Rmu_sc0011601.1_g000012

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011601.1
OG0012888
Physical Location & Seq
Forward (+)
22286 .. 23702
1417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011601.1_g000012.1.cds

Sequence Viewer

Length: 1248 bp
atggagtttaaagacggtctgatgagattagtagtccacaaatccaaagaaaccagttgcgtttacgacaccggagatagattaacagagataataagctctgctcggagcccaagccggagatggaagctgtttcacaggtcctcatctgcaattccatcaaagccttcaaagcctcaaatcccaagagagttcatttgccccatttccgggtcattaatgggcgacccggttatagtttcctcgggtcacaccttcgaacgcgtctgcgtccaagtctgcaaagccctcaacttcacccctaatctcgtggactcgcccccacctgatctcacctccgtcatccccaacctcgccctcaagtccaccatcctcaactggtgtcaaaattactcgattgcccctcccaagccactcgactctatctccgccgataagctcgtccgtacatccatggcgtccggccaccggaaagtctcggtttccgagaacgagtcgctgatccgaggcgtggaggagacgcctgacgtgagcttcaaccacgcggcgaccgagttgactcgtcggcctagtcacttccactccagctccgacgagtcggtcagcgacgccgcgttgaccccgccgctgccgttctcgactcagccgagctgctactcgtcgcgttcgtcttcgtgttcggaaatcgaagccccgacgaattcgaacgaactctgtaaatatgcgtggcaaaagaggtatggtttcgggagtaaccggtccaagttggttaagatcgggtcggttccggttcttttgaggatggtgaagtcgggtcacatgacgggtcgggtgttgctcacattgtgcaacttgagttcgtgtgcggacgggcgggcggcgatgttggattcgggtggggtggagtgtttggtgggggtgttgagggggaatgagtttgattcccggtcgactcaggagagttgtgtggctacattgtatgggttgagtcgtgggggtttgaggtttaaggggctggcgaaggcggccgaggcagtggaggtgttgagggaagtggagaagagtgggagtgagagagggagggagaaggcgaggaggatgttggagatgatgagagggagggagaaggaggaggaggaggaagtggattgggaggagttgcttgactcggggttgggaagtcgaactaggcttcagtccggttgtggtgcattgggtgggtcttgtgttaattcgtccgagttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

415

Amino Acids

45.32

Weight (kDa)

8.34

Isoelectric Point (pI)

