Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002023

Physical Location & Seq

Genomic Coordinates Reverse (-)

84063 .. 91882

Gene Structure 7820 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1000400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2499 bp

ATGGTCCCCCTTCAGCATCATCAGTTATATTACAATAATAATATTAATATTAATATTAATAACACTGTGACTACCTGTACCTGGGGAGGAGGAAGAGGAGGAGGATTTGGATTAGGTTATAGTAATCTTCATAATGTTATCCTTTATCCGGGTCAGGGTTTTGCATGTCATCAGATTCATCATCACACTCCTCCGTTAGCCAAACATTCCGTTTCCGCTCTCCACGAAAGAAGAAAGTCAATTCCAGATTCCAATTCCAATTCGTTGTGTCGCCTTCTTATACCAAGAGGAAGAACAAGGAGGAGCTGTTTGTGGGCGTCGCCCAAATTTAAATCTTGCGGTTGGGACATGGAATCATCATCGCCTGATCATGCTGCTAATAACAACAAGAAGCCTCGTAATAATCCAAATTTGATTACAACAAGCGATCACCTGCGGCATGTGGAGTCGATGGCCACAATGCCTTCTGGCGCCGGAAACATTTCCCACCTGAACGCCATCATTCTCGGAGAATCACTCGCCTCTGAAGAGAACGATCTCGTCTTTCCCAGCGACCACTTCTCCCGTCAGGCTCACGTCTCCTCTCCCCAACAGTATCTGGAGATGTATAAACGGTCCATCGAGGACCCCGCCGGATTTTGGTCCGACATCGCTTCCACCTTCTTTTGGAAACAGAAATGGGGCAAACCAGTCTACTCGGAGAATCTCGATATCAGGAAAGGCGATATCCGGATTGAGTGGTTCAAGGGCGGTCTCACCAATATCTGCTACAACTGCCTTGACACAAATGTTGAAGCTGGACTTGGCGACAAAATTGCCTTCTACTGGGAAGGCAATGATCTTGGTGTTGATGCCACTTTAACTTACACCCAGCTTCTCCACAACGTTTGCCAGCTTGCAAACTATTTGAAAGATGTTGGAGTTAAAAAGGGTGATGCTGTTGTGGTTTACCTGCCCATGTTAATGGAACTTCCAATCACAATGCTTGCCTGTGCTCGAATTGGTGCCGTTCACTCGGTTGTCTTTGCCGGATTTTCTGCAGAATCTCTTGCTCAAAGGATTGTAGATTGCAAACCGAAAGTCGTAATCACTTGCAATGCTGTTAAAAGAGGTTCTAAGGTTATCCACCTCAAAGATATAGTTGATGCTGCCCTTATAAAATCATCCGAAAGCGGGGTTTCTGTAGATATATGCTTAACTTACGAAAATCAATCGGCTATGAAGAGGGAAAGTACTAAATGGCTGGAAGGAAGGGATGTATGCTGGCAGGATGTCATACCTAAATATCCAACTACTTGTGCAGTGGAGTGGGTTGATGCGGAAGATCCACTTTTTCTGCTCTATACCAGTGGGAGCACTGGTAAACCGAAGGGAGTTCTCCACACAACTGGAGGATATATGGTGTACGCTGCAACAACTTTTAAATATGCATTTGATTACAAGTCATCTGACATCTACTGGTGTACAGCTGACTGTGGTTGGATCACTGGGCACAGCTATGTCACTTATGGACCCCTGCTAAATGGAGCGACTGCTGTTTTATATGAAGGGGCTCCAAATTATCCTGACCCTGGACGCTGTTGGGATGTTGTTGATAAATACAAAGTGAGCATATTTTATACTGCCCCTACATTGGTGCGGTCCCTCATGCGTGATAGTGATGAGTATGTTACACGATACTCGCGAAAATCCTTACGGGTCCTTGGAAGTGTAGGCGAGCCTATTAATCCAAGTGCATGGAGGTGGTTTTTCAATGTGGTTGGAGATTCAAAATGTCCTATTTCTGACACTTGGTGGCAAACTGAAACTGGTGGCTTCATGATAACTCCATTACCGGGTGCCTGGCCACAGAAGCCTGGTTCTGCTACTTTCCCTTTCTTTGGGGTTCAGGCTGTCATAGTTGATGAAAAGGGTGTTGAGATTGAAGGGGAATGCAGTGGTTATCTGTGTGTAAAAAGCTCATGGCCTGGAGCCTTCCGAACTCTGTACGGTGATCATGAAAGATACGAAACCACTTACTTTAAACCTTTCCCTGGGTATTATTTTAGTGGTGATGGATGCAGCAGGGACAAGGATGGATATCACTGGCTTACAGGAAGGGTTGATGATGTTATCAACGTTAGTGGACATCGTATTGGTACAGCAGAAGTGGAATCTGCTCTGGTCTCACATCCCCAGTGTGCTGAAGCTGCTGTGGTTGGCGTTGAGCATGAGGTTAAAGGACAAGGTATATATGCCTTTGTTACTCTTGTGGAAGGTGTACCTTACAGTGAGGAACTCCGGAAAAGCCTTATACTGGCTGTGAGAAAGCAGATAGGACCATTTGCAGCACCGGACAAAATCCACTGGGCCCCTGGCCTTCCAAAAACAAGGAGTGGGAAGATCATGAGGAGGATTCTGAGGAAGATTGCTTCTAGACAGTTAGATGAGCTTGGAGACACCAGCACCCTTGCGGAACCAAATGTGGTCGATCAACTAATTGCTCTCGCAGATGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003987 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006082 GO:0006083 GO:0008150 GO:0008152 GO:0009507 GO:0009514 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017144 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00010 ko00620 ko00640 ko00680 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00620 map00640 map00680 map00720 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

