Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006826

Physical Location & Seq

Genomic Coordinates Forward (+)

28300406 .. 28305240

Gene Structure 4835 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1220200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1356 bp

ATGAAGGTGAAAGTAATATCTCGATCCGCAGATGAATTCACTAAAGAACGAAGCCAAGACCTCCAGAGGGTATTCCGTAACTTCGACCCCAGCATCCGAACCCAAGAGAAAGCCGTCGAGTATGTCCGGGCACAAAACGCTGCAAAATTAGATAAGATATTCGCGAGGCCGTTTGTTGGAGCAATGGAGGGACATATAGATTCCATTTCCTGCATGGCAAAGAATCCAAACCACTTGATAGGAATATTTTCTGGTTCAATGGATGGAGAAATTCGGCTTTGGGATCTAGCTTCCAGGCGAACAGTTTGTCGGTTTCCTGGTCATAAAGGTGCTGTCCAAGGTTTAACAGTATCTACAGATGGGCGTGTTCTTGTGTCTTGTGGAACTGATAGCACTGTTAGGCTTTGGAGTGTACCTGTTTCTAATCTCCTGGAGGAAGATGCCTCATCCGATAAGTCTGCTCAGCCACGGGAAACTTATGTTTGGAAGAATGCGTTCCGGTCTGTTGATCACCAGTGGAATTCTGATCGGTTTGCTACGGGGGGAGCTGGAGTAGATATATGGAACCACAACAGGTCTGCACCAGAGCAGAGTTACCAATGGGGCACAGACACAGTTGTATCTGTTCGATTTAATCCTGGGGAGCCCGATATCTTGGCAACATCTGCAAGTGACCGCAGCATAGCACTATATGACTTGCGCATGGGAAAACCAACATCAAAGTTTATAATGAGGACAAGAACCAATTCCATTTGTTGGAACCCAATGGAGCCAATCAACTTCACTGCTGCAAACGAAGACTCCAACTGCTATAGCTTTGATTGTAGAAAGTTTGGTGAAGCTACGTGTGTTCACATGGATCATGTTTCTGCTGTGATGGATATTGATTATTCTCCAACTGGTCGAGAATTTGTCACTGGGTCTTATGATAGAACTATCATGATTTTCCCGTATAATGCTGGTCACAAACGGGAGATTTATCATACGAAGAGAATGCAAAGGGTGTTTTGTGTCAAGTACAGCTGCGATGGTAGTTATGTTATTTCTGGGAGTGATGATACTAATCTTAGGCTTTGGAAGTCTAAAGCATCAGAACAATTGGGAGTTCTTCTTCCAAGGGAACAGAAAAAGCACGAGTATCATGAGGCGGTCAAAAACCGCTACAAGCACCTTCCTGAAATCAAGCGTATTGTGAGGCATAGGCACCTACCGAAACCAATATACAAGGCAGGTAAGCAAATTCGTGAACAAAATGAATCTCAGAGAAAGAAGCAAGAGAAGAGGAAGGCGCACAGTGCCCCAGGAAGCATAGTTGAAGAGCCATTACGGAAAAGAAAAATCATCAAAGTCGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000462 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030684 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:1901360 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sof1 WD40

Protein Analysis

452

Amino Acids

51.57

Weight (kDa)

9.45

Isoelectric Point (pI)

