Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp03

Orthogroup ID

OG0002023

Physical Location & Seq

Genomic Coordinates Forward (+)

27245723 .. 27252594

Gene Structure 6872 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.3G315800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2367 bp

ATGGTCGCTCAATATTACAATACGAATTATTATAATATAATTGTTGCTGAACCTCCTCCGGTAGCCATACTAACGTCGTATTCCGAGCTCAAGAGAAGAAAGTCAATTCCAAATTCGGTTCCTCCTACTCCTCCTCCTCTTAGTCTTACTTCTTCTTCTTCTTCTTTGTGGGGAAGATCTAGATTTAAATCCTGGGGTTGGGGCACCAGTAGCGGCAGCAGCATTATGAGCACTAGTAATAATAAGGTGATTTGGGGCATGGAATCATCATCTGCCAACAACATTACCACAACCAATCATCTGCGGCATGTGGAGTCCATGGCCACAATGCCTTCTGGCGCTGGAAACATTCCCCACCTCAACGCCATCGTTTTGGGAGAAGCACTCGCTTTTGAAGAGAACGATCTCATCTTCCCCAGCGACGACTTTTCCCGCCAGGCTCATGTTCCCTCTCCCGGCAAGTATCTGGAGATGTATAAGAGGTCCATCGAGGACCCCGCGGGGTTTTGGTCGGATATTGCTTCCAGCTTCTATTGGAAACAGAAATGGGGCCAACAAGTCTACTTGGAGAATCTCGATGTCAGGAAAGGCGATATCAGAATTGAGTGGTTCAAGGGCGGTATCACCAACATCTGCTACAATTGCCTTGACTCAAATGTTGAAGCTGGACTTGGTGACAAAATTGCCTTATACTGGGAAGGCAATGAGCCTGGTGTTGATGCCACTTTAACGTACATCCAGCTTCTCCACAACGTTTGCCAGCTGGCAAACTATTTGAAAGATATTGGAGTTAAAAAGGGTGATGCTGTTGTGGTTTACTTACCAATGTTAATGGAACTTCCAATCACAATGCTTGCTTGTGCTCGCATTGGTGCAGTTCACTCGGTTGTCTTTGCTGGATTTTCCGCAGAATCTCTTGCTCAAAGAATTGTGGATTGCAAACCGAAAGTTGTAATCACTTGCAATGCTGTTAAAAGGGGTTCTAAGGTCATCCACCTGAAAGATATAGTTGATGCTGCCCTTATTGAATCATCCCAAAGTGGGGTTTCTATAGATGTATGCTTAACTTATGAAAATCAATTGGCTATGAAGAAGGAAAGTACCAAATGGAAAGAAGAAAGAGATGTATGGTGGCAGGATGTCATACCTAAATATCCAACTACATGTGCAGTGGAGTGGGTTGATGCAGAAGATCCACTTTTTCTGCTCTATACTAGTGGGAGCACTGGGAAGCCGAAGGGAGTTGTCCACACAACTGGAGGATATATGGTGTACACTGCAACAACATTTAAATATGCATTTGATTACAAGCCATCTGACATCTACTGGTGTACAGCTGACTGTGGTTGGATCACTGGGCACAGCTATGTCACATATGGACCCCTGCTAAATGGAGCGTCTGCTGTCATATTTGAAGGGGCTCCAAATTATCCTAATCCTGGACGCTGTTGGGATATTGTTGATAAATACCAAGTGACCATATTTTATACTGCCCCCACATTAGTGCGGTCCCTCATGCGTGATAGTGACGAGTATGTTACGCGATACTCGCGGAAATCCTTACGGGTCCTTGGAAGTGTAGGCGAGCCTATTAATCCAAGTGCATGGAGATGGTTTTTCAATCTGGTTGGAGATTCAAAATGTCCTATTTCTGACACTTGGTGGCAAACTGAAACTGGTGGCTTTATGATAACTCCATTACCGGGTGCCTGGCCACAGAAGCCTGGTTCTGCTACTTTCCCTTTCTTTGGAGTTCAGGCTGTCATAGTGGATGAGAAGGGTGTTGAGATTGAAGGGGAGTGCAGTGGTTATCTGTGTGTAAAAAGCTCATGGCCTGGAGCATTCCGAACTCTTTACTGTGATCATGAAAGATACGAAACTACTTACTTTAAACCTTTCCCTGGGTATTATTTTAGTGGTGATGGATGCAGCAGGGACAAAGATGGATATCACTGGCTTACAGGACGGGTTGATGATGTTATCAATGTCAGTGGACATCGTATTGGTACAGCAGAAGTGGAATCTGCTCTGGTATCACATCCCCAGTGTGCTGAAGCTGCTGTGGTTGGCGTTGAGCATGAGGTTAAAGGACAAGGTTTATATGCCTTTGTTACTCTTGTGGAGGGTGTACCTTACAGCGAAGAACTCCGGAAAAGCCTTATACTGAATGTCAGAAAGCAGATCGGAGCATTTGCAGCACCGGACAAAGTCCACTGGGCCCCTGGCCTTCCAAAAACAAGGAGTGGGAAGATAATGAGGAGGATTCTGAGGAAAATTGCTTCTAGACAGTTAGATGAGCTTGGTGACACCAGCACTCTTGCAGATCCAAATGTGGTCGATCAACTTATTGCTCTCGCAGATGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003987 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006082 GO:0006083 GO:0008150 GO:0008152 GO:0009507 GO:0009514 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016405 GO:0016874 GO:0016877 GO:0016878 GO:0016999 GO:0017144 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00010 ko00620 ko00640 ko00680 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00620 map00640 map00680 map00720 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

