Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003523

Physical Location & Seq

Genomic Coordinates Reverse (-)

559746 .. 563210

Gene Structure 3465 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1008100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1332 bp

ATGGATGATGAGTACGCCAAGTTCGTCAGGAGGATGAACCCACCGAGAGTTGTGATCGATAACGATTATTGCGAGGATGCCACCGTTATCCAGGTTGACAGTGTCAACAAACATGGAATTCTGCTGGATGTTGTCCAAGTGCTCGCCGACGTGAACCTTGTAATAACCAAAGCTTATATCTCATCTGATGGTGTATGGTTCATGGATGTGTTTAATGTGATTGACCGTGAAGGGAAAAAAATCAGAGACAAGGACATTATCAATTATATTCAAATGAGACTTGAAAGCAATGGTGGCTTTGTACCCTCTATGAGAGATTCCGTTGGCATGATGCCCTCAGAAGAGTACACCTCCATTGAGCTCAGTGGTACCGACAGGCCTGGCTTATTGTCTGAAGTGTGTGCGGTTCTTGCAGACCTTCACTGTAATGTGGTAAATGCTGAGATATGGACACACAACGCTAGAGCTGCAGCTGTAGTTCATGTCACAGATGATTCCACTGGCTGCGCAATTGAAGATCCAATTCGTCTCGCTACAATTAAGGAGTTGCTTTGCAATGTCCTCAAAGTAAACAGTGAGTTGAAGGCTGCTAAAATGACACTTGCAACCCCTGGAGTTACAAACAGAGATAGAAGGTTGCATCAGATTATGTTTGCTGACAGGGATTATGAAAGGGTTGAAAGAGCAGAACTAAGGAGAGTTGAGGATAAGGGCTCAAAACCCCATGTAACCGCGTTGGATTGCAGCCAGAAGGATTACACTGTCATTACCATGAGGTCAAAAGATCGACCAAAGTTGTTATTTGACATCATCTGCACTTTAACAGACATGCAATATGTGGTTTTTCACGGAATGGTCAATACAGGGAGGACGGAAGCTTATCAGGAATTTTACATTCGACATGTTGATGGGCTTCCTATCAACTCAGAAGCTGAGCGAGAACGTGTTGTACAGTGCCTTGAAGCAGCCATTGAAAGGCGGGCATCTGAGGGCCTGGAGCTACAACTGTGCACAGATGACCGGGTCGGACTTCTCTCAGATATCACCCGGATATTCAGAGAGAACAGTTTGCGTGTCACACGAGCAGAAATTTCAACTAAAAGTGGAAAAGCAATAGACACATTCTATGTCACGGACGTTACAGGAAGCCCAGTAGACCCCAAGATTATCGATTCAGTTCGCAGACAGATTGGGCAGACCATACTGCAGGTGAAGTGTAACTCAAGCCCTGCACCAAAGCCTCCTCAAGGGACAACAATGGGATATCTCTTCGGAAACTTATTCAAAGCTCGAAGTTTCCAGCCCCTTAAGTTGGTTCGATCCTACTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0006355 GO:0006464 GO:0006807 GO:0006808 GO:0007154 GO:0007584 GO:0008150 GO:0008152 GO:0009605 GO:0009719 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016597 GO:0018175 GO:0018177 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031406 GO:0031667 GO:0031668 GO:0031669 GO:0031670 GO:0036094 GO:0036211 GO:0042221 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071417 GO:0071495 GO:0071496 GO:0071704 GO:0080090 GO:0090293 GO:1901564 GO:1901698 GO:1901699 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACT ACT_6

Protein Analysis

444

Amino Acids

49.9

Weight (kDa)

5.86

Isoelectric Point (pI)