44.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	59 - 145	9.3e-16	WDR5 beta-propeller domain
WD40	PF00400	59 - 95	1e-05	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	59 - 195	6.6e-14	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	61 - 141	3e-16	WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	61 - 145	3.4e-07	CAF1B/HIR1 beta-propeller domain
WDR55	PF24796	61 - 138	5.1e-10	WDR55
Beta-prop_THOC3	PF25174	62 - 144	3.6e-10	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	63 - 137	2.4e-06	WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_2nd	PF25172	63 - 137	1.5e-10	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	63 - 139	1.2e-06	G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd	PF25047	65 - 139	2.2e-08	TEP-1 second beta-propeller
WD40_Prp19	PF24814	68 - 213	3.6e-12	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	71 - 258	3.2e-10	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	74 - 144	2.1e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_DCAF4	PF23761	83 - 149	2.7e-07	DDB1- and CUL4-associated factor 4 beta-propeller domain
WD40	PF00400	102 - 137	8.6e-08	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	165 - 362	1.3e-24	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	185 - 318	1.3e-10	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	203 - 376	8.1e-19	WDR5 beta-propeller domain
WD40_Prp19	PF24814	206 - 367	1.9e-12	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	211 - 367	2.5e-13	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	220 - 372	1.2e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st	PF25173	222 - 365	3.1e-11	WDR3 first beta-propeller domain
WDR55	PF24796	246 - 364	1.6e-07	WDR55
WD40_WDHD1_1st	PF24817	262 - 367	9.4e-06	WDHD1 first WD40 domain
Sof1	PF04158	361 - 447	7.6e-28	Sof1-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006826)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28450
fragaria_vesca	FvH4_2g10410 FvH4_2g10410
malus_domestica	MD08G1220200.v1.1 MD15G1410600.v1.1 MD15G1410700.v1.1
prunus_persica	Prupe.1G573700_v2.0.a1 Prupe.1G573700_v2.0.a1 Prupe.3G166600_v2.0.a1 Prupe.6G177000_v2.0.a1 Prupe.6G177000_v2.0.a1 Prupe.6G177000_v2.0.a1 Prupe.6G177000_v2.0.a1
pyrus_communis	pycom08g19110 pycom15g36550
rosa_chinensis	RchiOBHm_Chr6g0267881
rosa_laevigata	RLG00000013973
rosa_multiflora	Rmu_co8335391.1_g000001 Rmu_sc0000434.1_g000004 Rmu_sc0005400.1_g000008
rosa_roxburghii	Rroxscaffold_7G00200360
rosa_rugosa	Rorug06G0034600
