Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002023

Physical Location & Seq

Genomic Coordinates Reverse (-)

90992 .. 102262

Gene Structure 11271 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00076170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2331 bp

ATGGCCTCTCAATATAACAATAATTATTCTAGTCATTATAGTCTTGAACATGTTGGCAAGGGATTTGCGTATCGTACCAAGCCGGCGGTAGGCATAATAGTATCGTCGTGGCGTTCGGTGCTGAATCGAAGAAAATCAAGTCCAATTTCGTTTCGTCGGGCATCGTCGAGATTTGAGTCCTTCATAGTCGGGCACAGTCTTAGCAGCGGTCAAAAGGTGATCTGGAATAGGGAAATGGCCCATAATCTGATTACAACTAATCACCTGCGGCATGTAGAGTCGATGGCCACAATGCCTTCTGGCGCCGGAAACATTCCCCACTTGAACGCAATCATTCTGGGTGAAGCACTCGCTTCTGAAGAGAATGATCTCATCTTTCCCAGCGACGAATTCTCCCGCCAGGCTCATGTCCCCTCTCCCCAGAAGTATCTGGAGATGTATAAAAGGTCCATTGAGGACCCCGCCGGATTTTGGTCGGATTTTGCTTCCGACTTCTTTTGGAAACAGAGATGGGGCGAACAAGTCTACTTCGAGAATCTCGATGTTAGCAAAGGCGATATCAGGATTGAGTGGTTCAAGGGCGGTCTCACCAATATCTGCTACAATTGCCTTGATAGAAATGTTGAAGCTGGACTTGGTGACCGAATTGCCTTGTACTGGGAAGGCAATGAGCCCTGTCTTGATGGCTCTTTAACTTACATTCAGCTTCTACAAAACGTTTGCCAGCTTGCAAACTACTTGAAAGATATTGGAGTGAAAAAGGGCGATGCTGTTGTGGTTTACTTACCCATGTTAATGGAACTTCCAATCACAATGCTTGCCTGTGCTCGCATTGGTGCTGTTCACTCGGTTGTATTTGCTGGATTTTCTGTCGAATCTCTTGCTCAAAGAGTAGTAGATTGCAAGCCAAAAGTTGTGATCACTTGCAATGCTGTTAAAAGGGGTTCTAAGGTCATCCACCTCAAAGATATAGTTGATGCTGCCCTTATCGAATCTTCCAAAAGTGGGGTTTGTGTAGATGTATGCTTAACTTATGAGAATGAATCTGCTTTGAAGAGGGGAAGTACTAAATGGGAAGAAGGAAGAGATATATGGTGGCAGGATGTCATACCTAAATATCCAACTACTTGTGCAGTGGAGTGGGTTGATGCAGAGGATCCACTTTTTCTGCTCTATACCAGTGGGAGCACTGGGAAGCCCAAGGGAGTTCTGCACACAACTGGAGGATATATGGTGTACACTGCTACAACATTTAAATATGCTTTTGATTACAAACCATCTGACATCTACTGGTGTACAGCTGACTGTGGTTGGATCACTGGGCACAGCTATGTCACTTATGGACCTCTGCTAAATGGAGCAACTGTTGTCATATATGAAGGGGCACCAAATTATCCTGACCCTGGACGCTGTTGGGATATTGTTGATAAATACAAAGTGACCATCTTTTATACTGCCCCCACATTGGTGCGGTCTCTCATGCGTGATGGTGATGAGTATGTCAAGCGATACTCGCGAAAATCATTACGGGTCCTTGGAAGTGTAGGCGAGCCTATTAATCCAAGTGCATGGAGGTGGTTTTTCAATGTGGTTGGAGATTCAAGATGTCCCATTTCTGACACTTGGTGGCAAACTGAAACTGGTGGCTTTATGATGACTCCATTACCTGGTGCCTGGCCACAGAAGCCTGGTTCTGCCACTTTCCCCTTTTTTGGAGTTCAGGCTGTCATTGTGGATGAGAAGGGCGTTGAAATTGAAGGCGAGTGCAGTGGTTATCTGTGTATAAAAAGCTCATGGCCTGGAGCATTCCGAACTCTTTATGGTGATCATGAAAGATATGAAACAACTTACTTTAAACCTTTCTCTGGGTATTATTTTAGCGGTGATGGATGCAGCAGGGACAAAGATGGATATCACTGGCTTACAGGAAGGGTTGATGATGTCATCAATGTTAGTGGACATCGTATCGGTACCGCAGAAGTTGAATCTGCTCTTGTATCGCATCCTCAGTGTGCAGAAGCTGCTGTGGTTGGTGTAGAGCATGAGGTTAAAGGACAAGGTATATATGCCTTTGTTACTCTTGTGGACGGAGTACCTTACAGTGAAGATCTCCGGAAAAGCCTTATTCTTATTGTCAGAAAGCAGATAGGGGCATTTGCAGCACCGGACAGAATCCACTGGGCACCTGGCCTTCCAAAGACAAGAAGTGGGAAGATAATGAGGAGAATTCTGAGGAAAATTGCTTCAAAACAGTTAGACGAGCTTGGTGACACTAGCACACTTGCAGATCCAAATGTGGTCGATCAACTAATTGCTCTTGCAGATACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003987 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006082 GO:0006083 GO:0008150 GO:0008152 GO:0009507 GO:0009514 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017144 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00010 ko00620 ko00640 ko00680 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00620 map00640 map00680 map00720 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