56.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40140
fragaria_vesca FvH4_6g50090
malus_domestica MD09G1039200.v1.1 MD17G1040200.v1.1
prunus_persica Prupe.3G278800_v2.0.a1
pyrus_communis pycom111g03090 pycom17g03600
rosa_chinensis RchiOBHm_Chr2g0170361
rosa_laevigata RLG00000021949
rosa_multiflora Rmu_co8477427.1_g000001 Rmu_sc0011601.1_g000012
rosa_roxburghii Rroxscaffold_2G00081400
rosa_rugosa Rorug02G0548500
rosa_samantha Rh2AG619900 Rh2BG630900 Rh2CG600900 Rh2DG643800
rosa_wichuraiana Rw2G051360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 599
AccI GTMKAC 1 cut(s) 950
AccII CGCG 4 cut(s) 264, 545, 614, 664
AciI CCGC 9 cut(s) 429, 545, 612, 623, 626, 866, 874, 878, 1025
AclWI GGATC 1 cut(s) 496
AcoI YGGCCR 2 cut(s) 463, 1026
AcsI RAATTY 1 cut(s) 700
AcuI CTGAAG 1 cut(s) 1178
AcyI GRCGYC 3 cut(s) 458, 521, 609
AdeI CACNNNGTG 1 cut(s) 846
AfaI GTAC 1 cut(s) 448
AfiI CCNNNNNNNGG 4 cut(s) 209, 210, 468, 511
AflIII ACRYGT 1 cut(s) 262
AgeI ACCGGT 1 cut(s) 756
AgsI TTSAA 2 cut(s) 171, 538
AjiI CACGTC 1 cut(s) 529
AluBI AGCT 6 cut(s) 99, 130, 439, 534, 588, 651
AluI AGCT 6 cut(s) 99, 130, 439, 534, 588, 651
Alw26I GTCTC 2 cut(s) 481, 512
AlwI GGATC 1 cut(s) 496
Ama87I CYCGRG 2 cut(s) 244, 1168
AoxI GGCC 3 cut(s) 463, 566, 1026
ApeKI GCWGC 2 cut(s) 628, 651
ApoI RAATTY 1 cut(s) 700
AseI ATTAAT 1 cut(s) 218
AsiGI ACCGGT 1 cut(s) 756
AspS9I GGNCC 2 cut(s) 141, 759
AsuC2I CCSGG 3 cut(s) 211, 230, 946
AsuHPI GGTGA 3 cut(s) 289, 325, 817
AsuII TTCGAA 2 cut(s) 258, 704
AvaI CYCGRG 2 cut(s) 244, 1168
AvaII GGWCC 2 cut(s) 141, 759
BanII GRGCYC 1 cut(s) 113
BauI CACGAG 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 663
BbvI GCAGC 2 cut(s) 615, 638
BccI CCATC 4 cut(s) 117, 166, 377, 796
BceAI ACGGC 1 cut(s) 616
BcnI CCSGG 3 cut(s) 211, 230, 946
BcoDI GTCTC 2 cut(s) 481, 512
BfaI CTAG 2 cut(s) 570, 1188
BisI GCNGC 7 cut(s) 546, 612, 626, 629, 652, 879, 1026
BlsI GCNGC 7 cut(s) 547, 613, 627, 630, 653, 880, 1027
Bme1390I CCNGG 3 cut(s) 211, 230, 946
Bme18I GGWCC 2 cut(s) 141, 759
BmeT110I CYCGRG 2 cut(s) 244, 1168
BmgBI CACGTC 1 cut(s) 529
BmgT120I GGNCC 2 cut(s) 141, 759
BmiI GGNNCC 2 cut(s) 110, 786
BmrFI CCNGG 3 cut(s) 211, 230, 946
BpiI GAAGAC 1 cut(s) 663
BpmI CTGGAG 1 cut(s) 568
Bpu14I TTCGAA 2 cut(s) 258, 704
BpuEI CTTGAG 2 cut(s) 344, 874
BpuMI CCSGG 3 cut(s) 211, 230, 946
BsaHI GRCGYC 3 cut(s) 458, 521, 609
BsaJI CCNNGG 4 cut(s) 243, 453, 505, 1029
BsaWI WCCGGW 5 cut(s) 71, 468, 756, 787, 1199
BsaXI ACNNNNNCTCC 6 cut(s) 410, 440, 566, 572, 596, 602
