MD16G1257100.v1.1

Nuclear pore complex protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004174
Physical Location & Seq
Forward (+)
30130288 .. 30132868
2581 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1257100.v1.1.491

Sequence Viewer

Length: 1713 bp
ATGATGGAAGATAGTGCTTCAGCTCAAGATCTTGTGGAAATGAACCATGGTGAGATTTCTGATGGTGATGAAGAAAGCCAAATGGATCTCACTGGAATTGTGCTTTCGCATTCTCTACCTGTACATCTTAGGCTTGACCCCGTAAAGATGGAAGAAATGAGAATGTTGATGTTCCATGATAAGGAAGAGGAGAGGATGAGTCAAAGATCCACTCCACCAGCTGTGCGAAAAACCCCACTGGCATTTCTAGAGTACAAGCATGGTAGTTTGGACTCAAATTCTCCTGGAACCATTCTAATGGCCCAAGAAAATAAGGCCATGCCCCCTAAGACATTGAAAGAAGGTTTTAAGCTTGATCTCAAGCATGAAACACCAGTAACCAAAAAACATTCTCGCAACATAATTGATACTGGTCTATTTATGGGTAGGTCATTTCGCGTAGGATATGGCCCAAATGGAACCCTTGTCCATGCTGGAACACCATTTGGGAGTAACGGTTCTCCAATGATGTTATCATCCATAATCAATATAGAGAAGGTTGCTATTGACAGTGTTGTTCGAGATGAAAACAACAAAGTTAGGGAGGAACTTGTTGACATGGCTATTGATTCTCCTTTAGATCTTCACAAGGGAATATCTCATCAGACAAATGAGATTGAAGTTGGATCATTCAACTTGAGGCTTCAAAAGCTTGTCTCCAACTGCCTGATGCTTCCACATATTTGTAGGAGCTATGTAGATATTATTGACAAGGAAAATGGTGGAAAATTGAAATCTTTGGGTGCTGATAGTGAGGAAGAAATGGTTCAAGATGTGAATGAAGCTTCTCAACAAGTAGACCTGGAAGCTTTACCTCTTATTTGGAGGGCAGAGTTTAGCTATTGGTTGCAAGAGAATGTTTCCCATCGAGCCTGTGAGTCCATAGTCAATCGTACTGATGTTGCCCAACAACTTGATTGTTGGAGGATCAATAGGCTCGATTACGACTTCATTAAGAAAAACAGGATAAGGCTCTATGAGTTGTTTGCTGGTAATATCCATGGTGCTTTTCATGATGTTAACGTTGATTTGAAGAGGTTTCCAAGGTTGTTTATGTGGTATCATTTAGAACCTAGCACTTCACTGCCTACGATTTTTCACACCTATCAACATCTTCTTGATGATGGTAAAGCTCCATACCCAGTTACAGTCTACATTGATGAAGGACTGGTAAAAGAGGCTGGGAATTCGAATGCGGCAAAATGTTATGATCTATCTTATTATCTGATGCTGCTTCATGCCAGTGAAGAAAGTAAAGTTGGCTTTCTGAAGCCCATGTTTAGTGCCTTCTCGTCAACACACGATCCACTGGATTACCATATGATATGGCATCAGTGTGCAGTGTTGGAAGCAGTTGGTGCTATCAGCTCTAAGGACCTTCATGTTCTTGACATGAGATTTTTTTCCCAACTGTTGTGCCTTGGGCAATGTCATTGGGCCATGTATGTGGTCCTTCACATGCCCCAGTCTGAAGATTTTCCATATGTCCATTCTAATCTTATTTGGGAAATCTTGTTCCAATATTGTGAATCCTGGAGTTCACAAGAATCACAATGCCAAGCGATTGTAAACTTGGGTATTCCTAAGGCATGGCTTCATGAGGCTATGGAACTTAGGAGACTTTCTTTGAGAAGAAAATTTGTTGGCTACCAGTATGCTGTTATTGACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000972 GO:0000973 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003676 GO:0003682 GO:0003712 GO:0003713 GO:0003723 GO:0003729 GO:0005048 GO:0005198 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006403 GO:0006405 GO:0006406 GO:0006606 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0006999 GO:0007154 GO:0007165 GO:0008104 GO:0008139 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009719 GO:0009725 GO:0009733 GO:0009870 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0012505 GO:0015031 GO:0015267 GO:0015288 GO:0015833 GO:0015931 GO:0016020 GO:0016032 GO:0016043 GO:0016234 GO:0016604 GO:0017038 GO:0017056 GO:0019219 GO:0019222 GO:0022607 GO:0022803 GO:0022829 GO:0022857 GO:0023052 GO:0031080 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0031503 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0033036 GO:0033120 GO:0033218 GO:0033365 GO:0034397 GO:0034398 GO:0034399 GO:0034504 GO:0034613 GO:0034622 GO:0034641 GO:0034645 GO:0042221 GO:0042277 GO:0042405 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044614 GO:0044615 GO:0045088 GO:0045089 GO:0045184 GO:0045935 GO:0046483 GO:0046907 GO:0046931 GO:0048024 GO:0048026 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050000 GO:0050657 GO:0050658 GO:0050684 GO:0050685 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051170 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051236 GO:0051252 GO:0051254 GO:0051276 GO:0051292 GO:0051640 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0055085 GO:0060255 GO:0065003 GO:0065007 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0080090 GO:0080134 GO:0090304 GO:0097159 GO:0140110 GO:1901360 GO:1901363 GO:1901576 GO:1902680 GO:1903311 GO:1903313 GO:1903506 GO:1903508 GO:1990841 GO:1990904 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03013 ko05164 map03013 map05164
Pfam Domains
Protein Families
Nucleoporin2 Nup96

