Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0002023

Physical Location & Seq

Genomic Coordinates Forward (+)

81842241 .. 81849951

Gene Structure 7711 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG650500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGGCCCTTCAATATAACAATAATTATTCTAGTCATTATAGTCTTGTACATGTTGGCAAGGGATTTGCATATCGTACCAAGCCGCCGGTAGGCATAATAGTATCGTCGAGGCGTTCGGTGCTGAATCGAAGAAAATCAAGTCCAATTTGGTTTCGTCGGGCATCATCGAGATTTGAGTCCTATATAGTCGGGCACAGTCTCAGCAGCAGTCAAAAGGTGATCTGGAATAGGGAAATGGCCCATAATCTGATCACAACTAATCACCTGCGGCATGTAGAGTCGATGGCCACAATGCCTTCTGGCGCCGGAAACATTCCCCACTTGAACGCAATCATTCTGGGTGAAGCACTCGCTTCTGAAGAGAATGATCTCATCTTTCCCAGCGACGACTTCTCCCGCCAGGCTCACGTCCCCTCTCCCCAGAAGTATCTGGAGATGTATAAAAGGTCCATCGAGGACCCCGCCGGATTTTGGTCGGATTTCGCTTCCGACTTCTTTTGGAAACAGAGATGGGGCGAACAAGTCTACTTCGAGAATCTCGATGTTAGCAAAGGCGATATCAGGATTGAGTGGTTCAAGGGCGGTCTCACCAATATCTGCTACAATTGCCTTGATAGAAATGTTGAAGCTGGACTTGGTGACCGAATTGCCTTGTACTGGGAAGGCAATGAGCCCTGTCTTGATGGCTCTTTAACTTACATTCAGCTTCTACAAAACGTTTGCCAGCTTGCAAACTACTTGAAAGATATTGGAGTGAAAAAGGGCGATGCTGTTGTGGTTTACTTACCCATGTTAATGGAACTTCCAATCACAATGCTTGCCTGTGCTCGCATTGGTGCTGTTCACTCGGTTGTATTTGCTGGATTTTCTGTCGAATCTCTTGCTCAAAGAGTAGTAGATTGCAAGCCGAAAGTTGTGATCACTTGCAATGCTGTTAAAAGGGGTTCTAAGGTTATCCACCTCAAAGATATAGTTGATGCTGCCCTTATCGAATCAGCCAAAAGTGGGGTTTGTGTAGATGTATGCTTAACTTATGAGAATGAATCTGCTTTGAAGAGGGGAAGTACTAAATGGGAAGAAGGAAGAGATATATGGTGGCAGGATGTCATACCTAAATATCCAACTACTTGTGCAGTGGAGTGGGTTGATGCAGAGGATCCACTTTTTCTGCTCTATACCAGTGGGAGCACTGGGAAGCCCAAGATGACTCCATTACCTGGTGCCTGGCCACAGAAGCCTGGTTCTGCCACTTTCCCCTTTTTTGGAGTTCAGGCGGTCATTGTGGATGAGAAGGGCGTTGAAATTGAAGGCGAGTGCAGTGGTTATCTGTGTATAAAAAGCTCATGGCCTGGAGCATTCCGAACTCTTTATGGTGATCATGAAAGATATGAAACAACTTACTTTAAACCTTTCTCTGGGTATTATTTTAGTGGTGATGGATGCAGCAGGGACAAAGATGGATATCACTGGCTTACAGGAAGGGTTGATGATGTCATCAATGTTAGTGGACATCGTATCGGTACCGCAGAAGTTGAATCTGCTCTTGTATCGCATCCTCAGTGTGCAGAAGCTGCTGTGGTTGGTGTAGAGCATGAGGTTAAGGGACAAGGTATATATGCCTTTGTTACTCTTGTGGACGGAGTACCTTACAGTGAAGATCTCCGGAAAAGCCTTATTCTTATTGTCCGAAAGCAGATAGGGGCATTTGCAGCACCGGACAGAATCCACTGGGCACCTGGCCTTCCAAAGACAAGGACACACTTGCAGATCCAAATGTGGTCGATCAACTTATTGCTCTCGCAGATACGTGATGCGAAGGTGCTAGTGGTGCTGACAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003987 