTTGAAATAAGGCACAATCTTGTGGCTGCTGCCAACAGACATTCAAATGGAAGGGTTTCTCCCCGCAATTTTCTAGCGAGTTTAAGTTCTGCCATTTCCAGGGATCCAGTGATATTCATGCGTGCTGCTCAGTCTATTTGTCAAGTGGAGATGGTTGGTGAGAGGCCTTGCATTGTTTTGTTGAAAGATCGTGACAAAGATAAAACCAAAGAGAAAGAAAAAGAGAAGGATAAATCTTTGGACAAGGAGAAAATACATATTTCGGATGGGAAAACTGCTCTGGGTAATACAAATCCAGTACCTTCTGGCAATGCACAAGGCAAGGCTCATGATTCAAACTCAAAGAGTGGTAAAGTTCATCGCAAATATCCCCAGAGTTTTGTGAGTGTGATTGAGCTTCTTCTAGATTCTGTGTATACTTATGTTCCTCCTTCAAAAGACAATCTTGTTGTTGATGTGCTCCATGATACCCCGTCATCGACTGACATGGACATTGATGTTGCTGCTGTCAAAGGAAAAGGAAAAGCTATTGCCTCATCTTCTGAAGATGGTGGAGCTGGTGCCCAAGAAGCTTCTGCATCACTTGCAAAGGTCGTTTTTATTTTGAAGCTTCTGACAGAGATTCTAGCAATGTATGCTTCATCTGCCCATGTTCTACTTCGGAAAGACGCTGAAATTAGTAGTTGCAGGAACCCTAATCCAAAGGGTTCTACTGCTGTATGCACTGGTGGAATATTTCACCATGTTCTTCACAAGTTTATTCCATATTCGCGGAGTGCCAAGAAGGAAAAGAAAACAGATGGTGATTGGAAGCACAAGTTAGCAAGCAGGGCGAGCCAGTTTTTGGTTGCATCATGTGTTCGTTCTTCTGAGGCAAGGAAGAGGATTTTTACAGAGATCAGTTATATTTTCAATGACTTTGTGGACTCATGTAATGGCTTTCGACCACCTGAAAATGACATACAAGCTTTTGCTGATCTGCTTAATGATGTTCTGGCTGCTCGGACTCCTACTGGTTCATACATCTCCGCAGAAGCGTCTGCTACTTTTATAGATGTTGGTCTGGTTGGATCATTGACTCGAACCCTCCAAGTGCTGGACCTGGACCATGCTGACTCACCTAAAGTTGTTACAGGTCTGATCAAAGCTCTAGAACTGGTAACCAAAGAACATGTCCATTTGGCTGATTCAACTGCTGGTAAGGGCGACAGTTCTACAAAACCTCCTGATCAGTCTGGCAGGACAGATAATGTTGACGGTGGATCCCAGTCCATGGAGACACCATCCCAATCCCATCACAATTCTGCTCCAGCTGAGCGCACTGAGTCTTTTAATGCTGTCCAATCTTATGGTGGTTCTGAGGCTGTCACAGATGATATGGAACATGACCAAGATCTTGAAGGACGTTTTGCTGCTGCTAATGAAGACGAGTATATGCATGAAATTTCAGAGGATACTCGGGTTCTGGAGAACGGCATTGATACAATGGGTATACGGTTTGAAATTCAGCCTCATGAGCAAGAAAATCTTGAGGATGATGATGAGGATGATGAAGATATGTCTGAGGATGATGGAGATGAAGTGGATGATGATGAAGATGAAGATGATGAGGAGCATAATGATCTGGAAGATGAAGTCCATCACTTGCCACATCCTGACACTGATCAAGATGATCATGAGATTGATGACGAAGAGTTTGATGAAGAAGTATTAGAAGAAGATGATGAGGATGAGGAGGATGAGGAGGATGGAGTTATACTGAGGCTGGAGGAAGGCATAAATGGGATTAATGTTTTTGACCATATTGAGGTTTTTGGAAGAGAGCATGGTTTCCCTAATGAAACACTTCATGTGATGCCAGTGGAAGTTTTTGGGTCTAGACGTCAAGGGCGCACTACTTCTATTTACAGTCTTTTGGGCAGAACAGGTGAGAATGCGGCTCCATCAAGGCATCCTCTTTTGGTTGGACCTTTGTCTCTGCATTCGGCCCCTCCTAGGCAACCAGAAAATTCTCGTGATGGCATCATACCAGATAGGAACTCTGAGATCACCTCATCAAGATTGGACAGTATTTTCCGATCATTGAGGAATGGGCGCCATGGACACCGTCTGAACTTGTGGATGGATGATAACCAGCAAGGTGGTGGATCAAATGCCGGTGTAGTACCCCAAGGACTTGAGGAGTTGCTTGTTTCCCAATTGAGGCGACCAACACCCGAAAAGACTTCTGAAGAGAATACGACACCAGTAGATTCTCAGAACAAAGAAGTAGTTGAGTTGCAAGAACCGGATGCAGCTGTAAGGCCTGAAATGCCTGTTGAAAATGATATAAATAATGATAGTGCTAATGTGCCCCCTGCTGATACAATGGTCAACTCTGGCAATGCTGATTTGGTAACACCAACAGCAAGTGAATCTTTACAAGCAATGGACATGTCTAGCACACATCCACAGTCTGTTGAGATGCAGTTTGAACACACTGACGCAGCTGTGCGGGATGTAGAAGCTGTGAGCCAAGAAAGTGGTGGAAGTGGAGCAACTTTAGGGGAGAGCCTTCGTAGCCTTGATGTTGAAATTGGGAGTGCTGATGGTCATGATGATGGTGCAGATAGGCAAGGATCCTCAGATAGAATGCCACTTGGTGATTCTCAGGCGACTCGAGCGAGGAGAACGAACATATCTTTTGGGAATTCTGCATCTGCAAATGCAAGAGATGTTTCACTTCATAGTGTAACTGAGGTTTCAGAAAATTCTAGCCGGGAAGCAGATCAGGATGGTCCAGCAGCAGAACAGCAACTTAATAGTGATGCTGGTTCTGGGGCAATTGATCCTGCTTTCTTGGATGCTCTTCCTGAGGAGTTGCGAGCTGAAGTCCTCTCTGCACAACAGGGTCAAGTAGCTCCACAGTCAGCCTCTGAGCCACAAAATGCTGGTGATATTGATCCAGAATTCCTTGCTGCCCTTCCCCCAGATATCAGGGCTGAAGTTTTAGCTCAACAGCAAGCCCAAAGGGTACATCAGTCTCAGGAACTGGAAGGTCAACCAGTTGAAATGGACACTGTTTCAATAATTGCAACCTTTCCTTCAGAATTGAGAGAAGAGGTTCTGTTAACTTCATCTGATGCTATACTTGCCAATCTTACACCGGCTCTAGTTGCGGAGGCAAACATGTTGCGCGAAAGATTTGCCAATCGTTATAATCGTACCCTATTTGGTATGTATCCTAGAAACCGTAGGGGTGAGGCGTCAAGACCTGGCATTGGATCCAGCCTGGACAGAATCGGTGGAGGCCTTGCCTCACGTAGGCCTATAGGTGCTAAGGTAGTTGAAGCTGAAGGAGCTCCTTTAGTCGATGCGGAAGCTCTGCATGCAATGATTCGAGTGCTTCGAATGTTTCAGCCTCTATATAAAGGTCAGCTGCAGAAGCTTCTGTTGAATTTATGTGCTCATGTTGAAAGCAGAAATTCTGTTGTGAGAATTCTGATGGGCATACTAATGCTTGATACAAGAAAGCCTGGGAATCACTCAACTGATATCGAAACATCGTATCGGCTTTATGGTTGCCAGGGCAATGTGATGTATTCACGCGCCTCAGGTAAAGATGGAGTTCCACCATTGTTATCTCGCCGCATTCTGGAAACTCTGACATATTTGGCTCGGCATCATCCTAACGTGGCAAAGATTTTGCTTCATTTAAGGTTGCCTCATTCTGCTCTTCAAGAAAGAGAGAATAGTGATAGGGCTCGTGGAAAAGCTGTGATGGTTGTGGAGGAGAATGGTCAAAATAATCAGGAAGGATATCTGTCCATAGCCTTGCTTTTGAGCCTCTTGAATCAACCCCTTTATTTGACGAGAAGTATAGCTCATCTTGAACAGCTACTTAACTTATTGGATGTCATCATTGATAATGCTGAAACCAAGTTACCGGAAAAATCTGGGGTTTCTATTACTGAACAACCCTCGGCTCCTCAAGCTTCTGATGCTGAGATGAATACAGAGTCTGGTGGCATGTCCGTTGATGTTGGAACGCAAAGTAAGGTTGTTGATTCCTCCAAACCCTCCACTTCTGGTGCAGATGACGAATGCCATACTGAAAGTGTTCTGCTGAACCTGCCACAAGCAGAACTTCGCCTTCTATGTTCATTGCTTGCACGAGAAGGGCTTTCAGATAATGCTTATACTCTTGTGGCTGAGGTAATGAAGAAGTTGGTGGCAATTGTTCCTACCCATTCCAATCTGTTTATTACCGAGCTAGCAGATGCTGTTAGAAATTTGACTAGATCTGCCATGGATGAGTTGCGCAAGTTTGGTGAAGCAGTGAAAGCGCTCCTAAGTACCAAGTCGTCTGATGGAGCTGCTATTTTAAGAGTTCTGCAGGCATTAAGCTCCCTTGTTGCCTCACTGATTGAGAAAGAGAAGGATCCACAGATTCTTGCAGATAAGGAGCACACTACTCTTTCTCAGGTTTGGGATATCAATGCAGCTTTGGAGCCTTTGTGGCTTGAGCTAAGCAGTTGCATAAGTAAGATAGAAAGCTATTCAGAAACCACCCCTGATTTGTTGACTTCATATAGAGCTTCCACATCTAAACCATCTGGTGTAATTCCTCCCCTGCCTGCTGGGACTCAAAATATTTTGCCATACATAGAATCTTTCTTTGTGGTTTGTGAGAAATTGCATCCTGGGCAGCCAGGCCCTGGTAACGACTTCAGCGTTGCTGCAGTTTCTGATGTTGATGATGCCAGTACATCTGCTAGCCTGCAGAAAACCTCGGGTCCCACTGTGAAAGTGGATGAGAAACATGTTGCTTTTATGAAGTTCTCAGAGAAACATAGGAAGTTGCTAAATGCTTTCATCCGTCAAAATCCTGGTTTGCTAGAAAAGTCTTTCTCGCTGATGCTGAAGGTTCCTCGCTTTATTGATTTTGACAACAAACGTGCTCACTTCAGGTCAAAGATTAAGCATCAACATGATCATCATCACAATCCTCTAAGAATTTCTGTAAGAAGAGCATACATTCTTGAAGATTCATATAACCAGCTTCGCATGCGGTCAGCTCTAGATTTGAAGGGGAGATTGACTGTTCACTTTCAAGGAGAGGAAGGAATTGATGCTGGTGGACTCACGAGGGAATGGTATCAGTTGCTGTCCAGGGTTATCTTTGACAAGGGAGCACTACTATTTACAACAGTGGGCAATGAATCAACTTTCCAACCCAACCCTAACTCTGTTTACCAGACAGAACATCTTTCATATTTCAAGTTTGTTGGGCGAGTGGTTGGGAAAGCACTATTCGATGGTCAATTATTGGATGTCCATTTTACTCGATCCTTCTACAAGCACATTTTGGGGGCTAAGGTGACTTATCATGATATTGAGGCCATTGATCCTGATTACTTTAAAAACCTCAAGTGGATGCTCGAGAATGATATAAGTGATGTTCTAGACCTTACTTTCAGTATTGATGCAGATGAGGAGAAACTAATATTATATGAAAGAACAGAAGTAATGCAATTTCTCTTCTCCTTAAACAGAATTTTTTGTCCCAATATGACTTCTGTGTATTAA

Protein Analysis

3501

Amino Acids

384.91

Weight (kDa)

5.12

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF908 PF06012 107 - 410 1.2e-31 Domain of Unknown Function (DUF908)
DUF913 PF06025 473 - 803 1.6e-59 Domain of Unknown Function (DUF913)
UBA PF00627 1298 - 1335 3e-09 UBA/TS-N domain
UBA_7 PF22562 1299 - 1345 3.8e-14 UBA-like domain
UBM PF14377 2588 - 2611 3.2e-07 Ubiquitin binding region
UBM PF14377 2622 - 2650 2.8e-11 Ubiquitin binding region
UBM PF14377 2659 - 2690 9.6e-07 Ubiquitin binding region
HECT PF00632 3361 - 3470 2.2e-23 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 782, 8136
AarI CACCTGC 1 cut(s) 4522
AasI GACNNNNNNGTC 1 cut(s) 253
AatII GACGTC 1 cut(s) 6823
Acc16I TGCGCA 1 cut(s) 9239
Acc36I ACCTGC 4 cut(s) 921, 4227, 4522, 9057
Acc65I GGTACC 1 cut(s) 856
AccB1I GGYRCC 3 cut(s) 856, 5498, 7032
AccB7I CCANNNNNTGG 5 cut(s) 2311, 5782, 6034, 6211, 9635
AccBSI CCGCTC 1 cut(s) 1147
AccI GTMKAC 6 cut(s) 3193, 3370, 3777, 4163, 5354, 6430
AccII CGCG 6 cut(s) 2982, 3696, 4290, 5710, 8115, 8526
AclI AACGTT 1 cut(s) 1740
AcyI GRCGYC 3 cut(s) 6820, 7033, 8183
AdeI CACNNNGTG 1 cut(s) 7580
AfeI AGCGCT 1 cut(s) 9264
AflIII ACRYGT 4 cut(s) 6109, 7371, 8106, 9739
AhlI ACTAGT 1 cut(s) 2987
AjuI GAANNNNNNNTTGG 4 cut(s) 1804, 1836, 1955, 1987
AleI CACNNNNGTG 1 cut(s) 1903
Alw44I GTGCAC 1 cut(s) 4050
Ama87I CYCGRG 5 cut(s) 65, 6396, 7596, 9709, 10358
Aor51HI AGCGCT 1 cut(s) 9264
ApaLI GTGCAC 1 cut(s) 4050
ArsI GACNNNNNNTTYG 6 cut(s) 4234, 4266, 6096, 6128, 6617, 6649
AseI ATTAAT 1 cut(s) 6726