ACAS_N AMP-binding AMP-binding_C

Protein Analysis

833

Amino Acids

92.36

Weight (kDa)

6.81

Isoelectric Point (pI)

37.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACAS_N	PF16177	199 - 259	7.4e-18	Acetyl-coenzyme A synthetase N-terminus
AMP-binding	PF00501	268 - 654	3.6e-90	AMP-binding enzyme
AMP-binding_C	PF13193	716 - 794	7.1e-25	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G36880 AT5G36880 AT5G36880 AT5G36880 AT5G36880 AT5G36880
fragaria_vesca	FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480
malus_domestica	MD09G1000400.v1.1 MD17G1003300.v1.1
prunus_persica	Prupe.3G315800_v2.0.a1
pyrus_communis	pycom111g00030 pycom17g00010
rosa_chinensis	RchiOBHm_Chr2g0176771
rosa_multiflora	Rmu_sc0020992.1_g000001
rosa_roxburghii	Rroxscaffold_2G00076170
rosa_rugosa	Rorug02G0598400 Rorug02G0598500 Rorug02G0598600 Rorug02G0598700 Rorug02G0598800 Rorug02G0598900 Rorug02G0599000 Rorug02G0599100 Rorug02G0599200
rosa_samantha	Rh2AG676000 Rh2BG687100 Rh2CG650500
rosa_wichuraiana	Rw2G055470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1157
AarI	CACCTGC	1 cut(s)	441
Acc36I	ACCTGC	2 cut(s)	441, 960
AccB1I	GGYRCC	3 cut(s)	470, 1004, 1838
AccBSI	CCGCTC	1 cut(s)	218
AccI	GTMKAC	1 cut(s)	693
AccII	CGCG	1 cut(s)	1684
AccIII	TCCGGA	2 cut(s)	729, 2280
AciI	CCGC	9 cut(s)	216, 339, 436, 630, 750, 1173, 1319, 1639, 2453
AclI	AACGTT	2 cut(s)	885, 2118
AclWI	GGATC	2 cut(s)	1319, 1490
AcoI	YGGCCR	2 cut(s)	453, 1844
AcsI	RAATTY	2 cut(s)	326, 409
AcuI	CTGAAG	2 cut(s)	546, 2205
AcyI	GRCGYC	2 cut(s)	317, 471
AdeI	CACNNNGTG	1 cut(s)	1794
AfaI	GTAC	7 cut(s)	79, 1234, 1406, 1465, 1988, 2140, 2262
AgsI	TTSAA	6 cut(s)	745, 794, 910, 1753, 1770, 1925
AjiI	CACGTC	1 cut(s)	577
AjnI	CCWGG	7 cut(s)	80, 1570, 1841, 1855, 1966, 2032, 2353
AjuI	GAANNNNNNNTTGG	2 cut(s)	786, 818
AluBI	AGCT	9 cut(s)	306, 797, 874, 895, 1469, 1497, 1959, 2189, 2431
AluI	AGCT	9 cut(s)	306, 797, 874, 895, 1469, 1497, 1959, 2189, 2431
Alw21I	GWGCWC	2 cut(s)	997, 1358
Alw26I	GTCTC	4 cut(s)	583, 758, 2170, 2430
AlwI	GGATC	2 cut(s)	1319, 1490
AlwNI	CAGNNNCTG	1 cut(s)	598
Aor13HI	TCCGGA	2 cut(s)	729, 2280