37.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACT_ACR9_3rd	PF24931	17 - 96	2.8e-07	ACR9-like, ACT-like domain
ACR10_N	PF24914	25 - 81	2e-06	ACT domain-containing protein ACR10, N-terminal
ACT	PF01842	28 - 77	9e-07	ACT domain
ACT	PF01842	117 - 165	8.5e-10	ACT domain
ACT	PF01842	339 - 395	2.6e-07	ACT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003523)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01990 AT3G01990 AT3G01990 AT3G01990 AT3G01990 AT3G01990 AT3G01990 AT3G01990 AT3G01990 AT3G01990 AT3G01990
fragaria_vesca	FvH4_6g53840
malus_domestica	MD09G1008100.v1.1 MD17G1002600.v1.1
prunus_persica	Prupe.3G308800_v2.0.a1 Prupe.3G308800_v2.0.a1 Prupe.3G308800_v2.0.a1 Prupe.3G308800_v2.0.a1 Prupe.3G308800_v2.0.a1 Prupe.3G308800_v2.0.a1
pyrus_communis	pycom111g00650 pycom17g00680
rosa_chinensis	RchiOBHm_Chr2g0175981
rosa_laevigata	RLG00000022370
rosa_multiflora	Rmu_co8433007.1_g000001 Rmu_sc0012513.1_g000007 Rmu_sc0018801.1_g000001
rosa_roxburghii	Rroxscaffold_2G00076890
rosa_rugosa	Rorug02G0589100 Rorug02G0589100
rosa_samantha	Rh2AG668300 Rh2BG679800 Rh2CG642700 Rh2DG693500
rosa_wichuraiana	Rw2G054790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1198
Acc16I	TGCGCA	1 cut(s)	508
Acc36I	ACCTGC	1 cut(s)	1198
Acc65I	GGTACC	1 cut(s)	368
AccB1I	GGYRCC	1 cut(s)	368
AccI	GTMKAC	1 cut(s)	1155
AccII	CGCG	1 cut(s)	734
AciI	CCGC	3 cut(s)	404, 732, 979
AclWI	GGATC	2 cut(s)	512, 1314
AcsI	RAATTY	3 cut(s)	117, 887, 1089
AcuI	CTGAAG	1 cut(s)	414
AfaI	GTAC	5 cut(s)	14, 303, 347, 370, 951
AfiI	CCNNNNNNNGG	3 cut(s)	975, 1247, 1312
AflII	CTTAAG	1 cut(s)	1307
AflIII	ACRYGT	2 cut(s)	901, 943
AgsI	TTSAA	9 cut(s)	272, 284, 515, 583, 680, 962, 974, 1095, 1285
AjiI	CACGTC	1 cut(s)	151
AjnI	CCWGG	4 cut(s)	90, 379, 610, 993
AluBI	AGCT	8 cut(s)	173, 361, 467, 473, 878, 932, 1000, 1289
AluI	AGCT	8 cut(s)	173, 361, 467, 473, 878, 932, 1000, 1289
Alw21I	GWGCWC	3 cut(s)	144, 363, 1013
Alw26I	GTCTC	3 cut(s)	240, 271, 533
Alw44I	GTGCAC	1 cut(s)	1009
AlwI	GGATC	2 cut(s)	512, 1314
AlwNI	CAGNNNCTG	1 cut(s)	932
AoxI	GGCC	2 cut(s)	377, 991
ApaLI	GTGCAC	1 cut(s)	1009
ApeKI	GCWGC	6 cut(s)	467, 470, 504, 587, 744, 965
ApoI	RAATTY	3 cut(s)	117, 887, 1089
Asp718I	GGTACC	1 cut(s)	368
AspLEI	GCGC	1 cut(s)	509
AspS9I	GGNCC	1 cut(s)	991
AsuC2I	CCSGG	2 cut(s)	1022, 1048
AsuHPI	GGTGA	2 cut(s)	1036, 1222
BaeGI	GKGCMC	1 cut(s)	1013
BanI	GGYRCC	1 cut(s)	368
BanII	GRGCYC	2 cut(s)	363, 716
BauI	CACGAG	1 cut(s)	1080
