pycom17g03600

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012888
Physical Location & Seq
Reverse (-)
2412060 .. 2413733
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g03600.1

Sequence Viewer

Length: 1674 bp
ATGAAGCTGATGGTCCACAAATCAAAAGAGAACGAATGGGCTTTCGCGACGCCGGAGAGAGCTGCGGAGATGATCAGCAGTGCAAGCACGAGTCCCCGGCGGAGATGGAAGCTTTTCCAGAGGTCGTCGTCGGCAATTCCGTCGAAAACTTCAAAGCCGCAAGCTCCCAAAGAGTTCGTCTGTCCAATCTCCGGTTCTCTCATGGCGGACCCGGTCATCGTTTCGTCCGGCCACACCTTCGAGCGCGCCTGCGTCCAAGCCTGCAAGTCCCTCAGCTTCACGCCTGCTCTCCCGGACTCTCCGCCGCCAGATTTCTCCTCCGTAATCTCCAACCTCGCCCTCAGGTCCGCCATTCTCAGCTGGTGCCACAAGTCCTTCGTCGATCCTCCCAAGCCCCTCGATTTCGCCTCCGCCGAGAAGATCGTCCGCGCCTCCATTGGTTCCCGGAACGAAACCAAGCCCCAGAACGTCGTAGTTTCGGATAATCAGCTGTTGATCAACGGTGTTAAGGACACCCCTAGAGTGAATTTCACTCTCGCGGCCACCGAGCTGACTCGGCGCCCAACTCACTTCCCCTCGAGCTCCGACGAATCGGTCACCGCGGCTCCGCCTCTCCCGTTCTCAACTCCACCGAGCTGCTACTCTTCCCCGTCGTCTTCATCCTCGGAAATTGAAACTTTAAATCCTAATTGCAACAGCAATCCCTCCCTCTCTGTAGAAGACGAAATCTCGATCAAGCTCAGAAGCTGCCACGTGTTCGAAATCGAGGAGGCCCTGGCCTCACTCAGGAAGATCACGCGGACGAGAGAGGACGCCCGGGCCCGTCTCTGCATCCCGCGGTTGCTCTCCGCCCTCCGCCCTCTGATTATATCCAGGTACACCGCCGTTCAGGTGGACTCCGTCGCAGCCTTGGTCAATCTGTCGCTGGAAAAGTCAAACAAGATTAAGATCGTACGGTCAGGAGTCCTCCCTCCTCTGATCGACGTGCTCAAGGCCGGAACCCCTGAGGCGCAGGAACACGCTTCCAGTGCGCTCTTCAGCTTGGCACTTGACGACGACTGCAAGACCGCCATTGGCGTTTTGGGCGCGTTACCGCCATTGATGCACTTACTCCGGTCCCAGAGCGAGCGGACTCGTCATGACTCGGCACTCGCTTTGTTCCACCTCTCGCTCGTTCAGAGTAACCGGTCCAAGTTGGTCAAGCTCGGCTCGGTTCCGGTTCTTTTGAGAATGGTGAAGTCGGGTCACATGACGGGTCGGGTATTGCTCACATTGTGTAATTTGAGTTTGTGTGCCGACGGGCGGGCCGCATTGTTGGATTCGGGTGGGGTGGAGTGCTTGGTGGGTGTGTTAAGGGGAAACGAGTTTGACTCGAAGGCGACACAAGAGAGCTGTGTGGCCACATTGTATGGGTTGAGCTACGGCGGTTTGAGGTTCATAGGGCTGGCGAAGACGGCGGGCTTGGTGGAGGTGTTGAGAGAGGTGGAGAAAAAGGGAAGCGAACGGGCGAGGGAGAAGGCGAGGCGGATGTCGGAGATGATGAGAGGGAAGGAGAAGGAAGAAGAAGATGAAGTGGATTGGGAGGAAGTGCTCAACTCGGGGTTTGGGAACCGAACTCGGTGTCAACCCGGTGGTGAACTGGAGGGGTCGAGTGTCAACTCATTGGACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

558

Amino Acids

60.53

Weight (kDa)

8.67

Isoelectric Point (pI)

55.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 55 - 123 4.9e-10 U-box domain
