Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0006826

Physical Location & Seq

Genomic Coordinates Forward (+)

24367692 .. 24373566

Gene Structure 5875 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG156600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1134 bp

ATGAACCACTTGGTAGGAATATTCTCCGGTTCAATGGATGGAGATATTCGGCATTGGGATATAGCTTCCAGGCGCACAGTTCATCAGTTTCCTGGCCACCAAGGTGCTGTGCGAGGTTTAGCAGTCTCTACAGATGGACGTGTTCTTGTCTCTTGTGGAACTGATTGCAGTGTCAGGCTCTGGGCCGTACCTATTTCTACTGCGATGGAGCTAGATGTCTCATCTGACAAGTCTGTTGAGCCGCTAGAAACTTATGTTTGGAAGAATGCATTTCGGTCTGTTGATCACCAATGGAATTTTGATCGCTTTGCCACAGGTGGAGCTGGTGTAGACATATGGAACCACAACAGGTCTGCACCGGAACAGAGTTTGGAATGGGGAACAGACACAGTTATATCTGTCCGGTTTAATCCTGGACAGCCTGATCTTTTGGCAACATCCGCAAGTGATCGCAGCATAGCACTATATGACTTACGCATGGGGAAACCAACAGCGAAGCTTATCATGGCGACAAAAACCAATTCTATTTGTTGGAACCCAATGGAGCCATTCAACTTCACTGCTGCAAATGAGGACTGCAATTGCTATAGCTTTGATTCTAGAAAGCTTAATGCAGCAACGTGTGTGCACAAAGATCATGTTTCTGCTGTGATGGATATAGATTATTCTCCAACTGGTAGAGAATTTGTAACTGGGTCTTATGATAGAACAGTGAGAATTTTCCAGTATAATGGTGGTCACAGCCGGGAGATTTATCATACAAAGAGAATGCAAAGGGTGTTTTGCGTCAAGTTCAGCTGTGATGCTAGTTATGTTATTTCTGGGAGTGATGACACGAATCTTAGGCTTTGGAAGGCTAAAGCATCAGAACAGTTGGGAGTTCTTCTTCCAAGGGAAAAGAAAAGGCATGAATATCATGAAGCTGTCAAGAATCGCTACAAGCACCTCCCTGAAGTCAAGCGTATAGTGAGGCACAGGCACTTGCCAAAACCAATATACAAGGCAACTGGTCTCCGGCGTACTATGATCGAAGCCGAGCGAAGGAGAGAAGAGAAGAGGAAAGCTCACAGTGCCCCAGGAACCATTACATCAAAGCCATTACGGCAAACAAGAATTGTCCGTGTTGAGGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000462 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030684 GO:0031461 GO:0031974 GO:0031981 GO:0032040 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0080008 GO:0090304 GO:1901360 GO:1902494 GO:1990234 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sof1 WD40

Protein Analysis

377

Amino Acids

42.92

Weight (kDa)

9.39

Isoelectric Point (pI)

