Rorug02G0547600

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008673
Physical Location & Seq
Forward (+)
67553276 .. 67553695
420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0547600.1

Sequence Viewer

Length: 420 bp
ATGGACGACATATTCGGAGAGTCTCTGAACTTGGAAGAGATCCACATCAAGCAAGGCTTCGACGAAGGCCACAAAGACGGCCTGATCGCCGGAAAAGAGGAGGCCAGGCAAGTGGGCCTCAAGGTGGGCTTCGAGGTCGGCGAGGAGCTCGGGTTCTACAAGGGCTGCGTGGACGTATGGAACTCTGCGATCCTGGTCGACCCGACCCGGTTCTCGACCCGGGTCCAGAAGGGCGTGAAGCAGATGGAGGAGTTGGTGGAGAGGTACCCGGTAATGGAACCCGAGAATAAGAGCGTGCAGGAGATAATGGAGGATTTGAGGTTGAAGTTTAAGGCGGTTTCTGCTTCTTTGGGTGTGAAATTGGAGTACAAAGGGTACCCGAGAGCTTCCTCTTCTGGGGCTAAAGACGTTGCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

139

Amino Acids

15.54

Weight (kDa)

5.04

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yae1_N PF09811 19 - 57 1.6e-08 Essential protein Yae1, N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 264, 375
AccB1I GGYRCC 2 cut(s) 264, 375
AccI GTMKAC 1 cut(s) 198
AciI CCGC 1 cut(s) 335
AclWI GGATC 2 cut(s) 34, 184
AfaI GTAC 3 cut(s) 266, 368, 377
AfiI CCNNNNNNNGG 3 cut(s) 124, 274, 396
AgsI TTSAA 1 cut(s) 325
AjnI CCWGG 2 cut(s) 104, 192
AloI GAACNNNNNNTCC 2 cut(s) 137, 169
AluBI AGCT 2 cut(s) 148, 386
AluI AGCT 2 cut(s) 148, 386
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 2 cut(s) 34, 184
Ama87I CYCGRG 4 cut(s) 149, 219, 281, 379
AoxI GGCC 4 cut(s) 67, 79, 102, 115
ApeKI GCWGC 1 cut(s) 165
ArsI GACNNNNNNTTYG 1 cut(s) 28
Asp718I GGTACC 2 cut(s) 264, 375
AspS9I GGNCC 2 cut(s) 115, 223
AsuC2I CCSGG 4 cut(s) 208, 220, 221, 269
AvaI CYCGRG 4 cut(s) 149, 219, 281, 379
AvaII GGWCC 1 cut(s) 223
BanI GGYRCC 2 cut(s) 264, 375
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 1 cut(s) 152
BccI CCATC 1 cut(s) 238
BceAI ACGGC 1 cut(s) 94
BciT130I CCWGG 2 cut(s) 106, 194
BcnI CCSGG 4 cut(s) 208, 220, 221, 269
BcoDI GTCTC 1 cut(s) 27
BisI GCNGC 1 cut(s) 166
BlsI GCNGC 1 cut(s) 167
Bme1390I CCNGG 6 cut(s) 106, 194, 208, 220, 221, 269
Bme18I GGWCC 1 cut(s) 223
BmeT110I CYCGRG 4 cut(s) 149, 219, 281, 379
BmgT120I GGNCC 2 cut(s) 115, 223
BmiI GGNNCC 4 cut(s) 224, 266, 279, 377
BmrFI CCNGG 6 cut(s) 106, 194, 208, 220, 221, 269
BoxI GACNNNNGTC 1 cut(s) 221
BpuEI CTTGAG 1 cut(s) 104
BpuMI CCSGG 4 cut(s) 208, 220, 221, 269
BsaBI GATNNNNATC 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 219
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 274, 396
Bse8I GATNNNNATC 1 cut(s) 44
BseBI CCWGG 2 cut(s) 106, 194
BseDI CCNNGG 1 cut(s) 219
BseJI GATNNNNATC 1 cut(s) 44
BseLI CCNNNNNNNGG 3 cut(s) 124, 274, 396
BseRI GAGGAG 3 cut(s) 113, 158, 263
BseXI GCAGC 1 cut(s) 152
BsgI GTGCAG 1 cut(s) 317
BshFI GGCC 4 cut(s) 69, 81, 104, 117
BshNI GGYRCC 2 cut(s) 264, 375
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 4 cut(s) 149, 219, 281, 379
BsiSI CCGG 4 cut(s) 90, 208, 220, 269
BslI CCNNNNNNNGG 3 cut(s) 124, 274, 396
BsmAI GTCTC 1 cut(s) 27
BsnI GGCC 4 cut(s) 69, 81, 104, 117
BsoBI CYCGRG 4 cut(s) 149, 219, 281, 379
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 3 cut(s) 39, 84, 189
BspACI CCGC 1 cut(s) 335
BspANI GGCC 4 cut(s) 69, 81, 104, 117
BspLI GGNNCC 4 cut(s) 224, 266, 279, 377
BspPI GGATC 2 cut(s) 34, 184
BspT107I GGYRCC 2 cut(s) 264, 375
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 3 cut(s) 39, 84, 189
Bst2UI CCWGG 2 cut(s) 106, 194
Bst6I CTCTTC 2 cut(s) 30, 397
BstC8I GCNNGC 1 cut(s) 296
BstKTI GATC 3 cut(s) 42, 87, 192
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 3 cut(s) 39, 84, 189
