Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002023

Physical Location & Seq

Genomic Coordinates Reverse (-)

288531 .. 297445

Gene Structure 8915 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1003300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGGAATCATCAACGCCTGATCATGCCGATCATCATGCTGCTAACAACAACAACAAGAAGCCTCGTAATAATCCAAATTTGATTACGACAAGCGATCACCTGCGGCATGTGGAGTCGATGGCCACAATGCCTTCCGGCGCCGGAAACATTTCCCACCCGAACGCTGTCATTCTCGGAGAATCTCTCGCTTCTGAAGAGAACGATCTCGTCTTTCCCAGCGACCACTTCTCCCGTCAGGCCCACGTCTCCTCTCCCCAACAGTATCTGGAGATGTACAGACGGTCCATCGAGGACCCCGCCGGATTTTGGTCCGACATCGCTGCCACCTTCTTTTGGAAACAGAAATGGGGCCAACCAGTGTACTCGGAGAATCTTGATGTCAGGAAAGGCGATATCCGAATTGAGTGGTTCAGGGGCGGTATCACCAACATCTGCTACAATTGCCTTGACACAAATGTTGAAGCTGGACTTGGTGACAAAATTGCCTTCTACTGGGAAGGCAATGAGCTTGGTGTTGATGCCACTTTAACTTACACCCAGCTTCTCCACAACGTTTGCCAGCTTGCGAACTATTTGAAAGATGTTGGAGTTAAAAAGGGTGATGCTGTTGTGGTTTACCTGCCCATGTTAATGGAACTTCCAATTACAATGCTTGCTTGTGCCCGCATTGGTGCCGTTCACTCGGTTGTCTTTGCCGGATTTTCTGCGGAATCTCTTGCTCAAAGGATTGTAGATTGCAAACCGAAAGTTGTAATCACTTGCAATGCTGTTAAAAGAGGTTCTAAGGTTATCCACCTCAAAGATATAGTTGATGCTGCCCTTATAGAATCATCCCAAAGTGGGGTTTCTGTAGATGTATGCTTAACTTATGAAAATCAATTGGCTATGAAGAGGGAAAGTACTAAATGGCTGGAAGGAAGGGATGTATGGTGGCAGGATGTCATACCTAAATATCCAACTACTTGTGCAGTGGACTGGGTTGATGCAGAAGATCCACTTTTTCTGCTCTATACCAGTGGGAGCACTGGCAAACCGAAGGGAGTTCTCCACACAACTGGAGGATATATGGTGTACGCTGCCACAACATTTAAATATGCATTTGATTACAGCTCATCCGACATCTACTGGTGTACTGCTGACTGTGGTTGGATCACTGGGCACAGCTATGTCACTTATGGACCCCTGCTTAATGGAGCAACTGCTGTTTTATATGAAGGGGCTCCAAATTATCCTGATCCTGGACGCTGTTGGGATGTTGTTGATAAATACAAAGTGACCATATTTTATACTGCCCCCACATTAGTGCGGTCCCTCATGCGTGATAGTGACAAGTATGTTACACGATACTCGCGAAAATCCTTACGGGTCCTTGGAAGTGTAGGCGAGCCTATTAACCCAAGTGCATGGAGGTGGTTTTTCAACGTGGTTGGAGATTCAAAATGTCCTATTTCCGACACTTGGTGGCAAACTGAAACTGGTGGCTTTATGATAACTCCACTACCGGGTGCCTGGCCACAGAAACCTGGTTCTGCTACTTTCCCTTTCTTTGGGGTGCAGGCTGTCATAGTTGATGAAAAGGGTGTTGAGATTGAAGGGGAATGCAGTGGCTATCTGTGTGTAAAAAGCTCATGGCCTGGGGCTTTCCGAACTCTATACGGTGATCACGAAAGATACGAAACCACTTACTTTAAACCTTTCCCCGGATATTATTTTAGTGGTGATGGATGCAGCAGGGACAAGGATGGATATCACTGGCTTACAGGAAGGGTTGATGATGTTATCAACGTTAGTGGACATCGTATTGGTACAGCAGAAGTGGAATCTGCTCTGGTCTCCCATCCCCAGTGTGCTGAAGCTGCTGTGGTTGGCATTGAGCATGAAGTTAAAGGACAAGGTATATATGCCTTTGTTACTCTTGTGGAAGGTGTACCTTACAGTGAAGAACTCCGGAAAAGCCTTGTACTGGCTGTGAGAAAGCAGATAGGAGCATTTGCAGCACCGGACAGAATCCACTGGGCCCCTGGCCTTCCGAAAACAAGGAGTGGGAAGATCATGAGGAGGATTCTGAGGAAGATCGCTTCTAGACAGTTAGATGAGCTTGGAGACACCAGCACCCTTGCGGAACCAAATGTGGTCGATCAACTTATTGCTCTCGCAGATGACATGCGACTTCCTCTTGAGAAAAAAGTGATCAAACAGATGAATTTTGGAAGGATTCCAATTGGAGAATGTTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003987 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006082 GO:0006083 GO:0008150 GO:0008152 GO:0009507 GO:0009514 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017144 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00010 ko00620 ko00640 ko00680 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00620 map00640 map00680 map00720 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