41.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	7 - 63	8.7e-08	THOC3 beta-propeller domain
WDR55	PF24796	7 - 63	3.6e-06	WDR55
Beta-prop_WDR3_1st	PF25173	7 - 79	5.3e-12	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	7 - 181	3.7e-08	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	7 - 64	2.9e-09	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	7 - 68	1.4e-06	G protein beta WD-40 repeat protein
Beta-prop_WDR5	PF25175	7 - 67	2.8e-12	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	7 - 62	3.3e-10	WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	8 - 62	1e-06	TEP-1 second beta-propeller
Beta-prop_DCAF4	PF23761	9 - 63	1.4e-06	DDB1- and CUL4-associated factor 4 beta-propeller domain
WD40_Prp19	PF24814	9 - 137	8.9e-10	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	15 - 68	1.1e-08	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	26 - 61	6.2e-09	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	92 - 181	3.9e-11	THOC3 beta-propeller domain
WD40_Prp19	PF24814	106 - 235	1.7e-08	Prp19 WD40 domain
Beta-prop_EML	PF23409	109 - 298	1.8e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_CDC20-Fz	PF24807	110 - 244	1.7e-11	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	128 - 300	2.1e-20	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	137 - 292	1e-14	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	144 - 297	3.5e-10	G protein beta WD-40 repeat protein
WDR55	PF24796	170 - 289	2.3e-08	WDR55
Beta-prop_THOC3	PF25174	170 - 293	6.2e-15	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	187 - 292	2e-10	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	190 - 292	3e-07	WDHD1 first WD40 domain
WD40	PF00400	208 - 241	2.4e-06	WD domain, G-beta repeat
WD40_Prp19	PF24814	210 - 291	1.3e-07	Prp19 WD40 domain
Sof1	PF04158	286 - 371	3.5e-30	Sof1-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0006826)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28450
fragaria_vesca	FvH4_2g10410 FvH4_2g10410
malus_domestica	MD08G1220200.v1.1 MD15G1410600.v1.1 MD15G1410700.v1.1
prunus_persica	Prupe.1G573700_v2.0.a1 Prupe.1G573700_v2.0.a1 Prupe.3G166600_v2.0.a1 Prupe.6G177000_v2.0.a1 Prupe.6G177000_v2.0.a1 Prupe.6G177000_v2.0.a1 Prupe.6G177000_v2.0.a1
pyrus_communis	pycom08g19110 pycom15g36550
rosa_chinensis	RchiOBHm_Chr6g0267881
rosa_laevigata	RLG00000013973
rosa_multiflora	Rmu_co8335391.1_g000001 Rmu_sc0000434.1_g000004 Rmu_sc0005400.1_g000008
rosa_roxburghii	Rroxscaffold_7G00200360
rosa_rugosa	Rorug06G0034600
rosa_samantha	Rh6AG156600 Rh6BG157800 Rh6DG143000
rosa_wichuraiana	Rw6G013600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	330
AciI	CCGC	2 cut(s)	242, 441
AcoI	YGGCCR	1 cut(s)	94
AcsI	RAATTY	3 cut(s)	295, 683, 717
AcuI	CTGAAG	1 cut(s)	972
AfaI	GTAC	2 cut(s)	189, 1021
AfiI	CCNNNNNNNGG	2 cut(s)	1041, 1126