rosa_samantha	Rh6AG156600 Rh6BG157800 Rh6DG143000
rosa_wichuraiana	Rw6G013600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	728
Acc16I	TGCGCA	1 cut(s)	701
Acc36I	ACCTGC	1 cut(s)	1220
AccB1I	GGYRCC	1 cut(s)	1203
AccB7I	CCANNNNNTGG	1 cut(s)	756
AccII	CGCG	1 cut(s)	164
AciI	CCGC	4 cut(s)	27, 676, 1148, 1159
AclWI	GGATC	3 cut(s)	18, 291, 867
AcsI	RAATTY	5 cut(s)	35, 270, 520, 908, 1239
AfaI	GTAC	2 cut(s)	414, 1019
AfiI	CCNNNNNNNGG	3 cut(s)	67, 176, 756
AflIII	ACRYGT	1 cut(s)	845
AgsI	TTSAA	2 cut(s)	258, 1316
AjnI	CCWGG	5 cut(s)	293, 316, 429, 637, 1300
AluBI	AGCT	5 cut(s)	290, 548, 816, 842, 1023
AluI	AGCT	5 cut(s)	290, 548, 816, 842, 1023
AlwI	GGATC	3 cut(s)	18, 291, 867
AoxI	GGCC	1 cut(s)	167
ApeKI	GCWGC	4 cut(s)	140, 678, 788, 1023
ApoI	RAATTY	5 cut(s)	35, 270, 520, 908, 1239
ArsI	GACNNNNNNTTYG	2 cut(s)	292, 324
Asp700I	GAANNNNTTC	3 cut(s)	247, 494, 745
AspLEI	GCGC	2 cut(s)	702, 1291
AsuC2I	CCSGG	1 cut(s)	128
AsuHPI	GGTGA	3 cut(s)	19, 503, 848
BaeGI	GKGCMC	3 cut(s)	133, 608, 1300
BaeI	ACNNNNGTAYC	2 cut(s)	603, 636
BanI	GGYRCC	1 cut(s)	1203
BanII	GRGCYC	1 cut(s)	648
BarI	GAAGNNNNNNTAC	2 cut(s)	1308, 1340
BauI	CACGAG	1 cut(s)	1133
BbsI	GAAGAC	1 cut(s)	804
BbvI	GCAGC	4 cut(s)	127, 690, 775, 1010
BccI	CCATC	4 cut(s)	257, 353, 871, 1022
BceAI	ACGGC	2 cut(s)	98, 154
BcgI	CGANNNNNNTGC	4 cut(s)	440, 474, 976, 1010
BciT130I	CCWGG	5 cut(s)	295, 318, 431, 639, 1302
BclI	TGATCA	1 cut(s)	508
BcnI	CCSGG	1 cut(s)	128
BfaI	CTAG	1 cut(s)	287
BfmI	CTRYAG	2 cut(s)	354, 811
BfuAI	ACCTGC	1 cut(s)	1220
BisI	GCNGC	4 cut(s)	141, 679, 789, 1024
BlpI	GCTNAGC	1 cut(s)	462
BlsI	GCNGC	4 cut(s)	142, 680, 790, 1025
Bme1390I	CCNGG	6 cut(s)	128, 295, 318, 431, 639, 1302
BmiI	GGNNCC	5 cut(s)	566, 645, 761, 771, 1205
BmrFI	CCNGG	6 cut(s)	128, 295, 318, 431, 639, 1302
BmrI	ACTGGG	1 cut(s)	927
BmsI	GCATC	3 cut(s)	102, 430, 1097
BmuI	ACTGGG	1 cut(s)	927
BpiI	GAAGAC	1 cut(s)	804
BpmI	CTGGAG	3 cut(s)	47, 452, 570
Bpu1102I	GCTNAGC	1 cut(s)	462
BpuMI	CCSGG	1 cut(s)	128
BsaAI	YACGTR	1 cut(s)	846
BsaBI	GATNNNNATC	1 cut(s)	1062
BsaJI	CCNNGG	5 cut(s)	337, 467, 638, 1115, 1300
BsaWI	WCCGGW	1 cut(s)	498
BsaXI	ACNNNNNCTCC	4 cut(s)	537, 567, 785, 815
Bsc4I	CCNNNNNNNGG	3 cut(s)	67, 176, 756
Bse1I	ACTGG	3 cut(s)	514, 904, 922
Bse3DI	GCAATG	1 cut(s)	189
Bse8I	GATNNNNATC	1 cut(s)	1062
BseBI	CCWGG	5 cut(s)	295, 318, 431, 639, 1302
BseDI	CCNNGG	5 cut(s)	337, 467, 638, 1115, 1300
BseGI	GGATG	3 cut(s)	93, 268, 446
BseJI	GATNNNNATC	1 cut(s)	1062
BseLI	CCNNNNNNNGG	3 cut(s)	67, 176, 756
BseMI	GCAATG	1 cut(s)	189
BseMII	CTCAG	2 cut(s)	476, 1274
