RchiOBHm_Chr2g0170361

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0012888
Physical Location & Seq
Forward (+)
84286876 .. 84289001
2126 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53790

Sequence Viewer

Length: 1671 bp
ATGGAGTTTAAAGACGGTCTGATGAGATTAGTAGTCCACAAATCCAAAGAAACCAATTGCGTTTACGACACCGGAGATAGATTAACAGAGATAATAAGCTCTGCTCGGAGCCCAAGCCGGAGATGGAAGCTGTTTCACAGGTCCTCATCTGCAATTCCATCAAAGCCTTCAAAGCCTCAAATCCCAAGAGAGTTCATTTGCCCCATTTCCGGGTCATTAATGGGCGACCCGGTTATAGTTTCCTCGGGTCACACCTTCGAACGCGTCTGCGTCCAAGTCTGCAAAGCCCTCAACTTCACCCCTAATCTCGTGGACTCGCCCCCACCTGATCTCACCTCCGTCATCCCCAACCTCGCCCTCAAGTCCACCATCCTCAACTGGTGTCAAAATTACTCGATTGCCCCTCCCAAGCCACTTGACTCTATCTCCGCCGATAAGCTCGTCCGTACATCCATGGCTTCCGGCCACCTGAAAGTCTCGGTTTCCGAGAACGAGTCGCTGATCCGAGGCGTGGAGGAGACGCCTAACGTGAGCTTCAACCACGCGGCGACCGAGTTGACTCGTCGGCCGAGTCACTTCCACTCCAGCTCCGACGAGTCGGTCAGCGACGCTGCGTTGACCCCGCCGCTGCCGTTCTCGACTCAGCCGAGCTGCTACTCGTCGCGTTCGTCTTCGTGTTCGGAAATCGAAGCCCTGACGAATTCGAACGAGGAAGACGAAGTCTTGGCCCGGCTCAGAAGCGTCCACGTGTCCGAAATCGAGGAGGCTCTGACGTGGCTGAGGAAGATCACACGGACCAGAGAGGACGCCCGGGCCCACCTCTGCAGTCCTCGGCTCCTCTCCGCCTTGAGACCACTCGTCGTTTCCAGGCACGCCAATATTCAGGTGAACTCAGTCGCAGTTTTGGTTAACCTGTCGCTGGAAAAGTCAAACAAGATTAAGATCGTACGGTCAGGAATCCTCCCGCCTTTGATTGATGTGCTTAAGGCGGGAATGCCTGAGGCGCAGGAGCACGCCTCCGGTGTGGTCTTTAGCTTGGCGCTGGATGACGATTGCAAGACGGCTATCGGAGTATTGGGGGCTCTGCCGCCTTTGCTTCACTTGCTGAACTCGGAGGGCGAGCGGACTCGGCACGACTCGGCACTTGCTTTGTACCATTTGACATTGGTTCAGAGTAACCGGTCCAAGTTGGTTAAGATCGGGTCGGTTCCGGTTCTTTTGAGGATGGTGAAGTCGGGTCACATGACGGGTCGGGTGTTGCTCACATTGTGCAACTTGAGTTCGTGTGCGGACGGGCGGGCTGCGATGTTGGATTCAGGTGGGGTGGAGTGTTTAGTGGGGGTGTTGAGGGGGAATGAGTTTGATTCCCGGTCGACTCAGGAGAGTTGTGTGGCTACATTGTATGGGTTGAGTCGTGGGGGTTTGAGGTTTAAGGGGTTGGCGAAGGCGGCCGGGGCGGTGGAGGTGTTGAGGGAGGTGGAGAAGAGTGGGAGTGAAAGAGGGAGGGAGAAGGCGAGGAGAATGTTGGAGATGATGAGAGGGAGGGAGAAGGAGGAAGAGGAGGAAGTGGATTGGGAGGAGTTGCTTGACTCGGGGTTGGGAAGTCGAACTAGGCTTCAGTCCGGTTGTGGTGCATTGGGTGGGTCTTGTGTTAATTCGTCTGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

556

Amino Acids

60.53

Weight (kDa)

7.89

Isoelectric Point (pI)

54.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 61 - 130 3e-10 U-box domain