ACAS_N AMP-binding AMP-binding_C

Protein Analysis

746

Amino Acids

82.82

Weight (kDa)

5.92

Isoelectric Point (pI)

32.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACAS_N	PF16177	88 - 148	2.3e-17	Acetyl-coenzyme A synthetase N-terminus
AMP-binding	PF00501	157 - 543	4.7e-91	AMP-binding enzyme
AMP-binding_C	PF13193	605 - 683	4.7e-24	AMP-binding enzyme C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G36880 AT5G36880 AT5G36880 AT5G36880 AT5G36880 AT5G36880
fragaria_vesca	FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480
malus_domestica	MD09G1000400.v1.1 MD17G1003300.v1.1
prunus_persica	Prupe.3G315800_v2.0.a1
pyrus_communis	pycom111g00030 pycom17g00010
rosa_chinensis	RchiOBHm_Chr2g0176771
rosa_multiflora	Rmu_sc0020992.1_g000001
rosa_roxburghii	Rroxscaffold_2G00076170
rosa_rugosa	Rorug02G0598400 Rorug02G0598500 Rorug02G0598600 Rorug02G0598700 Rorug02G0598800 Rorug02G0598900 Rorug02G0599000 Rorug02G0599100 Rorug02G0599200
rosa_samantha	Rh2AG676000 Rh2BG687100 Rh2CG650500
rosa_wichuraiana	Rw2G055470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	108
Acc36I	ACCTGC	2 cut(s)	108, 627
AccB1I	GGYRCC	3 cut(s)	137, 671, 1505
AccII	CGCG	1 cut(s)	1351
AccIII	TCCGGA	1 cut(s)	1947
AciI	CCGC	7 cut(s)	103, 297, 417, 664, 707, 1306, 2120
AclI	AACGTT	2 cut(s)	552, 1785
AclWI	GGATC	3 cut(s)	986, 1157, 1229
AcoI	YGGCCR	2 cut(s)	120, 1511
AcsI	RAATTY	2 cut(s)	76, 2203
AcuI	CTGAAG	2 cut(s)	213, 1872
AcyI	GRCGYC	1 cut(s)	138
AdeI	CACNNNGTG	1 cut(s)	1461
AfaI	GTAC	8 cut(s)	275, 362, 901, 1073, 1132, 1807, 1929, 1962
AgsI	TTSAA	5 cut(s)	461, 577, 1420, 1437, 1592
AjiI	CACGTC	1 cut(s)	244
AjnI	CCWGG	5 cut(s)	1237, 1508, 1522, 1633, 2020
AjuI	GAANNNNNNNTTGG	2 cut(s)	453, 485
AleI	CACNNNNGTG	1 cut(s)	1301
AluBI	AGCT	9 cut(s)	464, 508, 541, 562, 1110, 1164, 1626, 1856, 2098
AluI	AGCT	9 cut(s)	464, 508, 541, 562, 1110, 1164, 1626, 1856, 2098
Alw21I	GWGCWC	1 cut(s)	1025
Alw26I	GTCTC	3 cut(s)	250, 1837, 2097
AlwI	GGATC	3 cut(s)	986, 1157, 1229
AlwNI	CAGNNNCTG	1 cut(s)	265
Aor13HI	TCCGGA	1 cut(s)	1947
AoxI	GGCC	7 cut(s)	120, 237, 349, 1511, 1631, 2016, 2023
ApaI	GGGCCC	1 cut(s)	2020
ApeKI	GCWGC	7 cut(s)	38, 320, 815, 1076, 1728, 1856, 1994
