MD09G1039200.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012888
Physical Location & Seq
Reverse (-)
2393632 .. 2395323
1692 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1039200.v1.1.491

Sequence Viewer

Length: 1692 bp
ATGGAGTTTCAGGAAGCTATGTTGAAGCTGATGGTCCATAAATCAAAAGAGAACGAATGGGCTTTCGCCACGCCGGAAAGAGCGGCGGAGATGAGCAGTGCAAGCCCAAGTCCCCGGCGGAAATGGAAGCTTTTTCAGAGGTCGTCGTCGGCAATTCCGTCGAAAACTTCAAAGCCGCAAGCTCCCAAAGAGTTCCTCTGTCCAATCTCCGGCTCTCTGATGGCGGACCCGGTCATCGTCTCCTCCGGCCACACCTTCGAGCGCGCCTGCATCCAGGCCTGCAAGTCCCTCAGCTTCACTTCCGCTCTCCCGGACTCTCCGCCGCCCGATTTCTCCTCCGTAATCTCCAACCTCGCTCTCAAATCTGCCATTCTCAGTTGGTGCCGGAAGTCCTCGGTCGATCCTCCCAAGCCCCTCGATGTCGCCTCCGCCGAGAAGCTCGTCCGCGCCTCCATTGCTTCCCGGAACGAAACCAACCCCCAAAACGTCGTAGTTTCGGAGAATCAGTTGCTGATCAACGGTGTTAAGGATACCCCTACTGTGAATTTCATTCACGCGGCCACCGACCTGACGCGGCGCTCGAGTCACTTCCCCTCAAGCTCCGATGAATCGGTCACCGCGGCTCCGCCTCTCCCGTTCTCAACTCAGCCGAGTTGCTACTCATCTCCGTCGTCTTCCTTCTCCGAAAACGAAATCCTAACCGTTAACGGCAACAGTAATCCGTCCCGCTCTGTAGAAGAAGACGAAATCTCGATCAAGCTCAGAAGCTGCCACGTGTTCGAAATCGAGGAGGCCCTGGCTTCGCTGAGGAAGATCACGCGGACCAGAGAGGACGCCCGGGCCCGACTCTGCACCCCGCGGTTGCTCTCCGGCCTCCGCCCTCTGATTATATCCAGGTACACAGCCGTTCAGGTGGACTCCGTCGCAGCGTTGGTCAATCTATCGCTGGAAAAGTCAAACAAGATTAAGATCGTACGGTCAGGAGTCCTCCCTCCTCTGATCGACGTGCTCAAGGCAGGATCCCGTGAGGCGCAGGAACACGCTTCCGGTGCGTTCTTCAGCTTGGCACTTGATGACGACTGCAAGACCGCGATTGGCGCATTGGGCGCTTTGCCGCCTTTGCTGCACTTACTCCGGTCCGAGAGCGAGCGGACTCGGCATGACTCGGCACTCGCATTGTACCACCTCTCACTAGTTCAGAGCAACCGGTCCAAGTTGGTTAAGCTCGGGTCGGTTCCGGTCCTGTTGAGAATGGTAAAGTCTGGCCACATGACGGGTCGGGTATTGCTCATATTGTGTAATTTGAGTTTGTGTGCGGACGGGCGGGCCGCATTGTTGGATTCGGGTGGGGTGGAGTGTTTGGTGAGTGTGTTAAGGGGAAACGAGTTTGACTCGAAGGCGACTCAAGAGAGTTGTGTGGCCACATTGTATGGGCTGAGTTACGGCGGTTTGAGGTTCAAAGGGTTGGCGAAGACGGCGGGCGTGGTGGGGGTGTTGAGAGAGGTGGAGAAAAAGGGAAGCGAACGGGCGAGGGAGAAAGCGAGGAGAATGTTGGAGATGATGAGAGGGAAAGAGAAGGAAGAAGAAGAAGTGGATTGGGAGGAATTGCTCGACTCGGGGTTTGGGAGTCGAACTCGGTGTCAAACCGGTGGTGGGTTAGGCGGGTTGAGTGCCAACTCATCTGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

564

Amino Acids

61.03

Weight (kDa)

8.78

Isoelectric Point (pI)

53.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 61 - 130 2.1e-11 U-box domain
ARM_PUB PF25598 263 - 532 6.3e-09 Plant U-box protein ARM repeat region
