Rh2DG643800

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012888
Physical Location & Seq
Forward (+)
87074793 .. 87076466
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG643800.1

Sequence Viewer

Length: 1674 bp
ATGGAGTTTAAAGGGGGTCTGATGAGATTAGTAGTCCACAAATCCAAAGAAACCAATTGCGTTTACGACGCCGGAGATAGATTAACAGAGATAATAAGCTCTGCTCGGAGCCCAAGCCGGAGATGGAAGCTGTTTCACAGGTCCTCATCTGCAATTCCATCAAAGCCTTCAAAGCCTCAAATCCCAAGAGAGTTCATTTGCCCCATTTCCGGGTCATTAATGGGCGACCCGGTTATAGTTTCCTCGGGTCACACCTTCGAACGCGTCTGCGTCCAAGTCTGCAAAGCCCTCAACTTCACCCCTAATCTCGTGGACTCGCCCCCACCTGATCTCACCTCCGTCATCCCCAACCTCGCCCTCAAGTCCACCATCCTCAACTGGTGTCAAAATTACTCGATTGCCCCTCCCAAGCCACTCGACTCTATCTCCGCCGAGAAGCTCGTCCGTACATCCATGGCGTCCGGCCGCCGGAAAGTCTCGGTTTCCGAGAACGAGTCGCTGATCCGAGGCGTGGAGGAGACGCCCAACGTGAGCTTCAACCACGCGGCGACCGAGTTGACTCGTCGGCCGAGTCACTTCCACTCCAGCTCCGACGAGTCGGTCAGCGATGCCGCGTTGACCCCGCCGCTGCCGTTCTCGACTCAGCCGAGCTGCTACTCGTCGCGTTCGTCTTCGTGTTCGGAAATCGAAGCCCTGACGAATTCGAACGAGGAAGACGAAGTCTTGGCCCGGATCAGAAGCGTCCACGTGTCCGAAATCGAGGAGGCTCTGACGTGGCTGAGGAAGATCACACGGACCAGAGAGGACGCCCGGGCCCACCTCTGCAGTCCTCGGCTCCTCTCCGCCTTGAGACCACTGGTCGTTTCCAGGTACGCCAATATTCAGGTGAACTCAGTCGCAGTTTTGGTTAACCTTTCGCTGGAAAAGTCGAACAAGATTAAGATCGTACGGTCAGGAATTCTCCCGCCTTTGATTGATGTGCTTAAGGCGGGAATGGCGCCTGAGGCGCAGGAGCACGCCTCCGGTGTGGTCTTTAGCTTGGCGCTGGATGACGATTGCAAGACGGCTATCGGAGTATTGGGGGCTCTGCCGCCTTTGCTTCACTTGCTGAACTCGGAGGGCGAGCGGACTCGGCATGACTCGGCACTTGCTTTGTACCATTTGACATTGGTTCAGAGTAACCGGTCCAAGTTGGTTAAGATCGGGTCGGTTCCGGTTCTTTTGAGGATGGTGAAGTCGGGTCACATGACGGGTCGGGTGTTGCTCACATTGTGCAACTTGAGTTCGTGTGCGGACGGGCGGGCGGCGATGTTGGATTCAGGTGGGGTGGAGTGTTTGGTGGGGGTGTTGAGGGGGAATGAGTTTGATTCCCGGTCGACTCAGGAGAGTTGTGTGGCTACATTGTATGGGTTGAGTCGTGGGGGTTTGAGGTTTAAGGGGTTGGCGAAGGCGGCCGGGGCGGTGGAGGTGTTGAGGGAGGTGGAGAAGAGTGGGAGTGAGAGAGGGAGGGAGAAGGCGAGGAGAATGTTGGAGATGATGAGAGGGAGAGAGAAGGAGGAAGAGGAGGAAGTGGATTGGGAGGAGTTGCTTGACTCGGGGTTGGGAAGTCGAACTAGGCTTCAGTCCGGTTGTGGTGCATTGGGTGGGTCTTGTGTTAATTCGTCTGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

557

Amino Acids

60.62

Weight (kDa)

8.44

Isoelectric Point (pI)

55.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 61 - 130 3e-10 U-box domain
ARM_PUB PF25598 251 - 448 1.3e-08 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 351 - 468 1.6e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40140
fragaria_vesca FvH4_6g50090
