MD08G1215300.v1.1

Nuclear pore complex protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004174
Physical Location & Seq
Forward (+)
27827088 .. 27833504
6417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1215300.v1.1.491

Sequence Viewer

Length: 3096 bp
ATGGGCATTGATTCTGGAACTCCTAATTCACAAGTAGCATGTCAACACAAGAAACGAAGGGTCTCTCGGGATGCTGGCATTTCTTTGTCTGAGACTTTTAGTCGCCTTGAAGGATCCTTAACTTATTTACCAACTTTAGAAGCTGCTGATTACTATACACATCCCTCTTTGAAGGATTTGGCTGCTCGGGAATATGCAGATCCTGGTTTTTGTAGCCGAGTGTTGGACTTCACAGTTGGGAGACTCGGTCATGGATCTGTCAAGTATCCTGGGAAGACTGATATTAGGTGTTTGAAGTTAGATAATATTGTCAAGTTCCACAGACATGAGGTAATCGTGTATGACGATGAAGCTGTCAAGCCTTTGGTTGGCCAAGGCCTTAACAAGCCTGCTGAAGTAACTTTGGTGCTACAAACAAGACCGTCAAATATTGACAAGAGACAGAAAGATAATATTGTGAAAAAGTTGAGGAAGAGTGTGGAGGGACAAGGAGCTCGCTTTATTTCGTTTAATCCAGAAAGCGGGGAATGGAAATTCTTTGTCCACCATTTCAGCAGATTTGGGTTGAATGAAGATGATGAAGAAGACATCATGATGGAAGATACTGCTTCAGCCCAAGATCTTGTGGAAATGAACAATGGTGATATTTCTGATGTTGATGAAGAAAACCAAATAGATCCCACTGGAGTTGTGCTGTCACATTCTCTTCCTGCTCATCTTGGGCTTGACCCAGTAAAGATGAAAGAAATGAGAATGTTGATGTTTCATGATGAGGAAGCGGAGGCTGAGGATTTGAATCATATCCCTGCACATTATAATCCATCCTTTGCTTCTCAGAGGATGAGTCAGAGATCTACTCCACCAGCTGTGCGAAAAACCCCGCTGGCATTACTTGAGTACAAGCATGGTAGTTTTGACTCAAATTCTCCTGGAGCCATTCTAATGGCCCAGGAAAATAAGGCCATGCCCCTTAAGACATTGAAAGAAGGTTTTCAGCTTGATCTCAAGCATGAAACACCAGTAACCAGAAAACATTCTTGCAACATAGTTGATGCAGGTCTGTTTATGGGTAGGTCATTTCGAGTAGGATGGGGCCCAAACGGAATCCTTGTGCATGCTGGAACACCAGTAGGGAGTAACGGTTCTCCAAAGATGCTGTCATCCATAGTCAATATAGAGAAGGTTGCTATTGACAGTGTTGTTCGAGATGAAAACAACAAAGTTAGGGAGGAACTTGTTGACATGGCTATTGATTCTCCATTAGATCTTCACAAGAGAATATCTCATCAGACAAAGGAGATTGAACTTGGACCATTCAACCTGAGGTTTCAAAAGCTTGTCTCCAACCGCTTGATGCTTACACAGATTTGCAGGAGCTATGTAGATATTATTGAGAAGCAGCTGGAAGTTCCTGGCCTATCTTCATCTTACCGTTTGGTTTTGACACACCAAATAATGATTTGGGAACTGATTAAAGTTCTCTTTTCTGATAGGGAAAATGGTGGAAGATCAAAATCCTTGGGTGCTGATAGTGAGGAAGAAATGGTGCAGGATGTGAAGGAGGCTTCTCAACAAGTCGACCTGGAAGCTTTACCTCTTATTCGGAGGGCAGAGTTTAGCTGTTGGTTGCAAGAGAATGTTTCCCATCGAGTACAAGAAAAGATAAGCTCCTTGAATGAGTCCAGCTATCTGGAATATATATTATTACTTTTGAGTGGGCGTCAGCTGGATGCTGCTGTGGAAATTGCTGCTTCTCAGGGAGATGTAAGATTGGCTTGTTTGTTGAGTCAGGCCGGTGGGTCCATAGTCAATCGTACTGATGTTGCTGAACAACTTGATCGTTGGAGGATCAATGGGCTCGATTTCAACTTCATTGAGAAAGACAGGATAAGGCTTTATGAGTTGCTTGCTGGTAATATCCATGGTGCTTTTCATGATGTTAACATTGATTGGAAAAGGTTTCTAGGTTTGTTGATGTGGTATCAATTAGAACCTAACACTTCATTACCCAAAATATTTCGCACTTATCAACATCTTCTTGATGATTGTAAGGCTCCATACCCTGTTCCTGTCTACATTGATGAAGGACTGGTAGAAGAGGCTGAGGATTTGAATGTGGTGAAACGTTATGACCTATCGTATTATCTGATGCTGCTTCATGCCAGTGAAGAAAGTGAAGTTGGCTTTCTAAAGTCCATGTTTAGTGCCTTCTCGTCAACACATGATCCACTTGATTACCATATGATCTGGCATCAGCGTGCAGTGTTGGAATCAGTTGGGGCTATCAGTTCTAAGGATCTTCATGTTCTTGACATGGGATTTGTTTCCCAGCTGTTGTGTCTTGGGCAATGTCATTGGGCGATCTATGTGGTCCTTCACATGCCCCATTGTGAAGATTTTCCATATCTTCACTCTAATCTTATTCGGGAAATCTTGTTCCAATACTGTGAATCCTGGAGTTCTCAAGAATCACAACGCCAAGCCATTGAGGACTTGGGTATTCCTAAGGCATGGCTTCATGAGGCTATGGCTGTTTATTTCAATTACTATGGGGAACTTGCGAAGGCTCTTGAACACTTTCTTGAATGTGCAAATTGGCAGAGAGCGCATGCTATTTTCGTAACTTCTGTTGCTCACAAATTATTCTTGTCTGTCGAACACTCAGAAATATGGAGGCTTGCAACTTCCATGGAGGACCACAAATCAGAAATTGAAAATTGGGACCTCGGAGCTGGGATTTATATTTCATTCTATTCAATTAGAAGTTCATTGCAGGAAGTGAACGATACCATGAATGAATTGGATTCACTTGAAAGCAGAAATTCTGCTTGTAGGGAATTTCTTGGTCAGTTAAACCAGTCTTTGGCTGTTTGGGGTGTCAGGTTACCAATTGACGTGAGGGTAGTGTATTCGAAGATGGCAGACGAGATATGCAGTTTGCTTTTATCCGACATTGGCGAGGGACCAACACGAGATGTTCAGTTAAGTTGCTTTGACACCGTTTTTAGTGCTCCCATTCCTGAAGACAAACGCTCGAGTCATTTGCAAGAGGCAGTGTCTCTCTTTACATGTTTTCTTTCGGAGGCTACGACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000972 GO:0000973 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003682 GO:0003712 GO:0003713 GO:0003723 GO:0003729 GO:0005048 GO:0005198 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006403 GO:0006405 GO:0006406 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0006999 GO:0007154 GO:0007165 GO:0008104 GO:0008139 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009733 GO:0009870 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015267 GO:0015288 GO:0015833 GO:0015931 GO:0016020 GO:0016032 GO:0016043 GO:0016234 GO:0016604 GO:0017038 GO:0017056 GO:0019219 GO:0019222 GO:0022607 GO:0022803 GO:0022829 GO:0022857 GO:0023052 GO:0031080 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0033036 GO:0033120 GO:0033218 GO:0033365 GO:0034397 GO:0034398 GO:0034399 GO:0034504 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042277 GO:0042405 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044614 GO:0044615 GO:0045088 GO:0045089 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0046931 GO:0048024 GO:0048026 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050000 GO:0050657 GO:0050658 GO:0050684 GO:0050685 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051276 GO:0051292 GO:0051640 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0055085 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0080134 GO:0090304 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901576 GO:1902680 GO:1903311 GO:1903313 GO:1903506 GO:1903508 GO:1990841 GO:1990904 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