ACAS_N AMP-binding AMP-binding_C

Protein Analysis

789

Amino Acids

87.5

Weight (kDa)

5.99

Isoelectric Point (pI)

35.82

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G36880 AT5G36880 AT5G36880 AT5G36880 AT5G36880 AT5G36880
fragaria_vesca	FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480 FvH4_6g54480
malus_domestica	MD09G1000400.v1.1 MD17G1003300.v1.1
prunus_persica	Prupe.3G315800_v2.0.a1
pyrus_communis	pycom111g00030 pycom17g00010
rosa_chinensis	RchiOBHm_Chr2g0176771
rosa_multiflora	Rmu_sc0020992.1_g000001
rosa_roxburghii	Rroxscaffold_2G00076170
rosa_rugosa	Rorug02G0598400 Rorug02G0598500 Rorug02G0598600 Rorug02G0598700 Rorug02G0598800 Rorug02G0598900 Rorug02G0599000 Rorug02G0599100 Rorug02G0599200
rosa_samantha	Rh2AG676000 Rh2BG687100 Rh2CG650500
rosa_wichuraiana	Rw2G055470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	33
AccB1I	GGYRCC	2 cut(s)	203, 1706
AccI	GTMKAC	1 cut(s)	561
AccII	CGCG	3 cut(s)	500, 1543, 1552
AccIII	TCCGGA	1 cut(s)	2148
AciI	CCGC	9 cut(s)	213, 304, 433, 498, 500, 618, 906, 1507, 1552
AclI	AACGTT	1 cut(s)	753
AclWI	GGATC	3 cut(s)	1187, 1358, 2318
AcoI	YGGCCR	2 cut(s)	321, 1712
AcsI	RAATTY	1 cut(s)	112
AcuI	CTGAAG	1 cut(s)	2073
AdeI	CACNNNGTG	1 cut(s)	1662
AfaI	GTAC	6 cut(s)	734, 1102, 1274, 1333, 2008, 2130
AfiI	CCNNNNNNNGG	9 cut(s)	198, 455, 693, 1041, 1042, 1439, 1703, 1748, 1901
AflIII	ACRYGT	1 cut(s)	1163
AgsI	TTSAA	9 cut(s)	395, 613, 662, 778, 1028, 1415, 1621, 1638, 1793
AhlI	ACTAGT	2 cut(s)	233, 1214
AjnI	CCWGG	9 cut(s)	191, 435, 709, 1438, 1709, 1723, 1834, 1900, 2221
AjuI	GAANNNNNNNTTGG	2 cut(s)	654, 686
AleI	CACNNNNGTG	1 cut(s)	1502
Alw21I	GWGCWC	4 cut(s)	90, 233, 865, 1226
AlwI	GGATC	3 cut(s)	1187, 1358, 2318
Aor13HI	TCCGGA	1 cut(s)	2148
AoxI	GGCC	6 cut(s)	321, 550, 1712, 1832, 2217, 2224
ApaI	GGGCCC	1 cut(s)	2221
ApeKI	GCWGC	6 cut(s)	216, 219, 1016, 1929, 2057, 2195
ApoI	RAATTY	1 cut(s)	112
AseI	ATTAAT	1 cut(s)	1593
AspLEI	GCGC	1 cut(s)	341
AspS9I	GGNCC	8 cut(s)	483, 493, 550, 1379, 1509, 1567, 2217, 2218