AoxI	GGCC	5 cut(s)	453, 1844, 1964, 2349, 2356
ApaI	GGGCCC	1 cut(s)	2353
ApeKI	GCWGC	6 cut(s)	374, 1148, 1409, 2061, 2189, 2327
ApoI	RAATTY	2 cut(s)	326, 409
ArsI	GACNNNNNNTTYG	2 cut(s)	1065, 1097
AseI	ATTAAT	4 cut(s)	45, 51, 57, 1725
Asp700I	GAANNNNTTC	1 cut(s)	481
AspLEI	GCGC	1 cut(s)	473
AsuC2I	CCSGG	2 cut(s)	150, 1836
AsuHPI	GGTGA	5 cut(s)	422, 748, 944, 2003, 2063
AvaII	GGWCC	8 cut(s)	4, 615, 625, 642, 1511, 1641, 1699, 2318
BaeGI	GKGCMC	2 cut(s)	1494, 2353
BalI	TGGCCA	2 cut(s)	455, 1846
BanI	GGYRCC	3 cut(s)	470, 1004, 1838
BanII	GRGCYC	2 cut(s)	1555, 2353
Bbv12I	GWGCWC	2 cut(s)	997, 1358
BbvI	GCAGC	6 cut(s)	361, 1135, 1396, 2073, 2176, 2339
BccI	CCATC	5 cut(s)	445, 506, 626, 2048, 2069
BceAI	ACGGC	1 cut(s)	992
BcgI	CGANNNNNNTGC	2 cut(s)	797, 831
BciT130I	CCWGG	7 cut(s)	82, 1572, 1843, 1857, 1968, 2034, 2355
BclI	TGATCA	2 cut(s)	367, 1993
BcnI	CCSGG	2 cut(s)	150, 1836
BcoDI	GTCTC	4 cut(s)	583, 758, 2170, 2430
BfaI	CTAG	2 cut(s)	2415, 2497
BfmI	CTRYAG	2 cut(s)	1038, 1182
BfoI	RGCGCY	1 cut(s)	474
BfuAI	ACCTGC	2 cut(s)	441, 960
BisI	GCNGC	7 cut(s)	375, 437, 1149, 1410, 2062, 2190, 2328
BlsI	GCNGC	7 cut(s)	376, 438, 1150, 1411, 2063, 2191, 2329
BmcAI	AGTACT	1 cut(s)	1234
Bme1390I	CCNGG	9 cut(s)	82, 150, 1572, 1836, 1843, 1857, 1968, 2034, 2355
Bme18I	GGWCC	8 cut(s)	4, 615, 625, 642, 1511, 1641, 1699, 2318
BmgBI	CACGTC	1 cut(s)	577
BmrFI	CCNGG	9 cut(s)	82, 150, 1572, 1836, 1843, 1857, 1968, 2034, 2355
BmrI	ACTGGG	4 cut(s)	835, 1497, 2170, 2356
BmsI	GCATC	6 cut(s)	25, 841, 925, 1135, 1306, 2048
BmuI	ACTGGG	4 cut(s)	835, 1497, 2170, 2356
BplI	GAGNNNNNCTC	2 cut(s)	501, 533
BpmI	CTGGAG	3 cut(s)	620, 1410, 1989
BpuMI	CCSGG	2 cut(s)	150, 1836
BsaBI	GATNNNNATC	1 cut(s)	2079
BsaHI	GRCGYC	2 cut(s)	317, 471
BsaI	GGTCTC	2 cut(s)	758, 2170
BsaJI	CCNNGG	6 cut(s)	81, 1570, 1702, 2032, 2033, 2353
BsaWI	WCCGGW	3 cut(s)	729, 2280, 2332
BsaXI	ACNNNNNCTCC	6 cut(s)	292, 322, 545, 575, 1345, 1375
Bse3DI	GCAATG	2 cut(s)	841, 1102
Bse8I	GATNNNNATC	1 cut(s)	2079
BseAI	TCCGGA	2 cut(s)	729, 2280
BseBI	CCWGG	7 cut(s)	82, 1572, 1843, 1857, 1968, 2034, 2355