Bbv12I	GWGCWC	3 cut(s)	144, 363, 1013
BbvI	GCAGC	6 cut(s)	454, 482, 491, 574, 756, 977
BccI	CCATC	2 cut(s)	182, 902
BciT130I	CCWGG	4 cut(s)	92, 381, 612, 995
BcnI	CCSGG	2 cut(s)	1022, 1048
BcoDI	GTCTC	3 cut(s)	240, 271, 533
BfaI	CTAG	1 cut(s)	462
BfmI	CTRYAG	3 cut(s)	468, 474, 1205
BfrI	CTTAAG	1 cut(s)	1307
BfuAI	ACCTGC	1 cut(s)	1198
BisI	GCNGC	6 cut(s)	468, 471, 505, 588, 745, 966
BlpI	GCTNAGC	1 cut(s)	933
BlsI	GCNGC	6 cut(s)	469, 472, 506, 589, 746, 967
Bme1390I	CCNGG	6 cut(s)	92, 381, 612, 995, 1022, 1048
BmgBI	CACGTC	1 cut(s)	151
BmgT120I	GGNCC	1 cut(s)	991
BmiI	GGNNCC	1 cut(s)	370
BmrFI	CCNGG	6 cut(s)	92, 381, 612, 995, 1022, 1048
BmrI	ACTGGG	1 cut(s)	1145
BmsI	GCATC	4 cut(s)	67, 321, 649, 992
BmuI	ACTGGG	1 cut(s)	1145
BplI	GAGNNNNNCTC	2 cut(s)	335, 367
BpmI	CTGGAG	2 cut(s)	633, 1016
Bpu1102I	GCTNAGC	1 cut(s)	933
BpuEI	CTTGAG	2 cut(s)	1207, 1230
BpuMI	CCSGG	2 cut(s)	1022, 1048
Bsa29I	ATCGAT	2 cut(s)	57, 1170
BsaJI	CCNNGG	1 cut(s)	610
Bsc4I	CCNNNNNNNGG	3 cut(s)	975, 1247, 1312
Bse1I	ACTGG	2 cut(s)	505, 1151
Bse3DI	GCAATG	2 cut(s)	295, 562
BseBI	CCWGG	4 cut(s)	92, 381, 612, 995
BseCI	ATCGAT	2 cut(s)	57, 1170
BseDI	CCNNGG	1 cut(s)	610
BseGI	GGATG	5 cut(s)	10, 39, 82, 133, 211
BseLI	CCNNNNNNNGG	3 cut(s)	975, 1247, 1312
BseMI	GCAATG	2 cut(s)	295, 562
BseMII	CTCAG	7 cut(s)	351, 376, 432, 924, 939, 978, 1050
BseNI	ACTGG	2 cut(s)	505, 1151
BseRI	GAGGAG	1 cut(s)	1233
BseSI	GKGCMC	1 cut(s)	1013
BseXI	GCAGC	6 cut(s)	454, 482, 491, 574, 756, 977
BsgI	GTGCAG	2 cut(s)	799, 1215
Bsh1236I	CGCG	1 cut(s)	734
BshFI	GGCC	2 cut(s)	379, 993
BshNI	GGYRCC	1 cut(s)	368
BshVI	ATCGAT	2 cut(s)	57, 1170
BsiHKAI	GWGCWC	3 cut(s)	144, 363, 1013
BsiSI	CCGG	2 cut(s)	1021, 1048
BslFI	GGGAC	1 cut(s)	1264
BslI	CCNNNNNNNGG	3 cut(s)	975, 1247, 1312
BsmAI	GTCTC	3 cut(s)	240, 271, 533
BsmBI	CGTCTC	1 cut(s)	533
BsmFI	GGGAC	1 cut(s)	1264
BsnI	GGCC	2 cut(s)	379, 993
Bsp1286I	GDGCHC	4 cut(s)	144, 363, 716, 1013
Bsp1407I	TGTACA	1 cut(s)	949
Bsp143I	GATC	4 cut(s)	54, 517, 784, 1319
Bsp1720I	GCTNAGC	1 cut(s)	933
BspACI	CCGC	3 cut(s)	404, 732, 979
BspANI	GGCC	2 cut(s)	379, 993
BspCNI	CTCAG	7 cut(s)	350, 375, 433, 925, 938, 979, 1049
BspDI	ATCGAT	2 cut(s)	57, 1170
BspFNI	CGCG	1 cut(s)	734
BspLI	GGNNCC	1 cut(s)	370
BspMAI	CTGCAG	2 cut(s)	472, 1209
BspMI	ACCTGC	1 cut(s)	1198
BspPI	GGATC	2 cut(s)	512, 1314
BspT107I	GGYRCC	1 cut(s)	368
BspTI	CTTAAG	1 cut(s)	1307
BsrDI	GCAATG	2 cut(s)	295, 562
BsrGI	TGTACA	1 cut(s)	949