ACAS_N AMP-binding AMP-binding_C

Protein Analysis

776

Amino Acids

86.68

Weight (kDa)

6.34

Isoelectric Point (pI)

32.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACAS_N	PF16177	143 - 203	1.6e-17	Acetyl-coenzyme A synthetase N-terminus
AMP-binding	PF00501	213 - 598	4.8e-92	AMP-binding enzyme
AMP-binding_C	PF13193	660 - 738	2.5e-24	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G36880 AT5G36880 AT5G36880 AT5G36880 AT5G36880 AT5G36880
fragaria_vesca	FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480
malus_domestica	MD09G1000400.v1.1 MD17G1003300.v1.1
prunus_persica	Prupe.3G315800_v2.0.a1
pyrus_communis	pycom111g00030 pycom17g00010
rosa_chinensis	RchiOBHm_Chr2g0176771
rosa_multiflora	Rmu_sc0020992.1_g000001
rosa_roxburghii	Rroxscaffold_2G00076170
rosa_rugosa	Rorug02G0598400 Rorug02G0598500 Rorug02G0598600 Rorug02G0598700 Rorug02G0598800 Rorug02G0598900 Rorug02G0599000 Rorug02G0599100 Rorug02G0599200
rosa_samantha	Rh2AG676000 Rh2BG687100 Rh2CG650500
rosa_wichuraiana	Rw2G055470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	273
Acc36I	ACCTGC	1 cut(s)	273
Acc65I	GGTACC	1 cut(s)	1970
AccB1I	GGYRCC	5 cut(s)	302, 1384, 1670, 1970, 2182
AccB7I	CCANNNNNTGG	1 cut(s)	1667
AccI	GTMKAC	1 cut(s)	525
AccII	CGCG	1 cut(s)	1516
AccIII	TCCGGA	1 cut(s)	2112
AciI	CCGC	9 cut(s)	86, 207, 268, 397, 462, 582, 1471, 1881, 1974
AclI	AACGTT	1 cut(s)	717
AclWI	GGATC	4 cut(s)	1151, 1164, 1322, 2282
AcoI	YGGCCR	2 cut(s)	285, 1676
AcsI	RAATTY	2 cut(s)	389, 2226
AcuI	CTGAAG	1 cut(s)	378
AcyI	GRCGYC	1 cut(s)	303
AdeI	CACNNNGTG	1 cut(s)	1626
AfaI	GTAC	7 cut(s)	76, 656, 1066, 1238, 1297, 1972, 2094
AfiI	CCNNNNNNNGG	9 cut(s)	89, 471, 657, 1005, 1403, 1465, 1667, 1712, 1865
AflIII	ACRYGT	1 cut(s)	49
AjnI	CCWGG	7 cut(s)	399, 1402, 1666, 1673, 1687, 1798, 2185
AjuI	GAANNNNNNNTTGG	2 cut(s)	618, 650
AleI	CACNNNNGTG	1 cut(s)	1466
AluBI	AGCT	8 cut(s)	629, 706, 727, 1301, 1329, 1791, 2021, 2263
AluI	AGCT	8 cut(s)	629, 706, 727, 1301, 1329, 1791, 2021, 2263
Alw21I	GWGCWC	2 cut(s)	829, 1190
Alw26I	GTCTC	2 cut(s)	590, 1479
AlwI	GGATC	4 cut(s)	1151, 1164, 1322, 2282
AlwNI	CAGNNNCTG	1 cut(s)	2021
Aor13HI	TCCGGA	1 cut(s)	2112
AoxI	GGCC	6 cut(s)	3, 237, 285, 1676, 1796, 2188
ApeKI	GCWGC	5 cut(s)	204, 980, 1893, 2021, 2159
ApoI	RAATTY	2 cut(s)	389, 2226