Protein Analysis

571

Amino Acids

65.22

Weight (kDa)

5.56

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nup96 PF12110 309 - 551 1.1e-51 Nuclear protein 96
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 837, 1191
AccII CGCG 1 cut(s) 438
AciI CCGC 1 cut(s) 1235
AclI AACGTT 1 cut(s) 1062
AclWI GGATC 5 cut(s) 93, 201, 673, 974, 1337
AcsI RAATTY 3 cut(s) 277, 1225, 1676
AcuI CTGAAG 2 cut(s) 1328, 1530
AfaI GTAC 3 cut(s) 123, 254, 934
AfiI CCNNNNNNNGG 1 cut(s) 181
AgsI TTSAA 7 cut(s) 337, 659, 673, 686, 772, 809, 1072
AjnI CCWGG 3 cut(s) 283, 840, 1571
AjuI GAANNNNNNNTTGG 2 cut(s) 1281, 1313
AloI GAACNNNNNNTCC 4 cut(s) 481, 513, 1538, 1570
Alw26I GTCTC 2 cut(s) 700, 1651
AlwI GGATC 5 cut(s) 93, 201, 673, 974, 1337
AoxI GGCC 4 cut(s) 300, 315, 448, 1476
ApeKI GCWGC 1 cut(s) 1270
ApoI RAATTY 3 cut(s) 277, 1225, 1676
Asp700I GAANNNNTTC 2 cut(s) 804, 1515
AspS9I GGNCC 5 cut(s) 301, 449, 1414, 1476, 1489
AsuHPI GGTGA 2 cut(s) 62, 77
AsuII TTCGAA 1 cut(s) 1229
AvaII GGWCC 2 cut(s) 1414, 1489
AxyI CCTNAGG 1 cut(s) 1623
BbvI GCAGC 1 cut(s) 1257
BccI CCATC 4 cut(s) 56, 142, 912, 1157
BciT130I CCWGG 3 cut(s) 285, 842, 1573
BcoDI GTCTC 2 cut(s) 700, 1651
BfaI CTAG 2 cut(s) 248, 1113
BglII AGATCT 2 cut(s) 28, 619
BisI GCNGC 2 cut(s) 1236, 1271
BlsI GCNGC 2 cut(s) 1237, 1272
Bme1390I CCNGG 3 cut(s) 285, 842, 1573
Bme18I GGWCC 2 cut(s) 1414, 1489
BmgT120I GGNCC 5 cut(s) 301, 449, 1414, 1476, 1489
BmiI GGNNCC 2 cut(s) 289, 460
BmrFI CCNGG 3 cut(s) 285, 842, 1573
BmrI ACTGGG 2 cut(s) 1175, 1498
BmsI GCATC 3 cut(s) 699, 1257, 1378
BmuI ACTGGG 2 cut(s) 1175, 1498
BpmI CTGGAG 1 cut(s) 1594
Bpu14I TTCGAA 1 cut(s) 1229
BpuEI CTTGAG 3 cut(s) 9, 344, 697
BsaJI CCNNGG 4 cut(s) 46, 1039, 1082, 1459
BsaXI ACNNNNNCTCC 2 cut(s) 481, 511
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse21I CCTNAGG 1 cut(s) 1623
Bse3DI GCAATG 1 cut(s) 1472
BseBI CCWGG 3 cut(s) 285, 842, 1573
BseDI CCNNGG 4 cut(s) 46, 1039, 1082, 1459
BseGI GGATG 2 cut(s) 201, 515
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMI GCAATG 1 cut(s) 1472
BseRI GAGGAG 1 cut(s) 203
BseXI GCAGC 1 cut(s) 1257
BseYI CCCAGC 1 cut(s) 1220
BsgI GTGCAG 1 cut(s) 1398
Bsh1236I CGCG 1 cut(s) 438
BshFI GGCC 4 cut(s) 302, 317, 450, 1478
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 2 cut(s) 700, 1651