malus_domestica MD09G1039200.v1.1 MD17G1040200.v1.1
prunus_persica Prupe.3G278800_v2.0.a1
pyrus_communis pycom111g03090 pycom17g03600
rosa_chinensis RchiOBHm_Chr2g0170361
rosa_laevigata RLG00000021949
rosa_multiflora Rmu_co8477427.1_g000001 Rmu_sc0011601.1_g000012
rosa_roxburghii Rroxscaffold_2G00081400
rosa_rugosa Rorug02G0548500
rosa_samantha Rh2AG619900 Rh2BG630900 Rh2CG600900 Rh2DG643800
rosa_wichuraiana Rw2G051360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 599
AccB1I GGYRCC 1 cut(s) 997
AccBSI CCGCTC 1 cut(s) 1126
AccI GTMKAC 1 cut(s) 1376
AccII CGCG 4 cut(s) 264, 545, 614, 664
AclWI GGATC 2 cut(s) 496, 740
AcoI YGGCCR 3 cut(s) 463, 566, 1452
AcsI RAATTY 2 cut(s) 700, 957
AcuI CTGAAG 1 cut(s) 1604
AcvI CACGTG 1 cut(s) 748
AcyI GRCGYC 5 cut(s) 69, 458, 521, 807, 998
AdeI CACNNNGTG 1 cut(s) 1272
AfaI GTAC 4 cut(s) 448, 872, 948, 1157
AfiI CCNNNNNNNGG 5 cut(s) 209, 210, 468, 511, 919
AflII CTTAAG 1 cut(s) 983
AflIII ACRYGT 2 cut(s) 262, 747
AgeI ACCGGT 1 cut(s) 1182
AgsI TTSAA 2 cut(s) 171, 538
AhdI GACNNNNNGTC 1 cut(s) 857
AjiI CACGTC 1 cut(s) 774
AjnI CCWGG 1 cut(s) 866
AjuI GAANNNNNNNTTGG 2 cut(s) 518, 550
AluBI AGCT 7 cut(s) 99, 130, 439, 534, 588, 651, 1038
AluI AGCT 7 cut(s) 99, 130, 439, 534, 588, 651, 1038
Alw21I GWGCWC 1 cut(s) 1017
Alw26I GTCTC 3 cut(s) 481, 512, 844
AlwI GGATC 2 cut(s) 496, 740
Ama87I CYCGRG 3 cut(s) 244, 810, 1594
AoxI GGCC 5 cut(s) 463, 566, 726, 813, 1452
ApaI GGGCCC 1 cut(s) 817
ApeKI GCWGC 2 cut(s) 628, 651
ApoI RAATTY 2 cut(s) 700, 957
AseI ATTAAT 1 cut(s) 218
AsiGI ACCGGT 1 cut(s) 1182
AspLEI GCGC 3 cut(s) 1000, 1009, 1045
AspS9I GGNCC 6 cut(s) 141, 727, 795, 813, 814, 1185
AsuC2I CCSGG 7 cut(s) 211, 230, 730, 811, 812, 1372, 1456
AsuHPI GGTGA 4 cut(s) 289, 325, 898, 1243
AsuII TTCGAA 2 cut(s) 258, 704
AvaI CYCGRG 3 cut(s) 244, 810, 1594
AvaII GGWCC 3 cut(s) 141, 795, 1185
AxyI CCTNAGG 1 cut(s) 1002
BaeGI GKGCMC 1 cut(s) 817
BanI GGYRCC 1 cut(s) 997
BanII GRGCYC 3 cut(s) 113, 817, 1087
BauI CACGAG 1 cut(s) 308
BbrPI CACGTG 1 cut(s) 748
BbsI GAAGAC 2 cut(s) 663, 720
Bbv12I GWGCWC 1 cut(s) 1017
BbvCI CCTCAGC 1 cut(s) 779
BbvI GCAGC 2 cut(s) 615, 638
BccI CCATC 4 cut(s) 117, 166, 377, 1222
BceAI ACGGC 2 cut(s) 616, 1080
BciT130I CCWGG 1 cut(s) 868
BcnI CCSGG 7 cut(s) 211, 230, 730, 811, 812, 1372, 1456