AseI	ATTAAT	1 cut(s)	1557
Asp718I	GGTACC	1 cut(s)	1970
AspLEI	GCGC	1 cut(s)	305
AspS9I	GGNCC	5 cut(s)	238, 447, 457, 1343, 1531
AsuHPI	GGTGA	9 cut(s)	229, 254, 353, 580, 650, 1502, 1835, 1895, 2279
AvaII	GGWCC	4 cut(s)	447, 457, 1343, 1531
BaeGI	GKGCMC	4 cut(s)	195, 1326, 1387, 2185
BalI	TGGCCA	2 cut(s)	287, 1678
BamHI	GGATCC	1 cut(s)	1156
BanI	GGYRCC	5 cut(s)	302, 1384, 1670, 1970, 2182
BanII	GRGCYC	1 cut(s)	675
Bbv12I	GWGCWC	2 cut(s)	829, 1190
BbvI	GCAGC	5 cut(s)	216, 967, 1905, 2008, 2171
BccI	CCATC	8 cut(s)	277, 504, 677, 1285, 1451, 1481, 1880, 1901
BcgI	CGANNNNNNTGC	2 cut(s)	863, 897
BciT130I	CCWGG	7 cut(s)	401, 1404, 1668, 1675, 1689, 1800, 2187
BclI	TGATCA	2 cut(s)	918, 1825
BcoDI	GTCTC	2 cut(s)	590, 1479
BfaI	CTAG	2 cut(s)	30, 2274
BfoI	RGCGCY	1 cut(s)	306
BfuAI	ACCTGC	1 cut(s)	273
BglII	AGATCT	1 cut(s)	2107
BisI	GCNGC	6 cut(s)	205, 269, 981, 1894, 2022, 2160
BlsI	GCNGC	6 cut(s)	206, 270, 982, 1895, 2023, 2161
BmcAI	AGTACT	1 cut(s)	1066
Bme1390I	CCNGG	7 cut(s)	401, 1404, 1668, 1675, 1689, 1800, 2187
Bme18I	GGWCC	4 cut(s)	447, 457, 1343, 1531
BmgT120I	GGNCC	5 cut(s)	238, 447, 457, 1343, 1531
BmiI	GGNNCC	8 cut(s)	304, 459, 1158, 1386, 1532, 1672, 1972, 2184
BmrFI	CCNGG	7 cut(s)	401, 1404, 1668, 1675, 1689, 1800, 2187
BmrI	ACTGGG	4 cut(s)	667, 1200, 1329, 2188
BmsI	GCATC	6 cut(s)	170, 757, 967, 1138, 1880, 2011
BmuI	ACTGGG	4 cut(s)	667, 1200, 1329, 2188
BpmI	CTGGAG	3 cut(s)	452, 1242, 1821
BsaAI	YACGTR	1 cut(s)	2328
BsaBI	GATNNNNATC	1 cut(s)	1911
BsaHI	GRCGYC	1 cut(s)	303
BsaI	GGTCTC	2 cut(s)	590, 1479
BsaJI	CCNNGG	3 cut(s)	1200, 1402, 1534
BsaWI	WCCGGW	2 cut(s)	2112, 2164
BsaXI	ACNNNNNCTCC	4 cut(s)	377, 407, 1350, 1380
Bsc4I	CCNNNNNNNGG	9 cut(s)	89, 471, 657, 1005, 1403, 1465, 1667, 1712, 1865
Bse118I	RCCGGY	1 cut(s)	82
Bse1I	ACTGG	9 cut(s)	662, 1179, 1195, 1225, 1295, 1324, 1645, 1922, 2183
Bse3DI	GCAATG	2 cut(s)	673, 934
Bse8I	GATNNNNATC	1 cut(s)	1911
BseAI	TCCGGA	1 cut(s)	2112
BseBI	CCWGG	7 cut(s)	401, 1404, 1668, 1675, 1689, 1800, 2187
BseDI	CCNNGG	3 cut(s)	1200, 1402, 1534
BseGI	GGATG	5 cut(s)	954, 1108, 1741, 1895, 2002
BseJI	GATNNNNATC	1 cut(s)	1911
BseLI	CCNNNNNNNGG	9 cut(s)	89, 471, 657, 1005, 1403, 1465, 1667, 1712, 1865
BseMI	GCAATG	2 cut(s)	673, 934
BseMII	CTCAG	2 cut(s)	2021, 2222