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006082 GO:0006083 GO:0008150 GO:0008152 GO:0009507 GO:0009514 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017144 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00010 ko00620 ko00640 ko00680 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00620 map00640 map00680 map00720 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

ACAS_N AMP-binding AMP-binding_C

Protein Analysis

613

Amino Acids

68.56

Weight (kDa)

6.79

Isoelectric Point (pI)

36.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACAS_N	PF16177	143 - 203	1.2e-17	Acetyl-coenzyme A synthetase N-terminus
AMP-binding	PF00501	213 - 402	6.8e-38	AMP-binding enzyme
AMP-binding_C	PF13193	510 - 586	1.8e-22	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G36880 AT5G36880 AT5G36880 AT5G36880 AT5G36880 AT5G36880
fragaria_vesca	FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480
malus_domestica	MD09G1000400.v1.1 MD17G1003300.v1.1
prunus_persica	Prupe.3G315800_v2.0.a1
pyrus_communis	pycom111g00030 pycom17g00010
rosa_chinensis	RchiOBHm_Chr2g0176771
rosa_multiflora	Rmu_sc0020992.1_g000001
rosa_roxburghii	Rroxscaffold_2G00076170
rosa_rugosa	Rorug02G0598400 Rorug02G0598500 Rorug02G0598600 Rorug02G0598700 Rorug02G0598800 Rorug02G0598900 Rorug02G0599000 Rorug02G0599100 Rorug02G0599200
rosa_samantha	Rh2AG676000 Rh2BG687100 Rh2CG650500
rosa_wichuraiana	Rw2G055470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	273
Acc36I	ACCTGC	1 cut(s)	273
Acc65I	GGTACC	1 cut(s)	1520
AccB1I	GGYRCC	4 cut(s)	302, 1220, 1520, 1732
AccB7I	CCANNNNNTGG	1 cut(s)	1217
AccI	GTMKAC	1 cut(s)	525
AccIII	TCCGGA	1 cut(s)	1662
AciI	CCGC	7 cut(s)	83, 268, 397, 462, 582, 1274, 1524
AclI	AACGTT	1 cut(s)	717
AclWI	GGATC	3 cut(s)	1151, 1164, 1762
AcoI	YGGCCR	2 cut(s)	285, 1226
AcuI	CTGAAG	1 cut(s)	378
AcyI	GRCGYC	1 cut(s)	303
AfaI	GTAC	6 cut(s)	48, 76, 656, 1066, 1522, 1644
AfiI	CCNNNNNNNGG	7 cut(s)	89, 471, 657, 1005, 1217, 1262, 1415
AflIII	ACRYGT	1 cut(s)	49