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 210, 468, 511
Bse118I RCCGGY 1 cut(s) 756
Bse1I ACTGG 2 cut(s) 54, 383
BseDI CCNNGG 4 cut(s) 243, 453, 505, 1029
BseGI GGATG 5 cut(s) 342, 369, 449, 807, 1104
BseLI CCNNNNNNNGG 4 cut(s) 209, 210, 468, 511
BseMII CTCAG 2 cut(s) 656, 968
BseNI ACTGG 2 cut(s) 54, 383
BseRI GAGGAG 6 cut(s) 530, 1108, 1145, 1148, 1151, 1169
BseX3I CGGCCG 1 cut(s) 1026
BseXI GCAGC 2 cut(s) 615, 638
Bsh1236I CGCG 4 cut(s) 264, 545, 614, 664
Bsh1285I CGRYCG 3 cut(s) 552, 950, 1029
BshFI GGCC 3 cut(s) 465, 568, 1028
BshTI ACCGGT 1 cut(s) 756
BsiEI CGRYCG 3 cut(s) 552, 950, 1029
BsiHKCI CYCGRG 2 cut(s) 244, 1168
BslI CCNNNNNNNGG 4 cut(s) 209, 210, 468, 511
BsmAI GTCTC 2 cut(s) 481, 512
BsmBI CGTCTC 1 cut(s) 512
BsnI GGCC 3 cut(s) 465, 568, 1028
BsoBI CYCGRG 2 cut(s) 244, 1168
Bsp119I TTCGAA 2 cut(s) 258, 704
Bsp1286I GDGCHC 1 cut(s) 113
Bsp143I GATC 3 cut(s) 328, 501, 774
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 9 cut(s) 429, 545, 612, 623, 626, 866, 874, 878, 1025
BspANI GGCC 3 cut(s) 465, 568, 1028
BspCNI CTCAG 2 cut(s) 655, 967
BspFNI CGCG 4 cut(s) 264, 545, 614, 664
BspLI GGNNCC 2 cut(s) 110, 786
BspPI GGATC 1 cut(s) 496
BspT104I TTCGAA 2 cut(s) 258, 704
BsrFI RCCGGY 1 cut(s) 756
BsrI ACTGG 2 cut(s) 54, 383
BssAI RCCGGY 1 cut(s) 756
BssECI CCNNGG 4 cut(s) 243, 453, 505, 1029
BssMI GATC 3 cut(s) 328, 501, 774
BssNI GRCGYC 3 cut(s) 458, 521, 609
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 308
Bst4CI ACNGT 1 cut(s) 17
Bst6I CTCTTC 1 cut(s) 1055
BstACI GRCGYC 3 cut(s) 458, 521, 609
BstBI TTCGAA 2 cut(s) 258, 704
BstC8I GCNNGC 2 cut(s) 876, 1017
BstDEI CTNAG 2 cut(s) 642, 954
BstDSI CCRYGG 1 cut(s) 453
BstF5I GGATG 5 cut(s) 342, 369, 449, 807, 1104
BstFNI CGCG 4 cut(s) 264, 545, 614, 664
BstKTI GATC 3 cut(s) 331, 504, 777
BstMAI GTCTC 2 cut(s) 481, 512
BstMBI GATC 3 cut(s) 328, 501, 774
BstMCI CGRYCG 3 cut(s) 552, 950, 1029
BstMWI GCNNNNNNNGC 3 cut(s) 172, 1025, 1031
BstSCI CCNGG 3 cut(s) 209, 228, 944
BstUI CGCG 4 cut(s) 264, 545, 614, 664
BstV1I GCAGC 2 cut(s) 615, 638
BstV2I GAAGAC 1 cut(s) 663
BstZI CGGCCG 1 cut(s) 1026
BsuRI GGCC 3 cut(s) 465, 568, 1028
BtgI CCRYGG 1 cut(s) 453
BtgZI GCGATG 1 cut(s) 896
BtrI CACGTC 1 cut(s) 529
BtsCI GGATG 5 cut(s) 342, 369, 449, 807, 1104
BtsI GCAGTG 1 cut(s) 1041
BtsIMutI CAGTG 1 cut(s) 1041
Cac8I GCNNGC 2 cut(s) 876, 1017
Cfr10I RCCGGY 1 cut(s) 756
Cfr13I GGNCC 2 cut(s) 141, 759
CseI GACGC 5 cut(s) 253, 259, 447, 529, 617
Csp6I GTAC 1 cut(s) 447
