MD17G1040200.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012888
Physical Location & Seq
Reverse (-)
2933388 .. 2935043
1656 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1040200.v1.1.491

Sequence Viewer

Length: 1656 bp
ATGAAGCTGATGGTCCACAAATCAAAAGAGAACGAATGGGCTTTCGCGACGCCGGAAAGAGCTGCGGAGATGAGCAGTGCAAGCATGAGTCCCCGGCGGAGATGGAAGCTTTTTCAGAGGTCGTCGTCGGCAATTCCGTCGAAAACTTCAAAGCCGCAAGCTCCCAAAGAGTTCGTCTGTCCAATCTCCGGTTCTCTAATGGCGGACCCGGTCATCGTTTCGTCCGGCCACACCTTCGAGCGCGCCTGCGTCCAAGCCTGCAAGTCCCTCAGCTTCACGCCTGTTCTTCCGGACTCTCCGCCGCCAGATTTCTCCTCCGTAATCTCCAACCTCGCCCTCAGGTCCGCCATTCTCAGCTGGTGCCACAAGTCCTCCGTCGATCCTCCCAAGCCCCTCGATTTCGCCTCCGCCGAGAAGATCGTCCGCGCCTCCATTGGTTCCCGGAACGAAACTAAGCCCCAGAACGTCGTAGTTTCGGATAATCAGCTGTTGATCAACCCTACAGTGAATTTCACTCAGGCGGCCACCGAGCAGACTCGACTCCCAACTCACTTCCCCTCGAGCTCCGACGAATCGGTCTCCGCGGCTCCGCCTCTCCCGTTCTCAACTCCACCGAGATGCTACTCCTCCCCGTCGTCTTCATCCTCGGAACTTGAAACTTTAAATCCCAACTGCAACAGCAATCCCTCCCTCTCCGTAGAAGACGAAATCCCGATCAAGCTCAGAAGCTGCCACGTGTTCGAAATCGAGGAGGCCCTGGCCTCGCTCAGGAATATCACGCGGACGAGAGAGGACGCCCGGGCCCGTCTCTGCACCCCGCGGTTGCTCTCCGCCCTCCGCCCTCTGATTACATCCAGGTACACCGCCGTTCAGGTGGACTCCGTCGCAGCGTTGGTCAATCTGTCGCTGGAAAAGTCAAACAAGATTAAGATCGTACGGTCAGGAGTCCTCCCTCCTCTGATCGACGTGCTCAAGGCCGGAACCCCTGAGGCGCAGGAACACGCTTCCAGTGCGCTCTTCAGCTTGGCACTCGACGACGACTGCAAGACCGCCATTGGTGTTTTGGGCGCGTTGCCGCCATTGCTGCACTTACTCCGGTCCGAGAGCGAGCGGACTCGGCATGACTCGGCACTCGCTTTGTACCACCTCTCACTCGTCCAGAGCAACCGGTCCAAGTTGGTTAAGCTCGGCTCGGTTCCGGTTCTTCTGAGAATGTTGAAGTCGGGTCACATGACCGGTCGGGTATTGCTCACATTGTGTAATTTGAGTTTGTGTGCCGACGGGCGGGCCGCATTGTTGGATTCTGGTGGGGTGGAGTGTTTGGTGGGCGTGTTAAGGGGAAACGAGTTTGACTCGAAGGCGACACAAGAGAGTTGTGTGGCCACAATGTATGGGTTGAGCTACGGCGGTTTGAGGTTCAAGGGGCTGGCGAAGAAGGCGGGCGTGGTGGAGGTGTTGAGAGAGGTGGAGAAAAAGGGAAGCGAACGGGCGAGGGAGAAAGCGAGGCGGATGTTGGAGATGATGAGAGGGAAAGAGAAGGAAGAAGAAGATGAAGTGGATTGGGCGGAATTGCTCAACTCGGGGTTTGGGAGCCGAACTCGGTGTCAACCCGGTGGTGGACTGGACGAGTCGAGTGTCAACTCATTGGACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

552

Amino Acids

59.88

Weight (kDa)

8.67

Isoelectric Point (pI)

54.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 54 - 123 1.4e-10 U-box domain
ARM_PUB PF25598 250 - 513 9.8e-10 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 254 - 410 2.7e-09 ARMC5