BcoDI GTCTC 3 cut(s) 481, 512, 844
BfaI CTAG 1 cut(s) 1614
BfmI CTRYAG 1 cut(s) 823
BfoI RGCGCY 2 cut(s) 1001, 1046
BfrI CTTAAG 1 cut(s) 983
BisI GCNGC 9 cut(s) 466, 546, 612, 626, 629, 652, 1091, 1305, 1452
BlsI GCNGC 9 cut(s) 467, 547, 613, 627, 630, 653, 1092, 1306, 1453
Bme1390I CCNGG 8 cut(s) 211, 230, 730, 811, 812, 868, 1372, 1456
Bme18I GGWCC 3 cut(s) 141, 795, 1185
BmeRI GACNNNNNGTC 1 cut(s) 857
BmeT110I CYCGRG 3 cut(s) 244, 810, 1594
BmgBI CACGTC 1 cut(s) 774
BmgT120I GGNCC 6 cut(s) 141, 727, 795, 813, 814, 1185
BmiI GGNNCC 5 cut(s) 110, 815, 836, 999, 1212
BmrFI CCNGG 8 cut(s) 211, 230, 730, 811, 812, 868, 1372, 1456
BmsI GCATC 1 cut(s) 598
BpiI GAAGAC 2 cut(s) 663, 720
BplI GAGNNNNNCTC 2 cut(s) 1004, 1036
BpmI CTGGAG 1 cut(s) 568
Bpu10I CCTNAGC 1 cut(s) 779
Bpu14I TTCGAA 2 cut(s) 258, 704
BpuEI CTTGAG 3 cut(s) 344, 868, 1300
BpuMI CCSGG 7 cut(s) 211, 230, 730, 811, 812, 1372, 1456
BsaAI YACGTR 1 cut(s) 748
BsaBI GATNNNNATC 1 cut(s) 941
BsaHI GRCGYC 5 cut(s) 69, 458, 521, 807, 998
BsaI GGTCTC 1 cut(s) 844
BsaJI CCNNGG 6 cut(s) 243, 453, 505, 810, 830, 1455
BsaWI WCCGGW 4 cut(s) 1022, 1182, 1213, 1625
BsaXI ACNNNNNCTCC 6 cut(s) 410, 440, 566, 572, 596, 602
Bsc4I CCNNNNNNNGG 5 cut(s) 209, 210, 468, 511, 919
Bse118I RCCGGY 1 cut(s) 1182
Bse1I ACTGG 2 cut(s) 383, 861
Bse21I CCTNAGG 1 cut(s) 1002
Bse8I GATNNNNATC 1 cut(s) 941
BseBI CCWGG 1 cut(s) 868
BseDI CCNNGG 6 cut(s) 243, 453, 505, 810, 830, 1455
BseGI GGATG 5 cut(s) 342, 369, 449, 1054, 1233
BseJI GATNNNNATC 1 cut(s) 941
BseLI CCNNNNNNNGG 5 cut(s) 209, 210, 468, 511, 919
BseMII CTCAG 6 cut(s) 656, 770, 906, 993, 1394, 1656
BseNI ACTGG 2 cut(s) 383, 861
BseRI GAGGAG 6 cut(s) 530, 776, 827, 1534, 1577, 1595
BseSI GKGCMC 1 cut(s) 817
BseX3I CGGCCG 3 cut(s) 463, 566, 1452
BseXI GCAGC 2 cut(s) 615, 638
Bsh1236I CGCG 4 cut(s) 264, 545, 614, 664
Bsh1285I CGRYCG 5 cut(s) 466, 552, 569, 1376, 1455
BshFI GGCC 5 cut(s) 465, 568, 728, 815, 1454
BshNI GGYRCC 1 cut(s) 997
BshTI ACCGGT 1 cut(s) 1182
BsiEI CGRYCG 5 cut(s) 466, 552, 569, 1376, 1455
BsiHKAI GWGCWC 1 cut(s) 1017
BsiHKCI CYCGRG 3 cut(s) 244, 810, 1594
BsiWI CGTACG 1 cut(s) 946
BslI CCNNNNNNNGG 5 cut(s) 209, 210, 468, 511, 919
BsmAI GTCTC 3 cut(s) 481, 512, 844
BsmBI CGTCTC 1 cut(s) 512
BsnI GGCC 5 cut(s) 465, 568, 728, 815, 1454
Bso31I GGTCTC 1 cut(s) 844