ARM_PUB PF25598 256 - 448 1.4e-08 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 296 - 492 3.7e-10 ARMC5
Arm PF00514 351 - 390 1.4e-06 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40140
fragaria_vesca FvH4_6g50090
malus_domestica MD09G1039200.v1.1 MD17G1040200.v1.1
prunus_persica Prupe.3G278800_v2.0.a1
pyrus_communis pycom111g03090 pycom17g03600
rosa_chinensis RchiOBHm_Chr2g0170361
rosa_laevigata RLG00000021949
rosa_multiflora Rmu_co8477427.1_g000001 Rmu_sc0011601.1_g000012
rosa_roxburghii Rroxscaffold_2G00081400
rosa_rugosa Rorug02G0548500
rosa_samantha Rh2AG619900 Rh2BG630900 Rh2CG600900 Rh2DG643800
rosa_wichuraiana Rw2G051360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 593
AccB1I GGYRCC 2 cut(s) 363, 558
AccBSI CCGCTC 1 cut(s) 1129
AccII CGCG 8 cut(s) 47, 246, 429, 539, 602, 799, 838, 1088
AclWI GGATC 1 cut(s) 377
AcoI YGGCCR 3 cut(s) 229, 540, 1398
AcsI RAATTY 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 1021
AcvI CACGTG 1 cut(s) 754
AcyI GRCGYC 3 cut(s) 50, 559, 813
AdeI CACNNNGTG 1 cut(s) 1275
AfaI GTAC 2 cut(s) 878, 954
AfiI CCNNNNNNNGG 3 cut(s) 191, 786, 1302
AflIII ACRYGT 1 cut(s) 753
AgeI ACCGGT 1 cut(s) 1185
AgsI TTSAA 2 cut(s) 153, 674
AjiI CACGTC 1 cut(s) 985
AjnI CCWGG 2 cut(s) 774, 872
AjuI GAANNNNNNNTTGG 2 cut(s) 178, 210
Alw21I GWGCWC 3 cut(s) 584, 990, 1593
Alw26I GTCTC 1 cut(s) 830
AlwI GGATC 1 cut(s) 377
AlwNI CAGNNNCTG 1 cut(s) 747
Ama87I CYCGRG 3 cut(s) 577, 816, 1597
AoxI GGCC 8 cut(s) 229, 540, 771, 777, 819, 993, 1305, 1398
ApaI GGGCCC 1 cut(s) 823
ApeKI GCWGC 4 cut(s) 62, 636, 747, 905
ApoI RAATTY 1 cut(s) 526
ArsI GACNNNNNNTTYG 4 cut(s) 162, 194, 1606, 1638
AsiGI ACCGGT 1 cut(s) 1185
Asp700I GAANNNNTTC 1 cut(s) 113
AspLEI GCGC 7 cut(s) 246, 248, 431, 561, 1012, 1033, 1088
AspS9I GGNCC 9 cut(s) 13, 208, 345, 772, 819, 820, 1116, 1188, 1305
AsuC2I CCSGG 7 cut(s) 97, 212, 293, 445, 817, 818, 1629
AsuHPI GGTGA 3 cut(s) 589, 1246, 1646
AsuII TTCGAA 1 cut(s) 759
AvaI CYCGRG 3 cut(s) 577, 816, 1597
AvaII GGWCC 5 cut(s) 13, 208, 345, 1116, 1188
AxyI CCTNAGG 2 cut(s) 341, 1005
BaeGI GKGCMC 1 cut(s) 823
BalI TGGCCA 1 cut(s) 1400