ApoI	RAATTY	2 cut(s)	76, 2203
Asp700I	GAANNNNTTC	1 cut(s)	148
AspLEI	GCGC	1 cut(s)	140
AsuC2I	CCSGG	2 cut(s)	1503, 1701
AsuHPI	GGTGA	6 cut(s)	89, 415, 485, 611, 1670, 1730
AvaII	GGWCC	6 cut(s)	282, 292, 309, 1178, 1308, 1366
BaeGI	GKGCMC	3 cut(s)	664, 1161, 2020
BalI	TGGCCA	2 cut(s)	122, 1513
BanI	GGYRCC	3 cut(s)	137, 671, 1505
BanII	GRGCYC	2 cut(s)	1222, 2020
Bbv12I	GWGCWC	1 cut(s)	1025
BbvI	GCAGC	7 cut(s)	25, 307, 802, 1063, 1740, 1843, 2006
BccI	CCATC	5 cut(s)	112, 293, 1715, 1736, 1845
BceAI	ACGGC	1 cut(s)	659
BcgI	CGANNNNNNTGC	4 cut(s)	17, 51, 302, 336
BciT130I	CCWGG	5 cut(s)	1239, 1510, 1524, 1635, 2022
BclI	TGATCA	3 cut(s)	19, 1660, 2190
BcnI	CCSGG	2 cut(s)	1503, 1701
BcoDI	GTCTC	3 cut(s)	250, 1837, 2097
BfaI	CTAG	1 cut(s)	2082
BfmI	CTRYAG	1 cut(s)	849
BfoI	RGCGCY	1 cut(s)	141
BfuAI	ACCTGC	2 cut(s)	108, 627
BisI	GCNGC	8 cut(s)	39, 104, 321, 816, 1077, 1729, 1857, 1995
BlsI	GCNGC	8 cut(s)	40, 105, 322, 817, 1078, 1730, 1858, 1996
BmcAI	AGTACT	1 cut(s)	901
Bme1390I	CCNGG	7 cut(s)	1239, 1503, 1510, 1524, 1635, 1701, 2022
Bme18I	GGWCC	6 cut(s)	282, 292, 309, 1178, 1308, 1366
BmgBI	CACGTC	1 cut(s)	244
BmrFI	CCNGG	7 cut(s)	1239, 1503, 1510, 1524, 1635, 1701, 2022
BmrI	ACTGGG	5 cut(s)	502, 985, 1164, 1837, 2023
BmsI	GCATC	5 cut(s)	508, 592, 802, 973, 1715
BmuI	ACTGGG	5 cut(s)	502, 985, 1164, 1837, 2023
BplI	GAGNNNNNCTC	2 cut(s)	168, 200
BpmI	CTGGAG	2 cut(s)	287, 1077
BpuEI	CTTGAG	1 cut(s)	2198
BpuMI	CCSGG	2 cut(s)	1503, 1701
BsaBI	GATNNNNATC	1 cut(s)	1746
BsaHI	GRCGYC	1 cut(s)	138
BsaI	GGTCTC	1 cut(s)	1837
BsaJI	CCNNGG	4 cut(s)	1369, 1634, 1699, 2020
BsaWI	WCCGGW	2 cut(s)	1947, 1999
BsaXI	ACNNNNNCTCC	2 cut(s)	212, 242
Bse3DI	GCAATG	2 cut(s)	508, 769
Bse8I	GATNNNNATC	1 cut(s)	1746
BseAI	TCCGGA	1 cut(s)	1947
BseBI	CCWGG	5 cut(s)	1239, 1510, 1524, 1635, 2022
BseDI	CCNNGG	4 cut(s)	1369, 1634, 1699, 2020
BseGI	GGATG	8 cut(s)	830, 928, 943, 1112, 1258, 1730, 1747, 1837
BseJI	GATNNNNATC	1 cut(s)	1746
BseMI	GCAATG	2 cut(s)	508, 769
BseMII	CTCAG	1 cut(s)	2057
BseRI	GAGGAG	2 cut(s)	238, 2071
BseSI	GKGCMC	3 cut(s)	664, 1161, 2020
BseXI	GCAGC	7 cut(s)	25, 307, 802, 1063, 1740, 1843, 2006
BseYI	CCCAGC	2 cut(s)	215, 537
BsgI	GTGCAG	2 cut(s)	987, 1574
Bsh1236I	CGCG	1 cut(s)	1351