Arm PF00514 345 - 384 9.6e-07 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 358 - 486 1.4e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40140
fragaria_vesca FvH4_6g50090
malus_domestica MD09G1039200.v1.1 MD17G1040200.v1.1
prunus_persica Prupe.3G278800_v2.0.a1
pyrus_communis pycom111g03090 pycom17g03600
rosa_chinensis RchiOBHm_Chr2g0170361
rosa_laevigata RLG00000021949
rosa_multiflora Rmu_co8477427.1_g000001 Rmu_sc0011601.1_g000012
rosa_roxburghii Rroxscaffold_2G00081400
rosa_rugosa Rorug02G0548500
rosa_samantha Rh2AG619900 Rh2BG630900 Rh2CG600900 Rh2DG643800
rosa_wichuraiana Rw2G051360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 575
AccB1I GGYRCC 1 cut(s) 360
AccBSI CCGCTC 1 cut(s) 1111
AccII CGCG 7 cut(s) 47, 243, 426, 584, 781, 820, 1070
AccIII TCCGGA 1 cut(s) 289
AclWI GGATC 1 cut(s) 374
AcoI YGGCCR 3 cut(s) 226, 522, 1380
AcsI RAATTY 1 cut(s) 508
AcuI CTGAAG 1 cut(s) 1003
AcvI CACGTG 1 cut(s) 736
AcyI GRCGYC 2 cut(s) 50, 795
AdeI CACNNNGTG 1 cut(s) 1257
AfaI GTAC 3 cut(s) 860, 936, 1142
AfiI CCNNNNNNNGG 4 cut(s) 188, 768, 1284, 1616
AflIII ACRYGT 1 cut(s) 735
AgeI ACCGGT 2 cut(s) 1167, 1235
AgsI TTSAA 4 cut(s) 150, 656, 1219, 1420
AjiI CACGTC 1 cut(s) 967
AjnI CCWGG 2 cut(s) 756, 854
AjuI GAANNNNNNNTTGG 2 cut(s) 175, 207
Alw21I GWGCWC 2 cut(s) 566, 972
Alw26I GTCTC 2 cut(s) 583, 812
AlwI GGATC 1 cut(s) 374
AlwNI CAGNNNCTG 1 cut(s) 729
Ama87I CYCGRG 3 cut(s) 559, 798, 1579
Aor13HI TCCGGA 1 cut(s) 289
AoxI GGCC 8 cut(s) 226, 522, 753, 759, 801, 975, 1287, 1380
ApaI GGGCCC 1 cut(s) 805
ApeKI GCWGC 4 cut(s) 62, 729, 887, 1084
ApoI RAATTY 1 cut(s) 508
ArsI GACNNNNNNTTYG 4 cut(s) 159, 191, 1588, 1620
AsiGI ACCGGT 2 cut(s) 1167, 1235
AspLEI GCGC 6 cut(s) 243, 245, 428, 994, 1015, 1070
AspS9I GGNCC 9 cut(s) 13, 205, 342, 754, 801, 802, 1098, 1170, 1287
AsuC2I CCSGG 6 cut(s) 94, 209, 442, 799, 800, 1611
AsuII TTCGAA 1 cut(s) 741
AvaI CYCGRG 3 cut(s) 559, 798, 1579
AvaII GGWCC 5 cut(s) 13, 205, 342, 1098, 1170
AxyI CCTNAGG 2 cut(s) 338, 987
BaeGI GKGCMC 1 cut(s) 805
BalI TGGCCA 1 cut(s) 1382
BanI GGYRCC 1 cut(s) 360
BanII GRGCYC 2 cut(s) 566, 805
BbrPI CACGTG 1 cut(s) 736
BbsI GAAGAC 2 cut(s) 630, 708
Bbv12I GWGCWC 2 cut(s) 566, 972
BbvCI CCTCAGC 1 cut(s) 269
BbvI GCAGC 4 cut(s) 49, 716, 899, 1071
BccI CCATC 2 cut(s) 4, 96
BceAI ACGGC 2 cut(s) 851, 1420