Asp700I GAANNNNTTC 8 cut(s) 641, 691, 3004, 4131, 4993, 6783, 8040, 9036
Asp718I GGTACC 1 cut(s) 856
AspA2I CCTAGG 1 cut(s) 6932
AsuC2I CCSGG 1 cut(s) 7697
AsuII TTCGAA 2 cut(s) 4661, 8326
AsuNHI GCTAGC 3 cut(s) 2167, 9190, 9692
AvaI CYCGRG 5 cut(s) 65, 6396, 7596, 9709, 10358
AvrII CCTAGG 1 cut(s) 6932
AxyI CCTNAGG 3 cut(s) 2522, 7791, 8530
BaeGI GKGCMC 5 cut(s) 1153, 4054, 4488, 5503, 7293
BaeI ACNNNNGTAYC 6 cut(s) 6384, 6417, 7293, 7326, 7943, 7976
BamHI GGATCC 7 cut(s) 3971, 4909, 5041, 6200, 7556, 8201, 9358
BanI GGYRCC 3 cut(s) 856, 5498, 7032
BanII GRGCYC 5 cut(s) 1117, 3095, 3496, 8281, 8683
BauI CACGAG 6 cut(s) 903, 4047, 6951, 8682, 9090, 10063
BbsI GAAGAC 6 cut(s) 1602, 1835, 2226, 3555, 4038, 6369
BbvCI CCTCAGC 1 cut(s) 9129
BceAI ACGGC 4 cut(s) 10, 55, 79, 6427
BcgI CGANNNNNNTGC 4 cut(s) 4025, 4059, 8105, 8139
BciVI GTATCC 2 cut(s) 6385, 8169
BclI TGATCA 6 cut(s) 2278, 6078, 6166, 6601, 6610, 9910
BcnI CCSGG 1 cut(s) 7697
BcuI ACTAGT 1 cut(s) 2987
BfoI RGCGCY 2 cut(s) 7036, 9266
BfuAI ACCTGC 4 cut(s) 921, 4227, 4522, 9057
BfuI GTATCC 2 cut(s) 6385, 8169
BglI GCCNNNNNGGC 1 cut(s) 9436
BglII AGATCT 5 cut(s) 793, 1682, 4432, 6331, 9218
BlnI CCTAGG 1 cut(s) 6932
BlpI GCTNAGC 2 cut(s) 6252, 9446
BmcAI AGTACT 3 cut(s) 327, 763, 2197
BmeT110I CYCGRG 5 cut(s) 65, 6396, 7596, 9709, 10358
BmrI ACTGGG 1 cut(s) 6199
BmtI GCTAGC 3 cut(s) 2171, 9194, 9696
BmuI ACTGGG 1 cut(s) 6199
BoxI GACNNNNGTC 2 cut(s) 3144, 6910
BpiI GAAGAC 6 cut(s) 1602, 1835, 2226, 3555, 4038, 6369
BplI GAGNNNNNCTC 2 cut(s) 6974, 7006
BpmI CTGGAG 7 cut(s) 1202, 1567, 3395, 3919, 6231, 6425, 6725
Bpu10I CCTNAGC 3 cut(s) 8256, 9129, 10293
Bpu1102I GCTNAGC 2 cut(s) 6252, 9446
Bpu14I TTCGAA 2 cut(s) 4661, 8326
BpuEI CTTGAG 6 cut(s) 4925, 6488, 7136, 8892, 9461, 10331
BpuMI CCSGG 1 cut(s) 7697
BsaAI YACGTR 1 cut(s) 8240
BsaBI GATNNNNATC 3 cut(s) 1386, 7878, 9915
BsaHI GRCGYC 3 cut(s) 6820, 7033, 8183
BsaI GGTCTC 4 cut(s) 1925, 3373, 3698, 4209
BsaWI WCCGGW 3 cut(s) 167, 7225, 8863
Bse118I RCCGGY 2 cut(s) 7094, 8083
Bse21I CCTNAGG 3 cut(s) 2522, 7791, 8530
Bse8I GATNNNNATC 3 cut(s) 1386, 7878, 9915
BseJI GATNNNNATC 3 cut(s) 1386, 7878, 9915
BseSI GKGCMC 5 cut(s) 1153, 4054, 4488, 5503, 7293
BseYI CCCAGC 7 cut(s) 177, 561, 1874, 2673, 3163, 4814, 9557
BsgI GTGCAG 4 cut(s) 2478, 7563, 7803, 9030
Bsh1236I CGCG 6 cut(s) 2982, 3696, 4290, 5710, 8115, 8526
Bsh1285I CGRYCG 2 cut(s) 1061, 2980