BanI GGYRCC 2 cut(s) 363, 558
BanII GRGCYC 2 cut(s) 584, 823
BauI CACGAG 1 cut(s) 88
BbrPI CACGTG 1 cut(s) 754
BbsI GAAGAC 3 cut(s) 648, 726, 1457
Bbv12I GWGCWC 3 cut(s) 584, 990, 1593
BbvCI CCTCAGC 1 cut(s) 272
BbvI GCAGC 4 cut(s) 49, 623, 734, 917
BccI CCATC 2 cut(s) 4, 99
BceAI ACGGC 3 cut(s) 869, 1438, 1470
BcgI CGANNNNNNTGC 2 cut(s) 123, 157
BciT130I CCWGG 2 cut(s) 776, 874
BclI TGATCA 2 cut(s) 72, 495
BcnI CCSGG 7 cut(s) 97, 212, 293, 445, 817, 818, 1629
BcoDI GTCTC 1 cut(s) 830
BfaI CTAG 1 cut(s) 519
BfmI CTRYAG 1 cut(s) 714
BfoI RGCGCY 1 cut(s) 562
BisI GCNGC 9 cut(s) 63, 158, 305, 540, 603, 637, 748, 906, 1308
BlsI GCNGC 9 cut(s) 64, 159, 306, 541, 604, 638, 749, 907, 1309
Bme1390I CCNGG 9 cut(s) 97, 212, 293, 445, 776, 817, 818, 874, 1629
Bme18I GGWCC 5 cut(s) 13, 208, 345, 1116, 1188
BmeT110I CYCGRG 3 cut(s) 577, 816, 1597
BmgBI CACGTC 1 cut(s) 985
BmgT120I GGNCC 9 cut(s) 13, 208, 345, 772, 819, 820, 1116, 1188, 1305
BmrFI CCNGG 9 cut(s) 97, 212, 293, 445, 776, 817, 818, 874, 1629
BmsI GCATC 2 cut(s) 840, 1092
BpiI GAAGAC 3 cut(s) 648, 726, 1457
BplI GAGNNNNNCTC 2 cut(s) 1355, 1387
BpmI CTGGAG 1 cut(s) 1661
Bpu10I CCTNAGC 1 cut(s) 272
Bpu14I TTCGAA 1 cut(s) 759
BpuEI CTTGAG 1 cut(s) 974
BpuMI CCSGG 7 cut(s) 97, 212, 293, 445, 817, 818, 1629
BsaAI YACGTR 1 cut(s) 754
BsaBI GATNNNNATC 1 cut(s) 947
BsaHI GRCGYC 3 cut(s) 50, 559, 813
BsaJI CCNNGG 7 cut(s) 95, 600, 663, 774, 816, 836, 909
BsaWI WCCGGW 4 cut(s) 191, 1113, 1185, 1216
BsaXI ACNNNNNCTCC 2 cut(s) 589, 619
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 786, 1302
Bse118I RCCGGY 1 cut(s) 1185
Bse1I ACTGG 2 cut(s) 1026, 1644
Bse21I CCTNAGG 2 cut(s) 341, 1005
Bse8I GATNNNNATC 1 cut(s) 947
BseBI CCWGG 2 cut(s) 776, 874
BseDI CCNNGG 7 cut(s) 95, 600, 663, 774, 816, 836, 909
BseGI GGATG 3 cut(s) 659, 831, 1533
BseJI GATNNNNATC 1 cut(s) 947
BseLI CCNNNNNNNGG 3 cut(s) 191, 786, 1302
BseMII CTCAG 6 cut(s) 286, 355, 370, 754, 799, 996
BseNI ACTGG 2 cut(s) 1026, 1644
BsePI GCGCGC 1 cut(s) 244
BseRI GAGGAG 3 cut(s) 307, 782, 963
BseSI GKGCMC 1 cut(s) 823
BseXI GCAGC 4 cut(s) 49, 623, 734, 917