AflIII	ACRYGT	2 cut(s)	139, 620
AgsI	TTSAA	2 cut(s)	33, 553
AjiI	CACGTC	1 cut(s)	140
AjnI	CCWGG	4 cut(s)	68, 91, 412, 1075
AleI	CACNNNNGTG	1 cut(s)	102
AluBI	AGCT	9 cut(s)	65, 211, 323, 499, 591, 607, 798, 923, 1064
AluI	AGCT	9 cut(s)	65, 211, 323, 499, 591, 607, 798, 923, 1064
Alw21I	GWGCWC	1 cut(s)	630
Alw26I	GTCTC	4 cut(s)	130, 154, 223, 1016
Alw44I	GTGCAC	1 cut(s)	626
AlwNI	CAGNNNCTG	1 cut(s)	180
AoxI	GGCC	2 cut(s)	94, 183
ApaLI	GTGCAC	1 cut(s)	626
ApeKI	GCWGC	3 cut(s)	453, 563, 614
ApoI	RAATTY	3 cut(s)	295, 683, 717
AspLEI	GCGC	1 cut(s)	75
AspS9I	GGNCC	1 cut(s)	183
AsuC2I	CCSGG	1 cut(s)	746
AsuHPI	GGTGA	1 cut(s)	278
BaeGI	GKGCMC	2 cut(s)	630, 1075
BalI	TGGCCA	1 cut(s)	96
Bbv12I	GWGCWC	1 cut(s)	630
BbvI	GCAGC	3 cut(s)	465, 550, 626
BccI	CCATC	4 cut(s)	32, 128, 199, 646
BceAI	ACGGC	2 cut(s)	170, 1118
BciT130I	CCWGG	4 cut(s)	70, 93, 414, 1077
BclI	TGATCA	1 cut(s)	283
BcnI	CCSGG	1 cut(s)	746
BcoDI	GTCTC	4 cut(s)	130, 154, 223, 1016
BfaI	CTAG	4 cut(s)	212, 245, 600, 807
BfmI	CTRYAG	2 cut(s)	129, 586
BglI	GCCNNNNNGGC	1 cut(s)	1102
BisI	GCNGC	4 cut(s)	242, 454, 564, 615
BlsI	GCNGC	4 cut(s)	243, 455, 565, 616
Bme1390I	CCNGG	5 cut(s)	70, 93, 414, 746, 1077
BmgBI	CACGTC	1 cut(s)	140
BmgT120I	GGNCC	1 cut(s)	183
BmiI	GGNNCC	4 cut(s)	341, 536, 546, 1081
BmrFI	CCNGG	5 cut(s)	70, 93, 414, 746, 1077
BmrI	ACTGGG	1 cut(s)	702
BmsI	GCATC	2 cut(s)	793, 872
BmuI	ACTGGG	1 cut(s)	702
BplI	GAGNNNNNCTC	2 cut(s)	1048, 1080
BpuMI	CCSGG	1 cut(s)	746
BsaI	GGTCTC	1 cut(s)	1016
BsaJI	CCNNGG	3 cut(s)	100, 890, 1075
BsaWI	WCCGGW	3 cut(s)	26, 358, 402
BsaXI	ACNNNNNCTCC	2 cut(s)	312, 342
Bsc4I	CCNNNNNNNGG	2 cut(s)	1041, 1126
Bse1I	ACTGG	4 cut(s)	679, 697, 724, 1012
BseBI	CCWGG	4 cut(s)	70, 93, 414, 1077
BseDI	CCNNGG	3 cut(s)	100, 890, 1075
BseGI	GGATG	2 cut(s)	43, 437
BseLI	CCNNNNNNNGG	2 cut(s)	1041, 1126
BseNI	ACTGG	4 cut(s)	679, 697, 724, 1012
BseSI	GKGCMC	2 cut(s)	630, 1075
BseXI	GCAGC	3 cut(s)	465, 550, 626
BsgI	GTGCAG	1 cut(s)	339
BshFI	GGCC	2 cut(s)	96, 185
BsiHKAI	GWGCWC	1 cut(s)	630
BsiSI	CCGG	5 cut(s)	27, 359, 403, 745, 1015
BslI	CCNNNNNNNGG	2 cut(s)	1041, 1126
BsmAI	GTCTC	4 cut(s)	130, 154, 223, 1016
BsmI	GAATGC	2 cut(s)	271, 774
BsnI	GGCC	2 cut(s)	96, 185
Bso31I	GGTCTC	1 cut(s)	1016
Bsp1286I	GDGCHC	2 cut(s)	630, 1075
Bsp143I	GATC	6 cut(s)	283, 301, 424, 448, 634, 1026
BspACI	CCGC	2 cut(s)	242, 441
BspANI	GGCC	2 cut(s)	96, 185
BspHI	TCATGA	1 cut(s)	916
BspLI	GGNNCC	4 cut(s)	341, 536, 546, 1081
BspTNI	GGTCTC	1 cut(s)	1016
BsrI	ACTGG	4 cut(s)	679, 697, 724, 1012
BssECI	CCNNGG	3 cut(s)	100, 890, 1075
BssMI	GATC	6 cut(s)	283, 301, 424, 448, 634, 1026
BssT1I	CCWWGG	2 cut(s)	100, 890
Bst2UI	CCWGG	4 cut(s)	70, 93, 414, 1077
Bst4CI	ACNGT	5 cut(s)	79, 391, 712, 873, 1070
Bst6I	CTCTTC	2 cut(s)	1044, 1049
BstDEI	CTNAG	1 cut(s)	842
BstF5I	GGATG	2 cut(s)	43, 437