AgsI	TTSAA	9 cut(s)	11, 325, 577, 626, 742, 1054, 1301, 1307, 1535
AjiI	CACGTC	1 cut(s)	409
AjnI	CCWGG	6 cut(s)	399, 1216, 1223, 1237, 1348, 1735
AjuI	GAANNNNNNNTTGG	2 cut(s)	618, 650
AluBI	AGCT	5 cut(s)	629, 706, 727, 1341, 1571
AluI	AGCT	5 cut(s)	629, 706, 727, 1341, 1571
Alw21I	GWGCWC	2 cut(s)	829, 1190
Alw26I	GTCTC	2 cut(s)	203, 590
AlwI	GGATC	3 cut(s)	1151, 1164, 1762
AlwNI	CAGNNNCTG	1 cut(s)	1571
Aor13HI	TCCGGA	1 cut(s)	1662
AoxI	GGCC	6 cut(s)	3, 237, 285, 1226, 1346, 1738
ApeKI	GCWGC	5 cut(s)	204, 980, 1443, 1571, 1709
Asp718I	GGTACC	1 cut(s)	1520
AspLEI	GCGC	1 cut(s)	305
AspS9I	GGNCC	4 cut(s)	4, 238, 447, 457
AsuHPI	GGTGA	7 cut(s)	229, 254, 353, 580, 650, 1385, 1445
AvaII	GGWCC	2 cut(s)	447, 457
BaeGI	GKGCMC	2 cut(s)	195, 1735
BalI	TGGCCA	2 cut(s)	287, 1228
BamHI	GGATCC	1 cut(s)	1156
BanI	GGYRCC	4 cut(s)	302, 1220, 1520, 1732
BanII	GRGCYC	1 cut(s)	675
Bbv12I	GWGCWC	2 cut(s)	829, 1190
BbvI	GCAGC	5 cut(s)	216, 967, 1455, 1558, 1721
BccI	CCATC	6 cut(s)	277, 458, 504, 677, 1430, 1451
BcgI	CGANNNNNNTGC	2 cut(s)	863, 897
BciT130I	CCWGG	6 cut(s)	401, 1218, 1225, 1239, 1350, 1737
BclI	TGATCA	3 cut(s)	249, 918, 1375
BcoDI	GTCTC	2 cut(s)	203, 590
BfaI	CTAG	2 cut(s)	30, 1823
BfoI	RGCGCY	1 cut(s)	306
BfuAI	ACCTGC	1 cut(s)	273
BglII	AGATCT	1 cut(s)	1657
BisI	GCNGC	7 cut(s)	83, 205, 269, 981, 1444, 1572, 1710
BlsI	GCNGC	7 cut(s)	84, 206, 270, 982, 1445, 1573, 1711
BmcAI	AGTACT	1 cut(s)	1066
Bme1390I	CCNGG	6 cut(s)	401, 1218, 1225, 1239, 1350, 1737
Bme18I	GGWCC	2 cut(s)	447, 457
BmgBI	CACGTC	1 cut(s)	409
BmgT120I	GGNCC	4 cut(s)	4, 238, 447, 457
BmiI	GGNNCC	6 cut(s)	304, 459, 1158, 1222, 1522, 1734
BmrFI	CCNGG	6 cut(s)	401, 1218, 1225, 1239, 1350, 1737
BmrI	ACTGGG	3 cut(s)	667, 1200, 1738
BmsI	GCATC	7 cut(s)	170, 757, 967, 1138, 1430, 1561, 1801
BmuI	ACTGGG	3 cut(s)	667, 1200, 1738
BpmI	CTGGAG	2 cut(s)	452, 1371
BsaAI	YACGTR	1 cut(s)	1808
BsaBI	GATNNNNATC	1 cut(s)	1461
BsaHI	GRCGYC	1 cut(s)	303
BsaI	GGTCTC	1 cut(s)	590
BsaWI	WCCGGW	2 cut(s)	1662, 1714
BsaXI	ACNNNNNCTCC	2 cut(s)	377, 407
Bsc4I	CCNNNNNNNGG	7 cut(s)	89, 471, 657, 1005, 1217, 1262, 1415