Bsh1236I CGCG 8 cut(s) 47, 246, 429, 539, 602, 799, 838, 1088
BshFI GGCC 8 cut(s) 231, 542, 773, 779, 821, 995, 1307, 1400
BshNI GGYRCC 2 cut(s) 363, 558
BshTI ACCGGT 1 cut(s) 1185
BsiHKAI GWGCWC 3 cut(s) 584, 990, 1593
BsiHKCI CYCGRG 3 cut(s) 577, 816, 1597
BsiWI CGTACG 1 cut(s) 952
BslFI GGGAC 3 cut(s) 78, 253, 1102
BslI CCNNNNNNNGG 3 cut(s) 191, 786, 1302
BsmAI GTCTC 1 cut(s) 830
BsmBI CGTCTC 1 cut(s) 830
BsmFI GGGAC 3 cut(s) 78, 253, 1102
BsnI GGCC 8 cut(s) 231, 542, 773, 779, 821, 995, 1307, 1400
BsoBI CYCGRG 3 cut(s) 577, 816, 1597
Bsp119I TTCGAA 1 cut(s) 759
Bsp120I GGGCCC 1 cut(s) 819
Bsp1286I GDGCHC 4 cut(s) 584, 823, 990, 1593
Bsp143I GATC 8 cut(s) 72, 382, 420, 495, 732, 792, 948, 978
Bsp68I TCGCGA 1 cut(s) 47
BspANI GGCC 8 cut(s) 231, 542, 773, 779, 821, 995, 1307, 1400
BspCNI CTCAG 6 cut(s) 285, 354, 369, 753, 798, 997
BspFNI CGCG 8 cut(s) 47, 246, 429, 539, 602, 799, 838, 1088
BspHI TCATGA 1 cut(s) 1138
BspPI GGATC 1 cut(s) 377
BspQI GCTCTTC 1 cut(s) 1040
BspT104I TTCGAA 1 cut(s) 759
BspT107I GGYRCC 2 cut(s) 363, 558
BsrBI CCGCTC 1 cut(s) 1129
BsrFI RCCGGY 1 cut(s) 1185
BsrI ACTGG 2 cut(s) 1026, 1644
BssAI RCCGGY 1 cut(s) 1185
BssECI CCNNGG 7 cut(s) 95, 600, 663, 774, 816, 836, 909
BssHII GCGCGC 1 cut(s) 244
BssMI GATC 8 cut(s) 72, 382, 420, 495, 732, 792, 948, 978
BssNI GRCGYC 3 cut(s) 50, 559, 813
BssSI CACGAG 1 cut(s) 88
BssT1I CCWWGG 1 cut(s) 909
Bst2BI CACGAG 1 cut(s) 88
Bst2UI CCWGG 2 cut(s) 776, 874
Bst4CI ACNGT 2 cut(s) 503, 957
Bst6I CTCTTC 2 cut(s) 649, 1040
BstACI GRCGYC 3 cut(s) 50, 559, 813
BstBAI YACGTR 1 cut(s) 754
BstBI TTCGAA 1 cut(s) 759
BstDEI CTNAG 6 cut(s) 272, 341, 356, 740, 785, 1005
BstDSI CCRYGG 2 cut(s) 600, 836
BstEII GGTNACC 1 cut(s) 595
BstENI CCTNNNNNAGG 1 cut(s) 784
BstF5I GGATG 3 cut(s) 659, 831, 1533
BstFNI CGCG 8 cut(s) 47, 246, 429, 539, 602, 799, 838, 1088
BstH2I RGCGCY 1 cut(s) 562
BstHHI GCGC 7 cut(s) 246, 248, 431, 561, 1012, 1033, 1088
BstKTI GATC 8 cut(s) 75, 385, 423, 498, 735, 795, 951, 981
BstMAI GTCTC 1 cut(s) 830
BstMBI GATC 8 cut(s) 72, 382, 420, 495, 732, 792, 948, 978
BstMWI GCNNNNNNNGC 6 cut(s) 84, 556, 1028, 1083, 1102, 1454