AsuC2I	CCSGG	2 cut(s)	456, 1704
AsuHPI	GGTGA	6 cut(s)	259, 616, 686, 812, 1931, 2315
AvaII	GGWCC	5 cut(s)	483, 493, 1379, 1509, 1567
BaeGI	GKGCMC	3 cut(s)	206, 1362, 2221
BalI	TGGCCA	2 cut(s)	323, 1714
BanI	GGYRCC	2 cut(s)	203, 1706
BanII	GRGCYC	3 cut(s)	90, 1423, 2221
Bbv12I	GWGCWC	4 cut(s)	90, 233, 865, 1226
BbvI	GCAGC	6 cut(s)	228, 231, 1003, 1941, 2044, 2207
BccI	CCATC	6 cut(s)	374, 494, 1321, 1605, 1916, 1937
BcgI	CGANNNNNNTGC	2 cut(s)	864, 898
BciT130I	CCWGG	9 cut(s)	193, 437, 711, 1440, 1711, 1725, 1836, 1902, 2223
BclI	TGATCA	1 cut(s)	1861
BcnI	CCSGG	2 cut(s)	456, 1704
BcuI	ACTAGT	2 cut(s)	233, 1214
BfaI	CTAG	4 cut(s)	180, 234, 1215, 2283
BfmI	CTRYAG	1 cut(s)	1050
BfoI	RGCGCY	1 cut(s)	342
BglII	AGATCT	1 cut(s)	176
BisI	GCNGC	8 cut(s)	214, 217, 220, 305, 1017, 1930, 2058, 2196
BlsI	GCNGC	8 cut(s)	215, 218, 221, 306, 1018, 1931, 2059, 2197
Bme18I	GGWCC	5 cut(s)	483, 493, 1379, 1509, 1567
BmgT120I	GGNCC	8 cut(s)	483, 493, 550, 1379, 1509, 1567, 2217, 2218
BmrI	ACTGGG	5 cut(s)	703, 1236, 1365, 2038, 2224
BmsI	GCATC	5 cut(s)	709, 793, 1003, 1174, 1916
BmuI	ACTGGG	5 cut(s)	703, 1236, 1365, 2038, 2224
BplI	GAGNNNNNCTC	2 cut(s)	369, 401
BpmI	CTGGAG	3 cut(s)	488, 1278, 1857
BpuEI	CTTGAG	1 cut(s)	74
BpuMI	CCSGG	2 cut(s)	456, 1704
BsaBI	GATNNNNATC	2 cut(s)	187, 1947
BsaJI	CCNNGG	7 cut(s)	192, 318, 498, 1570, 1900, 1901, 2221
BsaWI	WCCGGW	3 cut(s)	58, 2148, 2200
BsaXI	ACNNNNNCTCC	2 cut(s)	118, 148
Bsc4I	CCNNNNNNNGG	9 cut(s)	198, 455, 693, 1041, 1042, 1439, 1703, 1748, 1901
Bse3DI	GCAATG	2 cut(s)	709, 970
Bse8I	GATNNNNATC	2 cut(s)	187, 1947
BseAI	TCCGGA	1 cut(s)	2148
BseBI	CCWGG	9 cut(s)	193, 437, 711, 1440, 1711, 1725, 1836, 1902, 2223
BseDI	CCNNGG	7 cut(s)	192, 318, 498, 1570, 1900, 1901, 2221
BseGI	GGATG	7 cut(s)	735, 990, 1031, 1144, 1777, 1931, 2038
BseJI	GATNNNNATC	2 cut(s)	187, 1947
BseLI	CCNNNNNNNGG	9 cut(s)	198, 455, 693, 1041, 1042, 1439, 1703, 1748, 1901
BseMI	GCAATG	2 cut(s)	709, 970
BseMII	CTCAG	1 cut(s)	2258
BseRI	GAGGAG	5 cut(s)	45, 120, 123, 126, 2272