Arm PF00514 358 - 397 7.2e-07 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 359 - 491 9e-08 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40140
fragaria_vesca FvH4_6g50090
malus_domestica MD09G1039200.v1.1 MD17G1040200.v1.1
prunus_persica Prupe.3G278800_v2.0.a1
pyrus_communis pycom111g03090 pycom17g03600
rosa_chinensis RchiOBHm_Chr2g0170361
rosa_laevigata RLG00000021949
rosa_multiflora Rmu_co8477427.1_g000001 Rmu_sc0011601.1_g000012
rosa_roxburghii Rroxscaffold_2G00081400
rosa_rugosa Rorug02G0548500
rosa_samantha Rh2AG619900 Rh2BG630900 Rh2CG600900 Rh2DG643800
rosa_wichuraiana Rw2G051360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 381
AccBSI CCGCTC 4 cut(s) 83, 305, 729, 1150
AccII CGCG 8 cut(s) 264, 447, 557, 574, 620, 820, 859, 1091
AclWI GGATC 3 cut(s) 395, 1014, 1027
AcoI YGGCCR 4 cut(s) 247, 558, 1264, 1419
AcsI RAATTY 2 cut(s) 544, 1685
AcuI CTGAAG 1 cut(s) 1042
AcvI CACGTG 1 cut(s) 775
AcyI GRCGYC 1 cut(s) 834
AfaI GTAC 3 cut(s) 899, 975, 1181
AfiI CCNNNNNNNGG 3 cut(s) 209, 1273, 1653
AflIII ACRYGT 1 cut(s) 774
AgeI ACCGGT 2 cut(s) 1206, 1646
AgsI TTSAA 3 cut(s) 25, 171, 1459
AhlI ACTAGT 1 cut(s) 1192
AjiI CACGTC 1 cut(s) 1006
AjnI CCWGG 3 cut(s) 273, 795, 893
Alw21I GWGCWC 1 cut(s) 1011
Alw26I GTCTC 1 cut(s) 244
AlwI GGATC 3 cut(s) 395, 1014, 1027
AlwNI CAGNNNCTG 2 cut(s) 511, 768
Ama87I CYCGRG 4 cut(s) 580, 837, 1226, 1615
AoxI GGCC 9 cut(s) 247, 276, 558, 792, 840, 871, 1264, 1326, 1419
ApaI GGGCCC 1 cut(s) 844
ApeKI GCWGC 3 cut(s) 768, 926, 1123
ApoI RAATTY 2 cut(s) 544, 1685
ArsI GACNNNNNNTTYG 4 cut(s) 1604, 1624, 1636, 1656
AsiGI ACCGGT 2 cut(s) 1206, 1646
AspLEI GCGC 7 cut(s) 264, 266, 449, 579, 1033, 1100, 1109
AsuC2I CCSGG 6 cut(s) 115, 230, 311, 463, 838, 839
AsuHPI GGTGA 2 cut(s) 607, 1375
AsuII TTCGAA 1 cut(s) 780
AvaI CYCGRG 4 cut(s) 580, 837, 1226, 1615
AvaII GGWCC 6 cut(s) 34, 226, 822, 1137, 1209, 1240
BaeGI GKGCMC 1 cut(s) 844
BalI TGGCCA 2 cut(s) 1266, 1421
BamHI GGATCC 1 cut(s) 1019
BanI GGYRCC 1 cut(s) 381
BanII GRGCYC 1 cut(s) 844
BbrPI CACGTG 1 cut(s) 775
BbsI GAAGAC 3 cut(s) 666, 747, 1478
Bbv12I GWGCWC 1 cut(s) 1011
BbvCI CCTCAGC 2 cut(s) 290, 806
BbvI GCAGC 3 cut(s) 755, 938, 1110