BshNI GGYRCC 3 cut(s) 856, 5498, 7032
BsiEI CGRYCG 2 cut(s) 1061, 2980
BsiHKCI CYCGRG 5 cut(s) 65, 6396, 7596, 9709, 10358
BsiSI CCGG 7 cut(s) 168, 4118, 7095, 7226, 7696, 8084, 8864
BslFI GGGAC 4 cut(s) 4463, 9574, 9699, 10467
BsmBI CGTCTC 1 cut(s) 102
BsmFI GGGAC 4 cut(s) 4463, 9574, 9699, 10467
BsmI GAATGC 8 cut(s) 727, 2344, 2851, 6877, 6919, 7575, 8568, 9026
Bso31I GGTCTC 4 cut(s) 1925, 3373, 3698, 4209
BsoBI CYCGRG 5 cut(s) 65, 6396, 7596, 9709, 10358
Bsp119I TTCGAA 2 cut(s) 4661, 8326
Bsp1407I TGTACA 2 cut(s) 936, 2416
Bsp1720I GCTNAGC 2 cut(s) 6252, 9446
Bsp19I CCATGG 6 cut(s) 2457, 3507, 3729, 6210, 7036, 9225
BspFNI CGCG 6 cut(s) 2982, 3696, 4290, 5710, 8115, 8526
BspHI TCATGA 8 cut(s) 1141, 1696, 4372, 5266, 6451, 6613, 7531, 10306
BspMAI CTGCAG 8 cut(s) 932, 2263, 4185, 4761, 8361, 9315, 9661, 9702
BspMI ACCTGC 4 cut(s) 921, 4227, 4522, 9057
BspOI GCTAGC 3 cut(s) 2171, 9194, 9696
BspQI GCTCTTC 8 cut(s) 2058, 2521, 2775, 2856, 3482, 7791, 8658, 9940
BspT104I TTCGAA 2 cut(s) 4661, 8326
BspT107I GGYRCC 3 cut(s) 856, 5498, 7032
BspTNI GGTCTC 4 cut(s) 1925, 3373, 3698, 4209
BsrBI CCGCTC 1 cut(s) 1147
BsrFI RCCGGY 2 cut(s) 7094, 8083
BsrGI TGTACA 2 cut(s) 936, 2416
BssAI RCCGGY 2 cut(s) 7094, 8083
BssNAI GTATAC 2 cut(s) 5355, 6431
BssNI GRCGYC 3 cut(s) 6820, 7033, 8183
BssSI CACGAG 6 cut(s) 903, 4047, 6951, 8682, 9090, 10063
BssT1I CCWWGG 9 cut(s) 1107, 2457, 3507, 3729, 6210, 6932, 7036, 7108, 9225
Bst1107I GTATAC 2 cut(s) 5355, 6431
Bst2BI CACGAG 6 cut(s) 903, 4047, 6951, 8682, 9090, 10063
BstACI GRCGYC 3 cut(s) 6820, 7033, 8183
BstAPI GCANNNNNTGC 5 cut(s) 948, 1103, 3257, 5522, 5573
BstAUI TGTACA 2 cut(s) 936, 2416
BstBAI YACGTR 1 cut(s) 8240
BstBI TTCGAA 2 cut(s) 4661, 8326
BstDSI CCRYGG 7 cut(s) 2457, 3234, 3507, 3729, 6210, 7036, 9225
BstEII GGTNACC 1 cut(s) 6097
BstENI CCTNNNNNAGG 3 cut(s) 2238, 4925, 8239
BstFNI CGCG 6 cut(s) 2982, 3696, 4290, 5710, 8115, 8526
BstH2I RGCGCY 2 cut(s) 7036, 9266
BstMCI CGRYCG 2 cut(s) 1061, 2980
BstPAI GACNNNNGTC 2 cut(s) 3144, 6910
BstPI GGTNACC 1 cut(s) 6097
BstSLI GKGCMC 5 cut(s) 1153, 4054, 4488, 5503, 7293
BstUI CGCG 6 cut(s) 2982, 3696, 4290, 5710, 8115, 8526
BstV2I GAAGAC 6 cut(s) 1602, 1835, 2226, 3555, 4038, 6369
BstXI CCANNNNNNTGG 7 cut(s) 1951, 3982, 4066, 4247, 6287, 7079, 7460
BstZ17I GTATAC 2 cut(s) 5355, 6431
Bsu36I CCTNAGG 3 cut(s) 2522, 7791, 8530
BsuI GTATCC 2 cut(s) 6385, 8169