Bse118I	RCCGGY	1 cut(s)	85
Bse1I	ACTGG	5 cut(s)	662, 1179, 1195, 1472, 1733
Bse3DI	GCAATG	2 cut(s)	673, 934
Bse8I	GATNNNNATC	1 cut(s)	1461
BseAI	TCCGGA	1 cut(s)	1662
BseBI	CCWGG	6 cut(s)	401, 1218, 1225, 1239, 1350, 1737
BseGI	GGATG	4 cut(s)	1108, 1291, 1445, 1552
BseJI	GATNNNNATC	1 cut(s)	1461
BseLI	CCNNNNNNNGG	7 cut(s)	89, 471, 657, 1005, 1217, 1262, 1415
BseMI	GCAATG	2 cut(s)	673, 934
BseMII	CTCAG	2 cut(s)	214, 1571
BseNI	ACTGG	5 cut(s)	662, 1179, 1195, 1472, 1733
BseSI	GKGCMC	2 cut(s)	195, 1735
BseXI	GCAGC	5 cut(s)	216, 967, 1455, 1558, 1721
BseYI	CCCAGC	1 cut(s)	380
BsgI	GTGCAG	3 cut(s)	1152, 1336, 1584
BshFI	GGCC	6 cut(s)	5, 239, 287, 1228, 1348, 1740
BshNI	GGYRCC	4 cut(s)	302, 1220, 1520, 1732
BsiHKAI	GWGCWC	2 cut(s)	829, 1190
BsiSI	CCGG	5 cut(s)	86, 306, 465, 1663, 1715
BslFI	GGGAC	3 cut(s)	395, 1463, 1617
BslI	CCNNNNNNNGG	7 cut(s)	89, 471, 657, 1005, 1217, 1262, 1415
BsmAI	GTCTC	2 cut(s)	203, 590
BsmFI	GGGAC	3 cut(s)	395, 1463, 1617
BsmI	GAATGC	1 cut(s)	1355
BsnI	GGCC	6 cut(s)	5, 239, 287, 1228, 1348, 1740
Bso31I	GGTCTC	1 cut(s)	590
Bsp1286I	GDGCHC	5 cut(s)	195, 675, 829, 1190, 1735
Bsp13I	TCCGGA	1 cut(s)	1662
Bsp1407I	TGTACA	1 cut(s)	46
Bsp143I	GATC	9 cut(s)	219, 249, 367, 918, 1156, 1375, 1657, 1767, 1782
BspACI	CCGC	7 cut(s)	83, 268, 397, 462, 582, 1274, 1524
BspANI	GGCC	6 cut(s)	5, 239, 287, 1228, 1348, 1740
BspCNI	CTCAG	2 cut(s)	213, 1570
BspEI	TCCGGA	1 cut(s)	1662
BspHI	TCATGA	1 cut(s)	1378
BspLI	GGNNCC	6 cut(s)	304, 459, 1158, 1222, 1522, 1734
BspMI	ACCTGC	1 cut(s)	273
BspPI	GGATC	3 cut(s)	1151, 1164, 1762
BspT107I	GGYRCC	4 cut(s)	302, 1220, 1520, 1732
BspTNI	GGTCTC	1 cut(s)	590
BsrDI	GCAATG	2 cut(s)	673, 934
BsrFI	RCCGGY	1 cut(s)	85
BsrGI	TGTACA	1 cut(s)	46
BsrI	ACTGG	5 cut(s)	662, 1179, 1195, 1472, 1733
BssAI	RCCGGY	1 cut(s)	85
BssMI	GATC	9 cut(s)	219, 249, 367, 918, 1156, 1375, 1657, 1767, 1782
BssNI	GRCGYC	1 cut(s)	303
Bst2UI	CCWGG	6 cut(s)	401, 1218, 1225, 1239, 1350, 1737
Bst4CI	ACNGT	2 cut(s)	197, 1652
Bst6I	CTCTTC	3 cut(s)	354, 1049, 1078
BstACI	GRCGYC	1 cut(s)	303
BstAPI	GCANNNNNTGC	1 cut(s)	1571
BstAUI	TGTACA	1 cut(s)	46