BseNI	ACTGG	9 cut(s)	662, 1179, 1195, 1225, 1295, 1324, 1645, 1922, 2183
BseRI	GAGGAG	1 cut(s)	2236
BseSI	GKGCMC	4 cut(s)	195, 1326, 1387, 2185
BseXI	GCAGC	5 cut(s)	216, 967, 1905, 2008, 2171
BseYI	CCCAGC	1 cut(s)	380
BsgI	GTGCAG	4 cut(s)	1152, 1196, 1786, 2034
Bsh1236I	CGCG	1 cut(s)	1516
BshFI	GGCC	6 cut(s)	5, 239, 287, 1678, 1798, 2190
BshNI	GGYRCC	5 cut(s)	302, 1384, 1670, 1970, 2182
BsiHKAI	GWGCWC	2 cut(s)	829, 1190
BsiSI	CCGG	5 cut(s)	83, 306, 465, 2113, 2165
BslFI	GGGAC	3 cut(s)	395, 1593, 1913
BslI	CCNNNNNNNGG	9 cut(s)	89, 471, 657, 1005, 1403, 1465, 1667, 1712, 1865
BsmAI	GTCTC	2 cut(s)	590, 1479
BsmFI	GGGAC	3 cut(s)	395, 1593, 1913
BsmI	GAATGC	1 cut(s)	1805
BsnI	GGCC	6 cut(s)	5, 239, 287, 1678, 1798, 2190
Bso31I	GGTCTC	2 cut(s)	590, 1479
Bsp1286I	GDGCHC	7 cut(s)	195, 675, 829, 1190, 1326, 1387, 2185
Bsp13I	TCCGGA	1 cut(s)	2112
Bsp1407I	TGTACA	2 cut(s)	1236, 1295
Bsp143I	GATC	9 cut(s)	219, 367, 918, 1156, 1314, 1825, 2107, 2287, 2302
Bsp68I	TCGCGA	1 cut(s)	1516
BspACI	CCGC	9 cut(s)	86, 207, 268, 397, 462, 582, 1471, 1881, 1974
BspANI	GGCC	6 cut(s)	5, 239, 287, 1678, 1798, 2190
BspCNI	CTCAG	2 cut(s)	2020, 2223
BspEI	TCCGGA	1 cut(s)	2112
BspFNI	CGCG	1 cut(s)	1516
BspHI	TCATGA	1 cut(s)	1828
BspLI	GGNNCC	8 cut(s)	304, 459, 1158, 1386, 1532, 1672, 1972, 2184
BspMI	ACCTGC	1 cut(s)	273
BspPI	GGATC	4 cut(s)	1151, 1164, 1322, 2282
BspT107I	GGYRCC	5 cut(s)	302, 1384, 1670, 1970, 2182
BspTNI	GGTCTC	2 cut(s)	590, 1479
BsrDI	GCAATG	2 cut(s)	673, 934
BsrFI	RCCGGY	1 cut(s)	82
BsrGI	TGTACA	2 cut(s)	1236, 1295
BsrI	ACTGG	9 cut(s)	662, 1179, 1195, 1225, 1295, 1324, 1645, 1922, 2183
BssAI	RCCGGY	1 cut(s)	82
BssECI	CCNNGG	3 cut(s)	1200, 1402, 1534
BssMI	GATC	9 cut(s)	219, 367, 918, 1156, 1314, 1825, 2107, 2287, 2302
BssNI	GRCGYC	1 cut(s)	303
BssT1I	CCWWGG	2 cut(s)	1200, 1534
Bst2UI	CCWGG	7 cut(s)	401, 1404, 1668, 1675, 1689, 1800, 2187
Bst4CI	ACNGT	5 cut(s)	197, 1307, 1366, 2102, 2253
Bst6I	CTCTTC	3 cut(s)	354, 1049, 1078
BstACI	GRCGYC	1 cut(s)	303
BstAPI	GCANNNNNTGC	1 cut(s)	2021
BstAUI	TGTACA	2 cut(s)	1236, 1295
BstBAI	YACGTR	1 cut(s)	2328
BstC8I	GCNNGC	7 cut(s)	84, 725, 729, 819, 829, 905, 1550
BstDEI	CTNAG	4 cut(s)	200, 948, 2007, 2231
BstEII	GGTNACC	1 cut(s)	638
BstF5I	GGATG	5 cut(s)	954, 1108, 1741, 1895, 2002
BstFNI	CGCG	1 cut(s)	1516
BstH2I	RGCGCY	1 cut(s)	306