BsmI GAATGC 2 cut(s) 109, 1237
BsnI GGCC 4 cut(s) 302, 317, 450, 1478
Bsp119I TTCGAA 1 cut(s) 1229
Bsp1407I TGTACA 1 cut(s) 121
Bsp143I GATC 9 cut(s) 28, 85, 206, 355, 619, 665, 966, 1249, 1342
Bsp19I CCATGG 2 cut(s) 46, 1039
BspACI CCGC 1 cut(s) 1235
BspANI GGCC 4 cut(s) 302, 317, 450, 1478
BspFNI CGCG 1 cut(s) 438
BspHI TCATGA 2 cut(s) 1051, 1636
BspLI GGNNCC 2 cut(s) 289, 460
BspPI GGATC 5 cut(s) 93, 201, 673, 974, 1337
BspT104I TTCGAA 1 cut(s) 1229
BsrDI GCAATG 1 cut(s) 1472
BsrGI TGTACA 1 cut(s) 121
BssECI CCNNGG 4 cut(s) 46, 1039, 1082, 1459
BssMI GATC 9 cut(s) 28, 85, 206, 355, 619, 665, 966, 1249, 1342
BssT1I CCWWGG 4 cut(s) 46, 1039, 1082, 1459
Bst2UI CCWGG 3 cut(s) 285, 842, 1573
Bst4CI ACNGT 4 cut(s) 497, 551, 1189, 1452
Bst6I CTCTTC 2 cut(s) 180, 1067
BstAPI GCANNNNNTGC 1 cut(s) 1397
BstAUI TGTACA 1 cut(s) 121
BstBI TTCGAA 1 cut(s) 1229
BstDEI CTNAG 5 cut(s) 128, 327, 1410, 1623, 1652
BstDSI CCRYGG 2 cut(s) 46, 1039
BstF5I GGATG 2 cut(s) 201, 515
BstFNI CGCG 1 cut(s) 438
BstKTI GATC 9 cut(s) 31, 88, 209, 358, 622, 668, 969, 1252, 1345
BstMAI GTCTC 2 cut(s) 700, 1651
BstMBI GATC 9 cut(s) 28, 85, 206, 355, 619, 665, 966, 1249, 1342
BstMWI GCNNNNNNNGC 2 cut(s) 688, 1397
BstNI CCWGG 3 cut(s) 285, 842, 1573
BstNSI RCATGY 1 cut(s) 1501
BstSCI CCNGG 3 cut(s) 283, 840, 1571
BstUI CGCG 1 cut(s) 438
BstV1I GCAGC 1 cut(s) 1257
BstX2I RGATCY 4 cut(s) 28, 85, 206, 619
BstXI CCANNNNNNTGG 2 cut(s) 298, 1486
BstYI RGATCY 4 cut(s) 28, 85, 206, 619
Bsu36I CCTNAGG 1 cut(s) 1623
BsuRI GGCC 4 cut(s) 302, 317, 450, 1478
BtgI CCRYGG 2 cut(s) 46, 1039
BtsCI GGATG 2 cut(s) 201, 515
BtsI GCAGTG 2 cut(s) 1121, 1386
BtsIMutI CAGTG 8 cut(s) 90, 236, 556, 1121, 1288, 1346, 1379, 1386
CciI TCATGA 2 cut(s) 1051, 1636
Cfr13I GGNCC 5 cut(s) 301, 449, 1414, 1476, 1489
Csp6I GTAC 3 cut(s) 122, 253, 933
CviQI GTAC 3 cut(s) 122, 253, 933
DdeI CTNAG 5 cut(s) 128, 327, 1410, 1623, 1652
DpnI GATC 9 cut(s) 30, 87, 208, 357, 621, 667, 968, 1251, 1344
DpnII GATC 9 cut(s) 28, 85, 206, 355, 619, 665, 966, 1249, 1342
Eam1104I CTCTTC 2 cut(s) 180, 1067
EarI CTCTTC 2 cut(s) 180, 1067
Eco130I CCWWGG 4 cut(s) 46, 1039, 1082, 1459
Eco47I GGWCC 2 cut(s) 1414, 1489
Eco57I CTGAAG 2 cut(s) 1328, 1530
Eco81I CCTNAGG 1 cut(s) 1623