BccI CCATC 2 cut(s) 25, 214
BceAI ACGGC 4 cut(s) 724, 890, 1459, 1491
BcgI CGANNNNNNTGC 2 cut(s) 141, 175
BciT130I CCWGG 3 cut(s) 275, 797, 895
BciVI GTATCC 1 cut(s) 523
BclI TGATCA 1 cut(s) 513
BcnI CCSGG 6 cut(s) 115, 230, 311, 463, 838, 839
BcoDI GTCTC 1 cut(s) 244
BcuI ACTAGT 1 cut(s) 1192
BfaI CTAG 1 cut(s) 1193
BfmI CTRYAG 1 cut(s) 732
BfoI RGCGCY 2 cut(s) 580, 1110
BfuI GTATCC 1 cut(s) 523
Bme1390I CCNGG 9 cut(s) 115, 230, 275, 311, 463, 797, 838, 839, 895
Bme18I GGWCC 6 cut(s) 34, 226, 822, 1137, 1209, 1240
BmeT110I CYCGRG 4 cut(s) 580, 837, 1226, 1615
BmgBI CACGTC 1 cut(s) 1006
BmiI GGNNCC 6 cut(s) 228, 383, 624, 842, 1021, 1236
BmrFI CCNGG 9 cut(s) 115, 230, 275, 311, 463, 797, 838, 839, 895
BmsI GCATC 1 cut(s) 279
BpiI GAAGAC 3 cut(s) 666, 747, 1478
BplI GAGNNNNNCTC 4 cut(s) 1376, 1408, 1618, 1650
Bpu10I CCTNAGC 2 cut(s) 290, 806
Bpu14I TTCGAA 1 cut(s) 780
BpuEI CTTGAG 3 cut(s) 580, 995, 1389
BpuMI CCSGG 6 cut(s) 115, 230, 311, 463, 838, 839
BsaAI YACGTR 1 cut(s) 775
BsaBI GATNNNNATC 1 cut(s) 968
BsaHI GRCGYC 1 cut(s) 834
BsaJI CCNNGG 6 cut(s) 113, 393, 618, 795, 837, 857
BsaWI WCCGGW 5 cut(s) 1046, 1134, 1206, 1237, 1646
BsaXI ACNNNNNCTCC 4 cut(s) 491, 521, 607, 637
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 1273, 1653
Bse118I RCCGGY 2 cut(s) 1206, 1646
Bse3DI GCAATG 1 cut(s) 453
Bse8I GATNNNNATC 1 cut(s) 968
BseBI CCWGG 3 cut(s) 275, 797, 895
BseDI CCNNGG 6 cut(s) 113, 393, 618, 795, 837, 857
BseGI GGATG 1 cut(s) 270
BseJI GATNNNNATC 1 cut(s) 968
BseLI CCNNNNNNNGG 3 cut(s) 209, 1273, 1653
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 6 cut(s) 304, 388, 659, 775, 797, 1427
BsePI GCGCGC 1 cut(s) 262
BseRI GAGGAG 5 cut(s) 232, 325, 803, 984, 1558
BseSI GKGCMC 1 cut(s) 844
BseXI GCAGC 3 cut(s) 755, 938, 1110
BsgI GTGCAG 2 cut(s) 835, 1109
Bsh1236I CGCG 8 cut(s) 264, 447, 557, 574, 620, 820, 859, 1091
Bsh1285I CGRYCG 1 cut(s) 399
BshFI GGCC 9 cut(s) 249, 278, 560, 794, 842, 873, 1266, 1328, 1421
BshNI GGYRCC 1 cut(s) 381
BshTI ACCGGT 2 cut(s) 1206, 1646
BsiEI CGRYCG 1 cut(s) 399
BsiHKAI GWGCWC 1 cut(s) 1011
BsiHKCI CYCGRG 4 cut(s) 580, 837, 1226, 1615
BsiWI CGTACG 1 cut(s) 973
BslFI GGGAC 3 cut(s) 96, 271, 709
BslI CCNNNNNNNGG 3 cut(s) 209, 1273, 1653