BseDI	CCNNGG	6 cut(s)	81, 1570, 1702, 2032, 2033, 2353
BseGI	GGATG	7 cut(s)	1163, 1261, 1276, 1591, 2063, 2080, 2170
BseJI	GATNNNNATC	1 cut(s)	2079
BseMI	GCAATG	2 cut(s)	841, 1102
BseMII	CTCAG	1 cut(s)	2390
BseRI	GAGGAG	7 cut(s)	102, 111, 114, 180, 316, 571, 2404
BseSI	GKGCMC	2 cut(s)	1494, 2353
BseXI	GCAGC	6 cut(s)	361, 1135, 1396, 2073, 2176, 2339
BseYI	CCCAGC	2 cut(s)	548, 870
BsgI	GTGCAG	1 cut(s)	1320
Bsh1236I	CGCG	1 cut(s)	1684
BshFI	GGCC	5 cut(s)	455, 1846, 1966, 2351, 2358
BshNI	GGYRCC	3 cut(s)	470, 1004, 1838
BsiHKAI	GWGCWC	2 cut(s)	997, 1358
BsiSI	CCGG	8 cut(s)	149, 474, 633, 730, 1029, 1835, 2281, 2333
BslFI	GGGAC	3 cut(s)	359, 1627, 2081
BsmAI	GTCTC	4 cut(s)	583, 758, 2170, 2430
BsmBI	CGTCTC	1 cut(s)	583
BsmFI	GGGAC	3 cut(s)	359, 1627, 2081
BsmI	GAATGC	1 cut(s)	1937
BsnI	GGCC	5 cut(s)	455, 1846, 1966, 2351, 2358
Bso31I	GGTCTC	2 cut(s)	758, 2170
Bsp120I	GGGCCC	1 cut(s)	2349
Bsp1286I	GDGCHC	5 cut(s)	997, 1358, 1494, 1555, 2353
Bsp13I	TCCGGA	2 cut(s)	729, 2280
Bsp1407I	TGTACA	1 cut(s)	1463
Bsp143I	GATC	9 cut(s)	367, 427, 535, 838, 1324, 1482, 1993, 2382, 2470
Bsp68I	TCGCGA	1 cut(s)	1684
BspACI	CCGC	9 cut(s)	216, 339, 436, 630, 750, 1173, 1319, 1639, 2453
BspANI	GGCC	5 cut(s)	455, 1846, 1966, 2351, 2358
BspCNI	CTCAG	1 cut(s)	2391
BspEI	TCCGGA	2 cut(s)	729, 2280
BspFNI	CGCG	1 cut(s)	1684
BspHI	TCATGA	3 cut(s)	1818, 1996, 2385
BspMAI	CTGCAG	1 cut(s)	1042
BspMI	ACCTGC	2 cut(s)	441, 960
BspPI	GGATC	2 cut(s)	1319, 1490
BspT107I	GGYRCC	3 cut(s)	470, 1004, 1838
BspTNI	GGTCTC	2 cut(s)	758, 2170
BsrBI	CCGCTC	1 cut(s)	218
BsrDI	GCAATG	2 cut(s)	841, 1102
BsrGI	TGTACA	1 cut(s)	1463
BssECI	CCNNGG	6 cut(s)	81, 1570, 1702, 2032, 2033, 2353
BssMI	GATC	9 cut(s)	367, 427, 535, 838, 1324, 1482, 1993, 2382, 2470
BssNI	GRCGYC	2 cut(s)	317, 471
BssT1I	CCWWGG	1 cut(s)	1702
Bst2UI	CCWGG	7 cut(s)	82, 1572, 1843, 1857, 1968, 2034, 2355
Bst4CI	ACNGT	7 cut(s)	67, 594, 615, 1475, 1991, 2270, 2421
Bst6I	CTCTTC	3 cut(s)	88, 522, 1217
BstACI	GRCGYC	2 cut(s)	317, 471
BstAUI	TGTACA	1 cut(s)	1463
BstC8I	GCNNGC	5 cut(s)	893, 897, 987, 1265, 1718
BstDEI	CTNAG	2 cut(s)	1116, 2399