BstHHI	GCGC	1 cut(s)	305
BstKTI	GATC	9 cut(s)	222, 370, 921, 1159, 1317, 1828, 2110, 2290, 2305
BstMAI	GTCTC	2 cut(s)	590, 1479
BstMBI	GATC	9 cut(s)	219, 367, 918, 1156, 1314, 1825, 2107, 2287, 2302
BstMWI	GCNNNNNNNGC	6 cut(s)	118, 606, 1513, 1684, 2021, 2159
BstNI	CCWGG	7 cut(s)	401, 1404, 1668, 1675, 1689, 1800, 2187
BstNSI	RCATGY	2 cut(s)	53, 275
BstPI	GGTNACC	1 cut(s)	638
BstSCI	CCNGG	7 cut(s)	399, 1402, 1666, 1673, 1687, 1798, 2185
BstSLI	GKGCMC	4 cut(s)	195, 1326, 1387, 2185
BstUI	CGCG	1 cut(s)	1516
BstV1I	GCAGC	5 cut(s)	216, 967, 1905, 2008, 2171
BstX2I	RGATCY	3 cut(s)	1156, 2107, 2287
BstXI	CCANNNNNNTGG	2 cut(s)	796, 1569
BstYI	RGATCY	3 cut(s)	1156, 2107, 2287
BsuRI	GGCC	6 cut(s)	5, 239, 287, 1678, 1798, 2190
BtgZI	GCGATG	1 cut(s)	780
BtsCI	GGATG	5 cut(s)	954, 1108, 1741, 1895, 2002
BtsI	GCAGTG	3 cut(s)	1140, 1239, 1774
BtuMI	TCGCGA	1 cut(s)	1516
BveI	ACCTGC	1 cut(s)	273
Cac8I	GCNNGC	7 cut(s)	84, 725, 729, 819, 829, 905, 1550
CaiI	CAGNNNCTG	1 cut(s)	2021
CciI	TCATGA	1 cut(s)	1828
CfoI	GCGC	1 cut(s)	305
Cfr10I	RCCGGY	1 cut(s)	82
Cfr13I	GGNCC	5 cut(s)	238, 447, 457, 1343, 1531
CseI	GACGC	1 cut(s)	1416
CsiI	ACCWGGT	1 cut(s)	1666
Csp6I	GTAC	7 cut(s)	75, 655, 1065, 1237, 1296, 1971, 2093
CspCI	CAANNNNNGTGG	2 cut(s)	1936, 1971
CviAII	CATG	9 cut(s)	50, 272, 407, 790, 1480, 1569, 1794, 1829, 2042
CviQI	GTAC	7 cut(s)	75, 655, 1065, 1237, 1296, 1971, 2093
DdeI	CTNAG	4 cut(s)	200, 948, 2007, 2231
DinI	GGCGCC	1 cut(s)	304
DpnI	GATC	9 cut(s)	221, 369, 920, 1158, 1316, 1827, 2109, 2289, 2304
DpnII	GATC	9 cut(s)	219, 367, 918, 1156, 1314, 1825, 2107, 2287, 2302
DraI	TTTAAA	2 cut(s)	1255, 1855
DraIII	CACNNNGTG	1 cut(s)	1626
EaeI	YGGCCR	2 cut(s)	285, 1676
Eam1104I	CTCTTC	3 cut(s)	354, 1049, 1078
EarI	CTCTTC	3 cut(s)	354, 1049, 1078
Eco130I	CCWWGG	2 cut(s)	1200, 1534
Eco24I	GRGCYC	1 cut(s)	675
Eco31I	GGTCTC	2 cut(s)	590, 1479
Eco32I	GATATC	2 cut(s)	559, 1913
Eco47I	GGWCC	4 cut(s)	447, 457, 1343, 1531
Eco57I	CTGAAG	1 cut(s)	378
Eco91I	GGTNACC	1 cut(s)	638
EcoO109I	RGGNCCY	2 cut(s)	457, 1531
EcoO65I	GGTNACC	1 cut(s)	638
EcoRI	GAATTC	2 cut(s)	389, 2226
EcoRII	CCWGG	7 cut(s)	399, 1402, 1666, 1673, 1687, 1798, 2185
EcoRV	GATATC	2 cut(s)	559, 1913
EcoT14I	CCWWGG	2 cut(s)	1200, 1534
EcoT38I	GRGCYC	1 cut(s)	675
EgeI	GGCGCC	1 cut(s)	304
EheI	GGCGCC	1 cut(s)	304