BsmAI GTCTC 1 cut(s) 244
BsmBI CGTCTC 1 cut(s) 244
BsmFI GGGAC 3 cut(s) 96, 271, 709
BsnI GGCC 9 cut(s) 249, 278, 560, 794, 842, 873, 1266, 1328, 1421
BsoBI CYCGRG 4 cut(s) 580, 837, 1226, 1615
Bsp119I TTCGAA 1 cut(s) 780
Bsp120I GGGCCC 1 cut(s) 840
Bsp1286I GDGCHC 2 cut(s) 844, 1011
Bsp143I GATC 7 cut(s) 400, 513, 753, 813, 969, 999, 1019
BspANI GGCC 9 cut(s) 249, 278, 560, 794, 842, 873, 1266, 1328, 1421
BspCNI CTCAG 6 cut(s) 303, 387, 658, 774, 798, 1428
BspFNI CGCG 8 cut(s) 264, 447, 557, 574, 620, 820, 859, 1091
BspLI GGNNCC 6 cut(s) 228, 383, 624, 842, 1021, 1236
BspPI GGATC 3 cut(s) 395, 1014, 1027
BspT104I TTCGAA 1 cut(s) 780
BspT107I GGYRCC 1 cut(s) 381
BsrBI CCGCTC 4 cut(s) 83, 305, 729, 1150
BsrDI GCAATG 1 cut(s) 453
BsrFI RCCGGY 2 cut(s) 1206, 1646
BssAI RCCGGY 2 cut(s) 1206, 1646
BssECI CCNNGG 6 cut(s) 113, 393, 618, 795, 837, 857
BssHII GCGCGC 1 cut(s) 262
BssMI GATC 7 cut(s) 400, 513, 753, 813, 969, 999, 1019
BssNI GRCGYC 1 cut(s) 834
Bst2UI CCWGG 3 cut(s) 275, 797, 895
Bst4CI ACNGT 5 cut(s) 521, 541, 703, 716, 978
BstACI GRCGYC 1 cut(s) 834
BstBAI YACGTR 1 cut(s) 775
BstBI TTCGAA 1 cut(s) 780
BstC8I GCNNGC 8 cut(s) 103, 180, 264, 268, 280, 1148, 1326, 1480
BstDEI CTNAG 6 cut(s) 290, 374, 645, 761, 806, 1436
BstDSI CCRYGG 2 cut(s) 618, 857
BstEII GGTNACC 1 cut(s) 613
BstF5I GGATG 1 cut(s) 270
BstFNI CGCG 8 cut(s) 264, 447, 557, 574, 620, 820, 859, 1091
BstH2I RGCGCY 2 cut(s) 580, 1110
BstHHI GCGC 7 cut(s) 264, 266, 449, 579, 1033, 1100, 1109
BstKTI GATC 7 cut(s) 403, 516, 756, 816, 972, 1002, 1022
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 7 cut(s) 400, 513, 753, 813, 969, 999, 1019
BstMCI CGRYCG 1 cut(s) 399
BstNI CCWGG 3 cut(s) 275, 797, 895
BstPI GGTNACC 1 cut(s) 613
BstSCI CCNGG 9 cut(s) 113, 228, 273, 309, 461, 795, 836, 837, 893
BstSFI CTRYAG 1 cut(s) 732
BstSLI GKGCMC 1 cut(s) 844
BstUI CGCG 8 cut(s) 264, 447, 557, 574, 620, 820, 859, 1091
BstV1I GCAGC 3 cut(s) 755, 938, 1110
BstV2I GAAGAC 3 cut(s) 666, 747, 1478
BstX2I RGATCY 1 cut(s) 1019
BstYI RGATCY 1 cut(s) 1019
BsuI GTATCC 1 cut(s) 523
BsuRI GGCC 9 cut(s) 249, 278, 560, 794, 842, 873, 1266, 1328, 1421
BtgI CCRYGG 2 cut(s) 618, 857
BtrI CACGTC 1 cut(s) 1006