Nucleoporin2 Nup96

Protein Analysis

1032

Amino Acids

116.83

Weight (kDa)

5.32

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleoporin2 PF04096 49 - 189 3.4e-37 Nup98-96 autopeptidase S59
Nup96 PF12110 566 - 846 1e-77 Nuclear protein 96
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 816
Acc36I ACCTGC 1 cut(s) 1046
AccB7I CCANNNNNTGG 1 cut(s) 2863
AccI GTMKAC 2 cut(s) 1578, 2073
AciI CCGC 4 cut(s) 522, 779, 881, 1348
AclI AACGTT 1 cut(s) 2125
AclWI GGATC 8 cut(s) 108, 121, 194, 262, 671, 1856, 2219, 2304
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 4 cut(s) 533, 922, 2821, 2837
AcuI CTGAAG 3 cut(s) 414, 594, 3042
AcyI GRCGYC 1 cut(s) 1720
AfaI GTAC 3 cut(s) 899, 1653, 1816
AfiI CCNNNNNNNGG 4 cut(s) 223, 368, 521, 2863
AflII CTTAAG 1 cut(s) 971
AflIII ACRYGT 1 cut(s) 3068
AhdI GACNNNNNGTC 1 cut(s) 99
AjiI CACGTC 1 cut(s) 2896
AjnI CCWGG 7 cut(s) 202, 268, 928, 948, 1411, 1581, 2453
AjuI GAANNNNNNNTTGG 2 cut(s) 2163, 2195
AloI GAACNNNNNNTCC 4 cut(s) 1126, 1158, 2420, 2452
Alw21I GWGCWC 2 cut(s) 496, 3013
Alw26I GTCTC 6 cut(s) 67, 86, 235, 433, 1345, 3063
AlwI GGATC 8 cut(s) 108, 121, 194, 262, 671, 1856, 2219, 2304
AlwNI CAGNNNCTG 1 cut(s) 203
Ama87I CYCGRG 3 cut(s) 66, 186, 3034
AoxI GGCC 7 cut(s) 370, 376, 945, 960, 1093, 1414, 1791
ApaI GGGCCC 1 cut(s) 1097
ApeKI GCWGC 6 cut(s) 143, 182, 1399, 1733, 1748, 2152
ApoI RAATTY 4 cut(s) 533, 922, 2821, 2837
ArsI GACNNNNNNTTYG 4 cut(s) 1142, 1174, 2303, 2335
Asp700I GAANNNNTTC 4 cut(s) 990, 1033, 2397, 2577
AspLEI GCGC 1 cut(s) 2608
AspS9I GGNCC 9 cut(s) 946, 1093, 1094, 1310, 1800, 2371, 2695, 2722, 2963
AsuHPI GGTGA 2 cut(s) 653, 2131
AsuII TTCGAA 1 cut(s) 2912
AvaI CYCGRG 3 cut(s) 66, 186, 3034
AvaII GGWCC 6 cut(s) 1310, 1800, 2371, 2695, 2722, 2963
AxyI CCTNAGG 2 cut(s) 1322, 2505
BaeGI GKGCMC 1 cut(s) 1097
BalI TGGCCA 1 cut(s) 372
BamHI GGATCC 1 cut(s) 113
BanII GRGCYC 3 cut(s) 496, 1097, 1860
BauI CACGAG 1 cut(s) 2970
BbsI GAAGAC 3 cut(s) 281, 591, 3030
Bbv12I GWGCWC 2 cut(s) 496, 3013
BbvCI CCTCAGC 2 cut(s) 786, 2103
BbvI GCAGC 6 cut(s) 130, 169, 1411, 1720, 1735, 2139
BccI CCATC 5 cut(s) 589, 829, 1083, 1653, 2911
BcgI CGANNNNNNTGC 2 cut(s) 3025, 3059
BciT130I CCWGG 7 cut(s) 204, 270, 930, 950, 1413, 1583, 2455
BciVI GTATCC 1 cut(s) 276
BcoDI GTCTC 6 cut(s) 67, 86, 235, 433, 1345, 3063
BfaI CTAG 1 cut(s) 1964
BfrI CTTAAG 1 cut(s) 971
BfuAI ACCTGC 1 cut(s) 1046
BfuI GTATCC 1 cut(s) 276
BglII AGATCT 3 cut(s) 619, 851, 1264