ARM_PUB PF25598 243 - 448 3.8e-10 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 256 - 414 1.9e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40140
fragaria_vesca FvH4_6g50090
malus_domestica MD09G1039200.v1.1 MD17G1040200.v1.1
prunus_persica Prupe.3G278800_v2.0.a1
pyrus_communis pycom111g03090 pycom17g03600
rosa_chinensis RchiOBHm_Chr2g0170361
rosa_laevigata RLG00000021949
rosa_multiflora Rmu_co8477427.1_g000001 Rmu_sc0011601.1_g000012
rosa_roxburghii Rroxscaffold_2G00081400
rosa_rugosa Rorug02G0548500
rosa_samantha Rh2AG619900 Rh2BG630900 Rh2CG600900 Rh2DG643800
rosa_wichuraiana Rw2G051360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 599
AccBSI CCGCTC 1 cut(s) 1123
AccI GTMKAC 1 cut(s) 1373
AccII CGCG 3 cut(s) 264, 545, 664
AclWI GGATC 1 cut(s) 496
AcoI YGGCCR 3 cut(s) 463, 566, 1449
AcsI RAATTY 1 cut(s) 700
AcuI CTGAAG 1 cut(s) 1601
AcvI CACGTG 1 cut(s) 748
AcyI GRCGYC 2 cut(s) 521, 807
AdeI CACNNNGTG 1 cut(s) 1269
AfaI GTAC 3 cut(s) 448, 948, 1154
AfiI CCNNNNNNNGG 4 cut(s) 209, 210, 511, 919
AflII CTTAAG 1 cut(s) 983
AflIII ACRYGT 2 cut(s) 262, 747
AgeI ACCGGT 1 cut(s) 1179
AgsI TTSAA 2 cut(s) 171, 538
AhdI GACNNNNNGTC 1 cut(s) 857
AjiI CACGTC 1 cut(s) 774
AjnI CCWGG 1 cut(s) 866
AluBI AGCT 7 cut(s) 99, 130, 439, 534, 588, 651, 1035
AluI AGCT 7 cut(s) 99, 130, 439, 534, 588, 651, 1035
Alw21I GWGCWC 1 cut(s) 1014
Alw26I GTCTC 3 cut(s) 481, 512, 844
AlwI GGATC 1 cut(s) 496
Ama87I CYCGRG 3 cut(s) 244, 810, 1591
AoxI GGCC 5 cut(s) 463, 566, 726, 813, 1449
ApaI GGGCCC 1 cut(s) 817
ApeKI GCWGC 4 cut(s) 611, 628, 651, 1301
ApoI RAATTY 1 cut(s) 700
AseI ATTAAT 1 cut(s) 218
AsiGI ACCGGT 1 cut(s) 1179
AspLEI GCGC 2 cut(s) 1006, 1042
AspS9I GGNCC 6 cut(s) 141, 727, 795, 813, 814, 1182
AsuC2I CCSGG 7 cut(s) 211, 230, 730, 811, 812, 1369, 1453
AsuHPI GGTGA 4 cut(s) 289, 325, 898, 1240
AsuII TTCGAA 2 cut(s) 258, 704
AvaI CYCGRG 3 cut(s) 244, 810, 1591
AvaII GGWCC 3 cut(s) 141, 795, 1182
AxyI CCTNAGG 1 cut(s) 999
BaeGI GKGCMC 1 cut(s) 817
BanII GRGCYC 3 cut(s) 113, 817, 1084
BauI CACGAG 1 cut(s) 308
BbrPI CACGTG 1 cut(s) 748
BbsI GAAGAC 2 cut(s) 663, 720
Bbv12I GWGCWC 1 cut(s) 1014
BbvCI CCTCAGC 1 cut(s) 779
BbvI GCAGC 4 cut(s) 598, 615, 638, 1288
BccI CCATC 4 cut(s) 117, 166, 377, 1219
BceAI ACGGC 2 cut(s) 616, 1077