BshFI	GGCC	7 cut(s)	122, 239, 351, 1513, 1633, 2018, 2025
BshNI	GGYRCC	3 cut(s)	137, 671, 1505
BsiHKAI	GWGCWC	1 cut(s)	1025
BsiSI	CCGG	8 cut(s)	135, 141, 300, 696, 1502, 1701, 1948, 2000
BslFI	GGGAC	2 cut(s)	1294, 1748
BsmAI	GTCTC	3 cut(s)	250, 1837, 2097
BsmBI	CGTCTC	1 cut(s)	250
BsmFI	GGGAC	2 cut(s)	1294, 1748
BsmI	GAATGC	1 cut(s)	1604
BsnI	GGCC	7 cut(s)	122, 239, 351, 1513, 1633, 2018, 2025
Bso31I	GGTCTC	1 cut(s)	1837
Bsp120I	GGGCCC	1 cut(s)	2016
Bsp1286I	GDGCHC	5 cut(s)	664, 1025, 1161, 1222, 2020
Bsp13I	TCCGGA	1 cut(s)	1947
Bsp1407I	TGTACA	1 cut(s)	273
Bsp68I	TCGCGA	1 cut(s)	1351
BspACI	CCGC	7 cut(s)	103, 297, 417, 664, 707, 1306, 2120
BspANI	GGCC	7 cut(s)	122, 239, 351, 1513, 1633, 2018, 2025
BspCNI	CTCAG	1 cut(s)	2058
BspEI	TCCGGA	1 cut(s)	1947
BspFNI	CGCG	1 cut(s)	1351
BspHI	TCATGA	1 cut(s)	2052
BspMI	ACCTGC	2 cut(s)	108, 627
BspPI	GGATC	3 cut(s)	986, 1157, 1229
BspT107I	GGYRCC	3 cut(s)	137, 671, 1505
BspTNI	GGTCTC	1 cut(s)	1837
BsrDI	GCAATG	2 cut(s)	508, 769
BsrGI	TGTACA	1 cut(s)	273
BssECI	CCNNGG	4 cut(s)	1369, 1634, 1699, 2020
BssNI	GRCGYC	1 cut(s)	138
BssT1I	CCWWGG	1 cut(s)	1369
Bst2UI	CCWGG	5 cut(s)	1239, 1510, 1524, 1635, 2022
Bst4CI	ACNGT	6 cut(s)	261, 282, 1142, 1658, 1937, 2088
Bst6I	CTCTTC	2 cut(s)	189, 884
BstACI	GRCGYC	1 cut(s)	138
BstAUI	TGTACA	1 cut(s)	273
BstC8I	GCNNGC	6 cut(s)	560, 564, 654, 664, 1385, 1557
BstDEI	CTNAG	2 cut(s)	783, 2066
BstF5I	GGATG	8 cut(s)	830, 928, 943, 1112, 1258, 1730, 1747, 1837
BstFNI	CGCG	1 cut(s)	1351
BstH2I	RGCGCY	1 cut(s)	141
BstHHI	GCGC	1 cut(s)	140
BstMAI	GTCTC	3 cut(s)	250, 1837, 2097
BstMWI	GCNNNNNNNGC	3 cut(s)	441, 1856, 1994
BstNI	CCWGG	5 cut(s)	1239, 1510, 1524, 1635, 2022
BstNSI	RCATGY	2 cut(s)	110, 2167
BstSCI	CCNGG	7 cut(s)	1237, 1501, 1508, 1522, 1633, 1699, 2020
BstSFI	CTRYAG	1 cut(s)	849
BstSLI	GKGCMC	3 cut(s)	664, 1161, 2020
BstUI	CGCG	1 cut(s)	1351
BstV1I	GCAGC	7 cut(s)	25, 307, 802, 1063, 1740, 1843, 2006
BstX2I	RGATCY	1 cut(s)	991
BstXI	CCANNNNNNTGG	3 cut(s)	631, 1055, 1404
BstYI	RGATCY	1 cut(s)	991
BsuRI	GGCC	7 cut(s)	122, 239, 351, 1513, 1633, 2018, 2025
BtgZI	GCGATG	1 cut(s)	301
BtrI	CACGTC	1 cut(s)	244
BtsCI	GGATG	8 cut(s)	830, 928, 943, 1112, 1258, 1730, 1747, 1837