BtsCI GGATG 1 cut(s) 270
BtsI GCAGTG 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 103
Cac8I GCNNGC 8 cut(s) 103, 180, 264, 268, 280, 1148, 1326, 1480
CaiI CAGNNNCTG 2 cut(s) 511, 768
CfoI GCGC 7 cut(s) 264, 266, 449, 579, 1033, 1100, 1109
Cfr10I RCCGGY 2 cut(s) 1206, 1646
Cfr42I CCGCGG 2 cut(s) 621, 860
Cfr9I CCCGGG 1 cut(s) 837
CpoI CGGWCCG 1 cut(s) 1137
CseI GACGC 2 cut(s) 580, 842
Csp6I GTAC 3 cut(s) 898, 974, 1180
CspAI ACCGGT 2 cut(s) 1206, 1646
CspI CGGWCCG 1 cut(s) 1137
CviAII CATG 2 cut(s) 1160, 1270
CviQI GTAC 3 cut(s) 898, 974, 1180
DdeI CTNAG 6 cut(s) 290, 374, 645, 761, 806, 1436
DpnI GATC 7 cut(s) 402, 515, 755, 815, 971, 1001, 1021
DpnII GATC 7 cut(s) 400, 513, 753, 813, 969, 999, 1019
EaeI YGGCCR 4 cut(s) 247, 558, 1264, 1419
EciI GGCGGA 7 cut(s) 101, 133, 239, 309, 418, 615, 866
Eco147I AGGCCT 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 844
Eco47I GGWCC 6 cut(s) 34, 226, 822, 1137, 1209, 1240
Eco57I CTGAAG 1 cut(s) 1042
Eco72I CACGTG 1 cut(s) 775
Eco88I CYCGRG 4 cut(s) 580, 837, 1226, 1615
Eco91I GGTNACC 1 cut(s) 613
EcoO109I RGGNCCY 1 cut(s) 793
EcoO65I GGTNACC 1 cut(s) 613
EcoRII CCWGG 3 cut(s) 273, 795, 893
EcoT38I GRGCYC 1 cut(s) 844
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 2 cut(s) 1163, 1273
FaiI YATR 7 cut(s) 20, 39, 890, 1161, 1271, 1292, 1431
FaqI GGGAC 3 cut(s) 96, 271, 709
FatI CATG 2 cut(s) 1159, 1269
FauI CCCGC 5 cut(s) 734, 864, 1317, 1471, 1655
FbaI TGATCA 1 cut(s) 513
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 844
FspBI CTAG 1 cut(s) 1193
GlaI GCGC 7 cut(s) 263, 265, 448, 578, 1032, 1099, 1108
HaeII RGCGCY 2 cut(s) 580, 1110
HaeIII GGCC 9 cut(s) 249, 278, 560, 794, 842, 873, 1266, 1328, 1421
HgaI GACGC 2 cut(s) 580, 842
HhaI GCGC 7 cut(s) 264, 266, 449, 579, 1033, 1100, 1109
Hin1I GRCGYC 1 cut(s) 834
Hin1II CATG 2 cut(s) 1163, 1273
Hin6I GCGC 7 cut(s) 262, 264, 447, 577, 1031, 1098, 1107
HinP1I GCGC 7 cut(s) 262, 264, 447, 577, 1031, 1098, 1107
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HindIII AAGCTT 1 cut(s) 128
HpaI GTTAAC 1 cut(s) 706
HphI GGTGA 2 cut(s) 607, 1375
Hpy166II GTNNAC 3 cut(s) 706, 900, 916
Hpy188III TCNNGA 4 cut(s) 11, 751, 981, 1406
