Rorug02G0598800

acetate--CoA ligase ACS, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002023
Physical Location & Seq
Reverse (-)
71921618 .. 71922395
778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0598800.1

Sequence Viewer

Length: 762 bp
ATGGATGACAATGAAGAGGTAACTGATGAATTCCAAGGCATTAAGCTCTGGTGGGCTTCCAGGAAAAGCGCACCTAGCCAATCTTCATCATTTCCTTTCTATCCTCAGTCAGATGAGAAGAGACGTTATAAGCTCACATTCCATAGACGCCACAGGGACATAATCACAGGGTCTTATCTTGACCATGTGAGAAAAGAAGGGAAGGCAATAGCAGTGAGTCATAGGCAAACGAAGCTTTACATTAACAATCCTGCTTCTGGATACAAAACGAGGAAATGGAGCTCTGTAGCCTTTGATCACCCTGCAACATTTGAGAGCATAGCAATGGAGCCAAAAAAGAAAGAGGAAATCATCAATGACCTCATGCAGTTCAGCAACGGGAAGGAGTACTACGCTAAAATTGGGAAACCTTGGAAGCGGGGTTATCTTCTGTATGGGCCACCAGGCACCGGTAAGTCCACCATGATTGCTGCCATGTCTAACCTCATGAAAAATGATCTTGAGCTGACGACAGTGAAGAGCAACACTGATCTGAGGAAGTTGCTGATTGACACGCCAAGTAAGGCTATTATTGTGATTGAGGACATTGATTGCTCACTTGATCTTACGGGCCAACGAAAGAAGAAGAAGAAGGAGAAAGATGAAGAAGATAAAGGAAAAAAGGATCCAACTTGTAAAATGAGGGAAGGAGAAGAAAGCAAAGCAAGCCAGGTTACTTTGTCTGGGCTATTAAACTTCATTTTGGTCAGCTTGCGGAGGTGA

Protein Analysis

253

Amino Acids

29.04

Weight (kDa)