BsrI	ACTGG	2 cut(s)	505, 1151
BssECI	CCNNGG	1 cut(s)	610
BssMI	GATC	4 cut(s)	54, 517, 784, 1319
BssSI	CACGAG	1 cut(s)	1080
Bst2BI	CACGAG	1 cut(s)	1080
Bst2UI	CCWGG	4 cut(s)	92, 381, 612, 995
Bst4CI	ACNGT	9 cut(s)	85, 101, 227, 425, 575, 763, 954, 1008, 1067
Bst6I	CTCTTC	2 cut(s)	336, 1274
BstAFI	CTTAAG	1 cut(s)	1307
BstAUI	TGTACA	1 cut(s)	949
BstC8I	GCNNGC	2 cut(s)	144, 981
BstDEI	CTNAG	9 cut(s)	337, 362, 441, 692, 925, 933, 987, 1036, 1329
BstENI	CCTNNNNNAGG	1 cut(s)	1245
BstF5I	GGATG	5 cut(s)	10, 39, 82, 133, 211
BstFNI	CGCG	1 cut(s)	734
BstHHI	GCGC	1 cut(s)	509
BstKTI	GATC	4 cut(s)	57, 520, 787, 1322
BstMAI	GTCTC	3 cut(s)	240, 271, 533
BstMBI	GATC	4 cut(s)	54, 517, 784, 1319
BstMWI	GCNNNNNNNGC	3 cut(s)	294, 410, 467
BstNI	CCWGG	4 cut(s)	92, 381, 612, 995
BstNSI	RCATGY	2 cut(s)	832, 905
BstSCI	CCNGG	6 cut(s)	90, 379, 610, 993, 1020, 1046
BstSFI	CTRYAG	3 cut(s)	468, 474, 1205
BstSLI	GKGCMC	1 cut(s)	1013
BstUI	CGCG	1 cut(s)	734
BstV1I	GCAGC	6 cut(s)	454, 482, 491, 574, 756, 977
BstX2I	RGATCY	1 cut(s)	517
BstYI	RGATCY	1 cut(s)	517
Bsu15I	ATCGAT	2 cut(s)	57, 1170
BsuRI	GGCC	2 cut(s)	379, 993
BsuTUI	ATCGAT	2 cut(s)	57, 1170
BtrI	CACGTC	1 cut(s)	151
BtsCI	GGATG	5 cut(s)	10, 39, 82, 133, 211
BtsIMutI	CAGTG	7 cut(s)	106, 370, 421, 498, 580, 759, 959
BveI	ACCTGC	1 cut(s)	1198
Cac8I	GCNNGC	2 cut(s)	144, 981
CaiI	CAGNNNCTG	1 cut(s)	932
CfoI	GCGC	1 cut(s)	509
Cfr13I	GGNCC	1 cut(s)	991
ClaI	ATCGAT	2 cut(s)	57, 1170
Csp6I	GTAC	5 cut(s)	13, 302, 346, 369, 950
CviAII	CATG	8 cut(s)	113, 202, 328, 482, 725, 772, 829, 902
CviQI	GTAC	5 cut(s)	13, 302, 346, 369, 950
DdeI	CTNAG	9 cut(s)	337, 362, 441, 692, 925, 933, 987, 1036, 1329
DpnI	GATC	4 cut(s)	56, 519, 786, 1321
DpnII	GATC	4 cut(s)	54, 517, 784, 1319
Eam1104I	CTCTTC	2 cut(s)	336, 1274
EarI	CTCTTC	2 cut(s)	336, 1274
Ecl136II	GAGCTC	1 cut(s)	361
Eco147I	AGGCCT	1 cut(s)	379
Eco24I	GRGCYC	2 cut(s)	363, 716
Eco32I	GATATC	2 cut(s)	1042, 1265
Eco53kI	GAGCTC	1 cut(s)	361
Eco57I	CTGAAG	1 cut(s)	414
EcoICRI	GAGCTC	1 cut(s)	361
EcoNI	CCTNNNNNAGG	1 cut(s)	1245
EcoO109I	RGGNCCY	1 cut(s)	991
EcoRI	GAATTC	1 cut(s)	117
EcoRII	CCWGG	4 cut(s)	90, 379, 610, 993
EcoRV	GATATC	2 cut(s)	1042, 1265
EcoT38I	GRGCYC	2 cut(s)	363, 716
Esp3I	CGTCTC	1 cut(s)	533
FaeI	CATG	8 cut(s)	116, 205, 331, 485, 728, 775, 832, 905
FaqI	GGGAC	1 cut(s)	1264
FatI	CATG	8 cut(s)	112, 201, 327, 481, 724, 771, 828, 901
FauI	CCCGC	1 cut(s)	972
FblI	GTMKAC	1 cut(s)	1155
Fnu4HI	GCNGC	6 cut(s)	468, 471, 505, 588, 745, 966
