MD09G1020000.v1.1

Mitochondrial inner membrane protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009373
Physical Location & Seq
Forward (+)
1211406 .. 1213692
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1020000.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGAGGCTGAACCCGCACCGGAACCCGGATCCACAAGCTTCCCCTCCGCCGTCAACGGCGGCAAGACGTTGGAGGAATGTCAAACCATGATCCAGAGGAGCCTCCGGACTCCGATGGTGAAATTTCTGTTGAAGCATTTGGAGCAGTCTGGGTGTGGGATTGGGGACAGGTTCATCAAGGCCATTAATTGCAACAAGCAGATTGCCGGCGGTTATATTCGCGGCGAAGGGATACTGGTGTGCAGTAATCATATGACGATGCAAGATGATGTCAACCAAGTAGTGATACACGAGCTAATTCACGCTTTTGATGATTGTCGTGCTAAAAACTTGGAATGGTCTAATTGTGCTCATCATGCTTGCAGTGAGATTCGTGCTGGCCATCTAAGTGGTGACTGCCACTATAAAAGGGAATTGTTGCGGGGTTTTGTAAAGATTCGAGGTCATGAACAAGATTGTGTGAGACGAAGAGTTATGAAATCAGTCATTTCTAATCCATATTGCACAGAAGCAGCTGCAAAGGACGCCATGGAAGCTGTTTGGGATGTATGTTACAATGATACCCAGCCCTTTGACAGAGCTCCTTAA

Protein Analysis

196

Amino Acids

21.82

Weight (kDa)