ErhI	CCWWGG	2 cut(s)	1200, 1534
FaeI	CATG	9 cut(s)	53, 275, 410, 793, 1483, 1572, 1797, 1832, 2045
FalI	AAGNNNNNCTT	2 cut(s)	618, 650
FaqI	GGGAC	3 cut(s)	395, 1593, 1913
FatI	CATG	9 cut(s)	49, 271, 406, 789, 1479, 1568, 1793, 1828, 2041
FauI	CCCGC	2 cut(s)	404, 469
FbaI	TGATCA	2 cut(s)	918, 1825
FblI	GTMKAC	1 cut(s)	525
Fnu4HI	GCNGC	6 cut(s)	205, 269, 981, 1894, 2022, 2160
FokI	GGATG	5 cut(s)	941, 1115, 1748, 1902, 1989
FriOI	GRGCYC	1 cut(s)	675
Fsp4HI	GCNGC	6 cut(s)	205, 269, 981, 1894, 2022, 2160
FspBI	CTAG	2 cut(s)	30, 2274
GlaI	GCGC	1 cut(s)	304
GluI	GCNGC	6 cut(s)	205, 269, 981, 1894, 2022, 2160
GsaI	CCCAGC	1 cut(s)	384
GsuI	CTGGAG	3 cut(s)	452, 1242, 1821
HaeII	RGCGCY	1 cut(s)	306
HaeIII	GGCC	6 cut(s)	5, 239, 287, 1678, 1798, 2190
HapII	CCGG	5 cut(s)	83, 306, 465, 2113, 2165
HgaI	GACGC	1 cut(s)	1416
HhaI	GCGC	1 cut(s)	305
Hin1I	GRCGYC	1 cut(s)	303
Hin1II	CATG	9 cut(s)	53, 275, 410, 793, 1483, 1572, 1797, 1832, 2045
Hin6I	GCGC	1 cut(s)	303
HinP1I	GCGC	1 cut(s)	303
HpaII	CCGG	5 cut(s)	83, 306, 465, 2113, 2165
HphI	GGTGA	9 cut(s)	229, 254, 353, 580, 650, 1502, 1835, 1895, 2279
Hpy166II	GTNNAC	8 cut(s)	526, 781, 844, 1237, 1239, 1296, 1958, 2086
Hpy188I	TCNGA	9 cut(s)	249, 358, 478, 490, 1282, 1618, 1811, 2138, 2232
Hpy8I	GTNNAC	8 cut(s)	526, 781, 844, 1237, 1239, 1296, 1958, 2086
Hpy99I	CGWCG	4 cut(s)	109, 159, 169, 389
HpyAV	CCTTC	9 cut(s)	190, 306, 656, 1073, 1373, 1735, 1751, 1923, 2201
HpyCH4III	ACNGT	5 cut(s)	197, 1307, 1366, 2102, 2253
HpyCH4IV	ACGT	2 cut(s)	717, 2327
HpyF10VI	GCNNNNNNNGC	6 cut(s)	118, 606, 1513, 1684, 2021, 2159
HpyF3I	CTNAG	4 cut(s)	200, 948, 2007, 2231
HpySE526I	ACGT	2 cut(s)	717, 2327
Hsp92I	GRCGYC	1 cut(s)	303
Hsp92II	CATG	9 cut(s)	53, 275, 410, 793, 1483, 1572, 1797, 1832, 2045
HspAI	GCGC	1 cut(s)	303
KasI	GGCGCC	1 cut(s)	302
Kpn2I	TCCGGA	1 cut(s)	2112
KpnI	GGTACC	1 cut(s)	1974
KroI	GCCGGC	1 cut(s)	82
KroNI	GCCGGC	1 cut(s)	84
Ksp22I	TGATCA	2 cut(s)	918, 1825
Kzo9I	GATC	9 cut(s)	219, 367, 918, 1156, 1314, 1825, 2107, 2287, 2302
LmnI	GCTCC	3 cut(s)	1185, 1358, 1802
Lsp1109I	GCAGC	5 cut(s)	216, 967, 1905, 2008, 2171
LweI	GCATC	6 cut(s)	170, 757, 967, 1138, 1880, 2011
MabI	ACCWGGT	1 cut(s)	1666
MaeI	CTAG	2 cut(s)	30, 2274
MaeII	ACGT	2 cut(s)	717, 2327
MaeIII	GTNAC	5 cut(s)	638, 1333, 1438, 2074, 2267