BtsI	GCAGTG	2 cut(s)	975, 1609
BtuMI	TCGCGA	1 cut(s)	1351
BveI	ACCTGC	2 cut(s)	108, 627
Cac8I	GCNNGC	6 cut(s)	560, 564, 654, 664, 1385, 1557
CaiI	CAGNNNCTG	1 cut(s)	265
CciI	TCATGA	1 cut(s)	2052
CfoI	GCGC	1 cut(s)	140
CseI	GACGC	1 cut(s)	1251
CsiI	ACCWGGT	1 cut(s)	1522
Csp6I	GTAC	8 cut(s)	274, 361, 900, 1072, 1131, 1806, 1928, 1961
CspCI	CAANNNNNGTGG	4 cut(s)	536, 571, 1771, 1806
CviQI	GTAC	8 cut(s)	274, 361, 900, 1072, 1131, 1806, 1928, 1961
DdeI	CTNAG	2 cut(s)	783, 2066
DinI	GGCGCC	1 cut(s)	139
DraI	TTTAAA	2 cut(s)	1090, 1690
DraIII	CACNNNGTG	1 cut(s)	1461
EaeI	YGGCCR	2 cut(s)	120, 1511
Eam1104I	CTCTTC	2 cut(s)	189, 884
EarI	CTCTTC	2 cut(s)	189, 884
Eco130I	CCWWGG	1 cut(s)	1369
Eco24I	GRGCYC	2 cut(s)	1222, 2020
Eco31I	GGTCTC	1 cut(s)	1837
Eco32I	GATATC	2 cut(s)	394, 1748
Eco47I	GGWCC	6 cut(s)	282, 292, 309, 1178, 1308, 1366
Eco57I	CTGAAG	2 cut(s)	213, 1872
EcoO109I	RGGNCCY	3 cut(s)	292, 1366, 2017
EcoRII	CCWGG	5 cut(s)	1237, 1508, 1522, 1633, 2020
EcoRV	GATATC	2 cut(s)	394, 1748
EcoT14I	CCWWGG	1 cut(s)	1369
EcoT22I	ATGCAT	1 cut(s)	1099
EcoT38I	GRGCYC	2 cut(s)	1222, 2020
EgeI	GGCGCC	1 cut(s)	139
EheI	GGCGCC	1 cut(s)	139
ErhI	CCWWGG	1 cut(s)	1369
Esp3I	CGTCTC	1 cut(s)	250
FalI	AAGNNNNNCTT	4 cut(s)	453, 485, 981, 1013
FaqI	GGGAC	2 cut(s)	1294, 1748
FauI	CCCGC	2 cut(s)	304, 671
FbaI	TGATCA	3 cut(s)	19, 1660, 2190
Fnu4HI	GCNGC	8 cut(s)	39, 104, 321, 816, 1077, 1729, 1857, 1995
FokI	GGATG	8 cut(s)	817, 935, 950, 1099, 1265, 1737, 1754, 1824
FriOI	GRGCYC	2 cut(s)	1222, 2020
Fsp4HI	GCNGC	8 cut(s)	39, 104, 321, 816, 1077, 1729, 1857, 1995
FspBI	CTAG	1 cut(s)	2082
GlaI	GCGC	1 cut(s)	139
GluI	GCNGC	8 cut(s)	39, 104, 321, 816, 1077, 1729, 1857, 1995
GsaI	CCCAGC	2 cut(s)	219, 541
GsuI	CTGGAG	2 cut(s)	287, 1077
HaeII	RGCGCY	1 cut(s)	141
HaeIII	GGCC	7 cut(s)	122, 239, 351, 1513, 1633, 2018, 2025
HapII	CCGG	8 cut(s)	135, 141, 300, 696, 1502, 1701, 1948, 2000
HgaI	GACGC	1 cut(s)	1251
HhaI	GCGC	1 cut(s)	140
Hin1I	GRCGYC	1 cut(s)	138
Hin6I	GCGC	1 cut(s)	138
HinP1I	GCGC	1 cut(s)	138
HpaII	CCGG	8 cut(s)	135, 141, 300, 696, 1502, 1701, 1948, 2000
HphI	GGTGA	6 cut(s)	89, 415, 485, 611, 1670, 1730
Hpy166II	GTNNAC	8 cut(s)	361, 616, 679, 973, 1072, 1131, 1793, 1928