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BciT130I CCWGG 2 cut(s) 758, 856
BclI TGATCA 1 cut(s) 492
BcnI CCSGG 6 cut(s) 94, 209, 442, 799, 800, 1611
BcoDI GTCTC 2 cut(s) 583, 812
BfmI CTRYAG 1 cut(s) 501
Bme1390I CCNGG 8 cut(s) 94, 209, 442, 758, 799, 800, 856, 1611
Bme18I GGWCC 5 cut(s) 13, 205, 342, 1098, 1170
BmeT110I CYCGRG 3 cut(s) 559, 798, 1579
BmgBI CACGTC 1 cut(s) 967
BmgT120I GGNCC 9 cut(s) 13, 205, 342, 754, 801, 802, 1098, 1170, 1287
BmiI GGNNCC 8 cut(s) 207, 362, 439, 588, 803, 982, 1197, 1592
BmrFI CCNGG 8 cut(s) 94, 209, 442, 758, 799, 800, 856, 1611
BmsI GCATC 1 cut(s) 608
BpiI GAAGAC 2 cut(s) 630, 708
BplI GAGNNNNNCTC 4 cut(s) 1337, 1369, 1582, 1614
Bpu10I CCTNAGC 2 cut(s) 269, 767
Bpu14I TTCGAA 1 cut(s) 741
BpuEI CTTGAG 1 cut(s) 956
BpuMI CCSGG 6 cut(s) 94, 209, 442, 799, 800, 1611
BsaAI YACGTR 1 cut(s) 736
BsaBI GATNNNNATC 1 cut(s) 929
BsaHI GRCGYC 2 cut(s) 50, 795
BsaI GGTCTC 1 cut(s) 583
BsaJI CCNNGG 6 cut(s) 92, 582, 645, 756, 798, 818
BsaWI WCCGGW 6 cut(s) 188, 289, 1095, 1167, 1198, 1235
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 4 cut(s) 188, 768, 1284, 1616
Bse118I RCCGGY 2 cut(s) 1167, 1235
Bse1I ACTGG 2 cut(s) 1008, 1626
Bse21I CCTNAGG 2 cut(s) 338, 987
Bse3DI GCAATG 1 cut(s) 1079
Bse8I GATNNNNATC 1 cut(s) 929
BseAI TCCGGA 1 cut(s) 289
BseBI CCWGG 2 cut(s) 758, 856
BseDI CCNNGG 6 cut(s) 92, 582, 645, 756, 798, 818
BseGI GGATG 3 cut(s) 641, 851, 1515
BseJI GATNNNNATC 1 cut(s) 929
BseLI CCNNNNNNNGG 4 cut(s) 188, 768, 1284, 1616
BseMI GCAATG 1 cut(s) 1079
BseMII CTCAG 8 cut(s) 283, 352, 367, 530, 736, 781, 978, 1199
BseNI ACTGG 2 cut(s) 1008, 1626
BsePI GCGCGC 1 cut(s) 241
BseRI GAGGAG 4 cut(s) 304, 616, 764, 945
BseSI GKGCMC 1 cut(s) 805
BseXI GCAGC 4 cut(s) 49, 716, 899, 1071
BsgI GTGCAG 2 cut(s) 796, 1070
Bsh1236I CGCG 7 cut(s) 47, 243, 426, 584, 781, 820, 1070
Bsh1285I CGRYCG 1 cut(s) 1240
BshFI GGCC 8 cut(s) 228, 524, 755, 761, 803, 977, 1289, 1382
BshNI GGYRCC 1 cut(s) 360
BshTI ACCGGT 2 cut(s) 1167, 1235
BsiEI CGRYCG 1 cut(s) 1240
BsiHKAI GWGCWC 2 cut(s) 566, 972
BsiHKCI CYCGRG 3 cut(s) 559, 798, 1579
BsiWI CGTACG 1 cut(s) 934
BslFI GGGAC 2 cut(s) 75, 250
BslI CCNNNNNNNGG 4 cut(s) 188, 768, 1284, 1616
BsmAI GTCTC 2 cut(s) 583, 812
BsmBI CGTCTC 1 cut(s) 812
BsmFI GGGAC 2 cut(s) 75, 250
BsnI GGCC 8 cut(s) 228, 524, 755, 761, 803, 977, 1289, 1382