BstNI CCWGG 2 cut(s) 776, 874
BstPI GGTNACC 1 cut(s) 595
BstSCI CCNGG 9 cut(s) 95, 210, 291, 443, 774, 815, 816, 872, 1627
BstSFI CTRYAG 1 cut(s) 714
BstSLI GKGCMC 1 cut(s) 823
BstUI CGCG 8 cut(s) 47, 246, 429, 539, 602, 799, 838, 1088
BstV1I GCAGC 4 cut(s) 49, 623, 734, 917
BstV2I GAAGAC 3 cut(s) 648, 726, 1457
Bsu36I CCTNAGG 2 cut(s) 341, 1005
BsuRI GGCC 8 cut(s) 231, 542, 773, 779, 821, 995, 1307, 1400
BtgI CCRYGG 2 cut(s) 600, 836
BtrI CACGTC 1 cut(s) 985
BtsCI GGATG 3 cut(s) 659, 831, 1533
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 2 cut(s) 85, 1033
BtuMI TCGCGA 1 cut(s) 47
CaiI CAGNNNCTG 1 cut(s) 747
CciI TCATGA 1 cut(s) 1138
CfoI GCGC 7 cut(s) 246, 248, 431, 561, 1012, 1033, 1088
Cfr10I RCCGGY 1 cut(s) 1185
Cfr13I GGNCC 9 cut(s) 13, 208, 345, 772, 819, 820, 1116, 1188, 1305
Cfr42I CCGCGG 2 cut(s) 603, 839
Cfr9I CCCGGG 1 cut(s) 816
CseI GACGC 3 cut(s) 58, 241, 821
Csp6I GTAC 2 cut(s) 877, 953
CspAI ACCGGT 1 cut(s) 1185
CviAII CATG 3 cut(s) 202, 1139, 1249
CviQI GTAC 2 cut(s) 877, 953
DdeI CTNAG 6 cut(s) 272, 341, 356, 740, 785, 1005
DinI GGCGCC 1 cut(s) 560
DpnI GATC 8 cut(s) 74, 384, 422, 497, 734, 794, 950, 980
DpnII GATC 8 cut(s) 72, 382, 420, 495, 732, 792, 948, 978
DraI TTTAAA 1 cut(s) 681
DraIII CACNNNGTG 1 cut(s) 1275
DrdI GACNNNNNNGTC 1 cut(s) 593
DseDI GACNNNNNNGTC 1 cut(s) 593
EaeI YGGCCR 3 cut(s) 229, 540, 1398
Eam1104I CTCTTC 2 cut(s) 649, 1040
EarI CTCTTC 2 cut(s) 649, 1040
EciI GGCGGA 9 cut(s) 115, 221, 291, 337, 400, 597, 838, 845, 1540
Ecl136II GAGCTC 1 cut(s) 582
Eco130I CCWWGG 1 cut(s) 909
Eco24I GRGCYC 2 cut(s) 584, 823
Eco47I GGWCC 5 cut(s) 13, 208, 345, 1116, 1188
Eco53kI GAGCTC 1 cut(s) 582
Eco57I CTGAAG 1 cut(s) 1021
Eco72I CACGTG 1 cut(s) 754
Eco81I CCTNAGG 2 cut(s) 341, 1005
Eco88I CYCGRG 3 cut(s) 577, 816, 1597
Eco91I GGTNACC 1 cut(s) 595
EcoICRI GAGCTC 1 cut(s) 582
EcoNI CCTNNNNNAGG 1 cut(s) 784
EcoO109I RGGNCCY 1 cut(s) 772
EcoO65I GGTNACC 1 cut(s) 595
EcoRII CCWGG 2 cut(s) 774, 872
EcoT14I CCWWGG 1 cut(s) 909
EcoT38I GRGCYC 2 cut(s) 584, 823
EgeI GGCGCC 1 cut(s) 560
EheI GGCGCC 1 cut(s) 560
ErhI CCWWGG 1 cut(s) 909
Esp3I CGTCTC 1 cut(s) 830