MalI	GATC	9 cut(s)	221, 369, 920, 1158, 1316, 1827, 2109, 2289, 2304
MboI	GATC	9 cut(s)	219, 367, 918, 1156, 1314, 1825, 2107, 2287, 2302
MboII	GAAGA	8 cut(s)	141, 371, 987, 1066, 1088, 1095, 2117, 2224
MfeI	CAATTG	1 cut(s)	604
MflI	RGATCY	3 cut(s)	1156, 2107, 2287
MhlI	GDGCHC	7 cut(s)	195, 675, 829, 1190, 1326, 1387, 2185
MlsI	TGGCCA	2 cut(s)	287, 1678
MluNI	TGGCCA	2 cut(s)	287, 1678
Mly113I	GGCGCC	1 cut(s)	303
MlyI	GAGTC	3 cut(s)	185, 287, 1651
MmeI	TCCRAC	5 cut(s)	456, 513, 1145, 1292, 1573
Mox20I	TGGCCA	2 cut(s)	287, 1678
MroI	TCCGGA	1 cut(s)	2112
MroNI	GCCGGC	1 cut(s)	82
MscI	TGGCCA	2 cut(s)	287, 1678
MseI	TTAA	8 cut(s)	692, 794, 936, 1028, 1254, 1557, 1854, 2049
MslI	CAYNNNNRTG	4 cut(s)	794, 1329, 1466, 1573
Msp20I	TGGCCA	2 cut(s)	287, 1678
MspA1I	CMGCKG	2 cut(s)	207, 1301
MspI	CCGG	5 cut(s)	83, 306, 465, 2113, 2165
MspR9I	CCNGG	7 cut(s)	401, 1404, 1668, 1675, 1689, 1800, 2187
MunI	CAATTG	1 cut(s)	604
Mva1269I	GAATGC	1 cut(s)	1805
MvaI	CCWGG	7 cut(s)	401, 1404, 1668, 1675, 1689, 1800, 2187
MvnI	CGCG	1 cut(s)	1516
MwoI	GCNNNNNNNGC	6 cut(s)	118, 606, 1513, 1684, 2021, 2159
NaeI	GCCGGC	1 cut(s)	84
NarI	GGCGCC	1 cut(s)	303
NdeII	GATC	9 cut(s)	219, 367, 918, 1156, 1314, 1825, 2107, 2287, 2302
NgoMIV	GCCGGC	1 cut(s)	82
NlaIII	CATG	9 cut(s)	53, 275, 410, 793, 1483, 1572, 1797, 1832, 2045
NlaIV	GGNNCC	8 cut(s)	304, 459, 1158, 1386, 1532, 1672, 1972, 2184
NmuCI	GTSAC	4 cut(s)	638, 1333, 1438, 2267
NruI	TCGCGA	1 cut(s)	1516
NspI	RCATGY	2 cut(s)	53, 275
OliI	CACNNNNGTG	1 cut(s)	1466
PagI	TCATGA	1 cut(s)	1828
PaqCI	CACCTGC	1 cut(s)	273
PciI	ACATGT	1 cut(s)	49
PcsI	WCGNNNNNNNCGW	1 cut(s)	537
PctI	GAATGC	1 cut(s)	1805
PdiI	GCCGGC	1 cut(s)	84
PfeI	GAWTC	8 cut(s)	124, 535, 875, 992, 1043, 1598, 1985, 2172
PflMI	CCANNNNNTGG	1 cut(s)	1667
PkrI	GCNGC	6 cut(s)	206, 270, 982, 1895, 2023, 2161
PleI	GAGTC	3 cut(s)	184, 286, 1651
PluTI	GGCGCC	1 cut(s)	306
PpsI	GAGTC	3 cut(s)	184, 286, 1651
Ppu21I	YACGTR	1 cut(s)	2328
PpuMI	RGGWCCY	2 cut(s)	457, 1531
PscI	ACATGT	1 cut(s)	49
PshBI	ATTAAT	1 cut(s)	1557
Psp1406I	AACGTT	1 cut(s)	717
Psp5II	RGGWCCY	2 cut(s)	457, 1531
Psp6I	CCWGG	7 cut(s)	399, 1402, 1666, 1673, 1687, 1798, 2185
PspEI	GGTNACC	1 cut(s)	638
PspFI	CCCAGC	1 cut(s)	380
PspGI	CCWGG	7 cut(s)	399, 1402, 1666, 1673, 1687, 1798, 2185