BseSI	GKGCMC	3 cut(s)	206, 1362, 2221
BseXI	GCAGC	6 cut(s)	228, 231, 1003, 1941, 2044, 2207
BseYI	CCCAGC	1 cut(s)	416
BsgI	GTGCAG	3 cut(s)	894, 1188, 1822
Bsh1236I	CGCG	3 cut(s)	500, 1543, 1552
BshFI	GGCC	6 cut(s)	323, 552, 1714, 1834, 2219, 2226
BshNI	GGYRCC	2 cut(s)	203, 1706
BsiHKAI	GWGCWC	4 cut(s)	90, 233, 865, 1226
BsiSI	CCGG	5 cut(s)	59, 456, 1703, 2149, 2201
BslFI	GGGAC	2 cut(s)	1495, 1949
BslI	CCNNNNNNNGG	9 cut(s)	198, 455, 693, 1041, 1042, 1439, 1703, 1748, 1901
BsmFI	GGGAC	2 cut(s)	1495, 1949
BsmI	GAATGC	1 cut(s)	1841
BsnI	GGCC	6 cut(s)	323, 552, 1714, 1834, 2219, 2226
Bsp120I	GGGCCC	1 cut(s)	2217
Bsp1286I	GDGCHC	8 cut(s)	90, 206, 233, 865, 1226, 1362, 1423, 2221
Bsp13I	TCCGGA	1 cut(s)	2148
Bsp1407I	TGTACA	2 cut(s)	1272, 1331
Bsp143I	GATC	8 cut(s)	176, 403, 1192, 1350, 1861, 2181, 2323, 2338
Bsp19I	CCATGG	1 cut(s)	318
BspACI	CCGC	9 cut(s)	213, 304, 433, 498, 500, 618, 906, 1507, 1552
BspANI	GGCC	6 cut(s)	323, 552, 1714, 1834, 2219, 2226
BspCNI	CTCAG	1 cut(s)	2259
BspEI	TCCGGA	1 cut(s)	2148
BspFNI	CGCG	3 cut(s)	500, 1543, 1552
BspHI	TCATGA	1 cut(s)	1864
BspPI	GGATC	3 cut(s)	1187, 1358, 2318
BspT107I	GGYRCC	2 cut(s)	203, 1706
BsrDI	GCAATG	2 cut(s)	709, 970
BsrGI	TGTACA	2 cut(s)	1272, 1331
BssECI	CCNNGG	7 cut(s)	192, 318, 498, 1570, 1900, 1901, 2221
BssMI	GATC	8 cut(s)	176, 403, 1192, 1350, 1861, 2181, 2323, 2338
BssT1I	CCWWGG	2 cut(s)	318, 1570
Bst2UI	CCWGG	9 cut(s)	193, 437, 711, 1440, 1711, 1725, 1836, 1902, 2223
Bst4CI	ACNGT	3 cut(s)	1343, 1859, 2289
Bst6I	CTCTTC	1 cut(s)	390
BstAUI	TGTACA	2 cut(s)	1272, 1331
BstC8I	GCNNGC	5 cut(s)	761, 765, 855, 865, 1586
BstDEI	CTNAG	3 cut(s)	140, 984, 2267
BstDSI	CCRYGG	2 cut(s)	318, 498
BstF5I	GGATG	7 cut(s)	735, 990, 1031, 1144, 1777, 1931, 2038
BstFNI	CGCG	3 cut(s)	500, 1543, 1552
BstH2I	RGCGCY	1 cut(s)	342
BstHHI	GCGC	1 cut(s)	341
BstKTI	GATC	8 cut(s)	179, 406, 1195, 1353, 1864, 2184, 2326, 2341
BstMBI	GATC	8 cut(s)	176, 403, 1192, 1350, 1861, 2181, 2323, 2338