BtgI CCRYGG 7 cut(s) 2457, 3234, 3507, 3729, 6210, 7036, 9225
BtgZI GCGATG 2 cut(s) 3711, 5282
BtsI GCAGTG 2 cut(s) 3750, 9261
BveI ACCTGC 4 cut(s) 921, 4227, 4522, 9057
CciI TCATGA 8 cut(s) 1141, 1696, 4372, 5266, 6451, 6613, 7531, 10306
Cfr10I RCCGGY 2 cut(s) 7094, 8083
CseI GACGC 5 cut(s) 4279, 5615, 5964, 7430, 8172
CsiI ACCWGGT 1 cut(s) 3609
CspCI CAANNNNNGTGG 4 cut(s) 2040, 2075, 3216, 3251
DinI GGCGCC 1 cut(s) 7034
DraI TTTAAA 3 cut(s) 822, 1474, 10339
DraIII CACNNNGTG 1 cut(s) 7580
DrdI GACNNNNNNGTC 1 cut(s) 253
DseDI GACNNNNNNGTC 1 cut(s) 253
EciI GGCGGA 1 cut(s) 40
Ecl136II GAGCTC 3 cut(s) 1115, 3093, 8279
Eco130I CCWWGG 9 cut(s) 1107, 2457, 3507, 3729, 6210, 6932, 7036, 7108, 9225
Eco147I AGGCCT 5 cut(s) 4261, 5104, 7243, 8229, 8245
Eco24I GRGCYC 5 cut(s) 1117, 3095, 3496, 8281, 8683
Eco31I GGTCTC 4 cut(s) 1925, 3373, 3698, 4209
Eco32I GATATC 4 cut(s) 7912, 8473, 8738, 9412
Eco47III AGCGCT 1 cut(s) 9264
Eco53kI GAGCTC 3 cut(s) 1115, 3093, 8279
Eco81I CCTNAGG 3 cut(s) 2522, 7791, 8530
Eco88I CYCGRG 5 cut(s) 65, 6396, 7596, 9709, 10358
Eco91I GGTNACC 1 cut(s) 6097
EcoICRI GAGCTC 3 cut(s) 1115, 3093, 8279
EcoNI CCTNNNNNAGG 3 cut(s) 2238, 4925, 8239
EcoO109I RGGNCCY 3 cut(s) 3718, 9632, 9713
EcoO65I GGTNACC 1 cut(s) 6097
EcoRI GAATTC 5 cut(s) 4309, 4549, 7627, 7886, 8415
EcoRV GATATC 4 cut(s) 7912, 8473, 8738, 9412
EcoT14I CCWWGG 9 cut(s) 1107, 2457, 3507, 3729, 6210, 6932, 7036, 7108, 9225
EcoT22I ATGCAT 3 cut(s) 498, 4749, 6378
EcoT38I GRGCYC 5 cut(s) 1117, 3095, 3496, 8281, 8683
EgeI GGCGCC 1 cut(s) 7034
EheI GGCGCC 1 cut(s) 7034
ErhI CCWWGG 9 cut(s) 1107, 2457, 3507, 3729, 6210, 6932, 7036, 7108, 9225
Esp3I CGTCTC 1 cut(s) 102
FaqI GGGAC 4 cut(s) 4463, 9574, 9699, 10467
FauI CCCGC 3 cut(s) 1140, 5009, 7425
FauNDI CATATG 1 cut(s) 1660
FbaI TGATCA 6 cut(s) 2278, 6078, 6166, 6601, 6610, 9910
FblI GTMKAC 6 cut(s) 3193, 3370, 3777, 4163, 5354, 6430
FriOI GRGCYC 5 cut(s) 1117, 3095, 3496, 8281, 8683
FspI TGCGCA 1 cut(s) 9239
GsaI CCCAGC 7 cut(s) 181, 565, 1878, 2677, 3167, 4818, 9561
GsuI CTGGAG 7 cut(s) 1202, 1567, 3395, 3919, 6231, 6425, 6725
HaeII RGCGCY 2 cut(s) 7036, 9266
HapII CCGG 7 cut(s) 168, 4118, 7095, 7226, 7696, 8084, 8864
HgaI GACGC 5 cut(s) 4279, 5615, 5964, 7430, 8172
Hin1I GRCGYC 3 cut(s) 6820, 7033, 8183
HincII GTYRAC 8 cut(s) 1993, 3194, 3778, 6193, 7312, 7979, 8049, 9501
HindII GTYRAC 8 cut(s) 1993, 3194, 3778, 6193, 7312, 7979, 8049, 9501