EcoO109I RGGNCCY 1 cut(s) 1414
EcoRI GAATTC 1 cut(s) 1225
EcoRII CCWGG 3 cut(s) 283, 840, 1571
EcoT14I CCWWGG 4 cut(s) 46, 1039, 1082, 1459
ErhI CCWWGG 4 cut(s) 46, 1039, 1082, 1459
FauNDI CATATG 2 cut(s) 1359, 1522
FblI GTMKAC 2 cut(s) 837, 1191
Fnu4HI GCNGC 2 cut(s) 1236, 1271
FokI GGATG 2 cut(s) 208, 502
Fsp4HI GCNGC 2 cut(s) 1236, 1271
FspBI CTAG 2 cut(s) 248, 1113
GluI GCNGC 2 cut(s) 1236, 1271
GsaI CCCAGC 1 cut(s) 1224
GsuI CTGGAG 1 cut(s) 1594
HaeIII GGCC 4 cut(s) 302, 317, 450, 1478
HincII GTYRAC 3 cut(s) 595, 1060, 1335
HindII GTYRAC 3 cut(s) 595, 1060, 1335
HindIII AAGCTT 4 cut(s) 350, 689, 822, 846
HinfI GANTC 6 cut(s) 199, 272, 608, 917, 1568, 1586
HpaI GTTAAC 1 cut(s) 1060
HphI GGTGA 2 cut(s) 62, 77
Hpy166II GTNNAC 7 cut(s) 595, 838, 1060, 1192, 1335, 1580, 1609
Hpy188I TCNGA 5 cut(s) 61, 645, 1266, 1308, 1510
Hpy188III TCNNGA 8 cut(s) 26, 248, 560, 809, 1052, 1157, 1427, 1637
Hpy8I GTNNAC 7 cut(s) 595, 838, 1060, 1192, 1335, 1580, 1609
HpyAV CCTTC 6 cut(s) 335, 529, 1196, 1336, 1427, 1502
HpyCH4III ACNGT 4 cut(s) 497, 551, 1189, 1452
HpyCH4IV ACGT 1 cut(s) 1062
HpyCH4V TGCA 2 cut(s) 889, 1379
HpyF10VI GCNNNNNNNGC 2 cut(s) 688, 1397
HpyF3I CTNAG 5 cut(s) 128, 327, 1410, 1623, 1652
HpySE526I ACGT 1 cut(s) 1062
KspAI GTTAAC 1 cut(s) 1060
Kzo9I GATC 9 cut(s) 28, 85, 206, 355, 619, 665, 966, 1249, 1342
LmnI GCTCC 2 cut(s) 729, 1177
Lsp1109I GCAGC 1 cut(s) 1257
LweI GCATC 3 cut(s) 699, 1257, 1378
MaeI CTAG 2 cut(s) 248, 1113
MaeII ACGT 1 cut(s) 1062
MaeIII GTNAC 3 cut(s) 376, 491, 1183
MalI GATC 9 cut(s) 30, 87, 208, 357, 621, 667, 968, 1251, 1344
MboI GATC 9 cut(s) 28, 85, 206, 355, 619, 665, 966, 1249, 1342
MflI RGATCY 4 cut(s) 28, 85, 206, 619
MluCI AATT 6 cut(s) 96, 277, 402, 767, 1225, 1676
MlyI GAGTC 3 cut(s) 208, 266, 926
MmeI TCCRAC 4 cut(s) 643, 723, 941, 1365
MroXI GAANNNNTTC 2 cut(s) 804, 1515
MseI TTAA 4 cut(s) 348, 993, 1059, 1711
MslI CAYNNNNRTG 4 cut(s) 296, 1281, 1374, 1484
MspA1I CMGCKG 1 cut(s) 221
MspR9I CCNGG 3 cut(s) 285, 842, 1573
Mva1269I GAATGC 2 cut(s) 109, 1237
MvaI CCWGG 3 cut(s) 285, 842, 1573
MvnI CGCG 1 cut(s) 438
MwoI GCNNNNNNNGC 2 cut(s) 688, 1397
NcoI CCATGG 2 cut(s) 46, 1039
NdeI CATATG 2 cut(s) 1359, 1522
NdeII GATC 9 cut(s) 28, 85, 206, 355, 619, 665, 966, 1249, 1342