BisI GCNGC 6 cut(s) 144, 183, 1400, 1734, 1749, 2153
BlsI GCNGC 6 cut(s) 145, 184, 1401, 1735, 1750, 2154
Bme1390I CCNGG 7 cut(s) 204, 270, 930, 950, 1413, 1583, 2455
Bme18I GGWCC 6 cut(s) 1310, 1800, 2371, 2695, 2722, 2963
BmeRI GACNNNNNGTC 1 cut(s) 99
BmeT110I CYCGRG 3 cut(s) 66, 186, 3034
BmgBI CACGTC 1 cut(s) 2896
BmgT120I GGNCC 9 cut(s) 946, 1093, 1094, 1310, 1800, 2371, 2695, 2722, 2963
BmiI GGNNCC 8 cut(s) 115, 934, 1094, 1095, 1801, 2055, 2723, 2964
BmrFI CCNGG 7 cut(s) 204, 270, 930, 950, 1413, 1583, 2455
BmrI ACTGGG 1 cut(s) 725
BmsI GCATC 7 cut(s) 61, 1042, 1143, 1344, 1720, 2139, 2260
BmuI ACTGGG 1 cut(s) 725
BpiI GAAGAC 3 cut(s) 281, 591, 3030
BplI GAGNNNNNCTC 4 cut(s) 841, 873, 1267, 1299
BpmI CTGGAG 3 cut(s) 705, 951, 2476
Bpu10I CCTNAGC 2 cut(s) 786, 2103
Bpu14I TTCGAA 1 cut(s) 2912
BpuEI CTTGAG 3 cut(s) 914, 989, 2448
BsaBI GATNNNNATC 2 cut(s) 795, 1513
BsaHI GRCGYC 1 cut(s) 1720
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 7 cut(s) 269, 373, 948, 1518, 1921, 2688, 2725
BsaXI ACNNNNNCTCC 4 cut(s) 232, 262, 1126, 1156
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 368, 521, 2863
Bse118I RCCGGY 1 cut(s) 1793
Bse1I ACTGG 7 cut(s) 688, 731, 1019, 1127, 2094, 2163, 2857
Bse21I CCTNAGG 2 cut(s) 1322, 2505
Bse3DI GCAATG 2 cut(s) 2354, 2768
Bse8I GATNNNNATC 2 cut(s) 795, 1513
BseBI CCWGG 7 cut(s) 204, 270, 930, 950, 1413, 1583, 2455
BseDI CCNNGG 7 cut(s) 269, 373, 948, 1518, 1921, 2688, 2725
BseGI GGATG 8 cut(s) 76, 160, 821, 846, 1094, 1160, 1558, 1735
BseJI GATNNNNATC 2 cut(s) 795, 1513
BseLI CCNNNNNNNGG 4 cut(s) 223, 368, 521, 2863
BseMI GCAATG 2 cut(s) 2354, 2768
BseMII CTCAG 7 cut(s) 81, 777, 848, 1313, 1769, 2094, 2676
BseNI ACTGG 7 cut(s) 688, 731, 1019, 1127, 2094, 2163, 2857
BseSI GKGCMC 1 cut(s) 1097
BseXI GCAGC 6 cut(s) 130, 169, 1411, 1720, 1735, 2139
BseYI CCCAGC 2 cut(s) 2328, 2732
BsgI GTGCAG 3 cut(s) 792, 1568, 2280
BshFI GGCC 7 cut(s) 372, 378, 947, 962, 1095, 1416, 1793
BsiHKAI GWGCWC 2 cut(s) 496, 3013
BsiHKCI CYCGRG 3 cut(s) 66, 186, 3034
BsiSI CCGG 1 cut(s) 1794
BslFI GGGAC 3 cut(s) 498, 2735, 2976
BslI CCNNNNNNNGG 4 cut(s) 223, 368, 521, 2863
BsmAI GTCTC 6 cut(s) 67, 86, 235, 433, 1345, 3063
BsmFI GGGAC 3 cut(s) 498, 2735, 2976
BsnI GGCC 7 cut(s) 372, 378, 947, 962, 1095, 1416, 1793
Bso31I GGTCTC 1 cut(s) 67
BsoBI CYCGRG 3 cut(s) 66, 186, 3034
Bsp119I TTCGAA 1 cut(s) 2912
Bsp120I GGGCCC 1 cut(s) 1093
Bsp1286I GDGCHC 4 cut(s) 496, 1097, 1860, 3013
Bsp19I CCATGG 2 cut(s) 1921, 2688