BseNI	ACTGG	3 cut(s)	514, 904, 922
BseSI	GKGCMC	3 cut(s)	133, 608, 1300
BseXI	GCAGC	4 cut(s)	127, 690, 775, 1010
BseYI	CCCAGC	1 cut(s)	89
BsgI	GTGCAG	1 cut(s)	564
Bsh1236I	CGCG	1 cut(s)	164
BshFI	GGCC	1 cut(s)	169
BshNI	GGYRCC	1 cut(s)	1203
BsiSI	CCGG	2 cut(s)	127, 499
BslFI	GGGAC	1 cut(s)	204
BslI	CCNNNNNNNGG	3 cut(s)	67, 176, 756
BsmFI	GGGAC	1 cut(s)	204
BsmI	GAATGC	2 cut(s)	496, 999
BsnI	GGCC	1 cut(s)	169
Bsp1286I	GDGCHC	4 cut(s)	133, 608, 648, 1300
Bsp143I	GATC	5 cut(s)	23, 283, 508, 526, 859
Bsp1720I	GCTNAGC	1 cut(s)	462
Bsp68I	TCGCGA	1 cut(s)	164
BspACI	CCGC	4 cut(s)	27, 676, 1148, 1159
BspANI	GGCC	1 cut(s)	169
BspCNI	CTCAG	2 cut(s)	475, 1273
BspFNI	CGCG	1 cut(s)	164
BspHI	TCATGA	2 cut(s)	939, 1141
BspLI	GGNNCC	5 cut(s)	566, 645, 761, 771, 1205
BspMI	ACCTGC	1 cut(s)	1220
BspPI	GGATC	3 cut(s)	18, 291, 867
BspQI	GCTCTTC	1 cut(s)	1311
BspT107I	GGYRCC	1 cut(s)	1203
BsrDI	GCAATG	1 cut(s)	189
BsrI	ACTGG	3 cut(s)	514, 904, 922
BssECI	CCNNGG	5 cut(s)	337, 467, 638, 1115, 1300
BssMI	GATC	5 cut(s)	23, 283, 508, 526, 859
BssSI	CACGAG	1 cut(s)	1133
BssT1I	CCWWGG	2 cut(s)	337, 1115
Bst2BI	CACGAG	1 cut(s)	1133
Bst2UI	CCWGG	5 cut(s)	295, 318, 431, 639, 1302
Bst4CI	ACNGT	5 cut(s)	304, 349, 397, 616, 1295
Bst6I	CTCTTC	3 cut(s)	983, 1274, 1311
BstAPI	GCANNNNNTGC	1 cut(s)	665
BstBAI	YACGTR	1 cut(s)	846
BstDEI	CTNAG	3 cut(s)	462, 1067, 1260
BstDSI	CCRYGG	1 cut(s)	467
BstF5I	GGATG	3 cut(s)	93, 268, 446
BstFNI	CGCG	1 cut(s)	164
BstHHI	GCGC	2 cut(s)	702, 1291
BstKTI	GATC	5 cut(s)	26, 286, 511, 529, 862
BstMBI	GATC	5 cut(s)	23, 283, 508, 526, 859
BstMWI	GCNNNNNNNGC	3 cut(s)	137, 665, 1295
BstNI	CCWGG	5 cut(s)	295, 318, 431, 639, 1302
BstSCI	CCNGG	6 cut(s)	126, 293, 316, 429, 637, 1300
BstSFI	CTRYAG	2 cut(s)	354, 811
BstSLI	GKGCMC	3 cut(s)	133, 608, 1300
BstUI	CGCG	1 cut(s)	164
BstV1I	GCAGC	4 cut(s)	127, 690, 775, 1010
BstV2I	GAAGAC	1 cut(s)	804
BstX2I	RGATCY	1 cut(s)	283
BstYI	RGATCY	1 cut(s)	283
BsuRI	GGCC	1 cut(s)	169
BtgI	CCRYGG	1 cut(s)	467
BtgZI	GCGATG	1 cut(s)	1041
BtsCI	GGATG	3 cut(s)	93, 268, 446
BtsI	GCAGTG	1 cut(s)	783
BtsIMutI	CAGTG	5 cut(s)	393, 521, 783, 915, 1300
BtuMI	TCGCGA	1 cut(s)	164
BveI	ACCTGC	1 cut(s)	1220
CciI	TCATGA	2 cut(s)	939, 1141
CfoI	GCGC	2 cut(s)	702, 1291
Csp6I	GTAC	2 cut(s)	413, 1018
CviAII	CATG	6 cut(s)	214, 703, 856, 863, 940, 1142
CviQI	GTAC	2 cut(s)	413, 1018
DdeI	CTNAG	3 cut(s)	462, 1067, 1260
DpnI	GATC	5 cut(s)	25, 285, 510, 528, 861
DpnII	GATC	5 cut(s)	23, 283, 508, 526, 859
Eam1104I	CTCTTC	3 cut(s)	983, 1274, 1311