FokI	GGATG	5 cut(s)	17, 46, 89, 140, 218
FriOI	GRGCYC	2 cut(s)	363, 716
Fsp4HI	GCNGC	6 cut(s)	468, 471, 505, 588, 745, 966
FspBI	CTAG	1 cut(s)	462
FspI	TGCGCA	1 cut(s)	508
GlaI	GCGC	1 cut(s)	508
GluI	GCNGC	6 cut(s)	468, 471, 505, 588, 745, 966
GsuI	CTGGAG	2 cut(s)	633, 1016
HaeIII	GGCC	2 cut(s)	379, 993
HapII	CCGG	2 cut(s)	1021, 1048
HhaI	GCGC	1 cut(s)	509
Hin1II	CATG	8 cut(s)	116, 205, 331, 485, 728, 775, 832, 905
Hin6I	GCGC	1 cut(s)	507
HinP1I	GCGC	1 cut(s)	507
HincII	GTYRAC	2 cut(s)	97, 106
HindII	GTYRAC	2 cut(s)	97, 106
HindIII	AAGCTT	2 cut(s)	171, 876
HinfI	GANTC	3 cut(s)	317, 494, 1172
HpaII	CCGG	2 cut(s)	1021, 1048
HphI	GGTGA	2 cut(s)	1036, 1222
Hpy166II	GTNNAC	7 cut(s)	97, 106, 154, 348, 571, 1011, 1156
Hpy188III	TCNNGA	2 cut(s)	28, 884
Hpy8I	GTNNAC	7 cut(s)	97, 106, 154, 348, 571, 1011, 1156
Hpy99I	CGWCG	1 cut(s)	152
HpyAV	CCTTC	5 cut(s)	224, 428, 577, 627, 745
HpyCH4III	ACNGT	9 cut(s)	85, 101, 227, 425, 575, 763, 954, 1008, 1067
HpyCH4IV	ACGT	3 cut(s)	150, 943, 1137
HpyF10VI	GCNNNNNNNGC	3 cut(s)	294, 410, 467
HpyF3I	CTNAG	9 cut(s)	337, 362, 441, 692, 925, 933, 987, 1036, 1329
HpySE526I	ACGT	3 cut(s)	150, 943, 1137
Hsp92II	CATG	8 cut(s)	116, 205, 331, 485, 728, 775, 832, 905
HspAI	GCGC	1 cut(s)	507
KpnI	GGTACC	1 cut(s)	372
Kzo9I	GATC	4 cut(s)	54, 517, 784, 1319
LmnI	GCTCC	1 cut(s)	997
Lsp1109I	GCAGC	6 cut(s)	454, 482, 491, 574, 756, 977
LweI	GCATC	4 cut(s)	67, 321, 649, 992
MaeI	CTAG	1 cut(s)	462
MaeII	ACGT	3 cut(s)	150, 943, 1137
MaeIII	GTNAC	7 cut(s)	484, 616, 727, 1075, 1129, 1138, 1217
MalI	GATC	4 cut(s)	56, 519, 786, 1321
MboI	GATC	4 cut(s)	54, 517, 784, 1319
MboII	GAAGA	3 cut(s)	353, 527, 1261
MfeI	CAATTG	1 cut(s)	510
MflI	RGATCY	1 cut(s)	517
MhlI	GDGCHC	4 cut(s)	144, 363, 716, 1013
MluCI	AATT	7 cut(s)	117, 262, 510, 522, 537, 887, 1089
MmeI	TCCRAC	2 cut(s)	717, 1006
MseI	TTAA	4 cut(s)	213, 540, 821, 1308
MslI	CAYNNNNRTG	4 cut(s)	206, 426, 770, 906
MspA1I	CMGCKG	1 cut(s)	473
MspCI	CTTAAG	1 cut(s)	1307
MspI	CCGG	2 cut(s)	1021, 1048
MspR9I	CCNGG	6 cut(s)	92, 381, 612, 995, 1022, 1048
MunI	CAATTG	1 cut(s)	510
MvaI	CCWGG	4 cut(s)	92, 381, 612, 995
MvnI	CGCG	1 cut(s)	734
MwoI	GCNNNNNNNGC	3 cut(s)	294, 410, 467
NciI	CCSGG	2 cut(s)	1022, 1048
NdeII	GATC	4 cut(s)	54, 517, 784, 1319
NlaIII	CATG	8 cut(s)	116, 205, 331, 485, 728, 775, 832, 905
NlaIV	GGNNCC	1 cut(s)	370
NmuCI	GTSAC	3 cut(s)	484, 1075, 1129
NsbI	TGCGCA	1 cut(s)	508
NspI	RCATGY	2 cut(s)	832, 905