9.48

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 142 - 210 1.5e-06 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 129
AccB1I GGYRCC 1 cut(s) 446
AciI CCGC 2 cut(s) 418, 754
AclWI GGATC 2 cut(s) 659, 672
AcsI RAATTY 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 148
AfaI GTAC 1 cut(s) 389
AfiI CCNNNNNNNGG 2 cut(s) 257, 449
AgeI ACCGGT 1 cut(s) 449
AjnI CCWGG 3 cut(s) 59, 442, 708
AluBI AGCT 6 cut(s) 46, 133, 235, 282, 505, 750
AluI AGCT 6 cut(s) 46, 133, 235, 282, 505, 750
Alw21I GWGCWC 1 cut(s) 284
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 2 cut(s) 659, 672
AoxI GGCC 2 cut(s) 437, 610
ApeKI GCWGC 1 cut(s) 470
ApoI RAATTY 1 cut(s) 29
AsiGI ACCGGT 1 cut(s) 449
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 2 cut(s) 437, 610
AsuHPI GGTGA 1 cut(s) 290
BamHI GGATCC 1 cut(s) 664
BanI GGYRCC 1 cut(s) 446
BanII GRGCYC 1 cut(s) 284
BarI GAAGNNNNNNTAC 2 cut(s) 374, 406
Bbv12I GWGCWC 1 cut(s) 284
BbvI GCAGC 1 cut(s) 457
BciT130I CCWGG 3 cut(s) 61, 444, 710
BciVI GTATCC 1 cut(s) 254
BclI TGATCA 1 cut(s) 295
BcoDI GTCTC 1 cut(s) 115
BfaI CTAG 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 285
BfuI GTATCC 1 cut(s) 254
BisI GCNGC 1 cut(s) 471
BlsI GCNGC 1 cut(s) 472
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 3 cut(s) 61, 444, 710
BmgT120I GGNCC 2 cut(s) 437, 610
BmiI GGNNCC 3 cut(s) 330, 448, 666
BmrFI CCNGG 3 cut(s) 61, 444, 710
BpuEI CTTGAG 1 cut(s) 521
BsaHI GRCGYC 1 cut(s) 148
BsaJI CCNNGG 2 cut(s) 34, 410
BsaWI WCCGGW 1 cut(s) 449
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 449
Bse118I RCCGGY 1 cut(s) 449
Bse3DI GCAATG 1 cut(s) 330
BseBI CCWGG 3 cut(s) 61, 444, 710
BseDI CCNNGG 2 cut(s) 34, 410
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 257, 449
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 2 cut(s) 119, 524
BseXI GCAGC 1 cut(s) 457
BshFI GGCC 2 cut(s) 439, 612
BshNI GGYRCC 1 cut(s) 446
BshTI ACCGGT 1 cut(s) 449
BsiHKAI GWGCWC 1 cut(s) 284
BsiSI CCGG 1 cut(s) 450
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 2 cut(s) 257, 449
BsmAI GTCTC 1 cut(s) 115
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 2 cut(s) 439, 612
Bsp1286I GDGCHC 1 cut(s) 284
Bsp143I GATC 5 cut(s) 295, 496, 529, 601, 664
BspACI CCGC 2 cut(s) 418, 754
BspANI GGCC 2 cut(s) 439, 612
BspCNI CTCAG 2 cut(s) 118, 525
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 3 cut(s) 330, 448, 666
BspPI GGATC 2 cut(s) 659, 672
BspQI GCTCTTC 1 cut(s) 512
BspT107I GGYRCC 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 449
BssAI RCCGGY 1 cut(s) 449
BssECI CCNNGG 2 cut(s) 34, 410
BssMI GATC 5 cut(s) 295, 496, 529, 601, 664
BssNI GRCGYC 1 cut(s) 148
BssT1I CCWWGG 2 cut(s) 34, 410
Bst2UI CCWGG 3 cut(s) 61, 444, 710
Bst4CI ACNGT 1 cut(s) 514
Bst6I CTCTTC 3 cut(s) 9, 113, 512
BstACI GRCGYC 1 cut(s) 148
BstC8I GCNNGC 2 cut(s) 706, 752
BstDEI CTNAG 2 cut(s) 105, 533
BstF5I GGATG 1 cut(s) 10
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 5 cut(s) 298, 499, 532, 604, 667
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 5 cut(s) 295, 496, 529, 601, 664
BstMWI GCNNNNNNNGC 3 cut(s) 75, 232, 705
BstNI CCWGG 3 cut(s) 61, 444, 710
BstSCI CCNGG 3 cut(s) 59, 442, 708
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 457
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BsuI GTATCC 1 cut(s) 254
BsuRI GGCC 2 cut(s) 439, 612
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 3 cut(s) 219, 519, 525
Cac8I GCNNGC 2 cut(s) 706, 752
CciI TCATGA 1 cut(s) 486
CfoI GCGC 1 cut(s) 71
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 2 cut(s) 437, 610
CseI GACGC 1 cut(s) 156
Csp6I GTAC 1 cut(s) 388
CspAI ACCGGT 1 cut(s) 449
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviAII CATG 5 cut(s) 185, 364, 463, 475, 487
CviQI GTAC 1 cut(s) 388
DdeI CTNAG 2 cut(s) 105, 533
DpnI GATC 5 cut(s) 297, 498, 531, 603, 666
