Rroxscaffold_2G00078710

Mitochondrial inner membrane protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009373
Physical Location & Seq
Forward (+)
1946581 .. 1948600
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00078710.1

Sequence Viewer

Length: 591 bp
ATGGCAGACGAACCCACCCCAGAACCCGGAACCAGCACCTCCTCCTCCTCCGCCGTCAACGGCGGCAAGACCGTCAACGAATGCCAAGACATGATTCGGAGAAGCCTCCGAACTCCGATGGTGAAGTTTCTGCTGGAGCATCTGGAGAAATCTGGGTGTGGGATTGGGGACGGGTTCATCAAGGCCGTTCATTGCGAGAAGAAGATTGCCGGAGGATACACTCGTGGCGGAGGGATAATGGTGTGTAGTAACCACATGAACATGCAAGATGAGGTCAACCAAGTAGTGATACATGAGCTCATTCATGCGTTTGATGATTGTCGGGCTGCAAACTTGAACTGGGCTAATTGCGCTCATCATGCTTGTAGCGAGATTCGTGCTGGCCATCTTAGTGGTGATTGCCACTATAAACGTGAATTTTTGCGGGGTTTTATGAAGATTCGAGGCCATGAACAAGATTGCGTGAGACGAAGAGTTATGAAATCGGTCACTGCTAATCCCTACTGCTCAGAAGCAGCAGCAAAGGATGCCATGGAAGCTGTGTGGGATGTTTGTTACAATGATACGCAGCCCTTTGACAGAGCCCCTTGA

Protein Analysis

196

Amino Acids

21.7

Weight (kDa)

