Rw2G053410

Mitochondrial inner membrane protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009373
Physical Location & Seq
Reverse (-)
81168536 .. 81170395
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G053410.1

Sequence Viewer

Length: 600 bp
ATGGCAGACGAACCCACCCCAGAACCCGGAACCAGCACCTCCTCCTCCTCCTCCTCCTCCGCCGTCAACGGCGGAAAGACCGTCAACGAATGCCAGGACATGATTCGGAGAAGCCTCCGAACTCCGATGGTGAAGTTTCTGCTGGAGCATCTGGAGAAATCTGGGTGTGGGATTGGGGACGGGTTCATCAAGGCCGTTCATTGCGAGAAGAAGATTGCCGGAGGATACACCCGCGGCGGAGGGATAATGGTGTGTAGTAATCACATGAACATGCAAGATGAGGTCAACCAAGTAGTGATACATGAGCTAATTCATGCTTTTGATGATTGTCGGGCTGCAAACTTGAACTGGGCTAATTGCGCTCATCATGCTTGTAGCGAGATTCGTGCTGGCCATCTTAGTGGTGATTGCCACTATAAACGTGAATTTTTGCGGGGTTTTATGAAGATTCGAGGCCATGAACAAGATTGCGTGAGACGAAGAGTTATGAAATCGGTCACTGCTAATCCCTACTGCTCAGAAGCAGCAGCAAAGGATGCCATGGAAGCTGTGTGGGATGTTTGTTACAATGATACGCAGCCCTTTGACAGAGCCCCTTGA

Protein Analysis

199

Amino Acids

21.97

Weight (kDa)