Hpy8I GTNNAC 3 cut(s) 706, 900, 916
Hpy99I CGWCG 7 cut(s) 148, 151, 163, 491, 673, 926, 1007
HpyAV CCTTC 4 cut(s) 265, 688, 1390, 1570
HpyCH4III ACNGT 5 cut(s) 521, 541, 703, 716, 978
HpyCH4IV ACGT 3 cut(s) 486, 774, 1005
HpyCH4V TGCA 6 cut(s) 101, 270, 282, 852, 1083, 1126
HpyF3I CTNAG 6 cut(s) 290, 374, 645, 761, 806, 1436
HpySE526I ACGT 3 cut(s) 486, 774, 1005
Hsp92I GRCGYC 1 cut(s) 834
Hsp92II CATG 2 cut(s) 1163, 1273
HspAI GCGC 7 cut(s) 262, 264, 447, 577, 1031, 1098, 1107
Ksp22I TGATCA 1 cut(s) 513
KspAI GTTAAC 1 cut(s) 706
KspI CCGCGG 2 cut(s) 621, 860
Kzo9I GATC 7 cut(s) 400, 513, 753, 813, 969, 999, 1019
LmnI GCTCC 3 cut(s) 187, 605, 628
Lsp1109I GCAGC 3 cut(s) 755, 938, 1110
LweI GCATC 1 cut(s) 279
MaeI CTAG 1 cut(s) 1193
MaeII ACGT 3 cut(s) 486, 774, 1005
MaeIII GTNAC 3 cut(s) 584, 613, 1439
MalI GATC 7 cut(s) 402, 515, 755, 815, 971, 1001, 1021
MbiI CCGCTC 4 cut(s) 83, 305, 729, 1150
MboI GATC 7 cut(s) 400, 513, 753, 813, 969, 999, 1019
MboII GAAGA 9 cut(s) 666, 749, 752, 823, 1048, 1483, 1592, 1595, 1598
MflI RGATCY 1 cut(s) 1019
MhlI GDGCHC 2 cut(s) 844, 1011
MlsI TGGCCA 2 cut(s) 1266, 1421
MluCI AATT 5 cut(s) 153, 544, 1300, 1604, 1685
MluNI TGGCCA 2 cut(s) 1266, 1421
MmeI TCCRAC 3 cut(s) 372, 1317, 1533
Mox20I TGGCCA 2 cut(s) 1266, 1421
MscI TGGCCA 2 cut(s) 1266, 1421
MseI TTAA 5 cut(s) 525, 705, 966, 1221, 1373
Msp20I TGGCCA 2 cut(s) 1266, 1421
MspA1I CMGCKG 2 cut(s) 620, 859
MspR9I CCNGG 9 cut(s) 115, 230, 275, 311, 463, 797, 838, 839, 895
MvaI CCWGG 3 cut(s) 275, 797, 895
MvnI CGCG 8 cut(s) 264, 447, 557, 574, 620, 820, 859, 1091
NciI CCSGG 6 cut(s) 115, 230, 311, 463, 838, 839
NdeII GATC 7 cut(s) 400, 513, 753, 813, 969, 999, 1019
NlaIII CATG 2 cut(s) 1163, 1273
NlaIV GGNNCC 6 cut(s) 228, 383, 624, 842, 1021, 1236
NmeAIII GCCGAG 4 cut(s) 457, 675, 1135, 1145
NmuCI GTSAC 2 cut(s) 584, 613
NspV TTCGAA 1 cut(s) 780
PaeR7I CTCGAG 1 cut(s) 580
PauI GCGCGC 1 cut(s) 262
PceI AGGCCT 1 cut(s) 278
PcsI WCGNNNNNNNCGW 4 cut(s) 155, 429, 561, 578
PfeI GAWTC 3 cut(s) 502, 608, 1340
Pfl23II CGTACG 1 cut(s) 973
PflFI GACNNNGTC 2 cut(s) 230, 920
PfoI TCCNGGA 2 cut(s) 309, 461
PinAI ACCGGT 2 cut(s) 1206, 1646
PmaCI CACGTG 1 cut(s) 775
PmlI CACGTG 1 cut(s) 775