BstF5I	GGATG	7 cut(s)	1163, 1261, 1276, 1591, 2063, 2080, 2170
BstFNI	CGCG	1 cut(s)	1684
BstH2I	RGCGCY	1 cut(s)	474
BstHHI	GCGC	1 cut(s)	473
BstKTI	GATC	9 cut(s)	370, 430, 538, 841, 1327, 1485, 1996, 2385, 2473
BstMAI	GTCTC	4 cut(s)	583, 758, 2170, 2430
BstMBI	GATC	9 cut(s)	367, 427, 535, 838, 1324, 1482, 1993, 2382, 2470
BstMWI	GCNNNNNNNGC	3 cut(s)	774, 1852, 2189
BstNI	CCWGG	7 cut(s)	82, 1572, 1843, 1857, 1968, 2034, 2355
BstNSI	RCATGY	2 cut(s)	168, 443
BstSCI	CCNGG	9 cut(s)	80, 148, 1570, 1834, 1841, 1855, 1966, 2032, 2353
BstSFI	CTRYAG	2 cut(s)	1038, 1182
BstSLI	GKGCMC	2 cut(s)	1494, 2353
BstUI	CGCG	1 cut(s)	1684
BstV1I	GCAGC	6 cut(s)	361, 1135, 1396, 2073, 2176, 2339
BstX2I	RGATCY	1 cut(s)	1324
BstXI	CCANNNNNNTGG	3 cut(s)	964, 1388, 1737
BstYI	RGATCY	1 cut(s)	1324
BsuRI	GGCC	5 cut(s)	455, 1846, 1966, 2351, 2358
BtgZI	GCGATG	2 cut(s)	345, 634
BtrI	CACGTC	1 cut(s)	577
BtsCI	GGATG	7 cut(s)	1163, 1261, 1276, 1591, 2063, 2080, 2170
BtsI	GCAGTG	2 cut(s)	1308, 1942
BtuMI	TCGCGA	1 cut(s)	1684
BveI	ACCTGC	2 cut(s)	441, 960
Cac8I	GCNNGC	5 cut(s)	893, 897, 987, 1265, 1718
CaiI	CAGNNNCTG	1 cut(s)	598
CciI	TCATGA	3 cut(s)	1818, 1996, 2385
CfoI	GCGC	1 cut(s)	473
CseI	GACGC	2 cut(s)	306, 1584
Csp6I	GTAC	7 cut(s)	78, 1233, 1405, 1464, 1987, 2139, 2261
CspCI	CAANNNNNGTGG	4 cut(s)	869, 904, 2104, 2139
CviQI	GTAC	7 cut(s)	78, 1233, 1405, 1464, 1987, 2139, 2261
DdeI	CTNAG	2 cut(s)	1116, 2399
DinI	GGCGCC	1 cut(s)	472
DpnI	GATC	9 cut(s)	369, 429, 537, 840, 1326, 1484, 1995, 2384, 2472
DpnII	GATC	9 cut(s)	367, 427, 535, 838, 1324, 1482, 1993, 2382, 2470
DraI	TTTAAA	3 cut(s)	331, 1423, 2023
DraIII	CACNNNGTG	1 cut(s)	1794
EaeI	YGGCCR	2 cut(s)	453, 1844
Eam1104I	CTCTTC	3 cut(s)	88, 522, 1217
EarI	CTCTTC	3 cut(s)	88, 522, 1217
Eco130I	CCWWGG	1 cut(s)	1702
Eco24I	GRGCYC	2 cut(s)	1555, 2353
Eco31I	GGTCTC	2 cut(s)	758, 2170
Eco32I	GATATC	3 cut(s)	712, 727, 2081
Eco47I	GGWCC	8 cut(s)	4, 615, 625, 642, 1511, 1641, 1699, 2318
Eco57I	CTGAAG	2 cut(s)	546, 2205
EcoO109I	RGGNCCY	3 cut(s)	625, 1699, 2350
EcoRII	CCWGG	7 cut(s)	80, 1570, 1841, 1855, 1966, 2032, 2353