6.54

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M76 PF09768 30 - 196 3.8e-61 Peptidase M76 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 234
AciI CCGC 6 cut(s) 60, 72, 232, 234, 237, 433
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 1 cut(s) 425
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 1 cut(s) 346
AjnI CCWGG 1 cut(s) 93
AluBI AGCT 2 cut(s) 307, 548
AluI AGCT 2 cut(s) 307, 548
Alw26I GTCTC 1 cut(s) 469
AoxI GGCC 3 cut(s) 192, 391, 454
ApeKI GCWGC 4 cut(s) 335, 524, 527, 577
ApoI RAATTY 1 cut(s) 425
AspLEI GCGC 1 cut(s) 362
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 142, 416
BalI TGGCCA 1 cut(s) 393
BanII GRGCYC 1 cut(s) 595
BbvI GCAGC 4 cut(s) 322, 536, 539, 589
BccI CCATC 2 cut(s) 121, 402
BceAI ACGGC 3 cut(s) 47, 85, 179
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 1 cut(s) 95
BciVI GTATCC 1 cut(s) 218
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 469
BfuI GTATCC 1 cut(s) 218
BisI GCNGC 5 cut(s) 235, 336, 525, 528, 578
BlsI GCNGC 5 cut(s) 236, 337, 526, 529, 579
Bme1390I CCNGG 2 cut(s) 27, 95
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 2 cut(s) 27, 95
BmrI ACTGGG 1 cut(s) 358
BmsI GCATC 2 cut(s) 157, 526
BmuI ACTGGG 1 cut(s) 358
BpmI CTGGAG 2 cut(s) 164, 173
BpuMI CCSGG 1 cut(s) 27
BsaJI CCNNGG 2 cut(s) 232, 540
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 353
Bse3DI GCAATG 1 cut(s) 199
BseBI CCWGG 1 cut(s) 95
BseDI CCNNGG 2 cut(s) 232, 540
BseGI GGATG 2 cut(s) 541, 562
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 1 cut(s) 353
BseRI GAGGAG 6 cut(s) 31, 34, 37, 40, 43, 46
BseXI GCAGC 4 cut(s) 322, 536, 539, 589
Bsh1236I CGCG 1 cut(s) 234
BshFI GGCC 3 cut(s) 194, 393, 456
BsiSI CCGG 2 cut(s) 27, 219
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 1 cut(s) 469
BsmBI CGTCTC 1 cut(s) 469
BsmFI GGGAC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 3 cut(s) 194, 393, 456
Bsp1286I GDGCHC 1 cut(s) 595
Bsp19I CCATGG 1 cut(s) 540
BspACI CCGC 6 cut(s) 60, 72, 232, 234, 237, 433
BspANI GGCC 3 cut(s) 194, 393, 456
BspCNI CTCAG 1 cut(s) 530
BspFNI CGCG 1 cut(s) 234
BspLI GGNNCC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 199
BsrI ACTGG 1 cut(s) 353
BssECI CCNNGG 2 cut(s) 232, 540
BssT1I CCWWGG 1 cut(s) 540
Bst2UI CCWGG 1 cut(s) 95
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 1 cut(s) 475
BstAPI GCANNNNNTGC 1 cut(s) 536
BstC8I GCNNGC 1 cut(s) 391
BstDEI CTNAG 2 cut(s) 398, 517
BstDSI CCRYGG 2 cut(s) 232, 540
BstF5I GGATG 2 cut(s) 541, 562
BstFNI CGCG 1 cut(s) 234
BstHHI GCGC 1 cut(s) 362
BstMAI GTCTC 1 cut(s) 469
BstMWI GCNNNNNNNGC 4 cut(s) 359, 368, 536, 545
BstNI CCWGG 1 cut(s) 95
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 2 cut(s) 25, 93
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 4 cut(s) 322, 536, 539, 589
BstXI CCANNNNNNTGG 1 cut(s) 401
BsuI GTATCC 1 cut(s) 218
BsuRI GGCC 3 cut(s) 194, 393, 456
BtgI CCRYGG 2 cut(s) 232, 540
BtsCI GGATG 2 cut(s) 541, 562
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 1 cut(s) 498
Cac8I GCNNGC 1 cut(s) 391
CfoI GCGC 1 cut(s) 362
Cfr42I CCGCGG 1 cut(s) 235
CviAII CATG 8 cut(s) 100, 265, 271, 302, 314, 368, 458, 541
DdeI CTNAG 2 cut(s) 398, 517
EaeI YGGCCR 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 475
EarI CTCTTC 1 cut(s) 475
EciI GGCGGA 3 cut(s) 49, 87, 252
Eco130I CCWWGG 1 cut(s) 540
Eco24I GRGCYC 1 cut(s) 595
EcoRII CCWGG 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 540
EcoT38I GRGCYC 1 cut(s) 595
ErhI CCWWGG 1 cut(s) 540
Esp3I CGTCTC 1 cut(s) 469
FaeI CATG 8 cut(s) 103, 268, 274, 305, 317, 371, 461, 544
FaqI GGGAC 1 cut(s) 191
FatI CATG 8 cut(s) 99, 264, 270, 301, 313, 367, 457, 540
FauI CCCGC 2 cut(s) 239, 426
Fnu4HI GCNGC 5 cut(s) 235, 336, 525, 528, 578
FokI GGATG 2 cut(s) 548, 569
FriOI GRGCYC 1 cut(s) 595
Fsp4HI GCNGC 5 cut(s) 235, 336, 525, 528, 578
GlaI GCGC 1 cut(s) 361
GluI GCNGC 5 cut(s) 235, 336, 525, 528, 578
GsuI CTGGAG 2 cut(s) 164, 173
HaeIII GGCC 3 cut(s) 194, 393, 456
HapII CCGG 2 cut(s) 27, 219
HhaI GCGC 1 cut(s) 362
Hin1II CATG 8 cut(s) 103, 268, 274, 305, 317, 371, 461, 544
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HincII GTYRAC 3 cut(s) 67, 85, 286
HindII GTYRAC 3 cut(s) 67, 85, 286
HinfI GANTC 3 cut(s) 103, 382, 448
HpaII CCGG 2 cut(s) 27, 219
HphI GGTGA 2 cut(s) 142, 416
Hpy166II GTNNAC 3 cut(s) 67, 85, 286
Hpy188I TCNGA 4 cut(s) 108, 119, 126, 520
Hpy188III TCNNGA 1 cut(s) 152
Hpy8I GTNNAC 3 cut(s) 67, 85, 286
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 421
HpyCH4V TGCA 2 cut(s) 274, 338
HpyF10VI GCNNNNNNNGC 4 cut(s) 359, 368, 536, 545
HpyF3I CTNAG 2 cut(s) 398, 517
HpySE526I ACGT 1 cut(s) 421
Hsp92II CATG 8 cut(s) 103, 268, 274, 305, 317, 371, 461, 544
HspAI GCGC 1 cut(s) 360
KspI CCGCGG 1 cut(s) 235
LmnI GCTCC 1 cut(s) 145
Lsp1109I GCAGC 4 cut(s) 322, 536, 539, 589
LweI GCATC 2 cut(s) 157, 526
MaeII ACGT 1 cut(s) 421
MaeIII GTNAC 2 cut(s) 496, 563
MboII GAAGA 4 cut(s) 220, 223, 457, 492
MhlI GDGCHC 1 cut(s) 595
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 3 cut(s) 309, 355, 425
MluNI TGGCCA 1 cut(s) 393
Mox20I TGGCCA 1 cut(s) 393
MscI TGGCCA 1 cut(s) 393
MslI CAYNNNNRTG 2 cut(s) 269, 399
Msp20I TGGCCA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 234
MspI CCGG 2 cut(s) 27, 219
MspR9I CCNGG 2 cut(s) 27, 95
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 1 cut(s) 95
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 4 cut(s) 359, 368, 536, 545
NciI CCSGG 1 cut(s) 27
NcoI CCATGG 1 cut(s) 540
NlaIII CATG 8 cut(s) 103, 268, 274, 305, 317, 371, 461, 544
NlaIV GGNNCC 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 496
NspI RCATGY 1 cut(s) 274
PctI GAATGC 1 cut(s) 95
PfeI GAWTC 3 cut(s) 103, 382, 448
PkrI GCNGC 5 cut(s) 236, 337, 526, 529, 579
Psp6I CCWGG 1 cut(s) 93
PspGI CCWGG 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 31
RseI CAYNNNNRTG 2 cut(s) 269, 399
SacII CCGCGG 1 cut(s) 235
SatI GCNGC 5 cut(s) 235, 336, 525, 528, 578
ScrFI CCNGG 2 cut(s) 27, 95
SduI GDGCHC 1 cut(s) 595
SetI ASST 5 cut(s) 41, 285, 309, 424, 550
SfaNI GCATC 2 cut(s) 157, 526
Sfr303I CCGCGG 1 cut(s) 235
SgrBI CCGCGG 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 269, 399
Sse9I AATT 3 cut(s) 309, 355, 425
SsiI CCGC 6 cut(s) 60, 72, 232, 234, 237, 433
StyD4I CCNGG 2 cut(s) 25, 93
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 424
TaqI TCGA 1 cut(s) 451
TaqII GACCGA 1 cut(s) 484
TasI AATT 3 cut(s) 309, 355, 425
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 3 cut(s) 103, 382, 448
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 4 cut(s) 335, 524, 527, 577
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 7 cut(s) 175, 188, 281, 302, 458, 474, 503
TspRI CASTG 1 cut(s) 505
XapI RAATTY 1 cut(s) 425
XceI RCATGY 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.