FaeI CATG 3 cut(s) 205, 1142, 1252
FaiI YATR 6 cut(s) 203, 869, 1140, 1250, 1410, 1439
FaqI GGGAC 3 cut(s) 78, 253, 1102
FatI CATG 3 cut(s) 201, 1138, 1248
FauI CCCGC 3 cut(s) 843, 1296, 1450
FbaI TGATCA 2 cut(s) 72, 495
Fnu4HI GCNGC 9 cut(s) 63, 158, 305, 540, 603, 637, 748, 906, 1308
FokI GGATG 3 cut(s) 646, 818, 1540
FriOI GRGCYC 2 cut(s) 584, 823
Fsp4HI GCNGC 9 cut(s) 63, 158, 305, 540, 603, 637, 748, 906, 1308
FspBI CTAG 1 cut(s) 519
GlaI GCGC 7 cut(s) 245, 247, 430, 560, 1011, 1032, 1087
GluI GCNGC 9 cut(s) 63, 158, 305, 540, 603, 637, 748, 906, 1308
GsuI CTGGAG 1 cut(s) 1661
HaeII RGCGCY 1 cut(s) 562
HaeIII GGCC 8 cut(s) 231, 542, 773, 779, 821, 995, 1307, 1400
HgaI GACGC 3 cut(s) 58, 241, 821
HhaI GCGC 7 cut(s) 246, 248, 431, 561, 1012, 1033, 1088
Hin1I GRCGYC 3 cut(s) 50, 559, 813
Hin1II CATG 3 cut(s) 205, 1142, 1252
Hin6I GCGC 7 cut(s) 244, 246, 429, 559, 1010, 1031, 1086
HinP1I GCGC 7 cut(s) 244, 246, 429, 559, 1010, 1031, 1086
HincII GTYRAC 2 cut(s) 1625, 1657
HindII GTYRAC 2 cut(s) 1625, 1657
HindIII AAGCTT 1 cut(s) 110
HphI GGTGA 3 cut(s) 589, 1246, 1646
Hpy166II GTNNAC 6 cut(s) 16, 879, 895, 1625, 1637, 1657
Hpy188I TCNGA 8 cut(s) 481, 586, 667, 743, 864, 978, 1179, 1534
Hpy188III TCNNGA 6 cut(s) 46, 118, 730, 787, 960, 1139
Hpy8I GTNNAC 6 cut(s) 16, 879, 895, 1625, 1637, 1657
HpyAV CCTTC 6 cut(s) 247, 385, 1369, 1510, 1543, 1549
HpyCH4III ACNGT 2 cut(s) 503, 957
HpyCH4IV ACGT 3 cut(s) 468, 753, 984
HpyCH4V TGCA 6 cut(s) 83, 264, 693, 831, 1062, 1105
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 556, 1028, 1083, 1102, 1454
HpyF3I CTNAG 6 cut(s) 272, 341, 356, 740, 785, 1005
HpySE526I ACGT 3 cut(s) 468, 753, 984
Hsp92I GRCGYC 3 cut(s) 50, 559, 813
Hsp92II CATG 3 cut(s) 205, 1142, 1252
HspAI GCGC 7 cut(s) 244, 246, 429, 559, 1010, 1031, 1086
KasI GGCGCC 1 cut(s) 558
Ksp22I TGATCA 2 cut(s) 72, 495
KspI CCGCGG 2 cut(s) 603, 839
Kzo9I GATC 8 cut(s) 72, 382, 420, 495, 732, 792, 948, 978
LguI GCTCTTC 1 cut(s) 1040
LmnI GCTCC 3 cut(s) 169, 587, 610
Lsp1109I GCAGC 4 cut(s) 49, 623, 734, 917
LweI GCATC 2 cut(s) 840, 1092
MaeI CTAG 1 cut(s) 519