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNI CCWGG 2 cut(s) 106, 194
BstPAI GACNNNNGTC 1 cut(s) 221
BstSCI CCNGG 6 cut(s) 104, 192, 206, 218, 219, 267
BstV1I GCAGC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 39
BstXI CCANNNNNNTGG 1 cut(s) 112
BstYI RGATCY 1 cut(s) 39
BsuRI GGCC 4 cut(s) 69, 81, 104, 117
Cac8I GCNNGC 1 cut(s) 296
Cfr13I GGNCC 2 cut(s) 115, 223
Cfr9I CCCGGG 1 cut(s) 219
Csp6I GTAC 3 cut(s) 265, 367, 376
CviQI GTAC 3 cut(s) 265, 367, 376
DpnI GATC 3 cut(s) 41, 86, 191
DpnII GATC 3 cut(s) 39, 84, 189
Eam1104I CTCTTC 2 cut(s) 30, 397
EarI CTCTTC 2 cut(s) 30, 397
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 148
Eco88I CYCGRG 4 cut(s) 149, 219, 281, 379
EcoICRI GAGCTC 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 104, 192
EcoT38I GRGCYC 1 cut(s) 150
FaiI YATR 2 cut(s) 11, 178
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 113, 145
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 1 cut(s) 166
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 166
GluI GCNGC 1 cut(s) 166
HaeIII GGCC 4 cut(s) 69, 81, 104, 117
HapII CCGG 4 cut(s) 90, 208, 220, 269
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 1 cut(s) 20
HpaII CCGG 4 cut(s) 90, 208, 220, 269
Hpy166II GTNNAC 2 cut(s) 172, 199
Hpy188I TCNGA 2 cut(s) 17, 27
Hpy188III TCNNGA 2 cut(s) 214, 226
Hpy8I GTNNAC 2 cut(s) 172, 199
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 2 cut(s) 59, 223
HpyCH4IV ACGT 2 cut(s) 174, 408
HpyCH4V TGCA 1 cut(s) 298
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpySE526I ACGT 2 cut(s) 174, 408
KpnI GGTACC 2 cut(s) 268, 379
Kzo9I GATC 3 cut(s) 39, 84, 189
LmnI GCTCC 1 cut(s) 145
Lsp1109I GCAGC 1 cut(s) 152
MaeII ACGT 2 cut(s) 174, 408
MalI GATC 3 cut(s) 41, 86, 191
MboI GATC 3 cut(s) 39, 84, 189
MboII GAAGA 2 cut(s) 47, 384
MflI RGATCY 1 cut(s) 39
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 1 cut(s) 359
MlyI GAGTC 1 cut(s) 29
MseI TTAA 1 cut(s) 330
MspI CCGG 4 cut(s) 90, 208, 220, 269
MspR9I CCNGG 6 cut(s) 106, 194, 208, 220, 221, 269
MvaI CCWGG 2 cut(s) 106, 194
MwoI GCNNNNNNNGC 1 cut(s) 341
NciI CCSGG 4 cut(s) 208, 220, 221, 269
NdeII GATC 3 cut(s) 39, 84, 189
NlaIV GGNNCC 4 cut(s) 224, 266, 279, 377
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PkrI GCNGC 1 cut(s) 167
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PshAI GACNNNNGTC 1 cut(s) 221
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 2 cut(s) 104, 192
PspGI CCWGG 2 cut(s) 104, 192
PspN4I GGNNCC 4 cut(s) 224, 266, 279, 377
PspPI GGNCC 2 cut(s) 115, 223
PsuI RGATCY 1 cut(s) 39
RsaI GTAC 3 cut(s) 266, 368, 377
RsaNI GTAC 3 cut(s) 265, 367, 376
SacI GAGCTC 1 cut(s) 150
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 1 cut(s) 166
Sau3AI GATC 3 cut(s) 39, 84, 189
Sau96I GGNCC 2 cut(s) 115, 223
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 6 cut(s) 106, 194, 208, 220, 221, 269
SduI GDGCHC 1 cut(s) 150
SetI ASST 8 cut(s) 126, 138, 150, 177, 266, 323, 388, 411
SinI GGWCC 1 cut(s) 223
SmaI CCCGGG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
Sse9I AATT 1 cut(s) 359
SsiI CCGC 1 cut(s) 335
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 6 cut(s) 104, 192, 206, 218, 219, 267
TaiI ACGT 2 cut(s) 177, 411
TaqI TCGA 4 cut(s) 60, 132, 198, 215
TasI AATT 1 cut(s) 359
TatI WGTACW 1 cut(s) 366
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TseI GCWGC 1 cut(s) 165
TspMI CCCGGG 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 223
XmaI CCCGGG 1 cut(s) 219
XmiI GTMKAC 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.