BstBAI	YACGTR	1 cut(s)	1808
BstC8I	GCNNGC	5 cut(s)	725, 729, 819, 829, 905
BstDEI	CTNAG	3 cut(s)	200, 948, 1557
BstEII	GGTNACC	1 cut(s)	638
BstF5I	GGATG	4 cut(s)	1108, 1291, 1445, 1552
BstH2I	RGCGCY	1 cut(s)	306
BstHHI	GCGC	1 cut(s)	305
BstKTI	GATC	9 cut(s)	222, 252, 370, 921, 1159, 1378, 1660, 1770, 1785
BstMAI	GTCTC	2 cut(s)	203, 590
BstMBI	GATC	9 cut(s)	219, 249, 367, 918, 1156, 1375, 1657, 1767, 1782
BstMWI	GCNNNNNNNGC	6 cut(s)	118, 606, 1234, 1571, 1709, 1828
BstNI	CCWGG	6 cut(s)	401, 1218, 1225, 1239, 1350, 1737
BstNSI	RCATGY	2 cut(s)	53, 275
BstPI	GGTNACC	1 cut(s)	638
BstSCI	CCNGG	6 cut(s)	399, 1216, 1223, 1237, 1348, 1735
BstSLI	GKGCMC	2 cut(s)	195, 1735
BstV1I	GCAGC	5 cut(s)	216, 967, 1455, 1558, 1721
BstX2I	RGATCY	3 cut(s)	1156, 1657, 1767
BstXI	CCANNNNNNTGG	1 cut(s)	796
BstYI	RGATCY	3 cut(s)	1156, 1657, 1767
BsuRI	GGCC	6 cut(s)	5, 239, 287, 1228, 1348, 1740
BtgZI	GCGATG	1 cut(s)	780
BtrI	CACGTC	1 cut(s)	409
BtsCI	GGATG	4 cut(s)	1108, 1291, 1445, 1552
BtsI	GCAGTG	2 cut(s)	1140, 1324
BtsIMutI	CAGTG	8 cut(s)	1140, 1186, 1188, 1324, 1465, 1565, 1657, 1726
BveI	ACCTGC	1 cut(s)	273
Cac8I	GCNNGC	5 cut(s)	725, 729, 819, 829, 905
CaiI	CAGNNNCTG	1 cut(s)	1571
CciI	TCATGA	1 cut(s)	1378
CfoI	GCGC	1 cut(s)	305
Cfr10I	RCCGGY	1 cut(s)	85
Cfr13I	GGNCC	4 cut(s)	4, 238, 447, 457
CsiI	ACCWGGT	1 cut(s)	1216
Csp6I	GTAC	6 cut(s)	47, 75, 655, 1065, 1521, 1643
CspCI	CAANNNNNGTGG	2 cut(s)	1486, 1521
CviAII	CATG	6 cut(s)	50, 272, 790, 1344, 1379, 1592
CviQI	GTAC	6 cut(s)	47, 75, 655, 1065, 1521, 1643
DdeI	CTNAG	3 cut(s)	200, 948, 1557
DinI	GGCGCC	1 cut(s)	304
DpnI	GATC	9 cut(s)	221, 251, 369, 920, 1158, 1377, 1659, 1769, 1784
DpnII	GATC	9 cut(s)	219, 249, 367, 918, 1156, 1375, 1657, 1767, 1782
DraI	TTTAAA	1 cut(s)	1405
EaeI	YGGCCR	2 cut(s)	285, 1226
Eam1104I	CTCTTC	3 cut(s)	354, 1049, 1078
EarI	CTCTTC	3 cut(s)	354, 1049, 1078
Eco24I	GRGCYC	1 cut(s)	675
Eco31I	GGTCTC	1 cut(s)	590
Eco32I	GATATC	2 cut(s)	559, 1463
Eco47I	GGWCC	2 cut(s)	447, 457
Eco57I	CTGAAG	1 cut(s)	378
Eco91I	GGTNACC	1 cut(s)	638
EcoO109I	RGGNCCY	1 cut(s)	457
EcoO65I	GGTNACC	1 cut(s)	638