MaeII ACGT 3 cut(s) 468, 753, 984
MaeIII GTNAC 4 cut(s) 595, 1089, 1181, 1244
MalI GATC 8 cut(s) 74, 384, 422, 497, 734, 794, 950, 980
MbiI CCGCTC 1 cut(s) 1129
MboI GATC 8 cut(s) 72, 382, 420, 495, 732, 792, 948, 978
MhlI GDGCHC 4 cut(s) 584, 823, 990, 1593
MlsI TGGCCA 1 cut(s) 1400
MluCI AATT 5 cut(s) 135, 526, 669, 688, 1279
MluNI TGGCCA 1 cut(s) 1400
Mly113I GGCGCC 1 cut(s) 559
MlyI GAGTC 8 cut(s) 100, 290, 547, 890, 972, 1126, 1136, 1364
MmeI TCCRAC 4 cut(s) 354, 609, 1296, 1512
Mox20I TGGCCA 1 cut(s) 1400
MroXI GAANNNNTTC 1 cut(s) 113
MscI TGGCCA 1 cut(s) 1400
MseI TTAA 4 cut(s) 507, 680, 945, 1352
Msp20I TGGCCA 1 cut(s) 1400
MspA1I CMGCKG 4 cut(s) 360, 490, 602, 838
MspR9I CCNGG 9 cut(s) 97, 212, 293, 445, 776, 817, 818, 874, 1629
MvaI CCWGG 2 cut(s) 776, 874
MvnI CGCG 8 cut(s) 47, 246, 429, 539, 602, 799, 838, 1088
MwoI GCNNNNNNNGC 6 cut(s) 84, 556, 1028, 1083, 1102, 1454
NarI GGCGCC 1 cut(s) 559
NciI CCSGG 7 cut(s) 97, 212, 293, 445, 817, 818, 1629
NdeII GATC 8 cut(s) 72, 382, 420, 495, 732, 792, 948, 978
NlaIII CATG 3 cut(s) 205, 1142, 1252
NmeAIII GCCGAG 4 cut(s) 439, 535, 1124, 1185
NmuCI GTSAC 2 cut(s) 595, 1244
NruI TCGCGA 1 cut(s) 47
NspV TTCGAA 1 cut(s) 759
PaeR7I CTCGAG 1 cut(s) 577
PagI TCATGA 1 cut(s) 1138
PauI GCGCGC 1 cut(s) 244
PciSI GCTCTTC 1 cut(s) 1040
PcsI WCGNNNNNNNCGW 3 cut(s) 137, 411, 543
PdmI GAANNNNTTC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 590, 1319
Pfl23II CGTACG 1 cut(s) 952
PflFI GACNNNGTC 2 cut(s) 212, 899
PfoI TCCNGGA 2 cut(s) 291, 443
PinAI ACCGGT 1 cut(s) 1185
PkrI GCNGC 9 cut(s) 64, 159, 306, 541, 604, 638, 749, 907, 1309
PleI GAGTC 8 cut(s) 99, 290, 547, 890, 971, 1126, 1136, 1364
PluTI GGCGCC 1 cut(s) 562
PmaCI CACGTG 1 cut(s) 754
PmlI CACGTG 1 cut(s) 754
PpsI GAGTC 8 cut(s) 99, 290, 547, 890, 971, 1126, 1136, 1364
Ppu21I YACGTR 1 cut(s) 754
Psp124BI GAGCTC 1 cut(s) 584
Psp6I CCWGG 2 cut(s) 774, 872
PspCI CACGTG 1 cut(s) 754
PspEI GGTNACC 1 cut(s) 595
PspGI CCWGG 2 cut(s) 774, 872
PspLI CGTACG 1 cut(s) 952
PspOMI GGGCCC 1 cut(s) 819
PspPI GGNCC 9 cut(s) 13, 208, 345, 772, 819, 820, 1116, 1188, 1305
PspXI VCTCGAGB 1 cut(s) 577