BspACI CCGC 4 cut(s) 522, 779, 881, 1348
BspANI GGCC 7 cut(s) 372, 378, 947, 962, 1095, 1416, 1793
BspCNI CTCAG 7 cut(s) 82, 778, 847, 1314, 1768, 2095, 2675
BspHI TCATGA 4 cut(s) 591, 766, 1933, 2518
BspLI GGNNCC 8 cut(s) 115, 934, 1094, 1095, 1801, 2055, 2723, 2964
BspMI ACCTGC 1 cut(s) 1046
BspPI GGATC 8 cut(s) 108, 121, 194, 262, 671, 1856, 2219, 2304
BspT104I TTCGAA 1 cut(s) 2912
BspTI CTTAAG 1 cut(s) 971
BspTNI GGTCTC 1 cut(s) 67
BsrDI GCAATG 2 cut(s) 2354, 2768
BsrFI RCCGGY 1 cut(s) 1793
BsrI ACTGG 7 cut(s) 688, 731, 1019, 1127, 2094, 2163, 2857
BssAI RCCGGY 1 cut(s) 1793
BssECI CCNNGG 7 cut(s) 269, 373, 948, 1518, 1921, 2688, 2725
BssNI GRCGYC 1 cut(s) 1720
BssSI CACGAG 1 cut(s) 2970
BssT1I CCWWGG 4 cut(s) 373, 1518, 1921, 2688
Bst2BI CACGAG 1 cut(s) 2970
Bst2UI CCWGG 7 cut(s) 204, 270, 930, 950, 1413, 1583, 2455
Bst4CI ACNGT 7 cut(s) 235, 423, 1142, 1196, 1433, 2447, 3001
Bst6I CTCTTC 3 cut(s) 467, 711, 2091
BstACI GRCGYC 1 cut(s) 1720
BstAFI CTTAAG 1 cut(s) 971
BstBI TTCGAA 1 cut(s) 2912
BstC8I GCNNGC 9 cut(s) 76, 390, 496, 885, 1116, 1908, 2259, 2610, 2679
BstDEI CTNAG 9 cut(s) 90, 786, 834, 1322, 1755, 2103, 2292, 2505, 2662
BstDSI CCRYGG 2 cut(s) 1921, 2688
BstEII GGTNACC 1 cut(s) 2883
BstF5I GGATG 8 cut(s) 76, 160, 821, 846, 1094, 1160, 1558, 1735
BstHHI GCGC 1 cut(s) 2608
BstMAI GTCTC 6 cut(s) 67, 86, 235, 433, 1345, 3063
BstMWI GCNNNNNNNGC 1 cut(s) 2605
BstNI CCWGG 7 cut(s) 204, 270, 930, 950, 1413, 1583, 2455
BstNSI RCATGY 5 cut(s) 42, 1118, 2383, 2612, 3072
BstPI GGTNACC 1 cut(s) 2883
BstSCI CCNGG 7 cut(s) 202, 268, 928, 948, 1411, 1581, 2453
BstSLI GKGCMC 1 cut(s) 1097
BstV1I GCAGC 6 cut(s) 130, 169, 1411, 1720, 1735, 2139
BstV2I GAAGAC 3 cut(s) 281, 591, 3030
BstX2I RGATCY 8 cut(s) 113, 199, 254, 619, 676, 851, 1264, 2296
BstXI CCANNNNNNTGG 2 cut(s) 943, 1690
BstYI RGATCY 8 cut(s) 113, 199, 254, 619, 676, 851, 1264, 2296
Bsu36I CCTNAGG 2 cut(s) 1322, 2505
BsuI GTATCC 1 cut(s) 276
BsuRI GGCC 7 cut(s) 372, 378, 947, 962, 1095, 1416, 1793
BtgI CCRYGG 2 cut(s) 1921, 2688
BtrI CACGTC 1 cut(s) 2896
BtsCI GGATG 8 cut(s) 76, 160, 821, 846, 1094, 1160, 1558, 1735
BtsI GCAGTG 2 cut(s) 2268, 3060
BtsIMutI CAGTG 5 cut(s) 681, 1201, 2170, 2268, 3060
BveI ACCTGC 1 cut(s) 1046
Cac8I GCNNGC 9 cut(s) 76, 390, 496, 885, 1116, 1908, 2259, 2610, 2679
CaiI CAGNNNCTG 1 cut(s) 203
CciI TCATGA 4 cut(s) 591, 766, 1933, 2518
CfoI GCGC 1 cut(s) 2608
Cfr10I RCCGGY 1 cut(s) 1793
Cfr13I GGNCC 9 cut(s) 946, 1093, 1094, 1310, 1800, 2371, 2695, 2722, 2963
