MD09G1040200.v1.1

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008673
Physical Location & Seq
Forward (+)
2527928 .. 2528920
993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1040200.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGGGTTTCTCACCTTCGCCGCCGCAGGCGGCGGCCTGATTTTGATGGGCGCATACGAGGCCATTTCTTCTTCAATCCAAAACCCAAACGCAGATTCAGATTCAGATTCATCTCCGCCGTCGCCGCCTCAAGCTCCGATTCGTCCCCCGCCGCCGCCGAAAACCCAGTCTCGATCGTCGTCGATTTATTACCTCGCCGCCGCCGTTGTTTCGTTGCTGTTCATCTTGAACTCTTTGGTTTCTTTCTTTGATGCGCTCGACTCAGCCGACGGAGTCGGGTCAGCTATGCAATTGCAAGTCCTGGCGGTCGCATCGCTGTTCTTGCTCTACGCGATCACGGGTCTTCTGGTAAAATTCACGAATTCAACTTTGCCTTGTTCGTTGCTTAGTTTGATCGGTCTTTTCGCATTCGTTGAAGAATTTTTAATGTTTTACCTGCAAAGGAAAGACACTTTCGGAATCGAAAATCGGTACTTTGATCTGTTGCTTGTGCCTATTGCTGTGTGTATATTTTCTACAATGCTTGAATTCAACAACCCCAAATCGAATTATCCGAAATTGGCTCGTGGGGTTGGGCTGGTTCTGCAAGGAATGTGGTTTCTGCAAATGGGTATTTCTTTGTACACCGGCTTCATAGCGCACGGATGCTCGTTGCACGAAAAGAGTAGAGGGAATTACACAGTGAAATGTAAGGGGCACCCTGAGTATCACAGGGCTAGAGCCATCGTCACGCTTCAGTTTAACTGCCATCTTGCTTTTATGGTGGTTATGGTTGTGGGAGTGTACTCGATTATCAGTAGGAAGAGTGGCGGAGTTCGGGTTGATGTTTCGCACTATAAGCCGCTTGGCACAGAAATACATCAGTTTGATAATCAGAGCCGGTTTACTTTGGATTCTGATGAGGATGACGAGATTAAAGAGGAGGGGAATTTGGCAATGCAGAGAGCCGGCGAAGTGGAATTGGGTGTGAACGGTTATGGTTCTCACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

331

Amino Acids

36.08

Weight (kDa)