6.49

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M76 PF09768 25 - 192 2.9e-61 Peptidase M76 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 222
AccIII TCCGGA 1 cut(s) 105
AciI CCGC 6 cut(s) 15, 48, 60, 210, 222, 421
AclWI GGATC 3 cut(s) 24, 37, 85
AcoI YGGCCR 1 cut(s) 379
AcsI RAATTY 1 cut(s) 122
AcyI GRCGYC 1 cut(s) 525
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 1 cut(s) 133
AluBI AGCT 5 cut(s) 39, 295, 515, 536, 581
AluI AGCT 5 cut(s) 39, 295, 515, 536, 581
Alw21I GWGCWC 2 cut(s) 352, 583
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 3 cut(s) 24, 37, 85
Aor13HI TCCGGA 1 cut(s) 105
AoxI GGCC 2 cut(s) 180, 379
ApeKI GCWGC 2 cut(s) 512, 515
ApoI RAATTY 1 cut(s) 122
AseI ATTAAT 1 cut(s) 186
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 130, 404
BaeI ACNNNNGTAYC 2 cut(s) 224, 257
BalI TGGCCA 1 cut(s) 381
BamHI GGATCC 1 cut(s) 29
BanII GRGCYC 1 cut(s) 583
BauI CACGAG 1 cut(s) 290
Bbv12I GWGCWC 2 cut(s) 352, 583
BbvI GCAGC 2 cut(s) 502, 524
BccI CCATC 2 cut(s) 109, 390
BceAI ACGGC 2 cut(s) 35, 73
BciVI GTATCC 1 cut(s) 225
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 457
BfuI GTATCC 1 cut(s) 225
BisI GCNGC 4 cut(s) 61, 223, 513, 516
BlsI GCNGC 4 cut(s) 62, 224, 514, 517
Bme1390I CCNGG 1 cut(s) 27
BmiI GGNNCC 3 cut(s) 24, 31, 101
BmrFI CCNGG 1 cut(s) 27
BmsI GCATC 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 27
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 1 cut(s) 528
BsaWI WCCGGW 2 cut(s) 19, 105
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 240
BseAI TCCGGA 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 528
BseGI GGATG 1 cut(s) 550
BseLI CCNNNNNNNGG 1 cut(s) 26
BseNI ACTGG 1 cut(s) 240
BseRI GAGGAG 1 cut(s) 112
BseXI GCAGC 2 cut(s) 502, 524
BseYI CCCAGC 1 cut(s) 564
BsgI GTGCAG 1 cut(s) 262
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 2 cut(s) 182, 381
BsiHKAI GWGCWC 2 cut(s) 352, 583
BsiSI CCGG 4 cut(s) 20, 27, 106, 207
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 1 cut(s) 457
BsmBI CGTCTC 1 cut(s) 457
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 2 cut(s) 182, 381
Bsp1286I GDGCHC 2 cut(s) 352, 583
Bsp13I TCCGGA 1 cut(s) 105
Bsp143I GATC 2 cut(s) 29, 90
Bsp19I CCATGG 1 cut(s) 528
BspACI CCGC 6 cut(s) 15, 48, 60, 210, 222, 421
BspANI GGCC 2 cut(s) 182, 381
BspEI TCCGGA 1 cut(s) 105
BspFNI CGCG 1 cut(s) 222
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 3 cut(s) 24, 31, 101
BspPI GGATC 3 cut(s) 24, 37, 85
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 1 cut(s) 240
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 528
BssMI GATC 2 cut(s) 29, 90
BssNI GRCGYC 1 cut(s) 525
BssSI CACGAG 1 cut(s) 290
BssT1I CCWWGG 1 cut(s) 528
Bst2BI CACGAG 1 cut(s) 290
Bst6I CTCTTC 1 cut(s) 463
BstACI GRCGYC 1 cut(s) 525
BstC8I GCNNGC 3 cut(s) 208, 361, 379
BstDEI CTNAG 1 cut(s) 386
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 1 cut(s) 550
BstFNI CGCG 1 cut(s) 222
BstKTI GATC 2 cut(s) 32, 93
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 2 cut(s) 29, 90
BstMWI GCNNNNNNNGC 5 cut(s) 14, 142, 356, 524, 533
BstSCI CCNGG 1 cut(s) 25
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 2 cut(s) 502, 524
BstX2I RGATCY 1 cut(s) 29
BstXI CCANNNNNNTGG 1 cut(s) 389
BstYI RGATCY 1 cut(s) 29
BsuI GTATCC 1 cut(s) 225
BsuRI GGCC 2 cut(s) 182, 381
BtgI CCRYGG 1 cut(s) 528
BtsCI GGATG 1 cut(s) 550
BtsI GCAGTG 1 cut(s) 370
BtsIMutI CAGTG 1 cut(s) 370
Cac8I GCNNGC 3 cut(s) 208, 361, 379
CciI TCATGA 1 cut(s) 445
Cfr10I RCCGGY 1 cut(s) 206
CseI GACGC 1 cut(s) 533
CviAII CATG 4 cut(s) 88, 356, 446, 529
DdeI CTNAG 1 cut(s) 386
DpnI GATC 2 cut(s) 31, 92
DpnII GATC 2 cut(s) 29, 90
EaeI YGGCCR 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 463
EarI CTCTTC 1 cut(s) 463
EciI GGCGGA 1 cut(s) 37
Ecl136II GAGCTC 1 cut(s) 581
Eco130I CCWWGG 1 cut(s) 528
Eco24I GRGCYC 1 cut(s) 583
Eco53kI GAGCTC 1 cut(s) 581
EcoICRI GAGCTC 1 cut(s) 581