BstMWI	GCNNNNNNNGC	8 cut(s)	210, 219, 228, 642, 1549, 1720, 2057, 2195
BstNI	CCWGG	9 cut(s)	193, 437, 711, 1440, 1711, 1725, 1836, 1902, 2223
BstNSI	RCATGY	2 cut(s)	311, 1167
BstSFI	CTRYAG	1 cut(s)	1050
BstSLI	GKGCMC	3 cut(s)	206, 1362, 2221
BstUI	CGCG	3 cut(s)	500, 1543, 1552
BstV1I	GCAGC	6 cut(s)	228, 231, 1003, 1941, 2044, 2207
BstX2I	RGATCY	3 cut(s)	176, 1192, 2323
BstXI	CCANNNNNNTGG	3 cut(s)	373, 1256, 1605
BstYI	RGATCY	3 cut(s)	176, 1192, 2323
BsuRI	GGCC	6 cut(s)	323, 552, 1714, 1834, 2219, 2226
BtgI	CCRYGG	2 cut(s)	318, 498
BtsCI	GGATG	7 cut(s)	735, 990, 1031, 1144, 1777, 1931, 2038
BtsI	GCAGTG	3 cut(s)	1176, 1275, 1810
BtsIMutI	CAGTG	9 cut(s)	1176, 1224, 1275, 1353, 1810, 1951, 1996, 2051, 2212
Cac8I	GCNNGC	5 cut(s)	761, 765, 855, 865, 1586
CciI	TCATGA	1 cut(s)	1864
CfoI	GCGC	1 cut(s)	341
Cfr13I	GGNCC	8 cut(s)	483, 493, 550, 1379, 1509, 1567, 2217, 2218
Cfr42I	CCGCGG	1 cut(s)	501
CseI	GACGC	2 cut(s)	1386, 1452
Csp6I	GTAC	6 cut(s)	733, 1101, 1273, 1332, 2007, 2129
CspCI	CAANNNNNGTGG	4 cut(s)	737, 772, 1972, 2007
CviQI	GTAC	6 cut(s)	733, 1101, 1273, 1332, 2007, 2129
DdeI	CTNAG	3 cut(s)	140, 984, 2267
DpnI	GATC	8 cut(s)	178, 405, 1194, 1352, 1863, 2183, 2325, 2340
DpnII	GATC	8 cut(s)	176, 403, 1192, 1350, 1861, 2181, 2323, 2338
DraI	TTTAAA	3 cut(s)	187, 1291, 1891
DraIII	CACNNNGTG	1 cut(s)	1662
EaeI	YGGCCR	2 cut(s)	321, 1712
Eam1104I	CTCTTC	1 cut(s)	390
EarI	CTCTTC	1 cut(s)	390
Ecl136II	GAGCTC	1 cut(s)	88
Eco130I	CCWWGG	2 cut(s)	318, 1570
Eco24I	GRGCYC	3 cut(s)	90, 1423, 2221
Eco32I	GATATC	2 cut(s)	595, 1949
Eco47I	GGWCC	5 cut(s)	483, 493, 1379, 1509, 1567
Eco53kI	GAGCTC	1 cut(s)	88
Eco57I	CTGAAG	1 cut(s)	2073
EcoICRI	GAGCTC	1 cut(s)	88
EcoO109I	RGGNCCY	3 cut(s)	493, 1567, 2218
EcoRII	CCWGG	9 cut(s)	191, 435, 709, 1438, 1709, 1723, 1834, 1900, 2221
EcoRV	GATATC	2 cut(s)	595, 1949
EcoT14I	CCWWGG	2 cut(s)	318, 1570
EcoT22I	ATGCAT	1 cut(s)	1300
EcoT38I	GRGCYC	3 cut(s)	90, 1423, 2221
ErhI	CCWWGG	2 cut(s)	318, 1570
FalI	AAGNNNNNCTT	4 cut(s)	654, 686, 1182, 1214
FaqI	GGGAC	2 cut(s)	1495, 1949