EcoRII	CCWGG	6 cut(s)	399, 1216, 1223, 1237, 1348, 1735
EcoRV	GATATC	2 cut(s)	559, 1463
EcoT38I	GRGCYC	1 cut(s)	675
EgeI	GGCGCC	1 cut(s)	304
EheI	GGCGCC	1 cut(s)	304
FaeI	CATG	6 cut(s)	53, 275, 793, 1347, 1382, 1595
FalI	AAGNNNNNCTT	2 cut(s)	618, 650
FaqI	GGGAC	3 cut(s)	395, 1463, 1617
FatI	CATG	6 cut(s)	49, 271, 789, 1343, 1378, 1591
FauI	CCCGC	2 cut(s)	404, 469
FbaI	TGATCA	3 cut(s)	249, 918, 1375
FblI	GTMKAC	1 cut(s)	525
Fnu4HI	GCNGC	7 cut(s)	83, 205, 269, 981, 1444, 1572, 1710
FokI	GGATG	4 cut(s)	1115, 1298, 1452, 1539
FriOI	GRGCYC	1 cut(s)	675
Fsp4HI	GCNGC	7 cut(s)	83, 205, 269, 981, 1444, 1572, 1710
FspBI	CTAG	2 cut(s)	30, 1823
GlaI	GCGC	1 cut(s)	304
GluI	GCNGC	7 cut(s)	83, 205, 269, 981, 1444, 1572, 1710
GsaI	CCCAGC	1 cut(s)	384
GsuI	CTGGAG	2 cut(s)	452, 1371
HaeII	RGCGCY	1 cut(s)	306
HaeIII	GGCC	6 cut(s)	5, 239, 287, 1228, 1348, 1740
HapII	CCGG	5 cut(s)	86, 306, 465, 1663, 1715
HhaI	GCGC	1 cut(s)	305
Hin1I	GRCGYC	1 cut(s)	303
Hin1II	CATG	6 cut(s)	53, 275, 793, 1347, 1382, 1595
Hin6I	GCGC	1 cut(s)	303
HinP1I	GCGC	1 cut(s)	303
HpaII	CCGG	5 cut(s)	86, 306, 465, 1663, 1715
HphI	GGTGA	7 cut(s)	229, 254, 353, 580, 650, 1385, 1445
Hpy166II	GTNNAC	5 cut(s)	526, 781, 844, 1508, 1636
Hpy188I	TCNGA	6 cut(s)	249, 358, 478, 490, 1361, 1688
Hpy188III	TCNNGA	9 cut(s)	168, 223, 431, 532, 539, 562, 680, 1379, 1663
Hpy8I	GTNNAC	5 cut(s)	526, 781, 844, 1508, 1636
Hpy99I	CGWCG	3 cut(s)	109, 159, 389
HpyAV	CCTTC	9 cut(s)	17, 306, 656, 1073, 1285, 1301, 1473, 1751, 1810
HpyCH4III	ACNGT	2 cut(s)	197, 1652
HpyCH4IV	ACGT	3 cut(s)	408, 717, 1807
HpyF10VI	GCNNNNNNNGC	6 cut(s)	118, 606, 1234, 1571, 1709, 1828
HpyF3I	CTNAG	3 cut(s)	200, 948, 1557
HpySE526I	ACGT	3 cut(s)	408, 717, 1807
Hsp92I	GRCGYC	1 cut(s)	303
Hsp92II	CATG	6 cut(s)	53, 275, 793, 1347, 1382, 1595
HspAI	GCGC	1 cut(s)	303
KasI	GGCGCC	1 cut(s)	302
Kpn2I	TCCGGA	1 cut(s)	1662
KpnI	GGTACC	1 cut(s)	1524
Ksp22I	TGATCA	3 cut(s)	249, 918, 1375
Kzo9I	GATC	9 cut(s)	219, 249, 367, 918, 1156, 1375, 1657, 1767, 1782
LmnI	GCTCC	2 cut(s)	1185, 1352
Lsp1109I	GCAGC	5 cut(s)	216, 967, 1455, 1558, 1721