Hpy8I	GTNNAC	8 cut(s)	361, 616, 679, 973, 1072, 1131, 1793, 1928
HpyCH4III	ACNGT	6 cut(s)	261, 282, 1142, 1658, 1937, 2088
HpyCH4IV	ACGT	4 cut(s)	243, 552, 1422, 1785
HpyF10VI	GCNNNNNNNGC	3 cut(s)	441, 1856, 1994
HpyF3I	CTNAG	2 cut(s)	783, 2066
HpySE526I	ACGT	4 cut(s)	243, 552, 1422, 1785
Hsp92I	GRCGYC	1 cut(s)	138
HspAI	GCGC	1 cut(s)	138
KasI	GGCGCC	1 cut(s)	137
Kpn2I	TCCGGA	1 cut(s)	1947
Ksp22I	TGATCA	3 cut(s)	19, 1660, 2190
LmnI	GCTCC	4 cut(s)	1020, 1193, 1225, 1985
Lsp1109I	GCAGC	7 cut(s)	25, 307, 802, 1063, 1740, 1843, 2006
LweI	GCATC	5 cut(s)	508, 592, 802, 973, 1715
MabI	ACCWGGT	1 cut(s)	1522
MaeI	CTAG	1 cut(s)	2082
MaeII	ACGT	4 cut(s)	243, 552, 1422, 1785
MaeIII	GTNAC	6 cut(s)	473, 1168, 1273, 1325, 1336, 1909
MboII	GAAGA	6 cut(s)	206, 901, 1001, 1952, 2059, 2083
MfeI	CAATTG	3 cut(s)	439, 878, 2220
MflI	RGATCY	1 cut(s)	991
MhlI	GDGCHC	5 cut(s)	664, 1025, 1161, 1222, 2020
MlsI	TGGCCA	2 cut(s)	122, 1513
MluCI	AATT	9 cut(s)	76, 399, 439, 480, 642, 878, 1225, 2203, 2220
MluNI	TGGCCA	2 cut(s)	122, 1513
Mly113I	GGCGCC	1 cut(s)	138
MlyI	GAGTC	1 cut(s)	122
MmeI	TCCRAC	7 cut(s)	336, 565, 980, 1127, 1140, 1408, 1476
Mox20I	TGGCCA	2 cut(s)	122, 1513
Mph1103I	ATGCAT	1 cut(s)	1099
MroI	TCCGGA	1 cut(s)	1947
MroXI	GAANNNNTTC	1 cut(s)	148
MscI	TGGCCA	2 cut(s)	122, 1513
MslI	CAYNNNNRTG	4 cut(s)	629, 1164, 1301, 1408
Msp20I	TGGCCA	2 cut(s)	122, 1513
MspI	CCGG	8 cut(s)	135, 141, 300, 696, 1502, 1701, 1948, 2000
MspR9I	CCNGG	7 cut(s)	1239, 1503, 1510, 1524, 1635, 1701, 2022
MunI	CAATTG	3 cut(s)	439, 878, 2220
Mva1269I	GAATGC	1 cut(s)	1604
MvaI	CCWGG	5 cut(s)	1239, 1510, 1524, 1635, 2022
MvnI	CGCG	1 cut(s)	1351
MwoI	GCNNNNNNNGC	3 cut(s)	441, 1856, 1994
NarI	GGCGCC	1 cut(s)	138
NciI	CCSGG	2 cut(s)	1503, 1701
NmuCI	GTSAC	4 cut(s)	473, 1168, 1273, 1325
NruI	TCGCGA	1 cut(s)	1351
NsiI	ATGCAT	1 cut(s)	1099
NspI	RCATGY	2 cut(s)	110, 2167
OliI	CACNNNNGTG	1 cut(s)	1301
PagI	TCATGA	1 cut(s)	2052
PaqCI	CACCTGC	1 cut(s)	108
PcsI	WCGNNNNNNNCGW	3 cut(s)	1348, 1662, 1671
PctI	GAATGC	1 cut(s)	1604
PdmI	GAANNNNTTC	1 cut(s)	148
PfoI	TCCNGGA	1 cut(s)	1237
PkrI	GCNGC	8 cut(s)	40, 105, 322, 817, 1078, 1730, 1858, 1996
PleI	GAGTC	1 cut(s)	121
PluTI	GGCGCC	1 cut(s)	141
PpsI	GAGTC	1 cut(s)	121