6.54

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M76 PF09768 27 - 193 3.6e-61 Peptidase M76 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 51, 63, 228, 424
AcoI YGGCCR 1 cut(s) 382
AcsI RAATTY 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 2 cut(s) 298, 539
AluI AGCT 2 cut(s) 298, 539
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 1 cut(s) 460
AoxI GGCC 3 cut(s) 183, 382, 445
ApeKI GCWGC 4 cut(s) 326, 515, 518, 568
ApoI RAATTY 1 cut(s) 416
AspLEI GCGC 1 cut(s) 353
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 133, 407
BaeI ACNNNNGTAYC 2 cut(s) 208, 241
BalI TGGCCA 1 cut(s) 384
BanII GRGCYC 2 cut(s) 300, 586
BauI CACGAG 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 4 cut(s) 313, 527, 530, 580
BccI CCATC 2 cut(s) 112, 393
BceAI ACGGC 3 cut(s) 38, 76, 170
BcgI CGANNNNNNTGC 2 cut(s) 359, 393
BciVI GTATCC 1 cut(s) 209
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 460
BfuI GTATCC 1 cut(s) 209
BisI GCNGC 5 cut(s) 64, 327, 516, 519, 569
BlsI GCNGC 5 cut(s) 65, 328, 517, 520, 570
Bme1390I CCNGG 1 cut(s) 27
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 27
BmrI ACTGGG 1 cut(s) 349
BmsI GCATC 2 cut(s) 148, 517
BmuI ACTGGG 1 cut(s) 349
BpmI CTGGAG 2 cut(s) 155, 164
BpuMI CCSGG 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 531
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 344
Bse3DI GCAATG 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 531
BseGI GGATG 2 cut(s) 532, 553
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 1 cut(s) 344
BseRI GAGGAG 3 cut(s) 31, 34, 37
BseXI GCAGC 4 cut(s) 313, 527, 530, 580
BshFI GGCC 3 cut(s) 185, 384, 447
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 2 cut(s) 27, 210
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 1 cut(s) 460
BsmBI CGTCTC 1 cut(s) 460
BsmFI GGGAC 1 cut(s) 182
BsmI GAATGC 1 cut(s) 86
BsnI GGCC 3 cut(s) 185, 384, 447
Bsp1286I GDGCHC 2 cut(s) 300, 586
Bsp19I CCATGG 1 cut(s) 531
BspACI CCGC 4 cut(s) 51, 63, 228, 424
BspANI GGCC 3 cut(s) 185, 384, 447
BspCNI CTCAG 1 cut(s) 521
BspLI GGNNCC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 190
BsrI ACTGG 1 cut(s) 344
BssECI CCNNGG 1 cut(s) 531
BssSI CACGAG 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 531
Bst2BI CACGAG 1 cut(s) 222
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 1 cut(s) 466
BstAPI GCANNNNNTGC 1 cut(s) 527
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 2 cut(s) 389, 508
BstDSI CCRYGG 1 cut(s) 531
BstF5I GGATG 2 cut(s) 532, 553
BstHHI GCGC 1 cut(s) 353
BstMAI GTCTC 1 cut(s) 460
BstMWI GCNNNNNNNGC 4 cut(s) 350, 359, 527, 536
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 1 cut(s) 25
BstV1I GCAGC 4 cut(s) 313, 527, 530, 580
BstXI CCANNNNNNTGG 1 cut(s) 392
BsuI GTATCC 1 cut(s) 209
BsuRI GGCC 3 cut(s) 185, 384, 447
BtgI CCRYGG 1 cut(s) 531
BtsCI GGATG 2 cut(s) 532, 553
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 1 cut(s) 489
Cac8I GCNNGC 1 cut(s) 382
CfoI GCGC 1 cut(s) 353
CviAII CATG 8 cut(s) 91, 256, 262, 293, 305, 359, 449, 532
DdeI CTNAG 2 cut(s) 389, 508
EaeI YGGCCR 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 466
EarI CTCTTC 1 cut(s) 466
EciI GGCGGA 2 cut(s) 40, 243
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 531
Eco24I GRGCYC 2 cut(s) 300, 586
Eco53kI GAGCTC 1 cut(s) 298
EcoICRI GAGCTC 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 531
EcoT38I GRGCYC 2 cut(s) 300, 586
ErhI CCWWGG 1 cut(s) 531
Esp3I CGTCTC 1 cut(s) 460
FaeI CATG 8 cut(s) 94, 259, 265, 296, 308, 362, 452, 535
FaqI GGGAC 1 cut(s) 182
FatI CATG 8 cut(s) 90, 255, 261, 292, 304, 358, 448, 531
FauI CCCGC 1 cut(s) 417
Fnu4HI GCNGC 5 cut(s) 64, 327, 516, 519, 569
FokI GGATG 2 cut(s) 539, 560
FriOI GRGCYC 2 cut(s) 300, 586
Fsp4HI GCNGC 5 cut(s) 64, 327, 516, 519, 569
GlaI GCGC 1 cut(s) 352
GluI GCNGC 5 cut(s) 64, 327, 516, 519, 569
GsuI CTGGAG 2 cut(s) 155, 164
HaeIII GGCC 3 cut(s) 185, 384, 447
HapII CCGG 2 cut(s) 27, 210
HhaI GCGC 1 cut(s) 353
Hin1II CATG 8 cut(s) 94, 259, 265, 296, 308, 362, 452, 535
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HincII GTYRAC 3 cut(s) 58, 76, 277
HindII GTYRAC 3 cut(s) 58, 76, 277
HinfI GANTC 3 cut(s) 94, 373, 439
HpaII CCGG 2 cut(s) 27, 210
HphI GGTGA 2 cut(s) 133, 407
Hpy166II GTNNAC 3 cut(s) 58, 76, 277
Hpy188I TCNGA 4 cut(s) 99, 110, 117, 511
Hpy188III TCNNGA 1 cut(s) 143
Hpy8I GTNNAC 3 cut(s) 58, 76, 277
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 1 cut(s) 412
HpyCH4V TGCA 2 cut(s) 265, 329
HpyF10VI GCNNNNNNNGC 4 cut(s) 350, 359, 527, 536
HpyF3I CTNAG 2 cut(s) 389, 508
HpySE526I ACGT 1 cut(s) 412
Hsp92II CATG 8 cut(s) 94, 259, 265, 296, 308, 362, 452, 535
HspAI GCGC 1 cut(s) 351
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 9 cut(s) 33, 40, 46, 119, 128, 138, 223, 325, 366
Lsp1109I GCAGC 4 cut(s) 313, 527, 530, 580
LweI GCATC 2 cut(s) 148, 517
MaeII ACGT 1 cut(s) 412
MaeIII GTNAC 3 cut(s) 248, 487, 554
MboII GAAGA 4 cut(s) 211, 214, 448, 483
MhlI GDGCHC 2 cut(s) 300, 586
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 2 cut(s) 346, 416
MluNI TGGCCA 1 cut(s) 384
MnlI CCTC 9 cut(s) 49, 52, 55, 58, 116, 206, 224, 265, 437
Mox20I TGGCCA 1 cut(s) 384
MscI TGGCCA 1 cut(s) 384
MslI CAYNNNNRTG 2 cut(s) 260, 390
Msp20I TGGCCA 1 cut(s) 384
MspI CCGG 2 cut(s) 27, 210
MspR9I CCNGG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 86
MwoI GCNNNNNNNGC 4 cut(s) 350, 359, 527, 536
NciI CCSGG 1 cut(s) 27
NcoI CCATGG 1 cut(s) 531
NlaIII CATG 8 cut(s) 94, 259, 265, 296, 308, 362, 452, 535
NlaIV GGNNCC 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 487
NspI RCATGY 1 cut(s) 265
PctI GAATGC 1 cut(s) 86
PfeI GAWTC 3 cut(s) 94, 373, 439
PkrI GCNGC 5 cut(s) 65, 328, 517, 520, 570
Psp124BI GAGCTC 1 cut(s) 300
PspN4I GGNNCC 1 cut(s) 31
RseI CAYNNNNRTG 2 cut(s) 260, 390
SacI GAGCTC 1 cut(s) 300
SatI GCNGC 5 cut(s) 64, 327, 516, 519, 569
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 2 cut(s) 300, 586
SetI ASST 5 cut(s) 41, 276, 300, 415, 541
SfaNI GCATC 2 cut(s) 148, 517
SmiMI CAYNNNNRTG 2 cut(s) 260, 390
Sse9I AATT 2 cut(s) 346, 416
SsiI CCGC 4 cut(s) 51, 63, 228, 424
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 1 cut(s) 415
TaqI TCGA 1 cut(s) 442
TaqII GACCGA 1 cut(s) 475
TasI AATT 2 cut(s) 346, 416
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 3 cut(s) 94, 373, 439
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 1 cut(s) 487
TseI GCWGC 4 cut(s) 326, 515, 518, 568
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 7 cut(s) 166, 179, 272, 293, 449, 465, 494
TspRI CASTG 1 cut(s) 496
XapI RAATTY 1 cut(s) 416
XceI RCATGY 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.