Bso31I GGTCTC 1 cut(s) 583
BsoBI CYCGRG 3 cut(s) 559, 798, 1579
Bsp119I TTCGAA 1 cut(s) 741
Bsp120I GGGCCC 1 cut(s) 801
Bsp1286I GDGCHC 3 cut(s) 566, 805, 972
Bsp13I TCCGGA 1 cut(s) 289
Bsp143I GATC 6 cut(s) 379, 417, 492, 714, 930, 960
Bsp68I TCGCGA 1 cut(s) 47
BspANI GGCC 8 cut(s) 228, 524, 755, 761, 803, 977, 1289, 1382
BspCNI CTCAG 8 cut(s) 282, 351, 366, 529, 735, 780, 979, 1200
BspEI TCCGGA 1 cut(s) 289
BspFNI CGCG 7 cut(s) 47, 243, 426, 584, 781, 820, 1070
BspLI GGNNCC 8 cut(s) 207, 362, 439, 588, 803, 982, 1197, 1592
BspPI GGATC 1 cut(s) 374
BspQI GCTCTTC 1 cut(s) 1022
BspT104I TTCGAA 1 cut(s) 741
BspT107I GGYRCC 1 cut(s) 360
BspTNI GGTCTC 1 cut(s) 583
BsrBI CCGCTC 1 cut(s) 1111
BsrDI GCAATG 1 cut(s) 1079
BsrFI RCCGGY 2 cut(s) 1167, 1235
BsrI ACTGG 2 cut(s) 1008, 1626
BssAI RCCGGY 2 cut(s) 1167, 1235
BssECI CCNNGG 6 cut(s) 92, 582, 645, 756, 798, 818
BssHII GCGCGC 1 cut(s) 241
BssMI GATC 6 cut(s) 379, 417, 492, 714, 930, 960
BssNI GRCGYC 2 cut(s) 50, 795
Bst2UI CCWGG 2 cut(s) 758, 856
Bst4CI ACNGT 2 cut(s) 505, 939
Bst6I CTCTTC 1 cut(s) 1022
BstACI GRCGYC 2 cut(s) 50, 795
BstBAI YACGTR 1 cut(s) 736
BstBI TTCGAA 1 cut(s) 741
BstC8I GCNNGC 9 cut(s) 82, 159, 243, 247, 259, 1109, 1287, 1428, 1441
BstDEI CTNAG 9 cut(s) 269, 338, 353, 453, 516, 722, 767, 987, 1208
BstDSI CCRYGG 2 cut(s) 582, 818
BstENI CCTNNNNNAGG 1 cut(s) 766
BstF5I GGATG 3 cut(s) 641, 851, 1515
BstFNI CGCG 7 cut(s) 47, 243, 426, 584, 781, 820, 1070
BstHHI GCGC 6 cut(s) 243, 245, 428, 994, 1015, 1070
BstKTI GATC 6 cut(s) 382, 420, 495, 717, 933, 963
BstMAI GTCTC 2 cut(s) 583, 812
BstMBI GATC 6 cut(s) 379, 417, 492, 714, 930, 960
BstMCI CGRYCG 1 cut(s) 1240
BstMWI GCNNNNNNNGC 6 cut(s) 81, 1010, 1081, 1084, 1117, 1436
BstNI CCWGG 2 cut(s) 758, 856
BstSCI CCNGG 8 cut(s) 92, 207, 440, 756, 797, 798, 854, 1609
BstSFI CTRYAG 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 805
BstUI CGCG 7 cut(s) 47, 243, 426, 584, 781, 820, 1070
BstV1I GCAGC 4 cut(s) 49, 716, 899, 1071
BstV2I GAAGAC 2 cut(s) 630, 708
Bsu36I CCTNAGG 2 cut(s) 338, 987
BsuRI GGCC 8 cut(s) 228, 524, 755, 761, 803, 977, 1289, 1382
BtgI CCRYGG 2 cut(s) 582, 818
BtrI CACGTC 1 cut(s) 967
BtsCI GGATG 3 cut(s) 641, 851, 1515
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 3 cut(s) 82, 510, 1015