PpuMI	RGGWCCY	2 cut(s)	292, 1366
Psp1406I	AACGTT	2 cut(s)	552, 1785
Psp5II	RGGWCCY	2 cut(s)	292, 1366
Psp6I	CCWGG	5 cut(s)	1237, 1508, 1522, 1633, 2020
PspFI	CCCAGC	2 cut(s)	215, 537
PspGI	CCWGG	5 cut(s)	1237, 1508, 1522, 1633, 2020
PspOMI	GGGCCC	1 cut(s)	2016
PspPPI	RGGWCCY	2 cut(s)	292, 1366
PstNI	CAGNNNCTG	1 cut(s)	265
PsuI	RGATCY	1 cut(s)	991
RruI	TCGCGA	1 cut(s)	1351
RsaI	GTAC	8 cut(s)	275, 362, 901, 1073, 1132, 1807, 1929, 1962
RsaNI	GTAC	8 cut(s)	274, 361, 900, 1072, 1131, 1806, 1928, 1961
RseI	CAYNNNNRTG	4 cut(s)	629, 1164, 1301, 1408
SatI	GCNGC	8 cut(s)	39, 104, 321, 816, 1077, 1729, 1857, 1995
ScaI	AGTACT	1 cut(s)	901
SchI	GAGTC	1 cut(s)	122
ScrFI	CCNGG	7 cut(s)	1239, 1503, 1510, 1524, 1635, 1701, 2022
SduI	GDGCHC	5 cut(s)	664, 1025, 1161, 1222, 2020
SexAI	ACCWGGT	1 cut(s)	1522
SfaNI	GCATC	5 cut(s)	508, 592, 802, 973, 1715
SfcI	CTRYAG	1 cut(s)	849
SfoI	GGCGCC	1 cut(s)	139
SinI	GGWCC	6 cut(s)	282, 292, 309, 1178, 1308, 1366
SmiI	ATTTAAAT	1 cut(s)	1090
SmiMI	CAYNNNNRTG	4 cut(s)	629, 1164, 1301, 1408
SmlI	CTYRAG	1 cut(s)	2177
SmoI	CTYRAG	1 cut(s)	2177
Sse9I	AATT	9 cut(s)	76, 399, 439, 480, 642, 878, 1225, 2203, 2220
SsiI	CCGC	7 cut(s)	103, 297, 417, 664, 707, 1306, 2120
SspDI	GGCGCC	1 cut(s)	137
SspMI	CTAG	1 cut(s)	2082
StyD4I	CCNGG	7 cut(s)	1237, 1501, 1508, 1522, 1633, 1699, 2020
StyI	CCWWGG	1 cut(s)	1369
SwaI	ATTTAAAT	1 cut(s)	1090
TaaI	ACNGT	6 cut(s)	261, 282, 1142, 1658, 1937, 2088
TaiI	ACGT	4 cut(s)	246, 555, 1425, 1788
TaqI	TCGA	3 cut(s)	116, 288, 2136
TasI	AATT	9 cut(s)	76, 399, 439, 480, 642, 878, 1225, 2203, 2220
TatI	WGTACW	5 cut(s)	273, 360, 899, 1130, 1960
TauI	GCSGC	1 cut(s)	106
TseFI	GTSAC	4 cut(s)	473, 1168, 1273, 1325
TseI	GCWGC	7 cut(s)	38, 320, 815, 1076, 1728, 1856, 1994
Tsp45I	GTSAC	4 cut(s)	473, 1168, 1273, 1325
TspDTI	ATGAA	6 cut(s)	885, 902, 1227, 1587, 1893, 2216
VpaK11BI	GGWCC	6 cut(s)	282, 292, 309, 1178, 1308, 1366
XapI	RAATTY	2 cut(s)	76, 2203
XbaI	TCTAGA	1 cut(s)	2081
XceI	RCATGY	2 cut(s)	110, 2167
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2018
XmnI	GAANNNNTTC	1 cut(s)	148
XspI	CTAG	1 cut(s)	2082
ZrmI	AGTACT	1 cut(s)	901
Zsp2I	ATGCAT	1 cut(s)	1099

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.