BsoBI CYCGRG 3 cut(s) 244, 810, 1594
Bsp119I TTCGAA 2 cut(s) 258, 704
Bsp120I GGGCCC 1 cut(s) 813
Bsp1286I GDGCHC 4 cut(s) 113, 817, 1017, 1087
Bsp143I GATC 6 cut(s) 328, 501, 732, 786, 942, 1200
Bsp19I CCATGG 1 cut(s) 453
BspANI GGCC 5 cut(s) 465, 568, 728, 815, 1454
BspCNI CTCAG 6 cut(s) 655, 771, 905, 994, 1393, 1657
BspFNI CGCG 4 cut(s) 264, 545, 614, 664
BspLI GGNNCC 5 cut(s) 110, 815, 836, 999, 1212
BspMAI CTGCAG 1 cut(s) 827
BspPI GGATC 2 cut(s) 496, 740
BspT104I TTCGAA 2 cut(s) 258, 704
BspT107I GGYRCC 1 cut(s) 997
BspTI CTTAAG 1 cut(s) 983
BspTNI GGTCTC 1 cut(s) 844
BsrBI CCGCTC 1 cut(s) 1126
BsrFI RCCGGY 1 cut(s) 1182
BsrI ACTGG 2 cut(s) 383, 861
BssAI RCCGGY 1 cut(s) 1182
BssECI CCNNGG 6 cut(s) 243, 453, 505, 810, 830, 1455
BssMI GATC 6 cut(s) 328, 501, 732, 786, 942, 1200
BssNI GRCGYC 5 cut(s) 69, 458, 521, 807, 998
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 868
Bst4CI ACNGT 1 cut(s) 951
Bst6I CTCTTC 2 cut(s) 1481, 1554
BstACI GRCGYC 5 cut(s) 69, 458, 521, 807, 998
BstAFI CTTAAG 1 cut(s) 983
BstBAI YACGTR 1 cut(s) 748
BstBI TTCGAA 2 cut(s) 258, 704
BstC8I GCNNGC 3 cut(s) 1017, 1124, 1302
BstDEI CTNAG 6 cut(s) 642, 779, 892, 1002, 1380, 1665
BstDSI CCRYGG 1 cut(s) 453
BstF5I GGATG 5 cut(s) 342, 369, 449, 1054, 1233
BstFNI CGCG 4 cut(s) 264, 545, 614, 664
BstH2I RGCGCY 2 cut(s) 1001, 1046
BstHHI GCGC 3 cut(s) 1000, 1009, 1045
BstKTI GATC 6 cut(s) 331, 504, 735, 789, 945, 1203
BstMAI GTCTC 3 cut(s) 481, 512, 844
BstMBI GATC 6 cut(s) 328, 501, 732, 786, 942, 1200
BstMCI CGRYCG 5 cut(s) 466, 552, 569, 1376, 1455
BstMWI GCNNNNNNNGC 9 cut(s) 172, 995, 1004, 1006, 1096, 1105, 1132, 1451, 1457
BstNI CCWGG 1 cut(s) 868
BstSCI CCNGG 8 cut(s) 209, 228, 728, 809, 810, 866, 1370, 1454
BstSFI CTRYAG 1 cut(s) 823
BstSLI GKGCMC 1 cut(s) 817
BstUI CGCG 4 cut(s) 264, 545, 614, 664
BstV1I GCAGC 2 cut(s) 615, 638
BstV2I GAAGAC 2 cut(s) 663, 720
BstZI CGGCCG 3 cut(s) 463, 566, 1452
Bsu36I CCTNAGG 1 cut(s) 1002
BsuRI GGCC 5 cut(s) 465, 568, 728, 815, 1454
BtgI CCRYGG 1 cut(s) 453
BtgZI GCGATG 2 cut(s) 621, 1322
BtrI CACGTC 1 cut(s) 774
BtsCI GGATG 5 cut(s) 342, 369, 449, 1054, 1233
BtsIMutI CAGTG 1 cut(s) 854
Cac8I GCNNGC 3 cut(s) 1017, 1124, 1302
CfoI GCGC 3 cut(s) 1000, 1009, 1045
Cfr10I RCCGGY 1 cut(s) 1182
Cfr13I GGNCC 6 cut(s) 141, 727, 795, 813, 814, 1185