Ppu21I YACGTR 1 cut(s) 775
Psp6I CCWGG 3 cut(s) 273, 795, 893
PspCI CACGTG 1 cut(s) 775
PspEI GGTNACC 1 cut(s) 613
PspGI CCWGG 3 cut(s) 273, 795, 893
PspLI CGTACG 1 cut(s) 973
PspN4I GGNNCC 6 cut(s) 228, 383, 624, 842, 1021, 1236
PspOMI GGGCCC 1 cut(s) 840
PspXI VCTCGAGB 1 cut(s) 580
PstNI CAGNNNCTG 2 cut(s) 511, 768
PsuI RGATCY 1 cut(s) 1019
PsyI GACNNNGTC 2 cut(s) 230, 920
PteI GCGCGC 1 cut(s) 262
RsaI GTAC 3 cut(s) 899, 975, 1181
RsaNI GTAC 3 cut(s) 898, 974, 1180
Rsr2I CGGWCCG 1 cut(s) 1137
RsrII CGGWCCG 1 cut(s) 1137
SacII CCGCGG 2 cut(s) 621, 860
SaqAI TTAA 5 cut(s) 525, 705, 966, 1221, 1373
Sau3AI GATC 7 cut(s) 400, 513, 753, 813, 969, 999, 1019
ScrFI CCNGG 9 cut(s) 115, 230, 275, 311, 463, 797, 838, 839, 895
SduI GDGCHC 2 cut(s) 844, 1011
SfaNI GCATC 1 cut(s) 279
SfcI CTRYAG 1 cut(s) 732
Sfr274I CTCGAG 1 cut(s) 580
Sfr303I CCGCGG 2 cut(s) 621, 860
SfuI TTCGAA 1 cut(s) 780
SgrBI CCGCGG 2 cut(s) 621, 860
SinI GGWCC 6 cut(s) 34, 226, 822, 1137, 1209, 1240
SlaI CTCGAG 1 cut(s) 580
SmaI CCCGGG 1 cut(s) 839
SmlI CTYRAG 4 cut(s) 580, 595, 1010, 1404
SmoI CTYRAG 4 cut(s) 580, 595, 1010, 1404
SpeI ACTAGT 1 cut(s) 1192
SrfI GCCCGGGC 1 cut(s) 839
Sse9I AATT 5 cut(s) 153, 544, 1300, 1604, 1685
SseBI AGGCCT 1 cut(s) 278
SspMI CTAG 1 cut(s) 1193
StuI AGGCCT 1 cut(s) 278
StyD4I CCNGG 9 cut(s) 113, 228, 273, 309, 461, 795, 836, 837, 893
TaaI ACNGT 5 cut(s) 521, 541, 703, 716, 978
TaiI ACGT 3 cut(s) 489, 777, 1008
TaqII GACCGA 2 cut(s) 385, 601
TasI AATT 5 cut(s) 153, 544, 1300, 1604, 1685
TauI GCSGC 8 cut(s) 86, 178, 325, 560, 577, 623, 1117, 1331
TfiI GAWTC 3 cut(s) 502, 608, 1340
Tru1I TTAA 5 cut(s) 525, 705, 966, 1221, 1373
Tru9I TTAA 5 cut(s) 525, 705, 966, 1221, 1373
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 2 cut(s) 584, 613
TseI GCWGC 3 cut(s) 768, 926, 1123
Tsp45I GTSAC 2 cut(s) 584, 613
TspDTI ATGAA 2 cut(s) 538, 621
TspGWI ACGGA 5 cut(s) 147, 328, 657, 711, 910
TspMI CCCGGG 1 cut(s) 837
TspRI CASTG 1 cut(s) 103
Tth111I GACNNNGTC 2 cut(s) 230, 920
VpaK11BI GGWCC 6 cut(s) 34, 226, 822, 1137, 1209, 1240
XapI RAATTY 2 cut(s) 544, 1685
XhoI CTCGAG 1 cut(s) 580
XmaI CCCGGG 1 cut(s) 837
XspI CTAG 1 cut(s) 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.