6.06

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 127 - 262 3.7e-45 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 444
AccB1I GGYRCC 1 cut(s) 696
AccII CGCG 1 cut(s) 332
AcsI RAATTY 5 cut(s) 353, 361, 419, 527, 928
AcuI CTGAAG 1 cut(s) 719
AfaI GTAC 3 cut(s) 473, 623, 785
AgsI TTSAA 6 cut(s) 75, 229, 366, 416, 527, 532
AjnI CCWGG 1 cut(s) 300
AjuI GAANNNNNNNTTGG 2 cut(s) 79, 111
AluBI AGCT 2 cut(s) 134, 284
AluI AGCT 2 cut(s) 134, 284
Alw26I GTCTC 1 cut(s) 174
AoxI GGCC 2 cut(s) 34, 60
ApoI RAATTY 5 cut(s) 353, 361, 419, 527, 928
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AspLEI GCGC 3 cut(s) 53, 256, 640
AsuHPI GGTGA 1 cut(s) 4
BaeGI GKGCMC 1 cut(s) 699
BanI GGYRCC 1 cut(s) 696
BarI GAAGNNNNNNTAC 2 cut(s) 614, 646
BauI CACGAG 1 cut(s) 564
BbsI GAAGAC 1 cut(s) 335
BccI CCATC 3 cut(s) 40, 731, 756
BceAI ACGGC 2 cut(s) 103, 188
BciT130I CCWGG 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 1 cut(s) 717
BfuAI ACCTGC 1 cut(s) 444
Bme1390I CCNGG 1 cut(s) 302
BmiI GGNNCC 1 cut(s) 698
BmrFI CCNGG 1 cut(s) 302
BmrI ACTGGG 1 cut(s) 160
BmsI GCATC 3 cut(s) 241, 320, 635
BmuI ACTGGG 1 cut(s) 160
BpiI GAAGAC 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 114
BsaXI ACNNNNNCTCC 2 cut(s) 804, 834
Bse118I RCCGGY 3 cut(s) 626, 879, 947
Bse1I ACTGG 1 cut(s) 166
Bse3DI GCAATG 1 cut(s) 942
BseBI CCWGG 1 cut(s) 302
BseGI GGATG 2 cut(s) 650, 910
BseMI GCAATG 1 cut(s) 942
BseMII CTCAG 2 cut(s) 276, 693
BseNI ACTGG 1 cut(s) 166
BseRI GAGGAG 1 cut(s) 935
BseSI GKGCMC 1 cut(s) 699
Bsh1236I CGCG 1 cut(s) 332
Bsh1285I CGRYCG 2 cut(s) 176, 309
BshFI GGCC 2 cut(s) 36, 62
BshNI GGYRCC 1 cut(s) 696
BsiEI CGRYCG 2 cut(s) 176, 309
BsiSI CCGG 3 cut(s) 627, 880, 948
BslFI GGGAC 1 cut(s) 129
BsmAI GTCTC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 129
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 2 cut(s) 36, 62
Bsp1286I GDGCHC 1 cut(s) 699
Bsp1407I TGTACA 1 cut(s) 621
Bsp143I GATC 4 cut(s) 173, 333, 393, 478
BspANI GGCC 2 cut(s) 36, 62
BspCNI CTCAG 2 cut(s) 275, 694
BspFNI CGCG 1 cut(s) 332
BspLI GGNNCC 1 cut(s) 698
BspMI ACCTGC 1 cut(s) 444
BspT107I GGYRCC 1 cut(s) 696
BsrDI GCAATG 1 cut(s) 942
BsrFI RCCGGY 3 cut(s) 626, 879, 947
BsrGI TGTACA 1 cut(s) 621
BsrI ACTGG 1 cut(s) 166
BssAI RCCGGY 3 cut(s) 626, 879, 947
BssMI GATC 4 cut(s) 173, 333, 393, 478
BssSI CACGAG 1 cut(s) 564
Bst2BI CACGAG 1 cut(s) 564
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 2 cut(s) 682, 974
Bst6I CTCTTC 1 cut(s) 797
BstAUI TGTACA 1 cut(s) 621
BstC8I GCNNGC 2 cut(s) 28, 949
BstDEI CTNAG 3 cut(s) 262, 386, 702
BstF5I GGATG 2 cut(s) 650, 910
BstFNI CGCG 1 cut(s) 332
BstHHI GCGC 3 cut(s) 53, 256, 640
BstKTI GATC 4 cut(s) 176, 336, 396, 481
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 4 cut(s) 173, 333, 393, 478
BstMCI CGRYCG 2 cut(s) 176, 309
BstMWI GCNNNNNNNGC 5 cut(s) 59, 124, 322, 583, 838
BstNI CCWGG 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 699
BstUI CGCG 1 cut(s) 332
BstV2I GAAGAC 1 cut(s) 335
BsuRI GGCC 2 cut(s) 36, 62
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 2 cut(s) 650, 910
BtsIMutI CAGTG 1 cut(s) 687
BveI ACCTGC 1 cut(s) 444
Cac8I GCNNGC 2 cut(s) 28, 949
CfoI GCGC 3 cut(s) 53, 256, 640
Cfr10I RCCGGY 3 cut(s) 626, 879, 947
Csp6I GTAC 3 cut(s) 472, 622, 784
CspCI CAANNNNNGTGG 2 cut(s) 575, 610
CviQI GTAC 3 cut(s) 472, 622, 784
DdeI CTNAG 3 cut(s) 262, 386, 702
DpnI GATC 4 cut(s) 175, 335, 395, 480