BtuMI TCGCGA 1 cut(s) 47
Cac8I GCNNGC 9 cut(s) 82, 159, 243, 247, 259, 1109, 1287, 1428, 1441
CaiI CAGNNNCTG 1 cut(s) 729
CfoI GCGC 6 cut(s) 243, 245, 428, 994, 1015, 1070
Cfr10I RCCGGY 2 cut(s) 1167, 1235
Cfr13I GGNCC 9 cut(s) 13, 205, 342, 754, 801, 802, 1098, 1170, 1287
Cfr42I CCGCGG 2 cut(s) 585, 821
Cfr9I CCCGGG 1 cut(s) 798
CpoI CGGWCCG 1 cut(s) 1098
CseI GACGC 3 cut(s) 58, 238, 803
Csp6I GTAC 3 cut(s) 859, 935, 1141
CspAI ACCGGT 2 cut(s) 1167, 1235
CspI CGGWCCG 1 cut(s) 1098
CviAII CATG 3 cut(s) 85, 1121, 1231
CviQI GTAC 3 cut(s) 859, 935, 1141
DdeI CTNAG 9 cut(s) 269, 338, 353, 453, 516, 722, 767, 987, 1208
DpnI GATC 6 cut(s) 381, 419, 494, 716, 932, 962
DpnII GATC 6 cut(s) 379, 417, 492, 714, 930, 960
DraI TTTAAA 1 cut(s) 663
DraIII CACNNNGTG 1 cut(s) 1257
DrdI GACNNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 1 cut(s) 575
EaeI YGGCCR 3 cut(s) 226, 522, 1380
Eam1104I CTCTTC 1 cut(s) 1022
EarI CTCTTC 1 cut(s) 1022
Ecl136II GAGCTC 1 cut(s) 564
Eco24I GRGCYC 2 cut(s) 566, 805
Eco31I GGTCTC 1 cut(s) 583
Eco47I GGWCC 5 cut(s) 13, 205, 342, 1098, 1170
Eco53kI GAGCTC 1 cut(s) 564
Eco57I CTGAAG 1 cut(s) 1003
Eco72I CACGTG 1 cut(s) 736
Eco81I CCTNAGG 2 cut(s) 338, 987
Eco88I CYCGRG 3 cut(s) 559, 798, 1579
EcoICRI GAGCTC 1 cut(s) 564
EcoNI CCTNNNNNAGG 1 cut(s) 766
EcoO109I RGGNCCY 1 cut(s) 754
EcoRII CCWGG 2 cut(s) 756, 854
EcoT38I GRGCYC 2 cut(s) 566, 805
Esp3I CGTCTC 1 cut(s) 812
FaeI CATG 3 cut(s) 88, 1124, 1234
FaiI YATR 4 cut(s) 86, 1122, 1232, 1392
FaqI GGGAC 2 cut(s) 75, 250
FatI CATG 3 cut(s) 84, 1120, 1230
FauI CCCGC 3 cut(s) 825, 1278, 1432
FbaI TGATCA 1 cut(s) 492
FokI GGATG 3 cut(s) 628, 838, 1522
FriOI GRGCYC 2 cut(s) 566, 805
GlaI GCGC 6 cut(s) 242, 244, 427, 993, 1014, 1069
HaeIII GGCC 8 cut(s) 228, 524, 755, 761, 803, 977, 1289, 1382
HgaI GACGC 3 cut(s) 58, 238, 803
HhaI GCGC 6 cut(s) 243, 245, 428, 994, 1015, 1070
Hin1I GRCGYC 2 cut(s) 50, 795
Hin1II CATG 3 cut(s) 88, 1124, 1234
Hin6I GCGC 6 cut(s) 241, 243, 426, 992, 1013, 1068
HinP1I GCGC 6 cut(s) 241, 243, 426, 992, 1013, 1068
HincII GTYRAC 2 cut(s) 1607, 1639
HindII GTYRAC 2 cut(s) 1607, 1639
HindIII AAGCTT 1 cut(s) 107
Hpy166II GTNNAC 6 cut(s) 16, 861, 877, 1607, 1619, 1639
Hpy188I TCNGA 9 cut(s) 117, 478, 568, 649, 725, 846, 960, 1102, 1209