LweI	GCATC	7 cut(s)	170, 757, 967, 1138, 1430, 1561, 1801
MabI	ACCWGGT	1 cut(s)	1216
MaeI	CTAG	2 cut(s)	30, 1823
MaeII	ACGT	3 cut(s)	408, 717, 1807
MaeIII	GTNAC	2 cut(s)	638, 1624
MalI	GATC	9 cut(s)	221, 251, 369, 920, 1158, 1377, 1659, 1769, 1784
MboI	GATC	9 cut(s)	219, 249, 367, 918, 1156, 1375, 1657, 1767, 1782
MboII	GAAGA	6 cut(s)	141, 371, 1066, 1088, 1095, 1667
MfeI	CAATTG	1 cut(s)	604
MflI	RGATCY	3 cut(s)	1156, 1657, 1767
MhlI	GDGCHC	5 cut(s)	195, 675, 829, 1190, 1735
MlsI	TGGCCA	2 cut(s)	287, 1228
MluCI	AATT	5 cut(s)	22, 144, 604, 645, 1302
MluNI	TGGCCA	2 cut(s)	287, 1228
Mly113I	GGCGCC	1 cut(s)	303
MlyI	GAGTC	3 cut(s)	185, 287, 1201
MmeI	TCCRAC	3 cut(s)	456, 513, 1145
MnlI	CCTC	8 cut(s)	102, 424, 448, 971, 1050, 1147, 1566, 1588
Mox20I	TGGCCA	2 cut(s)	287, 1228
MroI	TCCGGA	1 cut(s)	1662
MscI	TGGCCA	2 cut(s)	287, 1228
MseI	TTAA	6 cut(s)	692, 794, 936, 1028, 1404, 1599
MslI	CAYNNNNRTG	1 cut(s)	794
Msp20I	TGGCCA	2 cut(s)	287, 1228
MspI	CCGG	5 cut(s)	86, 306, 465, 1663, 1715
MspR9I	CCNGG	6 cut(s)	401, 1218, 1225, 1239, 1350, 1737
MunI	CAATTG	1 cut(s)	604
Mva1269I	GAATGC	1 cut(s)	1355
MvaI	CCWGG	6 cut(s)	401, 1218, 1225, 1239, 1350, 1737
MwoI	GCNNNNNNNGC	6 cut(s)	118, 606, 1234, 1571, 1709, 1828
NarI	GGCGCC	1 cut(s)	303
NdeII	GATC	9 cut(s)	219, 249, 367, 918, 1156, 1375, 1657, 1767, 1782
NlaIII	CATG	6 cut(s)	53, 275, 793, 1347, 1382, 1595
NlaIV	GGNNCC	6 cut(s)	304, 459, 1158, 1222, 1522, 1734
NmuCI	GTSAC	1 cut(s)	638
NspI	RCATGY	2 cut(s)	53, 275
PagI	TCATGA	1 cut(s)	1378
PaqCI	CACCTGC	1 cut(s)	273
PciI	ACATGT	1 cut(s)	49
PcsI	WCGNNNNNNNCGW	1 cut(s)	537
PctI	GAATGC	1 cut(s)	1355
PfeI	GAWTC	7 cut(s)	124, 535, 875, 992, 1043, 1535, 1722
PflMI	CCANNNNNTGG	1 cut(s)	1217
PkrI	GCNGC	7 cut(s)	84, 206, 270, 982, 1445, 1573, 1711
PleI	GAGTC	3 cut(s)	184, 286, 1201
PluTI	GGCGCC	1 cut(s)	306
PpsI	GAGTC	3 cut(s)	184, 286, 1201
Ppu21I	YACGTR	1 cut(s)	1808
PpuMI	RGGWCCY	1 cut(s)	457
PscI	ACATGT	1 cut(s)	49
Psp1406I	AACGTT	1 cut(s)	717
Psp5II	RGGWCCY	1 cut(s)	457
Psp6I	CCWGG	6 cut(s)	399, 1216, 1223, 1237, 1348, 1735
PspEI	GGTNACC	1 cut(s)	638
PspFI	CCCAGC	1 cut(s)	380