Cfr9I CCCGGG 1 cut(s) 810
CseI GACGC 7 cut(s) 77, 253, 259, 447, 529, 730, 815
Csp6I GTAC 4 cut(s) 447, 871, 947, 1156
CspAI ACCGGT 1 cut(s) 1182
CviAII CATG 3 cut(s) 454, 1136, 1246
CviQI GTAC 4 cut(s) 447, 871, 947, 1156
DdeI CTNAG 6 cut(s) 642, 779, 892, 1002, 1380, 1665
DinI GGCGCC 1 cut(s) 999
DpnI GATC 6 cut(s) 330, 503, 734, 788, 944, 1202
DpnII GATC 6 cut(s) 328, 501, 732, 786, 942, 1200
DraI TTTAAA 1 cut(s) 10
DraIII CACNNNGTG 1 cut(s) 1272
DrdI GACNNNNNNGTC 1 cut(s) 599
DriI GACNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 599
EaeI YGGCCR 3 cut(s) 463, 566, 1452
EagI CGGCCG 3 cut(s) 463, 566, 1452
Eam1104I CTCTTC 2 cut(s) 1481, 1554
Eam1105I GACNNNNNGTC 1 cut(s) 857
EarI CTCTTC 2 cut(s) 1481, 1554
EciI GGCGGA 2 cut(s) 418, 832
EclXI CGGCCG 3 cut(s) 463, 566, 1452
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 3 cut(s) 113, 817, 1087
Eco31I GGTCTC 1 cut(s) 844
Eco47I GGWCC 3 cut(s) 141, 795, 1185
Eco52I CGGCCG 3 cut(s) 463, 566, 1452
Eco57I CTGAAG 1 cut(s) 1604
Eco72I CACGTG 1 cut(s) 748
Eco81I CCTNAGG 1 cut(s) 1002
Eco88I CYCGRG 3 cut(s) 244, 810, 1594
EcoO109I RGGNCCY 1 cut(s) 141
EcoRI GAATTC 2 cut(s) 700, 957
EcoRII CCWGG 1 cut(s) 866
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 3 cut(s) 113, 817, 1087
EgeI GGCGCC 1 cut(s) 999
EheI GGCGCC 1 cut(s) 999
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 512
FaeI CATG 3 cut(s) 457, 1139, 1249
FaiI YATR 5 cut(s) 236, 455, 1137, 1247, 1407
FatI CATG 3 cut(s) 453, 1135, 1245
FauI CCCGC 4 cut(s) 630, 972, 982, 1293
FblI GTMKAC 1 cut(s) 1376
Fnu4HI GCNGC 9 cut(s) 466, 546, 612, 626, 629, 652, 1091, 1305, 1452
FokI GGATG 5 cut(s) 329, 356, 436, 1061, 1240
FriOI GRGCYC 3 cut(s) 113, 817, 1087
Fsp4HI GCNGC 9 cut(s) 466, 546, 612, 626, 629, 652, 1091, 1305, 1452
FspBI CTAG 1 cut(s) 1614
GlaI GCGC 3 cut(s) 999, 1008, 1044
GluI GCNGC 9 cut(s) 466, 546, 612, 626, 629, 652, 1091, 1305, 1452
GsuI CTGGAG 1 cut(s) 568
HaeII RGCGCY 2 cut(s) 1001, 1046
HaeIII GGCC 5 cut(s) 465, 568, 728, 815, 1454
HgaI GACGC 7 cut(s) 77, 253, 259, 447, 529, 730, 815
HhaI GCGC 3 cut(s) 1000, 1009, 1045
Hin1I GRCGYC 5 cut(s) 69, 458, 521, 807, 998
Hin1II CATG 3 cut(s) 457, 1139, 1249
Hin6I GCGC 3 cut(s) 998, 1007, 1043
HinP1I GCGC 3 cut(s) 998, 1007, 1043
HincII GTYRAC 4 cut(s) 558, 618, 910, 1377