Hpy188III TCNNGA 6 cut(s) 46, 290, 712, 769, 942, 1159
Hpy8I GTNNAC 6 cut(s) 16, 861, 877, 1607, 1619, 1639
HpyAV CCTTC 4 cut(s) 244, 1351, 1429, 1531
HpyCH4III ACNGT 2 cut(s) 505, 939
HpyCH4IV ACGT 3 cut(s) 465, 735, 966
HpyCH4V TGCA 6 cut(s) 80, 261, 675, 813, 1044, 1087
HpyF10VI GCNNNNNNNGC 6 cut(s) 81, 1010, 1081, 1084, 1117, 1436
HpyF3I CTNAG 9 cut(s) 269, 338, 353, 453, 516, 722, 767, 987, 1208
HpySE526I ACGT 3 cut(s) 465, 735, 966
Hsp92I GRCGYC 2 cut(s) 50, 795
Hsp92II CATG 3 cut(s) 88, 1124, 1234
HspAI GCGC 6 cut(s) 241, 243, 426, 992, 1013, 1068
Kpn2I TCCGGA 1 cut(s) 289
Ksp22I TGATCA 1 cut(s) 492
KspI CCGCGG 2 cut(s) 585, 821
Kzo9I GATC 6 cut(s) 379, 417, 492, 714, 930, 960
LguI GCTCTTC 1 cut(s) 1022
LmnI GCTCC 4 cut(s) 166, 569, 592, 1590
Lsp1109I GCAGC 4 cut(s) 49, 716, 899, 1071
LweI GCATC 1 cut(s) 608
MaeII ACGT 3 cut(s) 465, 735, 966
MaeIII GTNAC 1 cut(s) 1226
MalI GATC 6 cut(s) 381, 419, 494, 716, 932, 962
MbiI CCGCTC 1 cut(s) 1111
MboI GATC 6 cut(s) 379, 417, 492, 714, 930, 960
MhlI GDGCHC 3 cut(s) 566, 805, 972
MlsI TGGCCA 1 cut(s) 1382
MluCI AATT 4 cut(s) 132, 508, 1261, 1568
MluNI TGGCCA 1 cut(s) 1382
MmeI TCCRAC 4 cut(s) 351, 591, 1278, 1494
Mox20I TGGCCA 1 cut(s) 1382
MroI TCCGGA 1 cut(s) 289
MscI TGGCCA 1 cut(s) 1382
MseI TTAA 4 cut(s) 662, 927, 1182, 1334
MslI CAYNNNNRTG 1 cut(s) 616
Msp20I TGGCCA 1 cut(s) 1382
MspA1I CMGCKG 4 cut(s) 357, 487, 584, 820
MspR9I CCNGG 8 cut(s) 94, 209, 442, 758, 799, 800, 856, 1611
MvaI CCWGG 2 cut(s) 758, 856
MvnI CGCG 7 cut(s) 47, 243, 426, 584, 781, 820, 1070
MwoI GCNNNNNNNGC 6 cut(s) 81, 1010, 1081, 1084, 1117, 1436
NciI CCSGG 6 cut(s) 94, 209, 442, 799, 800, 1611
NdeII GATC 6 cut(s) 379, 417, 492, 714, 930, 960
NlaIII CATG 3 cut(s) 88, 1124, 1234
NlaIV GGNNCC 8 cut(s) 207, 362, 439, 588, 803, 982, 1197, 1592
NmeAIII GCCGAG 4 cut(s) 436, 1096, 1106, 1167
NmuCI GTSAC 1 cut(s) 1226
NruI TCGCGA 1 cut(s) 47
NspV TTCGAA 1 cut(s) 741
PaeR7I CTCGAG 1 cut(s) 559
PauI GCGCGC 1 cut(s) 241
PciSI GCTCTTC 1 cut(s) 1022
PcsI WCGNNNNNNNCGW 2 cut(s) 134, 408
PfeI GAWTC 2 cut(s) 572, 1301
Pfl23II CGTACG 1 cut(s) 934
PflFI GACNNNGTC 2 cut(s) 209, 881
PfoI TCCNGGA 1 cut(s) 440
PinAI ACCGGT 2 cut(s) 1167, 1235
PmaCI CACGTG 1 cut(s) 736
PmlI CACGTG 1 cut(s) 736
Ppu21I YACGTR 1 cut(s) 736
Psp124BI GAGCTC 1 cut(s) 566