BciT130I CCWGG 1 cut(s) 868
BcnI CCSGG 7 cut(s) 211, 230, 730, 811, 812, 1369, 1453
BcoDI GTCTC 3 cut(s) 481, 512, 844
BfaI CTAG 1 cut(s) 1611
BfmI CTRYAG 1 cut(s) 823
BfoI RGCGCY 1 cut(s) 1043
BfrI CTTAAG 1 cut(s) 983
BisI GCNGC 8 cut(s) 546, 612, 626, 629, 652, 1088, 1302, 1449
BlsI GCNGC 8 cut(s) 547, 613, 627, 630, 653, 1089, 1303, 1450
Bme1390I CCNGG 8 cut(s) 211, 230, 730, 811, 812, 868, 1369, 1453
Bme18I GGWCC 3 cut(s) 141, 795, 1182
BmeRI GACNNNNNGTC 1 cut(s) 857
BmeT110I CYCGRG 3 cut(s) 244, 810, 1591
BmgBI CACGTC 1 cut(s) 774
BmgT120I GGNCC 6 cut(s) 141, 727, 795, 813, 814, 1182
BmiI GGNNCC 4 cut(s) 110, 815, 836, 1209
BmrFI CCNGG 8 cut(s) 211, 230, 730, 811, 812, 868, 1369, 1453
BpiI GAAGAC 2 cut(s) 663, 720
BplI GAGNNNNNCTC 2 cut(s) 1001, 1033
BpmI CTGGAG 1 cut(s) 568
Bpu10I CCTNAGC 1 cut(s) 779
Bpu14I TTCGAA 2 cut(s) 258, 704
BpuEI CTTGAG 3 cut(s) 344, 868, 1297
BpuMI CCSGG 7 cut(s) 211, 230, 730, 811, 812, 1369, 1453
BsaAI YACGTR 1 cut(s) 748
BsaBI GATNNNNATC 1 cut(s) 941
BsaHI GRCGYC 2 cut(s) 521, 807
BsaI GGTCTC 1 cut(s) 844
BsaJI CCNNGG 6 cut(s) 243, 453, 505, 810, 830, 1452
BsaWI WCCGGW 5 cut(s) 71, 1019, 1179, 1210, 1622
BsaXI ACNNNNNCTCC 6 cut(s) 410, 440, 566, 572, 596, 602
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 210, 511, 919
Bse118I RCCGGY 1 cut(s) 1179
Bse1I ACTGG 1 cut(s) 383
Bse21I CCTNAGG 1 cut(s) 999
Bse8I GATNNNNATC 1 cut(s) 941
BseBI CCWGG 1 cut(s) 868
BseDI CCNNGG 6 cut(s) 243, 453, 505, 810, 830, 1452
BseGI GGATG 5 cut(s) 342, 369, 449, 1051, 1230
BseJI GATNNNNATC 1 cut(s) 941
BseLI CCNNNNNNNGG 4 cut(s) 209, 210, 511, 919
BseMII CTCAG 7 cut(s) 656, 748, 770, 906, 990, 1391, 1653
BseNI ACTGG 1 cut(s) 383
BseRI GAGGAG 6 cut(s) 530, 776, 827, 1531, 1574, 1592
BseSI GKGCMC 1 cut(s) 817
BseX3I CGGCCG 2 cut(s) 566, 1449
BseXI GCAGC 4 cut(s) 598, 615, 638, 1288
Bsh1236I CGCG 3 cut(s) 264, 545, 664
Bsh1285I CGRYCG 4 cut(s) 552, 569, 1373, 1452
BshFI GGCC 5 cut(s) 465, 568, 728, 815, 1451
BshTI ACCGGT 1 cut(s) 1179
BsiEI CGRYCG 4 cut(s) 552, 569, 1373, 1452
BsiHKAI GWGCWC 1 cut(s) 1014
BsiHKCI CYCGRG 3 cut(s) 244, 810, 1591
BsiWI CGTACG 1 cut(s) 946
BslI CCNNNNNNNGG 4 cut(s) 209, 210, 511, 919