HindII GTYRAC 4 cut(s) 558, 618, 910, 1377
HpaI GTTAAC 1 cut(s) 910
HphI GGTGA 4 cut(s) 289, 325, 898, 1243
Hpy188III TCNNGA 3 cut(s) 637, 954, 1382
Hpy99I CGWCG 4 cut(s) 71, 567, 596, 664
HpyAV CCTTC 5 cut(s) 177, 265, 1441, 1507, 1546
HpyCH4III ACNGT 1 cut(s) 951
HpyCH4IV ACGT 3 cut(s) 528, 747, 773
HpyCH4V TGCA 6 cut(s) 152, 282, 825, 1059, 1275, 1637
HpyF10VI GCNNNNNNNGC 9 cut(s) 172, 995, 1004, 1006, 1096, 1105, 1132, 1451, 1457
HpyF3I CTNAG 6 cut(s) 642, 779, 892, 1002, 1380, 1665
HpySE526I ACGT 3 cut(s) 528, 747, 773
Hsp92I GRCGYC 5 cut(s) 69, 458, 521, 807, 998
Hsp92II CATG 3 cut(s) 457, 1139, 1249
HspAI GCGC 3 cut(s) 998, 1007, 1043
KasI GGCGCC 1 cut(s) 997
KspAI GTTAAC 1 cut(s) 910
Kzo9I GATC 6 cut(s) 328, 501, 732, 786, 942, 1200
LmnI GCTCC 4 cut(s) 108, 593, 840, 1012
Lsp1109I GCAGC 2 cut(s) 615, 638
LweI GCATC 1 cut(s) 598
MaeI CTAG 1 cut(s) 1614
MaeII ACGT 3 cut(s) 528, 747, 773
MaeIII GTNAC 4 cut(s) 248, 572, 1178, 1241
MalI GATC 6 cut(s) 330, 503, 734, 788, 944, 1202
MbiI CCGCTC 1 cut(s) 1126
MboI GATC 6 cut(s) 328, 501, 732, 786, 942, 1200
MboII GAAGA 5 cut(s) 663, 725, 796, 1498, 1571
MfeI CAATTG 1 cut(s) 55
MhlI GDGCHC 4 cut(s) 113, 817, 1017, 1087
MluCI AATT 6 cut(s) 55, 153, 388, 700, 957, 1657
MluI ACGCGT 1 cut(s) 262
Mly113I GGCGCC 1 cut(s) 998
MmeI TCCRAC 3 cut(s) 615, 1293, 1509
MseI TTAA 9 cut(s) 9, 83, 218, 909, 939, 984, 1197, 1434, 1656
MspA1I CMGCKG 1 cut(s) 628
MspCI CTTAAG 1 cut(s) 983
MspR9I CCNGG 8 cut(s) 211, 230, 730, 811, 812, 868, 1372, 1456
MunI CAATTG 1 cut(s) 55
MvaI CCWGG 1 cut(s) 868
MvnI CGCG 4 cut(s) 264, 545, 614, 664
MwoI GCNNNNNNNGC 9 cut(s) 172, 995, 1004, 1006, 1096, 1105, 1132, 1451, 1457
NarI GGCGCC 1 cut(s) 998
NciI CCSGG 7 cut(s) 211, 230, 730, 811, 812, 1372, 1456
NcoI CCATGG 1 cut(s) 453
NdeII GATC 6 cut(s) 328, 501, 732, 786, 942, 1200
NlaIII CATG 3 cut(s) 457, 1139, 1249
NlaIV GGNNCC 5 cut(s) 110, 815, 836, 999, 1212
NmeAIII GCCGAG 6 cut(s) 457, 594, 672, 811, 1111, 1121
NmuCI GTSAC 3 cut(s) 248, 572, 1241
NspV TTCGAA 2 cut(s) 258, 704
PcsI WCGNNNNNNNCGW 4 cut(s) 549, 644, 665, 714
PfeI GAWTC 2 cut(s) 1316, 1367
Pfl23II CGTACG 1 cut(s) 946
PflFI GACNNNGTC 1 cut(s) 719
PinAI ACCGGT 1 cut(s) 1182
PkrI GCNGC 9 cut(s) 467, 547, 613, 627, 630, 653, 1092, 1306, 1453
PluTI GGCGCC 1 cut(s) 1001
PmaCI CACGTG 1 cut(s) 748