PaqCI	CACCTGC	1 cut(s)	1198
PceI	AGGCCT	1 cut(s)	379
PciI	ACATGT	1 cut(s)	901
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 69
PfeI	GAWTC	3 cut(s)	317, 494, 1172
PflFI	GACNNNGTC	2 cut(s)	101, 1022
PkrI	GCNGC	6 cut(s)	469, 472, 506, 589, 746, 967
PscI	ACATGT	1 cut(s)	901
Psp124BI	GAGCTC	1 cut(s)	363
Psp6I	CCWGG	4 cut(s)	90, 379, 610, 993
PspGI	CCWGG	4 cut(s)	90, 379, 610, 993
PspN4I	GGNNCC	1 cut(s)	370
PspPI	GGNCC	1 cut(s)	991
PsrI	GAACNNNNNNTAC	3 cut(s)	37, 933, 965
PstI	CTGCAG	2 cut(s)	472, 1209
PstNI	CAGNNNCTG	1 cut(s)	932
PsuI	RGATCY	1 cut(s)	517
PsyI	GACNNNGTC	2 cut(s)	101, 1022
PvuII	CAGCTG	1 cut(s)	473
RsaI	GTAC	5 cut(s)	14, 303, 347, 370, 951
RsaNI	GTAC	5 cut(s)	13, 302, 346, 369, 950
RseI	CAYNNNNRTG	4 cut(s)	206, 426, 770, 906
SacI	GAGCTC	1 cut(s)	363
SaqAI	TTAA	4 cut(s)	213, 540, 821, 1308
SatI	GCNGC	6 cut(s)	468, 471, 505, 588, 745, 966
Sau3AI	GATC	4 cut(s)	54, 517, 784, 1319
Sau96I	GGNCC	1 cut(s)	991
ScrFI	CCNGG	6 cut(s)	92, 381, 612, 995, 1022, 1048
SduI	GDGCHC	4 cut(s)	144, 363, 716, 1013
SfaNI	GCATC	4 cut(s)	67, 321, 649, 992
SfcI	CTRYAG	3 cut(s)	468, 474, 1205
SmiMI	CAYNNNNRTG	4 cut(s)	206, 426, 770, 906
SmlI	CTYRAG	3 cut(s)	1222, 1245, 1307
SmoI	CTYRAG	3 cut(s)	1222, 1245, 1307
Sse9I	AATT	7 cut(s)	117, 262, 510, 522, 537, 887, 1089
SseBI	AGGCCT	1 cut(s)	379
SsiI	CCGC	3 cut(s)	404, 732, 979
SspMI	CTAG	1 cut(s)	462
SstI	GAGCTC	1 cut(s)	363
StuI	AGGCCT	1 cut(s)	379
StyD4I	CCNGG	6 cut(s)	90, 379, 610, 993, 1020, 1046
TaaI	ACNGT	9 cut(s)	85, 101, 227, 425, 575, 763, 954, 1008, 1067
TaiI	ACGT	3 cut(s)	153, 946, 1140
TaqI	TCGA	6 cut(s)	57, 787, 898, 1170, 1291, 1318
TasI	AATT	7 cut(s)	117, 262, 510, 522, 537, 887, 1089
TatI	WGTACW	2 cut(s)	345, 949
TfiI	GAWTC	3 cut(s)	317, 494, 1172
Tru1I	TTAA	4 cut(s)	213, 540, 821, 1308
Tru9I	TTAA	4 cut(s)	213, 540, 821, 1308
TscAI	CASTG	7 cut(s)	106, 370, 428, 505, 580, 766, 959
TseFI	GTSAC	3 cut(s)	484, 1075, 1129
TseI	GCWGC	6 cut(s)	467, 470, 504, 587, 744, 965
Tsp45I	GTSAC	3 cut(s)	484, 1075, 1129
TspDTI	ATGAA	4 cut(s)	50, 190, 470, 684
TspGWI	ACGGA	4 cut(s)	310, 864, 887, 1148
TspRI	CASTG	7 cut(s)	106, 370, 428, 505, 580, 766, 959
Tth111I	GACNNNGTC	2 cut(s)	101, 1022
Vha464I	CTTAAG	1 cut(s)	1307
VneI	GTGCAC	1 cut(s)	1009
XagI	CCTNNNNNAGG	1 cut(s)	1245
XapI	RAATTY	3 cut(s)	117, 887, 1089
XceI	RCATGY	2 cut(s)	832, 905
XmiI	GTMKAC	1 cut(s)	1155
XspI	CTAG	1 cut(s)	462

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.