BsmAI GTCTC 3 cut(s) 481, 512, 844
BsmBI CGTCTC 1 cut(s) 512
BsmI GAATGC 1 cut(s) 999
BsnI GGCC 5 cut(s) 465, 568, 728, 815, 1451
Bso31I GGTCTC 1 cut(s) 844
BsoBI CYCGRG 3 cut(s) 244, 810, 1591
Bsp119I TTCGAA 2 cut(s) 258, 704
Bsp120I GGGCCC 1 cut(s) 813
Bsp1286I GDGCHC 4 cut(s) 113, 817, 1014, 1084
Bsp143I GATC 5 cut(s) 328, 501, 786, 942, 1197
Bsp19I CCATGG 1 cut(s) 453
BspANI GGCC 5 cut(s) 465, 568, 728, 815, 1451
BspCNI CTCAG 7 cut(s) 655, 747, 771, 905, 991, 1390, 1654
BspFNI CGCG 3 cut(s) 264, 545, 664
BspLI GGNNCC 4 cut(s) 110, 815, 836, 1209
BspMAI CTGCAG 1 cut(s) 827
BspPI GGATC 1 cut(s) 496
BspT104I TTCGAA 2 cut(s) 258, 704
BspTI CTTAAG 1 cut(s) 983
BspTNI GGTCTC 1 cut(s) 844
BsrBI CCGCTC 1 cut(s) 1123
BsrFI RCCGGY 1 cut(s) 1179
BsrI ACTGG 1 cut(s) 383
BssAI RCCGGY 1 cut(s) 1179
BssECI CCNNGG 6 cut(s) 243, 453, 505, 810, 830, 1452
BssMI GATC 5 cut(s) 328, 501, 786, 942, 1197
BssNI GRCGYC 2 cut(s) 521, 807
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 868
Bst4CI ACNGT 2 cut(s) 17, 951
Bst6I CTCTTC 2 cut(s) 1478, 1551
BstACI GRCGYC 2 cut(s) 521, 807
BstAFI CTTAAG 1 cut(s) 983
BstBAI YACGTR 1 cut(s) 748
BstBI TTCGAA 2 cut(s) 258, 704
BstC8I GCNNGC 4 cut(s) 873, 1014, 1121, 1299
BstDEI CTNAG 7 cut(s) 642, 734, 779, 892, 999, 1377, 1662
BstDSI CCRYGG 1 cut(s) 453
BstF5I GGATG 5 cut(s) 342, 369, 449, 1051, 1230
BstFNI CGCG 3 cut(s) 264, 545, 664
BstH2I RGCGCY 1 cut(s) 1043
BstHHI GCGC 2 cut(s) 1006, 1042
BstKTI GATC 5 cut(s) 331, 504, 789, 945, 1200
BstMAI GTCTC 3 cut(s) 481, 512, 844
BstMBI GATC 5 cut(s) 328, 501, 786, 942, 1197
BstMCI CGRYCG 4 cut(s) 552, 569, 1373, 1452
BstMWI GCNNNNNNNGC 7 cut(s) 172, 1003, 1093, 1102, 1129, 1448, 1454
BstNI CCWGG 1 cut(s) 868
BstSCI CCNGG 8 cut(s) 209, 228, 728, 809, 810, 866, 1367, 1451
BstSFI CTRYAG 1 cut(s) 823
BstSLI GKGCMC 1 cut(s) 817
BstUI CGCG 3 cut(s) 264, 545, 664
BstV1I GCAGC 4 cut(s) 598, 615, 638, 1288
BstV2I GAAGAC 2 cut(s) 663, 720
BstZI CGGCCG 2 cut(s) 566, 1449
Bsu36I CCTNAGG 1 cut(s) 999
BsuRI GGCC 5 cut(s) 465, 568, 728, 815, 1451
BtgI CCRYGG 1 cut(s) 453
BtgZI GCGATG 1 cut(s) 1319
BtrI CACGTC 1 cut(s) 774
BtsCI GGATG 5 cut(s) 342, 369, 449, 1051, 1230
Cac8I GCNNGC 4 cut(s) 873, 1014, 1121, 1299