PspGI	CCWGG	6 cut(s)	399, 1216, 1223, 1237, 1348, 1735
PspN4I	GGNNCC	6 cut(s)	304, 459, 1158, 1222, 1522, 1734
PspPI	GGNCC	4 cut(s)	4, 238, 447, 457
PspPPI	RGGWCCY	1 cut(s)	457
PstNI	CAGNNNCTG	1 cut(s)	1571
PsuI	RGATCY	3 cut(s)	1156, 1657, 1767
RsaI	GTAC	6 cut(s)	48, 76, 656, 1066, 1522, 1644
RsaNI	GTAC	6 cut(s)	47, 75, 655, 1065, 1521, 1643
RseI	CAYNNNNRTG	1 cut(s)	794
SaqAI	TTAA	6 cut(s)	692, 794, 936, 1028, 1404, 1599
SatI	GCNGC	7 cut(s)	83, 205, 269, 981, 1444, 1572, 1710
Sau3AI	GATC	9 cut(s)	219, 249, 367, 918, 1156, 1375, 1657, 1767, 1782
Sau96I	GGNCC	4 cut(s)	4, 238, 447, 457
ScaI	AGTACT	1 cut(s)	1066
SchI	GAGTC	3 cut(s)	185, 287, 1201
ScrFI	CCNGG	6 cut(s)	401, 1218, 1225, 1239, 1350, 1737
SduI	GDGCHC	5 cut(s)	195, 675, 829, 1190, 1735
SexAI	ACCWGGT	1 cut(s)	1216
SfaNI	GCATC	7 cut(s)	170, 757, 967, 1138, 1430, 1561, 1801
SfoI	GGCGCC	1 cut(s)	304
SinI	GGWCC	2 cut(s)	447, 457
SmiMI	CAYNNNNRTG	1 cut(s)	794
Sse9I	AATT	5 cut(s)	22, 144, 604, 645, 1302
SsiI	CCGC	7 cut(s)	83, 268, 397, 462, 582, 1274, 1524
SspDI	GGCGCC	1 cut(s)	302
SspMI	CTAG	2 cut(s)	30, 1823
StyD4I	CCNGG	6 cut(s)	399, 1216, 1223, 1237, 1348, 1735
TaaI	ACNGT	2 cut(s)	197, 1652
TaiI	ACGT	3 cut(s)	411, 720, 1810
TaqII	GACCGA	1 cut(s)	657
TasI	AATT	5 cut(s)	22, 144, 604, 645, 1302
TatI	WGTACW	3 cut(s)	46, 654, 1064
TauI	GCSGC	2 cut(s)	85, 271
TfiI	GAWTC	7 cut(s)	124, 535, 875, 992, 1043, 1535, 1722
Tru1I	TTAA	6 cut(s)	692, 794, 936, 1028, 1404, 1599
Tru9I	TTAA	6 cut(s)	692, 794, 936, 1028, 1404, 1599
TscAI	CASTG	8 cut(s)	1140, 1186, 1195, 1324, 1472, 1565, 1657, 1733
TseFI	GTSAC	1 cut(s)	638
TseI	GCWGC	5 cut(s)	204, 980, 1443, 1571, 1709
Tsp45I	GTSAC	1 cut(s)	638
TspDTI	ATGAA	3 cut(s)	1056, 1395, 1404
TspGWI	ACGGA	1 cut(s)	1653
TspRI	CASTG	8 cut(s)	1140, 1186, 1195, 1324, 1472, 1565, 1657, 1733
Van91I	CCANNNNNTGG	1 cut(s)	1217
VpaK11BI	GGWCC	2 cut(s)	447, 457
XceI	RCATGY	2 cut(s)	53, 275
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1733
XmiI	GTMKAC	1 cut(s)	525
XspI	CTAG	2 cut(s)	30, 1823
ZrmI	AGTACT	1 cut(s)	1066

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.