HpaI GTTAAC 1 cut(s) 8049
HpaII CCGG 7 cut(s) 168, 4118, 7095, 7226, 7696, 8084, 8864
Hpy99I CGWCG 3 cut(s) 59, 3195, 3198
HpyCH4IV ACGT 6 cut(s) 1740, 6343, 6820, 8239, 8610, 9874
HpySE526I ACGT 6 cut(s) 1740, 6343, 6820, 8239, 8610, 9874
Hsp92I GRCGYC 3 cut(s) 6820, 7033, 8183
KasI GGCGCC 1 cut(s) 7032
KflI GGGWCCC 1 cut(s) 9713
KpnI GGTACC 1 cut(s) 860
Ksp22I TGATCA 6 cut(s) 2278, 6078, 6166, 6601, 6610, 9910
KspAI GTTAAC 1 cut(s) 8049
LguI GCTCTTC 8 cut(s) 2058, 2521, 2775, 2856, 3482, 7791, 8658, 9940
MabI ACCWGGT 1 cut(s) 3609
MaeII ACGT 6 cut(s) 1740, 6343, 6820, 8239, 8610, 9874
MbiI CCGCTC 1 cut(s) 1147
MfeI CAATTG 5 cut(s) 3409, 3470, 7136, 7761, 9153
Mly113I GGCGCC 1 cut(s) 7033
MmeI TCCRAC 9 cut(s) 1041, 1280, 1382, 2529, 3949, 5986, 6883, 8941, 10174
Mph1103I ATGCAT 3 cut(s) 498, 4749, 6378
MroXI GAANNNNTTC 8 cut(s) 641, 691, 3004, 4131, 4993, 6783, 8040, 9036
MspA1I CMGCKG 9 cut(s) 177, 1460, 2673, 3221, 4762, 6251, 7235, 7427, 8356
MspI CCGG 7 cut(s) 168, 4118, 7095, 7226, 7696, 8084, 8864
MunI CAATTG 5 cut(s) 3409, 3470, 7136, 7761, 9153
Mva1269I GAATGC 8 cut(s) 727, 2344, 2851, 6877, 6919, 7575, 8568, 9026
MvnI CGCG 6 cut(s) 2982, 3696, 4290, 5710, 8115, 8526
NarI GGCGCC 1 cut(s) 7033
NciI CCSGG 1 cut(s) 7697
NcoI CCATGG 6 cut(s) 2457, 3507, 3729, 6210, 7036, 9225
NdeI CATATG 1 cut(s) 1660
NheI GCTAGC 3 cut(s) 2167, 9190, 9692
NmeAIII GCCGAG 3 cut(s) 4588, 8575, 8879
NmuCI GTSAC 6 cut(s) 1757, 1777, 3113, 5129, 6304, 10297
NsbI TGCGCA 1 cut(s) 9239
NsiI ATGCAT 3 cut(s) 498, 4749, 6378
NspV TTCGAA 2 cut(s) 4661, 8326
OliI CACNNNNGTG 1 cut(s) 1903
PaeI GCATGC 4 cut(s) 3290, 4751, 8309, 9988
PaeR7I CTCGAG 2 cut(s) 7596, 10358
PagI TCATGA 8 cut(s) 1141, 1696, 4372, 5266, 6451, 6613, 7531, 10306
PaqCI CACCTGC 1 cut(s) 4522
PceI AGGCCT 5 cut(s) 4261, 5104, 7243, 8229, 8245
PciI ACATGT 4 cut(s) 6109, 7371, 8106, 9739
PciSI GCTCTTC 8 cut(s) 2058, 2521, 2775, 2856, 3482, 7791, 8658, 9940
PcsI WCGNNNNNNNCGW 4 cut(s) 910, 8137, 8323, 9648
PctI GAATGC 8 cut(s) 727, 2344, 2851, 6877, 6919, 7575, 8568, 9026
PdmI GAANNNNTTC 8 cut(s) 641, 691, 3004, 4131, 4993, 6783, 8040, 9036
PflFI GACNNNGTC 1 cut(s) 7044
PflMI CCANNNNNTGG 5 cut(s) 2311, 5782, 6034, 6211, 9635
PfoI TCCNGGA 1 cut(s) 29
PluTI GGCGCC 1 cut(s) 7036
Ppu21I YACGTR 1 cut(s) 8240
PpuMI RGGWCCY 2 cut(s) 3718, 9713
PscI ACATGT 4 cut(s) 6109, 7371, 8106, 9739