DpnII GATC 5 cut(s) 295, 496, 529, 601, 664
Eam1104I CTCTTC 3 cut(s) 9, 113, 512
EarI CTCTTC 3 cut(s) 9, 113, 512
Ecl136II GAGCTC 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 34, 410
Eco24I GRGCYC 1 cut(s) 284
Eco53kI GAGCTC 1 cut(s) 282
EcoICRI GAGCTC 1 cut(s) 282
EcoRI GAATTC 1 cut(s) 29
EcoRII CCWGG 3 cut(s) 59, 442, 708
EcoT14I CCWWGG 2 cut(s) 34, 410
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 2 cut(s) 34, 410
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 5 cut(s) 188, 367, 466, 478, 490
FaqI GGGAC 1 cut(s) 170
FatI CATG 5 cut(s) 184, 363, 462, 474, 486
FauI CCCGC 1 cut(s) 411
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 1 cut(s) 471
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 1 cut(s) 471
FspBI CTAG 1 cut(s) 75
GlaI GCGC 1 cut(s) 70
GluI GCNGC 1 cut(s) 471
HaeIII GGCC 2 cut(s) 439, 612
HapII CCGG 1 cut(s) 450
HgaI GACGC 1 cut(s) 156
HhaI GCGC 1 cut(s) 71
Hin1I GRCGYC 1 cut(s) 148
Hin1II CATG 5 cut(s) 188, 367, 466, 478, 490
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 1 cut(s) 217
HpaII CCGG 1 cut(s) 450
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 1 cut(s) 459
Hpy188I TCNGA 2 cut(s) 112, 534
Hpy188III TCNNGA 4 cut(s) 179, 258, 487, 500
Hpy8I GTNNAC 1 cut(s) 459
HpyAV CCTTC 5 cut(s) 191, 196, 376, 625, 680
HpyCH4III ACNGT 1 cut(s) 514
HpyCH4IV ACGT 1 cut(s) 124
HpyCH4V TGCA 2 cut(s) 305, 367
HpyF10VI GCNNNNNNNGC 3 cut(s) 75, 232, 705
HpyF3I CTNAG 2 cut(s) 105, 533
HpySE526I ACGT 1 cut(s) 124
Hsp92I GRCGYC 1 cut(s) 148
Hsp92II CATG 5 cut(s) 188, 367, 466, 478, 490
HspAI GCGC 1 cut(s) 69
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 5 cut(s) 295, 496, 529, 601, 664
LguI GCTCTTC 1 cut(s) 512
LmnI GCTCC 2 cut(s) 279, 328
Lsp1109I GCAGC 1 cut(s) 457
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 2 cut(s) 19, 712
MalI GATC 5 cut(s) 297, 498, 531, 603, 666
MboI GATC 5 cut(s) 295, 496, 529, 601, 664
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 1 cut(s) 284
MluCI AATT 2 cut(s) 29, 399
MlyI GAGTC 1 cut(s) 226
MmeI TCCRAC 1 cut(s) 692
MseI TTAA 3 cut(s) 42, 243, 731
MslI CAYNNNNRTG 1 cut(s) 323
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 3 cut(s) 61, 444, 710
MvaI CCWGG 3 cut(s) 61, 444, 710
MwoI GCNNNNNNNGC 3 cut(s) 75, 232, 705
NdeII GATC 5 cut(s) 295, 496, 529, 601, 664
NlaIII CATG 5 cut(s) 188, 367, 466, 478, 490
NlaIV GGNNCC 3 cut(s) 330, 448, 666
PagI TCATGA 1 cut(s) 486
PciSI GCTCTTC 1 cut(s) 512
PfoI TCCNGGA 1 cut(s) 59
PinAI ACCGGT 1 cut(s) 449
PkrI GCNGC 1 cut(s) 472
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
PsiI TTATAA 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 284
Psp6I CCWGG 3 cut(s) 59, 442, 708
PspGI CCWGG 3 cut(s) 59, 442, 708
PspN4I GGNNCC 3 cut(s) 330, 448, 666
PspPI GGNCC 2 cut(s) 437, 610
PsuI RGATCY 1 cut(s) 664
RsaI GTAC 1 cut(s) 389
RsaNI GTAC 1 cut(s) 388
RseI CAYNNNNRTG 1 cut(s) 323
SacI GAGCTC 1 cut(s) 284
SapI GCTCTTC 1 cut(s) 512
SaqAI TTAA 3 cut(s) 42, 243, 731
SatI GCNGC 1 cut(s) 471
Sau3AI GATC 5 cut(s) 295, 496, 529, 601, 664
Sau96I GGNCC 2 cut(s) 437, 610
ScaI AGTACT 1 cut(s) 389
SchI GAGTC 1 cut(s) 226
ScrFI CCNGG 3 cut(s) 61, 444, 710
SduI GDGCHC 1 cut(s) 284
SfcI CTRYAG 1 cut(s) 285
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
Sse9I AATT 2 cut(s) 29, 399
SsiI CCGC 2 cut(s) 418, 754
SspMI CTAG 1 cut(s) 75
SstI GAGCTC 1 cut(s) 284
StyD4I CCNGG 3 cut(s) 59, 442, 708
StyI CCWWGG 2 cut(s) 34, 410
TaaI ACNGT 1 cut(s) 514
TaiI ACGT 1 cut(s) 127
TasI AATT 2 cut(s) 29, 399
TatI WGTACW 1 cut(s) 387
Tru1I TTAA 3 cut(s) 42, 243, 731
Tru9I TTAA 3 cut(s) 42, 243, 731
TscAI CASTG 3 cut(s) 219, 519, 532
TseI GCWGC 1 cut(s) 470
TspDTI ATGAA 6 cut(s) 27, 42, 75, 503, 657, 727
TspRI CASTG 3 cut(s) 219, 519, 532
XapI RAATTY 1 cut(s) 29
XspI CTAG 1 cut(s) 75
ZrmI AGTACT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.