DpnII GATC 4 cut(s) 173, 333, 393, 478
Eam1104I CTCTTC 1 cut(s) 797
EarI CTCTTC 1 cut(s) 797
EciI GGCGGA 2 cut(s) 105, 825
Eco57I CTGAAG 1 cut(s) 719
EcoRI GAATTC 2 cut(s) 361, 527
EcoRII CCWGG 1 cut(s) 300
FaiI YATR 8 cut(s) 55, 287, 509, 635, 761, 770, 837, 978
FaqI GGGAC 1 cut(s) 129
FauI CCCGC 1 cut(s) 156
FokI GGATG 2 cut(s) 657, 917
FspBI CTAG 1 cut(s) 717
GlaI GCGC 3 cut(s) 52, 255, 639
HaeIII GGCC 2 cut(s) 36, 62
HapII CCGG 3 cut(s) 627, 880, 948
HhaI GCGC 3 cut(s) 53, 256, 640
Hin6I GCGC 3 cut(s) 51, 254, 638
HinP1I GCGC 3 cut(s) 51, 254, 638
HinfI GANTC 8 cut(s) 95, 101, 107, 139, 260, 273, 459, 893
HpaII CCGG 3 cut(s) 627, 880, 948
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 4 cut(s) 624, 784, 885, 970
Hpy188I TCNGA 7 cut(s) 100, 106, 138, 458, 555, 876, 898
Hpy188III TCNNGA 3 cut(s) 171, 226, 358
Hpy8I GTNNAC 4 cut(s) 624, 784, 885, 970
Hpy99I CGWCG 4 cut(s) 124, 181, 184, 272
HpyAV CCTTC 1 cut(s) 25
HpyCH4III ACNGT 2 cut(s) 682, 974
HpyCH4V TGCA 7 cut(s) 289, 295, 439, 586, 604, 655, 940
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 124, 322, 583, 838
HpyF3I CTNAG 3 cut(s) 262, 386, 702
HspAI GCGC 3 cut(s) 51, 254, 638
KroI GCCGGC 1 cut(s) 947
KroNI GCCGGC 1 cut(s) 949
Kzo9I GATC 4 cut(s) 173, 333, 393, 478
LmnI GCTCC 1 cut(s) 139
LweI GCATC 3 cut(s) 241, 320, 635
MaeI CTAG 1 cut(s) 717
MaeIII GTNAC 1 cut(s) 727
MalI GATC 4 cut(s) 175, 335, 395, 480
MboI GATC 4 cut(s) 173, 333, 393, 478
MboII GAAGA 5 cut(s) 60, 63, 335, 428, 814
MfeI CAATTG 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 699
MlyI GAGTC 2 cut(s) 254, 282
MnlI CCTC 7 cut(s) 52, 138, 203, 662, 895, 913, 916
MroNI GCCGGC 1 cut(s) 947
MseI TTAA 3 cut(s) 425, 741, 915
MspI CCGG 3 cut(s) 627, 880, 948
MspR9I CCNGG 1 cut(s) 302
MunI CAATTG 1 cut(s) 290
Mva1269I GAATGC 1 cut(s) 407
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 1 cut(s) 332
MwoI GCNNNNNNNGC 5 cut(s) 59, 124, 322, 583, 838
NaeI GCCGGC 1 cut(s) 949
NdeII GATC 4 cut(s) 173, 333, 393, 478
NgoMIV GCCGGC 1 cut(s) 947
NlaIV GGNNCC 1 cut(s) 698
NmuCI GTSAC 1 cut(s) 727
PcsI WCGNNNNNNNCGW 3 cut(s) 201, 264, 551
PctI GAATGC 1 cut(s) 407
PdiI GCCGGC 1 cut(s) 949
PfeI GAWTC 6 cut(s) 95, 101, 107, 139, 459, 893
PflFI GACNNNGTC 1 cut(s) 272
Ple19I CGATCG 1 cut(s) 176
PleI GAGTC 2 cut(s) 254, 281
PpsI GAGTC 2 cut(s) 254, 281
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 1 cut(s) 698
PsyI GACNNNGTC 1 cut(s) 272
PvuI CGATCG 1 cut(s) 176
RsaI GTAC 3 cut(s) 473, 623, 785
RsaNI GTAC 3 cut(s) 472, 622, 784
SaqAI TTAA 3 cut(s) 425, 741, 915
Sau3AI GATC 4 cut(s) 173, 333, 393, 478
SchI GAGTC 2 cut(s) 254, 282
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 1 cut(s) 699
SetI ASST 5 cut(s) 17, 136, 195, 286, 438
SfaNI GCATC 3 cut(s) 241, 320, 635
SmlI CTYRAG 1 cut(s) 129
SmoI CTYRAG 1 cut(s) 129
SspMI CTAG 1 cut(s) 717
StyD4I CCNGG 1 cut(s) 300
TaaI ACNGT 2 cut(s) 682, 974
TaqI TCGA 6 cut(s) 172, 182, 258, 462, 545, 788
TaqII GACCGA 1 cut(s) 386
TatI WGTACW 2 cut(s) 621, 783
TfiI GAWTC 6 cut(s) 95, 101, 107, 139, 459, 893
Tru1I TTAA 3 cut(s) 425, 741, 915
Tru9I TTAA 3 cut(s) 425, 741, 915
TscAI CASTG 1 cut(s) 687
TseFI GTSAC 1 cut(s) 727
Tsp45I GTSAC 1 cut(s) 727
TspDTI ATGAA 3 cut(s) 99, 211, 622
TspGWI ACGGA 2 cut(s) 285, 657
TspRI CASTG 1 cut(s) 687
Tth111I GACNNNGTC 1 cut(s) 272
XapI RAATTY 5 cut(s) 353, 361, 419, 527, 928
XspI CTAG 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.