PspN4I	GGNNCC	8 cut(s)	304, 459, 1158, 1386, 1532, 1672, 1972, 2184
PspPI	GGNCC	5 cut(s)	238, 447, 457, 1343, 1531
PspPPI	RGGWCCY	2 cut(s)	457, 1531
PstNI	CAGNNNCTG	1 cut(s)	2021
PsuI	RGATCY	3 cut(s)	1156, 2107, 2287
PvuII	CAGCTG	1 cut(s)	1301
RruI	TCGCGA	1 cut(s)	1516
RsaI	GTAC	7 cut(s)	76, 656, 1066, 1238, 1297, 1972, 2094
RsaNI	GTAC	7 cut(s)	75, 655, 1065, 1237, 1296, 1971, 2093
RseI	CAYNNNNRTG	4 cut(s)	794, 1329, 1466, 1573
SaqAI	TTAA	8 cut(s)	692, 794, 936, 1028, 1254, 1557, 1854, 2049
SatI	GCNGC	6 cut(s)	205, 269, 981, 1894, 2022, 2160
Sau3AI	GATC	9 cut(s)	219, 367, 918, 1156, 1314, 1825, 2107, 2287, 2302
Sau96I	GGNCC	5 cut(s)	238, 447, 457, 1343, 1531
ScaI	AGTACT	1 cut(s)	1066
SchI	GAGTC	3 cut(s)	185, 287, 1651
ScrFI	CCNGG	7 cut(s)	401, 1404, 1668, 1675, 1689, 1800, 2187
SduI	GDGCHC	7 cut(s)	195, 675, 829, 1190, 1326, 1387, 2185
SexAI	ACCWGGT	1 cut(s)	1666
SfaNI	GCATC	6 cut(s)	170, 757, 967, 1138, 1880, 2011
SfoI	GGCGCC	1 cut(s)	304
SinI	GGWCC	4 cut(s)	447, 457, 1343, 1531
SmiI	ATTTAAAT	1 cut(s)	1255
SmiMI	CAYNNNNRTG	4 cut(s)	794, 1329, 1466, 1573
SsiI	CCGC	9 cut(s)	86, 207, 268, 397, 462, 582, 1471, 1881, 1974
SspDI	GGCGCC	1 cut(s)	302
SspMI	CTAG	2 cut(s)	30, 2274
StyD4I	CCNGG	7 cut(s)	399, 1402, 1666, 1673, 1687, 1798, 2185
StyI	CCWWGG	2 cut(s)	1200, 1534
SwaI	ATTTAAAT	1 cut(s)	1255
TaaI	ACNGT	5 cut(s)	197, 1307, 1366, 2102, 2253
TaiI	ACGT	2 cut(s)	720, 2330
TaqI	TCGA	8 cut(s)	127, 167, 281, 531, 540, 873, 990, 2301
TaqII	GACCGA	1 cut(s)	657
TatI	WGTACW	4 cut(s)	654, 1064, 1236, 1295
TauI	GCSGC	1 cut(s)	271
TfiI	GAWTC	8 cut(s)	124, 535, 875, 992, 1043, 1598, 1985, 2172
Tru1I	TTAA	8 cut(s)	692, 794, 936, 1028, 1254, 1557, 1854, 2049
Tru9I	TTAA	8 cut(s)	692, 794, 936, 1028, 1254, 1557, 1854, 2049
TseFI	GTSAC	4 cut(s)	638, 1333, 1438, 2267
TseI	GCWGC	5 cut(s)	204, 980, 1893, 2021, 2159
Tsp45I	GTSAC	4 cut(s)	638, 1333, 1438, 2267
TspDTI	ATGAA	5 cut(s)	172, 1056, 1392, 1845, 1854
TspGWI	ACGGA	1 cut(s)	2103
Van91I	CCANNNNNTGG	1 cut(s)	1667
VpaK11BI	GGWCC	4 cut(s)	447, 457, 1343, 1531
VspI	ATTAAT	1 cut(s)	1557
XapI	RAATTY	2 cut(s)	389, 2226
XceI	RCATGY	2 cut(s)	53, 275
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2183
XmiI	GTMKAC	1 cut(s)	525
XspI	CTAG	2 cut(s)	30, 2274
ZrmI	AGTACT	1 cut(s)	1066

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.