EarI	CTCTTC	3 cut(s)	983, 1274, 1311
Eco130I	CCWWGG	2 cut(s)	337, 1115
Eco24I	GRGCYC	1 cut(s)	648
Eco32I	GATATC	1 cut(s)	652
EcoRI	GAATTC	2 cut(s)	35, 520
EcoRII	CCWGG	5 cut(s)	293, 316, 429, 637, 1300
EcoRV	GATATC	1 cut(s)	652
EcoT14I	CCWWGG	2 cut(s)	337, 1115
EcoT38I	GRGCYC	1 cut(s)	648
ErhI	CCWWGG	2 cut(s)	337, 1115
FaeI	CATG	6 cut(s)	217, 706, 859, 866, 943, 1145
FaqI	GGGAC	1 cut(s)	204
FatI	CATG	6 cut(s)	213, 702, 855, 862, 939, 1141
FbaI	TGATCA	1 cut(s)	508
Fnu4HI	GCNGC	4 cut(s)	141, 679, 789, 1024
FokI	GGATG	3 cut(s)	80, 275, 433
FriOI	GRGCYC	1 cut(s)	648
Fsp4HI	GCNGC	4 cut(s)	141, 679, 789, 1024
FspBI	CTAG	1 cut(s)	287
FspI	TGCGCA	1 cut(s)	701
GlaI	GCGC	2 cut(s)	701, 1290
GluI	GCNGC	4 cut(s)	141, 679, 789, 1024
GsaI	CCCAGC	1 cut(s)	93
GsuI	CTGGAG	3 cut(s)	47, 452, 570
HaeIII	GGCC	1 cut(s)	169
HapII	CCGG	2 cut(s)	127, 499
HhaI	GCGC	2 cut(s)	702, 1291
Hin1II	CATG	6 cut(s)	217, 706, 859, 866, 943, 1145
Hin6I	GCGC	2 cut(s)	700, 1289
HinP1I	GCGC	2 cut(s)	700, 1289
HinfI	GANTC	4 cut(s)	200, 223, 800, 1256
HpaII	CCGG	2 cut(s)	127, 499
HphI	GGTGA	3 cut(s)	19, 503, 848
Hpy166II	GTNNAC	3 cut(s)	413, 853, 1247
Hpy188I	TCNGA	5 cut(s)	98, 451, 526, 1093, 1263
Hpy188III	TCNNGA	8 cut(s)	21, 64, 163, 905, 940, 1142, 1175, 1244
Hpy8I	GTNNAC	3 cut(s)	413, 853, 1247
Hpy99I	CGWCG	1 cut(s)	119
HpyAV	CCTTC	2 cut(s)	1181, 1279
HpyCH4III	ACNGT	5 cut(s)	304, 349, 397, 616, 1295
HpyCH4IV	ACGT	1 cut(s)	845
HpyCH4V	TGCA	6 cut(s)	143, 213, 581, 668, 791, 997
HpyF10VI	GCNNNNNNNGC	3 cut(s)	137, 665, 1295
HpyF3I	CTNAG	3 cut(s)	462, 1067, 1260
HpySE526I	ACGT	1 cut(s)	845
Hsp92II	CATG	6 cut(s)	217, 706, 859, 866, 943, 1145
HspAI	GCGC	2 cut(s)	700, 1289
Ksp22I	TGATCA	1 cut(s)	508
Kzo9I	GATC	5 cut(s)	23, 283, 508, 526, 859
LguI	GCTCTTC	1 cut(s)	1311
LmnI	GCTCC	4 cut(s)	179, 545, 643, 769
Lsp1109I	GCAGC	4 cut(s)	127, 690, 775, 1010
LweI	GCATC	3 cut(s)	102, 430, 1097
MaeI	CTAG	1 cut(s)	287
MaeII	ACGT	1 cut(s)	845
MaeIII	GTNAC	5 cut(s)	77, 593, 671, 913, 962
MalI	GATC	5 cut(s)	25, 285, 510, 528, 861
MboI	GATC	5 cut(s)	23, 283, 508, 526, 859
MboII	GAAGA	8 cut(s)	449, 499, 809, 1000, 1100, 1103, 1291, 1328
MfeI	CAATTG	1 cut(s)	1097
MflI	RGATCY	1 cut(s)	283
MhlI	GDGCHC	4 cut(s)	133, 608, 648, 1300
MluCI	AATT	8 cut(s)	35, 146, 270, 520, 745, 908, 1097, 1239
MlyI	GAGTC	1 cut(s)	794
MmeI	TCCRAC	4 cut(s)	157, 737, 828, 920
MroXI	GAANNNNTTC	3 cut(s)	247, 494, 745
MseI	TTAA	2 cut(s)	344, 633
MslI	CAYNNNNRTG	1 cut(s)	327
MspA1I	CMGCKG	1 cut(s)	1023
MspI	CCGG	2 cut(s)	127, 499
MspR9I	CCNGG	6 cut(s)	128, 295, 318, 431, 639, 1302