NlaIV GGNNCC 2 cut(s) 289, 460
NspI RCATGY 1 cut(s) 1501
NspV TTCGAA 1 cut(s) 1229
PagI TCATGA 2 cut(s) 1051, 1636
PctI GAATGC 2 cut(s) 109, 1237
PdmI GAANNNNTTC 2 cut(s) 804, 1515
PfeI GAWTC 3 cut(s) 608, 1568, 1586
PfoI TCCNGGA 2 cut(s) 283, 1571
PkrI GCNGC 2 cut(s) 1237, 1272
PleI GAGTC 3 cut(s) 207, 266, 925
PpsI GAGTC 3 cut(s) 207, 266, 925
PpuMI RGGWCCY 1 cut(s) 1414
Psp1406I AACGTT 1 cut(s) 1062
Psp5II RGGWCCY 1 cut(s) 1414
Psp6I CCWGG 3 cut(s) 283, 840, 1571
PspFI CCCAGC 1 cut(s) 1220
PspGI CCWGG 3 cut(s) 283, 840, 1571
PspN4I GGNNCC 2 cut(s) 289, 460
PspPI GGNCC 5 cut(s) 301, 449, 1414, 1476, 1489
PspPPI RGGWCCY 1 cut(s) 1414
PsuI RGATCY 4 cut(s) 28, 85, 206, 619
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 3 cut(s) 123, 254, 934
RsaNI GTAC 3 cut(s) 122, 253, 933
RseI CAYNNNNRTG 4 cut(s) 296, 1281, 1374, 1484
SaqAI TTAA 4 cut(s) 348, 993, 1059, 1711
SatI GCNGC 2 cut(s) 1236, 1271
Sau3AI GATC 9 cut(s) 28, 85, 206, 355, 619, 665, 966, 1249, 1342
Sau96I GGNCC 5 cut(s) 301, 449, 1414, 1476, 1489
SchI GAGTC 3 cut(s) 208, 266, 926
ScrFI CCNGG 3 cut(s) 285, 842, 1573
SfaNI GCATC 3 cut(s) 699, 1257, 1378
SfuI TTCGAA 1 cut(s) 1229
SinI GGWCC 2 cut(s) 1414, 1489
SmiMI CAYNNNNRTG 4 cut(s) 296, 1281, 1374, 1484
SmlI CTYRAG 3 cut(s) 24, 359, 676
SmoI CTYRAG 3 cut(s) 24, 359, 676
Sse9I AATT 6 cut(s) 96, 277, 402, 767, 1225, 1676
SsiI CCGC 1 cut(s) 1235
SspI AATATT 1 cut(s) 1562
SspMI CTAG 2 cut(s) 248, 1113
StyD4I CCNGG 3 cut(s) 283, 840, 1571
StyI CCWWGG 4 cut(s) 46, 1039, 1082, 1459
TaaI ACNGT 4 cut(s) 497, 551, 1189, 1452
TaiI ACGT 1 cut(s) 1065
TaqI TCGA 4 cut(s) 559, 907, 978, 1229
TasI AATT 6 cut(s) 96, 277, 402, 767, 1225, 1676
TatI WGTACW 2 cut(s) 121, 252
TauI GCSGC 1 cut(s) 1238
TfiI GAWTC 3 cut(s) 608, 1568, 1586
Tru1I TTAA 4 cut(s) 348, 993, 1059, 1711
Tru9I TTAA 4 cut(s) 348, 993, 1059, 1711
TscAI CASTG 8 cut(s) 97, 243, 556, 1128, 1288, 1353, 1379, 1386
TseI GCWGC 1 cut(s) 1270
TspRI CASTG 8 cut(s) 97, 243, 556, 1128, 1288, 1353, 1379, 1386
VpaK11BI GGWCC 2 cut(s) 1414, 1489
XapI RAATTY 3 cut(s) 277, 1225, 1676
XbaI TCTAGA 1 cut(s) 247
XceI RCATGY 1 cut(s) 1501
XmiI GTMKAC 2 cut(s) 837, 1191
XmnI GAANNNNTTC 2 cut(s) 804, 1515
XspI CTAG 2 cut(s) 248, 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.