CfoI GCGC 2 cut(s) 1006, 1042
Cfr10I RCCGGY 1 cut(s) 1179
Cfr13I GGNCC 6 cut(s) 141, 727, 795, 813, 814, 1182
Cfr9I CCCGGG 1 cut(s) 810
CseI GACGC 6 cut(s) 253, 259, 529, 617, 730, 815
Csp6I GTAC 3 cut(s) 447, 947, 1153
CspAI ACCGGT 1 cut(s) 1179
CviAII CATG 2 cut(s) 454, 1243
CviQI GTAC 3 cut(s) 447, 947, 1153
DdeI CTNAG 7 cut(s) 642, 734, 779, 892, 999, 1377, 1662
DpnI GATC 5 cut(s) 330, 503, 788, 944, 1199
DpnII GATC 5 cut(s) 328, 501, 786, 942, 1197
DraI TTTAAA 1 cut(s) 10
DraIII CACNNNGTG 1 cut(s) 1269
DrdI GACNNNNNNGTC 1 cut(s) 599
DriI GACNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 599
EaeI YGGCCR 3 cut(s) 463, 566, 1449
EagI CGGCCG 2 cut(s) 566, 1449
Eam1104I CTCTTC 2 cut(s) 1478, 1551
Eam1105I GACNNNNNGTC 1 cut(s) 857
EarI CTCTTC 2 cut(s) 1478, 1551
EciI GGCGGA 2 cut(s) 418, 832
EclXI CGGCCG 2 cut(s) 566, 1449
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 3 cut(s) 113, 817, 1084
Eco31I GGTCTC 1 cut(s) 844
Eco47I GGWCC 3 cut(s) 141, 795, 1182
Eco52I CGGCCG 2 cut(s) 566, 1449
Eco57I CTGAAG 1 cut(s) 1601
Eco72I CACGTG 1 cut(s) 748
Eco81I CCTNAGG 1 cut(s) 999
Eco88I CYCGRG 3 cut(s) 244, 810, 1591
EcoO109I RGGNCCY 1 cut(s) 141
EcoRI GAATTC 1 cut(s) 700
EcoRII CCWGG 1 cut(s) 866
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 3 cut(s) 113, 817, 1084
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 512
FaeI CATG 2 cut(s) 457, 1246
FaiI YATR 4 cut(s) 236, 455, 1244, 1404
FatI CATG 2 cut(s) 453, 1242
FauI CCCGC 4 cut(s) 630, 972, 982, 1290
FblI GTMKAC 1 cut(s) 1373
Fnu4HI GCNGC 8 cut(s) 546, 612, 626, 629, 652, 1088, 1302, 1449
FokI GGATG 5 cut(s) 329, 356, 436, 1058, 1237
FriOI GRGCYC 3 cut(s) 113, 817, 1084
Fsp4HI GCNGC 8 cut(s) 546, 612, 626, 629, 652, 1088, 1302, 1449
FspBI CTAG 1 cut(s) 1611
GlaI GCGC 2 cut(s) 1005, 1041
GluI GCNGC 8 cut(s) 546, 612, 626, 629, 652, 1088, 1302, 1449
GsuI CTGGAG 1 cut(s) 568
HaeII RGCGCY 1 cut(s) 1043
HaeIII GGCC 5 cut(s) 465, 568, 728, 815, 1451
HgaI GACGC 6 cut(s) 253, 259, 529, 617, 730, 815
HhaI GCGC 2 cut(s) 1006, 1042
Hin1I GRCGYC 2 cut(s) 521, 807
Hin1II CATG 2 cut(s) 457, 1246
Hin6I GCGC 2 cut(s) 1004, 1040
HinP1I GCGC 2 cut(s) 1004, 1040
HincII GTYRAC 4 cut(s) 558, 618, 910, 1374