CseI GACGC 1 cut(s) 1709
Csp6I GTAC 3 cut(s) 898, 1652, 1815
CspCI CAANNNNNGTGG 2 cut(s) 670, 705
CviQI GTAC 3 cut(s) 898, 1652, 1815
DdeI CTNAG 9 cut(s) 90, 786, 834, 1322, 1755, 2103, 2292, 2505, 2662
DriI GACNNNNNGTC 1 cut(s) 99
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 3 cut(s) 467, 711, 2091
Eam1105I GACNNNNNGTC 1 cut(s) 99
EarI CTCTTC 3 cut(s) 467, 711, 2091
Ecl136II GAGCTC 1 cut(s) 494
Eco130I CCWWGG 4 cut(s) 373, 1518, 1921, 2688
Eco147I AGGCCT 1 cut(s) 378
Eco24I GRGCYC 3 cut(s) 496, 1097, 1860
Eco31I GGTCTC 1 cut(s) 67
Eco47I GGWCC 6 cut(s) 1310, 1800, 2371, 2695, 2722, 2963
Eco53kI GAGCTC 1 cut(s) 494
Eco57I CTGAAG 3 cut(s) 414, 594, 3042
Eco81I CCTNAGG 2 cut(s) 1322, 2505
Eco88I CYCGRG 3 cut(s) 66, 186, 3034
Eco91I GGTNACC 1 cut(s) 2883
EcoICRI GAGCTC 1 cut(s) 494
EcoO109I RGGNCCY 2 cut(s) 1093, 2722
EcoO65I GGTNACC 1 cut(s) 2883
EcoRII CCWGG 7 cut(s) 202, 268, 928, 948, 1411, 1581, 2453
EcoT14I CCWWGG 4 cut(s) 373, 1518, 1921, 2688
EcoT38I GRGCYC 3 cut(s) 496, 1097, 1860
ErhI CCWWGG 4 cut(s) 373, 1518, 1921, 2688
FalI AAGNNNNNCTT 2 cut(s) 1759, 1791
FaqI GGGAC 3 cut(s) 498, 2735, 2976
FauI CCCGC 2 cut(s) 515, 888
FauNDI CATATG 1 cut(s) 2241
FblI GTMKAC 2 cut(s) 1578, 2073
Fnu4HI GCNGC 6 cut(s) 144, 183, 1400, 1734, 1749, 2153
FokI GGATG 8 cut(s) 83, 147, 808, 853, 1101, 1147, 1565, 1742
FriOI GRGCYC 3 cut(s) 496, 1097, 1860
Fsp4HI GCNGC 6 cut(s) 144, 183, 1400, 1734, 1749, 2153
FspBI CTAG 1 cut(s) 1964
GlaI GCGC 1 cut(s) 2607
GluI GCNGC 6 cut(s) 144, 183, 1400, 1734, 1749, 2153
GsaI CCCAGC 2 cut(s) 2332, 2736
GsuI CTGGAG 3 cut(s) 705, 951, 2476
HaeIII GGCC 7 cut(s) 372, 378, 947, 962, 1095, 1416, 1793
HapII CCGG 1 cut(s) 1794
HgaI GACGC 1 cut(s) 1709
HhaI GCGC 1 cut(s) 2608
Hin1I GRCGYC 1 cut(s) 1720
Hin6I GCGC 1 cut(s) 2606
HinP1I GCGC 1 cut(s) 2606
HincII GTYRAC 5 cut(s) 44, 1240, 1579, 1942, 2217
HindII GTYRAC 5 cut(s) 44, 1240, 1579, 1942, 2217
HindIII AAGCTT 2 cut(s) 1334, 1587
HpaI GTTAAC 1 cut(s) 1942
HpaII CCGG 1 cut(s) 1794
HphI GGTGA 2 cut(s) 653, 2131
Hpy166II GTNNAC 8 cut(s) 44, 544, 1240, 1579, 1942, 2074, 2217, 2782
Hpy8I GTNNAC 8 cut(s) 44, 544, 1240, 1579, 1942, 2074, 2217, 2782
Hpy99I CGWCG 1 cut(s) 3094
HpyCH4III ACNGT 7 cut(s) 235, 423, 1142, 1196, 1433, 2447, 3001
HpyCH4IV ACGT 3 cut(s) 2125, 2895, 3092
HpyF10VI GCNNNNNNNGC 1 cut(s) 2605
HpyF3I CTNAG 9 cut(s) 90, 786, 834, 1322, 1755, 2103, 2292, 2505, 2662
HpySE526I ACGT 3 cut(s) 2125, 2895, 3092
Hsp92I GRCGYC 1 cut(s) 1720
HspAI GCGC 1 cut(s) 2606