MunI	CAATTG	1 cut(s)	1097
Mva1269I	GAATGC	2 cut(s)	496, 999
MvaI	CCWGG	5 cut(s)	295, 318, 431, 639, 1302
MvnI	CGCG	1 cut(s)	164
MwoI	GCNNNNNNNGC	3 cut(s)	137, 665, 1295
NciI	CCSGG	1 cut(s)	128
NdeII	GATC	5 cut(s)	23, 283, 508, 526, 859
NlaIII	CATG	6 cut(s)	217, 706, 859, 866, 943, 1145
NlaIV	GGNNCC	5 cut(s)	566, 645, 761, 771, 1205
NmuCI	GTSAC	3 cut(s)	671, 913, 962
NruI	TCGCGA	1 cut(s)	164
NsbI	TGCGCA	1 cut(s)	701
PagI	TCATGA	2 cut(s)	939, 1141
PciSI	GCTCTTC	1 cut(s)	1311
PctI	GAATGC	2 cut(s)	496, 999
PdmI	GAANNNNTTC	3 cut(s)	247, 494, 745
PfeI	GAWTC	3 cut(s)	200, 223, 1256
PflMI	CCANNNNNTGG	1 cut(s)	756
PfoI	TCCNGGA	1 cut(s)	429
PkrI	GCNGC	4 cut(s)	142, 680, 790, 1025
PleI	GAGTC	1 cut(s)	794
PpsI	GAGTC	1 cut(s)	794
Ppu21I	YACGTR	1 cut(s)	846
PsiI	TTATAA	1 cut(s)	728
Psp6I	CCWGG	5 cut(s)	293, 316, 429, 637, 1300
PspFI	CCCAGC	1 cut(s)	89
PspGI	CCWGG	5 cut(s)	293, 316, 429, 637, 1300
PspN4I	GGNNCC	5 cut(s)	566, 645, 761, 771, 1205
PsuI	RGATCY	1 cut(s)	283
PvuII	CAGCTG	1 cut(s)	1023
RruI	TCGCGA	1 cut(s)	164
RsaI	GTAC	2 cut(s)	414, 1019
RsaNI	GTAC	2 cut(s)	413, 1018
RseI	CAYNNNNRTG	1 cut(s)	327
SapI	GCTCTTC	1 cut(s)	1311
SaqAI	TTAA	2 cut(s)	344, 633
SatI	GCNGC	4 cut(s)	141, 679, 789, 1024
Sau3AI	GATC	5 cut(s)	23, 283, 508, 526, 859
SchI	GAGTC	1 cut(s)	794
ScrFI	CCNGG	6 cut(s)	128, 295, 318, 431, 639, 1302
SduI	GDGCHC	4 cut(s)	133, 608, 648, 1300
SfaNI	GCATC	3 cut(s)	102, 430, 1097
SfcI	CTRYAG	2 cut(s)	354, 811
SmiMI	CAYNNNNRTG	1 cut(s)	327
Sse9I	AATT	8 cut(s)	35, 146, 270, 520, 745, 908, 1097, 1239
SsiI	CCGC	4 cut(s)	27, 676, 1148, 1159
SspI	AATATT	1 cut(s)	246
SspMI	CTAG	1 cut(s)	287
StyD4I	CCNGG	6 cut(s)	126, 293, 316, 429, 637, 1300
StyI	CCWWGG	2 cut(s)	337, 1115
TaaI	ACNGT	5 cut(s)	304, 349, 397, 616, 1295
TaiI	ACGT	1 cut(s)	848
TaqI	TCGA	6 cut(s)	22, 84, 117, 628, 904, 1350
TasI	AATT	8 cut(s)	35, 146, 270, 520, 745, 908, 1097, 1239
TatI	WGTACW	1 cut(s)	1017
TfiI	GAWTC	3 cut(s)	200, 223, 1256
Tru1I	TTAA	2 cut(s)	344, 633
Tru9I	TTAA	2 cut(s)	344, 633
TscAI	CASTG	5 cut(s)	400, 521, 790, 922, 1300
TseFI	GTSAC	3 cut(s)	671, 913, 962
TseI	GCWGC	4 cut(s)	140, 678, 788, 1023
Tsp45I	GTSAC	3 cut(s)	671, 913, 962
TspDTI	ATGAA	3 cut(s)	17, 48, 1269
TspGWI	ACGGA	2 cut(s)	65, 1342
TspRI	CASTG	5 cut(s)	400, 521, 790, 922, 1300
Van91I	CCANNNNNTGG	1 cut(s)	756
XapI	RAATTY	5 cut(s)	35, 270, 520, 908, 1239
XcmI	CCANNNNNNNNNTGG	1 cut(s)	211
XmnI	GAANNNNTTC	3 cut(s)	247, 494, 745
XspI	CTAG	1 cut(s)	287

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.