HindII GTYRAC 4 cut(s) 558, 618, 910, 1374
HpaI GTTAAC 1 cut(s) 910
HphI GGTGA 4 cut(s) 289, 325, 898, 1240
Hpy188III TCNNGA 3 cut(s) 637, 954, 1379
Hpy99I CGWCG 5 cut(s) 567, 596, 611, 664, 863
HpyAV CCTTC 5 cut(s) 177, 265, 1438, 1504, 1543
HpyCH4III ACNGT 2 cut(s) 17, 951
HpyCH4IV ACGT 3 cut(s) 528, 747, 773
HpyCH4V TGCA 6 cut(s) 152, 282, 825, 1056, 1272, 1634
HpyF10VI GCNNNNNNNGC 7 cut(s) 172, 1003, 1093, 1102, 1129, 1448, 1454
HpyF3I CTNAG 7 cut(s) 642, 734, 779, 892, 999, 1377, 1662
HpySE526I ACGT 3 cut(s) 528, 747, 773
Hsp92I GRCGYC 2 cut(s) 521, 807
Hsp92II CATG 2 cut(s) 457, 1246
HspAI GCGC 2 cut(s) 1004, 1040
KspAI GTTAAC 1 cut(s) 910
Kzo9I GATC 5 cut(s) 328, 501, 786, 942, 1197
LmnI GCTCC 4 cut(s) 108, 593, 840, 1009
Lsp1109I GCAGC 4 cut(s) 598, 615, 638, 1288
MaeI CTAG 1 cut(s) 1611
MaeII ACGT 3 cut(s) 528, 747, 773
MaeIII GTNAC 4 cut(s) 248, 572, 1175, 1238
MalI GATC 5 cut(s) 330, 503, 788, 944, 1199
MbiI CCGCTC 1 cut(s) 1123
MboI GATC 5 cut(s) 328, 501, 786, 942, 1197
MboII GAAGA 5 cut(s) 663, 725, 796, 1495, 1568
MfeI CAATTG 1 cut(s) 55
MhlI GDGCHC 4 cut(s) 113, 817, 1014, 1084
MluCI AATT 5 cut(s) 55, 153, 388, 700, 1654
MluI ACGCGT 1 cut(s) 262
MmeI TCCRAC 3 cut(s) 615, 1290, 1506
MseI TTAA 9 cut(s) 9, 83, 218, 909, 939, 984, 1194, 1431, 1653
MspA1I CMGCKG 1 cut(s) 628
MspCI CTTAAG 1 cut(s) 983
MspR9I CCNGG 8 cut(s) 211, 230, 730, 811, 812, 868, 1369, 1453
MunI CAATTG 1 cut(s) 55
Mva1269I GAATGC 1 cut(s) 999
MvaI CCWGG 1 cut(s) 868
MvnI CGCG 3 cut(s) 264, 545, 664
MwoI GCNNNNNNNGC 7 cut(s) 172, 1003, 1093, 1102, 1129, 1448, 1454
NciI CCSGG 7 cut(s) 211, 230, 730, 811, 812, 1369, 1453
NcoI CCATGG 1 cut(s) 453
NdeII GATC 5 cut(s) 328, 501, 786, 942, 1197
NlaIII CATG 2 cut(s) 457, 1246
NlaIV GGNNCC 4 cut(s) 110, 815, 836, 1209
NmeAIII GCCGAG 5 cut(s) 594, 672, 811, 1108, 1118
NmuCI GTSAC 3 cut(s) 248, 572, 1238
NspV TTCGAA 2 cut(s) 258, 704
PcsI WCGNNNNNNNCGW 4 cut(s) 549, 644, 665, 714
PctI GAATGC 1 cut(s) 999
PfeI GAWTC 3 cut(s) 957, 1313, 1364
Pfl23II CGTACG 1 cut(s) 946
PflFI GACNNNGTC 1 cut(s) 719
PinAI ACCGGT 1 cut(s) 1179
PkrI GCNGC 8 cut(s) 547, 613, 627, 630, 653, 1089, 1303, 1450
PmaCI CACGTG 1 cut(s) 748