KspAI GTTAAC 1 cut(s) 1942
LmnI GCTCC 7 cut(s) 491, 932, 1374, 1673, 2059, 2729, 3016
Lsp1109I GCAGC 6 cut(s) 130, 169, 1411, 1720, 1735, 2139
LweI GCATC 7 cut(s) 61, 1042, 1143, 1344, 1720, 2139, 2260
MaeI CTAG 1 cut(s) 1964
MaeII ACGT 3 cut(s) 2125, 2895, 3092
MaeIII GTNAC 6 cut(s) 397, 696, 1021, 1136, 2620, 2883
MfeI CAATTG 1 cut(s) 2889
MflI RGATCY 8 cut(s) 113, 199, 254, 619, 676, 851, 1264, 2296
MhlI GDGCHC 4 cut(s) 496, 1097, 1860, 3013
MlsI TGGCCA 1 cut(s) 372
MluNI TGGCCA 1 cut(s) 372
MlyI GAGTC 6 cut(s) 237, 853, 911, 1688, 1795, 3046
MmeI TCCRAC 5 cut(s) 204, 1368, 1823, 2247, 2973
Mox20I TGGCCA 1 cut(s) 372
MroXI GAANNNNTTC 4 cut(s) 990, 1033, 2397, 2577
MscI TGGCCA 1 cut(s) 372
MseI TTAA 8 cut(s) 119, 381, 510, 972, 1473, 1941, 2852, 2984
MslI CAYNNNNRTG 5 cut(s) 324, 593, 941, 2163, 2256
Msp20I TGGCCA 1 cut(s) 372
MspA1I CMGCKG 5 cut(s) 866, 883, 1402, 1726, 2332
MspCI CTTAAG 1 cut(s) 971
MspI CCGG 1 cut(s) 1794
MspR9I CCNGG 7 cut(s) 204, 270, 930, 950, 1413, 1583, 2455
MunI CAATTG 1 cut(s) 2889
MvaI CCWGG 7 cut(s) 204, 270, 930, 950, 1413, 1583, 2455
MwoI GCNNNNNNNGC 1 cut(s) 2605
NcoI CCATGG 2 cut(s) 1921, 2688
NdeI CATATG 1 cut(s) 2241
NlaIV GGNNCC 8 cut(s) 115, 934, 1094, 1095, 1801, 2055, 2723, 2964
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 1 cut(s) 696
NspI RCATGY 5 cut(s) 42, 1118, 2383, 2612, 3072
NspV TTCGAA 1 cut(s) 2912
PaeI GCATGC 2 cut(s) 1118, 2612
PaeR7I CTCGAG 1 cut(s) 3034
PagI TCATGA 4 cut(s) 591, 766, 1933, 2518
PceI AGGCCT 1 cut(s) 378
PciI ACATGT 1 cut(s) 3068
PcsI WCGNNNNNNNCGW 2 cut(s) 342, 3086
PdmI GAANNNNTTC 4 cut(s) 990, 1033, 2397, 2577
PfeI GAWTC 8 cut(s) 11, 796, 1104, 1253, 2270, 2450, 2468, 2804
PflFI GACNNNGTC 1 cut(s) 246
PflMI CCANNNNNTGG 1 cut(s) 2863
PfoI TCCNGGA 2 cut(s) 928, 2453
PkrI GCNGC 6 cut(s) 145, 184, 1401, 1735, 1750, 2154
PleI GAGTC 6 cut(s) 237, 852, 911, 1687, 1794, 3045
PpsI GAGTC 6 cut(s) 237, 852, 911, 1687, 1794, 3045
PpuMI RGGWCCY 1 cut(s) 2722
PscI ACATGT 1 cut(s) 3068
PsiI TTATAA 1 cut(s) 816
Psp124BI GAGCTC 1 cut(s) 496
Psp1406I AACGTT 1 cut(s) 2125
Psp5II RGGWCCY 1 cut(s) 2722
Psp6I CCWGG 7 cut(s) 202, 268, 928, 948, 1411, 1581, 2453
PspEI GGTNACC 1 cut(s) 2883
PspFI CCCAGC 2 cut(s) 2328, 2732
PspGI CCWGG 7 cut(s) 202, 268, 928, 948, 1411, 1581, 2453
PspN4I GGNNCC 8 cut(s) 115, 934, 1094, 1095, 1801, 2055, 2723, 2964
PspOMI GGGCCC 1 cut(s) 1093
PspPI GGNCC 9 cut(s) 946, 1093, 1094, 1310, 1800, 2371, 2695, 2722, 2963
PspPPI RGGWCCY 1 cut(s) 2722