PstNI CAGNNNCTG 1 cut(s) 747
PsyI GACNNNGTC 2 cut(s) 212, 899
PteI GCGCGC 1 cut(s) 244
PvuII CAGCTG 2 cut(s) 360, 490
RruI TCGCGA 1 cut(s) 47
RsaI GTAC 2 cut(s) 878, 954
RsaNI GTAC 2 cut(s) 877, 953
SacI GAGCTC 1 cut(s) 584
SacII CCGCGG 2 cut(s) 603, 839
SapI GCTCTTC 1 cut(s) 1040
SaqAI TTAA 4 cut(s) 507, 680, 945, 1352
SatI GCNGC 9 cut(s) 63, 158, 305, 540, 603, 637, 748, 906, 1308
Sau3AI GATC 8 cut(s) 72, 382, 420, 495, 732, 792, 948, 978
Sau96I GGNCC 9 cut(s) 13, 208, 345, 772, 819, 820, 1116, 1188, 1305
SchI GAGTC 8 cut(s) 100, 290, 547, 890, 972, 1126, 1136, 1364
ScrFI CCNGG 9 cut(s) 97, 212, 293, 445, 776, 817, 818, 874, 1629
SduI GDGCHC 4 cut(s) 584, 823, 990, 1593
SfaNI GCATC 2 cut(s) 840, 1092
SfcI CTRYAG 1 cut(s) 714
SfoI GGCGCC 1 cut(s) 560
Sfr274I CTCGAG 1 cut(s) 577
Sfr303I CCGCGG 2 cut(s) 603, 839
SfuI TTCGAA 1 cut(s) 759
SgrBI CCGCGG 2 cut(s) 603, 839
SinI GGWCC 5 cut(s) 13, 208, 345, 1116, 1188
SlaI CTCGAG 1 cut(s) 577
SmaI CCCGGG 1 cut(s) 818
SmlI CTYRAG 2 cut(s) 577, 989
SmoI CTYRAG 2 cut(s) 577, 989
SrfI GCCCGGGC 1 cut(s) 818
Sse9I AATT 5 cut(s) 135, 526, 669, 688, 1279
SspDI GGCGCC 1 cut(s) 558
SspMI CTAG 1 cut(s) 519
SstI GAGCTC 1 cut(s) 584
StyD4I CCNGG 9 cut(s) 95, 210, 291, 443, 774, 815, 816, 872, 1627
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 2 cut(s) 503, 957
TaiI ACGT 3 cut(s) 471, 756, 987
TaqII GACCGA 1 cut(s) 583
TasI AATT 5 cut(s) 135, 526, 669, 688, 1279
TauI GCSGC 5 cut(s) 160, 307, 542, 605, 1310
TfiI GAWTC 2 cut(s) 590, 1319
Tru1I TTAA 4 cut(s) 507, 680, 945, 1352
Tru9I TTAA 4 cut(s) 507, 680, 945, 1352
TscAI CASTG 2 cut(s) 85, 1033
TseFI GTSAC 2 cut(s) 595, 1244
TseI GCWGC 4 cut(s) 62, 636, 747, 905
Tsp45I GTSAC 2 cut(s) 595, 1244
TspDTI ATGAA 4 cut(s) 17, 648, 1426, 1584
TspGWI ACGGA 3 cut(s) 129, 310, 889
TspMI CCCGGG 1 cut(s) 816
TspRI CASTG 2 cut(s) 85, 1033
Tth111I GACNNNGTC 2 cut(s) 212, 899
VpaK11BI GGWCC 5 cut(s) 13, 208, 345, 1116, 1188
XagI CCTNNNNNAGG 1 cut(s) 784
XapI RAATTY 1 cut(s) 526
XcmI CCANNNNNNNNNTGG 1 cut(s) 1078
XhoI CTCGAG 1 cut(s) 577
XmaI CCCGGG 1 cut(s) 816
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.