EcoRV	GATATC	3 cut(s)	712, 727, 2081
EcoT14I	CCWWGG	1 cut(s)	1702
EcoT22I	ATGCAT	1 cut(s)	1432
EcoT38I	GRGCYC	2 cut(s)	1555, 2353
EgeI	GGCGCC	1 cut(s)	472
EheI	GGCGCC	1 cut(s)	472
ErhI	CCWWGG	1 cut(s)	1702
Esp3I	CGTCTC	1 cut(s)	583
FalI	AAGNNNNNCTT	4 cut(s)	786, 818, 1314, 1346
FaqI	GGGAC	3 cut(s)	359, 1627, 2081
FauI	CCCGC	2 cut(s)	637, 1166
FbaI	TGATCA	2 cut(s)	367, 1993
FblI	GTMKAC	1 cut(s)	693
Fnu4HI	GCNGC	7 cut(s)	375, 437, 1149, 1410, 2062, 2190, 2328
FokI	GGATG	7 cut(s)	1150, 1268, 1283, 1598, 2070, 2087, 2157
FriOI	GRGCYC	2 cut(s)	1555, 2353
Fsp4HI	GCNGC	7 cut(s)	375, 437, 1149, 1410, 2062, 2190, 2328
FspBI	CTAG	2 cut(s)	2415, 2497
GlaI	GCGC	1 cut(s)	472
GluI	GCNGC	7 cut(s)	375, 437, 1149, 1410, 2062, 2190, 2328
GsaI	CCCAGC	2 cut(s)	552, 874
GsuI	CTGGAG	3 cut(s)	620, 1410, 1989
HaeII	RGCGCY	1 cut(s)	474
HaeIII	GGCC	5 cut(s)	455, 1846, 1966, 2351, 2358
HapII	CCGG	8 cut(s)	149, 474, 633, 730, 1029, 1835, 2281, 2333
HgaI	GACGC	2 cut(s)	306, 1584
HhaI	GCGC	1 cut(s)	473
Hin1I	GRCGYC	2 cut(s)	317, 471
Hin6I	GCGC	1 cut(s)	471
HinP1I	GCGC	1 cut(s)	471
HpaII	CCGG	8 cut(s)	149, 474, 633, 730, 1029, 1835, 2281, 2333
HphI	GGTGA	5 cut(s)	422, 748, 944, 2003, 2063
Hpy166II	GTNNAC	8 cut(s)	694, 949, 1012, 1364, 1405, 1464, 2126, 2261
Hpy8I	GTNNAC	8 cut(s)	694, 949, 1012, 1364, 1405, 1464, 2126, 2261
Hpy99I	CGWCG	1 cut(s)	322
HpyCH4III	ACNGT	7 cut(s)	67, 594, 615, 1475, 1991, 2270, 2421
HpyCH4IV	ACGT	3 cut(s)	576, 885, 2118
HpyF10VI	GCNNNNNNNGC	3 cut(s)	774, 1852, 2189
HpyF3I	CTNAG	2 cut(s)	1116, 2399
HpySE526I	ACGT	3 cut(s)	576, 885, 2118
Hsp92I	GRCGYC	2 cut(s)	317, 471
HspAI	GCGC	1 cut(s)	471
KasI	GGCGCC	1 cut(s)	470
Kpn2I	TCCGGA	2 cut(s)	729, 2280
Ksp22I	TGATCA	2 cut(s)	367, 1993
Kzo9I	GATC	9 cut(s)	367, 427, 535, 838, 1324, 1482, 1993, 2382, 2470
LmnI	GCTCC	5 cut(s)	303, 1353, 1526, 1558, 1970
Lsp1109I	GCAGC	6 cut(s)	361, 1135, 1396, 2073, 2176, 2339
LweI	GCATC	6 cut(s)	25, 841, 925, 1135, 1306, 2048
MaeI	CTAG	2 cut(s)	2415, 2497
MaeII	ACGT	3 cut(s)	576, 885, 2118
MaeIII	GTNAC	4 cut(s)	67, 1501, 1669, 2242
MalI	GATC	9 cut(s)	369, 429, 537, 840, 1326, 1484, 1995, 2384, 2472