Psp6I CCWGG 2 cut(s) 756, 854
PspCI CACGTG 1 cut(s) 736
PspGI CCWGG 2 cut(s) 756, 854
PspLI CGTACG 1 cut(s) 934
PspN4I GGNNCC 8 cut(s) 207, 362, 439, 588, 803, 982, 1197, 1592
PspOMI GGGCCC 1 cut(s) 801
PspPI GGNCC 9 cut(s) 13, 205, 342, 754, 801, 802, 1098, 1170, 1287
PspXI VCTCGAGB 1 cut(s) 559
PstNI CAGNNNCTG 1 cut(s) 729
PsyI GACNNNGTC 2 cut(s) 209, 881
PteI GCGCGC 1 cut(s) 241
PvuII CAGCTG 2 cut(s) 357, 487
RruI TCGCGA 1 cut(s) 47
RsaI GTAC 3 cut(s) 860, 936, 1142
RsaNI GTAC 3 cut(s) 859, 935, 1141
RseI CAYNNNNRTG 1 cut(s) 616
Rsr2I CGGWCCG 1 cut(s) 1098
RsrII CGGWCCG 1 cut(s) 1098
SacI GAGCTC 1 cut(s) 566
SacII CCGCGG 2 cut(s) 585, 821
SapI GCTCTTC 1 cut(s) 1022
SaqAI TTAA 4 cut(s) 662, 927, 1182, 1334
Sau3AI GATC 6 cut(s) 379, 417, 492, 714, 930, 960
Sau96I GGNCC 9 cut(s) 13, 205, 342, 754, 801, 802, 1098, 1170, 1287
ScrFI CCNGG 8 cut(s) 94, 209, 442, 758, 799, 800, 856, 1611
SduI GDGCHC 3 cut(s) 566, 805, 972
SfaNI GCATC 1 cut(s) 608
SfcI CTRYAG 1 cut(s) 501
Sfr274I CTCGAG 1 cut(s) 559
Sfr303I CCGCGG 2 cut(s) 585, 821
SfuI TTCGAA 1 cut(s) 741
SgrBI CCGCGG 2 cut(s) 585, 821
SinI GGWCC 5 cut(s) 13, 205, 342, 1098, 1170
SlaI CTCGAG 1 cut(s) 559
SmaI CCCGGG 1 cut(s) 800
SmiMI CAYNNNNRTG 1 cut(s) 616
SmlI CTYRAG 2 cut(s) 559, 971
SmoI CTYRAG 2 cut(s) 559, 971
SrfI GCCCGGGC 1 cut(s) 800
Sse9I AATT 4 cut(s) 132, 508, 1261, 1568
SstI GAGCTC 1 cut(s) 566
StyD4I CCNGG 8 cut(s) 92, 207, 440, 756, 797, 798, 854, 1609
TaaI ACNGT 2 cut(s) 505, 939
TaiI ACGT 3 cut(s) 468, 738, 969
TaqII GACCGA 1 cut(s) 565
TasI AATT 4 cut(s) 132, 508, 1261, 1568
TauI GCSGC 6 cut(s) 157, 304, 524, 587, 1078, 1292
TfiI GAWTC 2 cut(s) 572, 1301
Tru1I TTAA 4 cut(s) 662, 927, 1182, 1334
Tru9I TTAA 4 cut(s) 662, 927, 1182, 1334
TscAI CASTG 3 cut(s) 82, 510, 1015
TseFI GTSAC 1 cut(s) 1226
TseI GCWGC 4 cut(s) 62, 729, 887, 1084
Tsp45I GTSAC 1 cut(s) 1226
TspDTI ATGAA 3 cut(s) 17, 630, 1566
TspGWI ACGGA 5 cut(s) 126, 307, 364, 685, 871
TspMI CCCGGG 1 cut(s) 798
TspRI CASTG 3 cut(s) 82, 510, 1015
Tth111I GACNNNGTC 2 cut(s) 209, 881
VpaK11BI GGWCC 5 cut(s) 13, 205, 342, 1098, 1170
XagI CCTNNNNNAGG 1 cut(s) 766
XapI RAATTY 1 cut(s) 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 1060
XhoI CTCGAG 1 cut(s) 559
XmaI CCCGGG 1 cut(s) 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.