BstHHI	GCGC	1 cut(s)	75
BstKTI	GATC	6 cut(s)	286, 304, 427, 451, 637, 1029
BstMAI	GTCTC	4 cut(s)	130, 154, 223, 1016
BstMBI	GATC	6 cut(s)	283, 301, 424, 448, 634, 1026
BstMWI	GCNNNNNNNGC	3 cut(s)	440, 1070, 1102
BstNI	CCWGG	4 cut(s)	70, 93, 414, 1077
BstSCI	CCNGG	5 cut(s)	68, 91, 412, 744, 1075
BstSFI	CTRYAG	2 cut(s)	129, 586
BstSLI	GKGCMC	2 cut(s)	630, 1075
BstV1I	GCAGC	3 cut(s)	465, 550, 626
BstXI	CCANNNNNNTGG	1 cut(s)	731
BsuRI	GGCC	2 cut(s)	96, 185
BtgZI	GCGATG	1 cut(s)	218
BtrI	CACGTC	1 cut(s)	140
BtsCI	GGATG	2 cut(s)	43, 437
BtsI	GCAGTG	2 cut(s)	175, 558
BtsIMutI	CAGTG	4 cut(s)	175, 558, 717, 1075
CaiI	CAGNNNCTG	1 cut(s)	180
CciI	TCATGA	1 cut(s)	916
CfoI	GCGC	1 cut(s)	75
Cfr13I	GGNCC	1 cut(s)	183
CseI	GACGC	1 cut(s)	775
Csp6I	GTAC	2 cut(s)	188, 1020
CviAII	CATG	5 cut(s)	478, 505, 638, 908, 917
CviQI	GTAC	2 cut(s)	188, 1020
DdeI	CTNAG	1 cut(s)	842
DpnI	GATC	6 cut(s)	285, 303, 426, 450, 636, 1028
DpnII	GATC	6 cut(s)	283, 301, 424, 448, 634, 1026
EaeI	YGGCCR	1 cut(s)	94
Eam1104I	CTCTTC	2 cut(s)	1044, 1049
EarI	CTCTTC	2 cut(s)	1044, 1049
Eco130I	CCWWGG	2 cut(s)	100, 890
Eco31I	GGTCTC	1 cut(s)	1016
Eco57I	CTGAAG	1 cut(s)	972
EcoRII	CCWGG	4 cut(s)	68, 91, 412, 1075
EcoT14I	CCWWGG	2 cut(s)	100, 890
EcoT22I	ATGCAT	1 cut(s)	271
ErhI	CCWWGG	2 cut(s)	100, 890
FaeI	CATG	5 cut(s)	481, 508, 641, 911, 920
FatI	CATG	5 cut(s)	477, 504, 637, 907, 916
FauNDI	CATATG	1 cut(s)	335
FbaI	TGATCA	1 cut(s)	283
FblI	GTMKAC	1 cut(s)	330
Fnu4HI	GCNGC	4 cut(s)	242, 454, 564, 615
FokI	GGATG	2 cut(s)	50, 424
Fsp4HI	GCNGC	4 cut(s)	242, 454, 564, 615
FspBI	CTAG	4 cut(s)	212, 245, 600, 807
GlaI	GCGC	1 cut(s)	74
GluI	GCNGC	4 cut(s)	242, 454, 564, 615
HaeIII	GGCC	2 cut(s)	96, 185
HapII	CCGG	5 cut(s)	27, 359, 403, 745, 1015
HgaI	GACGC	1 cut(s)	775
HhaI	GCGC	1 cut(s)	75
Hin1II	CATG	5 cut(s)	481, 508, 641, 911, 920
Hin6I	GCGC	1 cut(s)	73
HinP1I	GCGC	1 cut(s)	73
HindIII	AAGCTT	2 cut(s)	497, 605
HinfI	GANTC	3 cut(s)	596, 838, 931
HpaII	CCGG	5 cut(s)	27, 359, 403, 745, 1015
HphI	GGTGA	1 cut(s)	278
Hpy166II	GTNNAC	2 cut(s)	331, 628
Hpy188I	TCNGA	2 cut(s)	226, 868
Hpy188III	TCNNGA	3 cut(s)	600, 917, 928
Hpy8I	GTNNAC	2 cut(s)	331, 628
HpyAV	CCTTC	2 cut(s)	847, 1035
HpyCH4III	ACNGT	5 cut(s)	79, 391, 712, 873, 1070
HpyCH4IV	ACGT	2 cut(s)	139, 620
HpyCH4V	TGCA	8 cut(s)	168, 269, 356, 566, 579, 614, 628, 772
HpyF10VI	GCNNNNNNNGC	3 cut(s)	440, 1070, 1102
HpyF3I	CTNAG	1 cut(s)	842
HpySE526I	ACGT	2 cut(s)	139, 620
Hsp92II	CATG	5 cut(s)	481, 508, 641, 911, 920
HspAI	GCGC	1 cut(s)	73
Ksp22I	TGATCA	1 cut(s)	283
Kzo9I	GATC	6 cut(s)	283, 301, 424, 448, 634, 1026
LmnI	GCTCC	3 cut(s)	208, 320, 544
Lsp1109I	GCAGC	3 cut(s)	465, 550, 626
LweI	GCATC	2 cut(s)	793, 872
MaeI	CTAG	4 cut(s)	212, 245, 600, 807
MaeII	ACGT	2 cut(s)	139, 620
MaeIII	GTNAC	2 cut(s)	688, 737
MalI	GATC	6 cut(s)	285, 303, 426, 450, 636, 1028