EcoT14I CCWWGG 1 cut(s) 528
EcoT38I GRGCYC 1 cut(s) 583
ErhI CCWWGG 1 cut(s) 528
Esp3I CGTCTC 1 cut(s) 457
FaeI CATG 4 cut(s) 91, 359, 449, 532
FaqI GGGAC 1 cut(s) 179
FatI CATG 4 cut(s) 87, 355, 445, 528
FauI CCCGC 2 cut(s) 22, 414
FauNDI CATATG 1 cut(s) 252
Fnu4HI GCNGC 4 cut(s) 61, 223, 513, 516
FokI GGATG 1 cut(s) 557
FriOI GRGCYC 1 cut(s) 583
Fsp4HI GCNGC 4 cut(s) 61, 223, 513, 516
GluI GCNGC 4 cut(s) 61, 223, 513, 516
GsaI CCCAGC 1 cut(s) 568
HaeIII GGCC 2 cut(s) 182, 381
HapII CCGG 4 cut(s) 20, 27, 106, 207
HgaI GACGC 1 cut(s) 533
Hin1I GRCGYC 1 cut(s) 525
Hin1II CATG 4 cut(s) 91, 359, 449, 532
HincII GTYRAC 2 cut(s) 55, 274
HindII GTYRAC 2 cut(s) 55, 274
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 3 cut(s) 109, 370, 436
HpaII CCGG 4 cut(s) 20, 27, 106, 207
HphI GGTGA 2 cut(s) 130, 404
Hpy166II GTNNAC 2 cut(s) 55, 274
Hpy188I TCNGA 1 cut(s) 114
Hpy188III TCNNGA 3 cut(s) 94, 106, 446
Hpy8I GTNNAC 2 cut(s) 55, 274
HpyAV CCTTC 1 cut(s) 221
HpyCH4IV ACGT 1 cut(s) 68
HpyCH4V TGCA 6 cut(s) 192, 243, 262, 363, 504, 518
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 142, 356, 524, 533
HpyF3I CTNAG 1 cut(s) 386
HpySE526I ACGT 1 cut(s) 68
Hsp92I GRCGYC 1 cut(s) 525
Hsp92II CATG 4 cut(s) 91, 359, 449, 532
Kpn2I TCCGGA 1 cut(s) 105
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
Kzo9I GATC 2 cut(s) 29, 90
LmnI GCTCC 3 cut(s) 99, 142, 586
Lsp1109I GCAGC 2 cut(s) 502, 524
LweI GCATC 1 cut(s) 249
MaeII ACGT 1 cut(s) 68
MaeIII GTNAC 2 cut(s) 392, 551
MalI GATC 2 cut(s) 31, 92
MboI GATC 2 cut(s) 29, 90
MboII GAAGA 1 cut(s) 480
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 2 cut(s) 352, 583
MlsI TGGCCA 1 cut(s) 381
MluCI AATT 5 cut(s) 122, 187, 297, 343, 413
MluNI TGGCCA 1 cut(s) 381
MlyI GAGTC 1 cut(s) 103
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 5 cut(s) 55, 67, 90, 113, 434
Mox20I TGGCCA 1 cut(s) 381
MroI TCCGGA 1 cut(s) 105
MroNI GCCGGC 1 cut(s) 206
MscI TGGCCA 1 cut(s) 381
MseI TTAA 2 cut(s) 186, 586
MslI CAYNNNNRTG 1 cut(s) 387
Msp20I TGGCCA 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 515
MspI CCGG 4 cut(s) 20, 27, 106, 207
MspR9I CCNGG 1 cut(s) 27
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 5 cut(s) 14, 142, 356, 524, 533
NaeI GCCGGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 27
NcoI CCATGG 1 cut(s) 528
NdeI CATATG 1 cut(s) 252
NdeII GATC 2 cut(s) 29, 90
NgoMIV GCCGGC 1 cut(s) 206
NlaIII CATG 4 cut(s) 91, 359, 449, 532
NlaIV GGNNCC 3 cut(s) 24, 31, 101
NmuCI GTSAC 1 cut(s) 392
PagI TCATGA 1 cut(s) 445
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 2 cut(s) 370, 436
PkrI GCNGC 4 cut(s) 62, 224, 514, 517
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PshBI ATTAAT 1 cut(s) 186
Psp124BI GAGCTC 1 cut(s) 583
PspFI CCCAGC 1 cut(s) 564
PspN4I GGNNCC 3 cut(s) 24, 31, 101
PsuI RGATCY 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 515
RseI CAYNNNNRTG 1 cut(s) 387
SacI GAGCTC 1 cut(s) 583
SaqAI TTAA 2 cut(s) 186, 586
SatI GCNGC 4 cut(s) 61, 223, 513, 516
Sau3AI GATC 2 cut(s) 29, 90
SchI GAGTC 1 cut(s) 103
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 2 cut(s) 352, 583
SetI ASST 8 cut(s) 41, 71, 173, 297, 445, 517, 538, 583
SfaNI GCATC 1 cut(s) 249
SmiMI CAYNNNNRTG 1 cut(s) 387
Sse9I AATT 5 cut(s) 122, 187, 297, 343, 413
SsiI CCGC 6 cut(s) 15, 48, 60, 210, 222, 421
SstI GAGCTC 1 cut(s) 583
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 1 cut(s) 528
TaiI ACGT 1 cut(s) 71
TaqI TCGA 1 cut(s) 439
TasI AATT 5 cut(s) 122, 187, 297, 343, 413
TauI GCSGC 2 cut(s) 63, 225
TfiI GAWTC 2 cut(s) 370, 436
Tru1I TTAA 2 cut(s) 186, 586
Tru9I TTAA 2 cut(s) 186, 586
TscAI CASTG 1 cut(s) 370
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 2 cut(s) 512, 515
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 3 cut(s) 163, 462, 491
TspRI CASTG 1 cut(s) 370
VspI ATTAAT 1 cut(s) 186
XapI RAATTY 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.