PmlI CACGTG 1 cut(s) 748
Ppu21I YACGTR 1 cut(s) 748
PpuMI RGGWCCY 1 cut(s) 141
PshBI ATTAAT 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 866
PspCI CACGTG 1 cut(s) 748
PspGI CCWGG 1 cut(s) 866
PspLI CGTACG 1 cut(s) 946
PspN4I GGNNCC 4 cut(s) 110, 815, 836, 1209
PspOMI GGGCCC 1 cut(s) 813
PspPI GGNCC 6 cut(s) 141, 727, 795, 813, 814, 1182
PspPPI RGGWCCY 1 cut(s) 141
PstI CTGCAG 1 cut(s) 827
PsyI GACNNNGTC 1 cut(s) 719
RsaI GTAC 3 cut(s) 448, 948, 1154
RsaNI GTAC 3 cut(s) 447, 947, 1153
SalI GTCGAC 1 cut(s) 1372
SaqAI TTAA 9 cut(s) 9, 83, 218, 909, 939, 984, 1194, 1431, 1653
SatI GCNGC 8 cut(s) 546, 612, 626, 629, 652, 1088, 1302, 1449
Sau3AI GATC 5 cut(s) 328, 501, 786, 942, 1197
Sau96I GGNCC 6 cut(s) 141, 727, 795, 813, 814, 1182
ScrFI CCNGG 8 cut(s) 211, 230, 730, 811, 812, 868, 1369, 1453
SduI GDGCHC 4 cut(s) 113, 817, 1014, 1084
SfcI CTRYAG 1 cut(s) 823
SfuI TTCGAA 2 cut(s) 258, 704
SinI GGWCC 3 cut(s) 141, 795, 1182
SmaI CCCGGG 1 cut(s) 812
SmlI CTYRAG 4 cut(s) 359, 847, 983, 1276
SmoI CTYRAG 4 cut(s) 359, 847, 983, 1276
SrfI GCCCGGGC 1 cut(s) 812
Sse9I AATT 5 cut(s) 55, 153, 388, 700, 1654
SspI AATATT 1 cut(s) 880
SspMI CTAG 1 cut(s) 1611
StyD4I CCNGG 8 cut(s) 209, 228, 728, 809, 810, 866, 1367, 1451
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 2 cut(s) 17, 951
TaiI ACGT 3 cut(s) 531, 750, 776
TaqI TCGA 8 cut(s) 258, 395, 638, 687, 704, 759, 1373, 1606
TaqII GACCGA 2 cut(s) 566, 589
TasI AATT 5 cut(s) 55, 153, 388, 700, 1654
TauI GCSGC 4 cut(s) 548, 628, 1090, 1451
TfiI GAWTC 3 cut(s) 957, 1313, 1364
Tru1I TTAA 9 cut(s) 9, 83, 218, 909, 939, 984, 1194, 1431, 1653
Tru9I TTAA 9 cut(s) 9, 83, 218, 909, 939, 984, 1194, 1431, 1653
TseFI GTSAC 3 cut(s) 248, 572, 1238
TseI GCWGC 4 cut(s) 611, 628, 651, 1301
Tsp45I GTSAC 3 cut(s) 248, 572, 1238
TspDTI ATGAA 1 cut(s) 184
TspGWI ACGGA 3 cut(s) 328, 434, 808
TspMI CCCGGG 1 cut(s) 810
Tth111I GACNNNGTC 1 cut(s) 719
Vha464I CTTAAG 1 cut(s) 983
VpaK11BI GGWCC 3 cut(s) 141, 795, 1182
VspI ATTAAT 1 cut(s) 218
XapI RAATTY 1 cut(s) 700
XcmI CCANNNNNNNNNTGG 1 cut(s) 120
XmaI CCCGGG 1 cut(s) 810
XmiI GTMKAC 1 cut(s) 1373
XspI CTAG 1 cut(s) 1611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.