FauI	CCCGC	3 cut(s)	440, 493, 505
FauNDI	CATATG	1 cut(s)	1375
FbaI	TGATCA	1 cut(s)	1861
FblI	GTMKAC	1 cut(s)	561
Fnu4HI	GCNGC	8 cut(s)	214, 217, 220, 305, 1017, 1930, 2058, 2196
FokI	GGATG	7 cut(s)	722, 977, 1018, 1151, 1784, 1938, 2025
FriOI	GRGCYC	3 cut(s)	90, 1423, 2221
Fsp4HI	GCNGC	8 cut(s)	214, 217, 220, 305, 1017, 1930, 2058, 2196
FspBI	CTAG	4 cut(s)	180, 234, 1215, 2283
GlaI	GCGC	1 cut(s)	340
GluI	GCNGC	8 cut(s)	214, 217, 220, 305, 1017, 1930, 2058, 2196
GsaI	CCCAGC	1 cut(s)	420
GsuI	CTGGAG	3 cut(s)	488, 1278, 1857
HaeII	RGCGCY	1 cut(s)	342
HaeIII	GGCC	6 cut(s)	323, 552, 1714, 1834, 2219, 2226
HapII	CCGG	5 cut(s)	59, 456, 1703, 2149, 2201
HgaI	GACGC	2 cut(s)	1386, 1452
HhaI	GCGC	1 cut(s)	341
Hin6I	GCGC	1 cut(s)	339
HinP1I	GCGC	1 cut(s)	339
HinfI	GANTC	9 cut(s)	263, 314, 571, 650, 911, 1028, 1634, 2021, 2263
HpaII	CCGG	5 cut(s)	59, 456, 1703, 2149, 2201
HphI	GGTGA	6 cut(s)	259, 616, 686, 812, 1931, 2315
Hpy188I	TCNGA	9 cut(s)	85, 514, 599, 1318, 1654, 1847, 2174, 2186, 2268
Hpy188III	TCNNGA	8 cut(s)	91, 180, 467, 575, 583, 1865, 2149, 2283
Hpy99I	CGWCG	2 cut(s)	79, 425
HpyAV	CCTTC	8 cut(s)	342, 692, 1087, 1231, 1409, 1771, 1787, 2237
HpyCH4III	ACNGT	3 cut(s)	1343, 1859, 2289
HpyCH4IV	ACGT	3 cut(s)	74, 731, 753
HpyF10VI	GCNNNNNNNGC	8 cut(s)	210, 219, 228, 642, 1549, 1720, 2057, 2195
HpyF3I	CTNAG	3 cut(s)	140, 984, 2267
HpySE526I	ACGT	3 cut(s)	74, 731, 753
HspAI	GCGC	1 cut(s)	339
Kpn2I	TCCGGA	1 cut(s)	2148
Ksp22I	TGATCA	1 cut(s)	1861
KspI	CCGCGG	1 cut(s)	501
Kzo9I	GATC	8 cut(s)	176, 403, 1192, 1350, 1861, 2181, 2323, 2338
LmnI	GCTCC	5 cut(s)	1221, 1394, 1426, 1838, 2186
Lsp1109I	GCAGC	6 cut(s)	228, 231, 1003, 1941, 2044, 2207
LweI	GCATC	5 cut(s)	709, 793, 1003, 1174, 1916
MaeI	CTAG	4 cut(s)	180, 234, 1215, 2283
MaeII	ACGT	3 cut(s)	74, 731, 753
MaeIII	GTNAC	7 cut(s)	674, 1369, 1474, 1526, 1537, 2110, 2303
MalI	GATC	8 cut(s)	178, 405, 1194, 1352, 1863, 2183, 2325, 2340
MboI	GATC	8 cut(s)	176, 403, 1192, 1350, 1861, 2181, 2323, 2338