PmlI CACGTG 1 cut(s) 748
Ppu21I YACGTR 1 cut(s) 748
PpuMI RGGWCCY 1 cut(s) 141
PshBI ATTAAT 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 866
PspCI CACGTG 1 cut(s) 748
PspGI CCWGG 1 cut(s) 866
PspLI CGTACG 1 cut(s) 946
PspN4I GGNNCC 5 cut(s) 110, 815, 836, 999, 1212
PspOMI GGGCCC 1 cut(s) 813
PspPI GGNCC 6 cut(s) 141, 727, 795, 813, 814, 1185
PspPPI RGGWCCY 1 cut(s) 141
PstI CTGCAG 1 cut(s) 827
PsyI GACNNNGTC 1 cut(s) 719
RsaI GTAC 4 cut(s) 448, 872, 948, 1157
RsaNI GTAC 4 cut(s) 447, 871, 947, 1156
SalI GTCGAC 1 cut(s) 1375
SaqAI TTAA 9 cut(s) 9, 83, 218, 909, 939, 984, 1197, 1434, 1656
SatI GCNGC 9 cut(s) 466, 546, 612, 626, 629, 652, 1091, 1305, 1452
Sau3AI GATC 6 cut(s) 328, 501, 732, 786, 942, 1200
Sau96I GGNCC 6 cut(s) 141, 727, 795, 813, 814, 1185
ScrFI CCNGG 8 cut(s) 211, 230, 730, 811, 812, 868, 1372, 1456
SduI GDGCHC 4 cut(s) 113, 817, 1017, 1087
SfaNI GCATC 1 cut(s) 598
SfcI CTRYAG 1 cut(s) 823
SfoI GGCGCC 1 cut(s) 999
SfuI TTCGAA 2 cut(s) 258, 704
SinI GGWCC 3 cut(s) 141, 795, 1185
SmaI CCCGGG 1 cut(s) 812
SmlI CTYRAG 4 cut(s) 359, 847, 983, 1279
SmoI CTYRAG 4 cut(s) 359, 847, 983, 1279
SrfI GCCCGGGC 1 cut(s) 812
Sse9I AATT 6 cut(s) 55, 153, 388, 700, 957, 1657
SspDI GGCGCC 1 cut(s) 997
SspI AATATT 1 cut(s) 880
SspMI CTAG 1 cut(s) 1614
StyD4I CCNGG 8 cut(s) 209, 228, 728, 809, 810, 866, 1370, 1454
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 1 cut(s) 951
TaiI ACGT 3 cut(s) 531, 750, 776
TaqII GACCGA 2 cut(s) 566, 589
TasI AATT 6 cut(s) 55, 153, 388, 700, 957, 1657
TauI GCSGC 7 cut(s) 468, 548, 614, 628, 1093, 1307, 1454
TfiI GAWTC 2 cut(s) 1316, 1367
Tru1I TTAA 9 cut(s) 9, 83, 218, 909, 939, 984, 1197, 1434, 1656
Tru9I TTAA 9 cut(s) 9, 83, 218, 909, 939, 984, 1197, 1434, 1656
TscAI CASTG 1 cut(s) 861
TseFI GTSAC 3 cut(s) 248, 572, 1241
TseI GCWGC 2 cut(s) 628, 651
Tsp45I GTSAC 3 cut(s) 248, 572, 1241
TspDTI ATGAA 1 cut(s) 184
TspGWI ACGGA 3 cut(s) 328, 434, 808
TspMI CCCGGG 1 cut(s) 810
TspRI CASTG 1 cut(s) 861
Tth111I GACNNNGTC 1 cut(s) 719
Vha464I CTTAAG 1 cut(s) 983
VpaK11BI GGWCC 3 cut(s) 141, 795, 1185
VspI ATTAAT 1 cut(s) 218
XapI RAATTY 2 cut(s) 700, 957
XcmI CCANNNNNNNNNTGG 1 cut(s) 120
XmaI CCCGGG 1 cut(s) 810
XmiI GTMKAC 1 cut(s) 1376
XspI CTAG 1 cut(s) 1614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.