PspXI VCTCGAGB 1 cut(s) 3034
PstNI CAGNNNCTG 1 cut(s) 203
PsuI RGATCY 8 cut(s) 113, 199, 254, 619, 676, 851, 1264, 2296
PsyI GACNNNGTC 1 cut(s) 246
PvuII CAGCTG 4 cut(s) 866, 1402, 1726, 2332
RsaI GTAC 3 cut(s) 899, 1653, 1816
RsaNI GTAC 3 cut(s) 898, 1652, 1815
RseI CAYNNNNRTG 5 cut(s) 324, 593, 941, 2163, 2256
SacI GAGCTC 1 cut(s) 496
SalI GTCGAC 1 cut(s) 1577
SaqAI TTAA 8 cut(s) 119, 381, 510, 972, 1473, 1941, 2852, 2984
SatI GCNGC 6 cut(s) 144, 183, 1400, 1734, 1749, 2153
Sau96I GGNCC 9 cut(s) 946, 1093, 1094, 1310, 1800, 2371, 2695, 2722, 2963
SchI GAGTC 6 cut(s) 237, 853, 911, 1688, 1795, 3046
ScrFI CCNGG 7 cut(s) 204, 270, 930, 950, 1413, 1583, 2455
SduI GDGCHC 4 cut(s) 496, 1097, 1860, 3013
SfaNI GCATC 7 cut(s) 61, 1042, 1143, 1344, 1720, 2139, 2260
Sfr274I CTCGAG 1 cut(s) 3034
SfuI TTCGAA 1 cut(s) 2912
SinI GGWCC 6 cut(s) 1310, 1800, 2371, 2695, 2722, 2963
SlaI CTCGAG 1 cut(s) 3034
SmiMI CAYNNNNRTG 5 cut(s) 324, 593, 941, 2163, 2256
SmlI CTYRAG 5 cut(s) 893, 971, 1004, 2463, 3034
SmoI CTYRAG 5 cut(s) 893, 971, 1004, 2463, 3034
SphI GCATGC 2 cut(s) 1118, 2612
SseBI AGGCCT 1 cut(s) 378
SsiI CCGC 4 cut(s) 522, 779, 881, 1348
SspI AATATT 4 cut(s) 307, 430, 454, 2016
SspMI CTAG 1 cut(s) 1964
SstI GAGCTC 1 cut(s) 496
StuI AGGCCT 1 cut(s) 378
StyD4I CCNGG 7 cut(s) 202, 268, 928, 948, 1411, 1581, 2453
StyI CCWWGG 4 cut(s) 373, 1518, 1921, 2688
TaaI ACNGT 7 cut(s) 235, 423, 1142, 1196, 1433, 2447, 3001
TaiI ACGT 3 cut(s) 2128, 2898, 3095
TaqI TCGA 8 cut(s) 1081, 1204, 1578, 1648, 1860, 2655, 2912, 3035
TaqII GACCGA 1 cut(s) 236
TatI WGTACW 2 cut(s) 897, 1651
TfiI GAWTC 8 cut(s) 11, 796, 1104, 1253, 2270, 2450, 2468, 2804
Tru1I TTAA 8 cut(s) 119, 381, 510, 972, 1473, 1941, 2852, 2984
Tru9I TTAA 8 cut(s) 119, 381, 510, 972, 1473, 1941, 2852, 2984
TscAI CASTG 5 cut(s) 688, 1201, 2170, 2268, 3060
TseFI GTSAC 1 cut(s) 696
TseI GCWGC 6 cut(s) 143, 182, 1399, 1733, 1748, 2152
Tsp45I GTSAC 1 cut(s) 696
TspGWI ACGGA 1 cut(s) 1116
TspRI CASTG 5 cut(s) 688, 1201, 2170, 2268, 3060
Tth111I GACNNNGTC 1 cut(s) 246
Van91I CCANNNNNTGG 1 cut(s) 2863
Vha464I CTTAAG 1 cut(s) 971
VpaK11BI GGWCC 6 cut(s) 1310, 1800, 2371, 2695, 2722, 2963
XapI RAATTY 4 cut(s) 533, 922, 2821, 2837
XceI RCATGY 5 cut(s) 42, 1118, 2383, 2612, 3072
XcmI CCANNNNNNNNNTGG 2 cut(s) 2491, 2797
XhoI CTCGAG 1 cut(s) 3034
XmiI GTMKAC 2 cut(s) 1578, 2073
XmnI GAANNNNTTC 4 cut(s) 990, 1033, 2397, 2577
XspI CTAG 1 cut(s) 1964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.