MfeI	CAATTG	2 cut(s)	640, 1079
MflI	RGATCY	3 cut(s)	176, 1192, 2323
MhlI	GDGCHC	8 cut(s)	90, 206, 233, 865, 1226, 1362, 1423, 2221
MlsI	TGGCCA	2 cut(s)	323, 1714
MluNI	TGGCCA	2 cut(s)	323, 1714
MlyI	GAGTC	2 cut(s)	323, 644
MmeI	TCCRAC	4 cut(s)	492, 1181, 1328, 1609
Mox20I	TGGCCA	2 cut(s)	323, 1714
Mph1103I	ATGCAT	1 cut(s)	1300
MroI	TCCGGA	1 cut(s)	2148
MscI	TGGCCA	2 cut(s)	323, 1714
MslI	CAYNNNNRTG	3 cut(s)	1365, 1502, 1609
Msp20I	TGGCCA	2 cut(s)	323, 1714
MspA1I	CMGCKG	3 cut(s)	500, 763, 1337
MspI	CCGG	5 cut(s)	59, 456, 1703, 2149, 2201
MunI	CAATTG	2 cut(s)	640, 1079
Mva1269I	GAATGC	1 cut(s)	1841
MvaI	CCWGG	9 cut(s)	193, 437, 711, 1440, 1711, 1725, 1836, 1902, 2223
MvnI	CGCG	3 cut(s)	500, 1543, 1552
MwoI	GCNNNNNNNGC	8 cut(s)	210, 219, 228, 642, 1549, 1720, 2057, 2195
NciI	CCSGG	2 cut(s)	456, 1704
NcoI	CCATGG	1 cut(s)	318
NdeI	CATATG	1 cut(s)	1375
NdeII	GATC	8 cut(s)	176, 403, 1192, 1350, 1861, 2181, 2323, 2338
NmuCI	GTSAC	5 cut(s)	674, 1369, 1474, 1526, 2303
NsiI	ATGCAT	1 cut(s)	1300
NspI	RCATGY	2 cut(s)	311, 1167
OliI	CACNNNNGTG	1 cut(s)	1502
PagI	TCATGA	1 cut(s)	1864
PasI	CCCWGGG	1 cut(s)	1901
PciI	ACATGT	1 cut(s)	1163
PctI	GAATGC	1 cut(s)	1841
PfeI	GAWTC	7 cut(s)	263, 571, 911, 1028, 1634, 2021, 2263
PflFI	GACNNNGTC	1 cut(s)	2207
PfoI	TCCNGGA	1 cut(s)	1438
PkrI	GCNGC	8 cut(s)	215, 218, 221, 306, 1018, 1931, 2059, 2197
PleI	GAGTC	2 cut(s)	322, 644
PpsI	GAGTC	2 cut(s)	322, 644
PpuMI	RGGWCCY	2 cut(s)	493, 1567
PscI	ACATGT	1 cut(s)	1163
PshBI	ATTAAT	1 cut(s)	1593
PsiI	TTATAA	1 cut(s)	33
Psp124BI	GAGCTC	1 cut(s)	90
Psp1406I	AACGTT	1 cut(s)	753
Psp5II	RGGWCCY	2 cut(s)	493, 1567
Psp6I	CCWGG	9 cut(s)	191, 435, 709, 1438, 1709, 1723, 1834, 1900, 2221
PspFI	CCCAGC	1 cut(s)	416
PspGI	CCWGG	9 cut(s)	191, 435, 709, 1438, 1709, 1723, 1834, 1900, 2221
PspOMI	GGGCCC	1 cut(s)	2217
PspPI	GGNCC	8 cut(s)	483, 493, 550, 1379, 1509, 1567, 2217, 2218
PspPPI	RGGWCCY	2 cut(s)	493, 1567
PsuI	RGATCY	3 cut(s)	176, 1192, 2323
PsyI	GACNNNGTC	1 cut(s)	2207
PvuII	CAGCTG	2 cut(s)	763, 1337