PshAI GACNNNNGTC 2 cut(s) 3144, 6910
PshBI ATTAAT 1 cut(s) 6726
PsiI TTATAA 2 cut(s) 782, 8136
Psp124BI GAGCTC 3 cut(s) 1117, 3095, 8281
Psp1406I AACGTT 1 cut(s) 1740
Psp5II RGGWCCY 2 cut(s) 3718, 9713
PspEI GGTNACC 1 cut(s) 6097
PspFI CCCAGC 7 cut(s) 177, 561, 1874, 2673, 3163, 4814, 9557
PspPPI RGGWCCY 2 cut(s) 3718, 9713
PspXI VCTCGAGB 1 cut(s) 7596
PsrI GAACNNNNNNTAC 4 cut(s) 1863, 1895, 3868, 3900
PstI CTGCAG 8 cut(s) 932, 2263, 4185, 4761, 8361, 9315, 9661, 9702
PsyI GACNNNGTC 1 cut(s) 7044
PvuII CAGCTG 6 cut(s) 1460, 4762, 6251, 7235, 7427, 8356
SacI GAGCTC 3 cut(s) 1117, 3095, 8281
SalI GTCGAC 2 cut(s) 3192, 3776
SapI GCTCTTC 8 cut(s) 2058, 2521, 2775, 2856, 3482, 7791, 8658, 9940
ScaI AGTACT 3 cut(s) 327, 763, 2197
SexAI ACCWGGT 1 cut(s) 3609
SfoI GGCGCC 1 cut(s) 7034
Sfr274I CTCGAG 2 cut(s) 7596, 10358
SfuI TTCGAA 2 cut(s) 4661, 8326
SgrDI CGTCGACG 1 cut(s) 3192
SlaI CTCGAG 2 cut(s) 7596, 10358
SmlI CTYRAG 8 cut(s) 4904, 6467, 7115, 7596, 8907, 9440, 10346, 10358
SmoI CTYRAG 8 cut(s) 4904, 6467, 7115, 7596, 8907, 9440, 10346, 10358
SpeI ACTAGT 1 cut(s) 2987
SphI GCATGC 4 cut(s) 3290, 4751, 8309, 9988
SseBI AGGCCT 5 cut(s) 4261, 5104, 7243, 8229, 8245
SspDI GGCGCC 1 cut(s) 7032
SspI AATATT 5 cut(s) 1336, 4876, 5673, 9571, 10425
SstI GAGCTC 3 cut(s) 1117, 3095, 8281
StuI AGGCCT 5 cut(s) 4261, 5104, 7243, 8229, 8245
StyI CCWWGG 9 cut(s) 1107, 2457, 3507, 3729, 6210, 6932, 7036, 7108, 9225
TaiI ACGT 6 cut(s) 1743, 6346, 6823, 8242, 8613, 9877
TaqII GACCGA 1 cut(s) 2966
TauI GCSGC 4 cut(s) 217, 2564, 6878, 8568
TseFI GTSAC 6 cut(s) 1757, 1777, 3113, 5129, 6304, 10297
Tsp45I GTSAC 6 cut(s) 1757, 1777, 3113, 5129, 6304, 10297
TspGWI ACGGA 7 cut(s) 902, 3006, 3223, 3567, 4883, 8941, 9785
Tth111I GACNNNGTC 1 cut(s) 7044
Van91I CCANNNNNTGG 5 cut(s) 2311, 5782, 6034, 6211, 9635
VneI GTGCAC 1 cut(s) 4050
VspI ATTAAT 1 cut(s) 6726
XagI CCTNNNNNAGG 3 cut(s) 2238, 4925, 8239
XbaI TCTAGA 6 cut(s) 2773, 5341, 6088, 6815, 9997, 10381
XcmI CCANNNNNNNNNTGG 5 cut(s) 2791, 6287, 7079, 7460, 9724
XhoI CTCGAG 2 cut(s) 7596, 10358
XmaJI CCTAGG 1 cut(s) 6932
XmiI GTMKAC 6 cut(s) 3193, 3370, 3777, 4163, 5354, 6430
XmnI GAANNNNTTC 8 cut(s) 641, 691, 3004, 4131, 4993, 6783, 8040, 9036
ZraI GACGTC 1 cut(s) 6821
ZrmI AGTACT 3 cut(s) 327, 763, 2197
Zsp2I ATGCAT 3 cut(s) 498, 4749, 6378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.