MboI	GATC	6 cut(s)	283, 301, 424, 448, 634, 1026
MboII	GAAGA	5 cut(s)	274, 875, 878, 1061, 1066
MfeI	CAATTG	1 cut(s)	580
MhlI	GDGCHC	2 cut(s)	630, 1075
MlsI	TGGCCA	1 cut(s)	96
MluCI	AATT	6 cut(s)	295, 520, 580, 683, 717, 1113
MluNI	TGGCCA	1 cut(s)	96
MmeI	TCCRAC	2 cut(s)	512, 695
MnlI	CCTC	6 cut(s)	107, 565, 956, 963, 1050, 1120
Mox20I	TGGCCA	1 cut(s)	96
Mph1103I	ATGCAT	1 cut(s)	271
MscI	TGGCCA	1 cut(s)	96
MseI	TTAA	2 cut(s)	408, 609
MslI	CAYNNNNRTG	1 cut(s)	102
Msp20I	TGGCCA	1 cut(s)	96
MspA1I	CMGCKG	1 cut(s)	798
MspI	CCGG	5 cut(s)	27, 359, 403, 745, 1015
MspR9I	CCNGG	5 cut(s)	70, 93, 414, 746, 1077
MunI	CAATTG	1 cut(s)	580
Mva1269I	GAATGC	2 cut(s)	271, 774
MvaI	CCWGG	4 cut(s)	70, 93, 414, 1077
MwoI	GCNNNNNNNGC	3 cut(s)	440, 1070, 1102
NciI	CCSGG	1 cut(s)	746
NdeI	CATATG	1 cut(s)	335
NdeII	GATC	6 cut(s)	283, 301, 424, 448, 634, 1026
NlaIII	CATG	5 cut(s)	481, 508, 641, 911, 920
NlaIV	GGNNCC	4 cut(s)	341, 536, 546, 1081
NmeAIII	GCCGAG	1 cut(s)	1060
NmuCI	GTSAC	1 cut(s)	737
NsiI	ATGCAT	1 cut(s)	271
OliI	CACNNNNGTG	1 cut(s)	102
PagI	TCATGA	1 cut(s)	916
PctI	GAATGC	2 cut(s)	271, 774
PfeI	GAWTC	3 cut(s)	596, 838, 931
PfoI	TCCNGGA	1 cut(s)	412
PkrI	GCNGC	4 cut(s)	243, 455, 565, 616
Psp6I	CCWGG	4 cut(s)	68, 91, 412, 1075
PspGI	CCWGG	4 cut(s)	68, 91, 412, 1075
PspN4I	GGNNCC	4 cut(s)	341, 536, 546, 1081
PspPI	GGNCC	1 cut(s)	183
PstNI	CAGNNNCTG	1 cut(s)	180
PvuII	CAGCTG	1 cut(s)	798
RsaI	GTAC	2 cut(s)	189, 1021
RsaNI	GTAC	2 cut(s)	188, 1020
RseI	CAYNNNNRTG	1 cut(s)	102
SaqAI	TTAA	2 cut(s)	408, 609
SatI	GCNGC	4 cut(s)	242, 454, 564, 615
Sau3AI	GATC	6 cut(s)	283, 301, 424, 448, 634, 1026
Sau96I	GGNCC	1 cut(s)	183
ScrFI	CCNGG	5 cut(s)	70, 93, 414, 746, 1077
SduI	GDGCHC	2 cut(s)	630, 1075
SfaNI	GCATC	2 cut(s)	793, 872
SfcI	CTRYAG	2 cut(s)	129, 586
SmiMI	CAYNNNNRTG	1 cut(s)	102
Sse9I	AATT	6 cut(s)	295, 520, 580, 683, 717, 1113
SsiI	CCGC	2 cut(s)	242, 441
SspI	AATATT	1 cut(s)	21
SspMI	CTAG	4 cut(s)	212, 245, 600, 807
StyD4I	CCNGG	5 cut(s)	68, 91, 412, 744, 1075
StyI	CCWWGG	2 cut(s)	100, 890
TaaI	ACNGT	5 cut(s)	79, 391, 712, 873, 1070
TaiI	ACGT	2 cut(s)	142, 623
TaqI	TCGA	1 cut(s)	1029
TaqII	GACCGA	1 cut(s)	264
TasI	AATT	6 cut(s)	295, 520, 580, 683, 717, 1113
TauI	GCSGC	1 cut(s)	244
TfiI	GAWTC	3 cut(s)	596, 838, 931
Tru1I	TTAA	2 cut(s)	408, 609
Tru9I	TTAA	2 cut(s)	408, 609
TscAI	CASTG	4 cut(s)	175, 565, 717, 1075
TseFI	GTSAC	1 cut(s)	737
TseI	GCWGC	3 cut(s)	453, 563, 614
Tsp45I	GTSAC	1 cut(s)	737
TspDTI	ATGAA	4 cut(s)	17, 71, 924, 933
TspGWI	ACGGA	1 cut(s)	1109
TspRI	CASTG	4 cut(s)	175, 565, 717, 1075
VneI	GTGCAC	1 cut(s)	626
XapI	RAATTY	3 cut(s)	295, 683, 717
XbaI	TCTAGA	1 cut(s)	599
XcmI	CCANNNNNNNNNTGG	1 cut(s)	731
XmiI	GTMKAC	1 cut(s)	330
XspI	CTAG	4 cut(s)	212, 245, 600, 807
Zsp2I	ATGCAT	1 cut(s)	271

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.