MbiI	CCGCTC	1 cut(s)	218
MboI	GATC	9 cut(s)	367, 427, 535, 838, 1324, 1482, 1993, 2382, 2470
MboII	GAAGA	9 cut(s)	105, 119, 243, 303, 539, 1234, 1334, 2392, 2416
MflI	RGATCY	1 cut(s)	1324
MhlI	GDGCHC	5 cut(s)	997, 1358, 1494, 1555, 2353
MlsI	TGGCCA	2 cut(s)	455, 1846
MluCI	AATT	9 cut(s)	240, 253, 259, 326, 409, 813, 999, 1558, 2478
MluNI	TGGCCA	2 cut(s)	455, 1846
Mly113I	GGCGCC	1 cut(s)	471
MlyI	GAGTC	1 cut(s)	455
MmeI	TCCRAC	5 cut(s)	669, 898, 1313, 1460, 1741
Mox20I	TGGCCA	2 cut(s)	455, 1846
Mph1103I	ATGCAT	1 cut(s)	1432
MroI	TCCGGA	2 cut(s)	729, 2280
MroXI	GAANNNNTTC	1 cut(s)	481
MscI	TGGCCA	2 cut(s)	455, 1846
MslI	CAYNNNNRTG	3 cut(s)	962, 1497, 1741
Msp20I	TGGCCA	2 cut(s)	455, 1846
MspA1I	CMGCKG	1 cut(s)	1469
MspI	CCGG	8 cut(s)	149, 474, 633, 730, 1029, 1835, 2281, 2333
MspR9I	CCNGG	9 cut(s)	82, 150, 1572, 1836, 1843, 1857, 1968, 2034, 2355
Mva1269I	GAATGC	1 cut(s)	1937
MvaI	CCWGG	7 cut(s)	82, 1572, 1843, 1857, 1968, 2034, 2355
MvnI	CGCG	1 cut(s)	1684
MwoI	GCNNNNNNNGC	3 cut(s)	774, 1852, 2189
NarI	GGCGCC	1 cut(s)	471
NciI	CCSGG	2 cut(s)	150, 1836
NdeII	GATC	9 cut(s)	367, 427, 535, 838, 1324, 1482, 1993, 2382, 2470
NmuCI	GTSAC	2 cut(s)	67, 1501
NruI	TCGCGA	1 cut(s)	1684
NsiI	ATGCAT	1 cut(s)	1432
NspI	RCATGY	2 cut(s)	168, 443
PagI	TCATGA	3 cut(s)	1818, 1996, 2385
PaqCI	CACCTGC	1 cut(s)	441
PasI	CCCWGGG	1 cut(s)	2033
PcsI	WCGNNNNNNNCGW	1 cut(s)	1681
PctI	GAATGC	1 cut(s)	1937
PdmI	GAANNNNTTC	1 cut(s)	481
PfeI	GAWTC	9 cut(s)	175, 248, 353, 512, 703, 1043, 1766, 2153, 2395
PkrI	GCNGC	7 cut(s)	376, 438, 1150, 1411, 2063, 2191, 2329
PleI	GAGTC	1 cut(s)	454
PluTI	GGCGCC	1 cut(s)	474
PpsI	GAGTC	1 cut(s)	454
PpuMI	RGGWCCY	2 cut(s)	625, 1699
PshBI	ATTAAT	4 cut(s)	45, 51, 57, 1725
PsiI	TTATAA	1 cut(s)	1157
Psp1406I	AACGTT	2 cut(s)	885, 2118
Psp5II	RGGWCCY	2 cut(s)	625, 1699
Psp6I	CCWGG	7 cut(s)	80, 1570, 1841, 1855, 1966, 2032, 2353
PspFI	CCCAGC	2 cut(s)	548, 870
PspGI	CCWGG	7 cut(s)	80, 1570, 1841, 1855, 1966, 2032, 2353
PspOMI	GGGCCC	1 cut(s)	2349
PspPPI	RGGWCCY	2 cut(s)	625, 1699
PstI	CTGCAG	1 cut(s)	1042
PstNI	CAGNNNCTG	1 cut(s)	598