CspAI ACCGGT 1 cut(s) 756
CviAII CATG 2 cut(s) 454, 820
CviQI GTAC 1 cut(s) 447
DdeI CTNAG 2 cut(s) 642, 954
DpnI GATC 3 cut(s) 330, 503, 776
DpnII GATC 3 cut(s) 328, 501, 774
DraI TTTAAA 1 cut(s) 10
DraIII CACNNNGTG 1 cut(s) 846
DrdI GACNNNNNNGTC 1 cut(s) 599
DseDI GACNNNNNNGTC 1 cut(s) 599
EaeI YGGCCR 2 cut(s) 463, 1026
EagI CGGCCG 1 cut(s) 1026
Eam1104I CTCTTC 1 cut(s) 1055
EarI CTCTTC 1 cut(s) 1055
EciI GGCGGA 1 cut(s) 418
EclXI CGGCCG 1 cut(s) 1026
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 113
Eco47I GGWCC 2 cut(s) 141, 759
Eco52I CGGCCG 1 cut(s) 1026
Eco57I CTGAAG 1 cut(s) 1178
Eco88I CYCGRG 2 cut(s) 244, 1168
EcoO109I RGGNCCY 1 cut(s) 141
EcoRI GAATTC 1 cut(s) 700
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 113
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 512
FaeI CATG 2 cut(s) 457, 823
FaiI YATR 6 cut(s) 236, 455, 723, 741, 821, 981
FatI CATG 2 cut(s) 453, 819
FauI CCCGC 2 cut(s) 630, 867
FblI GTMKAC 1 cut(s) 950
Fnu4HI GCNGC 7 cut(s) 546, 612, 626, 629, 652, 879, 1026
FokI GGATG 5 cut(s) 329, 356, 436, 814, 1111
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 7 cut(s) 546, 612, 626, 629, 652, 879, 1026
FspBI CTAG 2 cut(s) 570, 1188
GluI GCNGC 7 cut(s) 546, 612, 626, 629, 652, 879, 1026
GsuI CTGGAG 1 cut(s) 568
HaeIII GGCC 3 cut(s) 465, 568, 1028
HgaI GACGC 5 cut(s) 253, 259, 447, 529, 617
Hin1I GRCGYC 3 cut(s) 458, 521, 609
Hin1II CATG 2 cut(s) 457, 823
HincII GTYRAC 3 cut(s) 558, 618, 951
HindII GTYRAC 3 cut(s) 558, 618, 951
HphI GGTGA 3 cut(s) 289, 325, 817
Hpy166II GTNNAC 7 cut(s) 37, 64, 313, 366, 558, 618, 951
Hpy188I TCNGA 7 cut(s) 21, 108, 487, 506, 592, 682, 1240
Hpy188III TCNNGA 3 cut(s) 637, 748, 956
Hpy8I GTNNAC 7 cut(s) 37, 64, 313, 366, 558, 618, 951
Hpy99I CGWCG 5 cut(s) 567, 596, 611, 664, 700
HpyAV CCTTC 5 cut(s) 177, 265, 1015, 1081, 1120
HpyCH4III ACNGT 1 cut(s) 17
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 4 cut(s) 152, 282, 849, 1211
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 1025, 1031
HpyF3I CTNAG 2 cut(s) 642, 954
HpySE526I ACGT 1 cut(s) 528
Hsp92I GRCGYC 3 cut(s) 458, 521, 609
Hsp92II CATG 2 cut(s) 457, 823
Kzo9I GATC 3 cut(s) 328, 501, 774
LmnI GCTCC 2 cut(s) 108, 593
Lsp1109I GCAGC 2 cut(s) 615, 638
MaeI CTAG 2 cut(s) 570, 1188
MaeII ACGT 1 cut(s) 528
MaeIII GTNAC 4 cut(s) 248, 572, 752, 815
MalI GATC 3 cut(s) 330, 503, 776
MboI GATC 3 cut(s) 328, 501, 774
MboII GAAGA 2 cut(s) 663, 1072
MhlI GDGCHC 1 cut(s) 113
MluCI AATT 4 cut(s) 153, 388, 700, 1231
MluI ACGCGT 1 cut(s) 262