RsaI	GTAC	6 cut(s)	734, 1102, 1274, 1333, 2008, 2130
RsaNI	GTAC	6 cut(s)	733, 1101, 1273, 1332, 2007, 2129
RseI	CAYNNNNRTG	3 cut(s)	1365, 1502, 1609
SacI	GAGCTC	1 cut(s)	90
SacII	CCGCGG	1 cut(s)	501
SatI	GCNGC	8 cut(s)	214, 217, 220, 305, 1017, 1930, 2058, 2196
Sau3AI	GATC	8 cut(s)	176, 403, 1192, 1350, 1861, 2181, 2323, 2338
Sau96I	GGNCC	8 cut(s)	483, 493, 550, 1379, 1509, 1567, 2217, 2218
SchI	GAGTC	2 cut(s)	323, 644
SduI	GDGCHC	8 cut(s)	90, 206, 233, 865, 1226, 1362, 1423, 2221
SfaNI	GCATC	5 cut(s)	709, 793, 1003, 1174, 1916
SfcI	CTRYAG	1 cut(s)	1050
Sfr303I	CCGCGG	1 cut(s)	501
SgrBI	CCGCGG	1 cut(s)	501
SinI	GGWCC	5 cut(s)	483, 493, 1379, 1509, 1567
SmiI	ATTTAAAT	2 cut(s)	187, 1291
SmiMI	CAYNNNNRTG	3 cut(s)	1365, 1502, 1609
SmlI	CTYRAG	1 cut(s)	89
SmoI	CTYRAG	1 cut(s)	89
SpeI	ACTAGT	2 cut(s)	233, 1214
SsiI	CCGC	9 cut(s)	213, 304, 433, 498, 500, 618, 906, 1507, 1552
SspI	AATATT	1 cut(s)	14
SspMI	CTAG	4 cut(s)	180, 234, 1215, 2283
SstI	GAGCTC	1 cut(s)	90
StyI	CCWWGG	2 cut(s)	318, 1570
SwaI	ATTTAAAT	2 cut(s)	187, 1291
TaaI	ACNGT	3 cut(s)	1343, 1859, 2289
TaiI	ACGT	3 cut(s)	77, 734, 756
TaqI	TCGA	3 cut(s)	489, 576, 2337
TatI	WGTACW	2 cut(s)	1272, 1331
TauI	GCSGC	2 cut(s)	216, 307
TfiI	GAWTC	7 cut(s)	263, 571, 911, 1028, 1634, 2021, 2263
TscAI	CASTG	9 cut(s)	1176, 1231, 1282, 1360, 1810, 1958, 1996, 2051, 2219
TseFI	GTSAC	5 cut(s)	674, 1369, 1474, 1526, 2303
TseI	GCWGC	6 cut(s)	216, 219, 1016, 1929, 2057, 2195
Tsp45I	GTSAC	5 cut(s)	674, 1369, 1474, 1526, 2303
TspDTI	ATGAA	3 cut(s)	1086, 1103, 1881
TspRI	CASTG	9 cut(s)	1176, 1231, 1282, 1360, 1810, 1958, 1996, 2051, 2219
Tth111I	GACNNNGTC	1 cut(s)	2207
VpaK11BI	GGWCC	5 cut(s)	483, 493, 1379, 1509, 1567
VspI	ATTAAT	1 cut(s)	1593
XapI	RAATTY	1 cut(s)	112
XbaI	TCTAGA	2 cut(s)	179, 2282
XceI	RCATGY	2 cut(s)	311, 1167
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2219
XmiI	GTMKAC	1 cut(s)	561
XspI	CTAG	4 cut(s)	180, 234, 1215, 2283
Zsp2I	ATGCAT	1 cut(s)	1300

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.