PsuI	RGATCY	1 cut(s)	1324
PvuII	CAGCTG	1 cut(s)	1469
RruI	TCGCGA	1 cut(s)	1684
RsaI	GTAC	7 cut(s)	79, 1234, 1406, 1465, 1988, 2140, 2262
RsaNI	GTAC	7 cut(s)	78, 1233, 1405, 1464, 1987, 2139, 2261
RseI	CAYNNNNRTG	3 cut(s)	962, 1497, 1741
SatI	GCNGC	7 cut(s)	375, 437, 1149, 1410, 2062, 2190, 2328
Sau3AI	GATC	9 cut(s)	367, 427, 535, 838, 1324, 1482, 1993, 2382, 2470
ScaI	AGTACT	1 cut(s)	1234
SchI	GAGTC	1 cut(s)	455
ScrFI	CCNGG	9 cut(s)	82, 150, 1572, 1836, 1843, 1857, 1968, 2034, 2355
SduI	GDGCHC	5 cut(s)	997, 1358, 1494, 1555, 2353
SfaNI	GCATC	6 cut(s)	25, 841, 925, 1135, 1306, 2048
SfcI	CTRYAG	2 cut(s)	1038, 1182
SfoI	GGCGCC	1 cut(s)	472
SinI	GGWCC	8 cut(s)	4, 615, 625, 642, 1511, 1641, 1699, 2318
SmiI	ATTTAAAT	1 cut(s)	331
SmiMI	CAYNNNNRTG	3 cut(s)	962, 1497, 1741
Sse9I	AATT	9 cut(s)	240, 253, 259, 326, 409, 813, 999, 1558, 2478
SsiI	CCGC	9 cut(s)	216, 339, 436, 630, 750, 1173, 1319, 1639, 2453
SspDI	GGCGCC	1 cut(s)	470
SspI	AATATT	3 cut(s)	43, 49, 55
SspMI	CTAG	2 cut(s)	2415, 2497
StyD4I	CCNGG	9 cut(s)	80, 148, 1570, 1834, 1841, 1855, 1966, 2032, 2353
StyI	CCWWGG	1 cut(s)	1702
SwaI	ATTTAAAT	1 cut(s)	331
TaaI	ACNGT	7 cut(s)	67, 594, 615, 1475, 1991, 2270, 2421
TaiI	ACGT	3 cut(s)	579, 888, 2121
TaqI	TCGA	5 cut(s)	449, 621, 708, 997, 2469
TasI	AATT	9 cut(s)	240, 253, 259, 326, 409, 813, 999, 1558, 2478
TatI	WGTACW	2 cut(s)	1232, 1463
TauI	GCSGC	1 cut(s)	439
TfiI	GAWTC	9 cut(s)	175, 248, 353, 512, 703, 1043, 1766, 2153, 2395
TseFI	GTSAC	2 cut(s)	67, 1501
TseI	GCWGC	6 cut(s)	374, 1148, 1409, 2061, 2189, 2327
Tsp45I	GTSAC	2 cut(s)	67, 1501
TspDTI	ATGAA	7 cut(s)	119, 167, 1235, 1560, 1807, 1920, 2013
TspGWI	ACGGA	2 cut(s)	183, 199
VpaK11BI	GGWCC	8 cut(s)	4, 615, 625, 642, 1511, 1641, 1699, 2318
VspI	ATTAAT	4 cut(s)	45, 51, 57, 1725
XapI	RAATTY	2 cut(s)	326, 409
XbaI	TCTAGA	1 cut(s)	2414
XceI	RCATGY	2 cut(s)	168, 443
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2351
XmiI	GTMKAC	1 cut(s)	693
XmnI	GAANNNNTTC	1 cut(s)	481
XspI	CTAG	2 cut(s)	2415, 2497
ZrmI	AGTACT	1 cut(s)	1234
Zsp2I	ATGCAT	1 cut(s)	1432

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.