MlyI GAGTC 9 cut(s) 308, 413, 503, 553, 605, 634, 946, 997, 1160
MmeI TCCRAC 3 cut(s) 615, 867, 1083
MseI TTAA 6 cut(s) 9, 83, 218, 771, 1008, 1230
MspA1I CMGCKG 1 cut(s) 628
MspR9I CCNGG 3 cut(s) 211, 230, 946
MvnI CGCG 4 cut(s) 264, 545, 614, 664
MwoI GCNNNNNNNGC 3 cut(s) 172, 1025, 1031
NciI CCSGG 3 cut(s) 211, 230, 946
NcoI CCATGG 1 cut(s) 453
NdeII GATC 3 cut(s) 328, 501, 774
NlaIII CATG 2 cut(s) 457, 823
NlaIV GGNNCC 2 cut(s) 110, 786
NmeAIII GCCGAG 2 cut(s) 672, 1054
NmuCI GTSAC 3 cut(s) 248, 572, 815
NspV TTCGAA 2 cut(s) 258, 704
PcsI WCGNNNNNNNCGW 3 cut(s) 549, 644, 665
PfeI GAWTC 2 cut(s) 890, 941
PinAI ACCGGT 1 cut(s) 756
PkrI GCNGC 7 cut(s) 547, 613, 627, 630, 653, 880, 1027
PleI GAGTC 9 cut(s) 308, 413, 502, 553, 604, 634, 946, 996, 1160
PpsI GAGTC 9 cut(s) 308, 413, 502, 553, 604, 634, 946, 996, 1160
PpuMI RGGWCCY 1 cut(s) 141
PshBI ATTAAT 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 141
PspN4I GGNNCC 2 cut(s) 110, 786
PspPI GGNCC 2 cut(s) 141, 759
PspPPI RGGWCCY 1 cut(s) 141
RsaI GTAC 1 cut(s) 448
RsaNI GTAC 1 cut(s) 447
SalI GTCGAC 1 cut(s) 949
SaqAI TTAA 6 cut(s) 9, 83, 218, 771, 1008, 1230
SatI GCNGC 7 cut(s) 546, 612, 626, 629, 652, 879, 1026
Sau3AI GATC 3 cut(s) 328, 501, 774
Sau96I GGNCC 2 cut(s) 141, 759
SchI GAGTC 9 cut(s) 308, 413, 503, 553, 605, 634, 946, 997, 1160
ScrFI CCNGG 3 cut(s) 211, 230, 946
SduI GDGCHC 1 cut(s) 113
SfuI TTCGAA 2 cut(s) 258, 704
SinI GGWCC 2 cut(s) 141, 759
SmlI CTYRAG 2 cut(s) 359, 853
SmoI CTYRAG 2 cut(s) 359, 853
Sse9I AATT 4 cut(s) 153, 388, 700, 1231
SsiI CCGC 9 cut(s) 429, 545, 612, 623, 626, 866, 874, 878, 1025
SspMI CTAG 2 cut(s) 570, 1188
StyD4I CCNGG 3 cut(s) 209, 228, 944
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 1 cut(s) 17
TaiI ACGT 1 cut(s) 531
TaqI TCGA 8 cut(s) 258, 395, 417, 638, 687, 704, 950, 1183
TaqII GACCGA 2 cut(s) 566, 589
TasI AATT 4 cut(s) 153, 388, 700, 1231
TauI GCSGC 5 cut(s) 548, 614, 628, 881, 1028
TfiI GAWTC 2 cut(s) 890, 941
Tru1I TTAA 6 cut(s) 9, 83, 218, 771, 1008, 1230
Tru9I TTAA 6 cut(s) 9, 83, 218, 771, 1008, 1230
TscAI CASTG 1 cut(s) 1041
TseFI GTSAC 3 cut(s) 248, 572, 815
TseI GCWGC 2 cut(s) 628, 651
Tsp45I GTSAC 3 cut(s) 248, 572, 815
TspDTI ATGAA 1 cut(s) 184
TspGWI ACGGA 2 cut(s) 328, 434
TspRI CASTG 1 cut(s) 1041
VpaK11BI GGWCC 2 cut(s) 141, 759
VspI ATTAAT 1 cut(s) 218
XapI RAATTY 1 cut(s) 700
XcmI CCANNNNNNNNNTGG 1 cut(s) 120
XmiI GTMKAC 1 cut(s) 950
XspI CTAG 2 cut(s) 570, 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.